Rmu_sc0004087.1_g000027

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004087.1
OG0000582
Physical Location & Seq
Forward (+)
92443 .. 102689
10247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004087.1_g000027.1.cds

Sequence Viewer

Length: 5037 bp
atgtacggacgtcctcctcgctcctccggcggcgcatggggtcagccaccgccgcttgcacaacctctaaactctaatttctcactccaaaaccccaacactccatacatctaccctataaaccctgcctttacgccccaccatgcctaccgaaacttctcccccaacaatctcccaccgcagaaccctagcttcgttccccagcagttctcgaattcgccgtttcggcctcctccgcctcagggtaactccaaagagctgctggagaaagtcgaccgcgccgtggcgaaggctcggtcggaccttgtcgccgccggcgacagcgtctcggcgtggaaggtctcgcagtcggcgcttctgatgctccagatcgatggctggagctctctggggtttcagatgcagcaggtgccgtctctacaccgcctcatgttcactgaaggcaagataaatgcatttattcagtgttttgttgcggttcgaagaatttcttcattatacgatctggaagtagcagtctgcaagaatgagggcatcgagaagtttgaagagctagggttgggccctttggtgcgacacccacttgttttgcattattactcagtgaagcctgatactaatgaagtttataagattactagcgatgagataatatctttactctctgagtacatggatacctgcaagaataaagaaattaagattgaagaatttttagatttcattgtaaagaagcgatctgttgcaagcaaggaagatcttggaatacgaattcagagcatggggatgcatatttctgctatccgggatgtgaagaaaactgaacaggttcttaagcaaacatctaagaaaggtaacaataagcgttacttctcattgaaaaggcagctagacgaacgtttcaacgacatttctcagcgtgttgaatcattttcctccgtacagaagttttgtggcaagcacattaggtttgattcttcaaaagactcggaggctgacagtagtgatgataattcatatgaagatgatgatgaagttaatgaccattcgacaggcaatcaggttaacttattatcaaaatctgttacaacttctgatcgagctagcagatgtccttacccttctgaattagaagagaagaaacggctaggattgaaccagcattccccagctagttgctcccaaaagcttggtgagagtaatcagtcagctaaaaagaaaagaaactctgaagatgtaaacagtccaatctcagtgcccaccaaattgcgtaagagagataagacgggagaagatgctcctcgaacagggcatggctggaaaaccaatgaggttaacaatttggatgaaaatgatctcgcaattactgatagctgtttaaagaccttcattacaacttggaaggaggcatgtcggcagaaaactgtggctgaggtacttgatagattactgcagcttaataacatagaagctcagaagaaaaaggaaatcaagtcaatgttttctttggatccattaattggattgttaaatgttgctgtctcatctatcaaatccggattgtgggatagcatgtatgatacattccaaactgttggtcagttaactgacagtcttccagataactgtcctgaatatgtgaacatagatgttgaaccaagtattaaagatgtgaaaagtaccaaggatgcaccagtcattcctgaacatgctgtagaccacaggcagagtgtttctgttgaggacataatcaggaaacttacaatgtattttgagattgatcgaggtgtacatggcaatggtaaatcacttcaggagaaaatatgtaccttcttgacaaagctttacagttgtgaactgtggttagtggaagaattttctgtcaaggaatttagatcacttggtcatggagagtttttaaggttcttagaaaattatgctggtctacttccacaagacctgtgcaagttcctgactgatgatgtaattgagaagtgccctttggaagtttgcatgctccagcaccatttggttgttttactatctcaagctttaaatagtttatgggaggatgcaaatatcacaaaacaggacattctgttgctgcttaggaagcaattcccattggttagtttcaaaactattgaaaatggttcagtagaagattttttaagcatagtaggaaagcataaaaatgctgtgatttccaaatgtgttctgttctcaatggcattacatggcactagctatgcgatagactcattagtacattatgagaacgtcctattgaaaagcatgtcattgagttctgacagtggccagaaagcaaaaacccatgttacatccaaggatgcaattaaagtcttagctagggcaccaatgatgtctgacttaaacttatggtcacactgggaccttttgtttgctccttcacttggtcctcttgtaccatggttattaaatgaagttaacacagatgaattgttgtgtttggtgaccaaagatggaaaagtctttcggttagaccaatcagctactgtagattccttcgtggaagctgcccttcatggatcctctttccaaacagcagtgaaattgttatctctgttttcactagttgggggagaaaaacatgtccctgtgcctcttttgaaaatccacatgcagcatgcatttgaagtcattttgaaaaattttgtagatgacatggtagtacgtgacaataagaattctcttaattatggaaaagcgttgtctggacaacaaatgattggtgaaattgcagctggtaaactatcacacagagacttacacaagacagatattggaaaacccattatatcaagatttgtccttgaatgtcttgggtatttacctgcagaatttcgtggttttgctgctgatgtattgctttcgggaatgcagtctgttgtcaaacatgctgcttcggccatattgagcgagtgcagccaatcagagcaacgtatcatgcttcatgaagttggtttgtctcttggtatagttgagtggattaatgattactacacatttctttccaatgatacgggtcagtcgttcatatctggggcctcatgttccaatgctgttggatatgaaatggggtcaggttcaaagcccttgcaagatgtttcagataagtttttgacttctggagggagcatggctcgatctgttgtggaagatgtgcaggaagttggatgtactgatgtctctctgaaaattggtggtgcggaaatctctgacgagagagctggctgtggttacacacaacattctgccaaaattagtgaatatgaagatgcatcccaggtgattgagtcaatcagaagagatgaatttggtcttcattcaagccaaacgacctctgaaagtatcatgttgaagaagcaccatgctcgcttagggagagctctacattgtctttcacaggaattgtattctcaggattcacattttcttcttgagctggttcaaaatgcagatgataatacatacccaacatatgtagaaccaactttaacattcattcttcaagactcgggtattattgttttaaataatgagcaaggcttctctgccgagaacattagagcactttgtgatgttggaagttcaaccaagaaaggatctaatgctggctatattgggcggaagggcattggtttcaaatctgtgtttcgggtcacagatgctccagaaattcattctaatggattccatatcaagtttgatataagtcagggtcagattggttttcttctgccaacagtagtacctccttgcaacattgacatgttaagtaggctgacttctagtgacagcgatcaacaggacagtaacttctggaacacttgcattgttcttcctttcagatcgaagttttcagatggatctgtcatgaaaagcattataaatatgttttcagatcttcatccatctttattactcttccttcaccgcctccaatgtataaagtttagaaacttgcttgataattcactgactgtcatgagaaaagaaactgtgggagatggtatagtaaaggtttcacatgggaaagagaaaatgacatggttcttagtatctcagaaattgcaagcggattttatacgtagtgatgtacaaacaacagaaatctccatagcatttacattgaaggaattagataatggtgattatggaccagatctagggcaacagcctgcttttgcatttcttcctctaagaacctatggcctgaagtttattgttcagggtgattttgttcttccttcatctagagaggaagtggatggagatagtccctggaaccagtggctattatcagaatttcctggtttatttgtcaatgctgagaggtctttttgttctcttccttgctttagagagaaccccgggagagcagtggcagcttatatgtcatttgttccacttgttggggaagtgcatgggtttttttcttctcttcctcgattaatcatttctaaattacggatgtcaaattgcttactcctggaaggaggaaataacgagtgggttcctccttgcaaggtcctaagaggttggaatgagcaggcccgattgcttctgcctgatagtttacttcgggagcatcttggccttgggttgttggataaaaatatagttctacctgattcacttgcaagagcacttggcattgcagactatggacccaaagttctgcttcaagttatggactccttatgtcgtttgcaaaatggcctcacatcaatgggactgggttggttgacctcttggctaagtgaactgtatgcaatgtcattcaatgcttctgtggaaacatcatttgactctgaacttggaatggatttgatagaagaactccgaaaaattccattcattcctctttcagatggtacatatggtgcagtagacaaagacccaatttggttacattttgatgccttgagcactgggtttgagggtcagcatggacttgagtcttttcctaacttgtatgctaatcttcgtatt
