MD07G1074300.v1.1

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000735
Physical Location & Seq
Forward (+)
7081789 .. 7082511
723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1074300.v1.1.491

Sequence Viewer

Length: 723 bp
ATGGCAAGTCACGGAAGGGAATTAGTGATGGGTGAGCCTTATTGTTATACATGGGTTCATGGCATTGATGAATTCGAAGACAGCATACAAAGCACGCATTTCGTGATGTATATCAAGGCGACAATCAGACGCCAGCAGCCTAGGGTTTCAGAGGAAGTGGAAGAAGAGGACGCAGAAATAGAAGAACACTTTAGTGTGAATGATGCCACAATGGAACTACAATTTGACCCAGACAGTACTGACATGGGTATTCTCATATCCAACACACTTTCGGGCCTTCATATTCCTCTCCAGGCTCATCCACCCATAGTAGCTGAAATATTGTATAAAGCCTACTCCTGGGCAACGGATAATGATAGTTATTTTCCTCCTAGTCGAGAGGTTTTTCATATGGGGGTGTACGTGGAAGTTAGGACATACCTTTCAGATATTGGGGACACTGACATGGACGTGTCAGATATTGGGGACACTGACATGGACGTAGAGGATGGTGGTGATGAGCCTCAATTTGTACCGGCAAGTAAATCGTCCATTGAGGAGTTGGAGAGGACGATAGTCGAAACCTCAACAATGTGTTCTATTTGCAGGGAGGAGATGATGGTTGGTTCAGAAGCAACTCGGATGCCGTGCTCACATCTTTACCATGGAGATTGTATTGTGGAGTGGCTGCAGAGAAGCAGGATTTGCCCGTTGTGTAGGTACTCCATGCCTGCTGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

241

Amino Acids

27.33

Weight (kDa)

