Rw2G047210

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000735
Physical Location & Seq
Reverse (-)
74576839 .. 74577537
699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G047210.1

Sequence Viewer

Length: 699 bp
ATGGCCATGGCCATGGCCAGTGACTACTGGGAAGTGGTGGGCGACACTTTCTGCGACATACTTGTCGCCTTCATGGAACCAGAAAACGAGTTTCTATGCAGCAGCACCAAGTTCTTGATGGAATTTGAGGCGGTGATCAGACGTGTCACCTCCGACGAGGCGGACCGGCTCCTAGACTACGACGTCATCCCCCGCACGATCTGGAAGACCACCTCAATCGAACTTGACGACTCTAACTCATCATCCATGGATAGAGCTCTATCTGAAACACTTCGGCAGCTGCAAGTCCCACTGGAGGAGCGTAGCTCCATGGTGGAGAAAGTATTGTTCAAAGCCTTCGAGGCGGCACAGAAACCTAGTCCTTACACCGGTGGGAAGAGACTGCGTATGCGGGTGAACATCGATATGTATGTGCTTGATGACGATGTTGATGAGGACGCAAATGATGGTGGTGGTGGCATTATTGGTGATTATTATGAGCCTAGGTTTATGCCGGCCAGTAAATTGTCGGTTGAGAAACTGGAGAGGACGAGTGTCCAAGTGCCATCAACAGTGTGTTCGGTTTGTATGGAGGAGATTAGTGTTGGTTTCGAAGCAACTAAAATGCCTTGTTCACATCTTTACCATGAGTGCTGCATTGTAGAGTGGCTGCAGAAAAGTGGGGTTTGCCCTTTGTGTAAGTTCCGCATGCCCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

232

Amino Acids

26.32

Weight (kDa)

