Rroxscaffold_7G00187930

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000582
Physical Location & Seq
Forward (+)
27347843 .. 27370600
22758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00187930.1

Sequence Viewer

Length: 6705 bp
ATGGCAATGTTTTCGACATACCCTTTGGTCGGATTACTCAATGTTGCTGTCTCATCTATCAAATGTGGAATGTGGGATAATATGTTTGATACGTCCCAAACCAGTAGTCCACTTGAGTTGGATAACACTGTAACTGATAATTATCCTGAACATGTCGAACAAAGTATAAAGGATGCACCACCAGTCAGCACCGAACATGGACAAACCTCTTGCTGCCTGAAGCCAAATCATCAGGATCGTCCACGTCGGTGGAGGAAGATCCAAAGGAGTCGTAAGAAGTTGGAGCCTGAAAACGGACACATCTGCTACCTTTGTCATCAAGAGGGACACGTCAAGAGAAGTTGCCCTCTGCGGCAGCAAACTAAAAAATCATCTTATGAACCACAGAGTCCTCAACAATTGGCTCAGTATTCTATTTCCGGAGAAGAGGTGGAGGAGAAAGTCAGTGCTGTGGTGGACGGTGTTTCAGTTAAAGAGATAATCAGGAAAGTTGCTACATATTTTGAGACTGATCACGGAATGTCTAGCAACGGTGAATCACTTCCGGAGAAGATATTTATTTACTTCAGAAAGCTTTGCAATTGTGAGCTCTGGTTGACTGAAGAATTTTCTGTCAAGGAATTTAGATCCCTTGGTTATGGGGAATTTATGATGTTTTTAGAAAAGTATGCCCGTCTTCTGCCACCGGAGATGTGCAAGTTCTTGAAAGATGACAGCAGTGGAAAGTTTCCTTTGGAGGTTTGCATGCTTCCGCATTATTTAGTTGTTTTGGTATCTCAAGCTTTAAACAATTTATGGGAGGACCAAAAGATTACCAAACTGAACATTTCTTCTCTGCTTGGGAAGCAGTTCCCGGCCCTTAGTTTCCATACCATTGAAAATGGTTCAGTAGAAGACTTTAAAAGTATAGTAGGAAAGCATAAAGACACTGCAATTTCCAAATGTGTACTATTTTCAATGACAGTATGCGGAACTAGTTACACTCTAGAATCATTGGAAAACTGTAGTAAGAATGCTGTATTGAAAAGCAGTTTGGTGAGTGTTAACAGTGGCCAGAAAATAAGCAATCAATCTGTTGCATCCAAGGAGGCAATTGCAGTCTTACTTAGGGCACCCATGATGTCAGATTTGAATTTATGGTCACATTGGGACCTCTTATTTGCTCCCTCTCTTGGCCCTCTTGTACCGTGGTTATTGAATGAAGTCAACACAGATGAATTACTCTGTTTGGTAACCAAAGATGGCAAAGTTATTCGGTTAGATCATTCAGCTACTGTAGATTCATTCTCGAAAGCTGCTCTTCAAGGATCCTCTTTTCAAACAGCAGTGAAAATGTTATCTCTCTTTTCACTGGTTGGGGGAGAAAAACATGTCCCCGTGTCCCTTTTGAAATTCCATGTACAGCGTGCATTTGAAGTTATTTTCAAGACCTATCTAGATGATATGGAAGTATATGACAAGAAGAGTTCTCTTAATCAAGGAACAGTTTTGTGTGGACAGAAAATGGTTGTTGAAATTGCTGCTGGTAAATGCAGTAACTTACACAGAGCTGTAACGCAGACGAAAATTGCTGAGTCTGTTATATCACATTTTTTTCTTGATTGCCTGGGGTATCTACCTGCAGAGTTTCGCAGTTTTGCTGCTGATGTGTTACTTTCTGGGATGCGGTCTGTTTTTAAGCATGCGGCTTCAGCCATTTTGGATGAATGCATCCAAATAGAGCAGCGTCTCATACTTCATGAAGTTGGCTTATCTCTTGGTATAGTGGAGTGGATTAGTGATTACCATGTATTTAGTTCCTTGGATTCTACTGATTCGTTCATGTCTGAGGATTCTGGTCTCAATGCTGATAGAGATCAAATGTGGTCAGACTCAAAATATATGCAAAATGTGTCAAAAAAGTTGGCTGCTTCTGAACAGAGTATGGCTGCATCTGTTAGGGCAGATGAGCATAAAGGAGGATGTGCTGATGTTAGCCGGATGGTTGATGGTGCAGGAGTTTATGATGCTGGGATTGGCATTGGCATTGGCATTGGTTACAGACAACATCCTACTAATATTAATGAACATGAAGATGCTTTCCAGGTTATTGAGTCAATCAGGAGAGATGAATTCGGTTTGGATTCAAGCCCATTGACCTTTGAAAGTATCGTCTTAAAGAAACAGAATGCTCGCTTAGGGAGAGCACTGCACTGTCTTTCACAGGAATTATATTCTCAAGATTCTCATTTTCTTCTCGAGCTGGTTCAAAATGCTGATGATAATACTTATCCAACAAATATAGAACCAACTCTAGCATTCATTCTTCAAGAATCAGGTATTGTTGTTTTGAATAATGAGCAAGGCTTCTCTGCCCAGAACATTAGAGCACTTTGTGATGTTGGAAATTCAACCAAGAAAGGATCCAGTGCCGGATATATAGGGCAAAAGGGCATCGGTTTCAAATCAGTATTTCGTGTCACAGATGCTCCAGAAATTCATTCTAATGGATTTCATATCAAGTTTGATACAAGTGAGGGTCAGATTGGTTTAATTCTGCCCACAGTTGTACCTCCTTGCAATCTTGAGTTGTTTAGACAGCTGACATGTAGTGACATTGATAAACCAGATTGTTACTGCTGGAAAACTTGTATTGTTCTTCCTTTCAGATCGAAAGTATCAGATGGAACTGTCTTGAACCACATAATGAATATGTTTTCAGATCTTCATCCATCTTTACTACTCTTTCTTCACCGCCTCAAGTGTATCCGGTTTAGAAACCTAATCGATGATTCACTTACTGTCATGAGAAAAGAAATTGTAGGAGATGGTATTGTCAAGGTTTCACATGGGAATACGAAGATGACATGGTTTGTAGTATCTCAGAAATTGCACTCAGATAATCTTCGTAGTGATGTGCAAACAACAGAAATCTCAATTGCTTTTACATTGAAGGAATCGGATAAGGGCATTTATCGTCCAGATATAGGCCAACAGCCTGTTTTTGCATTTCTTCCTCTTAGAACATACGGACTGAAATTTATTGTTCAGGGTGATTTTGTTCTTCCTTCATCCAGAGAGGAAGTGGATGGAGATAGTCCCTGGAACCAATGGCTATTGTCAGAATTTCCTGGTTTATTTGTCGATGCAGAGAAATCATTTTGTTCTCTTCCATGTTTCAAAGAGAATCCTGGGACTGCTGTGGCAGCTTACATGTCCTTTGTTCCACTTGTTGGGGAAGTGCATGGTTTCTTTTCTTCTCTTCCTCGATTAATTATTTCTAAATTACGGATGTCAAATTGCTTACTTCAGGAAGGAGAAAAGAAAGAATGGGTTCCTCCTTGCAAGGTCCTAAGAAATTGGAGTAAACAGGCTCGTTCACTTCTCCCTGATGGTCTACTGCATGAGCATCTTGGCCTTGGATTCTTGGATAAAGATATAGTTCTGCCTGATCCACTTGCAAGAGTTCTAGGTATTGCAGAGTATGGGCCCACAGTTCTGCTTCAGGTAATGGCTTCTTTATGTGGTAAGCAGGATGGCCTCAAATCAATGGGAATGGTCGTTGAAGGCCCAATTTGGCTACAGTTTGATGCCGTTGGGACTGGTTTTGGGGATCATCATGGACTTGAATCTTTTCCAAAGTTATATGCTAAACTTCGGATTGTTAGTCCGGAGCTCTATGCATCGTCTGCTGATATGCCATCCATGGATGTGACGCCGATTGACAAAGTCATTAGTATGCTCCATAGAATTGGTGTCCAACGGTTGTCCGCACACGAGATTCTCAAGGCTCACATCTTGCCAGCTATTTCTGATGATAGAATTACAGTTAGGGATGCAGATTTGATGACTGATTATATCTGCTTTGTAATGGTTCACTTACAGTCGAGCTGCTCTGATTGTCATGCTGAAAGGGAATACATAATATCAGAACTACAAAATAAAGCTTATATTCTAACTAATAATGGCTTTAAACGACCTGCTGAGGCATCAATTCATTTCAGTAAAGAATTTGGAAATCCAGTGGACATAAGTAGGTTGATTGATAGGGTAAATCTGACATGGGATGAGGTTGATATCTCCTATTTGAAGCATCCAGTTGCTAAATCATTTCCAAATGGACTGATGAAGTGGAGAGATTTTTTCCAGAAAATTGGCATTGTGGATTTTGTGAAGGTAGTTCAAGTTGAGAAGGGTTTCACTGAGTTGTCTGAGGCTCTATTTAATAATCTAACTTGGGACCAGCACACTATTTCCCATGGGTTGAATGCCACAGATTGGGAATCACCTGAGTTAGTCCATTTATTATCCCTATTGTCCAGAGATGGCAACAGAAAAGGTTGTGAGTATCTCTTAGAGGTTCTCGATAAATTATGGGATGATTGCTATAGTGACAGAGCTACAGGGTACTGCTCTTCTAAGTCTGTTGCGGATAGAAAGCCCTTCAAATCTTCATTTATGAGCACCATCTGCGATGTGCAATGGGTAGTATCAGGAATGGATGATAAACTGCACTATCCCAAGGATCTATATCACGACAGTGTTGCAGTGCGGTCAATTCTTGGTGGATCTGCCCCATTTTTTGTTCCTAAGGCTACCAGTGAAAAATTTGTATCTGATATTGGTTTTAAGACAAGAGTCTCTCTTCACGATGCTCTGGAGATGTTGAAACTATGGAGATGTGAGAATCCGTTTAGGGCCAGCCTAGCACAAATGTCCAAATTTTACTCATTAATCTGGAATGAAATGGCTTCTTCAAAGAAGACAACTGCAGAGGAATTCTGTTTGGAACCTTTTGTATTTGTTCCATATGAATCTAGCTTCCGGCATGAGGATGTGGTGTCTGGTACATTTCTGTCCCTTGAAGAAGTATACTGGGATGATTCTACTTTCTTTGTGGACCAAATCAAGGAGATCCATCATCAGTGCAACTCAACAGTTGGTAATCATGGCCCCATAAACAGGATTCTGAGTAATTTCTACCCGGCCCTTCATGACTTCTTTGTTGATATATGTGGAGTACATGAGATCCCTCCTCTTCGTAGCTACCTTCAGATTTTGCTACAGTTTTCAAATGCTGTTTTGCCTTCACAAGCAGCTAATGCTGTTTTTCAAGTTTTTCAGAAGTGGGCTGATGGGCTCCAATCAGGAATGAGTGCTGAGGATATTGTATACTTGAAAGATTCTCTTACAAGGATGGAATGTACTGTGCTTCCTACTGTACAGGATAAGTGGGTATCTCTACATCCTTCCTATGGTCTCCTGTGCTGGTGTGATGATAAGAAGTTGAAGAAGCAGTTTATGCATATGGATGGACTTGATTTTCTTTACTCCGGTGGACTAAGTAATGATGATGAAGAGATCCTAAGTACAAAAATGTCCGTCCTCATGCGAACCTTAGGGATTCCTGCACTTTCAGAGGTTGTGACTCGTGAGGCGATGTTTAATGGTCCGACAGATTCTAGCTTTAAAGCTGCATTGCTGGATTGGGCTCTGCCATATGCGCAGCGCTACTTACATAGTTTACATCCTGATAAATATTCTCAACTCAAGCAGTCTGGATTCGATATACTAAATCGTTTGCGAGTAGTAGAAGTTCAAAAGCTGTCCTCCCGCAATGTTATAAAGATTGCTGGAAGTGAATCCAAGAAGCAAATCGACTGTAGTTGTGTTCTGCAGGATCACGATCTGTATACAACCCAGGAATCAGATTCTCACGCATTATTTATGGAGCTTTCTCGTTTGTTTTTTGATGGAAAACCAGAATTACACTTGGCAAATTTCCTTCATATGATCACAACAATGGCTGAATCAGGTTCAACTGAGGAGCAGATAGACTTTTTTATCTTCAATAGCCAAAAGGTGCCAAAGCTTCCTGATGGAGAATCTGTTTGGTCCCTGTCACATGTGCATAATGATAGGTTACCCCAGCCAAGTGACTGGCGCTTTGTTGGTTGGAAAGCCCCACTAAGCTTTGGTTATGCAAATCTTTTCAAGGCTCAGGCACAATTTGCAGAGCTTACCAGTGTCTCACAAATTGAAATGGATAATGATTCTGAAACCCATGTCAGACAAACAGTTGAATCAACTCCCATTTCAGTCGACATTAAATGGACAATTGGTGAGGGTACAGCAACAACATCTGCACCTTTAGTGCTGCCAAACTCCAATGATTTGCAAGAACACTTTGATGATGAGACTGATATGGATACTGATTTCAATCCTAATAATCTAGATTTTGTTGTTGACCCTCGCGTTTTGGGTTCATCTGATTGTAGCAAGGTCGACCATCCTAGATATGGCTCTAGTGGAAGAGATGCAATGCGTGGAAGAGATGCAATGCTTACGGGGAGGTTAGGTGAGGTTGTTGCTTTTAAATACTTAATTGCAAAAGCTGGCAAGTCAGTTGTGAGGTGGGTTAATGAAAGAATTGAAACTGGGCTACCCTATGACATAGTTGTAGGTGAGAAGGAAGATAGTCTAGAGTTCATTGAGGTTAAAGCCACCCAACAACAAAAAAAGGATTGGTTCCACATATCAATGAGGGAGTGGCAATTTGCAGCGGAAAAAGGCGAAGCCTTCAGCATTTTGCACGTCCTCTTATTGGGTAATAATGCTGCAAGAGTCTCAGTTTACAAGAATCCTGTAAAATTGTGTCATCTGGGCAAGCTACAGTTGCATCTTTCGATGCCTAGGCAGGAGAAGGAATTATTTCTTGTGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

2234

Amino Acids

251.22

Weight (kDa)

5.71

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 742 - 836 7.3e-09 Sacsin domain
NOV_C PF13020 2113 - 2199 1.4e-19 Protein NO VEIN, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5603
Acc16I TGCGCA 1 cut(s) 5484
Acc36I ACCTGC 2 cut(s) 1627, 3967
AccB1I GGYRCC 2 cut(s) 1111, 5842
AccB7I CCANNNNNTGG 2 cut(s) 3212, 4257
AccI GTMKAC 6 cut(s) 3376, 4851, 5151, 5672, 6081, 6264
AccII CGCG 1 cut(s) 6234
AccIII TCCGGA 3 cut(s) 419, 544, 3649