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

1679

Amino Acids

187.31

Weight (kDa)

5.81

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 630, 3932
AarI CACCTGC 1 cut(s) 397
AatII GACGTC 1 cut(s) 13
Acc36I ACCTGC 3 cut(s) 397, 689, 2884
AccB1I GGYRCC 2 cut(s) 409, 2374
AccB7I CCANNNNNTGG 2 cut(s) 1520, 4457
AccI GTMKAC 4 cut(s) 273, 1716, 1945, 4935
AccII CGCG 1 cut(s) 279
AccIII TCCGGA 1 cut(s) 1556
AclI AACGTT 1 cut(s) 898
AclWI GGATC 6 cut(s) 1505, 1518, 2565, 2578, 3655, 3919
AcoI YGGCCR 2 cut(s) 2317, 2949
AcuI CTGAAG 4 cut(s) 459, 1251, 1796, 4280
AcyI GRCGYC 1 cut(s) 10
AdeI CACNNNGTG 1 cut(s) 3620
AfiI CCNNNNNNNGG 8 cut(s) 242, 283, 1307, 1520, 1563, 2435, 4211, 4457
AflII CTTAAG 1 cut(s) 833
AflIII ACRYGT 2 cut(s) 2632, 3813
AhlI ACTAGT 1 cut(s) 2614
AjnI CCWGG 4 cut(s) 3327, 4325, 4354, 4533
AjuI GAANNNNNNNTTGG 2 cut(s) 1985, 2017
AloI GAACNNNNNNTCC 4 cut(s) 1148, 1180, 4704, 4736
Alw21I GWGCWC 5 cut(s) 386, 3433, 3616, 4693, 4976
Alw26I GTCTC 7 cut(s) 331, 346, 420, 1546, 2799, 3015, 3235
AlwI GGATC 6 cut(s) 1505, 1518, 2565, 2578, 3655, 3919
AlwNI CAGNNNCTG 1 cut(s) 2537
Ama87I CYCGRG 2 cut(s) 3559, 4415
Aor13HI TCCGGA 1 cut(s) 1556
AoxI GGCC 9 cut(s) 227, 562, 2317, 2949, 3087, 4255, 4596, 4639, 4762
ApaI GGGCCC 1 cut(s) 566
ArsI GACNNNNNNTTYG 6 cut(s) 281, 313, 1582, 1614, 2405, 2437
AseI ATTAAT 3 cut(s) 1517, 3033, 4496
Asp700I GAANNNNTTC 4 cut(s) 487, 490, 828, 2117
AspLEI GCGC 3 cut(s) 35, 281, 355
AsuC2I CCSGG 3 cut(s) 806, 4416, 4417
AsuHPI GGTGA 7 cut(s) 1205, 2506, 2786, 3343, 3968, 4205, 4289
AsuII TTCGAA 1 cut(s) 481
AsuNHI GCTAGC 1 cut(s) 1103
AvaI CYCGRG 2 cut(s) 3559, 4415
AvaII GGWCC 6 cut(s) 301, 2413, 2438, 4202, 4573, 4712
AxyI CCTNAGG 1 cut(s) 240
BaeGI GKGCMC 4 cut(s) 566, 1260, 2000, 2377
BaeI ACNNNNGTAYC 2 cut(s) 3777, 3810
BalI TGGCCA 1 cut(s) 2319
BamHI GGATCC 2 cut(s) 1510, 2570
BanI GGYRCC 2 cut(s) 409, 2374
BanII GRGCYC 3 cut(s) 386, 566, 3433
BarI GAAGNNNNNNTAC 2 cut(s) 3814, 3846
BbsI GAAGAC 2 cut(s) 1607, 3356
Bbv12I GWGCWC 5 cut(s) 386, 3433, 3616, 4693, 4976
BbvCI CCTCAGC 1 cut(s) 1431
BccI CCATC 7 cut(s) 368, 2498, 3902, 3964, 4046, 4307, 4910
