Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1736 |
| AatII | GACGTC | 1 cut(s) | 169 |
| Acc36I | ACCTGC | 1 cut(s) | 639 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 761 |
| AccBSI | CCGCTC | 1 cut(s) | 346 |
| AccI | GTMKAC | 1 cut(s) | 992 |
| AccII | CGCG | 2 cut(s) | 172, 386 |
| AciI | CCGC | 4 cut(s) | 126, 170, 344, 1041 |
| AclWI | GGATC | 4 cut(s) | 877, 1169, 1182, 1547 |
| AcoI | YGGCCR | 3 cut(s) | 752, 1182, 1459 |
| AcsI | RAATTY | 4 cut(s) | 12, 57, 509, 533 |
| AcuI | CTGAAG | 2 cut(s) | 216, 1220 |
| AcyI | GRCGYC | 1 cut(s) | 166 |
| AdeI | CACNNNGTG | 1 cut(s) | 907 |
| AfaI | GTAC | 4 cut(s) | 1076, 1490, 1602, 1845 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 122, 334, 761, 802 |
| AgsI | TTSAA | 9 cut(s) | 265, 352, 668, 743, 1214, 1316, 1334, 1631, 1759 |
| AhlI | ACTAGT | 1 cut(s) | 209 |
| AjnI | CCWGG | 4 cut(s) | 120, 1066, 1715, 1876 |
| AleI | CACNNNNGTG | 1 cut(s) | 212 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 151, 183 |
| Alw21I | GWGCWC | 1 cut(s) | 856 |
| Alw26I | GTCTC | 1 cut(s) | 476 |
| AlwI | GGATC | 4 cut(s) | 877, 1169, 1182, 1547 |
| Ama87I | CYCGRG | 1 cut(s) | 1017 |
| AoxI | GGCC | 7 cut(s) | 588, 728, 752, 1182, 1363, 1394, 1459 |
| ApeKI | GCWGC | 7 cut(s) | 7, 153, 289, 929, 1097, 1189, 1469 |
| ApoI | RAATTY | 4 cut(s) | 12, 57, 509, 533 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1208, 1240 |
| AseI | ATTAAT | 1 cut(s) | 1032 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1865 |
| AspA2I | CCTAGG | 1 cut(s) | 146 |
| AspLEI | GCGC | 3 cut(s) | 153, 174, 1119 |
| AspS9I | GGNCC | 2 cut(s) | 184, 672 |
| AsuHPI | GGTGA | 5 cut(s) | 776, 1056, 1282, 1668, 1709 |
| AvaI | CYCGRG | 1 cut(s) | 1017 |
| AvaII | GGWCC | 2 cut(s) | 184, 672 |
| AvrII | CCTAGG | 1 cut(s) | 146 |
| BalI | TGGCCA | 1 cut(s) | 1461 |
| BamHI | GGATCC | 1 cut(s) | 1174 |
| BbsI | GAAGAC | 3 cut(s) | 781, 1221, 1377 |
| Bbv12I | GWGCWC | 1 cut(s) | 856 |
| BbvCI | CCTCAGC | 1 cut(s) | 306 |
| BbvI | GCAGC | 6 cut(s) | 140, 276, 941, 1084, 1201, 1481 |
| BccI | CCATC | 5 cut(s) | 963, 968, 1564, 1639, 1720 |
| BceAI | ACGGC | 1 cut(s) | 1197 |
| BciT130I | CCWGG | 4 cut(s) | 122, 1068, 1717, 1878 |
| BciVI | GTATCC | 1 cut(s) | 1496 |
| BcoDI | GTCTC | 1 cut(s) | 476 |
| BcuI | ACTAGT | 1 cut(s) | 209 |
