FvH4_6g45551

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000735
Physical Location & Seq
Reverse (-)
34950104 .. 34950562
459 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45551.t1

Sequence Viewer

Length: 459 bp
ATGGCTATGGCCAGTGACTACTGGAAAGTGGTGGGCGATGCTTTCTGCAACGTACTTGTCGGCTTCACAGAACCAGAAAACGAGTTTCTATGCAGCAACACCAATACGTTCTTGATGGAATTTGAGGCGGTGATCAGACGTGTCACCTCCGACGAGGCGGACCGACTCCTAGACGACGACGTCATCCCCCGCGAGATAGTGAATACGACCTCAATCGAACTTGACGACTCTGACTCATCGTACATGGAGGCAACCCTATCCGAAACGCTTCAACAACTACAAGTCCCACTGGAGGAGCGTAACTTCATGGTGGAGAATGTAATGTTGAAAGCCTCCGAGGCGGCACGAAATCTCAGTCCTTGTGCCGGTGGGAAGAGACTGCGTATGAGGGTGAACATCGATATGTATGTGATCGATGATGATGATGATGTCGATGGGGGTGGTATGATTGTCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.08

Weight (kDa)

4.05

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
AatII GACGTC 1 cut(s) 183
AccII CGCG 1 cut(s) 192
AciI CCGC 4 cut(s) 128, 158, 190, 341
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 119
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 2 cut(s) 54, 242
AfiI CCNNNNNNNGG 2 cut(s) 292, 365
AflIII ACRYGT 1 cut(s) 139
AgsI TTSAA 2 cut(s) 272, 328
AjiI CACGTC 1 cut(s) 140
Alw26I GTCTC 1 cut(s) 370
AoxI GGCC 1 cut(s) 9
ApeKI GCWGC 1 cut(s) 93
ApoI RAATTY 1 cut(s) 119
ArsI GACNNNNNNTTYG 2 cut(s) 340, 372
Asp700I GAANNNNTTC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 160
AsuHPI GGTGA 3 cut(s) 136, 142, 403
AvaII GGWCC 1 cut(s) 160
BalI TGGCCA 1 cut(s) 11
BbvI GCAGC 1 cut(s) 105
BccI CCATC 2 cut(s) 109, 428
BclI TGATCA 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 370
BfaI CTAG 1 cut(s) 170
BglI GCCNNNNNGGC 1 cut(s) 338
BisI GCNGC 2 cut(s) 94, 342
BlsI GCNGC 2 cut(s) 95, 343
Bme18I GGWCC 1 cut(s) 160
BmgBI CACGTC 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 160
BmsI GCATC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 311
Bsa29I ATCGAT 2 cut(s) 399, 414
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 336
Bsc4I CCNNNNNNNGG 2 cut(s) 292, 365
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 3 cut(s) 12, 26, 294
BseCI ATCGAT 2 cut(s) 399, 414
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 1 cut(s) 183
BseLI CCNNNNNNNGG 2 cut(s) 292, 365
BseMII CTCAG 1 cut(s) 367
BseNI ACTGG 3 cut(s) 12, 26, 294
BseRI GAGGAG 1 cut(s) 308
BseXI GCAGC 1 cut(s) 105
Bsh1236I CGCG 1 cut(s) 192
BshFI GGCC 1 cut(s) 11
BshVI ATCGAT 2 cut(s) 399, 414
BsiSI CCGG 1 cut(s) 366
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 2 cut(s) 292, 365
BsmAI GTCTC 1 cut(s) 370
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 1 cut(s) 11
Bsp143I GATC 2 cut(s) 132, 411
BspACI CCGC 4 cut(s) 128, 158, 190, 341
BspANI GGCC 1 cut(s) 11
BspCNI CTCAG 1 cut(s) 366
BspDI ATCGAT 2 cut(s) 399, 414
BspFNI CGCG 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 3 cut(s) 12, 26, 294
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 2 cut(s) 132, 411
BssNI GRCGYC 1 cut(s) 180
Bst6I CTCTTC 1 cut(s) 368
BstACI GRCGYC 1 cut(s) 180
BstDEI CTNAG 1 cut(s) 353
BstF5I GGATG 1 cut(s) 183
BstFNI CGCG 1 cut(s) 192
BstKTI GATC 2 cut(s) 135, 414
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 2 cut(s) 132, 411
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 105
Bsu15I ATCGAT 2 cut(s) 399, 414
BsuRI GGCC 1 cut(s) 11
BsuTUI ATCGAT 2 cut(s) 399, 414
BtgZI GCGATG 1 cut(s) 51
BtrI CACGTC 1 cut(s) 140
BtsCI GGATG 1 cut(s) 183
BtsIMutI CAGTG 2 cut(s) 19, 287
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 160
ClaI ATCGAT 2 cut(s) 399, 414
CpoI CGGWCCG 1 cut(s) 160
Csp6I GTAC 2 cut(s) 53, 241
CspI CGGWCCG 1 cut(s) 160
