Rh6BG207000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000582
Physical Location & Seq
Forward (+)
39008191 .. 39024872
16682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG207000.1

Sequence Viewer

Length: 6465 bp
ATGTACGGACGTCCTTCTCGCTCCTCCGGCGGCACATGGGGTCAGCCACCGCCGCTTGCACAGCCTCTAAACTCTAATTTCTCACTCCAAAACCCCAACACTCCGTACATCTACCCTATAAACCCTGCCTTTACGCCCCACCATGCCTACCGAAACTTCTCCCCCAACAATCTCCCACCGCAGAACCCTAGCTTCGTTCCCCAGCAGTTCTCGAATCCGCCTTTTCGGCCTCCGCCGCCTCAGGGTAACTCCAAAGAGCTGCTGGAGAAAGTCGACCGCGCCGTCGCGAAGGCTCGGTCGGACCTTGTTGCCGCCGGCGACAGCGTCTCGGCGTGGAAGGTCTCGCGGTCGGCGCTTCTGATGCTACAGATTGATGGCTGGAGCTCTCTGGGGTTTCAGATGCAGCAGGTGCCGTCTCTACACCGCCTCATGTTCACTGAAGGCAAGATAAATGCATTCATTCAGTGTTTTGTTGCGGTTCGAAGAATTTCTTCATTATACGATCTGGAAGTAGCAGTCTGCAAGAATGAGGGCATCGAGAAGTTTGAAGAGCTAGGGTTGGGCCCTTTGGTGCGACACCCACTTGTTTTGCATTATTACTCAGTGAAGCCTGATACTAATGAAGTTTATAAGATTACTAGCGATGAGATAATATCTTTACTCTCTGAGTACATGGATACCTGCAAGAATAAAGAAATTAAGATTGAAGAATTTTTAGATTTCATTGTAAAGAAGCGATTTGTTGCAAGCAAGGAAGATCTTGGAATACGAATTCAGAGCATGGGGATGCATATTTCTGCTATCCGGGATGTGAAGAAAACTGAACAGGTTCTTAAGCAAACATCTAAGAAAGGTAACAATAAGCGTTACTTCTCATTGAAAAGGCAGCTAGACGAACGTTTCAACGACATTTCTCAGCGTGTTGAATCATTTTCCTCCGTACAGAAGTTTTGTGGCAAGCACATTAGGTTTGATTCTTCAAAAGACTCGGAGGCTGACAGTAGTGATGATAATTCATATGAAGATGATGATGAAGTTAATGACCATTCGACAGGCAATCAGGTTAACTTATTATCAAAATCTGTTACAACTTCTGATCGAGCTAGCAGATGTCCTTACCCTTCTGAATTAGAAGAGAAGAAACGGCTAGGATTGAACCAGCATTCCCCAGCTAGTTGCTCCCAAAAGCTTGGTGAGAGTAATCAGTCAGCTAAAAAGAAAAGAAACTCTGAAGATGTAAACAGTCCAATCTCAGTGCCCACCAAATTGCGTAAGAGAGATAAGACGGGAGAAGATGCTCCTCGAACAGGGCATGGCTGGAAAACCAATGAGGTTAACAATTTGGATGAAAATGATCTCTCAATTACTGATAGCTGTTTAAAGACCTTCGTTACAACTTGGAAGGAGGCATGTCGGCAGAAAACTGTGGCTGAGGTACTTGATAGATTACTGCAGCTTAATAACATAGAAGCTCAGAAGAAAAAGGAAATCAAGTCAATGTTTTCTTTGGATCCATTAATTGGATTGTTAAATGTTGCTGTCTCATCTATCAAATCCGGATTGTGGGATAGCATGTATGATACTTTCCAAACTGTTGGTCAGTTAACTGACAGTCTTCCGGATAACTGTCCTGAATATGTGAACATAGATGTTGAACCAAGTATTAAAGATGTGAAAAGTACCAAGGATGCACCAGTCATTCCTGAACACGCTGTAGACCAGAGTGTTCCTGTTGAGGACATAATCAGGAAACTTACAATGTATTTTGAGATTGATCGAGGTGTACATGGCAATGGTAAATCACTTCAGGAGAAAATATGTACCTTCTTGACAAAGCTTTACAGTTGTGAACTGTGGTTAGTGGAAGAATTTTCTGTCAAGGAATTTAGATCGCTTGGTCATGGAGAGTTTTTAAGGTTCTTAGAAAATTATGCTGGTCTACTTCCACAAGACCTGTGCAAGTTCCTGACTGATGATGTAATTGAGAAGTGCCCTTTGGAAGTTTGCATGCTCCAGCACCATTTGGTTGTTTTACTATCTCAAGCTTTAAATAGTTTATGGGAGGATGAAAATATCACAAAACAGGACATTCTGTTGCTGCTTAGGAAGCAATTCCCATTGGTTAGTTTCAAAACTATTGAAAATGGTTCAGTAGAAGATTATTTAAGCATAGTAGGAAAGCATAAAAATGCTGTGATTTCCAAATGTGTTCTGTTCTCAATGGCATTACATGGCACTAGCTATGCGATAGACTCATTAGTACATTATGAGAACGTCCTATTGAAAAGCATGTCATTGAGTTCTGACAGTGGCCAGAAAGCAAAAACCCAGGTTACATCCAAGGATGCAATTAAAGTCTTAGCTAGGGCACCAATGATGTCTGACTTAAACTTATGGTCACACTGGGACCTTTTGTTTGCTCCTTCACTTGGTCCTCTTGTACCATGGTTATTAAATGAAGTTAACACAGATGAATTGTTGTGTTTGGTGACCAAAGATGGAAAAGTCTTTCGGTTAGACCAATCAGCTACTGTAGATTCCTTCGTGGAGGCTGCCCTTCAAGGATCCTCTTTCCAAACAGCAGTGAAATTGTTATCTCTGTTTTCACTAGTTGGGGGAGAAAAACATGTCCCTGTGCCTCTTTTGAAAATCCATATGCAACATGCATTTGAAGTCATTTTGAAAAATTTTGTAGATGACATGGTAGTACATGACAATAAGAATTCTCTTAATTATGGAAAAGCGTTGTCTGGACAACAAATGATTGGTGAAATTGCAGCTGGTAAACTATCACACAGAGACTTACACAAGACAGATATTGGAAAACCCATTATATCAAGATTTGTCCTTGAATGTCTTGGGTATTTACCTGCAGAATTTCGTGGTTTTGCTGCTGATGTATTGCTTTCGGGAATGCAGTCTGTTGTCAAACATGCTGCTTCGGCCATATTGAGTGAGTGCAGCCAATCAGAGCAACGTATCATGCTTCATGAAGTTGGTTTGTCTCTTGGTATAGTTGAGTGGATTAATGATTACTACACATTTCTTTCCAATGGTACGGGTCAGTCGTTCATATCTGGGGCCTCATGTTCCAATGCTGTTGGATATGAAATGGGGTCAGGTTCAAAGCCCTTGCAAGATGTTTCAGATAAGTTTTTGACTTCTGGAGGGAGCATGGCTGGATCTGTTGTGGAAGATGTGCAGGAAGTTGGATGTACTGATGTCTCTCTGAAAATTGGTGGTGCGGAAATCTCTGACGAGAGAGCTGGCTGTGGTTACACACAACATTCTGCCAAAATTAGTGAATATGAAGATGCATCCCAGGTGATTGAGTCAATCAGAAGAGATGAATTTGGTCTTCATTCAAGCCAAACGACCTCTGAAAGTATCATGTTGAAGAAGCATCATGCTCGCTTAGGGAGAGCTCTACATTGTCTTTCACAGGAATTGTATTCTCAGGATTCACATTTTCTTCTTGAGCTGGTTCAAAATGCAGATGATAATACATACCCAACATATGTAGAACCAACTTTAACATTCATTCTTCAAGACTCGGGTATTATTGTTTTAAATAATGAGCAAGGCTTCTCTGCCGAGAACATTAGAGCACTTTGTGATGTTGGAAGTTCAACCAAGAAAGGATCTAATGCTGGCTATATTGGGCGGAAGGGCATTGGTTTCAAATCTGTGTTTCGGGTCACTGATGCTCCAGAAATTCATTCTAATGGATTCCATATCAAGTTTGATATAAGTCAGGGTCAGATTGGTTTTCTTCTGCCAACAGTAGTACCTCCTTGCAACATTGACATGTTAAGTAGGCTGACTTCTAGTGACAGTGATCAACAGGACAGTAACTTCTGGAACACTTGTATTGTTCTTCCTTTCAGATCGAAGTTTTCAGATGGATCTGTCATGAAAAGCATTATAAATATGTTTTCAGATCTTCATCCATCTTTATTACTCTTCCTTCACCGCCTCCAATGTATAAAGTTTAGAAACTTGCTTGATAATTCACTGACTGTCATGAGAAAAGAAACTGTGGGAGATGGTATAGTAAAGGTTTCACATGGGAAAGAGAAAATGACATGGTTCTTAGTATCTCAGAAATTGCAAGCGGATTTTATACGTAGTGATGTACAAACAACAGAAATCTCCATAGCATTTACATTGAAGGAATTAGATAATGGTGATTATGGACCAGATCTAGGGCAACAGCCTGCTTTTGCATTTCTTCCTCTAAGAACCTATGGCCTGAAGTTTATTGTTCAGGGTGATTTTGTTCTTCCTTCATCTAGAGAGGAAGTGGATGGAGATAGTCCCTGGAACCAGTGGCTATTATCAGAATTTCCTGGTTTATTTGTCAATGCTGAGAGGTCTTTTTGTTCTCTTCCCTGCTTTAGAGAGAACCCCGGGAGAGCAGTGGCAGCTTATATGTCATTTGTTCCACTTGTTGGGGAAGTGCATGGGTTTTTTTCTTCTCTTCCTCGATTAATCATTTCTAAATTACGGATGTCAAATTGCTTACTCCTGGAAGGAGGAAATAACGAGTGGGTTCCTCCTTGCAAGGTCCTAAGAGGTTGGAATGAGCAGGCCCGATTGCTTCTGCCTGATAGTTTACTTCGGGAGCATCTTGGCCTTGGGTTGTTGGATAAAAATATAGTTCTGCCTGATTCACTTGCAAGAGCACTCGGCATTGCAGACTATGGACCCAAAGTTCTGCTTCAAGTTATGGACTCCTTATGTCGTTTGCAAAATGGCCTCACATCAATGGGACTGGGTTGGTTGACCTCTTGGCTAAGTGAACTGTATGCAATGTCATTCAATGCTTCTGTGGAAACATCATTTGACTCTGGACTTGGAATGGATTTGATAGAAGAACTCCGAAAAATTCCATTCATTCCTCTTTCAGATGGTACATATGGTGCAGTAGACAAAGACCCAATTTGGTTACATTTTGATGCCTTGAGCACTGGGTTTGAGGGTCAGCATGGACTTGAGTCTTTTCCTAACTTGTATGCTAATCTTCGTATCGTAAGCCCTGCCTTCCTTTCGACATCATGTGTAGATATGTCATCCATGGATGTGACTACAATTGACAAACAAATACGTATGCTTCGTAGAATTGGTGTCCAGCAGTTGTCCGCGCATGAAATTGTCAAGGTACACATCTTGCCAGCTATATCTGATAACAGAAATGCAGCTAGGGATAAGAATTTGATGACCGAATATCTCTGCTTTGTAATGGTTCACCTACAGTCGACCTGCTCCGATTGTCATGGTGAAAAGGAATACATAATGTCAGAATTACGAAATAAAGCTTATGTACTAACAAATCATGGCTTTAAACGAGCTGCTGAGATATCTATTCATTTCAGTAAGGAATATGGAAATCCTATCGACATAAATAAGTTGATAAATATGGTGGATATGAAGTGGCATGAGGTTGACATCTCCTATTTGAAGCATCCAGTTACAAAGTCACTCCAATGTGAACTAACGAAGTGGAGGGAATTTTTCCAGCAAATTGGCATCGTGGATTTCGTGAAAGTAGTTCACATTGAAAAAGGATTTAATGATACATGTAAGGACTTGGTTTCTCTTGGATCAAATGTCACAGATTGGGAGTCCCCTGAATTGGTCAGTTTATTGTCCCTGTTGACCAGAACTGGCAACAAAAAAGGCTGTGAATATCTTTTACAGGTGCTTGATAGTTTATGGGATGAATGCTATCTTGAAAAAGCTACAGGCTACTGCGCTTCTAAAGATGTAGTGGAGAAAAAGCCTTTTAAATCTTCATTTATAAGCAGCATCTGTGATGCACAATGGGTAGTATCAACGATGGATGATGCACTTCACTATCCTAGAGATTTATACCATGACTGTGATGCAGTGCGCTCGGTTCTTGGTCCATCTGCTCCATTTTCTGTTCCAAAGATAGGGAGTGGAAATTTTGCAAGTGCTATGGGGTTCAAGACATTAGTTTCTGTTGACGATGGTCTAGAGGTATTGAAACTATGGAGGTGTGATAACCCATTCAGGGCCAGCATAGCGCAAATGTTCAAATTTTACACATTAATCTGGAACGAAATGGCCACTTCGAAGCCAAGAGTTTTAGAGGAGTTCCATTCCAAACCTTCTATTTTTGTTCCATATGCATCTAGCTCAAGGCATGAGGATGTGGTGTCTGGTACATTTTTGTCCCCTGAGGAAGTATATTGGTATGATTCGACTTCCTTCGTGGACCAAATCAAGCAGATACATCCTCAGTGCAGCCCAACAGGTGTTGCTCATGGCCCCTTGAATAAGACATTGTGCAACTTTTATCCTGGCCTTCATGACTTCTTTGTTGATGGATGTGGAGTACCTGAGACCCCTCCTCTCCGTAGCTACCTACAGATTTTGCTGCATTTATCAAAGGTTGCTCTGCCTTCACAAGCAGCTAATGCCGTAAGTAATCAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

2154

Amino Acids

240.75

Weight (kDa)

5.84

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 1151 - 1336 2.5e-09 Sacsin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 630, 3926, 5801
AarI CACCTGC 1 cut(s) 397
AasI GACNNNNNNGTC 1 cut(s) 281
AatII GACGTC 1 cut(s) 13
Acc36I ACCTGC 4 cut(s) 397, 689, 2878, 5270
AccB1I GGYRCC 2 cut(s) 409, 2368
AccB7I CCANNNNNTGG 2 cut(s) 1520, 4451
AccI GTMKAC 5 cut(s) 273, 1716, 1939, 4929, 5258
AccII CGCG 4 cut(s) 279, 287, 346, 5144
AccIII TCCGGA 2 cut(s) 1556, 1618
AclI AACGTT 1 cut(s) 898
AclWI GGATC 8 cut(s) 1505, 1518, 2559, 2572, 3190, 3649, 3913, 5611
