MD04G1013100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000582
Physical Location & Seq
Reverse (-)
1496678 .. 1500010
3333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1013100.v1.1.491

Sequence Viewer

Length: 1803 bp
ATGGTTTCTCTTGACGATGCTCTGGAGGTCCTGAAGTTATGGAGGCGTGAAAACGAATTCCAGGCCAGCCTAGGGCAAATGTTGAAATTTTACACATTAATCTGGAATGAAATGGCTGCTTCAACGGAGAAAATTGCAGAGGTTTTCCATTCAGGACCTTCAATATTTATTCCTTATGCATCTAGCTTCAGGCACGAGGATGTGGTATCTGGTACATTGTTGTCCCCTGCAGAAGTATACTGGCATGATTCCACATCCTTTGTAGACCAAATCAGAGAAATTCATCATCAGTGCAGCTCAGCAGGTGTTACTCATGGCTCATTGATTAAGATGCTTTGCAATTTCTACCCAGGCCTTCATGACTTCTTTGTTAACGTCTGTGGAGTCCATGAGATCCCTCCTCTCCGAAGCTACCTTCAGATTTTGCTTCATTTATCAAATGTTGCTTTGCCTTCACAAGCAGCTAGTGCAGTTTTTCAAGTTTTCCTGAAGTGGACTGATGGACTAAAATCAGGATTGAGTGCTGAGGATGTTCTTTATTTGAAAGATTCGCTTACAAAGATTGAATGTACGGTGCTTCCTACTTTACAAGATACGTGGGTTTCTCTACATCCGTCATTTGGTCTTGTGTGCTGGTGTGATGATAAGAAATTAAGCAAGCACTTTAAGCACTTGGATGGCATTGATTTTATATACTTGGGTGAACTGAGTAAAGATGATGAAGAGATGCTATGTATGAAAGTGTCCATCCTCATGCAGACTTTAGGGATTCCTGCTCTTTCAGAGGTTGTAACTCGTGAGGCAATATATTCTGGTATCGAAGACTGTAGCCTTAAAGCTGCATTGCTGGATTGGGCTCTTCCATATGCTCAGCGATACTTGCATGGTGTACACCCAGATAAATATTCTCAACTCAAGCAGTCTGAATTTGAGATGCTGAACTGTCTGCAAGTAGTAGTTGTTGAAAAGCTGTTTTACCGGAATGTTATAAAGGCTACTGGAAGTGAATCCAAGAAGCAAGTTGAATGTAGTTGCCTTCTGACGGGTAGCATTTTGTATACAACCCAAGAGTCGGATTCTCATGCATTATTTCTGGAGCTTTCTCGTTTATTTTTTAATGGAAGTCCTGAACTGCACTTGGCAAATTTCCTTCATATGATCACAACCATGGCAGAATCAGGTTCAAATGAGGAGCAGACTGAGTTTTTCATCTTAAATAGCCAAAAGGTACCAAAGCTTCCTGATGGTGAATCTGTTTGGTCCTTGTCTGGCTCTGAGGCTAACTTGGCAATGCATATCCAGTTTGATCCTAGTAGTTTAGGTCTTGTGTCTGATGCTCCTGACCTTGGTTCGTCTAATTTTAGCAATAGAGATCGGCTTAGATTTGGTACACCCAATTCGACACAAGCAAGTTTAACTGGGCGGCTAGGTGAGCTTGTAGCTTTTAAATTTTTTGTTGACAAAGCTGGCGAGGCAGTTGTTAAATGGGTTAATGAACACAACGAAACTGGGCTACCATATGACATTGTTCTGGGAGAGAAGAAAAATAGTAGAGAGTACATTGAAGTTAAAACAACCAAGTCTACAAGAAAAGACTGGTTTGAAATATCGATGAGAGAGTTGCAATTTGCGGTTGAGAAAGGTGAAGCTTTCAGTATTGCACATGTCGTTTTATTGGATAATAATGTTGCAAGGGTCTCAGTATACAAAAATCTTGCAAAATTGTGCCAGCTTCACAAACTACATTTGGCTGTCTTGATGCCTGAGCAGCAGAAGGAATTTTCCATTGTGTCCCAGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

601

Amino Acids

67.5

Weight (kDa)

5.51