CviAII CATG 2 cut(s) 244, 307
CviJI RGCY 4 cut(s) 5, 11, 63, 332
CviKI_1 RGCY 4 cut(s) 5, 11, 63, 332
CviQI GTAC 2 cut(s) 53, 241
DdeI CTNAG 1 cut(s) 353
DpnI GATC 2 cut(s) 134, 413
DpnII GATC 2 cut(s) 132, 411
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
EciI GGCGGA 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 160
FaeI CATG 2 cut(s) 247, 310
FaiI YATR 8 cut(s) 8, 91, 245, 308, 386, 404, 408, 446
FaqI GGGAC 1 cut(s) 269
FatI CATG 2 cut(s) 243, 306
FauI CCCGC 1 cut(s) 197
FbaI TGATCA 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 94, 342
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 2 cut(s) 94, 342
FspBI CTAG 1 cut(s) 170
GluI GCNGC 2 cut(s) 94, 342
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 1 cut(s) 11
HapII CCGG 1 cut(s) 366
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 2 cut(s) 247, 310
HinfI GANTC 3 cut(s) 165, 227, 233
HpaII CCGG 1 cut(s) 366
HphI GGTGA 3 cut(s) 136, 142, 403
Hpy166II GTNNAC 1 cut(s) 394
Hpy188I TCNGA 5 cut(s) 137, 151, 232, 262, 337
Hpy188III TCNNGA 1 cut(s) 112
Hpy8I GTNNAC 1 cut(s) 394
Hpy99I CGWCG 3 cut(s) 155, 179, 182
HpyCH4IV ACGT 4 cut(s) 51, 107, 139, 180
HpyCH4V TGCA 2 cut(s) 48, 93
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 1 cut(s) 353
HpySE526I ACGT 4 cut(s) 51, 107, 139, 180
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 2 cut(s) 247, 310
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 2 cut(s) 132, 411
LmnI GCTCC 1 cut(s) 295
LpnPI CCDG 5 cut(s) 7, 25, 87, 275, 379
Lsp1109I GCAGC 1 cut(s) 105
LweI GCATC 1 cut(s) 28
MaeI CTAG 1 cut(s) 170
MaeII ACGT 4 cut(s) 51, 107, 139, 180
MaeIII GTNAC 3 cut(s) 14, 142, 299
MalI GATC 2 cut(s) 134, 413
MboI GATC 2 cut(s) 132, 411
MboII GAAGA 1 cut(s) 385
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 1 cut(s) 119
MluNI TGGCCA 1 cut(s) 11
MlyI GAGTC 3 cut(s) 159, 221, 227
MmeI TCCRAC 1 cut(s) 174
MnlI CCTC 9 cut(s) 118, 148, 157, 220, 241, 286, 331, 343, 381
Mox20I TGGCCA 1 cut(s) 11
MroXI GAANNNNTTC 1 cut(s) 267
MscI TGGCCA 1 cut(s) 11
MslI CAYNNNNRTG 1 cut(s) 401
Msp20I TGGCCA 1 cut(s) 11
MspI CCGG 1 cut(s) 366
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 1 cut(s) 338
NdeII GATC 2 cut(s) 132, 411
NlaIII CATG 2 cut(s) 247, 310
NmuCI GTSAC 2 cut(s) 14, 142
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 267
PflFI GACNNNGTC 1 cut(s) 179
PkrI GCNGC 2 cut(s) 95, 343
PleI GAGTC 3 cut(s) 159, 221, 227
PpsI GAGTC 3 cut(s) 159, 221, 227
PspPI GGNCC 1 cut(s) 160
PsyI GACNNNGTC 1 cut(s) 179
RsaI GTAC 2 cut(s) 54, 242
RsaNI GTAC 2 cut(s) 53, 241
RseI CAYNNNNRTG 1 cut(s) 401
Rsr2I CGGWCCG 1 cut(s) 160
RsrII CGGWCCG 1 cut(s) 160
SatI GCNGC 2 cut(s) 94, 342
Sau3AI GATC 2 cut(s) 132, 411
Sau96I GGNCC 1 cut(s) 160
SchI GAGTC 3 cut(s) 159, 221, 227
SetI ASST 6 cut(s) 54, 110, 142, 149, 183, 212
SfaNI GCATC 1 cut(s) 28
SinI GGWCC 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 401
Sse9I AATT 1 cut(s) 119
SsiI CCGC 4 cut(s) 128, 158, 190, 341
SspMI CTAG 1 cut(s) 170
TaiI ACGT 4 cut(s) 54, 110, 142, 183
TaqI TCGA 5 cut(s) 216, 399, 414, 432, 453
TaqII GACCGA 1 cut(s) 177
TasI AATT 1 cut(s) 119
TauI GCSGC 1 cut(s) 344
TscAI CASTG 2 cut(s) 19, 294
TseFI GTSAC 2 cut(s) 14, 142
TseI GCWGC 1 cut(s) 93
Tsp45I GTSAC 2 cut(s) 14, 142
TspDTI ATGAA 1 cut(s) 295
TspRI CASTG 2 cut(s) 19, 294
Tth111I GACNNNGTC 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 160
XapI RAATTY 1 cut(s) 119
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 1 cut(s) 170
ZraI GACGTC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.