4.55

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 185 - 227 7.2e-12 Ring finger domain
zf-C3HC4_2 PF13923 186 - 226 3.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 186 - 213 1.2e-06 RING-like zinc finger
zf-C3HC4 PF00097 186 - 226 7.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 197 - 227 6.8e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 62, 182
AatII GACGTC 1 cut(s) 186
AciI CCGC 6 cut(s) 131, 161, 193, 344, 391, 685
AcoI YGGCCR 4 cut(s) 3, 9, 15, 495
AcsI RAATTY 1 cut(s) 122
AcyI GRCGYC 1 cut(s) 183
AfiI CCNNNNNNNGG 2 cut(s) 295, 368
AflIII ACRYGT 1 cut(s) 142
AgeI ACCGGT 1 cut(s) 368
AgsI TTSAA 1 cut(s) 331
AjiI CACGTC 1 cut(s) 143
AluBI AGCT 3 cut(s) 257, 280, 306
AluI AGCT 3 cut(s) 257, 280, 306
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 1 cut(s) 373
AoxI GGCC 4 cut(s) 3, 9, 15, 495
ApeKI GCWGC 6 cut(s) 99, 102, 277, 280, 633, 649
ApoI RAATTY 1 cut(s) 122
AsiGI ACCGGT 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 270
AspA2I CCTAGG 1 cut(s) 482
AspS9I GGNCC 1 cut(s) 163
AsuHPI GGTGA 4 cut(s) 139, 145, 406, 479
AsuII TTCGAA 1 cut(s) 591
AvaII GGWCC 1 cut(s) 163
AvrII CCTAGG 1 cut(s) 482
BalI TGGCCA 3 cut(s) 5, 11, 17
BanII GRGCYC 1 cut(s) 259
BbsI GAAGAC 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 259
BbvI GCAGC 6 cut(s) 111, 114, 267, 289, 620, 636
BccI CCATC 3 cut(s) 112, 440, 553
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 373
BfaI CTAG 3 cut(s) 173, 357, 483
BfmI CTRYAG 1 cut(s) 650
BglI GCCNNNNNGGC 1 cut(s) 341
BisI GCNGC 7 cut(s) 100, 103, 278, 281, 345, 634, 650
BlnI CCTAGG 1 cut(s) 482
BlsI GCNGC 7 cut(s) 101, 104, 279, 282, 346, 635, 651
Bme18I GGWCC 1 cut(s) 163
BmgBI CACGTC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 2 cut(s) 78, 170
BmrI ACTGGG 1 cut(s) 37
BmuI ACTGGG 1 cut(s) 37
BoxI GACNNNNGTC 1 cut(s) 533
BpiI GAAGAC 1 cut(s) 212
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 2 cut(s) 314, 542
Bpu14I TTCGAA 1 cut(s) 591
Bsa29I ATCGAT 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 5 cut(s) 6, 12, 246, 309, 482
BsaWI WCCGGW 1 cut(s) 368
Bsc4I CCNNNNNNNGG 2 cut(s) 295, 368
Bse118I RCCGGY 3 cut(s) 165, 368, 493
Bse1I ACTGG 5 cut(s) 18, 32, 297, 498, 525
BseCI ATCGAT 1 cut(s) 402
BseDI CCNNGG 5 cut(s) 6, 12, 246, 309, 482
BseGI GGATG 2 cut(s) 186, 242
BseLI CCNNNNNNNGG 2 cut(s) 295, 368
BseNI ACTGG 5 cut(s) 18, 32, 297, 498, 525
BseRI GAGGAG 2 cut(s) 311, 587
BseXI GCAGC 6 cut(s) 111, 114, 267, 289, 620, 636
BshFI GGCC 4 cut(s) 5, 11, 17, 497
BshTI ACCGGT 1 cut(s) 368
BshVI ATCGAT 1 cut(s) 402
BsiHKAI GWGCWC 1 cut(s) 259
BsiSI CCGG 3 cut(s) 166, 369, 494
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 2 cut(s) 295, 368
BsmAI GTCTC 1 cut(s) 373
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 4 cut(s) 5, 11, 17, 497
Bsp119I TTCGAA 1 cut(s) 591
Bsp1286I GDGCHC 1 cut(s) 259
Bsp143I GATC 2 cut(s) 135, 198
Bsp19I CCATGG 4 cut(s) 6, 12, 246, 309
BspACI CCGC 6 cut(s) 131, 161, 193, 344, 391, 685
BspANI GGCC 4 cut(s) 5, 11, 17, 497
BspDI ATCGAT 1 cut(s) 402
BspLI GGNNCC 2 cut(s) 78, 170