| BfmI | CTRYAG | 2 cut(s) | 154, 964 |
| BfoI | RGCGCY | 2 cut(s) | 154, 1120 |
| BfuAI | ACCTGC | 1 cut(s) | 639 |
| BfuI | GTATCC | 1 cut(s) | 1496 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1393 |
| BglII | AGATCT | 2 cut(s) | 466, 1144 |
| BisI | GCNGC | 8 cut(s) | 8, 127, 154, 290, 930, 1098, 1190, 1470 |
| BlnI | CCTAGG | 1 cut(s) | 146 |
| BlsI | GCNGC | 8 cut(s) | 9, 128, 155, 291, 931, 1099, 1191, 1471 |
| BmcAI | AGTACT | 2 cut(s) | 1076, 1602 |
| Bme1390I | CCNGG | 4 cut(s) | 122, 1068, 1717, 1878 |
| Bme18I | GGWCC | 2 cut(s) | 184, 672 |
| BmeT110I | CYCGRG | 1 cut(s) | 1017 |
| BmgT120I | GGNCC | 2 cut(s) | 184, 672 |
| BmiI | GGNNCC | 4 cut(s) | 186, 673, 1176, 1710 |
| BmrFI | CCNGG | 4 cut(s) | 122, 1068, 1717, 1878 |
| BmsI | GCATC | 4 cut(s) | 390, 796, 1243, 1732 |
| BpiI | GAAGAC | 3 cut(s) | 781, 1221, 1377 |
| BpmI | CTGGAG | 1 cut(s) | 471 |
| Bpu10I | CCTNAGC | 1 cut(s) | 306 |
| BsaHI | GRCGYC | 1 cut(s) | 166 |
| BsaJI | CCNNGG | 6 cut(s) | 121, 146, 431, 591, 1388, 1877 |
| BsaWI | WCCGGW | 1 cut(s) | 327 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1824, 1854 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 122, 334, 761, 802 |
| Bse1I | ACTGG | 4 cut(s) | 116, 766, 817, 893 |
| Bse3DI | GCAATG | 2 cut(s) | 296, 498 |
| BseBI | CCWGG | 4 cut(s) | 122, 1068, 1717, 1878 |
| BseDI | CCNNGG | 6 cut(s) | 121, 146, 431, 591, 1388, 1877 |
| BseGI | GGATG | 8 cut(s) | 40, 792, 811, 955, 1258, 1556, 1712, 1747 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 122, 334, 761, 802 |
| BseMI | GCAATG | 2 cut(s) | 296, 498 |
| BseMII | CTCAG | 4 cut(s) | 320, 702, 716, 1808 |
| BseNI | ACTGG | 4 cut(s) | 116, 766, 817, 893 |
| BseRI | GAGGAG | 3 cut(s) | 260, 1340, 1700 |
| BseXI | GCAGC | 6 cut(s) | 140, 276, 941, 1084, 1201, 1481 |
| BseYI | CCCAGC | 1 cut(s) | 801 |
| BsgI | GTGCAG | 1 cut(s) | 275 |
| Bsh1236I | CGCG | 2 cut(s) | 172, 386 |
| BshFI | GGCC | 7 cut(s) | 590, 730, 754, 1184, 1365, 1396, 1461 |
| BsiHKAI | GWGCWC | 1 cut(s) | 856 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1017 |
| BsiSI | CCGG | 1 cut(s) | 328 |
| BslFI | GGGAC | 2 cut(s) | 685, 1883 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 122, 334, 761, 802 |
| BsmAI | GTCTC | 1 cut(s) | 476 |
| BsmFI | GGGAC | 2 cut(s) | 685, 1883 |
| BsmI | GAATGC | 2 cut(s) | 1745, 1807 |
