MD04G1012900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000582
Physical Location & Seq
Reverse (-)
1445380 .. 1469290
23911 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1012900.v1.1.491

Sequence Viewer

Length: 8241 bp
ATGTACGGACGACCTTCTCGCCGCTCCTCCGGCGGCGGACGGGGTCAGCCACCGCAACAACCCCAAAACCCTAATTTCCCCCTCCAAAGCCCCATCAATTCACCATATGTCTATCCCTCCAACCCTGCCTTCATACCCCACAGTGCCTACCAAAACCCTTACCAAACCTTCCCTCCCACTAATTTCCCTATCCAAAACCCTAATTTCTCCCGCCAAAACGCTGGCTTCACTCCTCCTCCCCAGCAGGTTTCGAGCTCGACGGTCTTTCGGCCTCAGAACTCGAGAGATATGCTCGAGAGAATTGACCGCGCAGTGGGGAAGGCTCGCGACGAACTTGCCGCCGCAGGAGAGAACGTCTCCGCGTGGAAGGTCTCGCAGTCGGCCCTTCTGATGCTCAAGGTTGATAGCTGGGATTCTTTGGGGTTTCAGATGCAGCAAGTGCCGTCTCTGAACCGCCTCATGCTCACCGAAGCGAAGATAAATGCATTTATCCATTGTTTCGTTGGCGTTCAAACCATTACTTCATTGTATGATATGGAAGTAGCAATCTGCAAGAATGAGAGGATTGAGCAGTTTGAAGAGCTGGGATTGGGCCCTTTGTTGCGACACCCACTTGTTTTGCATTATTTCTCGGTGAAGTCTGATACAACTAAAGTGTTTAAGATAACTAGTTGGGAGATAATATCTCTACTTTCAGAGTTCATGGATACTTCTAACAATAAAAGGTTTAGCCCTGAAGAGATTTTAGATTTCATCGTTAAGAGGCGAGCAGCTGCAAGCAAGGATGATCTTGGAATACGAATTCACAGTGTGGGGAATTATATTTCGGCCATTCGGAAAGCCAGGAGATTAGAAAAGAAATCTGCGAAGGGATTTGAGCAAAAGTTAGATACAAAATGTAGGAAGCGTCCACTTTTCTCCGCACTGAAAAATCAGCTAGACGAACGTTTCAGCACCATTTCTCAGCGTGTTGAATCGTTTTCTTCCATGCATAAGGATTTTCGTGGCAAGCACATTAGATTTGATTCTTCGAGCTCAGATGATGAAGATAATGAGAATTATTCATCCGAAGATGATTTTGAAAATAATGACCATGCCACAGGTAGTCAGGTTAACCACTCAGCACAATCTGTTAAAAGTTCCGACCAAGTGAGCAGCTGTCCTTACCCGTCTGCAATAGAAGAGAGGACACGTCTTGGGTTGAGCCAGCTTTCTCCAGCTAGCGGCACCCAAAAGCACAATGAGAGTAGTGGATCTGTTAGAAAGAAAAGGAAATCTGAAAACATAAACAGTGCAATCTCCATTCCGCACAAATTGCGGAAGAGAGATAAGGTTCAAGATGCCCTTCCTGTGCAAAATGGTAGGGAAACCAATGAGGTCAGCAATTTGCATGAAGATGAACTTTCAGTTGACAATAACTATATGAAGATGTTTATTACAACCTGGAAGGAGGCATGTCGGGAGCACACTGTGACGGAGGTACTTGATAGAATGCTGCTGTATAATAATACTAAAGCTCAGAAGAGAAAGAAAATCAAGTCAATGTTTTCCCTAGATCCATTAATTGGATTACTAAATGTTGCTGTCTCGTCTATCAAATGTGGAATGTGGGACAGCATGTATGATACATTCCAAACTGTCAGTCAATATGAGTTAACTGGCAGTCTTACTGATAACTGTCCTGAATATGTGAGCATAGATGTTGAACCAGTTACCAAAGATGAACAAAAAATGAAGGATCCACGGGTCATCAGTGAACATATTGTTGAGCATATGCAGAGTGTTTCTGTTGAGGACGTAATCAGAAAAATTACCTTGTATTTTGAGAGTGATCAAGGAAGGCATAACGATAGTCAATCACTTCAGGAGAAGATATTTATCTTCTTGAGAAAGCTTTGTGATTGCGAGCTTTGGCTGGTCAAAGAATTTGGTGTCGAGGAATTTAAATCCCTCGGTCATGGGGAGTTTTTTATGTTCTTAGAGAAATATGCTTGTCTACTGCCACACGAGCTGTGCAAGTTCCTGACCGGTGATGTAACTGGGAAGTGTCCTTTGGAGGTTTGCTTGCTCCAACACCATTTGGTTGTTTTAGTATCTCAAGCTTTGAATAGTTTATGGGAGGATGAAAACATTACTAAAGAAAAGATATTACTGCTGCATAGGAAGCAGTTCCCATTGGTTAGTTTCACAACTATCGAAAATGGTTCAGTGGAAGATTTCTTAAGCATAGTGGGAAAGCATAAAAATGCTGCGATTTCCAAATGTGTTCAATTTTCAATGGCACTATGTGGAACTAGTAATGCTAGACAGTCATCAGCACAGATTGAGAATGTCCTATGGAACAGCACTTTAATCAATACTGACAGTGGCCAGAAAGCAAAGCCTTATGAATCTGTTACATCCAAGGATGCAATTGAAGTTTTACTTAGGGCACCAATGATGTCAGATTTAAACTTATGGTCACATTGGGACCTTTTGTTTGCTCCCTCTCTTGGTCCTCTTTTACCATGGCTGTTGAAGGAAGTCAACACGGATGAACTGTTGTGTTTGGTGACAAGAGATGGAAAACTGATTCGGTTAGATCATTCAGCAACTGTTGATTCATTCTTGGAAGCTGCACTTCAAGGATCCTCTTTTCAAACAGCAGTGAAAATGCTCTCTCTATTTTCACTAGTTGGGGGAGAAAAACATGTTCCTGTCTCCCTTCTGAAAATTCATATACAACATGCATTTGAAGTAATCCTGAAGAATTATTTAGATGGTTTGGAAGTACATGACAATAATAATTCTTTTAATTATGGAGAACCACTGTCTAGACAAGAAATTGTTGGTGAAGTTGCTGCTGGTAAATTGTGTAGTAAATTACACAGTGACTTAAGCAAGATGAATAGTGCAACGCCTGTTATATCAAGATTTGTCCTTGAATGTCTTGGGTATCTACCTACCGAGTTTCGTGATTTTGCTGCTGATATATTGCTTTCTGGGATACAATCTGTTGTCAAACATGCTCCTTCAGCCATTCTGGGTGAATGTATCAAATCAGAACAGCGTCTCATGCTTCATGAAGTTGGGTTGTCTCTTGGTATAGTTGAGTGGATTACTGATTACTATGAATTTTGTTCCAACGATGCTACTGACTTGTCCATATCTGCGGATTCACGTATGAATTCTGTTAGTTATGAAACAGGGTCAAGCTCAATGATTGTGCAAGATGTGTCAGGTAAGTTTTCTGCTTCTGAACAGAGCATGGGTGCATCTGGTAGGTCAGATGAGCAGACCCGTGGATGTACTGGTATCTGTATGAAGATTGATAGCGCAGAAGCTTCTGATGCTAGAAGTGGATATTGTTCCACACACAGTTCTGCGGAGCTTAATAAACATGAAGATGCAGCCCAGGTAATTGAGTCAATCCGGAGAGATGAATTCGGTCTTGATTCAAGCCTACAAAGTGTTGAAAGTATCATGCTAAAGAAGCAGCATGCTCGCTTAGGGAGAGCTCTTCATTGTCTTTCACAGGAATTGTATTCTCAGGATTCACACTTTCTTCTTGAGCTGGTTCAAAATGCTGATGATAATACCTACCCAACAAATGTAGAACCAACTCTAACATTCATTCTTCAAGAATCAAGTATTATTGTTTTAAACAATGAGCAAGGATTCTCTTCCCAGAATATTAGAGCACTTTGTGACGTTGGCAGTTCCACCAAGAAAGGATCTAATGCTGGATATATAGGCCAGAAGGGCATTGGTTTCAAATCAGTATTTCGGGTCACAGATGCTCCAGAAATTCATTCCAATGGGTTCCATATCAAATTTGATATAAGTGAGGGTCAGATCGGGTTTGTTTTGCCAACAGTTGTACCTCCGTGCAGTATTGACTTGTTTAGTAGGCTTACTTCTGGTGGCAGTGATCAATCGGACCGTGACTGCTGGAACACTTGCATTGTTCTTCCTTTCAAATCAAAGTTATCAGATGCAACTGTCATGAAGAACATTATAAATATGTTTTCAGATCTTCATCCATCTTTATTACTCTTTCTGCACCGCCTCCAGTGTATCAAGTTCAGAAACTTGCTCGATGATTCACTTACTGTCATGAGAAAAGAAATTGTGGGAGATGGTATCGTTAAGGTTTCACATGGGAAAGAAAAAATGACATGGTTTGTAGTATCTCAAAAATTGCAAGCTGAATTTGTTCGTAGTGATGTACAAAAAACAGAGATCTCGATAGCATTGACTTTGAAGGAATCCGATAGTGGCGATTATAGTCCAGATTTACGCCAACACCCGGTTTTTGCATTTCTTCCTCTAAGAACTTATGGCCTGAAATTTATTCTGCAGGGTGATTTTGCCATTACTTCATCTAGAGAGGAAGTGGATGGAGATAGTCCCTGGAACCAGTGGCTATTGTCAGAATTTCCAGGTTTGTTCATCAATGTTGAGAGATCTTTTTGTGCTCTTCCATGTTTTAGAGAGAATCCAGGCAGAGCTGTGGCAGCTTATATGTCCTTTGTTCCGCTTGTTGGGGAGGTGCATGGCTTCTTTTCTTCTCTTCCTCGATTGATTATTTCTAAATTACGTATGTCAAATTGCTTACTCCTAGAAGGTCGAAACAATGAATGGGTTCCTCCTTGCAAAGTCCTAAGAGGTTGGAATGAACGTGCTCATTCACTTCTTCCTGACAGTTTACTATGTGAACATCTTGGCCTTGGGTTCTTGGATAAAAATATTGTGCTGTCAGACCAACTTGCAAGTGCACTAGGTATTGCGGAGTATGGGCCCAAAGTTCTTCTTCAAGTTATGGTCTCTTTATGCCATAAACATAATGATATTAAATCAATGGGGTTTGGTTGGCTGGCCTTTTGGCTAAGTGAACTTTATGCAATGTTTTTCAGTTCATCTGTGGAAGCTTCATTTGACTCTAGAATTCAAATGGATGTCATAGAAAACCTTCGAAAAATTCCATTCATTCCTCTTTCAGATGGTACTTACGGTGCAATAGATGAAGGCCCAATTTGGTTACATTTTGATGCCTCATGCACTGAGCTTGAAGCTCAGCACGGACTTGAGTCCTTTCCACATTTATATGCAAAACTTCGGACCGTAAATCCTGCCCTCCTTTCTACACTGTCTGCAGACATGTCATCCATGGATGTGACGACAGTTGAAAAACTCACGTGTATGCTTTTCCGAATTGGTGTCCAGAAGTTGTCTACACACGAAATTGTCAAAGTGCACATCCTGCCAGCTATGTCTGAAGATAGAATTATGGATAGGGATAAGAATTTGATGATTGAATATCTCTGCTTTGTTATGCTCCACCTACAGTCTAGCTGCTCCGATTGTCATGTTGAAAGGGAATATATAATCTCTGAAATACTGGATAAGGCTTACATATCAACAAATCATGGCTTTAAACGACCTTCTGAGGTATCAATTCATTTCAGTCAAGAATTTGGTAATCCCGTGGACATAAAGAAGTTGATTAATATGGTGGATATGAAGTGGCATGAGGTTGACATCTCCTATTTGAGACATCCTGTTTCAAAGTCACTCCCATGTGAACTGGAGAAGTGGAGGCAATTTTTCCAGCAAATTGGGATAATGGATTTCGTCAAAGTAGTTCAAGTTGAGAAGGGAATAGCTGATATTTCAGATGGAGTAGTTAAGAATTTGATATGGGATAAGGACTTAATTTCCCTTAGGCCAAATATCACAGATTGGGAGTCCCCTGAATTGGTCGACTTATTGTCAGTATTGGCCAGAGATGGCAACAAAAGAGGTTGTGAATATCTTTTAGAGGTTCTTGATACTTTGTGGGATGATTGTTATAGTGAAAAAACTACTGGTTATTGTGCTTCTAACTCTGAAGCCGACAGAAGGCCCTTCAAATCTTCATTTATAAGTAGCATCTGTGATGTGCAGTGGGTAGCATCAGCGATGGATGATGTGCTTCACTATCCTAAACATTTATATCATGACTGTGATGCAGTGCGTTCGACTCTGGGGGCATCTGCTCCTTTTGCGATTCCAAAGGTGAGAAGTGAGAATTTCGTACGTGATATTGGCTTTAAGACAATAGTTTCTCTTGACGATGCTCTGGAGGTCTTGAAGTTATGGAGGCGTGAAAATGAATTCCAGGCCAGCCTAGGGCAAATGTTGAAATTTTACACATTAATCTGGAATGAAATGGCTGCTTCAACGGAGAAAATTGCAGAGGTTTTCCATTCAGGACCTTCTATATTTATTCCTTATGCATCTAGCTTCAGGCACGAGGATGTGGTATGTGGTACATTGTTGTCCCCTGCAGAAGTATACTGGCATGATTCCACGTCGTTTGTAGACCAAATCAGAGAAATTCATCGTCAGTGCAGCTCAGCAGGTGTTGCTCATGGCCCATTGATTAAGATGCTTTGCAATTTCTACCCAGGCCTTCATGACTTCTTTGTTAACGTCTGTGGAGTACATGAAACCCCTCCTCTCCGAAGCTACATTCAGATTTTGCTTCATTTATCAAATGTTGCTTTGCCTTCACAAGCAGCTAGTGCAGTTTTTCAAGTTTTCCTGAAGTGGACTGATGGATTAAAATCAGGATTGAGTGCTGAGGATGTTCTTTATTTGAAAGATTTGCTTACAAAGATTGAATGTACGGTGCTTCCTACTTTACAAGATAAGTGGGTTTCTCTACATCCGTCATTTGGTCTTGTGTGCTGGTGTGATGATAAGAAATTAAGCAAGCAGTTTAAGCACTTGGATGGCATTGATTTTCTGTACTTGGGTGAACTGAGTAAAGATGATGAAGAGATGCTATGTACGAAAGTGTCCATCCTCATGCAGACTTTAGGGATTCCTGCTCTTTCAGAGGTTGTAACTCGCGAGGCAATATATTCTGGTATCGAAGACTCTAGCTTTAAAGCTGCATTGCTGGATTGGGCTCTTCCATATGCTCAGCGGTACTTAAATGGTGTACACCCAGATAAATATTCTCAACTCAAGCAGTCTGAATTTCTGAACTGTTTGCAAGTAGTAGTTGTTGAAAAGCTGTTTTACCGGAATGTTATAAAGGGTACTGGAAGTGAATCCAAGAAGCAAGTTGAATGTAGTTGCCTTCTGACGGGTAGCATTTTGTATACAACCCAAGAGTCAGATTCTCACGCATTATTTCTGGAGCTTTCTCGTTTATTTTTTAATGGAAGTCCTGAACTGCACTTTGCAAATTTCCTTCATATGATCACAACCATGGCAGAGTCAGGTTCAAATGAGGAGCAGACAGAGTTTTTCATCTTAAATAGCCAAAAGGTGCCAAAGCTTCCTGATGGTGAATCTGTTTGGTCCTTGTCTTCTGTGCATTCCTTAACGAGGAGTGATAAACCACTGCAGACAAGTTCTATTTCAACCGTAGTAAATGAACAAAGCAGTTTGAAATCCAAAAGCAAAGCAGCAACCTGGCCTCCTGTTGATTGGAAAACTGCACCAGGGTTTGGTTATGCTCGTGCAAATGGTTTTAAGACGCAGGCACCAGGTTCACAGCCTAACACGGTTGTACAAAGTAAATTGGGTGATGATTCTGAAGGTATCAGCAGACAAACAGATAATTTGACACCTGTTTCGGTTGACACTAATTGGACAATTGAACATTGTTCAGCAACATCTGCAGCTTTAGTTTTGCCAGACTCCAATCGTCTGCAAGAACATTGTAGTGAGGCTGACAATGAGGCTGACTTGTCAATGCATATCCAGTTTGATCCTAGTAGTTTTGGTCCTGTGTCTGATGCTCCTGACTTTGGTTCATCTAATTTTAGCAAGAGAGATCAGCTTAGATTTGGTACACCCAATTCGACACAAGCAATTTTAACTGGGCGGCTAGGTGAGCTTGTTGCTTTTAAATATTTTGTTGACAAAGCTGGCAAGTCAGTTGTTAAGTGGGTTAATGAACATAACGAAACTGGGCTACCATATGACATAGTTCTGGGAGGGAAGGAAAATAGTAGAGAGTACATTGAAGTTAAAGCAACCAAGTCTACAAGAAAAGACTGGTTTGAAATATCAATGAGAGAGTTGCAATTTGCGGTTGAGAAAGGTGAAGCTTTCAGTATTGCACATGTCGTTTTATTGGATAATAATGTTGCAAGGGTCTCAGTATTCAACAATCTTGCAAAATTGTGCCAGCTTCACAAACTACGTTTGGCTGTCTTGATGCCTGAGCAGCAGAAGGAATTTTCCATTGTTTCCCAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

2747

Amino Acids

308.39

Weight (kDa)

5.75

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 1175 - 1270 4.6e-09 Sacsin domain
NOV_C PF13020 2623 - 2708 1.2e-18 Protein NO VEIN, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 3998, 5903, 7061
AarI CACCTGC 1 cut(s) 6371
Acc36I ACCTGC 2 cut(s) 235, 6371
AccB1I GGYRCC 4 cut(s) 1226, 2431, 7300, 7516
AccB7I CCANNNNNTGG 2 cut(s) 1565, 7481
AccBSI CCGCTC 1 cut(s) 24
AccI GTMKAC 7 cut(s) 1996, 5213, 5742, 6315, 6342, 7130, 8021
AccII CGCG 4 cut(s) 309, 327, 362, 6876
AccIII TCCGGA 1 cut(s) 3411
AclI AACGTT 1 cut(s) 946
AclWI GGATC 8 cut(s) 1261, 1550, 1733, 1746, 2622, 2635, 3721, 7739
AcoI YGGCCR 3 cut(s) 828, 2368, 5760
AcuI CTGAAG 9 cut(s) 756, 1847, 2765, 2997, 5277, 5889, 6250, 6587, 7590
AcvI CACGTG 1 cut(s) 5178
AdeI CACNNNGTG 4 cut(s) 811, 1471, 2288, 3686
AflII CTTAAG 2 cut(s) 2221, 2875
AflIII ACRYGT 5 cut(s) 1190, 2689, 5139, 5177, 8101
AgeI ACCGGT 1 cut(s) 2027
AhdI GACNNNNNGTC 1 cut(s) 5749
AhlI ACTAGT 3 cut(s) 668, 2294, 2671
AjiI CACGTC 2 cut(s) 1193, 6333
AjuI GAANNNNNNNTTGG 4 cut(s) 113, 145, 2036, 2068
Alw21I GWGCWC 9 cut(s) 257, 1037, 1467, 3499, 3682, 4459, 4666, 4759, 5238
Alw44I GTGCAC 2 cut(s) 4755, 5234
AlwI GGATC 8 cut(s) 1261, 1550, 1733, 1746, 2622, 2635, 3721, 7739
AlwNI CAGNNNCTG 1 cut(s) 2594
Ama87I CYCGRG 2 cut(s) 280, 293
Aor13HI TCCGGA 1 cut(s) 3411
ApaI GGGCCC 2 cut(s) 596, 4782
ApaLI GTGCAC 2 cut(s) 4755, 5234
ArsI GACNNNNNNTTYG 6 cut(s) 1627, 1659, 2462, 2494, 7591, 7623
AseI ATTAAT 3 cut(s) 1562, 5487, 6176
AsiGI ACCGGT 1 cut(s) 2027
Asp700I GAANNNNTTC 4 cut(s) 2168, 4194, 4623, 4950
AspA2I CCTAGG 1 cut(s) 6148
AspLEI GCGC 2 cut(s) 311, 3317
AsuC2I CCSGG 1 cut(s) 4289
AsuII TTCGAA 1 cut(s) 4954
AsuNHI GCTAGC 1 cut(s) 1220
AvaI CYCGRG 2 cut(s) 280, 293
AvaII GGWCC 7 cut(s) 2470, 2495, 3919, 5100, 6233, 7332, 7760
AvrII CCTAGG 1 cut(s) 6148
AxyI CCTNAGG 1 cut(s) 5702
BaeGI GKGCMC 5 cut(s) 596, 2434, 4759, 4782, 5238
BaeI ACNNNNGTAYC 2 cut(s) 3843, 3876
BalI TGGCCA 2 cut(s) 2370, 5762
BamHI GGATCC 2 cut(s) 1738, 2627
BanI GGYRCC 4 cut(s) 1226, 2431, 7300, 7516
BanII GRGCYC 6 cut(s) 257, 596, 1037, 3499, 4782, 6937
BarI GAAGNNNNNNTAC 2 cut(s) 3880, 3912
BauI CACGAG 3 cut(s) 2006, 6272, 7491
BbrPI CACGTG 1 cut(s) 5178
BbsI GAAGAC 2 cut(s) 6906, 7332
Bbv12I GWGCWC 9 cut(s) 257, 1037, 1467, 3499, 3682, 4459, 4666, 4759, 5238
BbvCI CCTCAGC 1 cut(s) 6603
BceAI ACGGC 1 cut(s) 427
BcgI CGANNNNNNTGC 8 cut(s) 271, 305, 317, 351, 3464, 3498, 5979, 6013
BciVI GTATCC 2 cut(s) 700, 2979
BclI TGATCA 3 cut(s) 1831, 3910, 7230
BcnI CCSGG 1 cut(s) 4289
BcuI ACTAGT 3 cut(s) 668, 2294, 2671
BfmI CTRYAG 6 cut(s) 4337, 5133, 5324, 6306, 7376, 7653
BfrI CTTAAG 2 cut(s) 2221, 2875
BfuAI ACCTGC 2 cut(s) 235, 6371