BceAI ACGGC 4 cut(s) 205, 266, 397, 1160
BcgI CGANNNNNNTGC 2 cut(s) 3398, 3432
BciT130I CCWGG 4 cut(s) 3329, 4327, 4356, 4535
BciVI GTATCC 1 cut(s) 670
BcnI CCSGG 3 cut(s) 806, 4416, 4417
BcoDI GTCTC 7 cut(s) 331, 346, 420, 1546, 2799, 3015, 3235
BcuI ACTAGT 1 cut(s) 2614
BfmI CTRYAG 4 cut(s) 1451, 1713, 2538, 2877
BfoI RGCGCY 1 cut(s) 356
BfrI CTTAAG 1 cut(s) 833
BfuAI ACCTGC 3 cut(s) 397, 689, 2884
BfuI GTATCC 1 cut(s) 670
BglI GCCNNNNNGGC 1 cut(s) 226
BglII AGATCT 3 cut(s) 757, 3946, 4207
Bme1390I CCNGG 7 cut(s) 806, 3329, 4327, 4356, 4416, 4417, 4535
Bme18I GGWCC 6 cut(s) 301, 2413, 2438, 4202, 4573, 4712
BmeT110I CYCGRG 2 cut(s) 3559, 4415
BmrFI CCNGG 7 cut(s) 806, 3329, 4327, 4356, 4416, 4417, 4535
BmrI ACTGGG 3 cut(s) 2419, 4790, 4986
BmtI GCTAGC 1 cut(s) 1107
BmuI ACTGGG 3 cut(s) 2419, 4790, 4986
BoxI GACNNNNGTC 1 cut(s) 5002
BpiI GAAGAC 2 cut(s) 1607, 3356
BplI GAGNNNNNCTC 2 cut(s) 3169, 3201
BpmI CTGGAG 6 cut(s) 284, 350, 400, 2003, 3192, 3699
Bpu10I CCTNAGC 3 cut(s) 1431, 2108, 3421
Bpu14I TTCGAA 1 cut(s) 481
BpuEI CTTGAG 4 cut(s) 2031, 3503, 4990, 5021
BpuMI CCSGG 3 cut(s) 806, 4416, 4417
Bsa29I ATCGAT 1 cut(s) 372
BsaAI YACGTR 2 cut(s) 2717, 4133
BsaBI GATNNNNATC 1 cut(s) 3951
BsaHI GRCGYC 1 cut(s) 10
BsaI GGTCTC 1 cut(s) 346
BsaJI CCNNGG 9 cut(s) 282, 1683, 2346, 2450, 3327, 4325, 4414, 4415, 4642
BsaWI WCCGGW 1 cut(s) 1556
BsaXI ACNNNNNCTCC 2 cut(s) 3697, 3727
Bsc4I CCNNNNNNNGG 8 cut(s) 242, 283, 1307, 1520, 1563, 2435, 4211, 4457
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 5 cut(s) 1694, 2414, 4333, 4785, 4981
Bse21I CCTNAGG 1 cut(s) 240
Bse3DI GCAATG 3 cut(s) 1804, 4698, 4824
Bse8I GATNNNNATC 1 cut(s) 3951
BseAI TCCGGA 1 cut(s) 1556
BseBI CCWGG 4 cut(s) 3329, 4327, 4356, 4535
BseCI ATCGAT 1 cut(s) 372
BseDI CCNNGG 9 cut(s) 282, 1683, 2346, 2450, 3327, 4325, 4414, 4415, 4642
BseJI GATNNNNATC 1 cut(s) 3951
BseLI CCNNNNNNNGG 8 cut(s) 242, 283, 1307, 1520, 1563, 2435, 4211, 4457
BseMI GCAATG 3 cut(s) 1804, 4698, 4824
BseNI ACTGG 5 cut(s) 1694, 2414, 4333, 4785, 4981
BseRI GAGGAG 4 cut(s) 6, 13, 222, 1290
BseSI GKGCMC 4 cut(s) 566, 1260, 2000, 2377
BseYI CCCAGC 2 cut(s) 201, 1168
BsgI GTGCAG 3 cut(s) 2986, 3227, 4950
Bsh1236I CGCG 1 cut(s) 279
Bsh1285I CGRYCG 2 cut(s) 277, 299
BshFI GGCC 9 cut(s) 229, 564, 2319, 2951, 3089, 4257, 4598, 4641, 4764
BshNI GGYRCC 2 cut(s) 409, 2374
BshVI ATCGAT 1 cut(s) 372
BsiEI CGRYCG 2 cut(s) 277, 299
BsiHKAI GWGCWC 5 cut(s) 386, 3433, 3616, 4693, 4976
