Rw4G015860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000582
Physical Location & Seq
Forward (+)
36322509 .. 36343987
21479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G015860.1

Sequence Viewer

Length: 8544 bp
ATGGCCGGTTACCCTCATCACTTCCGCCCCTCCAGTGGGGACTGGAACTGGGCACACCCACCTCCTCACCTCTCCCACCCACAATTTCCTTACCCCAATTTTCCTCCCCAAAACCCCACCAATTTAAACATCTATCCCCCTCAACCTTCCGCCTTTCCACCACCGCAACCTCAACCGCCGCAACCGTCAAACCCTAATTTCCCCCTCCAACACCCTATAGACGTCAATATCTTTCCCGCCAACCACTATGGACACTCCTCATTTTCCAATTACTACTCAACCCCAACCCCAAGCTCTAATTTCTCTCTGCAAAACCCTAACCCCACTCCCCACTCCACTCCACCCTTCCCCCCTCAGAGCTTCTCCCGTAACCCTAACCAGGCGCCCGACCCGCCGAACCTCCCGAATTCGGCTAGGCATTTGCCTGAGGACCCGAGAGAGGTGCACGAGAAGATCGACCGTGCGGTGGAGAGGCTTCGCGACGAGCTTGTCGCCGCCGGGAAGAACGTCTCGGCCTGGAAGGTGTTACAGTCTGCGCTTCTGATGCTCAATGTTGATGCCGAGACTTGTTTGGGGCTCAAAGTTGAGCAAGTGCCGTCTCTTCACCGCCTCATGGTCACTGAAGGAAGGATAAATGCATTTATTCATTGCTTTGTTGGAGTTCGAACAATTACATCTTTGTATGATTTGGAAGTTGCAATCTGCAAGAATGAGGATATTGAGCAGTTTGAAGAGCTTGGATTGGGTCCTTTGTTGCAACACCCACTAGTTTTGCAATATTTCTCTGTGGATTCTGAAACAACTGAAGTCTTTAAGATATCCAGTGAGGAGATACTACATTTACTCAGTAGGTACCTAAAGAAGCCCAAAGTTAAGGAAAATTCTGTTGAGGGGTTCTTGGACTTCATTGTTACGAAGCGACCTGTTGCAGGCAAGGAAAAGCTTGGAATTCGAATATTTAGTTTGGGGGTGCATGCTTCTGCTATGCGTGAAGCCAAGGCTTCAGAACGTGCAGCTTTATGGAAATCATTACAGGAATTAAAGAAAAAGTCTCCCAAAATATCTAGGAGGAAGGCACCAATTTTCTCCTCACTGAAAAAGCGGCTAGATAAACATTTTCGCGCCATTTCACAGCAATCTGGATTGTGTTCTGTTGTGAATGGAAATGAAGATACACTTGTTTCTTCAAGATCAAAGGATGAAGGTATTAATGATTATTTACATGATGACAAAGGTAATGACCATGGTACTGGTGGTCATATTGACTCACTAAAATCCCTGAAAAGTTCTGACAGAAAGGGTAAGTGTTCTTACTTGTCTGAAGTGGAGGAGAGGTCTTCGCTCATGTCAAACCACCCTTCTCCAAGCCAAAAGCATAATGAGAGTAATGGGTCAGTTAAAAAGAAAAGAAAATACGAAAATCTAAGCTGCCCTATCTCTGTTCCCTCAAAGTTGCGCAAGAGAGAGAAGGTAGAGCAAGATGCTCTTCCTACAGAAAGTGACAGTGAAACTAAGGAGATCAGCGAGATGCTTAAACTTGATCTTTCAATTGCCAATAATCTTTTGAGGATGTTCATTACAACTTGGAAGGAGGCATGTAGGGAGCAGCCTGTGGCTGAGGTACTTTGGGCCATGCTTCGCTCTTGTAATACAAAATATAGGAGGACAAAGAGAATCATGGCAATGTTTTCGACATACCCTTTGGTTGGATTACTCAATGTTGCCGTCTCATCTATCAAATGTGGAATGTGGGATAATATGTTTGATACGTCCCAAACCATTAGTCCACGTGAGTTGGCTAACACTGTAACTGATAATTATCCTGAACATGTCGAACAAAGTATAAAGGATGCACCACCAGTCAGCACCGAACATGCTACCGAACATGGACAAACCTCTTGCTGCCTGAAGCCAAATCATCAGGATCGTCCACGTCGGTGGAGGAAGATCCCAAGGAGTCGTAAGAAGTTGGAGCCTGAAAACGGACACATCTGCCACCTTTGTCATCAAGAGGGACATGTCAAGAGAAGATGCCCTCTGTGGCAGCAAACTAAAAAATCATCATATGAACCACAGAGTCCTCAACAATTGGCTCAGTATTCTATTTCCGGAGAAGAGGTGGAGGAGAAAGTCAGTGCTGTGGTGGATGAGTCCACAGGTGTTTCAGTTAAAGAGATAATCCGGAAAGTTGCTACATATTTTGAGACTGATCAAGGAATGTCTAGCAATGGTAAATCACTTCCGGAGAAGATATTTATTTACTTCAGAAAGCTTTGGAATTGTGAGCTCTGGTTGACTGAAGAATTTTCTGTCAAGGAATTTAGATCCCTTGGTTATGGGGAATTTATGATGTTTTTAGAAAAGTATGCCCGTCTTCTGCCACCGGAGATGTGCAAGTTCTTGAAAGATGACAGCAGTGGAAAGTTTCCTTTGGAGGTTTGCATGCTTCCGCATTATTTAGTTGTTTTGGTATCTCAAGCTTTAAACAATTTATGGGAGGACCAAGAGATTACCAAACTGAACATTTCTTCTCTGCTTGGGAAGCAGTTCCCGGCCCTTAGTTTCCATACCATTGAAAATGGTTCAGTAGAAGACTTTAAAAGTATAGTAGGAAAGCATAAAGACACTGCAATTTCCAAATGTGTACTATTTTCAATGACAATATGCGGAACTAGTTACACTATAGAATCATTGGAAAACTGTAGTAAGAATGCTGTATTGAAAAGCAGTTTGGTGAGTGTTAACAGTGGCCAGAAAATAAGCAATCAATCTGTTGCATCCAAGGAGGCAATTGCAGTCTTACTTAGGGCACCCATGATGTCAGATTTGAATTTATGGTCACATTGGGACCTCTTATTTTCTCCCTCTCTTGGCCCTCTTGTACCGTGGTTATTGAATGAAGTCAACACAGATGAATTACTCTGTTTGGTAACCAAAGATGGCAAAGTTATTCGGTTAGATCATTCAGCTACTGTAGATTCATTCTCGAAAGCTGCTCTTCAAGGATCCTCTTTTCAAACAGCAGTGAAAATGTTATCTCTCTTTTCACTGGTTGGGGGAAAAAAACATGTCCCCGTGTCCCTTTTGAAATTCCATGTACAGCGTGCATTTGAAGTTATTTTCAAGACCTATCTAGATGGTATGGAAGTATATGACAAGAAGAGTTCTCTTAATCAAGGAACAGTTTTGTGTGGACAGAAAATGGTTGTTGAAATTGCTGCTGGTAAATGCAGTAACTTACACAGAGCTGTAACGAAGACGAAAATTGCTGAGTCTGTTATATCACATTTTTTTCTTGATTGCCTGGGGTATCTACCTGCAGAGTTTCGCAGTTTTGCTGCTGATGTGTTACTTTCTGGGATGCGGTCTGTTTTTAAGCATGCAGCTTCAGCCATTTTGGATGAATGCATCCAAATAGAGCAGCGTCTCATACTTCATGAAGTTGGCTTATCTCTTGGTATAGTGGAGTGGATTAGTGATTACCATGTATTTAGTTCCTTGGATTCTACTGATTCGTTCATGTCTGAGGTTTCTGGTCTCAATGCTGATAGAGATCAAATGGGGTCAGACTCAAAATATATGCAAAATGTGTCAAAAAAGTTGGCTGCTTCTGAACAGAGTATGGCTGCATCTGTTAGGGCAGTTGAGCATAAAGGAGGATGTGCTGATGTTAGCCGGATGGTTGATGGTGCAGGAGTTTCTGATGCTGGGATTGGCATTGGCATTGGTTACAGACAACATCCTACTAATATTAATGAACATGAAGATGCTCTCCAGGTTATTGAGTCAATCAGGAGAGATGAATTCGGTTTGGATTCAAGCCCATTGACCTTTGAAAGTATCGTCTTAAAGAAACAGCATGCTCGCTTAGGGAGAGCTCTGCACTGTCTTTCACAGGAATTATATTCTCAAGATTCTCATTTTCTTCTTGAGCTGGTTCAAAATGCTGATGATAATACTTATCCAACAAATATAGAACCAACTCTAGCATTCATTCTTCAAGAATCAGGTATTGTTGTTTTGAATAATGAGCAAGGCTTCTCTGCCCAGAACATTAGAGCACTTTGTGATGTTGGAAATTCAACCAAGAAAGGATCCAGTGCCGGATATATAGGGCAAAAGGGCATCGGTTTCAAATCAGTATTTCGTGTCACAGATGCTCCAGAAATTCATTCTAATGGATTTCATATCAAGTTTGATACAAGTGAGGGTCAGATTGGTTTAATTCTGCCCACAGTTGTACCTCCTTGCAATCTTGAGTTGTTTAGACAGCTGACATCTAGTGACATTGATAAACCAGATTGTTACTGCTGGAAAACTTGTATTGTTCTTCCTTTCAGATCGAAAGTATCAGATGGAACTGTCTTGAACCACATAATGAATATGTTTTCAGATCTTCATCCATCTTTACTACTCTTTCTTCACCGCCTCAAGTGTATCCGGTTTAGAAACCTAATCGATGATTCACTTACTGTCATGAGAAAAGAAATTGTAGGAGACGGTATTGTCAAGGTTTCACATGGTAATACGAAGATGACATGGTTTGTAGTATCTCAAAAATTGCACTCAGATAATCTTCGTAGTGATGTGCAAACAACAGAAATCTCAATTGCTTTTACATTGAAGGAATTGGATAAGGGCATTTATCGTCCAGATATAGGCCAACAGCCTGTTTTTGCATTTCTTCCTCTTAGAACATACGGACTGAAATTTATTGTTCAGGGTGATTTTGTTCTTCCTTCATCCAGAGAGGAAGTGGATGGAGATAGTCCCTGGAACCAATGGCTATTGTCAGAATTTCCTGGTTTATTTGTCGATGCAGAGAAATCATTTGGTTCTCTTCCATGTTTCAAAGAGAATCCTGGGACTGCTGTGGCAGCTTACATGTCCTTTGTTCCACTTGTTGGGGAAGTGCATGGTTTCTTTTCTTCTCTTCCTCGATTAATTATTTCTAAATTAGGGATGTCAAATTGCTTACTTCAGGAAGGAGAAAAGAAAGAATGGGTTCCTCCTTGCAAGGTCCTAAGAAATTGGAATAAACAGGCTCGTTCACTTCTCCCTGATGGTCTACTGCATGAGCATCTTGGCCTTGGATTCTTGGATAAAGATATAGTTCTGCCTGATCCACTTGCAAGAGTTCTAGGTATTGCAGAGTATGGGCCCACAGTTCTGCTTCAGGTAATGGCTTCTTTATGTGGTAAGCAGGATGGCCTCAAATCAATGGGAATGGGTTGGATGGCCTCTTTGCTTAGTGAACTTTATGCAATGTCATTCAAATCTTCTGTTGAAACTTCCTTTGATTCTGGAATAGAAATGGGATTCCTAGAAAAACTTCAAAAAATTCCATTCATACCTCTTTCAGATGGTACATATGGTGCAGTCGTTGAAGGCCCAATTTGGCTACAGTTTGATGCCGTAGGGACTGGTTTTGGGGATCATCATGGACTTGAATCTTTTCCAAAGTTATATGCTAAACTTCGGATTGTAAGTCCGGAGCTCTATGCATCGTCTGCTGATATGCCATCCATGGATGTGACGCCAATTGACAAAGTCATTAGTATGCTCCATAGAATTGGTGTCCAACGGTTGTCCGCACACGAGATTCTCAAGGCTCACATCTTGCCAGCTATATCTGATGATAGAATTACAGTTAGGGATGAAGATTTGATGACTGATTATATCTGCTTCGCAATGGTTCACTTACAGTCGAGCTGCTCTGATTGTCATGCTGAAAGGGAATACATAATATCAGAACTACAAAATAAAGCTTATATTCTAACTAATAATGGCTTTAAACGACCTGCTGAGGCATCAATTCATTTCAGTAAAGAATTTGGAAATCCAGTGGACATAAGTAGGTTGATTGATAGGGTAAATCTGACATGGGATGAGGTTGATATCTCCTATTTGAAGCATCCAGTTGCTAAATCACTTCCAAATGGACTGATGAAGTGGAGAGATTTTTTCCAGAAAATTGGCATTGTGGATTTTGTGAAGGTAGTTCAAGTTGAGAAGGGTTTCACTGAGTTGTCTGAGGCTCTATTTAATAATCTAACTTGGGACCAGCATACTATTTCCCATGGGTTGAATGCCACAGATTGGGAATCACCTGAGTTAGTCCATTTATTATCCCTATTGTCCGGAGATGGCAACAGAAAAGGTTGTGAGTATCTCTTAGAGGTTCTCGATAAATTATGGGATGATTGCTATAGTGACAGAGCTACAGGGTACTGCTCTTCTAAGTCTGTTGCAGATAGAAAGCCCTTCAAATCTTCATTTATGAGCACCATCTGCGATGTGCAATGGGTAGTATCAGGAATGGATGATAAACTTCACTATCCCAAGGATCTATATCACGACAGTGTTGCAGTGCGGTCAATTCTTGGTGGATCTGCCCCTTATTTTGTTCCTAAGGCTACCAGTGAAAAATTTGTATTTGATATTGGTTTTAAGACAAGAGTCTCTCTTCACGATGCTCTGGAGATGTTGAAACTATGGAGATGTGAGAATCCGTTTAGGGCCAGCCTAGCACAAATGTCCAAATTTTACTCATTAATCTGGAATGAAATGGCTTCTTCAAAGAAGACAACTGCAGAGGAATTCTGTTTGGAACCTTTTGTATTTGTTCCATATGAATCTAGCTTCCGGCATGAGGATGTGGTGTCTGGTACATTTCTGTCCCTTGAAGAAGTATACTGGGATGATTCTACTTTCTTTGTGGACCAAATCAAGGAGATCCATCATCAGTGCAACTCAACAGTTGGTAATCGCCCCATAAACAGGATTCTGAGTAATTTCTACCCGGCCCTTCATGACTTCTTTGTTGATATATGTGGAGTACATGAGATCCCTCCTCTTCGTAGCTACCTTCAGATTTTGCTACAGTTTTCAAATGCTGTTTTGCCTTCACAAGCAGCTAATGCTGTTTTTCAAGTTTTTCAGAAGTGGGCTGATGGGCTCCAATCAGGAATGAGTGCTGAGGATATTGTATACTTGAAAGATTCGCTTACAAGGATGGAATGTACTGTGCTTCCTACTGTACAGGATAAGTGGGTATCTCTGCATCCTTCCTATGGTCTCCTGTGCTGGTGTGATGATAAGACGTTGAAGAAGCAGTTTATGCATATGGATGGACTTGATTTTCTTTACTTTGGTGGACTAAGTAATGATGATGAAGAGATCATAAGTACAAAAATGTCCGTCCTCATGCGAACCTTAGGGATTTCTGCACTTTCAGAGGTTGTGACTCGTGAGGCGATGTTTAATGGTCCGACAGATTCTAGCTTTAAAGCTGCATTGCTGGATTGGGCTCTGCCATATGCGCAGCGCTACTTACATAGTTTACATCCTGATAAATATTCTCAACTCAAGCAGTCTGGATTCGATATACTAAATCGTTTGCGAGTAGTAGAAGTTCAAAAGCTGTCCTACCGCAATGTTATAAAGATTGCTGGAAGTGAATCCAAGAAGCAAATCGAATGTAGTTGTGTTCTGCAGGATCACGATCTGTATACAACCCAGGAATCAGATTCTCACGCATTATTTATGGAGCTTTCTCGTTTGTTTTTTGATGGAAAACCAGAATTACACTTGGCAAATTTCCTTCATATGATCACAACAATGGCTGAATCAGGTTCAACTGAGGAGCAGATAGACTTTTTCATCTTCAATAGCCAAAAGGTGCCAAAGCTTCCTGATGGAGAATCTGTTTGGTCCCTGTCACATGTGCATAATGATAGGTTACCCCAGCCAAGTGACTGGCGCTTTGTTGGTTGGAAAGCCCCACTAAGCTTTGGTTATGCAAATCTTTTCAAGGCTCAGGCACAATTTGCACAGCTTACCAGTGTCTCACAAATTGAAATGGATAATGATTCTGAAACCCATGTCAGACAAACAGTTGAATCAACTCCCATTTCAGTCGACATTAAATGGACAATTGGTGAGGGTACAGCAACAACATCTGCACCTTTAGTGCTGCCAAACTCCAATGATTTGCAGGAACACTTTGATGATGAGACTGATATGGATACTGATTTCAATCCTAATAATCTAGATTTTGTTGTTGACCCTCGCGTTTTGGGTTCATCTGATTGTAGCAAGGTAGACCATCCTAGATATGGCTCTAGTGGAAGAGATTCAATGCGTGGAAGAGATGCAATGCTTACCGGGAGGTTAGGTGAGGTTGTTGCTTTTAAATACTTAATTGCAAAAGCTGGCAAGTCAGTTGTGAGGTGGGTTAATGAAAGAATTGAAACTGGGCTACCCTATGACATAGTTGTAGGTGAGAAGGAAGATAGTCTAGAGTTCATTGAGGTTAAGGCCACCCAACAACAAAAAAAGGATTGGTTCCACATATCAATGAGGGAGTGGCAATTTGCAGCGGAAAAAGGCGAAGCCTTCAGCATTTTGCACGTCCTCTTATTGGGTAATAATGCTGCAAGAGTCTCAGTTTACAAGAATCCTGTAAAATTGTGTCATCTGGGCAAGCTACAGTTGCATCTTTCGATGCCTAGGCAGGAGAAGGAATTATTTCTTGTGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

