Rorug05G0315100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000735
Physical Location & Seq
Forward (+)
37411619 .. 37412562
944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0315100.1

Sequence Viewer

Length: 405 bp
ATGAAGAGAACCCACTACTCGTTGATGGAGGAAAAACCCAACAGCAATGTTAACAACACCACCAACCAGATCAAGGTTCCTGCATTCTCTGGGAAAAGAAGCAAACACTGCGGCGGCGGAGGCCACAACGAGCTTGTGGGGTTACATAACATGTCTGCGGCAGACATGAGCTCGGGACGCGAGAGGCTGAAGAAACACCGGGAGGAGGTTGCCGGGCATGTGACGATACCGGATTCGTGGGGGCAGGAGGAGCTGCTCAAGGAGTGGATCGATTACTCTCCGTTTGATGCGTTGCTGGTTCCGACAGGGCTCAGCTCGGCTCGTGAAGCACTTGCTAGGGAAGGACGTCGTCGAGCAAGTAATAATAATGCTCAAGGAGTCATGAGGATCGAGAGTAGGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

134

Amino Acids

14.93

Weight (kDa)

9.3

Isoelectric Point (pI)

60.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 349
AccII CGCG 1 cut(s) 180
AciI CCGC 4 cut(s) 111, 114, 117, 158
AclWI GGATC 2 cut(s) 275, 395
AcuI CTGAAG 1 cut(s) 209
AcyI GRCGYC 1 cut(s) 346
AfiI CCNNNNNNNGG 2 cut(s) 73, 205
AflIII ACRYGT 1 cut(s) 150
AluBI AGCT 4 cut(s) 133, 171, 253, 315
AluI AGCT 4 cut(s) 133, 171, 253, 315
Alw21I GWGCWC 1 cut(s) 173
AlwI GGATC 2 cut(s) 275, 395
Ama87I CYCGRG 1 cut(s) 172
AoxI GGCC 1 cut(s) 121
ApeKI GCWGC 1 cut(s) 253
AsuC2I CCSGG 2 cut(s) 200, 214
AvaI CYCGRG 1 cut(s) 172
BanII GRGCYC 2 cut(s) 173, 312
BauI CACGAG 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 1 cut(s) 240
BccI CCATC 1 cut(s) 19
BcnI CCSGG 2 cut(s) 200, 214
BfaI CTAG 1 cut(s) 336
BisI GCNGC 4 cut(s) 112, 115, 159, 254
BlpI GCTNAGC 1 cut(s) 311
BlsI GCNGC 4 cut(s) 113, 116, 160, 255
Bme1390I CCNGG 2 cut(s) 200, 214
BmeT110I CYCGRG 1 cut(s) 172
BmiI GGNNCC 2 cut(s) 78, 300
BmrFI CCNGG 2 cut(s) 200, 214
BmsI GCATC 1 cut(s) 277
Bpu1102I GCTNAGC 1 cut(s) 311
BpuEI CTTGAG 2 cut(s) 242, 357
BpuMI CCSGG 2 cut(s) 200, 214
Bsa29I ATCGAT 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 346
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 205
Bse3DI GCAATG 1 cut(s) 52
BseCI ATCGAT 1 cut(s) 270
BseLI CCNNNNNNNGG 2 cut(s) 73, 205
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 325
BseRI GAGGAG 2 cut(s) 218, 263
BseXI GCAGC 1 cut(s) 240
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 1 cut(s) 123
BshVI ATCGAT 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 173
BsiHKCI CYCGRG 1 cut(s) 172
BsiSI CCGG 3 cut(s) 199, 213, 230
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 2 cut(s) 73, 205
BsmFI GGGAC 1 cut(s) 189
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 172
Bsp1286I GDGCHC 2 cut(s) 173, 312
Bsp143I GATC 3 cut(s) 69, 267, 387
Bsp1720I GCTNAGC 1 cut(s) 311
BspACI CCGC 4 cut(s) 111, 114, 117, 158
BspANI GGCC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 324
BspDI ATCGAT 1 cut(s) 270
BspFNI CGCG 1 cut(s) 180
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 2 cut(s) 78, 300
BspPI GGATC 2 cut(s) 275, 395
BsrDI GCAATG 1 cut(s) 52
BssMI GATC 3 cut(s) 69, 267, 387
BssNI GRCGYC 1 cut(s) 346
BssSI CACGAG 1 cut(s) 321
Bst2BI CACGAG 1 cut(s) 321
BstACI GRCGYC 1 cut(s) 346
BstAPI GCANNNNNTGC 1 cut(s) 108
BstDEI CTNAG 1 cut(s) 311
BstFNI CGCG 1 cut(s) 180
BstKTI GATC 3 cut(s) 72, 270, 390
BstMBI GATC 3 cut(s) 69, 267, 387
BstMWI GCNNNNNNNGC 5 cut(s) 108, 120, 177, 250, 326
BstNSI RCATGY 2 cut(s) 154, 221
BstSCI CCNGG 2 cut(s) 198, 212
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 1 cut(s) 240
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 1 cut(s) 123
BsuTUI ATCGAT 1 cut(s) 270
BtsI GCAGTG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 106
CciI TCATGA 1 cut(s) 381
ClaI ATCGAT 1 cut(s) 270
CseI GACGC 1 cut(s) 186
CviAII CATG 4 cut(s) 151, 166, 218, 382
