RLG00000035225

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000735
Physical Location & Seq
Reverse (-)
63181615 .. 63182449
835 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035225

Sequence Viewer

Length: 507 bp
ATGGCTACTGAAAATTGGCAAGTGGTGGGTGACATCACCTGCAACCCAAAATACCTAGGTCTTGTTCAACCTGAAAAGGAGCTGCCTAACACCAAATTCTTGATGGATTTCAATGTCAAGATGAGGCGCGCTCGGTCTACTGTTTTGGAGGATGATGAAGAAATTGTAGAAGAGAAGAATCTGCGCTCAGTCGAACTTGAGTACAATCCACATAGCATGTGGGTTGCCATAAATAAAGAGCTTCAAGACCTGCAAGTTCCAATCCAAGTTCGTGCAGCCATTGAAGATCCAATACTCAAGGGAACCAGAGTCGCACGAGCCAATAGCTCTTATGACGCTCGGAAGTATCTGCATATAGAGGTGGCTATCGATTTTCTTGTTGGCGTTTTTGATGGTATTGATGAATATGAGCCTACGTTTGTGCCGGCAAGTGAAGCGTCCATTAAGAAACTGAAGAAGACAAGAGCCGAAGTGTCGTCGACAATGTGCACGGTACTGCTACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

169

Amino Acids

19.2

Weight (kDa)

5.19

Isoelectric Point (pI)