AcoI YGGCCR 3 cut(s) 2311, 2943, 6090
AcuI CTGAAG 4 cut(s) 459, 1251, 1790, 4274
AcyI GRCGYC 1 cut(s) 10
AdeI CACNNNGTG 1 cut(s) 3614
AflII CTTAAG 1 cut(s) 833
AflIII ACRYGT 3 cut(s) 2626, 3807, 5579
AhlI ACTAGT 1 cut(s) 2608
AjnI CCWGG 6 cut(s) 2328, 3321, 4319, 4348, 4527, 6325
AjuI GAANNNNNNNTTGG 2 cut(s) 1979, 2011
AloI GAACNNNNNNTCC 4 cut(s) 1148, 1180, 4698, 4730
Alw21I GWGCWC 5 cut(s) 386, 3427, 3610, 4687, 4970
Alw26I GTCTC 8 cut(s) 331, 346, 420, 1546, 2793, 3009, 3229, 6362
AlwI GGATC 8 cut(s) 1505, 1518, 2559, 2572, 3190, 3649, 3913, 5611
AlwNI CAGNNNCTG 2 cut(s) 2531, 5811
Ama87I CYCGRG 2 cut(s) 3553, 4409
Aor13HI TCCGGA 2 cut(s) 1556, 1618
ApaI GGGCCC 1 cut(s) 566
ArsI GACNNNNNNTTYG 6 cut(s) 281, 313, 1582, 1614, 2399, 2431
AseI ATTAAT 4 cut(s) 1517, 3027, 4490, 6074
Asp700I GAANNNNTTC 4 cut(s) 487, 490, 828, 2111
AspLEI GCGC 6 cut(s) 281, 355, 5146, 5756, 5895, 6052
AsuC2I CCSGG 3 cut(s) 806, 4410, 4411
AsuHPI GGTGA 9 cut(s) 1205, 2500, 2780, 3337, 3962, 4199, 4283, 5240, 5291
AsuII TTCGAA 2 cut(s) 481, 6098
AsuNHI GCTAGC 1 cut(s) 1103
AvaI CYCGRG 2 cut(s) 3553, 4409
AvaII GGWCC 8 cut(s) 301, 2407, 2432, 4196, 4567, 4706, 5906, 6241
AxyI CCTNAGG 2 cut(s) 240, 6204
BaeGI GKGCMC 4 cut(s) 566, 1260, 1994, 2371
BaeI ACNNNNGTAYC 2 cut(s) 3771, 3804
BalI TGGCCA 2 cut(s) 2313, 6092
BamHI GGATCC 2 cut(s) 1510, 2564
BanI GGYRCC 2 cut(s) 409, 2368
BanII GRGCYC 3 cut(s) 386, 566, 3427
BarI GAAGNNNNNNTAC 2 cut(s) 3808, 3840
BbsI GAAGAC 2 cut(s) 1607, 3350
Bbv12I GWGCWC 5 cut(s) 386, 3427, 3610, 4687, 4970
BbvCI CCTCAGC 1 cut(s) 1431
BceAI ACGGC 4 cut(s) 266, 397, 1160, 6431
BcgI CGANNNNNNTGC 4 cut(s) 3392, 3426, 5877, 5911
BciT130I CCWGG 6 cut(s) 2330, 3323, 4321, 4350, 4529, 6327
BciVI GTATCC 1 cut(s) 670
BclI TGATCA 1 cut(s) 3838
BcnI CCSGG 3 cut(s) 806, 4410, 4411
BcoDI GTCTC 8 cut(s) 331, 346, 420, 1546, 2793, 3009, 3229, 6362
BcuI ACTAGT 1 cut(s) 2608
BfmI CTRYAG 8 cut(s) 365, 1451, 1713, 2532, 2871, 5252, 5742, 6392
BfoI RGCGCY 1 cut(s) 356
BfrI CTTAAG 1 cut(s) 833
BfuAI ACCTGC 4 cut(s) 397, 689, 2878, 5270
BfuI GTATCC 1 cut(s) 670
BglI GCCNNNNNGGC 1 cut(s) 226
BglII AGATCT 3 cut(s) 757, 3940, 4201
Bme1390I CCNGG 9 cut(s) 806, 2330, 3323, 4321, 4350, 4410, 4411, 4529, 6327
Bme18I GGWCC 8 cut(s) 301, 2407, 2432, 4196, 4567, 4706, 5906, 6241
BmeT110I CYCGRG 2 cut(s) 3553, 4409
BmrFI CCNGG 9 cut(s) 806, 2330, 3323, 4321, 4350, 4410, 4411, 4529, 6327
BmrI ACTGGG 3 cut(s) 2413, 4784, 4980