AcoI YGGCCR 1 cut(s) 1051
AcuI CTGAAG 8 cut(s) 239, 550, 621, 1674, 3272, 3467, 5015, 6544
AcyI GRCGYC 1 cut(s) 3695
AdeI CACNNNGTG 1 cut(s) 2375
AfeI AGCGCT 1 cut(s) 5489
AflIII ACRYGT 6 cut(s) 151, 328, 1369, 2585, 3192, 5884
AhlI ACTAGT 1 cut(s) 974
AjiI CACGTC 3 cut(s) 245, 331, 6574
AjnI CCWGG 6 cut(s) 1605, 2082, 3080, 3109, 3169, 5679
AjuI GAANNNNNNNTTGG 2 cut(s) 1016, 1048
AleI CACNNNNGTG 2 cut(s) 247, 4518
Alw21I GWGCWC 5 cut(s) 591, 2188, 2371, 3657, 4445
Alw26I GTCTC 9 cut(s) 55, 500, 1733, 1844, 4624, 5243, 6013, 6170, 6610
AlwNI CAGNNNCTG 1 cut(s) 1274
Ama87I CYCGRG 1 cut(s) 2237
Aor13HI TCCGGA 3 cut(s) 419, 544, 3649
Aor51HI AGCGCT 1 cut(s) 5489
ApaI GGGCCC 1 cut(s) 3471
ArsI GACNNNNNNTTYG 8 cut(s) 7, 39, 91, 123, 1142, 1174, 5909, 5941
AseI ATTAAT 3 cut(s) 2061, 3251, 4712
Asp700I GAANNNNTTC 6 cut(s) 540, 848, 3312, 3612, 4175, 6690
AspA2I CCTAGG 1 cut(s) 6671
AspLEI GCGC 3 cut(s) 5485, 5490, 5925
AsuC2I CCSGG 2 cut(s) 854, 4964
AsuHPI GGTGA 8 cut(s) 545, 1048, 2723, 3044, 4257, 6113, 6350, 6455
AvaI CYCGRG 1 cut(s) 2237
AvaII GGWCC 7 cut(s) 802, 1150, 3328, 4219, 4879, 5429, 5874
AvrII CCTAGG 1 cut(s) 6671
AxyI CCTNAGG 2 cut(s) 4569, 5377
BaeGI GKGCMC 2 cut(s) 1114, 3471
BaeI ACNNNNGTAYC 2 cut(s) 2532, 2565
BalI TGGCCA 1 cut(s) 1053
BamHI GGATCC 2 cut(s) 1307, 2402
BanI GGYRCC 2 cut(s) 1111, 5842
BanII GRGCYC 5 cut(s) 591, 3471, 3657, 5121, 5473
BauI CACGAG 2 cut(s) 3755, 5409
BbsI GAAGAC 3 cut(s) 668, 900, 4748
Bbv12I GWGCWC 5 cut(s) 591, 2188, 2371, 3657, 4445
BbvCI CCTCAGC 2 cut(s) 3963, 5139
BceAI ACGGC 1 cut(s) 3557
BcgI CGANNNNNNTGC 3 cut(s) 28, 4505, 4539
BciT130I CCWGG 6 cut(s) 1607, 2084, 3082, 3111, 3171, 5681
BciVI GTATCC 2 cut(s) 2756, 6181
BclI TGATCA 2 cut(s) 511, 5772
BcnI CCSGG 2 cut(s) 854, 4964
BcoDI GTCTC 9 cut(s) 55, 500, 1733, 1844, 4624, 5243, 6013, 6170, 6610
BcuI ACTAGT 1 cut(s) 974
BfoI RGCGCY 2 cut(s) 5491, 5926
BfuAI ACCTGC 2 cut(s) 1627, 3967
BfuI GTATCC 2 cut(s) 2756, 6181
BglII AGATCT 1 cut(s) 2701
BlnI CCTAGG 1 cut(s) 6671
Bme1390I CCNGG 8 cut(s) 854, 1607, 2084, 3082, 3111, 3171, 4964, 5681
Bme18I GGWCC 7 cut(s) 802, 1150, 3328, 4219, 4879, 5429, 5874
BmeT110I CYCGRG 1 cut(s) 2237
BmgBI CACGTC 3 cut(s) 245, 331, 6574
BmrFI CCNGG 8 cut(s) 854, 1607, 2084, 3082, 3111, 3171, 4964, 5681
BmrI ACTGGG 2 cut(s) 4864, 6426
BmuI ACTGGG 2 cut(s) 4864, 6426
BoxI GACNNNNGTC 1 cut(s) 4616
BpiI GAAGAC 3 cut(s) 668, 900, 4748
BpmI CTGGAG 2 cut(s) 2454, 4658
Bpu10I CCTNAGC 4 cut(s) 2176, 3963, 5139, 5979
