Rh4BG184800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000582
Physical Location & Seq
Forward (+)
32355135 .. 32375430
20296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG184800.1

Sequence Viewer

Length: 8118 bp
ATGCCTGAGGACCCGAGAGAGGTGCTCGAGAAGATCGACCGTGCGGTGGAGAAGCTTCGCGACGAGCTTGTCGCCGCCGGGAAGAACGTCTCGGCCTGGAAGGTGTTACAGTCTGCGCTTCTGATGCTCAATGTTGATGCCGAGACTTGTTTGGGCCTCAAAGTTGAGCAAGTGCCGTCTCTTCACCGCCTCATGGTCACTGAAGGAAGGATAAATGCATTTATCCATTGCTTTGTTGGGGTTCGAACAATTACATCTTTGTATGATTTGGAAGTTGCAATCTGCAAGAATGAGGATATTGAGCAGTTTGAAGAGCTTGGATTGGGTCCTTTGTTGAAACACCCACTAGTTTTGCAATATTTCTCGGTGGATTCTGAAACAACTGAAGTCTTTAAGATATCTAGTGAGGAGATACTACATTTACTCAGTAGGTACCTAAAGAAGCCGAAAGTTAAGGAAAATTCTGTTGAGGGGTTCTTGGACTTCATTGTTACGAAGCGACCTGTTGCAGGCAAGGAAAAGCTTGGAATTCGAATATTTAGTTTGGGGGTGCATGCTTCTGCTATGCGTGAAGCCAAGGCTTCAGAACGTGCAGCTTTATGGAAATCATTACAGGAATTAAAACGAAAGTCTCCCAAAATATCTAGGAGGAAGGCACCAATTTTCTCCTCGCTGAAAAAGCGGCTAGATAAACATTTTCGCGCCATTTCGCAGCAATCTGGATTGTGTTCTGTTGTGAATGGAAAGGAAGATACACTTGTTTCTTCAAGATCAAAGGATGAAGGTATTAATGATTATTTACATGATGACAAAGGTAATGACCATGGTACTGGTGGTCATATTGACTCACTAAAATCCCTGAAAAGTTCTGACAAAGAGGGTAAGTGTTCTTACTTGTCTGAAGTGGAGGAGAGGTCTTCGCTCATGTCAAACCACCCTTCTCCAAGCCAAAAGCATAATGAGAGTAATGGGTCAGTTAAAAAGAAAAGAAAATATGAAAATCTAAGCTGCCCTATTTCTGTTCCCTTAAAGTTGAGCAAGAGAGAGAAGGTAGAGCAAGATGCTCTTCCTACAGAAAGTGACAGTGAAACTAAGGAGGTCAGCGAGATGCTTAAACTTGATCTTTCAATTGCCAATAATCTTTTGAGGATGTTCATTACAACTTGGAAGGAGGCATGTAGGGAGCAGCCTGTGGCTGAGGTACTTTGGGCCATGCTTCGCTCTTGTAATACAAAATATAGGAGGACAAAGAGAATCATGGCAATGTTTTCGACATACCCTTTGGTTGGATTACTCAATGTTGCCGTCTCATCTATCAAATGTGGAATGTGGGATAATATGTTTGATACGTCCCAAACCATTAGTCCACGTGAGTTGGCTAACACTGTAACTGATAATTATCCTGAACATGTCGAACAAAGTATAAAGGATGCACCACCAGTCAGCACCGAACATGCTACCGAACATGGACAAACCTCTTGCTGCCTGAAGCCAAATCATCAGGATCGTCCACGTCGGTGGAGGAAGATCCCAAGGAGTCGTAAGAAGTTGGAGCCTGAAAACGGACACATCTGCTACCTTTGTCATCAAGAGGGACATGTCAAGAGAAGATGCCCTCTGTGGCAGCAAACTAAAAAATCATCATATGAACCACAGAGTCCTCAACAATTGGCTCAGTATTCTATTTCCGGAGAAGAGGTGGAGGAGAAAGTCAGTGCTGTGGTGGACGGTGTTTCAGTTAAAGAGATAATCAGGAAAGTTGCTACATATTTTGAGACTGATCACGGAATGTCTAGCAATGGTAAATCACTTCCGGAGAAGATATTTATTTACTTCAGAAAGCTTTGGAATTGTGAGCTCTGGTTGACTGAAGAATTTTCTGTCAAGGAATTTAGATCCCTTGGTTATGGGGAATTTATGATGTTTTTAGAAAAGTATGCCCGTCTTCTGCCACCGGAGATGTGCAAGTTCTTGAAAGATGACAGCAGTGGAAAGTTTCCTTTGGAGGTTTGCATGCTTCCGCATTATTTAGTTGTTTTGGTATCTCAAGCTTTAAACAATTTATGGGAGGACCAAAAGATTACCAAACTGAACATTTCTTCTCTGCTTGGGAAGCAGTTCCCGGCCCTTAGTTTCCATACCATTGAAAATGGTTCAGTAGAAGACTTTAAAAGTATAGTAGGAAAGCATAAAGACACTGCAATTTCCAAATGTGTACTATTTTCAATGACAATATGCGGAACTAGTTACACTCTAGAATCATTGGAAAACTGTAGTAAGAATGCTGTATTGAAAAGCAGTTTGGTGAGTGTTAACAGTGGCCAGAAAATAAGCAATCAATCTGTTGCATCCAAGGAGGCAATTGCAGTCTTACTTAGGGCACCCATGATGTCAGATTTGAATTTATGGTCACATTGGGACCTCTTATTTGCTCCCTCTCTTGGCCCTCTTGTACCGTGGTTATTGAATGAAGTCAACACAGATGAATTACTCTGTTTGGTAACCAAAGATGGCAAAGTTATTCGGTTAGATCATTCAGCTACTGTAGATTCATTCTCGAAAGCTGCTCTTCAAGGATCCTCTTTTCAAACAGCAGTGAAAATGTTATCTCTCTTTTCACTGGTTGGGGGAGAAAAACATGTCCCCGTGTCCCTTTTGAAATTCCATGTACAGCGTGCATTTGAAGTTATTTTCAAGACCTATCTAGATGGTATGGAAGTATATGACAAGAAGAGTTCTCTTAATCAAGGAACAGTTTTGTGTGGACAGAAAATGGTTGTTGAAATTGCTGCTGGTAAATGCAGTAACTTACACAGAGCTGTAACGCAGACGAAAATTGCTGAGTCTGTTATATCACATTTTTTTCTTGATTGCCTGGGGTATCTACCTGCAGAGTTTCGCAGTTTTGCTGCTGATGTGTTACTTTCTGGGATGCGGTCTGTTTTTAAGCATGCGGCTTCAGCCATTTTGGATGAATGCATCCAAATAGAGCAGCGTCTCATACTTCATGAAGTTGGCTTATCTCTTGGTATAGTGGAGTGGATTAGTGATTACCATGTATTTAGTTCCTTGGATTCTACTGATTCGTTCATGTCTGAGGATTCTGGTCTCAATGCTGATAGAGATCAAATGGGGTCAGACTCAAAATATATGCAAAATGTGTCAAAAAAGTTGGCTGCTTCTGAACAGAGTATGGCTGCATCTGTTAGGGCAGTTGAGCATAAAGGAGGATGTGCTGATGTTAGCCGGATGGTTGATGGTGCAGGAGTTTCTGATGCTGGGATTGGCATTGGCATTGGTTACAGACAACATCCTACTAATATTAATGAACATGAAGATGCTTTCCAGGTTATTGAGTCAATCAGGAGAGATGAATTCGGTTTGGATTCAAGCCCATTGACCTTTGAAAGTATCGTCTTAAAGAAACAGCATGCTCGCTTAGGGAGAGCTCTGCACTGTCTTTCACAGGAATTATATTCTCAAGATTCTCATTTTCTTCTTGAGCTGGTTCAAAATGCTGATGATAATACTTATCCAACAAATATAGAACCAACTCTAGCATTCATTCTTCAAGAATCAGGTATTGTTGTTTTGAATAATGAGCAAGGCTTCTCTGCCCAGAACATTAGAGCACTTTGTGATGTTGGAAATTCAACCAAGAAAGGATCCAGTGCCGGATATATAGGGCAAAAGGGCATCGGTTTCAAATCAGTATTTCGTGTCACAGATGCTCCAGAAATTCATTCTAATGGATTTCATATCAAGTTTGATACAAGTGAGAGTCAGATTGGTTTAATTCTGCCCACAGTTGTACCTCCTTGCAATCTTGAGTTGTTTAGACAGCTGACATCTAGTGACATTGATAAACCAGATTGTTACTGCTGGAAAACTTGTATTGTTCTTCCTTTCAGATCGAAAGTATCAGATGGAACTGTCTTGAACCACATAATGAATATGTTTTCAGATCTTCATCCATCTTTACTACTCTTTCTTCACCGCCTCAAGTGTATCCGGTTTAGAAACCTAATCGATGATTCACTTACTGTCATGAGAAAAGAAATTGTAGGAGACGGTATTGTCAAGGTTTCACATGGGAATACGAAGATGACATGGTTTGTAGTATCTCAGAAATTGCACTCAGATAATCTTCGTAGTGATGTGCAAACAACAGAAATCTCAATTGCTTTTACATTGAAGGAATCGGATAAGGGCATTTATCGTCCAGATATAGGCCAACAGCCTGTTTTTGCATTTCTTCCTCTTAGAACATACGGACTGAAATTTATTGTTCAGGGTGATTTTGTTCTTCCTTCATCCAGAGAGGAAGTGGATGGAGATAGTCCCTGGAACCAATGGCTATTGTCAGAATTTCCTGGTTTATTTGTTGATGCAGAGAAATCATTTTGTTCTCTTCCATGTTTCAAAGAGAATCCTGGGACTGCTGTGGCAGCTTACATGTCCTTTGTTCCACTTGTTGGGGAAGTGCATGGTTTCTTTTCTTCTCTTCCTCGATTAATTATTTCTAAATTACGGATGTCAAATTGCTTACTTCAGGAAGGAGAAAAGAAAGAATGGGTTCCTCCTTGCAAGGTCCTAAGAAATTGGAGTAAACAGGCTCGTTCACTTCTCCCTGATGGTCTACTGCATGAGCATCTTGGCCTTGGATTCTTGGATAAAGATATAGTTCTGCCTGATCCACTTGCAAGAGTTCTAGGTATTGCAGAGTATGGGCCCACAGTTCTGCTTCAGGTAATGGCTTCTTTATGTGGTAAGCAGGATGGCCTCAAATCAATGGGAATGGGTTGGATGGCCTCTTTGCTTAGTGAACTTTATGCAATGTCATTCAAATCTTCTGTTGAAACTTCCTTTGATTCTGGAATAGAAATGGGATTCCTAGAAAAACTTCAAAAAATTCCATTCATACCTCTTTCAGATGGTACATATGGTGCAGTCGTTGAAGGCCCAATTTGGCTACAGTTTGATGCCGTAGGGACTGGTTTTGGGGATCATCATGGACTTGAATCTTTTCCAAAGTTATATGCTAAACTTCGGATTGTAAGTCCGGAGCTCTATGCATCGTCTGCTGATATGCCATCCATGGATGTGACGCCAATTGACAAAGTCATTAGTATGCTCCATAGAATTGGTGTCCAACGGTTGTCCGCACACGAGATTCTCAAGGCTCACATCTTGCCAGCTATATCTGATGATAGAATTACAGTTAGGGATGAAGATTTGATGACTGATTATATCTGCTTCGCAATGGTTCACTTACAGTCGAGCTGCTCTGATTGTCATGCTGAAAGGGAATACATAATATCAGAACTACAAAATAAAGCTTATATTCTAACTAATAATGGCTTTAAACGACCTGCTGAGGCATCAATTCATTTCAGTAAAGAATTTGGAAATCCAGTGGACATAAGTAGGTTGATTGATAGGGTAAATCTGACATGGGATGAGGTTGATATCTCCTATTTGAAGCATCCAGTTGCTAAATCACTTCCAAATGGACTGATGAAGTGGAGAGATTTTTTCCAGAAAATTGGCATTGTGGATTTTGTGAAGGTAGTTCAAGTTGAGAAGGGTTTCACTGAGTTGTCTGAGGCTCTATTTAATAATCTAACTTGGGACCAGCACACTATTTCCCATGGGTTGAATGCCACAGATTGGGAATCACCTGAGTTAGTCCATTTATTATCCCTATTGTCCAGAGATGGCAACAGAAAAGGTTGTGAGTATCTCTTAGAGGTTCTCGATAAATTATGGGATGATTGCTATAGTGACAGAGCTACAGGGTACTGCTCTTCTAAGTCTGTTGCAGATAGAAAGCCCTTCAAATCTTCATTTATGAGCACCATCTGCGATGTGCAATGGGTAGTATCAGGAATGGATGATAAACTTCACTATCCCAAGGATCTATATCACGACAGTGTTGCAGTGCGGTCAATTCTTGGTGGATCTGCCCCATTTTTTGTTCCTAAGGCTACCAGTGAAAAATTTGTATCTGATATTGGTTTTAAGACAAGAGTCTCTCTTCACGATGCTCTGGAGATGTTGAAACTATGGAGATGTGAGAATCCGTTTAGGGCCAGCCTAGCACAAATGTCCAAATTTTACTCATTAATCTGGAATGAAATGGCTTCTTCAAAGAAGACAACTGCAGAGGAATTCTGTTTGGAACCTTTTGTATTTGTTCCTTATGAATCTAGCTTCCGGCATGAGGATGTGGTGTCTGGTACATTTCTGTCCCTTGAAGAAGTATACTGGGATGATTCTACTTTCTTTGTGGACCAAATCAAGGAGATCCATCATCAGTGCAACTCAACAGTTGGTAATCATGGCCCCATAAACAGGATTCTGAGTAATTTCTACCCGGCCCTTCATGACTTCTTTGTTGATATATGTGGAGTACATGAGATCCCTCCTCTTCGTAGCTACCTTCAGATTTTGCTACAGTTTTCAAATGCTGTTTTGCCTTCACAAGCAGCTAATGCTGTTTTTCAAGTTTTTCAGAAGTGGGCTGATGGGCTCCAATCAGGAATGAGTGCTGAGGATATTGTATACTTGAAAGATTCTCTTACAAGGATGGAATGTACTGTGCTTCCTACTGTACAGGATAAGTGGGTATCTCTACATCCTTCCTATGGTCTCCTGTGCTGGTGTGATGATAAGACGTTGAAGAAGCAGTTTATGCATATGGATGGACTTGATTTTCTTTACTCCGGTGGACTAAGTAATGATGATGAAGAGATCCTAAGTACAAAAATGTCCGTCCTCATGCGAACCTTAGGGATTTCTGCACTTTCAGAGGTTGTGACTCGTGAGGCGATGTTTAATGGTCCGACAGATTCTAGCTTTAAAGCTGCATTGCTGGATTGGGCTCTGCCATATGCGCAGCGCTACTTACATAGTTTACATCCTGATAAATATTCTCAACTCAAGCAGTCTGGATTCGATATACTAAATCGTTTGCGAGTAGTAGAAGTTCAAAAGCTGTCCTACCGCAATGTTATAAAGATTGCTGGAAGTGAATCCAAGAAGCAAATCGAATGTAGTTGTGTTCTGCAGGATCACGATCTGTATACAACCCAGGAATCAGATTCTCACGCATTATTTATGGAGCTTTCTCGTTTGTTTTTTGATGGAAAACCAGAATTACACTTGGCAAATTTCCTTCATATGATCACAACAATGGCTGAATCAGGTTCAACTGAGGAGCAGATAGACTTTTTTATCTTCAATAGCCAAAAGGTGCCAAAGCTTCCTGATGGAGAATCTGTTTGGTCCCTGTCACATGTGCATAATGATAGGTTACCCCAGCCAAGTGACTGGCGCTTTGTTGGTTGGAAAGCCCCACTAAGCTTTGGTTATGCAAATCTTTTCAAGGCTCAGGCACAATTTGCACAGCTTACCAGTGTCTCACAAATTGAAATGGATAATGATTCTGAAACCCATGTCAGACAAACAGTTGAATCAACTCCCATTTCAGTCGACATTAAATGGACAATTGGTGAGGGTACAGCAACAACATCTGCACCTTTAGTGCTGCCAAACTCCAATGATTTGCAAGAACACTTTGATGATGAGACTGATATGGATACTGATTTCAATCCTAATAATCTAGATTTTGTTGTTGACCCTCGCGTTTTGGGTTCATCTGATTGTAGCAAGGTAGACCATCCTAGATATGGCTCTAGAGGAAGAGATGCAATGCGTGGAAGAGATGCAATGCTTACCGGGAGGTTAGGTGAGGTTGTTGCTTTTAAATACTTAATTGCAAAAGCTGGCAAGTCAGTTGTGAGGTGGGTTAATGAAAGAATTGAAACTGGGCTACCCTATGACATAGTTGTAGGTGAGAAGGAAGATAGTCTAGAGTTCATTGAGGTTAAAGCCACCCAACAACAAAAAAAGGATTGGTTCCACATATCAATGAGGGAGTGGCAATTTGCAGCGGAAAAAGGCGAAGCCTTCAGCATTTTGCACGTCCTCTTATTGGGTAATAATGCTGCAAGAGTCTCAGTTTACAAGAATCCTGTAAAATTGTGTCATCTGGGCAAGCTACAGTTGCATCTTTCGATGCCTAGGCAGGAGAAGGAATTATTTCTTGTGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

2705

Amino Acids

304.39

Weight (kDa)

6.05

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 1163 - 1340 9.5e-09 Sacsin domain
NOV_C PF13020 2584 - 2670 1.7e-19 Protein NO VEIN, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 7016
AasI GACNNNNNNGTC 2 cut(s) 68, 4076
Acc16I TGCGCA 1 cut(s) 6897
Acc36I ACCTGC 2 cut(s) 2896, 5380
Acc65I GGTACC 1 cut(s) 432
AccB1I GGYRCC 4 cut(s) 432, 655, 2380, 7255
AccB7I CCANNNNNTGG 2 cut(s) 4475, 5670
AccI GTMKAC 6 cut(s) 4639, 6264, 6564, 7085, 7494, 7677
AccII CGCG 3 cut(s) 60, 702, 7647
AccIII TCCGGA 3 cut(s) 1688, 1813, 5062
AcoI YGGCCR 1 cut(s) 2320
AcvI CACGTG 1 cut(s) 1370
AcyI GRCGYC 1 cut(s) 5108
AdeI CACNNNGTG 1 cut(s) 3638
AfeI AGCGCT 1 cut(s) 6902
AflIII ACRYGT 5 cut(s) 1408, 1597, 2638, 4455, 7297
AhlI ACTAGT 2 cut(s) 346, 2243
AjiI CACGTC 2 cut(s) 1514, 7987
AjnI CCWGG 7 cut(s) 95, 2874, 3345, 4343, 4372, 4432, 7092
AjuI GAANNNNNNNTTGG 2 cut(s) 2285, 2317
AleI CACNNNNGTG 2 cut(s) 1516, 5931
Alw21I GWGCWC 6 cut(s) 27, 1860, 3451, 3634, 5070, 5858
AlwNI CAGNNNCTG 1 cut(s) 2543
Ama87I CYCGRG 2 cut(s) 13, 26
Aor13HI TCCGGA 3 cut(s) 1688, 1813, 5062
Aor51HI AGCGCT 1 cut(s) 6902
ApaI GGGCCC 1 cut(s) 4734
ArsI GACNNNNNNTTYG 8 cut(s) 1264, 1296, 1348, 1380, 2411, 2443, 7322, 7354
AseI ATTAAT 4 cut(s) 789, 3324, 4514, 6125
Asp700I GAANNNNTTC 6 cut(s) 2117, 4575, 4902, 5025, 5588, 8103
Asp718I GGTACC 1 cut(s) 432
AspA2I CCTAGG 1 cut(s) 8084
AspLEI GCGC 5 cut(s) 118, 704, 6898, 6903, 7338
AsuC2I CCSGG 4 cut(s) 79, 2123, 6377, 7741
AsuHPI GGTGA 8 cut(s) 176, 2317, 3986, 4307, 5670, 7526, 7763, 7868
AsuII TTCGAA 2 cut(s) 244, 532
AvaI CYCGRG 2 cut(s) 13, 26
AvaII GGWCC 9 cut(s) 10, 326, 2071, 2419, 4591, 5632, 6292, 6842, 7287
AvrII CCTAGG 1 cut(s) 8084
AxyI CCTNAGG 3 cut(s) 6, 5982, 6790
BaeGI GKGCMC 2 cut(s) 2383, 4734
BaeI ACNNNNGTAYC 4 cut(s) 744, 777, 3795, 3828
BalI TGGCCA 1 cut(s) 2322
BamHI GGATCC 2 cut(s) 2576, 3665
BanI GGYRCC 4 cut(s) 432, 655, 2380, 7255
BanII GRGCYC 6 cut(s) 1860, 3451, 4734, 5070, 6534, 6886
BauI CACGAG 2 cut(s) 5168, 6822
BbrPI CACGTG 1 cut(s) 1370