BspMAI CTGCAG 1 cut(s) 654
BspT104I TTCGAA 1 cut(s) 591
BsrFI RCCGGY 3 cut(s) 165, 368, 493
BsrI ACTGG 5 cut(s) 18, 32, 297, 498, 525
BssAI RCCGGY 3 cut(s) 165, 368, 493
BssECI CCNNGG 5 cut(s) 6, 12, 246, 309, 482
BssMI GATC 2 cut(s) 135, 198
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 5 cut(s) 6, 12, 246, 309, 482
Bst4CI ACNGT 1 cut(s) 553
Bst6I CTCTTC 1 cut(s) 371
BstACI GRCGYC 1 cut(s) 183
BstBI TTCGAA 1 cut(s) 591
BstC8I GCNNGC 2 cut(s) 495, 689
BstDSI CCRYGG 4 cut(s) 6, 12, 246, 309
BstF5I GGATG 2 cut(s) 186, 242
BstKTI GATC 2 cut(s) 138, 201
BstMAI GTCTC 1 cut(s) 373
BstMBI GATC 2 cut(s) 135, 198
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNSI RCATGY 1 cut(s) 691
BstPAI GACNNNNGTC 1 cut(s) 533
BstSFI CTRYAG 1 cut(s) 650
BstV1I GCAGC 6 cut(s) 111, 114, 267, 289, 620, 636
BstV2I GAAGAC 1 cut(s) 212
BstXI CCANNNNNNTGG 1 cut(s) 13
Bsu15I ATCGAT 1 cut(s) 402
BsuRI GGCC 4 cut(s) 5, 11, 17, 497
BsuTUI ATCGAT 1 cut(s) 402
BtgI CCRYGG 4 cut(s) 6, 12, 246, 309
BtrI CACGTC 1 cut(s) 143
BtsCI GGATG 2 cut(s) 186, 242
BtsIMutI CAGTG 3 cut(s) 25, 290, 558
Cac8I GCNNGC 2 cut(s) 495, 689
Cfr10I RCCGGY 3 cut(s) 165, 368, 493
Cfr13I GGNCC 1 cut(s) 163
ClaI ATCGAT 1 cut(s) 402
CpoI CGGWCCG 1 cut(s) 163
CseI GACGC 1 cut(s) 446
CspAI ACCGGT 1 cut(s) 368
CspI CGGWCCG 1 cut(s) 163
CviAII CATG 7 cut(s) 7, 13, 73, 247, 310, 626, 688
DpnI GATC 2 cut(s) 137, 200
DpnII GATC 2 cut(s) 135, 198
DrdI GACNNNNNNGTC 2 cut(s) 62, 182
DseDI GACNNNNNNGTC 2 cut(s) 62, 182
EaeI YGGCCR 4 cut(s) 3, 9, 15, 495
Eam1104I CTCTTC 1 cut(s) 371
EarI CTCTTC 1 cut(s) 371
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 257
Eco130I CCWWGG 5 cut(s) 6, 12, 246, 309, 482
Eco24I GRGCYC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 257
EcoT14I CCWWGG 5 cut(s) 6, 12, 246, 309, 482
EcoT38I GRGCYC 1 cut(s) 259
ErhI CCWWGG 5 cut(s) 6, 12, 246, 309, 482
FaeI CATG 7 cut(s) 10, 16, 76, 250, 313, 629, 691
FaqI GGGAC 1 cut(s) 272
FatI CATG 7 cut(s) 6, 12, 72, 246, 309, 625, 687
FauI CCCGC 2 cut(s) 200, 384
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 7 cut(s) 100, 103, 278, 281, 345, 634, 650
FokI GGATG 2 cut(s) 173, 229
FriOI GRGCYC 1 cut(s) 259
Fsp4HI GCNGC 7 cut(s) 100, 103, 278, 281, 345, 634, 650
FspBI CTAG 3 cut(s) 173, 357, 483
GluI GCNGC 7 cut(s) 100, 103, 278, 281, 345, 634, 650
GsuI CTGGAG 2 cut(s) 314, 542
HaeIII GGCC 4 cut(s) 5, 11, 17, 497
HapII CCGG 3 cut(s) 166, 369, 494
HgaI GACGC 1 cut(s) 446
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 7 cut(s) 10, 16, 76, 250, 313, 629, 691
HinfI GANTC 1 cut(s) 230
HpaII CCGG 3 cut(s) 166, 369, 494
HphI GGTGA 4 cut(s) 139, 145, 406, 479
Hpy166II GTNNAC 2 cut(s) 397, 614
Hpy188I TCNGA 3 cut(s) 140, 154, 265
Hpy188III TCNNGA 2 cut(s) 115, 202
Hpy8I GTNNAC 2 cut(s) 397, 614
Hpy99I CGWCG 2 cut(s) 158, 185
HpyAV CCTTC 2 cut(s) 79, 346
HpyCH4III ACNGT 1 cut(s) 553
HpyCH4IV ACGT 2 cut(s) 142, 183
HpyCH4V TGCA 4 cut(s) 99, 283, 636, 652
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpySE526I ACGT 2 cut(s) 142, 183