2847

Amino Acids

320.18

Weight (kDa)

6.12

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 1306 - 1406 2.5e-08 Sacsin domain
NOV_C PF13020 2726 - 2812 1.8e-19 Protein NO VEIN, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 7442
AasI GACNNNNNNGTC 2 cut(s) 488, 4505
AatII GACGTC 1 cut(s) 225
Acc16I TGCGCA 2 cut(s) 1457, 7323
Acc36I ACCTGC 2 cut(s) 3325, 5809
Acc65I GGTACC 1 cut(s) 852
AccB1I GGYRCC 5 cut(s) 382, 852, 1075, 2809, 7681
AccB7I CCANNNNNTGG 2 cut(s) 4904, 6099
AccI GTMKAC 6 cut(s) 5068, 6693, 6990, 7511, 7920, 8103
AccII CGCG 3 cut(s) 480, 1122, 8073
AccIII TCCGGA 5 cut(s) 2108, 2181, 2242, 5491, 6140
AcoI YGGCCR 2 cut(s) 3, 2749
AcvI CACGTG 1 cut(s) 1790
AcyI GRCGYC 3 cut(s) 222, 383, 5537
AdeI CACNNNGTG 1 cut(s) 4067
AfeI AGCGCT 1 cut(s) 7328
AflIII ACRYGT 5 cut(s) 1828, 2017, 3067, 4884, 7723
AhlI ACTAGT 2 cut(s) 766, 2672
AjiI CACGTC 2 cut(s) 1934, 8413
AjnI CCWGG 8 cut(s) 378, 515, 3303, 3774, 4772, 4801, 4861, 7518
AjuI GAANNNNNNNTTGG 2 cut(s) 2714, 2746
AleI CACNNNNGTG 2 cut(s) 1936, 6360
Alw21I GWGCWC 6 cut(s) 447, 2289, 3880, 4063, 5499, 6287
Alw44I GTGCAC 1 cut(s) 443
AlwNI CAGNNNCTG 1 cut(s) 2972
Ama87I CYCGRG 1 cut(s) 433
Aor13HI TCCGGA 5 cut(s) 2108, 2181, 2242, 5491, 6140
Aor51HI AGCGCT 1 cut(s) 7328
ApaI GGGCCC 1 cut(s) 5163
ApaLI GTGCAC 1 cut(s) 443
ArsI GACNNNNNNTTYG 6 cut(s) 1684, 1716, 1768, 1800, 7748, 7780
AseI ATTAAT 4 cut(s) 1209, 3753, 4943, 6554
Asp700I GAANNNNTTC 7 cut(s) 2546, 5004, 5331, 5454, 6017, 8134, 8529
Asp718I GGTACC 1 cut(s) 852
AspA2I CCTAGG 1 cut(s) 8510
AspLEI GCGC 7 cut(s) 385, 538, 1124, 1458, 7324, 7329, 7764
AsuC2I CCSGG 4 cut(s) 499, 2552, 6803, 8167
AsuHPI GGTGA 9 cut(s) 59, 596, 2746, 4415, 4736, 6099, 7952, 8189, 8294
AsuII TTCGAA 2 cut(s) 664, 952
AvaI CYCGRG 1 cut(s) 433
AvaII GGWCC 9 cut(s) 430, 746, 2500, 2848, 5020, 6061, 6721, 7268, 7713
AvrII CCTAGG 1 cut(s) 8510
AxyI CCTNAGG 3 cut(s) 426, 6411, 7216
BaeGI GKGCMC 4 cut(s) 55, 447, 2812, 5163
BaeI ACNNNNGTAYC 4 cut(s) 1164, 1197, 4224, 4257
BalI TGGCCA 1 cut(s) 2751
BamHI GGATCC 2 cut(s) 3005, 4094
BanI GGYRCC 5 cut(s) 382, 852, 1075, 2809, 7681
BanII GRGCYC 7 cut(s) 579, 2289, 3880, 5163, 5499, 6960, 7312
BauI CACGAG 3 cut(s) 446, 5597, 7248
BbrPI CACGTG 1 cut(s) 1790
BbsI GAAGAC 5 cut(s) 1329, 2366, 2598, 3263, 6590
Bbv12I GWGCWC 6 cut(s) 447, 2289, 3880, 4063, 5499, 6287
BbvCI CCTCAGC 3 cut(s) 1617, 5805, 6978
BceAI ACGGC 3 cut(s) 580, 1709, 5399
BcgI CGANNNNNNTGC 6 cut(s) 424, 458, 1671, 1705, 6347, 6381
BciT130I CCWGG 8 cut(s) 380, 517, 3305, 3776, 4774, 4803, 4863, 7520