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOV_C PF13020 477 - 562 7.8e-20 Protein NO VEIN, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 989
AarI CACCTGC 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 293
Acc65I GGTACC 1 cut(s) 1228
AccB1I GGYRCC 1 cut(s) 1228
AccI GTMKAC 5 cut(s) 237, 264, 1058, 1583, 1704
AciI CCGC 2 cut(s) 1423, 1631
AclWI GGATC 2 cut(s) 388, 1301
AcsI RAATTY 7 cut(s) 56, 86, 279, 926, 1144, 1448, 1778
AcuI CTGAAG 4 cut(s) 53, 172, 401, 509
AfaI GTAC 6 cut(s) 214, 571, 891, 1230, 1390, 1559
AfiI CCNNNNNNNGG 5 cut(s) 72, 620, 1042, 1072, 1346
AflIII ACRYGT 1 cut(s) 1663
AjnI CCWGG 2 cut(s) 60, 349
Alw26I GTCTC 1 cut(s) 1702
AlwI GGATC 2 cut(s) 388, 1301
AoxI GGCC 2 cut(s) 63, 352
ApeKI GCWGC 5 cut(s) 116, 294, 461, 839, 1768
ApoI RAATTY 7 cut(s) 56, 86, 279, 926, 1144, 1448, 1778
AseI ATTAAT 1 cut(s) 98
Asp718I GGTACC 1 cut(s) 1228
AspA2I CCTAGG 1 cut(s) 70
AspS9I GGNCC 3 cut(s) 28, 155, 1260
AsuHPI GGTGA 4 cut(s) 713, 1259, 1442, 1655
AvaII GGWCC 3 cut(s) 28, 155, 1260
AvrII CCTAGG 1 cut(s) 70
BaeI ACNNNNGTAYC 2 cut(s) 585, 618
BanI GGYRCC 1 cut(s) 1228
BanII GRGCYC 1 cut(s) 859
BarI GAAGNNNNNNTAC 2 cut(s) 1221, 1253
BauI CACGAG 2 cut(s) 194, 795
BbsI GAAGAC 1 cut(s) 828
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 5 cut(s) 103, 306, 473, 826, 1780
BccI CCATC 4 cut(s) 494, 671, 755, 1238
BciT130I CCWGG 2 cut(s) 62, 351
BclI TGATCA 1 cut(s) 1158
BcoDI GTCTC 1 cut(s) 1702
BfaI CTAG 5 cut(s) 71, 183, 465, 1311, 1427
BfmI CTRYAG 2 cut(s) 228, 826
BfuAI ACCTGC 1 cut(s) 293
BisI GCNGC 6 cut(s) 117, 295, 462, 840, 1424, 1769
BlnI CCTAGG 1 cut(s) 70
BlpI GCTNAGC 2 cut(s) 298, 870
BlsI GCNGC 6 cut(s) 118, 296, 463, 841, 1425, 1770
Bme1390I CCNGG 2 cut(s) 62, 351
Bme18I GGWCC 3 cut(s) 28, 155, 1260
BmgT120I GGNCC 3 cut(s) 28, 155, 1260
BmiI GGNNCC 1 cut(s) 1230
BmrFI CCNGG 2 cut(s) 62, 351
BmrI ACTGGG 3 cut(s) 1428, 1518, 1789
BmsI GCATC 7 cut(s) 7, 188, 321, 717, 924, 1324, 1749
BmuI ACTGGG 3 cut(s) 1428, 1518, 1789
BpiI GAAGAC 1 cut(s) 828
BpmI CTGGAG 2 cut(s) 44, 1115
Bpu10I CCTNAGC 2 cut(s) 525, 1764
Bpu1102I GCTNAGC 2 cut(s) 298, 870
BpuEI CTTGAG 1 cut(s) 899
Bsa29I ATCGAT 1 cut(s) 1610
BsaAI YACGTR 1 cut(s) 597
BsaI GGTCTC 1 cut(s) 1702
BsaJI CCNNGG 4 cut(s) 70, 349, 1167, 1345
BsaWI WCCGGW 1 cut(s) 978