CviJI RGCY 8 cut(s) 123, 133, 171, 187, 253, 310, 315, 320
CviKI_1 RGCY 8 cut(s) 123, 133, 171, 187, 253, 310, 315, 320
DdeI CTNAG 1 cut(s) 311
DpnI GATC 3 cut(s) 71, 269, 389
DpnII GATC 3 cut(s) 69, 267, 387
EciI GGCGGA 1 cut(s) 132
Ecl136II GAGCTC 1 cut(s) 171
Eco24I GRGCYC 2 cut(s) 173, 312
Eco53kI GAGCTC 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 172
EcoICRI GAGCTC 1 cut(s) 171
EcoT38I GRGCYC 2 cut(s) 173, 312
FaeI CATG 4 cut(s) 154, 169, 221, 385
FaiI YATR 5 cut(s) 147, 152, 167, 219, 383
FaqI GGGAC 1 cut(s) 189
FatI CATG 4 cut(s) 150, 165, 217, 381
Fnu4HI GCNGC 4 cut(s) 112, 115, 159, 254
FriOI GRGCYC 2 cut(s) 173, 312
Fsp4HI GCNGC 4 cut(s) 112, 115, 159, 254
FspBI CTAG 1 cut(s) 336
GluI GCNGC 4 cut(s) 112, 115, 159, 254
HaeIII GGCC 1 cut(s) 123
HapII CCGG 3 cut(s) 199, 213, 230
HgaI GACGC 1 cut(s) 186
Hin1I GRCGYC 1 cut(s) 346
Hin1II CATG 4 cut(s) 154, 169, 221, 385
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 2 cut(s) 233, 378
HpaI GTTAAC 1 cut(s) 52
HpaII CCGG 3 cut(s) 199, 213, 230
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 4 cut(s) 174, 323, 382, 391
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 2 cut(s) 351, 354
HpyAV CCTTC 1 cut(s) 335
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 1 cut(s) 83
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 120, 177, 250, 326
HpyF3I CTNAG 1 cut(s) 311
HpySE526I ACGT 1 cut(s) 346
Hsp92I GRCGYC 1 cut(s) 346
Hsp92II CATG 4 cut(s) 154, 169, 221, 385
KspAI GTTAAC 1 cut(s) 52
Kzo9I GATC 3 cut(s) 69, 267, 387
LmnI GCTCC 1 cut(s) 250
LpnPI CCDG 9 cut(s) 75, 80, 93, 212, 226, 230, 243, 281, 291
Lsp1109I GCAGC 1 cut(s) 240
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 336
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 2 cut(s) 141, 220
MalI GATC 3 cut(s) 71, 269, 389
MboI GATC 3 cut(s) 69, 267, 387
MboII GAAGA 2 cut(s) 16, 202
MhlI GDGCHC 2 cut(s) 173, 312
MlyI GAGTC 1 cut(s) 387
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 7 cut(s) 22, 113, 177, 196, 199, 241, 378
MseI TTAA 1 cut(s) 51
MspI CCGG 3 cut(s) 199, 213, 230
MspR9I CCNGG 2 cut(s) 200, 214
Mva1269I GAATGC 1 cut(s) 83
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 5 cut(s) 108, 120, 177, 250, 326
NciI CCSGG 2 cut(s) 200, 214
NdeII GATC 3 cut(s) 69, 267, 387
NlaIII CATG 4 cut(s) 154, 169, 221, 385
NlaIV GGNNCC 2 cut(s) 78, 300
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 1 cut(s) 220
NspI RCATGY 2 cut(s) 154, 221
PagI TCATGA 1 cut(s) 381
PciI ACATGT 1 cut(s) 150
PctI GAATGC 1 cut(s) 83
PfeI GAWTC 1 cut(s) 233
PflFI GACNNNGTC 1 cut(s) 348
PkrI GCNGC 4 cut(s) 113, 116, 160, 255
PleI GAGTC 1 cut(s) 386
PpsI GAGTC 1 cut(s) 386
PscI ACATGT 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 173
PspN4I GGNNCC 2 cut(s) 78, 300
PsyI GACNNNGTC 1 cut(s) 348
SacI GAGCTC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 4 cut(s) 112, 115, 159, 254
Sau3AI GATC 3 cut(s) 69, 267, 387
SchI GAGTC 1 cut(s) 387
ScrFI CCNGG 2 cut(s) 200, 214
SduI GDGCHC 2 cut(s) 173, 312
SetI ASST 8 cut(s) 78, 135, 173, 210, 255, 317, 349, 401
SfaNI GCATC 1 cut(s) 277
SmlI CTYRAG 2 cut(s) 257, 372
SmoI CTYRAG 2 cut(s) 257, 372
SsiI CCGC 4 cut(s) 111, 114, 117, 158
SspMI CTAG 1 cut(s) 336
SstI GAGCTC 1 cut(s) 173
StyD4I CCNGG 2 cut(s) 198, 212
TaiI ACGT 1 cut(s) 349
TaqI TCGA 3 cut(s) 270, 352, 390
TauI GCSGC 3 cut(s) 114, 117, 161
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 1 cut(s) 113
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 1 cut(s) 113
Tth111I GACNNNGTC 1 cut(s) 348
XceI RCATGY 2 cut(s) 154, 221
XspI CTAG 1 cut(s) 336
ZraI GACGTC 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.