Prupe.2G094100_v2.0.a1

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000735
Physical Location & Seq
Forward (+)
14870148 .. 14871062
915 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G094100.1

Sequence Viewer

Length: 717 bp
ATGGCCAGTGAGAGTTTGAAAGTGGTGGGCGAGACACATTGCAAGGCATGGCTGCAACAAGCTTTCGAACCCGAAAACGAGTCTTTGCCGTGCACCAAGTTCTTGATGGATGTCAAGGCCACGGTTCGGCGGTGCCTGCTGCAGCAGCCTATTGATTTCAATGAAGATATGTCGTTGTTTTTGGAAGACGATAATTGGAGCGTGGATGAGAATGAAGACACAGTCGAACCCCTAGCTAACAACCCATTTGACTTGGCCAACGCCATATCCCAAACCCTTTCTCGGCTTCAAGTCCCCTCCCACGCTCATCAAGACATGATTGACACGATCATATTGCCCGAAGCTTTTAAGGCCGCAAGGGAGCATTGTGGGGCGCCCAAGGTTCTGCGTATCGCGGTGCGCGTCGATGTTTATGTTGCTGAGAAGAATTACATGGAGGAGGGGTTTGGGGGCACGGATGTTTTGGAGGTTGAGGATGACGGTAGTGATCAGCCTGCATTTGTGCCTGCGAGTAGGGCGTTCATTGAGAAGTTGGAGAAGGCTAGAGTCGAACTCTCGACAATGTGTTCGATTTGCATGGAGGATATTGTAGTTGGTTCCGAGGCGAATCGAATGCCGTGTTCACATTTTTACCATGAGGGTTGCATTGTGGAGTGGCTGCAGAAAAGTAGGTTTTGCCCGTTGTGCCGGTATTCCCTGCCTGCTGATCATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

239

Amino Acids

26.86

Weight (kDa)

4.6

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 132, 373
AccII CGCG 2 cut(s) 395, 402
AciI CCGC 3 cut(s) 130, 354, 395
AcoI YGGCCR 2 cut(s) 3, 255
AcyI GRCGYC 1 cut(s) 374
AfiI CCNNNNNNNGG 2 cut(s) 126, 282
AgsI TTSAA 3 cut(s) 19, 160, 290
AluBI AGCT 3 cut(s) 62, 236, 344
AluI AGCT 3 cut(s) 62, 236, 344
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 26
Alw44I GTGCAC 1 cut(s) 91
AoxI GGCC 4 cut(s) 3, 117, 255, 351
ApaLI GTGCAC 1 cut(s) 91
ApeKI GCWGC 5 cut(s) 52, 139, 142, 145, 658
ArsI GACNNNNNNTTYG 2 cut(s) 550, 582
AspLEI GCGC 2 cut(s) 376, 402
AsuII TTCGAA 1 cut(s) 66
BaeGI GKGCMC 2 cut(s) 95, 455
BalI TGGCCA 2 cut(s) 5, 257
BanI GGYRCC 2 cut(s) 132, 373
BbsI GAAGAC 2 cut(s) 192, 222
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 5 cut(s) 39, 126, 154, 157, 645
BccI CCATC 1 cut(s) 100
BceAI ACGGC 2 cut(s) 73, 601
BcgI CGANNNNNNTGC 4 cut(s) 316, 350, 595, 629
BclI TGATCA 2 cut(s) 487, 706
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 2 cut(s) 233, 543
BfmI CTRYAG 2 cut(s) 140, 659
BfoI RGCGCY 1 cut(s) 377
BisI GCNGC 6 cut(s) 53, 140, 143, 146, 354, 659
BlsI GCNGC 6 cut(s) 54, 141, 144, 147, 355, 660
BmiI GGNNCC 3 cut(s) 134, 375, 598
BpiI GAAGAC 2 cut(s) 192, 222
BplI GAGNNNNNCTC 2 cut(s) 537, 569
Bpu14I TTCGAA 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 120, 378, 600
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 282
Bse118I RCCGGY 1 cut(s) 687
Bse1I ACTGG 1 cut(s) 6
Bse3DI GCAATG 1 cut(s) 37
BseDI CCNNGG 3 cut(s) 120, 378, 600
BseGI GGATG 4 cut(s) 115, 211, 463, 481
BseLI CCNNNNNNNGG 2 cut(s) 126, 282
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 6
BseRI GAGGAG 1 cut(s) 452
BseSI GKGCMC 2 cut(s) 95, 455
BseXI GCAGC 5 cut(s) 39, 126, 154, 157, 645
Bsh1236I CGCG 2 cut(s) 395, 402
BshFI GGCC 4 cut(s) 5, 119, 257, 353
BshNI GGYRCC 2 cut(s) 132, 373
BsiHKAI GWGCWC 1 cut(s) 95
BsiSI CCGG 1 cut(s) 688
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 2 cut(s) 126, 282
BsmAI GTCTC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 618
BsnI GGCC 4 cut(s) 5, 119, 257, 353
Bsp119I TTCGAA 1 cut(s) 66
Bsp1286I GDGCHC 2 cut(s) 95, 455
Bsp143I GATC 3 cut(s) 327, 487, 706
BspACI CCGC 3 cut(s) 130, 354, 395
BspANI GGCC 4 cut(s) 5, 119, 257, 353
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 2 cut(s) 395, 402
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 3 cut(s) 134, 375, 598
BspMAI CTGCAG 2 cut(s) 144, 663
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 2 cut(s) 132, 373
BsrDI GCAATG 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 687
BsrI ACTGG 1 cut(s) 6
BssAI RCCGGY 1 cut(s) 687
BssECI CCNNGG 3 cut(s) 120, 378, 600
BssMI GATC 3 cut(s) 327, 487, 706
BssNI GRCGYC 1 cut(s) 374
BssT1I CCWWGG 1 cut(s) 378
Bst4CI ACNGT 3 cut(s) 124, 223, 482
BstACI GRCGYC 1 cut(s) 374
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 4 cut(s) 137, 495, 507, 702
BstDEI CTNAG 1 cut(s) 420
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 4 cut(s) 115, 211, 463, 481
BstFNI CGCG 2 cut(s) 395, 402
BstH2I RGCGCY 1 cut(s) 377
BstHHI GCGC 2 cut(s) 376, 402
BstKTI GATC 3 cut(s) 330, 490, 709
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 3 cut(s) 327, 487, 706
BstMWI GCNNNNNNNGC 5 cut(s) 136, 145, 350, 515, 684
BstSFI CTRYAG 2 cut(s) 140, 659
BstSLI GKGCMC 2 cut(s) 95, 455
BstUI CGCG 2 cut(s) 395, 402
BstV1I GCAGC 5 cut(s) 39, 126, 154, 157, 645
BstV2I GAAGAC 2 cut(s) 192, 222
BsuRI GGCC 4 cut(s) 5, 119, 257, 353
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 4 cut(s) 115, 211, 463, 481
BtsIMutI CAGTG 1 cut(s) 13
Cac8I GCNNGC 4 cut(s) 137, 495, 507, 702
CciI TCATGA 1 cut(s) 709
CfoI GCGC 2 cut(s) 376, 402
Cfr10I RCCGGY 1 cut(s) 687
CseI GACGC 1 cut(s) 391
CviAII CATG 6 cut(s) 48, 316, 433, 577, 635, 710
DdeI CTNAG 1 cut(s) 420
DinI GGCGCC 1 cut(s) 375
DpnI GATC 3 cut(s) 329, 489, 708
DpnII GATC 3 cut(s) 327, 487, 706