Bsc4I CCNNNNNNNGG 5 cut(s) 72, 620, 1042, 1072, 1346
Bse1I ACTGG 7 cut(s) 245, 1003, 1300, 1423, 1513, 1601, 1795
Bse3DI GCAATG 2 cut(s) 842, 1296
BseBI CCWGG 2 cut(s) 62, 351
BseCI ATCGAT 1 cut(s) 1610
BseDI CCNNGG 4 cut(s) 70, 349, 1167, 1345
BseGI GGATG 6 cut(s) 205, 254, 535, 610, 682, 747
BseLI CCNNNNNNNGG 5 cut(s) 72, 620, 1042, 1072, 1346
BseMI GCAATG 2 cut(s) 842, 1296
BseMII CTCAG 8 cut(s) 312, 516, 698, 884, 1191, 1266, 1713, 1755
BseNI ACTGG 7 cut(s) 245, 1003, 1300, 1423, 1513, 1601, 1795
BseRI GAGGAG 2 cut(s) 390, 1205
BseXI GCAGC 5 cut(s) 103, 306, 473, 826, 1780
BsgI GTGCAG 3 cut(s) 313, 489, 1118
BshFI GGCC 2 cut(s) 65, 354
BshNI GGYRCC 1 cut(s) 1228
BshVI ATCGAT 1 cut(s) 1610
BsiSI CCGG 1 cut(s) 979
BslFI GGGAC 2 cut(s) 208, 1777
BslI CCNNNNNNNGG 5 cut(s) 72, 620, 1042, 1072, 1346
BsmAI GTCTC 1 cut(s) 1702
BsmFI GGGAC 2 cut(s) 208, 1777
BsnI GGCC 2 cut(s) 65, 354
Bso31I GGTCTC 1 cut(s) 1702
Bsp1286I GDGCHC 1 cut(s) 859
Bsp1407I TGTACA 1 cut(s) 889
Bsp143I GATC 4 cut(s) 393, 1158, 1306, 1372
Bsp1720I GCTNAGC 2 cut(s) 298, 870
Bsp19I CCATGG 1 cut(s) 1167
BspACI CCGC 2 cut(s) 1423, 1631
BspANI GGCC 2 cut(s) 65, 354
BspCNI CTCAG 8 cut(s) 311, 517, 699, 883, 1192, 1267, 1712, 1756
BspDI ATCGAT 1 cut(s) 1610
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 1 cut(s) 1230
BspMAI CTGCAG 1 cut(s) 232
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 2 cut(s) 388, 1301
BspQI GCTCTTC 1 cut(s) 864
BspT107I GGYRCC 1 cut(s) 1228
BspTNI GGTCTC 1 cut(s) 1702
BsrDI GCAATG 2 cut(s) 842, 1296
BsrGI TGTACA 1 cut(s) 889
BsrI ACTGG 7 cut(s) 245, 1003, 1300, 1423, 1513, 1601, 1795
BssECI CCNNGG 4 cut(s) 70, 349, 1167, 1345
BssMI GATC 4 cut(s) 393, 1158, 1306, 1372
BssNAI GTATAC 3 cut(s) 238, 1059, 1705
BssSI CACGAG 2 cut(s) 194, 795
BssT1I CCWWGG 3 cut(s) 70, 1167, 1345
Bst1107I GTATAC 3 cut(s) 238, 1059, 1705
Bst2BI CACGAG 2 cut(s) 194, 795
Bst2UI CCWGG 2 cut(s) 62, 351
Bst4CI ACNGT 3 cut(s) 574, 827, 944
Bst6I CTCTTC 2 cut(s) 717, 864
BstAPI GCANNNNNTGC 1 cut(s) 467
BstAUI TGTACA 1 cut(s) 889
BstBAI YACGTR 1 cut(s) 597
BstC8I GCNNGC 4 cut(s) 67, 659, 1468, 1730
BstDEI CTNAG 9 cut(s) 298, 525, 707, 870, 1200, 1275, 1379, 1699, 1764
BstDSI CCRYGG 1 cut(s) 1167