BbsI GAAGAC 4 cut(s) 909, 1937, 2169, 6161
Bbv12I GWGCWC 6 cut(s) 27, 1860, 3451, 3634, 5070, 5858
BbvCI CCTCAGC 3 cut(s) 1197, 5376, 6552
BceAI ACGGC 3 cut(s) 160, 1289, 4970
BcgI CGANNNNNNTGC 6 cut(s) 4, 38, 1251, 1285, 5918, 5952
BciT130I CCWGG 7 cut(s) 97, 2876, 3347, 4345, 4374, 4434, 7094
BciVI GTATCC 2 cut(s) 4019, 7594
BclI TGATCA 2 cut(s) 1780, 7185
BcnI CCSGG 4 cut(s) 79, 2123, 6377, 7741
BcuI ACTAGT 2 cut(s) 346, 2243
BfoI RGCGCY 2 cut(s) 6904, 7339
BfuAI ACCTGC 2 cut(s) 2896, 5380
BfuI GTATCC 2 cut(s) 4019, 7594
BglII AGATCT 1 cut(s) 3964
BlnI CCTAGG 1 cut(s) 8084
Bme18I GGWCC 9 cut(s) 10, 326, 2071, 2419, 4591, 5632, 6292, 6842, 7287
BmeT110I CYCGRG 2 cut(s) 13, 26
BmgBI CACGTC 2 cut(s) 1514, 7987
BmrI ACTGGG 2 cut(s) 6277, 7839
BmuI ACTGGG 2 cut(s) 6277, 7839
BoxI GACNNNNGTC 1 cut(s) 6029
BpiI GAAGAC 4 cut(s) 909, 1937, 2169, 6161
BplI GAGNNNNNCTC 2 cut(s) 9, 41
BpmI CTGGAG 2 cut(s) 3717, 6071
Bpu10I CCTNAGC 5 cut(s) 1197, 3439, 5376, 6552, 7392
Bpu14I TTCGAA 2 cut(s) 244, 532
BpuEI CTTGAG 7 cut(s) 2031, 3465, 3521, 3848, 3986, 5162, 6926
BpuMI CCSGG 4 cut(s) 79, 2123, 6377, 7741
Bsa29I ATCGAT 1 cut(s) 4029
BsaAI YACGTR 1 cut(s) 1370
BsaBI GATNNNNATC 6 cut(s) 1398, 3123, 3969, 5922, 7077, 7611
BsaHI GRCGYC 1 cut(s) 5108
BsaI GGTCTC 2 cut(s) 3113, 6656
BsaWI WCCGGW 6 cut(s) 1688, 1813, 1954, 4011, 5062, 6725
Bse21I CCTNAGG 3 cut(s) 6, 5982, 6790
Bse8I GATNNNNATC 6 cut(s) 1398, 3123, 3969, 5922, 7077, 7611
BseAI TCCGGA 3 cut(s) 1688, 1813, 5062
BseBI CCWGG 7 cut(s) 97, 2876, 3347, 4345, 4374, 4434, 7094
BseCI ATCGAT 1 cut(s) 4029
BseJI GATNNNNATC 6 cut(s) 1398, 3123, 3969, 5922, 7077, 7611
BseRI GAGGAG 6 cut(s) 422, 658, 923, 1718, 6417, 7232
BseSI GKGCMC 2 cut(s) 2383, 4734
BseYI CCCAGC 2 cut(s) 3278, 7320
BsgI GTGCAG 6 cut(s) 612, 3282, 3437, 4968, 6786, 7521
Bsh1236I CGCG 3 cut(s) 60, 702, 7647
Bsh1285I CGRYCG 1 cut(s) 40
BshNI GGYRCC 4 cut(s) 432, 655, 2380, 7255
BshVI ATCGAT 1 cut(s) 4029
BsiEI CGRYCG 1 cut(s) 40
BsiHKAI GWGCWC 6 cut(s) 27, 1860, 3451, 3634, 5070, 5858
BsiHKCI CYCGRG 2 cut(s) 13, 26
BsmBI CGTCTC 5 cut(s) 94, 183, 1312, 3002, 4062
BsmI GAATGC 4 cut(s) 2287, 2981, 3560, 5665
Bso31I GGTCTC 2 cut(s) 3113, 6656
BsoBI CYCGRG 2 cut(s) 13, 26
Bsp119I TTCGAA 2 cut(s) 244, 532
Bsp120I GGGCCC 1 cut(s) 4730
Bsp13I TCCGGA 3 cut(s) 1688, 1813, 5062
Bsp1407I TGTACA 2 cut(s) 2668, 6613
Bsp19I CCATGG 3 cut(s) 823, 5097, 5650
Bsp68I TCGCGA 1 cut(s) 60
BspDI ATCGAT 1 cut(s) 4029
BspEI TCCGGA 3 cut(s) 1688, 1813, 5062
BspFNI CGCG 3 cut(s) 60, 702, 7647
BspHI TCATGA 3 cut(s) 3007, 4047, 6385
BspMAI CTGCAG 3 cut(s) 2893, 6166, 7071
BspMI ACCTGC 2 cut(s) 2896, 5380