BfuI GTATCC 2 cut(s) 700, 2979
BglI GCCNNNNNGGC 1 cut(s) 3741
BglII AGATCT 3 cut(s) 4012, 4221, 4445
BlnI CCTAGG 1 cut(s) 6148
BlpI GCTNAGC 3 cut(s) 5055, 6376, 6948
Bme18I GGWCC 7 cut(s) 2470, 2495, 3919, 5100, 6233, 7332, 7760
BmeRI GACNNNNNGTC 1 cut(s) 5749
BmeT110I CYCGRG 2 cut(s) 280, 293
BmgBI CACGTC 2 cut(s) 1193, 6333
BmrI ACTGGG 4 cut(s) 2049, 7866, 7956, 8227
BmtI GCTAGC 1 cut(s) 1224
BmuI ACTGGG 4 cut(s) 2049, 7866, 7956, 8227
BoxI GACNNNNGTC 1 cut(s) 5068
BpiI GAAGAC 2 cut(s) 6906, 7332
BplI GAGNNNNNCTC 4 cut(s) 276, 308, 341, 373
BpmI CTGGAG 6 cut(s) 1200, 3765, 4034, 5588, 6122, 7187
Bpu10I CCTNAGC 3 cut(s) 3487, 6603, 8202
Bpu1102I GCTNAGC 3 cut(s) 5055, 6376, 6948
Bpu14I TTCGAA 1 cut(s) 4954
BpuEI CTTGAG 6 cut(s) 380, 1906, 2082, 3569, 5087, 6977
BpuMI CCSGG 1 cut(s) 4289
BsaAI YACGTR 4 cut(s) 3161, 4580, 5178, 6059
BsaBI GATNNNNATC 3 cut(s) 1877, 4017, 6586
BsaI GGTCTC 3 cut(s) 376, 4810, 8140
BsaWI WCCGGW 3 cut(s) 2027, 3411, 7050
Bse118I RCCGGY 1 cut(s) 2027
Bse21I CCTNAGG 1 cut(s) 5702
Bse3DI GCAATG 2 cut(s) 4890, 6920
Bse8I GATNNNNATC 3 cut(s) 1877, 4017, 6586
BseAI TCCGGA 1 cut(s) 3411
BseJI GATNNNNATC 3 cut(s) 1877, 4017, 6586
BseMI GCAATG 2 cut(s) 4890, 6920
BseRI GAGGAG 6 cut(s) 16, 222, 225, 6468, 7277, 7375
BseSI GKGCMC 5 cut(s) 596, 2434, 4759, 4782, 5238
BseYI CCCAGC 3 cut(s) 240, 408, 583
BsgI GTGCAG 8 cut(s) 2601, 3889, 4025, 5942, 6391, 6567, 7190, 7455
Bsh1236I CGCG 4 cut(s) 309, 327, 362, 6876
BshNI GGYRCC 4 cut(s) 1226, 2431, 7300, 7516
BshTI ACCGGT 1 cut(s) 2027
BsiHKAI GWGCWC 9 cut(s) 257, 1037, 1467, 3499, 3682, 4459, 4666, 4759, 5238
BsiHKCI CYCGRG 2 cut(s) 280, 293
BsiSI CCGG 5 cut(s) 30, 2028, 3412, 4289, 7051
BsiWI CGTACG 1 cut(s) 6055
BslFI GGGAC 5 cut(s) 1625, 2483, 4374, 5713, 6286
BsmBI CGTCTC 3 cut(s) 361, 450, 3056
BsmFI GGGAC 5 cut(s) 1625, 2483, 4374, 5713, 6286
BsmI GAATGC 2 cut(s) 1497, 7348
Bso31I GGTCTC 3 cut(s) 376, 4810, 8140
BsoBI CYCGRG 2 cut(s) 280, 293
Bsp119I TTCGAA 1 cut(s) 4954
Bsp120I GGGCCC 2 cut(s) 592, 4778
Bsp13I TCCGGA 1 cut(s) 3411
Bsp1407I TGTACA 3 cut(s) 4207, 6967, 7543
Bsp1720I GCTNAGC 3 cut(s) 5055, 6376, 6948
Bsp19I CCATGG 3 cut(s) 2507, 5148, 7239
Bsp68I TCGCGA 2 cut(s) 327, 6876
BspEI TCCGGA 1 cut(s) 3411
BspFNI CGCG 4 cut(s) 309, 327, 362, 6876
BspHI TCATGA 5 cut(s) 3061, 3984, 4095, 5977, 6436
BspMAI CTGCAG 5 cut(s) 4341, 5137, 6310, 7380, 7657
BspMI ACCTGC 2 cut(s) 235, 6371
BspOI GCTAGC 1 cut(s) 1224
BspPI GGATC 8 cut(s) 1261, 1550, 1733, 1746, 2622, 2635, 3721, 7739
BspQI GCTCTTC 4 cut(s) 573, 3504, 4464, 6942
BspT104I TTCGAA 1 cut(s) 4954
BspT107I GGYRCC 4 cut(s) 1226, 2431, 7300, 7516
BspTI CTTAAG 2 cut(s) 2221, 2875
BspTNI GGTCTC 3 cut(s) 376, 4810, 8140
BsrBI CCGCTC 1 cut(s) 24
BsrDI GCAATG 2 cut(s) 4890, 6920
BsrFI RCCGGY 1 cut(s) 2027
BsrGI TGTACA 3 cut(s) 4207, 6967, 7543