BciVI GTATCC 2 cut(s) 4448, 8020
BclI TGATCA 2 cut(s) 2209, 7611
BcnI CCSGG 4 cut(s) 499, 2552, 6803, 8167
BcuI ACTAGT 2 cut(s) 766, 2672
BfoI RGCGCY 3 cut(s) 386, 7330, 7765
BfuAI ACCTGC 2 cut(s) 3325, 5809
BfuI GTATCC 2 cut(s) 4448, 8020
BglII AGATCT 1 cut(s) 4393
BlnI CCTAGG 1 cut(s) 8510
Bme18I GGWCC 9 cut(s) 430, 746, 2500, 2848, 5020, 6061, 6721, 7268, 7713
BmeT110I CYCGRG 1 cut(s) 433
BmgBI CACGTC 2 cut(s) 1934, 8413
BmrI ACTGGG 3 cut(s) 58, 6706, 8265
BmuI ACTGGG 3 cut(s) 58, 6706, 8265
BoxI GACNNNNGTC 1 cut(s) 6458
BpiI GAAGAC 5 cut(s) 1329, 2366, 2598, 3263, 6590
BpmI CTGGAG 4 cut(s) 16, 3758, 4146, 6500
Bpu10I CCTNAGC 5 cut(s) 1617, 3868, 5805, 6978, 7818
Bpu14I TTCGAA 2 cut(s) 664, 952
BpuEI CTTGAG 7 cut(s) 2460, 3894, 3950, 4277, 4415, 5591, 7352
BpuMI CCSGG 4 cut(s) 499, 2552, 6803, 8167
Bsa29I ATCGAT 1 cut(s) 4458
BsaAI YACGTR 1 cut(s) 1790
BsaBI GATNNNNATC 6 cut(s) 1818, 3552, 4398, 6351, 7503, 8037
BsaHI GRCGYC 3 cut(s) 222, 383, 5537
BsaI GGTCTC 2 cut(s) 3542, 7082
BsaWI WCCGGW 7 cut(s) 2108, 2181, 2242, 2383, 4440, 5491, 6140
Bse118I RCCGGY 1 cut(s) 5
Bse21I CCTNAGG 3 cut(s) 426, 6411, 7216
Bse3DI GCAATG 9 cut(s) 646, 1689, 2233, 5271, 5697, 6308, 7295, 7441, 8163
Bse8I GATNNNNATC 6 cut(s) 1818, 3552, 4398, 6351, 7503, 8037
BseAI TCCGGA 5 cut(s) 2108, 2181, 2242, 5491, 6140
BseBI CCWGG 8 cut(s) 380, 517, 3305, 3776, 4774, 4803, 4863, 7520
BseCI ATCGAT 1 cut(s) 4458
BseJI GATNNNNATC 6 cut(s) 1818, 3552, 4398, 6351, 7503, 8037
BseMI GCAATG 9 cut(s) 646, 1689, 2233, 5271, 5697, 6308, 7295, 7441, 8163
BseRI GAGGAG 8 cut(s) 54, 247, 842, 1078, 1343, 2138, 6843, 7658
BseSI GKGCMC 4 cut(s) 55, 447, 2812, 5163
BseYI CCCAGC 2 cut(s) 3707, 7746
BsgI GTGCAG 6 cut(s) 1032, 3711, 3866, 5397, 7212, 7947
Bsh1236I CGCG 3 cut(s) 480, 1122, 8073
Bsh1285I CGRYCG 1 cut(s) 460
BshNI GGYRCC 5 cut(s) 382, 852, 1075, 2809, 7681
BshVI ATCGAT 1 cut(s) 4458
BsiEI CGRYCG 1 cut(s) 460
BsiHKAI GWGCWC 6 cut(s) 447, 2289, 3880, 4063, 5499, 6287
BsiHKCI CYCGRG 1 cut(s) 433
BsmBI CGTCTC 5 cut(s) 514, 603, 1732, 3431, 4491
BsmI GAATGC 4 cut(s) 2716, 3410, 3989, 6094
Bso31I GGTCTC 2 cut(s) 3542, 7082
BsoBI CYCGRG 1 cut(s) 433
Bsp119I TTCGAA 2 cut(s) 664, 952
Bsp120I GGGCCC 1 cut(s) 5159
Bsp13I TCCGGA 5 cut(s) 2108, 2181, 2242, 5491, 6140
Bsp1407I TGTACA 2 cut(s) 3097, 7039
Bsp19I CCATGG 3 cut(s) 1243, 5526, 6079
Bsp68I TCGCGA 1 cut(s) 480
BspDI ATCGAT 1 cut(s) 4458
BspEI TCCGGA 5 cut(s) 2108, 2181, 2242, 5491, 6140
BspFNI CGCG 3 cut(s) 480, 1122, 8073
BspHI TCATGA 3 cut(s) 3436, 4476, 6811
BspMAI CTGCAG 3 cut(s) 3322, 6595, 7497
BspMI ACCTGC 2 cut(s) 3325, 5809
BspQI GCTCTTC 4 cut(s) 726, 1491, 3003, 6241
BspT104I TTCGAA 2 cut(s) 664, 952
BspT107I GGYRCC 5 cut(s) 382, 852, 1075, 2809, 7681
BspTNI GGTCTC 2 cut(s) 3542, 7082
BsrDI GCAATG 9 cut(s) 646, 1689, 2233, 5271, 5697, 6308, 7295, 7441, 8163