BspQI GCTCTTC 4 cut(s) 306, 1071, 2574, 5812
BspT104I TTCGAA 2 cut(s) 244, 532
BspT107I GGYRCC 4 cut(s) 432, 655, 2380, 7255
BspTNI GGTCTC 2 cut(s) 3113, 6656
BsrGI TGTACA 2 cut(s) 2668, 6613
BssNAI GTATAC 3 cut(s) 6265, 6565, 7086
BssNI GRCGYC 1 cut(s) 5108
BssSI CACGAG 2 cut(s) 5168, 6822
Bst1107I GTATAC 3 cut(s) 6265, 6565, 7086
Bst2BI CACGAG 2 cut(s) 5168, 6822
Bst2UI CCWGG 7 cut(s) 97, 2876, 3347, 4345, 4374, 4434, 7094
BstACI GRCGYC 1 cut(s) 5108
BstAPI GCANNNNNTGC 5 cut(s) 5081, 5862, 6494, 6694, 7403
BstAUI TGTACA 2 cut(s) 2668, 6613
BstBAI YACGTR 1 cut(s) 1370
BstBI TTCGAA 2 cut(s) 244, 532
BstDSI CCRYGG 4 cut(s) 823, 2456, 5097, 5650
BstEII GGTNACC 2 cut(s) 2500, 7314
BstENI CCTNNNNNAGG 1 cut(s) 507
BstFNI CGCG 3 cut(s) 60, 702, 7647
BstH2I RGCGCY 2 cut(s) 6904, 7339
BstHHI GCGC 5 cut(s) 118, 704, 6898, 6903, 7338
BstMCI CGRYCG 1 cut(s) 40
BstNI CCWGG 7 cut(s) 97, 2876, 3347, 4345, 4374, 4434, 7094
BstPAI GACNNNNGTC 1 cut(s) 6029
BstPI GGTNACC 2 cut(s) 2500, 7314
BstSLI GKGCMC 2 cut(s) 2383, 4734
BstUI CGCG 3 cut(s) 60, 702, 7647
BstV2I GAAGAC 4 cut(s) 909, 1937, 2169, 6161
BstXI CCANNNNNNTGG 5 cut(s) 830, 1518, 5144, 5546, 7332
BstZ17I GTATAC 3 cut(s) 6265, 6565, 7086
Bsu15I ATCGAT 1 cut(s) 4029
Bsu36I CCTNAGG 3 cut(s) 6, 5982, 6790
BsuI GTATCC 2 cut(s) 4019, 7594
BsuTUI ATCGAT 1 cut(s) 4029
BtgI CCRYGG 4 cut(s) 823, 2456, 5097, 5650
BtgZI GCGATG 2 cut(s) 5880, 6845
BtrI CACGTC 2 cut(s) 1514, 7987
BtsI GCAGTG 4 cut(s) 1993, 2196, 2601, 5946
BtuMI TCGCGA 1 cut(s) 60
BveI ACCTGC 2 cut(s) 2896, 5380
CaiI CAGNNNCTG 1 cut(s) 2543
CciI TCATGA 3 cut(s) 3007, 4047, 6385
CfoI GCGC 5 cut(s) 118, 704, 6898, 6903, 7338
ClaI ATCGAT 1 cut(s) 4029
CseI GACGC 2 cut(s) 2984, 5116
CspCI CAANNNNNGTGG 4 cut(s) 333, 368, 1356, 1391
DraI TTTAAA 5 cut(s) 2055, 2170, 5365, 6862, 7768
DraIII CACNNNGTG 1 cut(s) 3638
DrdI GACNNNNNNGTC 2 cut(s) 68, 4076
DseDI GACNNNNNNGTC 2 cut(s) 68, 4076
EaeI YGGCCR 1 cut(s) 2320
Ecl136II GAGCTC 3 cut(s) 1858, 3449, 5068
Eco24I GRGCYC 6 cut(s) 1860, 3451, 4734, 5070, 6534, 6886
Eco31I GGTCTC 2 cut(s) 3113, 6656
Eco32I GATATC 2 cut(s) 399, 5470
Eco47I GGWCC 9 cut(s) 10, 326, 2071, 2419, 4591, 5632, 6292, 6842, 7287
Eco47III AGCGCT 1 cut(s) 6902
Eco53kI GAGCTC 3 cut(s) 1858, 3449, 5068
Eco72I CACGTG 1 cut(s) 1370
Eco81I CCTNAGG 3 cut(s) 6, 5982, 6790
Eco88I CYCGRG 2 cut(s) 13, 26
Eco91I GGTNACC 2 cut(s) 2500, 7314
EcoICRI GAGCTC 3 cut(s) 1858, 3449, 5068
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoO109I RGGNCCY 4 cut(s) 10, 326, 2419, 4591
EcoO65I GGTNACC 2 cut(s) 2500, 7314
EcoRI GAATTC 3 cut(s) 528, 3374, 6170