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 7 cut(s) 10, 16, 76, 250, 313, 629, 691
KroI GCCGGC 1 cut(s) 493
KroNI GCCGGC 1 cut(s) 495
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 2 cut(s) 135, 198
LmnI GCTCC 3 cut(s) 174, 298, 311
Lsp1109I GCAGC 6 cut(s) 111, 114, 267, 289, 620, 636
MaeI CTAG 3 cut(s) 173, 357, 483
MaeII ACGT 2 cut(s) 142, 183
MaeIII GTNAC 2 cut(s) 20, 145
MalI GATC 2 cut(s) 137, 200
MboI GATC 2 cut(s) 135, 198
MboII GAAGA 2 cut(s) 217, 388
MhlI GDGCHC 1 cut(s) 259
MlsI TGGCCA 3 cut(s) 5, 11, 17
MluCI AATT 3 cut(s) 122, 503, 694
MluNI TGGCCA 3 cut(s) 5, 11, 17
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 9 cut(s) 121, 151, 160, 223, 289, 334, 427, 519, 565
Mox20I TGGCCA 3 cut(s) 5, 11, 17
MroNI GCCGGC 1 cut(s) 493
MroXI GAANNNNTTC 1 cut(s) 270
MscI TGGCCA 3 cut(s) 5, 11, 17
MslI CAYNNNNRTG 2 cut(s) 11, 404
Msp20I TGGCCA 3 cut(s) 5, 11, 17
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 3 cut(s) 166, 369, 494
MwoI GCNNNNNNNGC 1 cut(s) 341
NaeI GCCGGC 1 cut(s) 495
NcoI CCATGG 4 cut(s) 6, 12, 246, 309
NdeII GATC 2 cut(s) 135, 198
NgoMIV GCCGGC 1 cut(s) 493
NlaIII CATG 7 cut(s) 10, 16, 76, 250, 313, 629, 691
NlaIV GGNNCC 2 cut(s) 78, 170
NmuCI GTSAC 2 cut(s) 20, 145
NspI RCATGY 1 cut(s) 691
NspV TTCGAA 1 cut(s) 591
PaeI GCATGC 1 cut(s) 691
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PdiI GCCGGC 1 cut(s) 495
PdmI GAANNNNTTC 1 cut(s) 270
PinAI ACCGGT 1 cut(s) 368
PkrI GCNGC 7 cut(s) 101, 104, 279, 282, 346, 635, 651
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
PshAI GACNNNNGTC 1 cut(s) 533
Psp124BI GAGCTC 1 cut(s) 259
PspN4I GGNNCC 2 cut(s) 78, 170
PspPI GGNCC 1 cut(s) 163
PstI CTGCAG 1 cut(s) 654
PvuII CAGCTG 1 cut(s) 280
RseI CAYNNNNRTG 2 cut(s) 11, 404
Rsr2I CGGWCCG 1 cut(s) 163
RsrII CGGWCCG 1 cut(s) 163
SacI GAGCTC 1 cut(s) 259
SatI GCNGC 7 cut(s) 100, 103, 278, 281, 345, 634, 650
Sau3AI GATC 2 cut(s) 135, 198
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 1 cut(s) 224
SduI GDGCHC 1 cut(s) 259
SetI ASST 9 cut(s) 145, 152, 186, 215, 259, 282, 308, 358, 488
SfcI CTRYAG 1 cut(s) 650
SfuI TTCGAA 1 cut(s) 591
SgrAI CRCCGGYG 1 cut(s) 368
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 2 cut(s) 11, 404
SphI GCATGC 1 cut(s) 691
Sse9I AATT 3 cut(s) 122, 503, 694
SsiI CCGC 6 cut(s) 131, 161, 193, 344, 391, 685
SspMI CTAG 3 cut(s) 173, 357, 483
SstI GAGCTC 1 cut(s) 259
StyI CCWWGG 5 cut(s) 6, 12, 246, 309, 482
TaaI ACNGT 1 cut(s) 553
TaiI ACGT 2 cut(s) 145, 186
TaqI TCGA 4 cut(s) 219, 339, 402, 591
TasI AATT 3 cut(s) 122, 503, 694
TauI GCSGC 1 cut(s) 347
TscAI CASTG 3 cut(s) 25, 297, 558
TseFI GTSAC 2 cut(s) 20, 145
TseI GCWGC 6 cut(s) 99, 102, 277, 280, 633, 649
Tsp45I GTSAC 2 cut(s) 20, 145
TspDTI ATGAA 1 cut(s) 61
TspRI CASTG 3 cut(s) 25, 297, 558
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 1 cut(s) 122
XceI RCATGY 1 cut(s) 691
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XmaJI CCTAGG 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 3 cut(s) 173, 357, 483
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.