BsiHKCI CYCGRG 2 cut(s) 3559, 4415
BsiSI CCGG 5 cut(s) 27, 315, 805, 1557, 4416
BslFI GGGAC 4 cut(s) 2426, 2621, 4308, 4791
BslI CCNNNNNNNGG 8 cut(s) 242, 283, 1307, 1520, 1563, 2435, 4211, 4457
BsmAI GTCTC 7 cut(s) 331, 346, 420, 1546, 2799, 3015, 3235
BsmBI CGTCTC 2 cut(s) 331, 420
BsmFI GGGAC 4 cut(s) 2426, 2621, 4308, 4791
BsmI GAATGC 2 cut(s) 1162, 2925
BsnI GGCC 9 cut(s) 229, 564, 2319, 2951, 3089, 4257, 4598, 4641, 4764
Bso31I GGTCTC 1 cut(s) 346
BsoBI CYCGRG 2 cut(s) 3559, 4415
Bsp119I TTCGAA 1 cut(s) 481
Bsp120I GGGCCC 1 cut(s) 562
Bsp1286I GDGCHC 9 cut(s) 386, 566, 1260, 2000, 2377, 3433, 3616, 4693, 4976
Bsp13I TCCGGA 1 cut(s) 1556
Bsp1407I TGTACA 2 cut(s) 1789, 4141
Bsp19I CCATGG 1 cut(s) 2450
BspANI GGCC 9 cut(s) 229, 564, 2319, 2951, 3089, 4257, 4598, 4641, 4764
BspDI ATCGAT 1 cut(s) 372
BspEI TCCGGA 1 cut(s) 1556
BspFNI CGCG 1 cut(s) 279
BspHI TCATGA 3 cut(s) 2995, 3918, 4029
BspMAI CTGCAG 2 cut(s) 1455, 2881
BspMI ACCTGC 3 cut(s) 397, 689, 2884
BspOI GCTAGC 1 cut(s) 1107
BspPI GGATC 6 cut(s) 1505, 1518, 2565, 2578, 3655, 3919
BspQI GCTCTTC 1 cut(s) 543
BspT104I TTCGAA 1 cut(s) 481
BspT107I GGYRCC 2 cut(s) 409, 2374
BspTI CTTAAG 1 cut(s) 833
BspTNI GGTCTC 1 cut(s) 346
BsrDI GCAATG 3 cut(s) 1804, 4698, 4824
BsrFI RCCGGY 1 cut(s) 314
BsrGI TGTACA 2 cut(s) 1789, 4141
BsrI ACTGG 5 cut(s) 1694, 2414, 4333, 4785, 4981
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 9 cut(s) 282, 1683, 2346, 2450, 3327, 4325, 4414, 4415, 4642
BssNI GRCGYC 1 cut(s) 10
BssT1I CCWWGG 4 cut(s) 1683, 2346, 2450, 4642
Bst2UI CCWGG 4 cut(s) 3329, 4327, 4356, 4535
Bst6I CTCTTC 6 cut(s) 543, 1128, 3343, 3974, 4398, 4491
BstACI GRCGYC 1 cut(s) 10
BstAFI CTTAAG 1 cut(s) 833
BstAPI GCANNNNNTGC 1 cut(s) 409
BstAUI TGTACA 2 cut(s) 1789, 4141
BstBAI YACGTR 2 cut(s) 2717, 4133
BstBI TTCGAA 1 cut(s) 481
BstDSI CCRYGG 2 cut(s) 282, 2450
BstEII GGTNACC 1 cut(s) 2494
BstENI CCTNNNNNAGG 1 cut(s) 1305
BstFNI CGCG 1 cut(s) 279
BstH2I RGCGCY 1 cut(s) 356
BstHHI GCGC 3 cut(s) 35, 281, 355
BstMAI GTCTC 7 cut(s) 331, 346, 420, 1546, 2799, 3015, 3235
BstMCI CGRYCG 2 cut(s) 277, 299
BstNI CCWGG 4 cut(s) 3329, 4327, 4356, 4535
BstPAI GACNNNNGTC 1 cut(s) 5002
BstPI GGTNACC 1 cut(s) 2494
BstSCI CCNGG 7 cut(s) 804, 3327, 4325, 4354, 4414, 4415, 4533
BstSFI CTRYAG 4 cut(s) 1451, 1713, 2538, 2877
BstSLI GKGCMC 4 cut(s) 566, 1260, 2000, 2377
BstSNI TACGTA 1 cut(s) 4133
BstUI CGCG 1 cut(s) 279
BstV2I GAAGAC 2 cut(s) 1607, 3356
BstX2I RGATCY 7 cut(s) 757, 1510, 2570, 3647, 3911, 3946, 4207