BpuEI CTTGAG 7 cut(s) 134, 762, 2202, 2585, 2723, 3749, 5513
BpuMI CCSGG 2 cut(s) 854, 4964
Bsa29I ATCGAT 1 cut(s) 2766
BsaBI GATNNNNATC 6 cut(s) 141, 1854, 2706, 4509, 5664, 6198
BsaHI GRCGYC 1 cut(s) 3695
BsaI GGTCTC 2 cut(s) 1844, 5243
BsaWI WCCGGW 6 cut(s) 419, 544, 685, 2748, 3649, 5312
BsaXI ACNNNNNCTCC 8 cut(s) 414, 444, 2452, 2482, 5853, 5883, 6322, 6352
Bse21I CCTNAGG 2 cut(s) 4569, 5377
Bse3DI GCAATG 6 cut(s) 12, 4466, 5456, 5602, 6306, 6324
Bse8I GATNNNNATC 6 cut(s) 141, 1854, 2706, 4509, 5664, 6198
BseAI TCCGGA 3 cut(s) 419, 544, 3649
BseBI CCWGG 6 cut(s) 1607, 2084, 3082, 3111, 3171, 5681
BseCI ATCGAT 1 cut(s) 2766
BseJI GATNNNNATC 6 cut(s) 141, 1854, 2706, 4509, 5664, 6198
BseMI GCAATG 6 cut(s) 12, 4466, 5456, 5602, 6306, 6324
BseRI GAGGAG 3 cut(s) 449, 5004, 5819
BseSI GKGCMC 2 cut(s) 1114, 3471
BseYI CCCAGC 2 cut(s) 2009, 5907
BsgI GTGCAG 5 cut(s) 2013, 2174, 4475, 5373, 6108
Bsh1236I CGCG 1 cut(s) 6234
BshNI GGYRCC 2 cut(s) 1111, 5842
BshVI ATCGAT 1 cut(s) 2766
BsiHKAI GWGCWC 5 cut(s) 591, 2188, 2371, 3657, 4445
BsiHKCI CYCGRG 1 cut(s) 2237
BsmAI GTCTC 9 cut(s) 55, 500, 1733, 1844, 4624, 5243, 6013, 6170, 6610
BsmBI CGTCTC 1 cut(s) 1733
BsmI GAATGC 5 cut(s) 1018, 1712, 2173, 2297, 4252
Bso31I GGTCTC 2 cut(s) 1844, 5243
BsoBI CYCGRG 1 cut(s) 2237
Bsp120I GGGCCC 1 cut(s) 3467
Bsp1286I GDGCHC 9 cut(s) 591, 1114, 2188, 2371, 3471, 3657, 4445, 5121, 5473
Bsp13I TCCGGA 3 cut(s) 419, 544, 3649
Bsp1407I TGTACA 2 cut(s) 1399, 5200
Bsp19I CCATGG 2 cut(s) 3684, 4237
BspDI ATCGAT 1 cut(s) 2766
BspEI TCCGGA 3 cut(s) 419, 544, 3649
BspFNI CGCG 1 cut(s) 6234
BspHI TCATGA 3 cut(s) 1738, 2784, 4972
BspMAI CTGCAG 3 cut(s) 1624, 4753, 5658
BspMI ACCTGC 2 cut(s) 1627, 3967
BspQI GCTCTTC 2 cut(s) 1305, 4399
BspT107I GGYRCC 2 cut(s) 1111, 5842
BspTNI GGTCTC 2 cut(s) 1844, 5243
BsrDI GCAATG 6 cut(s) 12, 4466, 5456, 5602, 6306, 6324
BsrGI TGTACA 2 cut(s) 1399, 5200
BssNAI GTATAC 3 cut(s) 4852, 5152, 5673
BssNI GRCGYC 1 cut(s) 3695
BssSI CACGAG 2 cut(s) 3755, 5409
BssT1I CCWWGG 8 cut(s) 631, 1083, 1800, 3397, 3684, 4237, 4500, 6671
Bst1107I GTATAC 3 cut(s) 4852, 5152, 5673
Bst2BI CACGAG 2 cut(s) 3755, 5409
Bst2UI CCWGG 6 cut(s) 1607, 2084, 3082, 3111, 3171, 5681
BstACI GRCGYC 1 cut(s) 3695
BstAPI GCANNNNNTGC 5 cut(s) 3668, 4449, 5081, 5281, 5990
BstAUI TGTACA 2 cut(s) 1399, 5200
BstC8I GCNNGC 8 cut(s) 746, 1407, 1683, 2173, 3783, 4681, 6376, 6647
BstDSI CCRYGG 3 cut(s) 1187, 3684, 4237
BstEII GGTNACC 2 cut(s) 1231, 5901
BstFNI CGCG 1 cut(s) 6234
BstH2I RGCGCY 2 cut(s) 5491, 5926