| BsnI | GGCC | 7 cut(s) | 590, 730, 754, 1184, 1365, 1396, 1461 |
| BsoBI | CYCGRG | 1 cut(s) | 1017 |
| Bsp1286I | GDGCHC | 1 cut(s) | 856 |
| Bsp143I | GATC | 6 cut(s) | 82, 466, 882, 1144, 1174, 1552 |
| Bsp19I | CCATGG | 1 cut(s) | 1388 |
| BspACI | CCGC | 4 cut(s) | 126, 170, 344, 1041 |
| BspANI | GGCC | 7 cut(s) | 590, 730, 754, 1184, 1365, 1396, 1461 |
| BspCNI | CTCAG | 4 cut(s) | 319, 703, 717, 1809 |
| BspFNI | CGCG | 2 cut(s) | 172, 386 |
| BspHI | TCATGA | 2 cut(s) | 625, 856 |
| BspLI | GGNNCC | 4 cut(s) | 186, 673, 1176, 1710 |
| BspMAI | CTGCAG | 1 cut(s) | 158 |
| BspMI | ACCTGC | 1 cut(s) | 639 |
| BspPI | GGATC | 4 cut(s) | 877, 1169, 1182, 1547 |
| BspQI | GCTCTTC | 1 cut(s) | 353 |
| BsrBI | CCGCTC | 1 cut(s) | 346 |
| BsrDI | GCAATG | 2 cut(s) | 296, 498 |
| BsrI | ACTGG | 4 cut(s) | 116, 766, 817, 893 |
| BssECI | CCNNGG | 6 cut(s) | 121, 146, 431, 591, 1388, 1877 |
| BssMI | GATC | 6 cut(s) | 82, 466, 882, 1144, 1174, 1552 |
| BssNAI | GTATAC | 1 cut(s) | 993 |
| BssNI | GRCGYC | 1 cut(s) | 166 |
| BssT1I | CCWWGG | 4 cut(s) | 146, 431, 591, 1388 |
| Bst1107I | GTATAC | 1 cut(s) | 993 |
| Bst2UI | CCWGG | 4 cut(s) | 122, 1068, 1717, 1878 |
| Bst4CI | ACNGT | 6 cut(s) | 503, 905, 1238, 1600, 1642, 1766 |
| Bst6I | CTCTTC | 1 cut(s) | 353 |
| BstACI | GRCGYC | 1 cut(s) | 166 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 298 |
| BstC8I | GCNNGC | 4 cut(s) | 294, 834, 1039, 1140 |
| BstDEI | CTNAG | 6 cut(s) | 306, 416, 711, 725, 1088, 1817 |
| BstDSI | CCRYGG | 1 cut(s) | 1388 |
| BstF5I | GGATG | 8 cut(s) | 40, 792, 811, 955, 1258, 1556, 1712, 1747 |
| BstFNI | CGCG | 2 cut(s) | 172, 386 |
| BstH2I | RGCGCY | 2 cut(s) | 154, 1120 |
| BstHHI | GCGC | 3 cut(s) | 153, 174, 1119 |
| BstKTI | GATC | 6 cut(s) | 85, 469, 885, 1147, 1177, 1555 |
| BstMAI | GTCTC | 1 cut(s) | 476 |
| BstMBI | GATC | 6 cut(s) | 82, 466, 882, 1144, 1174, 1552 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 16, 298, 307, 829, 1139, 1393 |
| BstNI | CCWGG | 4 cut(s) | 122, 1068, 1717, 1878 |
| BstNSI | RCATGY | 1 cut(s) | 1543 |
| BstSCI | CCNGG | 4 cut(s) | 120, 1066, 1715, 1876 |
| BstSFI | CTRYAG | 2 cut(s) | 154, 964 |
| BstUI | CGCG | 2 cut(s) | 172, 386 |
| BstV1I | GCAGC | 6 cut(s) | 140, 276, 941, 1084, 1201, 1481 |
| BstV2I | GAAGAC | 3 cut(s) | 781, 1221, 1377 |