BstXI CCANNNNNNTGG 3 cut(s) 374, 1190, 1595
BstYI RGATCY 7 cut(s) 757, 1510, 2570, 3647, 3911, 3946, 4207
Bsu15I ATCGAT 1 cut(s) 372
Bsu36I CCTNAGG 1 cut(s) 240
BsuI GTATCC 1 cut(s) 670
BsuRI GGCC 9 cut(s) 229, 564, 2319, 2951, 3089, 4257, 4598, 4641, 4764
BsuTUI ATCGAT 1 cut(s) 372
BtgI CCRYGG 2 cut(s) 282, 2450
BtgZI GCGATG 1 cut(s) 657
BtsI GCAGTG 2 cut(s) 2595, 4431
BveI ACCTGC 3 cut(s) 397, 689, 2884
CaiI CAGNNNCTG 1 cut(s) 2537
CciI TCATGA 3 cut(s) 2995, 3918, 4029
CfoI GCGC 3 cut(s) 35, 281, 355
Cfr10I RCCGGY 1 cut(s) 314
Cfr9I CCCGGG 1 cut(s) 4415
ClaI ATCGAT 1 cut(s) 372
CseI GACGC 1 cut(s) 313
CspCI CAANNNNNGTGG 2 cut(s) 570, 605
DraI TTTAAA 3 cut(s) 1380, 2055, 3576
DraIII CACNNNGTG 1 cut(s) 3620
EaeI YGGCCR 2 cut(s) 2317, 2949
Eam1104I CTCTTC 6 cut(s) 543, 1128, 3343, 3974, 4398, 4491
EarI CTCTTC 6 cut(s) 543, 1128, 3343, 3974, 4398, 4491
EciI GGCGGA 2 cut(s) 225, 3685
Ecl136II GAGCTC 2 cut(s) 384, 3431
Eco105I TACGTA 1 cut(s) 4133
Eco130I CCWWGG 4 cut(s) 1683, 2346, 2450, 4642
Eco24I GRGCYC 3 cut(s) 386, 566, 3433
Eco31I GGTCTC 1 cut(s) 346
Eco47I GGWCC 6 cut(s) 301, 2413, 2438, 4202, 4573, 4712
Eco53kI GAGCTC 2 cut(s) 384, 3431
Eco57I CTGAAG 4 cut(s) 459, 1251, 1796, 4280
Eco81I CCTNAGG 1 cut(s) 240
Eco88I CYCGRG 2 cut(s) 3559, 4415
Eco91I GGTNACC 1 cut(s) 2494
EcoICRI GAGCTC 2 cut(s) 384, 3431
EcoNI CCTNNNNNAGG 1 cut(s) 1305
EcoO109I RGGNCCY 4 cut(s) 563, 2413, 3087, 4573
EcoO65I GGTNACC 1 cut(s) 2494
EcoRI GAATTC 3 cut(s) 214, 771, 2728
EcoRII CCWGG 4 cut(s) 3327, 4325, 4354, 4533
EcoT14I CCWWGG 4 cut(s) 1683, 2346, 2450, 4642
EcoT22I ATGCAT 4 cut(s) 457, 792, 2674, 3325
EcoT38I GRGCYC 3 cut(s) 386, 566, 3433
ErhI CCWWGG 4 cut(s) 1683, 2346, 2450, 4642
Esp3I CGTCTC 2 cut(s) 331, 420
FalI AAGNNNNNCTT 8 cut(s) 475, 507, 854, 886, 2547, 2579, 4710, 4742
FaqI GGGAC 4 cut(s) 2426, 2621, 4308, 4791
FauNDI CATATG 3 cut(s) 1018, 3523, 4924
FblI GTMKAC 4 cut(s) 273, 1716, 1945, 4935
FriOI GRGCYC 3 cut(s) 386, 566, 3433
GlaI GCGC 3 cut(s) 34, 280, 354
GsaI CCCAGC 2 cut(s) 205, 1172
GsuI CTGGAG 6 cut(s) 284, 350, 400, 2003, 3192, 3699
HaeII RGCGCY 1 cut(s) 356
HaeIII GGCC 9 cut(s) 229, 564, 2319, 2951, 3089, 4257, 4598, 4641, 4764
HapII CCGG 5 cut(s) 27, 315, 805, 1557, 4416
HgaI GACGC 1 cut(s) 313
HhaI GCGC 3 cut(s) 35, 281, 355
Hin1I GRCGYC 1 cut(s) 10
Hin6I GCGC 3 cut(s) 33, 279, 353
HinP1I GCGC 3 cut(s) 33, 279, 353
HincII GTYRAC 6 cut(s) 274, 1066, 1336, 1605, 2470, 4791
HindII GTYRAC 6 cut(s) 274, 1066, 1336, 1605, 2470, 4791