BssAI RCCGGY 1 cut(s) 2027
BssNAI GTATAC 2 cut(s) 6316, 7131
BssSI CACGAG 3 cut(s) 2006, 6272, 7491
BssT1I CCWWGG 6 cut(s) 2403, 2507, 4708, 5148, 6148, 7239
Bst1107I GTATAC 2 cut(s) 6316, 7131
Bst2BI CACGAG 3 cut(s) 2006, 6272, 7491
BstAFI CTTAAG 2 cut(s) 2221, 2875
BstAPI GCANNNNNTGC 6 cut(s) 439, 1315, 5242, 6386, 6545, 7652
BstAUI TGTACA 3 cut(s) 4207, 6967, 7543
BstBAI YACGTR 4 cut(s) 3161, 4580, 5178, 6059
BstBI TTCGAA 1 cut(s) 4954
BstDSI CCRYGG 6 cut(s) 1742, 2507, 3280, 5148, 5466, 7239
BstENI CCTNNNNNAGG 1 cut(s) 7357
BstFNI CGCG 4 cut(s) 309, 327, 362, 6876
BstHHI GCGC 2 cut(s) 311, 3317
BstNSI RCATGY 8 cut(s) 1458, 1621, 2693, 2729, 3008, 3482, 5143, 8105
BstPAI GACNNNNGTC 1 cut(s) 5068
BstSFI CTRYAG 6 cut(s) 4337, 5133, 5324, 6306, 7376, 7653
BstSLI GKGCMC 5 cut(s) 596, 2434, 4759, 4782, 5238
BstSNI TACGTA 1 cut(s) 4580
BstUI CGCG 4 cut(s) 309, 327, 362, 6876
BstV2I GAAGAC 2 cut(s) 6906, 7332
BstX2I RGATCY 8 cut(s) 1253, 1555, 1738, 2627, 3713, 4012, 4221, 4445
BstXI CCANNNNNNTGG 2 cut(s) 221, 5597
BstYI RGATCY 8 cut(s) 1253, 1555, 1738, 2627, 3713, 4012, 4221, 4445
BstZ17I GTATAC 2 cut(s) 6316, 7131
Bsu36I CCTNAGG 1 cut(s) 5702
BsuI GTATCC 2 cut(s) 700, 2979
BtgI CCRYGG 6 cut(s) 1742, 2507, 3280, 5148, 5466, 7239
BtgZI GCGATG 1 cut(s) 5954
BtrI CACGTC 2 cut(s) 1193, 6333
BtsI GCAGTG 6 cut(s) 318, 2652, 3913, 5930, 5997, 7373
BtuMI TCGCGA 2 cut(s) 327, 6876
BveI ACCTGC 2 cut(s) 235, 6371
CaiI CAGNNNCTG 1 cut(s) 2594
CciI TCATGA 5 cut(s) 3061, 3984, 4095, 5977, 6436
CfoI GCGC 2 cut(s) 311, 3317
Cfr10I RCCGGY 1 cut(s) 2027
CpoI CGGWCCG 2 cut(s) 3919, 5100
CseI GACGC 3 cut(s) 896, 3038, 7519
CsiI ACCWGGT 1 cut(s) 7519
CspAI ACCGGT 1 cut(s) 2027
CspCI CAANNNNNGTGG 6 cut(s) 600, 635, 6319, 6354, 6656, 6691
CspI CGGWCCG 2 cut(s) 3919, 5100
DraI TTTAAA 6 cut(s) 1945, 2451, 3642, 5416, 6913, 7885
DraIII CACNNNGTG 4 cut(s) 811, 1471, 2288, 3686
DriI GACNNNNNGTC 1 cut(s) 5749
EaeI YGGCCR 3 cut(s) 828, 2368, 5760
Eam1105I GACNNNNNGTC 1 cut(s) 5749
EciI GGCGGA 1 cut(s) 51
Ecl136II GAGCTC 3 cut(s) 255, 1035, 3497
Eco105I TACGTA 1 cut(s) 4580
Eco130I CCWWGG 6 cut(s) 2403, 2507, 4708, 5148, 6148, 7239
Eco147I AGGCCT 1 cut(s) 6432
Eco24I GRGCYC 6 cut(s) 257, 596, 1037, 3499, 4782, 6937
Eco31I GGTCTC 3 cut(s) 376, 4810, 8140
Eco47I GGWCC 7 cut(s) 2470, 2495, 3919, 5100, 6233, 7332, 7760
Eco53kI GAGCTC 3 cut(s) 255, 1035, 3497
Eco57I CTGAAG 9 cut(s) 756, 1847, 2765, 2997, 5277, 5889, 6250, 6587, 7590
Eco72I CACGTG 1 cut(s) 5178
Eco81I CCTNAGG 1 cut(s) 5702
Eco88I CYCGRG 2 cut(s) 280, 293
EcoICRI GAGCTC 3 cut(s) 255, 1035, 3497
EcoNI CCTNNNNNAGG 1 cut(s) 7357
EcoO109I RGGNCCY 4 cut(s) 593, 2470, 5883, 6233
EcoRI GAATTC 5 cut(s) 801, 3166, 3422, 4926, 6134
EcoT14I CCWWGG 6 cut(s) 2403, 2507, 4708, 5148, 6148, 7239
EcoT22I ATGCAT 5 cut(s) 487, 993, 2731, 6259, 7734