| BstX2I | RGATCY | 4 cut(s) | 466, 1144, 1174, 1552 |
| BstYI | RGATCY | 4 cut(s) | 466, 1144, 1174, 1552 |
| BstZ17I | GTATAC | 1 cut(s) | 993 |
| BsuI | GTATCC | 1 cut(s) | 1496 |
| BsuRI | GGCC | 7 cut(s) | 590, 730, 754, 1184, 1365, 1396, 1461 |
| BtgI | CCRYGG | 1 cut(s) | 1388 |
| BtgZI | GCGATG | 1 cut(s) | 367 |
| BtsCI | GGATG | 8 cut(s) | 40, 792, 811, 955, 1258, 1556, 1712, 1747 |
| BtsIMutI | CAGTG | 6 cut(s) | 174, 457, 810, 886, 901, 1647 |
| BveI | ACCTGC | 1 cut(s) | 639 |
| Cac8I | GCNNGC | 4 cut(s) | 294, 834, 1039, 1140 |
| CciI | TCATGA | 2 cut(s) | 625, 856 |
| CfoI | GCGC | 3 cut(s) | 153, 174, 1119 |
| Cfr13I | GGNCC | 2 cut(s) | 184, 672 |
| CseI | GACGC | 1 cut(s) | 1310 |
| Csp6I | GTAC | 4 cut(s) | 1075, 1489, 1601, 1844 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1322, 1357 |
| CviQI | GTAC | 4 cut(s) | 1075, 1489, 1601, 1844 |
| DdeI | CTNAG | 6 cut(s) | 306, 416, 711, 725, 1088, 1817 |
| DpnI | GATC | 6 cut(s) | 84, 468, 884, 1146, 1176, 1554 |
| DpnII | GATC | 6 cut(s) | 82, 466, 882, 1144, 1174, 1552 |
| DraI | TTTAAA | 1 cut(s) | 1770 |
| DraIII | CACNNNGTG | 1 cut(s) | 907 |
| EaeI | YGGCCR | 3 cut(s) | 752, 1182, 1459 |
| Eam1104I | CTCTTC | 1 cut(s) | 353 |
| EarI | CTCTTC | 1 cut(s) | 353 |
| EciI | GGCGGA | 1 cut(s) | 1056 |
| Eco130I | CCWWGG | 4 cut(s) | 146, 431, 591, 1388 |
| Eco147I | AGGCCT | 2 cut(s) | 590, 730 |
| Eco47I | GGWCC | 2 cut(s) | 184, 672 |
| Eco57I | CTGAAG | 2 cut(s) | 216, 1220 |
| Eco88I | CYCGRG | 1 cut(s) | 1017 |
| EcoO109I | RGGNCCY | 1 cut(s) | 184 |
| EcoRII | CCWGG | 4 cut(s) | 120, 1066, 1715, 1876 |
| EcoT14I | CCWWGG | 4 cut(s) | 146, 431, 591, 1388 |
| EcoT22I | ATGCAT | 1 cut(s) | 1747 |
| ErhI | CCWWGG | 4 cut(s) | 146, 431, 591, 1388 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 306, 338, 593, 625, 773, 805 |
| FaqI | GGGAC | 2 cut(s) | 685, 1883 |
| FauNDI | CATATG | 2 cut(s) | 1495, 1849 |
| FblI | GTMKAC | 1 cut(s) | 992 |
| Fnu4HI | GCNGC | 8 cut(s) | 8, 127, 154, 290, 930, 1098, 1190, 1470 |
| FokI | GGATG | 8 cut(s) | 47, 779, 818, 942, 1265, 1543, 1699, 1754 |
| Fsp4HI | GCNGC | 8 cut(s) | 8, 127, 154, 290, 930, 1098, 1190, 1470 |
| GlaI | GCGC | 3 cut(s) | 152, 173, 1118 |
| GluI | GCNGC | 8 cut(s) | 8, 127, 154, 290, 930, 1098, 1190, 1470 |
| GsaI | CCCAGC | 1 cut(s) | 805 |