HindIII AAGCTT 3 cut(s) 1187, 1841, 2049
HpaI GTTAAC 4 cut(s) 1066, 1336, 1605, 2470
HpaII CCGG 5 cut(s) 27, 315, 805, 1557, 4416
HphI GGTGA 7 cut(s) 1205, 2506, 2786, 3343, 3968, 4205, 4289
HpyCH4IV ACGT 6 cut(s) 10, 898, 2280, 2716, 2983, 4132
HpySE526I ACGT 6 cut(s) 10, 898, 2280, 2716, 2983, 4132
Hsp92I GRCGYC 1 cut(s) 10
HspAI GCGC 3 cut(s) 33, 279, 353
Kpn2I TCCGGA 1 cut(s) 1556
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
KspAI GTTAAC 4 cut(s) 1066, 1336, 1605, 2470
LguI GCTCTTC 1 cut(s) 543
MaeII ACGT 6 cut(s) 10, 898, 2280, 2716, 2983, 4132
MflI RGATCY 7 cut(s) 757, 1510, 2570, 3647, 3911, 3946, 4207
MhlI GDGCHC 9 cut(s) 386, 566, 1260, 2000, 2377, 3433, 3616, 4693, 4976
MlsI TGGCCA 1 cut(s) 2319
MluNI TGGCCA 1 cut(s) 2319
MlyI GAGTC 7 cut(s) 980, 2252, 3347, 3551, 4733, 4847, 5012
MmeI TCCRAC 6 cut(s) 279, 3088, 3196, 3607, 4565, 4632
Mox20I TGGCCA 1 cut(s) 2319
Mph1103I ATGCAT 4 cut(s) 457, 792, 2674, 3325
MreI CGCCGGCG 1 cut(s) 314
MroI TCCGGA 1 cut(s) 1556
MroNI GCCGGC 1 cut(s) 314
MroXI GAANNNNTTC 4 cut(s) 487, 490, 828, 2117
MscI TGGCCA 1 cut(s) 2319
MslI CAYNNNNRTG 7 cut(s) 785, 1797, 2193, 3729, 3812, 4772, 4962
Msp20I TGGCCA 1 cut(s) 2319
MspA1I CMGCKG 1 cut(s) 2786
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 5 cut(s) 27, 315, 805, 1557, 4416
MspR9I CCNGG 7 cut(s) 806, 3329, 4327, 4356, 4416, 4417, 4535
Mva1269I GAATGC 2 cut(s) 1162, 2925
MvaI CCWGG 4 cut(s) 3329, 4327, 4356, 4535
MvnI CGCG 1 cut(s) 279
NaeI GCCGGC 1 cut(s) 316
NciI CCSGG 3 cut(s) 806, 4416, 4417
NcoI CCATGG 1 cut(s) 2450
NdeI CATATG 3 cut(s) 1018, 3523, 4924
NgoMIV GCCGGC 1 cut(s) 314
NheI GCTAGC 1 cut(s) 1103
NmeAIII GCCGAG 2 cut(s) 308, 3625
NmuCI GTSAC 5 cut(s) 2403, 2494, 2717, 3703, 3836
NsiI ATGCAT 4 cut(s) 457, 792, 2674, 3325
NspV TTCGAA 1 cut(s) 481
PaeI GCATGC 2 cut(s) 2017, 2672
PagI TCATGA 3 cut(s) 2995, 3918, 4029
PaqCI CACCTGC 1 cut(s) 397
PciI ACATGT 2 cut(s) 2632, 3813
PciSI GCTCTTC 1 cut(s) 543
PcsI WCGNNNNNNNCGW 4 cut(s) 218, 279, 315, 3071
PctI GAATGC 2 cut(s) 1162, 2925
PdiI GCCGGC 1 cut(s) 316
PdmI GAANNNNTTC 4 cut(s) 487, 490, 828, 2117
PfeI GAWTC 6 cut(s) 926, 974, 2543, 3467, 3735, 4676
PflFI GACNNNGTC 2 cut(s) 305, 323
PflMI CCANNNNNTGG 2 cut(s) 1520, 4457
PfoI TCCNGGA 2 cut(s) 804, 4533
PleI GAGTC 7 cut(s) 980, 2252, 3346, 3551, 4733, 4847, 5011
PpsI GAGTC 7 cut(s) 980, 2252, 3346, 3551, 4733, 4847, 5011
Ppu21I YACGTR 2 cut(s) 2717, 4133
PpuMI RGGWCCY 2 cut(s) 2413, 4573