EcoT38I GRGCYC 6 cut(s) 257, 596, 1037, 3499, 4782, 6937
ErhI CCWWGG 6 cut(s) 2403, 2507, 4708, 5148, 6148, 7239
Esp3I CGTCTC 3 cut(s) 361, 450, 3056
FaqI GGGAC 5 cut(s) 1625, 2483, 4374, 5713, 6286
FauI CCCGC 1 cut(s) 218
FauNDI CATATG 5 cut(s) 106, 1773, 6943, 7227, 7957
FbaI TGATCA 3 cut(s) 1831, 3910, 7230
FblI GTMKAC 7 cut(s) 1996, 5213, 5742, 6315, 6342, 7130, 8021
FriOI GRGCYC 6 cut(s) 257, 596, 1037, 3499, 4782, 6937
GlaI GCGC 2 cut(s) 310, 3316
GsaI CCCAGC 3 cut(s) 244, 412, 587
GsuI CTGGAG 6 cut(s) 1200, 3765, 4034, 5588, 6122, 7187
HapII CCGG 5 cut(s) 30, 2028, 3412, 4289, 7051
HgaI GACGC 3 cut(s) 896, 3038, 7519
HhaI GCGC 2 cut(s) 311, 3317
Hin6I GCGC 2 cut(s) 309, 3315
HinP1I GCGC 2 cut(s) 309, 3315
HincII GTYRAC 9 cut(s) 1114, 1411, 1656, 2527, 5518, 5743, 6451, 7615, 7897
HindII GTYRAC 9 cut(s) 1114, 1411, 1656, 2527, 5518, 5743, 6451, 7615, 7897
HindIII AAGCTT 6 cut(s) 1892, 2100, 3321, 4908, 7307, 8085
HpaI GTTAAC 3 cut(s) 1114, 1656, 6451
HpaII CCGG 5 cut(s) 30, 2028, 3412, 4289, 7051
Hpy99I CGWCG 3 cut(s) 262, 332, 6337
HspAI GCGC 2 cut(s) 309, 3315
Kpn2I TCCGGA 1 cut(s) 3411
Ksp22I TGATCA 3 cut(s) 1831, 3910, 7230
KspAI GTTAAC 3 cut(s) 1114, 1656, 6451
LguI GCTCTTC 4 cut(s) 573, 3504, 4464, 6942
MabI ACCWGGT 1 cut(s) 7519
MbiI CCGCTC 1 cut(s) 24
MfeI CAATTG 2 cut(s) 2412, 7629
MflI RGATCY 8 cut(s) 1253, 1555, 1738, 2627, 3713, 4012, 4221, 4445
MlsI TGGCCA 2 cut(s) 2370, 5762
MluNI TGGCCA 2 cut(s) 2370, 5762
MlyI GAGTC 9 cut(s) 3413, 4913, 5078, 5735, 5995, 6896, 7151, 7256, 7667
MmeI TCCRAC 5 cut(s) 144, 1167, 2095, 3147, 4631
Mox20I TGGCCA 2 cut(s) 2370, 5762
Mph1103I ATGCAT 5 cut(s) 487, 993, 2731, 6259, 7734
MroI TCCGGA 1 cut(s) 3411
MroXI GAANNNNTTC 4 cut(s) 2168, 4194, 4623, 4950
MscI TGGCCA 2 cut(s) 2370, 5762
Msp20I TGGCCA 2 cut(s) 2370, 5762
MspA1I CMGCKG 3 cut(s) 773, 1158, 6952
MspCI CTTAAG 2 cut(s) 2221, 2875
MspI CCGG 5 cut(s) 30, 2028, 3412, 4289, 7051
MunI CAATTG 2 cut(s) 2412, 7629
Mva1269I GAATGC 2 cut(s) 1497, 7348
MvnI CGCG 4 cut(s) 309, 327, 362, 6876
NciI CCSGG 1 cut(s) 4289
NcoI CCATGG 3 cut(s) 2507, 5148, 7239
NdeI CATATG 5 cut(s) 106, 1773, 6943, 7227, 7957
NheI GCTAGC 1 cut(s) 1220
NmuCI GTSAC 9 cut(s) 1471, 2460, 2551, 2870, 3686, 3769, 3923, 5155, 5550
NruI TCGCGA 2 cut(s) 327, 6876
NsiI ATGCAT 5 cut(s) 487, 993, 2731, 6259, 7734
NspI RCATGY 8 cut(s) 1458, 1621, 2693, 2729, 3008, 3482, 5143, 8105
NspV TTCGAA 1 cut(s) 4954
PaeI GCATGC 1 cut(s) 3482
PaeR7I CTCGAG 2 cut(s) 280, 293
PagI TCATGA 5 cut(s) 3061, 3984, 4095, 5977, 6436
PaqCI CACCTGC 1 cut(s) 6371
PceI AGGCCT 1 cut(s) 6432
PciI ACATGT 3 cut(s) 2689, 5139, 8101
PciSI GCTCTTC 4 cut(s) 573, 3504, 4464, 6942
PctI GAATGC 2 cut(s) 1497, 7348
PdmI GAANNNNTTC 4 cut(s) 2168, 4194, 4623, 4950
Pfl23II CGTACG 1 cut(s) 6055
PflFI GACNNNGTC 1 cut(s) 42
PflMI CCANNNNNTGG 2 cut(s) 1565, 7481