EaeI YGGCCR 2 cut(s) 3, 255
Eco130I CCWWGG 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 378
EgeI GGCGCC 1 cut(s) 375
EheI GGCGCC 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 6 cut(s) 51, 319, 436, 580, 638, 713
FaqI GGGAC 1 cut(s) 278
FatI CATG 6 cut(s) 47, 315, 432, 576, 634, 709
FbaI TGATCA 2 cut(s) 487, 706
Fnu4HI GCNGC 6 cut(s) 53, 140, 143, 146, 354, 659
FokI GGATG 4 cut(s) 122, 218, 470, 488
Fsp4HI GCNGC 6 cut(s) 53, 140, 143, 146, 354, 659
FspBI CTAG 2 cut(s) 233, 543
GlaI GCGC 2 cut(s) 375, 401
GluI GCNGC 6 cut(s) 53, 140, 143, 146, 354, 659
HaeII RGCGCY 1 cut(s) 377
HaeIII GGCC 4 cut(s) 5, 119, 257, 353
HapII CCGG 1 cut(s) 688
HgaI GACGC 1 cut(s) 391
HhaI GCGC 2 cut(s) 376, 402
Hin1I GRCGYC 1 cut(s) 374
Hin1II CATG 6 cut(s) 51, 319, 436, 580, 638, 713
Hin6I GCGC 2 cut(s) 374, 400
HinP1I GCGC 2 cut(s) 374, 400
HindIII AAGCTT 2 cut(s) 60, 342
HinfI GANTC 3 cut(s) 80, 546, 607
HpaII CCGG 1 cut(s) 688
Hpy166II GTNNAC 2 cut(s) 93, 623
Hpy188I TCNGA 1 cut(s) 601
Hpy188III TCNNGA 4 cut(s) 103, 311, 556, 710
Hpy8I GTNNAC 2 cut(s) 93, 623
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 1 cut(s) 532
HpyCH4III ACNGT 3 cut(s) 124, 223, 482
HpyCH4V TGCA 8 cut(s) 42, 55, 93, 142, 497, 576, 645, 661
HpyF10VI GCNNNNNNNGC 5 cut(s) 136, 145, 350, 515, 684
HpyF3I CTNAG 1 cut(s) 420
Hsp92I GRCGYC 1 cut(s) 374
Hsp92II CATG 6 cut(s) 51, 319, 436, 580, 638, 713
HspAI GCGC 2 cut(s) 374, 400
KasI GGCGCC 1 cut(s) 373
Ksp22I TGATCA 2 cut(s) 487, 706
Kzo9I GATC 3 cut(s) 327, 487, 706
LmnI GCTCC 2 cut(s) 198, 361
LpnPI CCDG 6 cut(s) 19, 149, 507, 519, 701, 710
Lsp1109I GCAGC 5 cut(s) 39, 126, 154, 157, 645
MaeI CTAG 2 cut(s) 233, 543
MalI GATC 3 cut(s) 329, 489, 708
MboI GATC 3 cut(s) 327, 487, 706
MboII GAAGA 4 cut(s) 176, 197, 227, 436
MhlI GDGCHC 2 cut(s) 95, 455
MlsI TGGCCA 2 cut(s) 5, 257
MluCI AATT 2 cut(s) 193, 427
MluNI TGGCCA 2 cut(s) 5, 257
Mly113I GGCGCC 1 cut(s) 374
MlyI GAGTC 2 cut(s) 89, 555
MmeI TCCRAC 1 cut(s) 513
MnlI CCTC 8 cut(s) 307, 430, 433, 460, 466, 574, 595, 631
Mox20I TGGCCA 2 cut(s) 5, 257
MscI TGGCCA 2 cut(s) 5, 257
MseI TTAA 1 cut(s) 348
Msp20I TGGCCA 2 cut(s) 5, 257
MspI CCGG 1 cut(s) 688
Mva1269I GAATGC 1 cut(s) 618
MvnI CGCG 2 cut(s) 395, 402
MwoI GCNNNNNNNGC 5 cut(s) 136, 145, 350, 515, 684
NarI GGCGCC 1 cut(s) 374
NdeII GATC 3 cut(s) 327, 487, 706
NlaIII CATG 6 cut(s) 51, 319, 436, 580, 638, 713
NlaIV GGNNCC 3 cut(s) 134, 375, 598
NmeAIII GCCGAG 1 cut(s) 262
NspV TTCGAA 1 cut(s) 66
PagI TCATGA 1 cut(s) 709
PcsI WCGNNNNNNNCGW 1 cut(s) 399
PctI GAATGC 1 cut(s) 618
PfeI GAWTC 1 cut(s) 607
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 6 cut(s) 54, 141, 144, 147, 355, 660
PleI GAGTC 2 cut(s) 88, 554
PluTI GGCGCC 1 cut(s) 377
PpsI GAGTC 2 cut(s) 88, 554
PspN4I GGNNCC 3 cut(s) 134, 375, 598
PstI CTGCAG 2 cut(s) 144, 663
PsyI GACNNNGTC 1 cut(s) 221
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 6 cut(s) 53, 140, 143, 146, 354, 659
Sau3AI GATC 3 cut(s) 327, 487, 706
SchI GAGTC 2 cut(s) 89, 555
SduI GDGCHC 2 cut(s) 95, 455
SetI ASST 6 cut(s) 64, 238, 346, 384, 471, 674
SfcI CTRYAG 2 cut(s) 140, 659
SfoI GGCGCC 1 cut(s) 375
SfuI TTCGAA 1 cut(s) 66
Sse9I AATT 2 cut(s) 193, 427
SsiI CCGC 3 cut(s) 130, 354, 395
SspDI GGCGCC 1 cut(s) 373
SspMI CTAG 2 cut(s) 233, 543
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 3 cut(s) 124, 223, 482
TaqI TCGA 7 cut(s) 66, 225, 405, 549, 557, 569, 610
TasI AATT 2 cut(s) 193, 427
TauI GCSGC 1 cut(s) 356
TfiI GAWTC 1 cut(s) 607
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 1 cut(s) 13
TseI GCWGC 5 cut(s) 52, 139, 142, 145, 658
TspDTI ATGAA 3 cut(s) 177, 228, 511
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 1 cut(s) 13
Tth111I GACNNNGTC 1 cut(s) 221
VneI GTGCAC 1 cut(s) 91
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XspI CTAG 2 cut(s) 233, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.