BsrFI RCCGGY 1 cut(s) 5
BsrGI TGTACA 2 cut(s) 3097, 7039
BssAI RCCGGY 1 cut(s) 5
BssNAI GTATAC 3 cut(s) 6694, 6991, 7512
BssNI GRCGYC 3 cut(s) 222, 383, 5537
BssSI CACGAG 3 cut(s) 446, 5597, 7248
Bst1107I GTATAC 3 cut(s) 6694, 6991, 7512
Bst2BI CACGAG 3 cut(s) 446, 5597, 7248
Bst2UI CCWGG 8 cut(s) 380, 517, 3305, 3776, 4774, 4803, 4863, 7520
BstACI GRCGYC 3 cut(s) 222, 383, 5537
BstAPI GCANNNNNTGC 5 cut(s) 5510, 6291, 6920, 7120, 7829
BstAUI TGTACA 2 cut(s) 3097, 7039
BstBAI YACGTR 1 cut(s) 1790
BstBI TTCGAA 2 cut(s) 664, 952
BstDSI CCRYGG 4 cut(s) 1243, 2885, 5526, 6079
BstEII GGTNACC 3 cut(s) 8, 2929, 7740
BstENI CCTNNNNNAGG 1 cut(s) 927
BstFNI CGCG 3 cut(s) 480, 1122, 8073
BstH2I RGCGCY 3 cut(s) 386, 7330, 7765
BstHHI GCGC 7 cut(s) 385, 538, 1124, 1458, 7324, 7329, 7764
BstMCI CGRYCG 1 cut(s) 460
BstNI CCWGG 8 cut(s) 380, 517, 3305, 3776, 4774, 4803, 4863, 7520
BstPAI GACNNNNGTC 1 cut(s) 6458
BstPI GGTNACC 3 cut(s) 8, 2929, 7740
BstSLI GKGCMC 4 cut(s) 55, 447, 2812, 5163
BstUI CGCG 3 cut(s) 480, 1122, 8073
BstV2I GAAGAC 5 cut(s) 1329, 2366, 2598, 3263, 6590
BstX2I RGATCY 9 cut(s) 1947, 2324, 3005, 4094, 4393, 6346, 6389, 6735, 6846
BstXI CCANNNNNNTGG 5 cut(s) 1250, 1938, 5573, 5975, 7758
BstYI RGATCY 9 cut(s) 1947, 2324, 3005, 4094, 4393, 6346, 6389, 6735, 6846
BstZ17I GTATAC 3 cut(s) 6694, 6991, 7512
Bsu15I ATCGAT 1 cut(s) 4458
Bsu36I CCTNAGG 3 cut(s) 426, 6411, 7216
BsuI GTATCC 2 cut(s) 4448, 8020
BsuTUI ATCGAT 1 cut(s) 4458
BtgI CCRYGG 4 cut(s) 1243, 2885, 5526, 6079
BtgZI GCGATG 2 cut(s) 6309, 7271
BtrI CACGTC 2 cut(s) 1934, 8413
BtsI GCAGTG 4 cut(s) 2422, 2625, 3030, 6375
BtuMI TCGCGA 1 cut(s) 480
BveI ACCTGC 2 cut(s) 3325, 5809
CaiI CAGNNNCTG 1 cut(s) 2972
CciI TCATGA 3 cut(s) 3436, 4476, 6811
CfoI GCGC 7 cut(s) 385, 538, 1124, 1458, 7324, 7329, 7764
Cfr10I RCCGGY 1 cut(s) 5
ClaI ATCGAT 1 cut(s) 4458
CseI GACGC 2 cut(s) 3413, 5545
CspCI CAANNNNNGTGG 4 cut(s) 753, 788, 1776, 1811
DinI GGCGCC 1 cut(s) 384
DraI TTTAAA 6 cut(s) 126, 2484, 2599, 5794, 7288, 8194
DraIII CACNNNGTG 1 cut(s) 4067
DrdI GACNNNNNNGTC 2 cut(s) 488, 4505
DseDI GACNNNNNNGTC 2 cut(s) 488, 4505
EaeI YGGCCR 2 cut(s) 3, 2749
EciI GGCGGA 2 cut(s) 14, 139
Ecl136II GAGCTC 3 cut(s) 2287, 3878, 5497
Eco24I GRGCYC 7 cut(s) 579, 2289, 3880, 5163, 5499, 6960, 7312
Eco31I GGTCTC 2 cut(s) 3542, 7082
Eco32I GATATC 2 cut(s) 819, 5899
Eco47I GGWCC 9 cut(s) 430, 746, 2500, 2848, 5020, 6061, 6721, 7268, 7713
Eco47III AGCGCT 1 cut(s) 7328
Eco53kI GAGCTC 3 cut(s) 2287, 3878, 5497
Eco72I CACGTG 1 cut(s) 1790
Eco81I CCTNAGG 3 cut(s) 426, 6411, 7216
Eco88I CYCGRG 1 cut(s) 433
Eco91I GGTNACC 3 cut(s) 8, 2929, 7740
EcoICRI GAGCTC 3 cut(s) 2287, 3878, 5497
EcoNI CCTNNNNNAGG 1 cut(s) 927
EcoO109I RGGNCCY 4 cut(s) 430, 746, 2848, 5020
EcoO65I GGTNACC 3 cut(s) 8, 2929, 7740
EcoRI GAATTC 4 cut(s) 406, 948, 3803, 6599