BstHHI GCGC 3 cut(s) 5485, 5490, 5925
BstMAI GTCTC 9 cut(s) 55, 500, 1733, 1844, 4624, 5243, 6013, 6170, 6610
BstNI CCWGG 6 cut(s) 1607, 2084, 3082, 3111, 3171, 5681
BstNSI RCATGY 7 cut(s) 155, 748, 1373, 1685, 2589, 3196, 5888
BstPAI GACNNNNGTC 1 cut(s) 4616
BstPI GGTNACC 2 cut(s) 1231, 5901
BstSCI CCNGG 8 cut(s) 852, 1605, 2082, 3080, 3109, 3169, 4962, 5679
BstSLI GKGCMC 2 cut(s) 1114, 3471
BstUI CGCG 1 cut(s) 6234
BstV2I GAAGAC 3 cut(s) 668, 900, 4748
BstXI CCANNNNNNTGG 4 cut(s) 249, 3731, 4133, 5919
BstZ17I GTATAC 3 cut(s) 4852, 5152, 5673
Bsu15I ATCGAT 1 cut(s) 2766
Bsu36I CCTNAGG 2 cut(s) 4569, 5377
BsuI GTATCC 2 cut(s) 2756, 6181
BsuTUI ATCGAT 1 cut(s) 2766
BtgI CCRYGG 3 cut(s) 1187, 3684, 4237
BtgZI GCGATG 2 cut(s) 4467, 5432
BtrI CACGTC 3 cut(s) 245, 331, 6574
BtsI GCAGTG 5 cut(s) 724, 927, 1332, 2186, 4533
BveI ACCTGC 2 cut(s) 1627, 3967
Cac8I GCNNGC 8 cut(s) 746, 1407, 1683, 2173, 3783, 4681, 6376, 6647
CaiI CAGNNNCTG 1 cut(s) 1274
CciI TCATGA 3 cut(s) 1738, 2784, 4972
CfoI GCGC 3 cut(s) 5485, 5490, 5925
ClaI ATCGAT 1 cut(s) 2766
CseI GACGC 2 cut(s) 1715, 3703
CspCI CAANNNNNGTGG 2 cut(s) 99, 134
DraI TTTAAA 5 cut(s) 786, 901, 3952, 5449, 6355
DraIII CACNNNGTG 1 cut(s) 2375
EaeI YGGCCR 1 cut(s) 1051
Ecl136II GAGCTC 2 cut(s) 589, 3655
Eco130I CCWWGG 8 cut(s) 631, 1083, 1800, 3397, 3684, 4237, 4500, 6671
Eco24I GRGCYC 5 cut(s) 591, 3471, 3657, 5121, 5473
Eco31I GGTCTC 2 cut(s) 1844, 5243
Eco32I GATATC 1 cut(s) 4057
Eco47I GGWCC 7 cut(s) 802, 1150, 3328, 4219, 4879, 5429, 5874
Eco47III AGCGCT 1 cut(s) 5489
Eco53kI GAGCTC 2 cut(s) 589, 3655
Eco57I CTGAAG 8 cut(s) 239, 550, 621, 1674, 3272, 3467, 5015, 6544
Eco81I CCTNAGG 2 cut(s) 4569, 5377
Eco88I CYCGRG 1 cut(s) 2237
Eco91I GGTNACC 2 cut(s) 1231, 5901
EcoICRI GAGCTC 2 cut(s) 589, 3655
EcoO109I RGGNCCY 2 cut(s) 1150, 3328
EcoO65I GGTNACC 2 cut(s) 1231, 5901
EcoRI GAATTC 2 cut(s) 2111, 4757
EcoRII CCWGG 6 cut(s) 1605, 2082, 3080, 3109, 3169, 5679
EcoRV GATATC 1 cut(s) 4057
EcoT14I CCWWGG 8 cut(s) 631, 1083, 1800, 3397, 3684, 4237, 4500, 6671
EcoT22I ATGCAT 3 cut(s) 1712, 3664, 5286
EcoT38I GRGCYC 5 cut(s) 591, 3471, 3657, 5121, 5473
ErhI CCWWGG 8 cut(s) 631, 1083, 1800, 3397, 3684, 4237, 4500, 6671
Esp3I CGTCTC 1 cut(s) 1733
FalI AAGNNNNNCTT 4 cut(s) 1284, 1316, 5151, 5183
FauI CCCGC 1 cut(s) 5600
FauNDI CATATG 4 cut(s) 4789, 5286, 5479, 5769
FbaI TGATCA 2 cut(s) 511, 5772
FblI GTMKAC 6 cut(s) 3376, 4851, 5151, 5672, 6081, 6264
FriOI GRGCYC 5 cut(s) 591, 3471, 3657, 5121, 5473