| GsuI | CTGGAG | 1 cut(s) | 471 |
| HaeII | RGCGCY | 2 cut(s) | 154, 1120 |
| HaeIII | GGCC | 7 cut(s) | 590, 730, 754, 1184, 1365, 1396, 1461 |
| HapII | CCGG | 1 cut(s) | 328 |
| HgaI | GACGC | 1 cut(s) | 1310 |
| HhaI | GCGC | 3 cut(s) | 153, 174, 1119 |
| Hin1I | GRCGYC | 1 cut(s) | 166 |
| Hin6I | GCGC | 3 cut(s) | 151, 172, 1117 |
| HinP1I | GCGC | 3 cut(s) | 151, 172, 1117 |
| HindIII | AAGCTT | 1 cut(s) | 1131 |
| HinfI | GANTC | 7 cut(s) | 362, 484, 860, 898, 1044, 1627, 1896 |
| HpaII | CCGG | 1 cut(s) | 328 |
| HphI | GGTGA | 5 cut(s) | 776, 1056, 1282, 1668, 1709 |
| Hpy166II | GTNNAC | 5 cut(s) | 649, 993, 1344, 1477, 1491 |
| Hpy188III | TCNNGA | 4 cut(s) | 95, 626, 857, 1019 |
| Hpy8I | GTNNAC | 5 cut(s) | 649, 993, 1344, 1477, 1491 |
| Hpy99I | CGWCG | 1 cut(s) | 1238 |
| HpyAV | CCTTC | 3 cut(s) | 371, 662, 1149 |
| HpyCH4III | ACNGT | 6 cut(s) | 503, 905, 1238, 1600, 1642, 1766 |
| HpyCH4IV | ACGT | 2 cut(s) | 166, 677 |
| HpyCH4V | TGCA | 7 cut(s) | 156, 292, 929, 1037, 1229, 1469, 1745 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 16, 298, 307, 829, 1139, 1393 |
| HpyF3I | CTNAG | 6 cut(s) | 306, 416, 711, 725, 1088, 1817 |
| HpySE526I | ACGT | 2 cut(s) | 166, 677 |
| Hsp92I | GRCGYC | 1 cut(s) | 166 |
| HspAI | GCGC | 3 cut(s) | 151, 172, 1117 |
| Kzo9I | GATC | 6 cut(s) | 82, 466, 882, 1144, 1174, 1552 |
| LguI | GCTCTTC | 1 cut(s) | 353 |
| LmnI | GCTCC | 6 cut(s) | 4, 306, 1084, 1327, 1708, 1832 |
| Lsp1109I | GCAGC | 6 cut(s) | 140, 276, 941, 1084, 1201, 1481 |
| LweI | GCATC | 4 cut(s) | 390, 796, 1243, 1732 |
| MaeII | ACGT | 2 cut(s) | 166, 677 |
| MaeIII | GTNAC | 4 cut(s) | 764, 907, 1450, 1882 |
| MalI | GATC | 6 cut(s) | 84, 468, 884, 1146, 1176, 1554 |
| MbiI | CCGCTC | 1 cut(s) | 346 |
| MboI | GATC | 6 cut(s) | 82, 466, 882, 1144, 1174, 1552 |
| MboII | GAAGA | 7 cut(s) | 209, 340, 542, 781, 1226, 1382, 1562 |
| MfeI | CAATTG | 2 cut(s) | 405, 1209 |
| MflI | RGATCY | 4 cut(s) | 466, 1144, 1174, 1552 |
| MhlI | GDGCHC | 1 cut(s) | 856 |
| MlsI | TGGCCA | 1 cut(s) | 1461 |
| MluNI | TGGCCA | 1 cut(s) | 1461 |
| MlyI | GAGTC | 5 cut(s) | 478, 854, 892, 1053, 1905 |
| MmeI | TCCRAC | 2 cut(s) | 1202, 1810 |
| Mox20I | TGGCCA | 1 cut(s) | 1461 |
| Mph1103I | ATGCAT | 1 cut(s) | 1747 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1865 |