EcoRII CCWGG 7 cut(s) 95, 2874, 3345, 4343, 4372, 4432, 7092
EcoRV GATATC 2 cut(s) 399, 5470
EcoT22I ATGCAT 4 cut(s) 220, 2981, 5077, 6699
EcoT38I GRGCYC 6 cut(s) 1860, 3451, 4734, 5070, 6534, 6886
Esp3I CGTCTC 5 cut(s) 94, 183, 1312, 3002, 4062
FalI AAGNNNNNCTT 8 cut(s) 741, 773, 1050, 1082, 2553, 2585, 6564, 6596
FauNDI CATATG 5 cut(s) 1645, 4942, 6699, 6892, 7182
FbaI TGATCA 2 cut(s) 1780, 7185
FblI GTMKAC 6 cut(s) 4639, 6264, 6564, 7085, 7494, 7677
FriOI GRGCYC 6 cut(s) 1860, 3451, 4734, 5070, 6534, 6886
FspI TGCGCA 1 cut(s) 6897
GlaI GCGC 5 cut(s) 117, 703, 6897, 6902, 7337
GsaI CCCAGC 2 cut(s) 3282, 7324
GsuI CTGGAG 2 cut(s) 3717, 6071
HaeII RGCGCY 2 cut(s) 6904, 7339
HgaI GACGC 2 cut(s) 2984, 5116
HhaI GCGC 5 cut(s) 118, 704, 6898, 6903, 7338
Hin1I GRCGYC 1 cut(s) 5108
Hin6I GCGC 5 cut(s) 116, 702, 6896, 6901, 7336
HinP1I GCGC 5 cut(s) 116, 702, 6896, 6901, 7336
HincII GTYRAC 5 cut(s) 1866, 2314, 2476, 7495, 7639
HindII GTYRAC 5 cut(s) 1866, 2314, 2476, 7495, 7639
HindIII AAGCTT 7 cut(s) 53, 521, 1841, 2049, 5337, 7262, 7363
HpaI GTTAAC 1 cut(s) 2314
HphI GGTGA 8 cut(s) 176, 2317, 3986, 4307, 5670, 7526, 7763, 7868
Hpy99I CGWCG 2 cut(s) 65, 1518
HpyCH4IV ACGT 7 cut(s) 87, 589, 1349, 1369, 1513, 6677, 7986
HpySE526I ACGT 7 cut(s) 87, 589, 1349, 1369, 1513, 6677, 7986
Hsp92I GRCGYC 1 cut(s) 5108
HspAI GCGC 5 cut(s) 116, 702, 6896, 6901, 7336
Kpn2I TCCGGA 3 cut(s) 1688, 1813, 5062
KpnI GGTACC 1 cut(s) 436
Ksp22I TGATCA 2 cut(s) 1780, 7185
KspAI GTTAAC 1 cut(s) 2314
LguI GCTCTTC 4 cut(s) 306, 1071, 2574, 5812
LmnI GCTCC 9 cut(s) 1183, 1552, 2437, 3736, 5065, 5139, 6537, 7123, 7219
MaeII ACGT 7 cut(s) 87, 589, 1349, 1369, 1513, 6677, 7986
MfeI CAATTG 6 cut(s) 1128, 1667, 2361, 4179, 5112, 7509
MlsI TGGCCA 1 cut(s) 2322
MluNI TGGCCA 1 cut(s) 2322
MmeI TCCRAC 8 cut(s) 1267, 1530, 3560, 3625, 4784, 5176, 6869, 7328
Mox20I TGGCCA 1 cut(s) 2322
Mph1103I ATGCAT 4 cut(s) 220, 2981, 5077, 6699
MroI TCCGGA 3 cut(s) 1688, 1813, 5062
MroXI GAANNNNTTC 6 cut(s) 2117, 4575, 4902, 5025, 5588, 8103
MscI TGGCCA 1 cut(s) 2322
Msp20I TGGCCA 1 cut(s) 2322
MspA1I CMGCKG 2 cut(s) 3844, 7955
MteI GCGCNGCGC 1 cut(s) 6899
MunI CAATTG 6 cut(s) 1128, 1667, 2361, 4179, 5112, 7509
Mva1269I GAATGC 4 cut(s) 2287, 2981, 3560, 5665
MvaI CCWGG 7 cut(s) 97, 2876, 3347, 4345, 4374, 4434, 7094
MvnI CGCG 3 cut(s) 60, 702, 7647
NciI CCSGG 4 cut(s) 79, 2123, 6377, 7741
NcoI CCATGG 3 cut(s) 823, 5097, 5650
NdeI CATATG 5 cut(s) 1645, 4942, 6699, 6892, 7182
NmeAIII GCCGAG 2 cut(s) 71, 166
NruI TCGCGA 1 cut(s) 60
NsbI TGCGCA 1 cut(s) 6897
NsiI ATGCAT 4 cut(s) 220, 2981, 5077, 6699
NspV TTCGAA 2 cut(s) 244, 532