FspI TGCGCA 1 cut(s) 5484
GlaI GCGC 3 cut(s) 5484, 5489, 5924
GsaI CCCAGC 2 cut(s) 2013, 5911
GsuI CTGGAG 2 cut(s) 2454, 4658
HaeII RGCGCY 2 cut(s) 5491, 5926
HgaI GACGC 2 cut(s) 1715, 3703
HhaI GCGC 3 cut(s) 5485, 5490, 5925
Hin1I GRCGYC 1 cut(s) 3695
Hin6I GCGC 3 cut(s) 5483, 5488, 5923
HinP1I GCGC 3 cut(s) 5483, 5488, 5923
HincII GTYRAC 6 cut(s) 597, 1045, 1207, 6082, 6226, 6265
HindII GTYRAC 6 cut(s) 597, 1045, 1207, 6082, 6226, 6265
HindIII AAGCTT 5 cut(s) 572, 780, 3924, 5849, 5950
HpaI GTTAAC 1 cut(s) 1045
HphI GGTGA 8 cut(s) 545, 1048, 2723, 3044, 4257, 6113, 6350, 6455
Hpy99I CGWCG 1 cut(s) 249
HpyCH4IV ACGT 4 cut(s) 92, 244, 330, 6573
HpySE526I ACGT 4 cut(s) 92, 244, 330, 6573
Hsp92I GRCGYC 1 cut(s) 3695
HspAI GCGC 3 cut(s) 5483, 5488, 5923
Kpn2I TCCGGA 3 cut(s) 419, 544, 3649
Ksp22I TGATCA 2 cut(s) 511, 5772
KspAI GTTAAC 1 cut(s) 1045
LguI GCTCTTC 2 cut(s) 1305, 4399
LmnI GCTCC 8 cut(s) 283, 1168, 2473, 3652, 3726, 5124, 5710, 5806
MaeII ACGT 4 cut(s) 92, 244, 330, 6573
MfeI CAATTG 5 cut(s) 398, 580, 1092, 2916, 6096
MhlI GDGCHC 9 cut(s) 591, 1114, 2188, 2371, 3471, 3657, 4445, 5121, 5473
MlsI TGGCCA 1 cut(s) 1053
MluNI TGGCCA 1 cut(s) 1053
MlyI GAGTC 8 cut(s) 277, 397, 1583, 1865, 2102, 4626, 5401, 6612
MmeI TCCRAC 8 cut(s) 10, 99, 261, 2297, 2362, 3763, 5456, 5915
Mox20I TGGCCA 1 cut(s) 1053
Mph1103I ATGCAT 3 cut(s) 1712, 3664, 5286
MroI TCCGGA 3 cut(s) 419, 544, 3649
MroXI GAANNNNTTC 6 cut(s) 540, 848, 3312, 3612, 4175, 6690
MscI TGGCCA 1 cut(s) 1053
Msp20I TGGCCA 1 cut(s) 1053
MspA1I CMGCKG 2 cut(s) 2581, 6542
MspR9I CCNGG 8 cut(s) 854, 1607, 2084, 3082, 3111, 3171, 4964, 5681
MteI GCGCNGCGC 1 cut(s) 5486
MunI CAATTG 5 cut(s) 398, 580, 1092, 2916, 6096
Mva1269I GAATGC 5 cut(s) 1018, 1712, 2173, 2297, 4252
MvaI CCWGG 6 cut(s) 1607, 2084, 3082, 3111, 3171, 5681
MvnI CGCG 1 cut(s) 6234
NciI CCSGG 2 cut(s) 854, 4964
NcoI CCATGG 2 cut(s) 3684, 4237
NdeI CATATG 4 cut(s) 4789, 5286, 5479, 5769
NmuCI GTSAC 8 cut(s) 1140, 2458, 2591, 3691, 4370, 5404, 5880, 5915
NsbI TGCGCA 1 cut(s) 5484
NsiI ATGCAT 3 cut(s) 1712, 3664, 5286
NspI RCATGY 7 cut(s) 155, 748, 1373, 1685, 2589, 3196, 5888
OliI CACNNNNGTG 2 cut(s) 247, 4518
PaeI GCATGC 2 cut(s) 748, 1685
PaeR7I CTCGAG 1 cut(s) 2237
PagI TCATGA 3 cut(s) 1738, 2784, 4972
PciI ACATGT 5 cut(s) 151, 1369, 2585, 3192, 5884
PciSI GCTCTTC 2 cut(s) 1305, 4399
PctI GAATGC 5 cut(s) 1018, 1712, 2173, 2297, 4252
PdmI GAANNNNTTC 6 cut(s) 540, 848, 3312, 3612, 4175, 6690
PflFI GACNNNGTC 1 cut(s) 3707
PflMI CCANNNNNTGG 2 cut(s) 3212, 4257
