Prupe.1G159000_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000582
Physical Location & Seq
Reverse (-)
12603659 .. 12618027
14369 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G159000.1

Sequence Viewer

Length: 6822 bp
ATGGCAGCCTGCATGGAGCAGGATCAACAGTATTATCCACGTCGTGAGAGAGTCCGTAGTAGGCGGAAGGTCCTCCAAATGAGTCAGAAGAACAACATACACAAAGGGTTATCCGGAGGATCTCCTTCTGAATCAAAAGTCTGCTTTCTGTGTGGTCAAGCCGGACACGTCTGTAAGGACTGCTCTCAACTCCAACAGGCTGTATCATCTTTGGCTCCACAGAATCCCCAGAAATTGGTTCGGTACCCTGCTCAGTCCTACACATCTGTTCAACTACTTCCAGTTGAGTCCCAGCCACAACAGAGCACGGACAAAGCACCAATGCCCCAGAAAAGTAGATGTGGACCTGTAGGTAGGGGTCAAGTCTTCAGAGTCACCGGAGGAGAAGCGATGGAAGAGAGAGTCAATGCTGTGATCAATGACTTCACAGGTGTTTCAGTTGAAGACATAATCAGGAAAGTTGCGACGTTTTTTGAGAGTGATCATGGAATGCCCATCAATGGTAAAACAATTCAGGAGAAGATGTTTATCTTTTTCAGAAAGCTTTGCAATTGTGAGCTTTGGTTGGTGGAAGAATTTTGTGTCAAGGAATTTAGATGCCTTGGTTTTGGGGAATTTCTAATGTTCTTAGAAAAATATTCCTGTCTACTGCCACATGAGCTGTGCAAGTTCTTGACTGGTGACGGAAATGGGAAGTGCCCTTTGGAGGTTTGCATGCCCCAGGATCATTTGGTTGTTTTAGTATCTCAAGCTTTAAATAGTTTATGGGAGGATGAAAAGATTACCAAACTGAATATTCCTTTACTGCTTAGGAAGCAGTTCCCATCGATTGGTTTCCAAACCATCGAAAATGGTTCGGTAGAAGATTTTCTAAGTTTAGTGGGGAAGCATAAAAACACTGCAATTTCCAAACGTGTTCTTTTTTCAATGACATTATGTGGAACTAGTTACGGTATGGAGTCACCAGAACACTGTAAGAATGACACATTTAGGAGCACTTTGGTGAATGCTAACATTGGCCAGACAGCAGAAACCCATGAATCTGTTACATCCAAAGATGCAATTAAAATCCTACTCAGGGGACCAATGATGTCTGATTTAAACTTATGGGCACATTGGGACCTCTTGTTTGCTCCCTATCTTGGTACTCTTGTACCATGGTTATTGAATGAAGTCAACACGGATGAATTGTTGTGTTTGGTGACCAAAGATGGGAAAGTAATTCGGTTAGACCATTCATCTACTGTAGATGCATTCTTGGAAGCTGTACTTCAAGGCTCCTCTTTCCAAACAGCAGTGAAAATGCTCTCTCTGTTTGCACTATTTGGGGGAGAAAAGCATGTGCCTGTGTCCCTTCTGAAACTTCATGTACAGCATGCATTTGAAGTCATTTTGAAGAATTATGTAGACAATATGGAAATACATGGCAATAAGAATTTTCTAAGTCAAGGAGAAGCATTGTGTGGACAGCAAATGATTGGTGAAGGTTCTACTAGTAAATTTTGTAATGAATTAAACAGAGATGTAACCCGGATGAATACTGGTGAACCTGTTTTATCAAGATTTATCATTGATTGTCTTGGCTATCTACCTGCAGAATTTCGTAGTTTTGCTGCTGATGTATTGCTTTCAGGGATGCGATCTATTTTCAAACATGCTGCTTCAGCCATTTTGAGTGAGTGCAACCAGACAGAGCAGCGTCTCATGATTCATGAAGTTGGCTTATCTCTTGGTATAGTGGAGTGGATTCATGATTACAATGAATTTTGTTCCTATGACACTACTGATTTGTTCATGTCTGGGGCTACATGCTTCAATGCTGTTAGATATGAAACCGGGTCAGGCTCAAAGTATATGCAGGATGTGTCTGATACGCTTGCCGCTTCTGAAGGGAGTATTGGTGCATCTGTTGGGGTAGATGAGCAGAAAGGTGAATGTACTGAACCCTGTATAAAGATTAGTGGTGCAGAAGCTTCTGAAGCTAGAATTGGCAGTGGCTCAAAGCATTCTACAGAACTTAATGAACTTGAAGATGCAGTCCTCGTAATTGAGTCAATCAGGAGAGATGAATTTGGTCTAGATTCAAGCCTATCAAATGTTGGAAGTAGCATCCTAAAGAAGCAGCATGCCCGCTTAGGGAGAGCTCTGCATTGCCTTTCAGAGGAATTGTATTCTCAGGATTCTCATTTTCTTCTTGAGCTGGTGCAAAATGCTGATGATAATATTTACCCAACAAATGTGGAACCAACTCTAACATTCATTCTTCAAGAGTCAGGTATTGTTGTTTTAAACAATGAACGGGGCTTTTCTGCCCAGAACATTAGGGCACTCTGTGATGTTGGAAATTCAACCAAGAAAGGATCTAAAGCTGGATATATAGGGCAGAAGGGCATTGGTTTCAAATCAGTATTTCGGGTTACAGATGCTCCAGAAATTCATTCCAATGGGTTTCATATCAAGTTTGATATAAGTGAGGGTCAGATTGGTTTAATTTTGCCCACAGTTGTACCTCCTCGCAGTATTCACTTGTTTAGTAGCCTGGCATCAAGTGACAATGATAAAGCGGATTGGAACTGCTGGAACACTTGCATTGTTCTTCCTTTCAGATCTATTTTATCAGATAGAACTGTCATGAAAAGTGTAATTAATATGTTTTCAGATCTTCATCCTTCTTTACTACTCTTTCTCCACCGCCTCCAGTGTATTAAGTTTAGAAACTTGCTTGATGATACACTTACGGTCATGAGAAAGGATATTGTGGGAGATGGTATCATTAAGATTTCACATGGGAAAGAGAAAATGACATGGTTTGTAGTATCTCAGAAATTGCAAGCAGATTTTATTCGTGGTGATGTACAAACAACAGAAATATCAATAGCATTTACATTGAAGGAATCAGATAATGGCGATTATTGTCCTGATATTGCCCCACAGCCTGTTTTTGCATTTCTTCCTCTTAGAACATATGGGCTGAAGTTTATTCTTCAGGGTGATTTTGTTCTTCCATCGTCTAGAGAAGAAGTCGATGGAGATAGTCCCTGGAACCAATGGCTATTGTCCGAATTTCCTGGTTTGTTTGTTAACGCAGAGAGATCTTTTTGTGCTCTTCCGTGCTTTAAAGAGAATCCCGGGAGAGCTGTGACAGCTTATATGTCCTTTGTTCCGCTTGTCGGGGATGTGCATGGTTTCTTTTCTTCTCTTCCGCGATTGATTATTTCAAAATTACGTGTGTCGAATTGCTTACTCCTGGAAGGAGGAAATAATGAATGGGTTCCTCCGTGCAGGGTCCTAAGAGGTTGGAATAAACAGGCTCGTTTGGTTCTCCCTGATGACTTACTGCAAGAGCATCTTGGACTTGGGTTCTTACATAAAAATATAGTTGTGCCGGATCCACTTGCAAGAGTTCTAGGTATTGTGGACTATGGACCCAAAGTTTTGCTTCAAATTGTGGACTCTTTATGTCATACACAAAATGGCCTCAAATCAATGGGACTGAGTTGGCTTGCCTCTTGGCTAAGTTCACTTTATGCAATGTCATTCAATTCTTCTGTGGAAGCTTCATTTGACTCAGGAGTAGAAATGGAATTCATAGAAAACCTTCGAAAAATTCCATTTATTCCTCTTTCAGACGGTACATATGTTGCAGTGGATAAAGGCCCAATTTGGTTACATTTTGATGCCTTAAAGACTGGGTTTGAGGGTCAGCATGGACTTGAGTCTTTTCCAGATTTGTATGCTAACCTTCAGATTGTAAGTCCTAGCTTCCTTTTTGCATCATGTGCTGACGTGTCATACATGAATGTGACGACAGTTGAAAAAGTCACAAGTATGCTTCGTAGAATTGGTGTCCAACAGTTGTCCGCACATGAAATTATCAAGGTGCATATCTTGCCGGCTATATCTGATGGCAGGATTACAGATAGGGATAAGAATTTGATGACTGATTATATTTGTTTTGTAATGGTTCACCTGCAGTCGAGCTGCTATACTTGTCAAGTTGAAAAGGAATACATAATCTCAGAACTACGAAATAAAGCTTATATACTAACAAATAATGGCTTTAAACGACCTGCTGAAGCATCAGTTCATTTCAGTGCAGCATTTGGAAATCCTGTGGACATAAATAATTTAATTAATGGGGTGGATATGGTTTGGCATGAGGTTGACATCTCCTATTTGAAGCATCCCACCACTAGATCACTCCCAAATGGACTGATGAAGTGGAGGGAGTTTTTCCAGAAAATTGGCATAACGGATTTTGTGAAAGTAGTTCAAGTTGAGAAAGGAATTAATAGTTTATCTGATTCTTTTTTGAAGAAGTTGATTTGGGACAAGGACAGCATTTCCCTTGGACTGAATGCCACAGATTGGGAGTCACCTGAATTAGTCCAACTATTATCCCTATTGTCCAGAGAGAACAACAAAAAAGGTTGTGAATATCTCCTAGAGGTTCTTGATACATTATGGGATAATTGCTATAGCGATAAAACGACCGGTTACTGCACTTCTAAATCTGTAGCAGATAGACAGCCCTTCAGATCTTCATTTATAAGCTGCATCTGTGATGCGCAATGGGTGGTATCAACAATGGATGATGAACTTCACTACCCCAAGGATTTATATCACAACTGTGATCCAGTGCGCTCGATTCTGGGTACATCTGCTCCATGTTCTGTTCCAAAGGTGAGAAGTGGAAAGTTCGCAAGTGATATTGGGTTTAAGACGAGAGTTTCTCTTGGTGATGTTTTTGAGATTTTGAAACTATGGAGATGTGAGAACCCATTCAGAGCAAGCCTAGCACAAATGTTCAAAATTTACTCATTAATCTGGAATGAAATGGCTCCTTCGAATCAGAGAATTACCGATGAGTTCCATCCTGGACCTTTCATATTTGTTCCATATGAATCCAGCTTCAGTCATGAGGATGTGGTGTCTGGTATATTTTTGTCCCCTGAAGAAGTATATTGGGATGATTCCACTTCCTTCGTTAACCATATCAAGGGGATCCGTCCTCAGTACAGCTCAACAGGTATTAATCATATTCCCTTAAATAAGATGCTGAGTAATTTCTACCCAGGCCTTCACGACTTCTTTGTTGGTGGGTGTGGAGTACATGAGATCCCAGCTCTCCGTAGCTACCTTCGGGTTCTGCTAGATTTATCAAATATTGCTTTGCCTTCACAAGCAGCTAATGCCGTTTTTCGAGTATTCCTGAAATGGACTGATGGACTGAAGTCAGGATTGAGTGCTGAGGATATTGTTTACTTGAAAGATTCTCTTACAAATATTGAATGTACGGTGCTTCCTACTGTTCAAGATAAGTGGGTTTCTCTACATCCTTCCTTTGGTCATGTGTTCTGGTGTGATGATATTGAGTTAAGAAAGCAGTTTATGCATTTGGATGGCGTTGATTTTCTATACTTTGGTGAACTAAGTAATGCTGATGTAGAGATGCTATTTAAAAAAGTTTCCATTCTCCTGAAAGCTTTAGGGATTCCTGCACTTTCAGAGGCTGTAACTCGTGAGGCAATATTTTATGGTCTGGCAGATTGTAGCACAAAAGCTGCATTGATGGATTGGGCTCTTCCATATGTACAGCGCTACTTACAAAGTGTACACCCTGATAAATATGCACAACTCAAGCAGTCTGGATTTGATATCTTAAATCGTTTGCAAGTAGTTGTTGTTGAAAAGCTGTTTTATCAGAATGTTATAAAGAGTTCCGGCAGGAAATCCAATAAGAGGCTAAGATGTAGCTGTCTTCTGCAGGGTAGCAATTTGTATACAACCCAAGAACCGGATTCACACGCGTTATTTGTGGAGCTTTCTCGTTTGTTTTTTGACGGAAATCCAGAATTACACTTGGCAAATTTCCTTCATATGATCACAATAATGGCTGAATCAGGTTCAACTCAGGAGCAGACAGAATTTTTTATCTTAAACAGCCAAAAGGTACCGAAGCTTTCTGGTGAAGAATCTATTTGGTCCCTGTCATCTGTGACTTCATTGACAGACAATTATAAGTCACTCCCAAAAAGTTTTACTTTTACAGAAGTAAATGAGAAAAACAGTTGGGAATCCAAAAGGAAAGTAATTAACTGGCCACCTATTGATTGGAAAACTGCACCAGGTTTTGGTTGTACTGATGCAAATCTTTCCAAGACACAGGCACCAGTTGCACATTCTGTTTCAGTTGACATTAATTGGACCATTGAAGATGATTCAGCAATGACATCTGCAGCTTTAGTTTTGCCAGGCTCCGATGATCTGCAAGAACATTCTGGTTATGCTTGCAATGAGACTGGAAATGGAATGCATACTGAATTTGATCCTATTAATGTAGGTTTTGTTTCTGACTCTCCTGATTTGGGTTCATTTCCTAATTTTAGCAAGAGAGACCAGCCTAGATATGGTACATCCAGTGGAAGAGATGCAATGCTAACCGGGAGACTGGGTGAGCTTGTTGCTTTCAAATACTTGATTTCAAAAGCTGGAAAGTCAGTTGTGAAGTGGGTTAATGAATGCAATGAAACTGGGCTTCCCTATGACATAGTTGTAGGGGAGAAGGAAGACAGTACAGAGTTCATTGAAGTTAAAGCAACCCAGTCTCCTAGAAAGGACTGGTTCCACATATCAATGAGAGAGTGGCAATTTGCAGTAGATAAAGGTGAAGCCTTCAGCATTTTGCATGTCATTTTATTGGGTAATAATGCTGCAAAGGTCTCAGTATACAAGAATCCTGTACAATTGTGCATGCTGGGGAAGCTGAAGTTGCATCTTATGATGCCTAAGCAACAGAACGAATTATTCCTTTTGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

2274

Amino Acids

254.5

Weight (kDa)

5.76

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 4550, 5714, 6021
AarI CACCTGC 1 cut(s) 3978
Acc16I TGCGCA 1 cut(s) 4569
Acc36I ACCTGC 3 cut(s) 1600, 3978, 4078
Acc65I GGTACC 2 cut(s) 243, 5953
AccB1I GGYRCC 3 cut(s) 243, 5953, 6169
AccB7I CCANNNNNTGG 4 cut(s) 235, 830, 4368, 6134
AccI GTMKAC 4 cut(s) 646, 1407, 5783, 6729
AccII CGCG 2 cut(s) 3205, 5810
AccIII TCCGGA 1 cut(s) 113
AciI CCGC 8 cut(s) 64, 1881, 2131, 2564, 2692, 3164, 3203, 3861
AcoI YGGCCR 2 cut(s) 1018, 6101
AdeI CACNNNGTG 2 cut(s) 44, 2333
AfeI AGCGCT 1 cut(s) 5600
AflIII ACRYGT 5 cut(s) 166, 913, 3226, 3786, 5808
AgeI ACCGGT 1 cut(s) 4493
AhlI ACTAGT 2 cut(s) 944, 1493
AjiI CACGTC 3 cut(s) 41, 169, 3787