PinAI ACCGGT 1 cut(s) 2027
PleI GAGTC 9 cut(s) 3412, 4913, 5077, 5734, 5995, 6896, 7150, 7255, 7667
PmaCI CACGTG 1 cut(s) 5178
PmlI CACGTG 1 cut(s) 5178
PpsI GAGTC 9 cut(s) 3412, 4913, 5077, 5734, 5995, 6896, 7150, 7255, 7667
Ppu21I YACGTR 4 cut(s) 3161, 4580, 5178, 6059
PpuMI RGGWCCY 2 cut(s) 2470, 6233
PscI ACATGT 3 cut(s) 2689, 5139, 8101
PshAI GACNNNNGTC 1 cut(s) 5068
PshBI ATTAAT 3 cut(s) 1562, 5487, 6176
PsiI TTATAA 3 cut(s) 3998, 5903, 7061
Psp124BI GAGCTC 3 cut(s) 257, 1037, 3499
Psp1406I AACGTT 1 cut(s) 946
Psp5II RGGWCCY 2 cut(s) 2470, 6233
PspCI CACGTG 1 cut(s) 5178
PspFI CCCAGC 3 cut(s) 240, 408, 583
PspLI CGTACG 1 cut(s) 6055
PspOMI GGGCCC 2 cut(s) 592, 4778
PspPPI RGGWCCY 2 cut(s) 2470, 6233
PstI CTGCAG 5 cut(s) 4341, 5137, 6310, 7380, 7657
PstNI CAGNNNCTG 1 cut(s) 2594
PsuI RGATCY 8 cut(s) 1253, 1555, 1738, 2627, 3713, 4012, 4221, 4445
PsyI GACNNNGTC 1 cut(s) 42
PvuII CAGCTG 2 cut(s) 773, 1158
RruI TCGCGA 2 cut(s) 327, 6876
Rsr2I CGGWCCG 2 cut(s) 3919, 5100
RsrII CGGWCCG 2 cut(s) 3919, 5100
SacI GAGCTC 3 cut(s) 257, 1037, 3499
SalI GTCGAC 1 cut(s) 5741
SapI GCTCTTC 4 cut(s) 573, 3504, 4464, 6942
SchI GAGTC 9 cut(s) 3413, 4913, 5078, 5735, 5995, 6896, 7151, 7256, 7667
SexAI ACCWGGT 1 cut(s) 7519
SfcI CTRYAG 6 cut(s) 4337, 5133, 5324, 6306, 7376, 7653
Sfr274I CTCGAG 2 cut(s) 280, 293
SfuI TTCGAA 1 cut(s) 4954
SinI GGWCC 7 cut(s) 2470, 2495, 3919, 5100, 6233, 7332, 7760
SlaI CTCGAG 2 cut(s) 280, 293
SmiI ATTTAAAT 1 cut(s) 1945
SnaBI TACGTA 1 cut(s) 4580
SpeI ACTAGT 3 cut(s) 668, 2294, 2671
SphI GCATGC 1 cut(s) 3482
SseBI AGGCCT 1 cut(s) 6432
SspI AATATT 4 cut(s) 3673, 4729, 6983, 7889
SstI GAGCTC 3 cut(s) 257, 1037, 3499
StuI AGGCCT 1 cut(s) 6432
StyI CCWWGG 6 cut(s) 2403, 2507, 4708, 5148, 6148, 7239
SwaI ATTTAAAT 1 cut(s) 1945
TaqII GACCGA 2 cut(s) 1943, 3416
TatI WGTACW 8 cut(s) 2770, 3287, 4207, 6463, 6771, 6967, 7543, 7995
TauI GCSGC 6 cut(s) 24, 36, 341, 344, 1227, 7864
TseFI GTSAC 9 cut(s) 1471, 2460, 2551, 2870, 3686, 3769, 3923, 5155, 5550
Tsp45I GTSAC 9 cut(s) 1471, 2460, 2551, 2870, 3686, 3769, 3923, 5155, 5550
TspGWI ACGGA 7 cut(s) 21, 1490, 2546, 3855, 5076, 6218, 6681
Tth111I GACNNNGTC 1 cut(s) 42
Van91I CCANNNNNTGG 2 cut(s) 1565, 7481
Vha464I CTTAAG 2 cut(s) 2221, 2875
VneI GTGCAC 2 cut(s) 4755, 5234
VpaK11BI GGWCC 7 cut(s) 2470, 2495, 3919, 5100, 6233, 7332, 7760
VspI ATTAAT 3 cut(s) 1562, 5487, 6176
XagI CCTNNNNNAGG 1 cut(s) 7357
XbaI TCTAGA 3 cut(s) 2813, 4364, 4922
XceI RCATGY 8 cut(s) 1458, 1621, 2693, 2729, 3008, 3482, 5143, 8105
XcmI CCANNNNNNNNNTGG 2 cut(s) 500, 3743
XhoI CTCGAG 2 cut(s) 280, 293
XmaJI CCTAGG 1 cut(s) 6148
XmiI GTMKAC 7 cut(s) 1996, 5213, 5742, 6315, 6342, 7130, 8021
XmnI GAANNNNTTC 4 cut(s) 2168, 4194, 4623, 4950
Zsp2I ATGCAT 5 cut(s) 487, 993, 2731, 6259, 7734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.