4.3

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 191 - 233 5e-14 Ring finger domain
zf-C3HC4_2 PF13923 191 - 232 2.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 192 - 219 2e-08 RING-like zinc finger
zf-rbx1 PF12678 192 - 233 9.8e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 192 - 232 1.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 192 - 230 8.8e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 71
AcyI GRCGYC 1 cut(s) 130
AfaI GTAC 4 cut(s) 238, 401, 513, 701
AfiI CCNNNNNNNGG 1 cut(s) 339
AflIII ACRYGT 1 cut(s) 450
AjiI CACGTC 1 cut(s) 451
AjnI CCWGG 2 cut(s) 291, 338
AleI CACNNNNGTG 1 cut(s) 192
AluBI AGCT 1 cut(s) 314
AluI AGCT 1 cut(s) 314
Alw21I GWGCWC 1 cut(s) 632
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 2 cut(s) 136, 667
ApoI RAATTY 1 cut(s) 71
AspA2I CCTAGG 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 274
AsuHPI GGTGA 2 cut(s) 44, 506
AsuII TTCGAA 1 cut(s) 75
AvrII CCTAGG 1 cut(s) 140
BarI GAAGNNNNNNTAC 2 cut(s) 69, 101
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 632
BbvI GCAGC 2 cut(s) 148, 654
BccI CCATC 3 cut(s) 22, 482, 592
BceAI ACGGC 1 cut(s) 610
BcgI CGANNNNNNTGC 4 cut(s) 82, 116, 507, 541
BciT130I CCWGG 2 cut(s) 293, 340
BfaI CTAG 2 cut(s) 141, 372
BfmI CTRYAG 1 cut(s) 668
BisI GCNGC 2 cut(s) 137, 668
BlnI CCTAGG 1 cut(s) 140
BlsI GCNGC 2 cut(s) 138, 669
BmcAI AGTACT 1 cut(s) 238
Bme1390I CCNGG 2 cut(s) 293, 340
BmgBI CACGTC 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 274
BmrFI CCNGG 2 cut(s) 293, 340
BmsI GCATC 2 cut(s) 193, 612
BoxI GACNNNNGTC 1 cut(s) 554
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 275
Bpu14I TTCGAA 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 403
BsaBI GATNNNNATC 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 130
BsaJI CCNNGG 3 cut(s) 140, 339, 643
Bsc4I CCNNNNNNNGG 1 cut(s) 339
Bse118I RCCGGY 1 cut(s) 514
Bse8I GATNNNNATC 1 cut(s) 110
BseBI CCWGG 2 cut(s) 293, 340
BseDI CCNNGG 3 cut(s) 140, 339, 643
BseGI GGATG 3 cut(s) 298, 493, 627
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 1 cut(s) 339
BseRI GAGGAG 2 cut(s) 551, 605
BseXI GCAGC 2 cut(s) 148, 654
BshFI GGCC 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 632
BsiSI CCGG 1 cut(s) 515
BslFI GGGAC 2 cut(s) 449, 479
BslI CCNNNNNNNGG 1 cut(s) 339
BsmFI GGGAC 2 cut(s) 449, 479
BsnI GGCC 1 cut(s) 276
Bsp119I TTCGAA 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 632
Bsp19I CCATGG 1 cut(s) 643
BspANI GGCC 1 cut(s) 276
BspMAI CTGCAG 1 cut(s) 672
BspT104I TTCGAA 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 514
BssAI RCCGGY 1 cut(s) 514
BssECI CCNNGG 3 cut(s) 140, 339, 643
BssNI GRCGYC 1 cut(s) 130
BssT1I CCWWGG 2 cut(s) 140, 643
Bst2UI CCWGG 2 cut(s) 293, 340
Bst4CI ACNGT 1 cut(s) 236
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 130
BstAPI GCANNNNNTGC 1 cut(s) 684
BstBAI YACGTR 1 cut(s) 403
BstBI TTCGAA 1 cut(s) 75
BstC8I GCNNGC 3 cut(s) 95, 134, 711
BstDSI CCRYGG 1 cut(s) 643
BstF5I GGATG 3 cut(s) 298, 493, 627
BstMWI GCNNNNNNNGC 2 cut(s) 90, 684
BstNI CCWGG 2 cut(s) 293, 340
BstPAI GACNNNNGTC 1 cut(s) 554
BstSCI CCNGG 2 cut(s) 291, 338
BstSFI CTRYAG 1 cut(s) 668
BstV1I GCAGC 2 cut(s) 148, 654
BstV2I GAAGAC 1 cut(s) 84
BsuRI GGCC 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 643
BtrI CACGTC 1 cut(s) 451
BtsCI GGATG 3 cut(s) 298, 493, 627
BtsIMutI CAGTG 2 cut(s) 438, 468
Cac8I GCNNGC 3 cut(s) 95, 134, 711
Cfr10I RCCGGY 1 cut(s) 514
Cfr13I GGNCC 1 cut(s) 274
CseI GACGC 2 cut(s) 138, 179
Csp6I GTAC 4 cut(s) 237, 400, 512, 700
CviAII CATG 7 cut(s) 51, 59, 244, 445, 475, 644, 706
CviJI RGCY 8 cut(s) 37, 139, 276, 296, 314, 332, 502, 667
CviKI_1 RGCY 8 cut(s) 37, 139, 276, 296, 314, 332, 502, 667
CviQI GTAC 4 cut(s) 237, 400, 512, 700
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 140, 643
EcoRI GAATTC 1 cut(s) 71
EcoRII CCWGG 2 cut(s) 291, 338
EcoT14I CCWWGG 2 cut(s) 140, 643
ErhI CCWWGG 2 cut(s) 140, 643
FaeI CATG 7 cut(s) 54, 62, 247, 448, 478, 647, 709
FaqI GGGAC 2 cut(s) 449, 479
FatI CATG 7 cut(s) 50, 58, 243, 444, 474, 643, 705
FauNDI CATATG 1 cut(s) 390
Fnu4HI GCNGC 2 cut(s) 137, 668
FokI GGATG 3 cut(s) 285, 500, 634
Fsp4HI GCNGC 2 cut(s) 137, 668
FspBI CTAG 2 cut(s) 141, 372
GluI GCNGC 2 cut(s) 137, 668
GsuI CTGGAG 1 cut(s) 275
HaeIII GGCC 1 cut(s) 276
HapII CCGG 1 cut(s) 515
HgaI GACGC 2 cut(s) 138, 179
Hin1I GRCGYC 1 cut(s) 130
Hin1II CATG 7 cut(s) 54, 62, 247, 448, 478, 647, 709
HpaII CCGG 1 cut(s) 515
HphI GGTGA 2 cut(s) 44, 506
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 6 cut(s) 128, 151, 427, 457, 610, 621
Hpy188III TCNNGA 2 cut(s) 103, 377
Hpy8I GTNNAC 1 cut(s) 400
HpyAV CCTTC 2 cut(s) 9, 287
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4IV ACGT 3 cut(s) 402, 450, 480
HpyCH4V TGCA 2 cut(s) 585, 670
HpyF10VI GCNNNNNNNGC 2 cut(s) 90, 684
HpySE526I ACGT 3 cut(s) 402, 450, 480
Hsp92I GRCGYC 1 cut(s) 130
Hsp92II CATG 7 cut(s) 54, 62, 247, 448, 478, 647, 709
LpnPI CCDG 9 cut(s) 146, 243, 278, 305, 325, 352, 528, 571, 664
Lsp1109I GCAGC 2 cut(s) 148, 654
LweI GCATC 2 cut(s) 193, 612
MaeI CTAG 2 cut(s) 141, 372
MaeII ACGT 3 cut(s) 402, 450, 480
MaeIII GTNAC 1 cut(s) 8
MboII GAAGA 4 cut(s) 89, 173, 176, 194
MhlI GDGCHC 1 cut(s) 632
MluCI AATT 4 cut(s) 20, 71, 221, 506
MmeI TCCRAC 2 cut(s) 285, 522
MslI CAYNNNNRTG 4 cut(s) 192, 443, 449, 473
MspI CCGG 1 cut(s) 515
MspR9I CCNGG 2 cut(s) 293, 340
MvaI CCWGG 2 cut(s) 293, 340
MwoI GCNNNNNNNGC 2 cut(s) 90, 684
NcoI CCATGG 1 cut(s) 643
NdeI CATATG 1 cut(s) 390
NlaIII CATG 7 cut(s) 54, 62, 247, 448, 478, 647, 709
NmuCI GTSAC 1 cut(s) 8
NspV TTCGAA 1 cut(s) 75
OliI CACNNNNGTG 1 cut(s) 192
PkrI GCNGC 2 cut(s) 138, 669
Ppu21I YACGTR 1 cut(s) 403
PshAI GACNNNNGTC 1 cut(s) 554
Psp6I CCWGG 2 cut(s) 291, 338
PspGI CCWGG 2 cut(s) 291, 338
PspPI GGNCC 1 cut(s) 274
PstI CTGCAG 1 cut(s) 672
RsaI GTAC 4 cut(s) 238, 401, 513, 701
RsaNI GTAC 4 cut(s) 237, 400, 512, 700
RseI CAYNNNNRTG 4 cut(s) 192, 443, 449, 473
SatI GCNGC 2 cut(s) 137, 668
Sau96I GGNCC 1 cut(s) 274
ScaI AGTACT 1 cut(s) 238
ScrFI CCNGG 2 cut(s) 293, 340
SduI GDGCHC 1 cut(s) 632
SetI ASST 8 cut(s) 316, 384, 405, 423, 453, 483, 566, 701
SfaNI GCATC 2 cut(s) 193, 612
SfcI CTRYAG 1 cut(s) 668
SfuI TTCGAA 1 cut(s) 75
SmiMI CAYNNNNRTG 4 cut(s) 192, 443, 449, 473
Sse9I AATT 4 cut(s) 20, 71, 221, 506
SspI AATATT 1 cut(s) 321
SspMI CTAG 2 cut(s) 141, 372
StyD4I CCNGG 2 cut(s) 291, 338
StyI CCWWGG 2 cut(s) 140, 643
TaaI ACNGT 1 cut(s) 236
TaiI ACGT 3 cut(s) 405, 453, 483
TaqI TCGA 3 cut(s) 75, 376, 558
TasI AATT 4 cut(s) 20, 71, 221, 506
TatI WGTACW 1 cut(s) 236
TscAI CASTG 2 cut(s) 445, 475
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 2 cut(s) 136, 667
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 4 cut(s) 47, 84, 269, 377
TspGWI ACGGA 2 cut(s) 27, 362
TspRI CASTG 2 cut(s) 445, 475
XapI RAATTY 1 cut(s) 71
XcmI CCANNNNNNNNNTGG 1 cut(s) 538
XmaJI CCTAGG 1 cut(s) 140
XspI CTAG 2 cut(s) 141, 372
ZrmI AGTACT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.