AjnI CCWGG 9 cut(s) 720, 2538, 3038, 3067, 3246, 4876, 5074, 6127, 6253
AjuI GAANNNNNNNTTGG 6 cut(s) 686, 718, 1881, 1913, 1971, 2003
AleI CACNNNNGTG 2 cut(s) 1001, 4629
Alw21I GWGCWC 4 cut(s) 308, 998, 2146, 3106
Alw26I GTCTC 6 cut(s) 1706, 6293, 6390, 6442, 6612, 6727
AlwNI CAGNNNCTG 1 cut(s) 5513
Ama87I CYCGRG 1 cut(s) 3128
Aor13HI TCCGGA 1 cut(s) 113
Aor51HI AGCGCT 1 cut(s) 5600
AoxI GGCC 5 cut(s) 1018, 3475, 3655, 5077, 6101
ArsI GACNNNNNNTTYG 2 cut(s) 1112, 1144
AseI ATTAAT 7 cut(s) 2646, 4134, 4290, 4823, 5034, 6201, 6336
AsiGI ACCGGT 1 cut(s) 4493
Asp700I GAANNNNTTC 5 cut(s) 818, 867, 3270, 3597, 4781
Asp718I GGTACC 2 cut(s) 243, 5953
AspLEI GCGC 3 cut(s) 4570, 4644, 5601
AsuC2I CCSGG 5 cut(s) 1531, 1837, 3129, 3130, 6445
AsuII TTCGAA 2 cut(s) 3601, 4847
AvaI CYCGRG 1 cut(s) 3128
AvaII GGWCC 9 cut(s) 70, 344, 1082, 1120, 3286, 3425, 4880, 5985, 6207
BaeGI GKGCMC 3 cut(s) 701, 1114, 2329
BaeI ACNNNNGTAYC 8 cut(s) 1137, 1137, 1170, 1170, 2490, 2523, 3624, 3657
BalI TGGCCA 2 cut(s) 1020, 6103
BamHI GGATCC 2 cut(s) 3388, 5004
BanI GGYRCC 3 cut(s) 243, 5953, 6169
BanII GRGCYC 2 cut(s) 2146, 5584
BauI CACGAG 1 cut(s) 5520
BbsI GAAGAC 4 cut(s) 358, 450, 5753, 6576
Bbv12I GWGCWC 4 cut(s) 308, 998, 2146, 3106
BbvCI CCTCAGC 1 cut(s) 5250
BceAI ACGGC 1 cut(s) 5180
BciT130I CCWGG 9 cut(s) 722, 2540, 3040, 3069, 3248, 4878, 5076, 6129, 6255
BclI TGATCA 3 cut(s) 414, 481, 5883
BcnI CCSGG 5 cut(s) 1531, 1837, 3129, 3130, 6445
BcoDI GTCTC 6 cut(s) 1706, 6293, 6390, 6442, 6612, 6727
BcuI ACTAGT 2 cut(s) 944, 1493
BfmI CTRYAG 9 cut(s) 348, 1245, 1593, 2010, 3971, 4477, 4515, 5765, 6237
BfoI RGCGCY 1 cut(s) 5602
BfuAI ACCTGC 3 cut(s) 1600, 3978, 4078
BglII AGATCT 4 cut(s) 2606, 2659, 3092, 4538
Bme18I GGWCC 9 cut(s) 70, 344, 1082, 1120, 3286, 3425, 4880, 5985, 6207
BmeT110I CYCGRG 1 cut(s) 3128
BmgBI CACGTC 3 cut(s) 41, 169, 3787
BmrI ACTGGG 4 cut(s) 3699, 6461, 6543, 6598
BmuI ACTGGG 4 cut(s) 3699, 6461, 6543, 6598
BoxI GACNNNNGTC 2 cut(s) 3715, 5233
BpiI GAAGAC 4 cut(s) 358, 450, 5753, 6576
BplI GAGNNNNNCTC 2 cut(s) 4717, 4749
BpmI CTGGAG 2 cut(s) 2412, 2681
Bpu10I CCTNAGC 4 cut(s) 809, 2134, 5250, 6789
Bpu14I TTCGAA 2 cut(s) 3601, 4847
BpuEI CTTGAG 4 cut(s) 732, 2216, 3734, 5624
BpuMI CCSGG 5 cut(s) 1531, 1837, 3129, 3130, 6445
Bsa29I ATCGAT 1 cut(s) 827
BsaAI YACGTR 1 cut(s) 3227
BsaBI GATNNNNATC 4 cut(s) 527, 2664, 4620, 6150
BsaI GGTCTC 2 cut(s) 6390, 6727
BsaJI CCNNGG 8 cut(s) 601, 720, 1157, 3038, 3128, 4348, 4611, 5074
BsaWI WCCGGW 4 cut(s) 113, 377, 4493, 5797
Bse118I RCCGGY 2 cut(s) 3892, 4493
Bse3DI GCAATG 7 cut(s) 2149, 3537, 4577, 6234, 6301, 6441, 6532
Bse8I GATNNNNATC 4 cut(s) 527, 2664, 4620, 6150