BmtI GCTAGC 1 cut(s) 1107
BmuI ACTGGG 3 cut(s) 2413, 4784, 4980
BoxI GACNNNNGTC 2 cut(s) 4996, 5994
BpiI GAAGAC 2 cut(s) 1607, 3350
BpmI CTGGAG 5 cut(s) 284, 400, 1997, 3186, 3693
Bpu10I CCTNAGC 3 cut(s) 1431, 2102, 3415
Bpu14I TTCGAA 2 cut(s) 481, 6098
BpuEI CTTGAG 5 cut(s) 2025, 3497, 4984, 5015, 6148
BpuMI CCSGG 3 cut(s) 806, 4410, 4411
BsaAI YACGTR 2 cut(s) 4127, 5108
BsaBI GATNNNNATC 1 cut(s) 3945
BsaHI GRCGYC 1 cut(s) 10
BsaI GGTCTC 2 cut(s) 346, 6362
BsaWI WCCGGW 2 cut(s) 1556, 1618
BsaXI ACNNNNNCTCC 2 cut(s) 3691, 3721
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 7 cut(s) 1694, 2408, 4327, 4779, 4975, 5468, 5671
Bse21I CCTNAGG 2 cut(s) 240, 6204
Bse3DI GCAATG 3 cut(s) 1798, 4692, 4818
Bse8I GATNNNNATC 1 cut(s) 3945
BseAI TCCGGA 2 cut(s) 1556, 1618
BseBI CCWGG 6 cut(s) 2330, 3323, 4321, 4350, 4529, 6327
BseJI GATNNNNATC 1 cut(s) 3945
BseMI GCAATG 3 cut(s) 1798, 4692, 4818
BseNI ACTGG 7 cut(s) 1694, 2408, 4327, 4779, 4975, 5468, 5671
BseRI GAGGAG 4 cut(s) 13, 1290, 6131, 6366
BseSI GKGCMC 4 cut(s) 566, 1260, 1994, 2371
BseYI CCCAGC 2 cut(s) 201, 1168
BsgI GTGCAG 4 cut(s) 2980, 3221, 4944, 6289
Bsh1236I CGCG 4 cut(s) 279, 287, 346, 5144
Bsh1285I CGRYCG 3 cut(s) 277, 299, 350
BshNI GGYRCC 2 cut(s) 409, 2368
BsiEI CGRYCG 3 cut(s) 277, 299, 350
BsiHKAI GWGCWC 5 cut(s) 386, 3427, 3610, 4687, 4970
BsiHKCI CYCGRG 2 cut(s) 3553, 4409
BsiSI CCGG 6 cut(s) 27, 315, 805, 1557, 1619, 4410
BslFI GGGAC 7 cut(s) 2420, 2615, 4302, 4785, 5611, 5635, 6184
BsmAI GTCTC 8 cut(s) 331, 346, 420, 1546, 2793, 3009, 3229, 6362
BsmBI CGTCTC 2 cut(s) 331, 420
BsmFI GGGAC 7 cut(s) 2420, 2615, 4302, 4785, 5611, 5635, 6184
BsmI GAATGC 4 cut(s) 455, 1162, 2919, 5729
Bso31I GGTCTC 2 cut(s) 346, 6362
BsoBI CYCGRG 2 cut(s) 3553, 4409
Bsp119I TTCGAA 2 cut(s) 481, 6098
Bsp120I GGGCCC 1 cut(s) 562
Bsp1286I GDGCHC 9 cut(s) 386, 566, 1260, 1994, 2371, 3427, 3610, 4687, 4970
Bsp13I TCCGGA 2 cut(s) 1556, 1618
Bsp1407I TGTACA 2 cut(s) 1783, 4135
Bsp19I CCATGG 2 cut(s) 2444, 5076
Bsp68I TCGCGA 1 cut(s) 287
BspEI TCCGGA 2 cut(s) 1556, 1618
BspFNI CGCG 4 cut(s) 279, 287, 346, 5144
BspHI TCATGA 4 cut(s) 2989, 3912, 4023, 6334
BspMAI CTGCAG 2 cut(s) 1455, 2875
BspMI ACCTGC 4 cut(s) 397, 689, 2878, 5270
BspOI GCTAGC 1 cut(s) 1107
BspPI GGATC 8 cut(s) 1505, 1518, 2559, 2572, 3190, 3649, 3913, 5611
BspQI GCTCTTC 1 cut(s) 543
BspT104I TTCGAA 2 cut(s) 481, 6098
BspT107I GGYRCC 2 cut(s) 409, 2368
BspTI CTTAAG 1 cut(s) 833
BspTNI GGTCTC 2 cut(s) 346, 6362