BstF5I GGATG 6 cut(s) 205, 254, 535, 610, 682, 747
BstKTI GATC 4 cut(s) 396, 1161, 1309, 1375
BstMAI GTCTC 1 cut(s) 1702
BstMBI GATC 4 cut(s) 393, 1158, 1306, 1372
BstMWI GCNNNNNNNGC 7 cut(s) 467, 667, 880, 1286, 1432, 1472, 1768
BstNI CCWGG 2 cut(s) 62, 351
BstNSI RCATGY 1 cut(s) 1667
BstSCI CCNGG 2 cut(s) 60, 349
BstSFI CTRYAG 2 cut(s) 228, 826
BstV1I GCAGC 5 cut(s) 103, 306, 473, 826, 1780
BstV2I GAAGAC 1 cut(s) 828
BstX2I RGATCY 1 cut(s) 393
BstYI RGATCY 1 cut(s) 393
BstZ17I GTATAC 3 cut(s) 238, 1059, 1705
Bsu15I ATCGAT 1 cut(s) 1610
BsuRI GGCC 2 cut(s) 65, 354
BsuTUI ATCGAT 1 cut(s) 1610
BtgI CCRYGG 1 cut(s) 1167
BtsCI GGATG 6 cut(s) 205, 254, 535, 610, 682, 747
BtsIMutI CAGTG 1 cut(s) 296
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 4 cut(s) 67, 659, 1468, 1730
CciI TCATGA 1 cut(s) 358
Cfr13I GGNCC 3 cut(s) 28, 155, 1260
ClaI ATCGAT 1 cut(s) 1610
Csp6I GTAC 6 cut(s) 213, 570, 890, 1229, 1389, 1558
CspCI CAANNNNNGTGG 4 cut(s) 241, 276, 578, 613
CviAII CATG 9 cut(s) 245, 314, 359, 389, 754, 884, 1082, 1168, 1664
CviQI GTAC 6 cut(s) 213, 570, 890, 1229, 1389, 1558
DdeI CTNAG 9 cut(s) 298, 525, 707, 870, 1200, 1275, 1379, 1699, 1764
DpnI GATC 4 cut(s) 395, 1160, 1308, 1374
DpnII GATC 4 cut(s) 393, 1158, 1306, 1372
DraI TTTAAA 1 cut(s) 1447
Eam1104I CTCTTC 2 cut(s) 717, 864
EarI CTCTTC 2 cut(s) 717, 864
Eco130I CCWWGG 3 cut(s) 70, 1167, 1345
Eco147I AGGCCT 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 859
Eco31I GGTCTC 1 cut(s) 1702
Eco47I GGWCC 3 cut(s) 28, 155, 1260
Eco57I CTGAAG 4 cut(s) 53, 172, 401, 509
EcoO109I RGGNCCY 2 cut(s) 28, 155
EcoRI GAATTC 1 cut(s) 56
EcoRII CCWGG 2 cut(s) 60, 349
EcoT14I CCWWGG 3 cut(s) 70, 1167, 1345
EcoT22I ATGCAT 3 cut(s) 181, 1087, 1296
EcoT38I GRGCYC 1 cut(s) 859
ErhI CCWWGG 3 cut(s) 70, 1167, 1345
FaeI CATG 9 cut(s) 248, 317, 362, 392, 757, 887, 1085, 1171, 1667
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 2 cut(s) 208, 1777
FatI CATG 9 cut(s) 244, 313, 358, 388, 753, 883, 1081, 1167, 1663
FauNDI CATATG 3 cut(s) 865, 1155, 1519
FbaI TGATCA 1 cut(s) 1158
FblI GTMKAC 5 cut(s) 237, 264, 1058, 1583, 1704
Fnu4HI GCNGC 6 cut(s) 117, 295, 462, 840, 1424, 1769
FokI GGATG 6 cut(s) 212, 241, 542, 597, 689, 734