BseAI TCCGGA 1 cut(s) 113
BseBI CCWGG 9 cut(s) 722, 2540, 3040, 3069, 3248, 4878, 5076, 6129, 6255
BseCI ATCGAT 1 cut(s) 827
BseDI CCNNGG 8 cut(s) 601, 720, 1157, 3038, 3128, 4348, 4611, 5074
BseJI GATNNNNATC 4 cut(s) 527, 2664, 4620, 6150
BseMI GCAATG 7 cut(s) 2149, 3537, 4577, 6234, 6301, 6441, 6532
BseRI GAGGAG 3 cut(s) 396, 1270, 2502
BseSI GKGCMC 3 cut(s) 701, 1114, 2329
BseYI CCCAGC 3 cut(s) 291, 5122, 6757
BsgI GTGCAG 6 cut(s) 1986, 3301, 4116, 4486, 5484, 6108
Bsh1236I CGCG 2 cut(s) 3205, 5810
Bsh1285I CGRYCG 1 cut(s) 4494
BshFI GGCC 5 cut(s) 1020, 3477, 3657, 5079, 6103
BshNI GGYRCC 3 cut(s) 243, 5953, 6169
BshTI ACCGGT 1 cut(s) 4493
BshVI ATCGAT 1 cut(s) 827
BsiEI CGRYCG 1 cut(s) 4494
BsiHKAI GWGCWC 4 cut(s) 308, 998, 2146, 3106
BsiHKCI CYCGRG 1 cut(s) 3128
BslFI GGGAC 9 cut(s) 274, 1095, 1133, 1336, 3021, 3504, 4343, 4933, 5971
BsmAI GTCTC 6 cut(s) 1706, 6293, 6390, 6442, 6612, 6727
BsmBI CGTCTC 1 cut(s) 1706
BsmFI GGGAC 9 cut(s) 274, 1095, 1133, 1336, 3021, 3504, 4343, 4933, 5971
BsmI GAATGC 7 cut(s) 495, 1012, 1253, 2005, 4363, 6318, 6527
BsnI GGCC 5 cut(s) 1020, 3477, 3657, 5079, 6103
Bso31I GGTCTC 2 cut(s) 6390, 6727
BsoBI CYCGRG 1 cut(s) 3128
Bsp119I TTCGAA 2 cut(s) 3601, 4847
Bsp1286I GDGCHC 8 cut(s) 308, 701, 998, 1114, 2146, 2329, 3106, 5584
Bsp13I TCCGGA 1 cut(s) 113
Bsp1407I TGTACA 5 cut(s) 1369, 2854, 5593, 5614, 6742
Bsp19I CCATGG 1 cut(s) 1157
BspACI CCGC 8 cut(s) 64, 1881, 2131, 2564, 2692, 3164, 3203, 3861
BspANI GGCC 5 cut(s) 1020, 3477, 3657, 5079, 6103
BspDI ATCGAT 1 cut(s) 827
BspEI TCCGGA 1 cut(s) 113
BspFNI CGCG 2 cut(s) 3205, 5810
BspHI TCATGA 6 cut(s) 1704, 1711, 1750, 2631, 2742, 4918
BspMAI CTGCAG 4 cut(s) 1597, 3975, 5769, 6241
BspMI ACCTGC 3 cut(s) 1600, 3978, 4078
BspQI GCTCTTC 2 cut(s) 3111, 5589
BspT104I TTCGAA 2 cut(s) 3601, 4847
BspT107I GGYRCC 3 cut(s) 243, 5953, 6169
BspTNI GGTCTC 2 cut(s) 6390, 6727
BsrDI GCAATG 7 cut(s) 2149, 3537, 4577, 6234, 6301, 6441, 6532
BsrFI RCCGGY 2 cut(s) 3892, 4493
BsrGI TGTACA 5 cut(s) 1369, 2854, 5593, 5614, 6742
BssAI RCCGGY 2 cut(s) 3892, 4493
BssECI CCNNGG 8 cut(s) 601, 720, 1157, 3038, 3128, 4348, 4611, 5074
BssNAI GTATAC 2 cut(s) 5784, 6730
BssSI CACGAG 1 cut(s) 5520
BssT1I CCWWGG 4 cut(s) 601, 1157, 4348, 4611
Bst1107I GTATAC 2 cut(s) 5784, 6730
Bst2BI CACGAG 1 cut(s) 5520
Bst2UI CCWGG 9 cut(s) 722, 2540, 3040, 3069, 3248, 4878, 5076, 6129, 6255
Bst6I CTCTTC 5 cut(s) 390, 3111, 3204, 5589, 6421
BstAPI GCANNNNNTGC 5 cut(s) 3779, 3889, 5192, 5392, 6176
BstAUI TGTACA 5 cut(s) 1369, 2854, 5593, 5614, 6742
BstBAI YACGTR 1 cut(s) 3227
BstBI TTCGAA 2 cut(s) 3601, 4847