| MscI | TGGCCA | 1 cut(s) | 1461 |
| MseI | TTAA | 7 cut(s) | 372, 561, 585, 975, 1032, 1260, 1769 |
| MslI | CAYNNNNRTG | 4 cut(s) | 212, 849, 1750, 1889 |
| Msp20I | TGGCCA | 1 cut(s) | 1461 |
| MspA1I | CMGCKG | 1 cut(s) | 1472 |
| MspI | CCGG | 1 cut(s) | 328 |
| MspR9I | CCNGG | 4 cut(s) | 122, 1068, 1717, 1878 |
| MunI | CAATTG | 2 cut(s) | 405, 1209 |
| Mva1269I | GAATGC | 2 cut(s) | 1745, 1807 |
| MvaI | CCWGG | 4 cut(s) | 122, 1068, 1717, 1878 |
| MvnI | CGCG | 2 cut(s) | 172, 386 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 16, 298, 307, 829, 1139, 1393 |
| NcoI | CCATGG | 1 cut(s) | 1388 |
| NdeI | CATATG | 2 cut(s) | 1495, 1849 |
| NdeII | GATC | 6 cut(s) | 82, 466, 882, 1144, 1174, 1552 |
| NlaIV | GGNNCC | 4 cut(s) | 186, 673, 1176, 1710 |
| NmeAIII | GCCGAG | 2 cut(s) | 520, 730 |
| NmuCI | GTSAC | 4 cut(s) | 764, 907, 1450, 1882 |
| NsiI | ATGCAT | 1 cut(s) | 1747 |
| NspI | RCATGY | 1 cut(s) | 1543 |
| OliI | CACNNNNGTG | 1 cut(s) | 212 |
| PaeR7I | CTCGAG | 1 cut(s) | 1017 |
| PagI | TCATGA | 2 cut(s) | 625, 856 |
| PceI | AGGCCT | 2 cut(s) | 590, 730 |
| PciSI | GCTCTTC | 1 cut(s) | 353 |
| PctI | GAATGC | 2 cut(s) | 1745, 1807 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1865 |
| PfeI | GAWTC | 2 cut(s) | 362, 1627 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 761 |
| PfoI | TCCNGGA | 1 cut(s) | 1715 |
| PkrI | GCNGC | 8 cut(s) | 9, 128, 155, 291, 931, 1099, 1191, 1471 |
| PleI | GAGTC | 5 cut(s) | 478, 854, 892, 1052, 1904 |
| PpsI | GAGTC | 5 cut(s) | 478, 854, 892, 1052, 1904 |
| PpuMI | RGGWCCY | 1 cut(s) | 184 |
| PshBI | ATTAAT | 1 cut(s) | 1032 |
| PsiI | TTATAA | 1 cut(s) | 1736 |
| Psp5II | RGGWCCY | 1 cut(s) | 184 |
| Psp6I | CCWGG | 4 cut(s) | 120, 1066, 1715, 1876 |
| PspFI | CCCAGC | 1 cut(s) | 801 |
| PspGI | CCWGG | 4 cut(s) | 120, 1066, 1715, 1876 |
| PspN4I | GGNNCC | 4 cut(s) | 186, 673, 1176, 1710 |
| PspPI | GGNCC | 2 cut(s) | 184, 672 |
| PspPPI | RGGWCCY | 1 cut(s) | 184 |
| PstI | CTGCAG | 1 cut(s) | 158 |
| PsuI | RGATCY | 4 cut(s) | 466, 1144, 1174, 1552 |
| PvuII | CAGCTG | 1 cut(s) | 1472 |
| RsaI | GTAC | 4 cut(s) | 1076, 1490, 1602, 1845 |
| RsaNI | GTAC | 4 cut(s) | 1075, 1489, 1601, 1844 |
| RseI | CAYNNNNRTG | 4 cut(s) | 212, 849, 1750, 1889 |
| SapI | GCTCTTC | 1 cut(s) | 353 |