PleI GAGTC 8 cut(s) 276, 396, 1582, 1865, 2101, 4625, 5401, 6611
PpsI GAGTC 8 cut(s) 276, 396, 1582, 1865, 2101, 4625, 5401, 6611
PpuMI RGGWCCY 2 cut(s) 1150, 3328
PscI ACATGT 5 cut(s) 151, 1369, 2585, 3192, 5884
PshAI GACNNNNGTC 1 cut(s) 4616
PshBI ATTAAT 3 cut(s) 2061, 3251, 4712
PsiI TTATAA 1 cut(s) 5603
Psp124BI GAGCTC 2 cut(s) 591, 3657
Psp5II RGGWCCY 2 cut(s) 1150, 3328
Psp6I CCWGG 6 cut(s) 1605, 2082, 3080, 3109, 3169, 5679
PspEI GGTNACC 2 cut(s) 1231, 5901
PspFI CCCAGC 2 cut(s) 2009, 5907
PspGI CCWGG 6 cut(s) 1605, 2082, 3080, 3109, 3169, 5679
PspOMI GGGCCC 1 cut(s) 3467
PspPPI RGGWCCY 2 cut(s) 1150, 3328
PsrI GAACNNNNNNTAC 2 cut(s) 5559, 5591
PstI CTGCAG 3 cut(s) 1624, 4753, 5658
PstNI CAGNNNCTG 1 cut(s) 1274
PsyI GACNNNGTC 1 cut(s) 3707
PvuII CAGCTG 1 cut(s) 2581
SacI GAGCTC 2 cut(s) 591, 3657
SalI GTCGAC 2 cut(s) 6080, 6263
SapI GCTCTTC 2 cut(s) 1305, 4399
SchI GAGTC 8 cut(s) 277, 397, 1583, 1865, 2102, 4626, 5401, 6612
ScrFI CCNGG 8 cut(s) 854, 1607, 2084, 3082, 3111, 3171, 4964, 5681
SduI GDGCHC 9 cut(s) 591, 1114, 2188, 2371, 3471, 3657, 4445, 5121, 5473
Sfr274I CTCGAG 1 cut(s) 2237
SinI GGWCC 7 cut(s) 802, 1150, 3328, 4219, 4879, 5429, 5874
SlaI CTCGAG 1 cut(s) 2237
SmlI CTYRAG 8 cut(s) 113, 777, 2217, 2237, 2564, 2738, 3764, 5528
SmoI CTYRAG 8 cut(s) 113, 777, 2217, 2237, 2564, 2738, 3764, 5528
SpeI ACTAGT 1 cut(s) 974
SphI GCATGC 2 cut(s) 748, 1685
SspI AATATT 2 cut(s) 2059, 5519
SstI GAGCTC 2 cut(s) 591, 3657
StyD4I CCNGG 8 cut(s) 852, 1605, 2082, 3080, 3109, 3169, 4962, 5679
StyI CCWWGG 8 cut(s) 631, 1083, 1800, 3397, 3684, 4237, 4500, 6671
TaiI ACGT 4 cut(s) 95, 247, 333, 6576
TatI WGTACW 6 cut(s) 946, 1399, 4999, 5183, 5200, 5348
TauI GCSGC 2 cut(s) 355, 1688
TseFI GTSAC 8 cut(s) 1140, 2458, 2591, 3691, 4370, 5404, 5880, 5915
Tsp45I GTSAC 8 cut(s) 1140, 2458, 2591, 3691, 4370, 5404, 5880, 5915
TspGWI ACGGA 6 cut(s) 309, 531, 3024, 3283, 4659, 5350
Tth111I GACNNNGTC 1 cut(s) 3707
Van91I CCANNNNNTGG 2 cut(s) 3212, 4257
VpaK11BI GGWCC 7 cut(s) 802, 1150, 3328, 4219, 4879, 5429, 5874
VspI ATTAAT 3 cut(s) 2061, 3251, 4712
XbaI TCTAGA 4 cut(s) 985, 1435, 6211, 6460
XceI RCATGY 7 cut(s) 155, 748, 1373, 1685, 2589, 3196, 5888
XcmI CCANNNNNNNNNTGG 2 cut(s) 3061, 6275
XhoI CTCGAG 1 cut(s) 2237
XmaJI CCTAGG 1 cut(s) 6671
XmiI GTMKAC 6 cut(s) 3376, 4851, 5151, 5672, 6081, 6264
XmnI GAANNNNTTC 6 cut(s) 540, 848, 3312, 3612, 4175, 6690
Zsp2I ATGCAT 3 cut(s) 1712, 3664, 5286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.