BstDSI CCRYGG 1 cut(s) 1157
BstEII GGTNACC 1 cut(s) 1201
BstENI CCTNNNNNAGG 1 cut(s) 2159
BstFNI CGCG 2 cut(s) 3205, 5810
BstH2I RGCGCY 1 cut(s) 5602
BstHHI GCGC 3 cut(s) 4570, 4644, 5601
BstMAI GTCTC 6 cut(s) 1706, 6293, 6390, 6442, 6612, 6727
BstMCI CGRYCG 1 cut(s) 4494
BstNI CCWGG 9 cut(s) 722, 2540, 3040, 3069, 3248, 4878, 5076, 6129, 6255
BstNSI RCATGY 8 cut(s) 718, 1343, 1379, 1658, 1812, 2129, 6692, 6757
BstPAI GACNNNNGTC 2 cut(s) 3715, 5233
BstPI GGTNACC 1 cut(s) 1201
BstSFI CTRYAG 9 cut(s) 348, 1245, 1593, 2010, 3971, 4477, 4515, 5765, 6237
BstSLI GKGCMC 3 cut(s) 701, 1114, 2329
BstUI CGCG 2 cut(s) 3205, 5810
BstV2I GAAGAC 4 cut(s) 358, 450, 5753, 6576
BstX2I RGATCY 9 cut(s) 119, 2360, 2606, 2659, 3092, 3388, 4538, 5004, 5118
BstXI CCANNNNNNTGG 1 cut(s) 4244
BstYI RGATCY 9 cut(s) 119, 2360, 2606, 2659, 3092, 3388, 4538, 5004, 5118
BstZ17I GTATAC 2 cut(s) 5784, 6730
Bsu15I ATCGAT 1 cut(s) 827
BsuRI GGCC 5 cut(s) 1020, 3477, 3657, 5079, 6103
BsuTUI ATCGAT 1 cut(s) 827
BtgI CCRYGG 1 cut(s) 1157
BtgZI GCGATG 1 cut(s) 404
BtrI CACGTC 3 cut(s) 41, 169, 3787
BtsI GCAGTG 4 cut(s) 897, 1302, 1999, 3651
BtsIMutI CAGTG 9 cut(s) 897, 970, 1302, 1999, 2705, 3651, 4099, 4644, 6427
BveI ACCTGC 3 cut(s) 1600, 3978, 4078
CaiI CAGNNNCTG 1 cut(s) 5513
CciI TCATGA 6 cut(s) 1704, 1711, 1750, 2631, 2742, 4918
CfoI GCGC 3 cut(s) 4570, 4644, 5601
Cfr10I RCCGGY 2 cut(s) 3892, 4493
Cfr9I CCCGGG 1 cut(s) 3128
ClaI ATCGAT 1 cut(s) 827
CseI GACGC 1 cut(s) 1688
CsiI ACCWGGT 1 cut(s) 6127
CspAI ACCGGT 1 cut(s) 4493
CspCI CAANNNNNGTGG 4 cut(s) 2220, 2255, 5303, 5338
DraI TTTAAA 6 cut(s) 756, 1101, 2289, 3118, 4063, 5461
DraIII CACNNNGTG 2 cut(s) 44, 2333
EaeI YGGCCR 2 cut(s) 1018, 6101
Eam1104I CTCTTC 5 cut(s) 390, 3111, 3204, 5589, 6421
EarI CTCTTC 5 cut(s) 390, 3111, 3204, 5589, 6421
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 2144
Eco130I CCWWGG 4 cut(s) 601, 1157, 4348, 4611
Eco147I AGGCCT 1 cut(s) 5079
Eco24I GRGCYC 2 cut(s) 2146, 5584
Eco31I GGTCTC 2 cut(s) 6390, 6727
Eco32I GATATC 1 cut(s) 5659
Eco47I GGWCC 9 cut(s) 70, 344, 1082, 1120, 3286, 3425, 4880, 5985, 6207
Eco47III AGCGCT 1 cut(s) 5600
Eco53kI GAGCTC 1 cut(s) 2144
Eco88I CYCGRG 1 cut(s) 3128
Eco91I GGTNACC 1 cut(s) 1201
EcoICRI GAGCTC 1 cut(s) 2144
EcoNI CCTNNNNNAGG 1 cut(s) 2159
EcoO109I RGGNCCY 3 cut(s) 70, 1120, 3286
EcoO65I GGTNACC 1 cut(s) 1201
EcoRI GAATTC 1 cut(s) 3584
EcoRII CCWGG 9 cut(s) 720, 2538, 3038, 3067, 3246, 4876, 5074, 6127, 6253
EcoRV GATATC 1 cut(s) 5659
EcoT14I CCWWGG 4 cut(s) 601, 1157, 4348, 4611
EcoT22I ATGCAT 4 cut(s) 1255, 1381, 5397, 6318
EcoT38I GRGCYC 2 cut(s) 2146, 5584
ErhI CCWWGG 4 cut(s) 601, 1157, 4348, 4611