BsrDI GCAATG 3 cut(s) 1798, 4692, 4818
BsrFI RCCGGY 1 cut(s) 314
BsrGI TGTACA 2 cut(s) 1783, 4135
BsrI ACTGG 7 cut(s) 1694, 2408, 4327, 4779, 4975, 5468, 5671
BssAI RCCGGY 1 cut(s) 314
BssNI GRCGYC 1 cut(s) 10
BssT1I CCWWGG 5 cut(s) 1683, 2340, 2444, 4636, 5076
Bst2UI CCWGG 6 cut(s) 2330, 3323, 4321, 4350, 4529, 6327
Bst6I CTCTTC 6 cut(s) 543, 1128, 3337, 3968, 4392, 4485
BstACI GRCGYC 1 cut(s) 10
BstAFI CTTAAG 1 cut(s) 833
BstAPI GCANNNNNTGC 2 cut(s) 409, 6443
BstAUI TGTACA 2 cut(s) 1783, 4135
BstBAI YACGTR 2 cut(s) 4127, 5108
BstBI TTCGAA 2 cut(s) 481, 6098
BstDSI CCRYGG 2 cut(s) 2444, 5076
BstEII GGTNACC 1 cut(s) 2488
BstENI CCTNNNNNAGG 1 cut(s) 1305
BstFNI CGCG 4 cut(s) 279, 287, 346, 5144
BstH2I RGCGCY 1 cut(s) 356
BstHHI GCGC 6 cut(s) 281, 355, 5146, 5756, 5895, 6052
BstMAI GTCTC 8 cut(s) 331, 346, 420, 1546, 2793, 3009, 3229, 6362
BstMCI CGRYCG 3 cut(s) 277, 299, 350
BstNI CCWGG 6 cut(s) 2330, 3323, 4321, 4350, 4529, 6327
BstNSI RCATGY 9 cut(s) 1413, 1576, 2011, 2293, 2630, 2666, 2936, 3811, 5583
BstPAI GACNNNNGTC 2 cut(s) 4996, 5994
BstPI GGTNACC 1 cut(s) 2488
BstSCI CCNGG 9 cut(s) 804, 2328, 3321, 4319, 4348, 4408, 4409, 4527, 6325
BstSFI CTRYAG 8 cut(s) 365, 1451, 1713, 2532, 2871, 5252, 5742, 6392
BstSLI GKGCMC 4 cut(s) 566, 1260, 1994, 2371
BstSNI TACGTA 2 cut(s) 4127, 5108
BstUI CGCG 4 cut(s) 279, 287, 346, 5144
BstV2I GAAGAC 2 cut(s) 1607, 3350
BstX2I RGATCY 8 cut(s) 757, 1510, 2564, 3182, 3641, 3905, 3940, 4201
BstXI CCANNNNNNTGG 3 cut(s) 1190, 1595, 5525
BstYI RGATCY 8 cut(s) 757, 1510, 2564, 3182, 3641, 3905, 3940, 4201
Bsu36I CCTNAGG 2 cut(s) 240, 6204
BsuI GTATCC 1 cut(s) 670
BtgI CCRYGG 2 cut(s) 2444, 5076
BtgZI GCGATG 1 cut(s) 657
BtsI GCAGTG 3 cut(s) 2589, 4425, 5895
BtuMI TCGCGA 1 cut(s) 287
BveI ACCTGC 4 cut(s) 397, 689, 2878, 5270
CaiI CAGNNNCTG 2 cut(s) 2531, 5811
CciI TCATGA 4 cut(s) 2989, 3912, 4023, 6334
CfoI GCGC 6 cut(s) 281, 355, 5146, 5756, 5895, 6052
Cfr10I RCCGGY 1 cut(s) 314
Cfr9I CCCGGG 1 cut(s) 4409
CseI GACGC 1 cut(s) 313
CspCI CAANNNNNGTGG 2 cut(s) 570, 605
DraI TTTAAA 5 cut(s) 1380, 2049, 3570, 5344, 5788
DraIII CACNNNGTG 1 cut(s) 3614
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
EaeI YGGCCR 3 cut(s) 2311, 2943, 6090
Eam1104I CTCTTC 6 cut(s) 543, 1128, 3337, 3968, 4392, 4485
EarI CTCTTC 6 cut(s) 543, 1128, 3337, 3968, 4392, 4485
EciI GGCGGA 3 cut(s) 207, 222, 3679
Ecl136II GAGCTC 2 cut(s) 384, 3425
Eco105I TACGTA 2 cut(s) 4127, 5108