EcoRII CCWGG 8 cut(s) 378, 515, 3303, 3774, 4772, 4801, 4861, 7518
EcoRV GATATC 2 cut(s) 819, 5899
EcoT22I ATGCAT 4 cut(s) 640, 3410, 5506, 7125
EcoT38I GRGCYC 7 cut(s) 579, 2289, 3880, 5163, 5499, 6960, 7312
EgeI GGCGCC 1 cut(s) 384
EheI GGCGCC 1 cut(s) 384
Esp3I CGTCTC 5 cut(s) 514, 603, 1732, 3431, 4491
FalI AAGNNNNNCTT 8 cut(s) 1161, 1193, 1470, 1502, 2982, 3014, 6990, 7022
FauI CCCGC 2 cut(s) 244, 399
FauNDI CATATG 6 cut(s) 2065, 5371, 6631, 7125, 7318, 7608
FbaI TGATCA 2 cut(s) 2209, 7611
FblI GTMKAC 6 cut(s) 5068, 6693, 6990, 7511, 7920, 8103
FriOI GRGCYC 7 cut(s) 579, 2289, 3880, 5163, 5499, 6960, 7312
FspI TGCGCA 2 cut(s) 1457, 7323
GlaI GCGC 7 cut(s) 384, 537, 1123, 1457, 7323, 7328, 7763
GsaI CCCAGC 2 cut(s) 3711, 7750
GsuI CTGGAG 4 cut(s) 16, 3758, 4146, 6500
HaeII RGCGCY 3 cut(s) 386, 7330, 7765
HgaI GACGC 2 cut(s) 3413, 5545
HhaI GCGC 7 cut(s) 385, 538, 1124, 1458, 7324, 7329, 7764
Hin1I GRCGYC 3 cut(s) 222, 383, 5537
Hin6I GCGC 7 cut(s) 383, 536, 1122, 1456, 7322, 7327, 7762
HinP1I GCGC 7 cut(s) 383, 536, 1122, 1456, 7322, 7327, 7762
HincII GTYRAC 5 cut(s) 2295, 2743, 2905, 7921, 8065
HindII GTYRAC 5 cut(s) 2295, 2743, 2905, 7921, 8065
HindIII AAGCTT 6 cut(s) 941, 2270, 2478, 5766, 7688, 7789
HpaI GTTAAC 1 cut(s) 2743
HphI GGTGA 9 cut(s) 59, 596, 2746, 4415, 4736, 6099, 7952, 8189, 8294
Hpy99I CGWCG 2 cut(s) 485, 1938
HpyCH4IV ACGT 8 cut(s) 222, 507, 1009, 1769, 1789, 1933, 7103, 8412
HpySE526I ACGT 8 cut(s) 222, 507, 1009, 1769, 1789, 1933, 7103, 8412
Hsp92I GRCGYC 3 cut(s) 222, 383, 5537
HspAI GCGC 7 cut(s) 383, 536, 1122, 1456, 7322, 7327, 7762
KasI GGCGCC 1 cut(s) 382
Kpn2I TCCGGA 5 cut(s) 2108, 2181, 2242, 5491, 6140
KpnI GGTACC 1 cut(s) 856
Ksp22I TGATCA 2 cut(s) 2209, 7611
KspAI GTTAAC 1 cut(s) 2743
LguI GCTCTTC 4 cut(s) 726, 1491, 3003, 6241
LmnI GCTCC 8 cut(s) 1603, 1972, 4165, 5494, 5568, 6963, 7549, 7645
MaeII ACGT 8 cut(s) 222, 507, 1009, 1769, 1789, 1933, 7103, 8412
MfeI CAATTG 6 cut(s) 1548, 2087, 2790, 4608, 5541, 7935
MflI RGATCY 9 cut(s) 1947, 2324, 3005, 4094, 4393, 6346, 6389, 6735, 6846
MlsI TGGCCA 1 cut(s) 2751
MluNI TGGCCA 1 cut(s) 2751
Mly113I GGCGCC 1 cut(s) 383
Mox20I TGGCCA 1 cut(s) 2751
Mph1103I ATGCAT 4 cut(s) 640, 3410, 5506, 7125
MroI TCCGGA 5 cut(s) 2108, 2181, 2242, 5491, 6140
MroXI GAANNNNTTC 7 cut(s) 2546, 5004, 5331, 5454, 6017, 8134, 8529
MscI TGGCCA 1 cut(s) 2751
Msp20I TGGCCA 1 cut(s) 2751
MspA1I CMGCKG 2 cut(s) 4273, 8381
MteI GCGCNGCGC 1 cut(s) 7325
MunI CAATTG 6 cut(s) 1548, 2087, 2790, 4608, 5541, 7935
Mva1269I GAATGC 4 cut(s) 2716, 3410, 3989, 6094
MvaI CCWGG 8 cut(s) 380, 517, 3305, 3776, 4774, 4803, 4863, 7520
MvnI CGCG 3 cut(s) 480, 1122, 8073
NarI GGCGCC 1 cut(s) 383
NciI CCSGG 4 cut(s) 499, 2552, 6803, 8167
NcoI CCATGG 3 cut(s) 1243, 5526, 6079
NdeI CATATG 6 cut(s) 2065, 5371, 6631, 7125, 7318, 7608
NmeAIII GCCGAG 2 cut(s) 491, 586
NruI TCGCGA 1 cut(s) 480