Esp3I CGTCTC 1 cut(s) 1706
FaqI GGGAC 9 cut(s) 274, 1095, 1133, 1336, 3021, 3504, 4343, 4933, 5971
FauI CCCGC 1 cut(s) 2138
FauNDI CATATG 5 cut(s) 2965, 3637, 4900, 5590, 5880
FbaI TGATCA 3 cut(s) 414, 481, 5883
FblI GTMKAC 4 cut(s) 646, 1407, 5783, 6729
FriOI GRGCYC 2 cut(s) 2146, 5584
FspI TGCGCA 1 cut(s) 4569
GlaI GCGC 3 cut(s) 4569, 4643, 5600
GsaI CCCAGC 3 cut(s) 295, 5126, 6761
GsuI CTGGAG 2 cut(s) 2412, 2681
HaeII RGCGCY 1 cut(s) 5602
HaeIII GGCC 5 cut(s) 1020, 3477, 3657, 5079, 6103
HgaI GACGC 1 cut(s) 1688
HhaI GCGC 3 cut(s) 4570, 4644, 5601
Hin6I GCGC 3 cut(s) 4568, 4642, 5599
HinP1I GCGC 3 cut(s) 4568, 4642, 5599
HincII GTYRAC 5 cut(s) 1177, 3082, 4165, 4990, 6196
HindII GTYRAC 5 cut(s) 1177, 3082, 4165, 4990, 6196
HindIII AAGCTT 7 cut(s) 542, 750, 1971, 3555, 4035, 5484, 5960
HpaI GTTAAC 2 cut(s) 3082, 4990
Hpy99I CGWCG 2 cut(s) 45, 469
HpyCH4IV ACGT 6 cut(s) 40, 168, 467, 913, 3226, 3786
HpySE526I ACGT 6 cut(s) 40, 168, 467, 913, 3226, 3786
HspAI GCGC 3 cut(s) 4568, 4642, 5599
Kpn2I TCCGGA 1 cut(s) 113
KpnI GGTACC 2 cut(s) 247, 5957
KroI GCCGGC 1 cut(s) 3892
KroNI GCCGGC 1 cut(s) 3894
Ksp22I TGATCA 3 cut(s) 414, 481, 5883
KspAI GTTAAC 2 cut(s) 3082, 4990
LguI GCTCTTC 2 cut(s) 3111, 5589
MabI ACCWGGT 1 cut(s) 6127
MaeII ACGT 6 cut(s) 40, 168, 467, 913, 3226, 3786
MfeI CAATTG 2 cut(s) 550, 6746
MflI RGATCY 9 cut(s) 119, 2360, 2606, 2659, 3092, 3388, 4538, 5004, 5118
MhlI GDGCHC 8 cut(s) 308, 701, 998, 1114, 2146, 2329, 3106, 5584
MlsI TGGCCA 2 cut(s) 1020, 6103
MluI ACGCGT 1 cut(s) 5808
MluNI TGGCCA 2 cut(s) 1020, 6103
MmeI TCCRAC 6 cut(s) 217, 2080, 2320, 3278, 3874, 4414
Mox20I TGGCCA 2 cut(s) 1020, 6103
Mph1103I ATGCAT 4 cut(s) 1255, 1381, 5397, 6318
MroI TCCGGA 1 cut(s) 113
MroNI GCCGGC 1 cut(s) 3892
MroXI GAANNNNTTC 5 cut(s) 818, 867, 3270, 3597, 4781
MscI TGGCCA 2 cut(s) 1020, 6103
Msp20I TGGCCA 2 cut(s) 1020, 6103
MunI CAATTG 2 cut(s) 550, 6746
Mva1269I GAATGC 7 cut(s) 495, 1012, 1253, 2005, 4363, 6318, 6527
MvaI CCWGG 9 cut(s) 722, 2540, 3040, 3069, 3248, 4878, 5076, 6129, 6255
MvnI CGCG 2 cut(s) 3205, 5810
NaeI GCCGGC 1 cut(s) 3894
NciI CCSGG 5 cut(s) 1531, 1837, 3129, 3130, 6445
NcoI CCATGG 1 cut(s) 1157
NdeI CATATG 5 cut(s) 2965, 3637, 4900, 5590, 5880
NgoMIV GCCGGC 1 cut(s) 3892
NsbI TGCGCA 1 cut(s) 4569
NsiI ATGCAT 4 cut(s) 1255, 1381, 5397, 6318
NspI RCATGY 8 cut(s) 718, 1343, 1379, 1658, 1812, 2129, 6692, 6757
NspV TTCGAA 2 cut(s) 3601, 4847
OliI CACNNNNGTG 2 cut(s) 1001, 4629
PacI TTAATTAA 1 cut(s) 4134
PaeI GCATGC 4 cut(s) 718, 1379, 2129, 6757
PagI TCATGA 6 cut(s) 1704, 1711, 1750, 2631, 2742, 4918
PaqCI CACCTGC 1 cut(s) 3978