| SaqAI | TTAA | 7 cut(s) | 372, 561, 585, 975, 1032, 1260, 1769 |
| SatI | GCNGC | 8 cut(s) | 8, 127, 154, 290, 930, 1098, 1190, 1470 |
| Sau3AI | GATC | 6 cut(s) | 82, 466, 882, 1144, 1174, 1552 |
| Sau96I | GGNCC | 2 cut(s) | 184, 672 |
| ScaI | AGTACT | 2 cut(s) | 1076, 1602 |
| SchI | GAGTC | 5 cut(s) | 478, 854, 892, 1053, 1905 |
| ScrFI | CCNGG | 4 cut(s) | 122, 1068, 1717, 1878 |
| SduI | GDGCHC | 1 cut(s) | 856 |
| SfaNI | GCATC | 4 cut(s) | 390, 796, 1243, 1732 |
| SfcI | CTRYAG | 2 cut(s) | 154, 964 |
| Sfr274I | CTCGAG | 1 cut(s) | 1017 |
| SinI | GGWCC | 2 cut(s) | 184, 672 |
| SlaI | CTCGAG | 1 cut(s) | 1017 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 212, 849, 1750, 1889 |
| SmlI | CTYRAG | 1 cut(s) | 1017 |
| SmoI | CTYRAG | 1 cut(s) | 1017 |
| SpeI | ACTAGT | 1 cut(s) | 209 |
| SseBI | AGGCCT | 2 cut(s) | 590, 730 |
| SsiI | CCGC | 4 cut(s) | 126, 170, 344, 1041 |
| StuI | AGGCCT | 2 cut(s) | 590, 730 |
| StyD4I | CCNGG | 4 cut(s) | 120, 1066, 1715, 1876 |
| StyI | CCWWGG | 4 cut(s) | 146, 431, 591, 1388 |
| TaaI | ACNGT | 6 cut(s) | 503, 905, 1238, 1600, 1642, 1766 |
| TaiI | ACGT | 2 cut(s) | 169, 680 |
| TaqI | TCGA | 5 cut(s) | 1018, 1531, 1572, 1667, 1749 |
| TatI | WGTACW | 3 cut(s) | 1074, 1488, 1600 |
| TauI | GCSGC | 1 cut(s) | 129 |
| TfiI | GAWTC | 2 cut(s) | 362, 1627 |
| Tru1I | TTAA | 7 cut(s) | 372, 561, 585, 975, 1032, 1260, 1769 |
| Tru9I | TTAA | 7 cut(s) | 372, 561, 585, 975, 1032, 1260, 1769 |
| TscAI | CASTG | 6 cut(s) | 181, 457, 817, 893, 908, 1647 |
| TseFI | GTSAC | 4 cut(s) | 764, 907, 1450, 1882 |
| TseI | GCWGC | 7 cut(s) | 7, 153, 289, 929, 1097, 1189, 1469 |
| Tsp45I | GTSAC | 4 cut(s) | 764, 907, 1450, 1882 |
| TspDTI | ATGAA | 6 cut(s) | 51, 246, 543, 781, 1383, 1415 |
| TspGWI | ACGGA | 1 cut(s) | 282 |
| TspRI | CASTG | 6 cut(s) | 181, 457, 817, 893, 908, 1647 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 761 |
| VpaK11BI | GGWCC | 2 cut(s) | 184, 672 |
| VspI | ATTAAT | 1 cut(s) | 1032 |
| XapI | RAATTY | 4 cut(s) | 12, 57, 509, 533 |
| XceI | RCATGY | 1 cut(s) | 1543 |
| XhoI | CTCGAG | 1 cut(s) | 1017 |
| XmaJI | CCTAGG | 1 cut(s) | 146 |
| XmiI | GTMKAC | 1 cut(s) | 992 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1865 |
| ZraI | GACGTC | 1 cut(s) | 167 |
| ZrmI | AGTACT | 2 cut(s) | 1076, 1602 |
| Zsp2I | ATGCAT | 1 cut(s) | 1747 |