FriOI GRGCYC 1 cut(s) 859
Fsp4HI GCNGC 6 cut(s) 117, 295, 462, 840, 1424, 1769
FspBI CTAG 5 cut(s) 71, 183, 465, 1311, 1427
GluI GCNGC 6 cut(s) 117, 295, 462, 840, 1424, 1769
GsuI CTGGAG 2 cut(s) 44, 1115
HaeIII GGCC 2 cut(s) 65, 354
HapII CCGG 1 cut(s) 979
Hin1II CATG 9 cut(s) 248, 317, 362, 392, 757, 887, 1085, 1171, 1667
HincII GTYRAC 2 cut(s) 373, 1459
HindII GTYRAC 2 cut(s) 373, 1459
HindIII AAGCTT 2 cut(s) 1235, 1647
HinfI GANTC 9 cut(s) 248, 384, 548, 769, 1007, 1070, 1076, 1175, 1250
HpaI GTTAAC 1 cut(s) 373
HpaII CCGG 1 cut(s) 979
HphI GGTGA 4 cut(s) 713, 1259, 1442, 1655
Hpy188I TCNGA 9 cut(s) 275, 407, 420, 784, 925, 1041, 1075, 1276, 1333
HpyAV CCTTC 7 cut(s) 168, 365, 425, 462, 1046, 1160, 1768
HpyCH4III ACNGT 3 cut(s) 574, 827, 944
HpyCH4IV ACGT 2 cut(s) 375, 596
HpyF10VI GCNNNNNNNGC 7 cut(s) 467, 667, 880, 1286, 1432, 1472, 1768
HpyF3I CTNAG 9 cut(s) 298, 525, 707, 870, 1200, 1275, 1379, 1699, 1764
HpySE526I ACGT 2 cut(s) 375, 596
Hsp92II CATG 9 cut(s) 248, 317, 362, 392, 757, 887, 1085, 1171, 1667
KpnI GGTACC 1 cut(s) 1232
Ksp22I TGATCA 1 cut(s) 1158
KspAI GTTAAC 1 cut(s) 373
Kzo9I GATC 4 cut(s) 393, 1158, 1306, 1372
LguI GCTCTTC 1 cut(s) 864
LmnI GCTCC 3 cut(s) 1096, 1192, 1342
Lsp1109I GCAGC 5 cut(s) 103, 306, 473, 826, 1780
LweI GCATC 7 cut(s) 7, 188, 321, 717, 924, 1324, 1749
MaeI CTAG 5 cut(s) 71, 183, 465, 1311, 1427
MaeII ACGT 2 cut(s) 375, 596
MaeIII GTNAC 2 cut(s) 307, 790
MalI GATC 4 cut(s) 395, 1160, 1308, 1374
MboI GATC 4 cut(s) 393, 1158, 1306, 1372
MboII GAAGA 4 cut(s) 734, 833, 851, 1552
MflI RGATCY 1 cut(s) 393
MhlI GDGCHC 1 cut(s) 859
MlyI GAGTC 2 cut(s) 393, 1079
MmeI TCCRAC 1 cut(s) 1053
Mph1103I ATGCAT 3 cut(s) 181, 1087, 1296
MslI CAYNNNNRTG 4 cut(s) 198, 675, 752, 1166
MspI CCGG 1 cut(s) 979
MspR9I CCNGG 2 cut(s) 62, 351
MvaI CCWGG 2 cut(s) 62, 351
MwoI GCNNNNNNNGC 7 cut(s) 467, 667, 880, 1286, 1432, 1472, 1768
NcoI CCATGG 1 cut(s) 1167
NdeI CATATG 3 cut(s) 865, 1155, 1519
NdeII GATC 4 cut(s) 393, 1158, 1306, 1372
NlaIII CATG 9 cut(s) 248, 317, 362, 392, 757, 887, 1085, 1171, 1667
NlaIV GGNNCC 1 cut(s) 1230
NsiI ATGCAT 3 cut(s) 181, 1087, 1296
NspI RCATGY 1 cut(s) 1667
PagI TCATGA 1 cut(s) 358
PaqCI CACCTGC 1 cut(s) 293