Eco130I CCWWGG 5 cut(s) 1683, 2340, 2444, 4636, 5076
Eco24I GRGCYC 3 cut(s) 386, 566, 3427
Eco31I GGTCTC 2 cut(s) 346, 6362
Eco32I GATATC 1 cut(s) 5361
Eco47I GGWCC 8 cut(s) 301, 2407, 2432, 4196, 4567, 4706, 5906, 6241
Eco53kI GAGCTC 2 cut(s) 384, 3425
Eco57I CTGAAG 4 cut(s) 459, 1251, 1790, 4274
Eco81I CCTNAGG 2 cut(s) 240, 6204
Eco88I CYCGRG 2 cut(s) 3553, 4409
Eco91I GGTNACC 1 cut(s) 2488
EcoICRI GAGCTC 2 cut(s) 384, 3425
EcoNI CCTNNNNNAGG 1 cut(s) 1305
EcoO109I RGGNCCY 4 cut(s) 563, 2407, 3081, 4567
EcoO65I GGTNACC 1 cut(s) 2488
EcoRI GAATTC 2 cut(s) 771, 2722
EcoRII CCWGG 6 cut(s) 2328, 3321, 4319, 4348, 4527, 6325
EcoRV GATATC 1 cut(s) 5361
EcoT14I CCWWGG 5 cut(s) 1683, 2340, 2444, 4636, 5076
EcoT22I ATGCAT 5 cut(s) 457, 792, 2668, 3319, 6157
EcoT38I GRGCYC 3 cut(s) 386, 566, 3427
ErhI CCWWGG 5 cut(s) 1683, 2340, 2444, 4636, 5076
Esp3I CGTCTC 2 cut(s) 331, 420
FalI AAGNNNNNCTT 6 cut(s) 475, 507, 854, 886, 4704, 4736
FaqI GGGAC 7 cut(s) 2420, 2615, 4302, 4785, 5611, 5635, 6184
FauNDI CATATG 5 cut(s) 1018, 2655, 3517, 4918, 6151
FbaI TGATCA 1 cut(s) 3838
FblI GTMKAC 5 cut(s) 273, 1716, 1939, 4929, 5258
FriOI GRGCYC 3 cut(s) 386, 566, 3427
GlaI GCGC 6 cut(s) 280, 354, 5145, 5755, 5894, 6051
GsaI CCCAGC 2 cut(s) 205, 1172
GsuI CTGGAG 5 cut(s) 284, 400, 1997, 3186, 3693
HaeII RGCGCY 1 cut(s) 356
HapII CCGG 6 cut(s) 27, 315, 805, 1557, 1619, 4410
HgaI GACGC 1 cut(s) 313
HhaI GCGC 6 cut(s) 281, 355, 5146, 5756, 5895, 6052
Hin1I GRCGYC 1 cut(s) 10
Hin6I GCGC 6 cut(s) 279, 353, 5144, 5754, 5893, 6050
HinP1I GCGC 6 cut(s) 279, 353, 5144, 5754, 5893, 6050
HindIII AAGCTT 4 cut(s) 1187, 1835, 2043, 5316
HpaI GTTAAC 4 cut(s) 1066, 1336, 1605, 2464
HpaII CCGG 6 cut(s) 27, 315, 805, 1557, 1619, 4410
HphI GGTGA 9 cut(s) 1205, 2500, 2780, 3337, 3962, 4199, 4283, 5240, 5291
Hpy99I CGWCG 1 cut(s) 287
HpyCH4IV ACGT 6 cut(s) 10, 898, 2274, 2977, 4126, 5107
HpySE526I ACGT 6 cut(s) 10, 898, 2274, 2977, 4126, 5107
Hsp92I GRCGYC 1 cut(s) 10
HspAI GCGC 6 cut(s) 279, 353, 5144, 5754, 5893, 6050
Kpn2I TCCGGA 2 cut(s) 1556, 1618
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 3838
KspAI GTTAAC 4 cut(s) 1066, 1336, 1605, 2464
LguI GCTCTTC 1 cut(s) 543
MaeII ACGT 6 cut(s) 10, 898, 2274, 2977, 4126, 5107
MfeI CAATTG 1 cut(s) 5091
MflI RGATCY 8 cut(s) 757, 1510, 2564, 3182, 3641, 3905, 3940, 4201
MhlI GDGCHC 9 cut(s) 386, 566, 1260, 1994, 2371, 3427, 3610, 4687, 4970
MlsI TGGCCA 2 cut(s) 2313, 6092
MluNI TGGCCA 2 cut(s) 2313, 6092