NsbI TGCGCA 2 cut(s) 1457, 7323
NsiI ATGCAT 4 cut(s) 640, 3410, 5506, 7125
NspV TTCGAA 2 cut(s) 664, 952
OliI CACNNNNGTG 2 cut(s) 1936, 6360
PaeI GCATGC 4 cut(s) 977, 2446, 3383, 3863
PagI TCATGA 3 cut(s) 3436, 4476, 6811
PciI ACATGT 5 cut(s) 1828, 2017, 3067, 4884, 7723
PciSI GCTCTTC 4 cut(s) 726, 1491, 3003, 6241
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PctI GAATGC 4 cut(s) 2716, 3410, 3989, 6094
PdmI GAANNNNTTC 7 cut(s) 2546, 5004, 5331, 5454, 6017, 8134, 8529
PflFI GACNNNGTC 1 cut(s) 5549
PflMI CCANNNNNTGG 2 cut(s) 4904, 6099
PluTI GGCGCC 1 cut(s) 386
PmaCI CACGTG 1 cut(s) 1790
PmlI CACGTG 1 cut(s) 1790
Ppu21I YACGTR 1 cut(s) 1790
PpuMI RGGWCCY 4 cut(s) 430, 746, 2848, 5020
PscI ACATGT 5 cut(s) 1828, 2017, 3067, 4884, 7723
PshAI GACNNNNGTC 1 cut(s) 6458
PshBI ATTAAT 4 cut(s) 1209, 3753, 4943, 6554
PsiI TTATAA 1 cut(s) 7442
Psp124BI GAGCTC 3 cut(s) 2289, 3880, 5499
Psp5II RGGWCCY 4 cut(s) 430, 746, 2848, 5020
Psp6I CCWGG 8 cut(s) 378, 515, 3303, 3774, 4772, 4801, 4861, 7518
PspCI CACGTG 1 cut(s) 1790
PspEI GGTNACC 3 cut(s) 8, 2929, 7740
PspFI CCCAGC 2 cut(s) 3707, 7746
PspGI CCWGG 8 cut(s) 378, 515, 3303, 3774, 4772, 4801, 4861, 7518
PspOMI GGGCCC 1 cut(s) 5159
PspPPI RGGWCCY 4 cut(s) 430, 746, 2848, 5020
PsrI GAACNNNNNNTAC 2 cut(s) 7398, 7430
PstI CTGCAG 3 cut(s) 3322, 6595, 7497
PstNI CAGNNNCTG 1 cut(s) 2972
PsuI RGATCY 9 cut(s) 1947, 2324, 3005, 4094, 4393, 6346, 6389, 6735, 6846
PsyI GACNNNGTC 1 cut(s) 5549
PvuII CAGCTG 1 cut(s) 4273
RruI TCGCGA 1 cut(s) 480
SacI GAGCTC 3 cut(s) 2289, 3880, 5499
SalI GTCGAC 1 cut(s) 7919
SapI GCTCTTC 4 cut(s) 726, 1491, 3003, 6241
SfoI GGCGCC 1 cut(s) 384
SfuI TTCGAA 2 cut(s) 664, 952
SinI GGWCC 9 cut(s) 430, 746, 2500, 2848, 5020, 6061, 6721, 7268, 7713
SmlI CTYRAG 7 cut(s) 2475, 3909, 3929, 4256, 4430, 5606, 7367
SmoI CTYRAG 7 cut(s) 2475, 3909, 3929, 4256, 4430, 5606, 7367
SpeI ACTAGT 2 cut(s) 766, 2672
SphI GCATGC 4 cut(s) 977, 2446, 3383, 3863
SspDI GGCGCC 1 cut(s) 382
SspI AATATT 4 cut(s) 779, 957, 3751, 7358
SstI GAGCTC 3 cut(s) 2289, 3880, 5499
TaiI ACGT 8 cut(s) 225, 510, 1012, 1772, 1792, 1936, 7106, 8415
TatI WGTACW 6 cut(s) 2644, 3097, 6838, 7022, 7039, 7187
TauI GCSGC 3 cut(s) 181, 497, 1105
TspGWI ACGGA 4 cut(s) 1998, 4716, 6501, 7189
Tth111I GACNNNGTC 1 cut(s) 5549
Van91I CCANNNNNTGG 2 cut(s) 4904, 6099
VneI GTGCAC 1 cut(s) 443
VpaK11BI GGWCC 9 cut(s) 430, 746, 2500, 2848, 5020, 6061, 6721, 7268, 7713
VspI ATTAAT 4 cut(s) 1209, 3753, 4943, 6554
XagI CCTNNNNNAGG 1 cut(s) 927
XbaI TCTAGA 3 cut(s) 3133, 8050, 8299
XcmI CCANNNNNNNNNTGG 3 cut(s) 1250, 4753, 8114
XmaJI CCTAGG 1 cut(s) 8510
XmiI GTMKAC 6 cut(s) 5068, 6693, 6990, 7511, 7920, 8103
XmnI GAANNNNTTC 7 cut(s) 2546, 5004, 5331, 5454, 6017, 8134, 8529
ZraI GACGTC 1 cut(s) 223
Zsp2I ATGCAT 4 cut(s) 640, 3410, 5506, 7125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.