OliI CACNNNNGTG 2 cut(s) 1516, 5931
PaeI GCATGC 4 cut(s) 557, 2017, 2954, 3434
PaeR7I CTCGAG 1 cut(s) 26
PagI TCATGA 3 cut(s) 3007, 4047, 6385
PciI ACATGT 5 cut(s) 1408, 1597, 2638, 4455, 7297
PciSI GCTCTTC 4 cut(s) 306, 1071, 2574, 5812
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PctI GAATGC 4 cut(s) 2287, 2981, 3560, 5665
PdmI GAANNNNTTC 6 cut(s) 2117, 4575, 4902, 5025, 5588, 8103
PflFI GACNNNGTC 1 cut(s) 5120
PflMI CCANNNNNTGG 2 cut(s) 4475, 5670
PmaCI CACGTG 1 cut(s) 1370
PmlI CACGTG 1 cut(s) 1370
Ppu21I YACGTR 1 cut(s) 1370
PpuMI RGGWCCY 4 cut(s) 10, 326, 2419, 4591
PscI ACATGT 5 cut(s) 1408, 1597, 2638, 4455, 7297
PshAI GACNNNNGTC 1 cut(s) 6029
PshBI ATTAAT 4 cut(s) 789, 3324, 4514, 6125
PsiI TTATAA 1 cut(s) 7016
Psp124BI GAGCTC 3 cut(s) 1860, 3451, 5070
Psp5II RGGWCCY 4 cut(s) 10, 326, 2419, 4591
Psp6I CCWGG 7 cut(s) 95, 2874, 3345, 4343, 4372, 4432, 7092
PspCI CACGTG 1 cut(s) 1370
PspEI GGTNACC 2 cut(s) 2500, 7314
PspFI CCCAGC 2 cut(s) 3278, 7320
PspGI CCWGG 7 cut(s) 95, 2874, 3345, 4343, 4372, 4432, 7092
PspOMI GGGCCC 1 cut(s) 4730
PspPPI RGGWCCY 4 cut(s) 10, 326, 2419, 4591
PsrI GAACNNNNNNTAC 2 cut(s) 6972, 7004
PstI CTGCAG 3 cut(s) 2893, 6166, 7071
PstNI CAGNNNCTG 1 cut(s) 2543
PsyI GACNNNGTC 1 cut(s) 5120
PvuII CAGCTG 1 cut(s) 3844
RruI TCGCGA 1 cut(s) 60
SacI GAGCTC 3 cut(s) 1860, 3451, 5070
SalI GTCGAC 1 cut(s) 7493
SapI GCTCTTC 4 cut(s) 306, 1071, 2574, 5812
Sfr274I CTCGAG 1 cut(s) 26
SfuI TTCGAA 2 cut(s) 244, 532
SinI GGWCC 9 cut(s) 10, 326, 2071, 2419, 4591, 5632, 6292, 6842, 7287
SlaI CTCGAG 1 cut(s) 26
SmlI CTYRAG 8 cut(s) 26, 2046, 3480, 3500, 3827, 4001, 5177, 6941
SmoI CTYRAG 8 cut(s) 26, 2046, 3480, 3500, 3827, 4001, 5177, 6941
SpeI ACTAGT 2 cut(s) 346, 2243
SphI GCATGC 4 cut(s) 557, 2017, 2954, 3434
SspI AATATT 4 cut(s) 359, 537, 3322, 6932
SstI GAGCTC 3 cut(s) 1860, 3451, 5070
TaiI ACGT 7 cut(s) 90, 592, 1352, 1372, 1516, 6680, 7989
TatI WGTACW 6 cut(s) 2215, 2668, 6412, 6596, 6613, 6761
TauI GCSGC 3 cut(s) 77, 685, 2957
TspGWI ACGGA 6 cut(s) 1578, 1800, 4287, 4546, 6072, 6763
Tth111I GACNNNGTC 1 cut(s) 5120
Van91I CCANNNNNTGG 2 cut(s) 4475, 5670
VpaK11BI GGWCC 9 cut(s) 10, 326, 2071, 2419, 4591, 5632, 6292, 6842, 7287
VspI ATTAAT 4 cut(s) 789, 3324, 4514, 6125
XagI CCTNNNNNAGG 1 cut(s) 507
XbaI TCTAGA 5 cut(s) 2254, 2704, 7624, 7697, 7873
XcmI CCANNNNNNNNNTGG 4 cut(s) 233, 830, 4324, 7688
XhoI CTCGAG 1 cut(s) 26
XmaJI CCTAGG 1 cut(s) 8084
XmiI GTMKAC 6 cut(s) 4639, 6264, 6564, 7085, 7494, 7677
XmnI GAANNNNTTC 6 cut(s) 2117, 4575, 4902, 5025, 5588, 8103
Zsp2I ATGCAT 4 cut(s) 220, 2981, 5077, 6699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.