PceI AGGCCT 1 cut(s) 5079
PciSI GCTCTTC 2 cut(s) 3111, 5589
PctI GAATGC 7 cut(s) 495, 1012, 1253, 2005, 4363, 6318, 6527
PdiI GCCGGC 1 cut(s) 3894
PdmI GAANNNNTTC 5 cut(s) 818, 867, 3270, 3597, 4781
PflMI CCANNNNNTGG 4 cut(s) 235, 830, 4368, 6134
PfoI TCCNGGA 2 cut(s) 3246, 4876
PinAI ACCGGT 1 cut(s) 4493
Ppu21I YACGTR 1 cut(s) 3227
PpuMI RGGWCCY 3 cut(s) 70, 1120, 3286
PshAI GACNNNNGTC 2 cut(s) 3715, 5233
PshBI ATTAAT 7 cut(s) 2646, 4134, 4290, 4823, 5034, 6201, 6336
PsiI TTATAA 3 cut(s) 4550, 5714, 6021
Psp124BI GAGCTC 1 cut(s) 2146
Psp5II RGGWCCY 3 cut(s) 70, 1120, 3286
Psp6I CCWGG 9 cut(s) 720, 2538, 3038, 3067, 3246, 4876, 5074, 6127, 6253
PspEI GGTNACC 1 cut(s) 1201
PspFI CCCAGC 3 cut(s) 291, 5122, 6757
PspGI CCWGG 9 cut(s) 720, 2538, 3038, 3067, 3246, 4876, 5074, 6127, 6253
PspPPI RGGWCCY 3 cut(s) 70, 1120, 3286
PstI CTGCAG 4 cut(s) 1597, 3975, 5769, 6241
PstNI CAGNNNCTG 1 cut(s) 5513
PsuI RGATCY 9 cut(s) 119, 2360, 2606, 2659, 3092, 3388, 4538, 5004, 5118
SacI GAGCTC 1 cut(s) 2146
SapI GCTCTTC 2 cut(s) 3111, 5589
SduI GDGCHC 8 cut(s) 308, 701, 998, 1114, 2146, 2329, 3106, 5584
SexAI ACCWGGT 1 cut(s) 6127
SfcI CTRYAG 9 cut(s) 348, 1245, 1593, 2010, 3971, 4477, 4515, 5765, 6237
SfuI TTCGAA 2 cut(s) 3601, 4847
SinI GGWCC 9 cut(s) 70, 344, 1082, 1120, 3286, 3425, 4880, 5985, 6207
SmaI CCCGGG 1 cut(s) 3130
SmlI CTYRAG 4 cut(s) 747, 2195, 3713, 5639
SmoI CTYRAG 4 cut(s) 747, 2195, 3713, 5639
SpeI ACTAGT 2 cut(s) 944, 1493
SphI GCATGC 4 cut(s) 718, 1379, 2129, 6757
SseBI AGGCCT 1 cut(s) 5079
SsiI CCGC 8 cut(s) 64, 1881, 2131, 2564, 2692, 3164, 3203, 3861
SspI AATATT 6 cut(s) 638, 796, 2224, 5167, 5287, 5532
SstI GAGCTC 1 cut(s) 2146
StuI AGGCCT 1 cut(s) 5079
StyI CCWWGG 4 cut(s) 601, 1157, 4348, 4611
TaiI ACGT 6 cut(s) 43, 171, 470, 916, 3229, 3789
TaqI TCGA 9 cut(s) 827, 846, 3024, 3233, 3601, 3977, 4646, 4847, 5203
TauI GCSGC 1 cut(s) 1883
TscAI CASTG 9 cut(s) 904, 977, 1302, 1999, 2705, 3651, 4099, 4644, 6427
TspMI CCCGGG 1 cut(s) 3128
TspRI CASTG 9 cut(s) 904, 977, 1302, 1999, 2705, 3651, 4099, 4644, 6427
Van91I CCANNNNNTGG 4 cut(s) 235, 830, 4368, 6134
VpaK11BI GGWCC 9 cut(s) 70, 344, 1082, 1120, 3286, 3425, 4880, 5985, 6207
VspI ATTAAT 7 cut(s) 2646, 4134, 4290, 4823, 5034, 6201, 6336
XagI CCTNNNNNAGG 1 cut(s) 2159
XbaI TCTAGA 2 cut(s) 2077, 3011
XceI RCATGY 8 cut(s) 718, 1343, 1379, 1658, 1812, 2129, 6692, 6757
XcmI CCANNNNNNNNNTGG 2 cut(s) 6111, 6407
XmaI CCCGGG 1 cut(s) 3128
XmiI GTMKAC 4 cut(s) 646, 1407, 5783, 6729
XmnI GAANNNNTTC 5 cut(s) 818, 867, 3270, 3597, 4781
Zsp2I ATGCAT 4 cut(s) 1255, 1381, 5397, 6318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.