PceI AGGCCT 1 cut(s) 354
PciI ACATGT 1 cut(s) 1663
PciSI GCTCTTC 1 cut(s) 864
PfeI GAWTC 7 cut(s) 248, 548, 769, 1007, 1076, 1175, 1250
PkrI GCNGC 6 cut(s) 118, 296, 463, 841, 1425, 1770
PleI GAGTC 2 cut(s) 392, 1078
PpsI GAGTC 2 cut(s) 392, 1078
Ppu21I YACGTR 1 cut(s) 597
PpuMI RGGWCCY 2 cut(s) 28, 155
PscI ACATGT 1 cut(s) 1663
PshBI ATTAAT 1 cut(s) 98
PsiI TTATAA 1 cut(s) 989
Psp5II RGGWCCY 2 cut(s) 28, 155
Psp6I CCWGG 2 cut(s) 60, 349
PspGI CCWGG 2 cut(s) 60, 349
PspN4I GGNNCC 1 cut(s) 1230
PspPI GGNCC 3 cut(s) 28, 155, 1260
PspPPI RGGWCCY 2 cut(s) 28, 155
PstI CTGCAG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 393
RsaI GTAC 6 cut(s) 214, 571, 891, 1230, 1390, 1559
RsaNI GTAC 6 cut(s) 213, 570, 890, 1229, 1389, 1558
RseI CAYNNNNRTG 4 cut(s) 198, 675, 752, 1166
SapI GCTCTTC 1 cut(s) 864
SatI GCNGC 6 cut(s) 117, 295, 462, 840, 1424, 1769
Sau3AI GATC 4 cut(s) 393, 1158, 1306, 1372
Sau96I GGNCC 3 cut(s) 28, 155, 1260
SchI GAGTC 2 cut(s) 393, 1079
ScrFI CCNGG 2 cut(s) 62, 351
SduI GDGCHC 1 cut(s) 859
SfaNI GCATC 7 cut(s) 7, 188, 321, 717, 924, 1324, 1749
SfcI CTRYAG 2 cut(s) 228, 826
SinI GGWCC 3 cut(s) 28, 155, 1260
SmiMI CAYNNNNRTG 4 cut(s) 198, 675, 752, 1166
SmlI CTYRAG 1 cut(s) 914
SmoI CTYRAG 1 cut(s) 914
SseBI AGGCCT 1 cut(s) 354
SsiI CCGC 2 cut(s) 1423, 1631
SspI AATATT 2 cut(s) 165, 905
SspMI CTAG 5 cut(s) 71, 183, 465, 1311, 1427
StuI AGGCCT 1 cut(s) 354
StyD4I CCNGG 2 cut(s) 60, 349
StyI CCWWGG 3 cut(s) 70, 1167, 1345
TaaI ACNGT 3 cut(s) 574, 827, 944
TaiI ACGT 2 cut(s) 378, 599
TaqI TCGA 3 cut(s) 819, 1400, 1610
TatI WGTACW 2 cut(s) 889, 1557
TauI GCSGC 1 cut(s) 1426
TfiI GAWTC 7 cut(s) 248, 548, 769, 1007, 1076, 1175, 1250
TscAI CASTG 1 cut(s) 296
TseI GCWGC 5 cut(s) 116, 294, 461, 839, 1768
TspDTI ATGAA 9 cut(s) 123, 272, 347, 419, 735, 752, 1142, 1198, 1509
TspGWI ACGGA 2 cut(s) 140, 603
TspRI CASTG 1 cut(s) 296
VpaK11BI GGWCC 3 cut(s) 28, 155, 1260
VspI ATTAAT 1 cut(s) 98
XapI RAATTY 7 cut(s) 56, 86, 279, 926, 1144, 1448, 1778
XceI RCATGY 1 cut(s) 1667
XmaJI CCTAGG 1 cut(s) 70
XmiI GTMKAC 5 cut(s) 237, 264, 1058, 1583, 1704
XspI CTAG 5 cut(s) 71, 183, 465, 1311, 1427
Zsp2I ATGCAT 3 cut(s) 181, 1087, 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.