53.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 47
Acc36I ACCTGC 2 cut(s) 47, 258
AccI GTMKAC 2 cut(s) 137, 479
AccII CGCG 1 cut(s) 129
AclWI GGATC 1 cut(s) 281
AcsI RAATTY 1 cut(s) 95
AcuI CTGAAG 1 cut(s) 473
AfaI GTAC 2 cut(s) 203, 495
AgsI TTSAA 4 cut(s) 68, 112, 245, 284
AluBI AGCT 3 cut(s) 82, 241, 327
AluI AGCT 3 cut(s) 82, 241, 327
Alw21I GWGCWC 1 cut(s) 491
Alw44I GTGCAC 1 cut(s) 487
AlwI GGATC 1 cut(s) 281
ApaLI GTGCAC 1 cut(s) 487
ApeKI GCWGC 2 cut(s) 82, 275
ApoI RAATTY 1 cut(s) 95
AspA2I CCTAGG 1 cut(s) 55
AspLEI GCGC 3 cut(s) 129, 131, 186
AsuHPI GGTGA 2 cut(s) 28, 41
AvrII CCTAGG 1 cut(s) 55
BaeGI GKGCMC 1 cut(s) 491
BarI GAAGNNNNNNTAC 2 cut(s) 276, 308
BauI CACGAG 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 464
Bbv12I GWGCWC 1 cut(s) 491
BbvI GCAGC 2 cut(s) 69, 287
BccI CCATC 2 cut(s) 97, 386
BfaI CTAG 1 cut(s) 56
BfuAI ACCTGC 2 cut(s) 47, 258
BisI GCNGC 2 cut(s) 83, 276
BlnI CCTAGG 1 cut(s) 55
BlsI GCNGC 2 cut(s) 84, 277
BmiI GGNNCC 1 cut(s) 304
BpiI GAAGAC 1 cut(s) 464
BplI GAGNNNNNCTC 2 cut(s) 115, 147
BpuEI CTTGAG 2 cut(s) 218, 281
Bsa29I ATCGAT 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 55
Bse118I RCCGGY 1 cut(s) 424
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 1 cut(s) 55
BseGI GGATG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 201
BsePI GCGCGC 1 cut(s) 127
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 2 cut(s) 69, 287
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 129
BshVI ATCGAT 1 cut(s) 369
BsiHKAI GWGCWC 1 cut(s) 491
BsiSI CCGG 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 1 cut(s) 286
BspCNI CTCAG 1 cut(s) 200
BspDI ATCGAT 1 cut(s) 369
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 1 cut(s) 304
BspMI ACCTGC 2 cut(s) 47, 258
BspPI GGATC 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 55
BssHII GCGCGC 1 cut(s) 127
BssMI GATC 1 cut(s) 286
BssSI CACGAG 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 55
Bst2BI CACGAG 1 cut(s) 315
Bst4CI ACNGT 2 cut(s) 142, 493
Bst6I CTCTTC 1 cut(s) 165
BstC8I GCNNGC 2 cut(s) 129, 426
BstDEI CTNAG 1 cut(s) 187
BstF5I GGATG 1 cut(s) 157
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 3 cut(s) 129, 131, 186
BstKTI GATC 1 cut(s) 289
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNSI RCATGY 1 cut(s) 220
BstSLI GKGCMC 1 cut(s) 491
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 69, 287
BstV2I GAAGAC 1 cut(s) 464
BstX2I RGATCY 1 cut(s) 286
BstYI RGATCY 1 cut(s) 286
Bsu15I ATCGAT 1 cut(s) 369
BsuTUI ATCGAT 1 cut(s) 369
BtsCI GGATG 1 cut(s) 157
BveI ACCTGC 2 cut(s) 47, 258
Cac8I GCNNGC 2 cut(s) 129, 426
CfoI GCGC 3 cut(s) 129, 131, 186
Cfr10I RCCGGY 1 cut(s) 424
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 2 cut(s) 344, 426
Csp6I GTAC 2 cut(s) 202, 494
CviAII CATG 1 cut(s) 217
CviJI RGCY 9 cut(s) 5, 82, 241, 278, 320, 327, 365, 412, 467
CviKI_1 RGCY 9 cut(s) 5, 82, 241, 278, 320, 327, 365, 412, 467
CviQI GTAC 2 cut(s) 202, 494
DdeI CTNAG 1 cut(s) 187
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 473
EcoT14I CCWWGG 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 55
FaeI CATG 1 cut(s) 220
FaiI YATR 7 cut(s) 213, 218, 230, 333, 354, 356, 408
FatI CATG 1 cut(s) 216
FblI GTMKAC 2 cut(s) 137, 479
Fnu4HI GCNGC 2 cut(s) 83, 276
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 2 cut(s) 83, 276
FspBI CTAG 1 cut(s) 56
GlaI GCGC 3 cut(s) 128, 130, 185
GluI GCNGC 2 cut(s) 83, 276
HapII CCGG 1 cut(s) 425
HgaI GACGC 2 cut(s) 344, 426
HhaI GCGC 3 cut(s) 129, 131, 186
Hin1II CATG 1 cut(s) 220
Hin6I GCGC 3 cut(s) 127, 129, 184
HinP1I GCGC 3 cut(s) 127, 129, 184
HincII GTYRAC 1 cut(s) 480
HindII GTYRAC 1 cut(s) 480
HinfI GANTC 2 cut(s) 178, 309
HpaII CCGG 1 cut(s) 425
HphI GGTGA 2 cut(s) 28, 41
Hpy166II GTNNAC 3 cut(s) 138, 480, 489
Hpy188I TCNGA 1 cut(s) 342
Hpy188III TCNNGA 3 cut(s) 100, 118, 245
Hpy8I GTNNAC 3 cut(s) 138, 480, 489
Hpy99I CGWCG 1 cut(s) 481
HpyCH4III ACNGT 2 cut(s) 142, 493
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 5 cut(s) 42, 253, 275, 352, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpyF3I CTNAG 1 cut(s) 187
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 1 cut(s) 220
HspAI GCGC 3 cut(s) 127, 129, 184
KroI GCCGGC 1 cut(s) 424
KroNI GCCGGC 1 cut(s) 426
Kzo9I GATC 1 cut(s) 286
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 5 cut(s) 52, 84, 263, 319, 438
Lsp1109I GCAGC 2 cut(s) 69, 287
MaeI CTAG 1 cut(s) 56
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 1 cut(s) 29
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 6 cut(s) 170, 182, 187, 296, 466, 469
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 3 cut(s) 13, 95, 162
MlyI GAGTC 1 cut(s) 318
MnlI CCTC 3 cut(s) 117, 142, 352
MroNI GCCGGC 1 cut(s) 424
MseI TTAA 1 cut(s) 444
MspI CCGG 1 cut(s) 425
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 1 cut(s) 434
NaeI GCCGGC 1 cut(s) 426
NdeII GATC 1 cut(s) 286
NgoMIV GCCGGC 1 cut(s) 424
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 1 cut(s) 29
NspI RCATGY 1 cut(s) 220
PaqCI CACCTGC 1 cut(s) 47
PauI GCGCGC 1 cut(s) 127
PdiI GCCGGC 1 cut(s) 426
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 2 cut(s) 84, 277
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
PspN4I GGNNCC 1 cut(s) 304
PsuI RGATCY 1 cut(s) 286
PteI GCGCGC 1 cut(s) 127
RsaI GTAC 2 cut(s) 203, 495
RsaNI GTAC 2 cut(s) 202, 494
SalI GTCGAC 1 cut(s) 478
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 2 cut(s) 83, 276
Sau3AI GATC 1 cut(s) 286
SchI GAGTC 1 cut(s) 318
SduI GDGCHC 1 cut(s) 491
SmlI CTYRAG 2 cut(s) 197, 296
SmoI CTYRAG 2 cut(s) 197, 296
Sse9I AATT 3 cut(s) 13, 95, 162
SspMI CTAG 1 cut(s) 56
StyI CCWWGG 1 cut(s) 55
TaaI ACNGT 2 cut(s) 142, 493
TaiI ACGT 1 cut(s) 419
TaqI TCGA 3 cut(s) 192, 369, 479
TaqII GACCGA 1 cut(s) 123
TasI AATT 3 cut(s) 13, 95, 162
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 2 cut(s) 82, 275
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 2 cut(s) 171, 417
VneI GTGCAC 1 cut(s) 487
XapI RAATTY 1 cut(s) 95
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 2 cut(s) 100, 216
XmaJI CCTAGG 1 cut(s) 55
XmiI GTMKAC 2 cut(s) 137, 479
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.