PscI ACATGT 2 cut(s) 2632, 3813
PshAI GACNNNNGTC 1 cut(s) 5002
PshBI ATTAAT 3 cut(s) 1517, 3033, 4496
PsiI TTATAA 2 cut(s) 630, 3932
Psp124BI GAGCTC 2 cut(s) 386, 3433
Psp1406I AACGTT 1 cut(s) 898
Psp5II RGGWCCY 2 cut(s) 2413, 4573
Psp6I CCWGG 4 cut(s) 3327, 4325, 4354, 4533
PspEI GGTNACC 1 cut(s) 2494
PspFI CCCAGC 2 cut(s) 201, 1168
PspGI CCWGG 4 cut(s) 3327, 4325, 4354, 4533
PspOMI GGGCCC 1 cut(s) 562
PspPPI RGGWCCY 2 cut(s) 2413, 4573
PstI CTGCAG 2 cut(s) 1455, 2881
PstNI CAGNNNCTG 1 cut(s) 2537
PsuI RGATCY 7 cut(s) 757, 1510, 2570, 3647, 3911, 3946, 4207
PsyI GACNNNGTC 2 cut(s) 305, 323
PvuII CAGCTG 1 cut(s) 2786
RseI CAYNNNNRTG 7 cut(s) 785, 1797, 2193, 3729, 3812, 4772, 4962
SacI GAGCTC 2 cut(s) 386, 3433
SalI GTCGAC 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 543
SchI GAGTC 7 cut(s) 980, 2252, 3347, 3551, 4733, 4847, 5012
ScrFI CCNGG 7 cut(s) 806, 3329, 4327, 4356, 4416, 4417, 4535
SduI GDGCHC 9 cut(s) 386, 566, 1260, 2000, 2377, 3433, 3616, 4693, 4976
SfcI CTRYAG 4 cut(s) 1451, 1713, 2538, 2877
SfuI TTCGAA 1 cut(s) 481
SgrAI CRCCGGYG 1 cut(s) 314
SinI GGWCC 6 cut(s) 301, 2413, 2438, 4202, 4573, 4712
SmaI CCCGGG 1 cut(s) 4417
SmiMI CAYNNNNRTG 7 cut(s) 785, 1797, 2193, 3729, 3812, 4772, 4962
SmlI CTYRAG 5 cut(s) 833, 2046, 3482, 4969, 5000
SmoI CTYRAG 5 cut(s) 833, 2046, 3482, 4969, 5000
SnaBI TACGTA 1 cut(s) 4133
SpeI ACTAGT 1 cut(s) 2614
SphI GCATGC 2 cut(s) 2017, 2672
SstI GAGCTC 2 cut(s) 386, 3433
StyD4I CCNGG 7 cut(s) 804, 3327, 4325, 4354, 4414, 4415, 4533
StyI CCWWGG 4 cut(s) 1683, 2346, 2450, 4642
TaiI ACGT 6 cut(s) 13, 901, 2283, 2719, 2986, 4135
TaqII GACCGA 1 cut(s) 285
TatI WGTACW 5 cut(s) 669, 1789, 2266, 3221, 4141
TauI GCSGC 3 cut(s) 33, 55, 314
TfiI GAWTC 6 cut(s) 926, 974, 2543, 3467, 3735, 4676
TseFI GTSAC 5 cut(s) 2403, 2494, 2717, 3703, 3836
Tsp45I GTSAC 5 cut(s) 2403, 2494, 2717, 3703, 3836
TspGWI ACGGA 3 cut(s) 21, 928, 4528
TspMI CCCGGG 1 cut(s) 4415
Tth111I GACNNNGTC 2 cut(s) 305, 323
Van91I CCANNNNNTGG 2 cut(s) 1520, 4457
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 6 cut(s) 301, 2413, 2438, 4202, 4573, 4712
VspI ATTAAT 3 cut(s) 1517, 3033, 4496
XagI CCTNNNNNAGG 1 cut(s) 1305
XbaI TCTAGA 1 cut(s) 4298
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XmaI CCCGGG 1 cut(s) 4415
XmiI GTMKAC 4 cut(s) 273, 1716, 1945, 4935
XmnI GAANNNNTTC 4 cut(s) 487, 490, 828, 2117
ZraI GACGTC 1 cut(s) 11
Zsp2I ATGCAT 4 cut(s) 457, 792, 2674, 3325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.