MlyI GAGTC 8 cut(s) 980, 2246, 3341, 3545, 4727, 4841, 5006, 5633
MmeI TCCRAC 6 cut(s) 279, 3082, 3190, 3601, 4559, 4626
Mox20I TGGCCA 2 cut(s) 2313, 6092
Mph1103I ATGCAT 5 cut(s) 457, 792, 2668, 3319, 6157
MreI CGCCGGCG 1 cut(s) 314
MroI TCCGGA 2 cut(s) 1556, 1618
MroNI GCCGGC 1 cut(s) 314
MroXI GAANNNNTTC 4 cut(s) 487, 490, 828, 2111
MscI TGGCCA 2 cut(s) 2313, 6092
Msp20I TGGCCA 2 cut(s) 2313, 6092
MspA1I CMGCKG 1 cut(s) 2780
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 6 cut(s) 27, 315, 805, 1557, 1619, 4410
MspR9I CCNGG 9 cut(s) 806, 2330, 3323, 4321, 4350, 4410, 4411, 4529, 6327
MunI CAATTG 1 cut(s) 5091
Mva1269I GAATGC 4 cut(s) 455, 1162, 2919, 5729
MvaI CCWGG 6 cut(s) 2330, 3323, 4321, 4350, 4529, 6327
MvnI CGCG 4 cut(s) 279, 287, 346, 5144
NaeI GCCGGC 1 cut(s) 316
NciI CCSGG 3 cut(s) 806, 4410, 4411
NcoI CCATGG 2 cut(s) 2444, 5076
NdeI CATATG 5 cut(s) 1018, 2655, 3517, 4918, 6151
NgoMIV GCCGGC 1 cut(s) 314
NheI GCTAGC 1 cut(s) 1103
NmeAIII GCCGAG 3 cut(s) 308, 3619, 4668
NmuCI GTSAC 7 cut(s) 2397, 2488, 3697, 3830, 5083, 5478, 5611
NruI TCGCGA 1 cut(s) 287
NsiI ATGCAT 5 cut(s) 457, 792, 2668, 3319, 6157
NspI RCATGY 9 cut(s) 1413, 1576, 2011, 2293, 2630, 2666, 2936, 3811, 5583
NspV TTCGAA 2 cut(s) 481, 6098
PaeI GCATGC 1 cut(s) 2011
PagI TCATGA 4 cut(s) 2989, 3912, 4023, 6334
PaqCI CACCTGC 1 cut(s) 397
PciI ACATGT 3 cut(s) 2626, 3807, 5579
PciSI GCTCTTC 1 cut(s) 543
PcsI WCGNNNNNNNCGW 4 cut(s) 279, 3065, 5113, 5538
PctI GAATGC 4 cut(s) 455, 1162, 2919, 5729
PdiI GCCGGC 1 cut(s) 316
PdmI GAANNNNTTC 4 cut(s) 487, 490, 828, 2111
PfeI GAWTC 8 cut(s) 214, 926, 974, 2537, 3461, 3729, 4670, 6224
PflFI GACNNNGTC 1 cut(s) 323
PflMI CCANNNNNTGG 2 cut(s) 1520, 4451
PfoI TCCNGGA 2 cut(s) 804, 4527
PleI GAGTC 8 cut(s) 980, 2246, 3340, 3545, 4727, 4841, 5005, 5632
PpsI GAGTC 8 cut(s) 980, 2246, 3340, 3545, 4727, 4841, 5005, 5632
Ppu21I YACGTR 2 cut(s) 4127, 5108
PpuMI RGGWCCY 2 cut(s) 2407, 4567
PscI ACATGT 3 cut(s) 2626, 3807, 5579
PshAI GACNNNNGTC 2 cut(s) 4996, 5994
PshBI ATTAAT 4 cut(s) 1517, 3027, 4490, 6074
PsiI TTATAA 3 cut(s) 630, 3926, 5801
Psp124BI GAGCTC 2 cut(s) 386, 3427
Psp1406I AACGTT 1 cut(s) 898
Psp5II RGGWCCY 2 cut(s) 2407, 4567
Psp6I CCWGG 6 cut(s) 2328, 3321, 4319, 4348, 4527, 6325
PspEI GGTNACC 1 cut(s) 2488
PspFI CCCAGC 2 cut(s) 201, 1168
PspGI CCWGG 6 cut(s) 2328, 3321, 4319, 4348, 4527, 6325
PspOMI GGGCCC 1 cut(s) 562
PspPPI RGGWCCY 2 cut(s) 2407, 4567
PstI CTGCAG 2 cut(s) 1455, 2875
PstNI CAGNNNCTG 2 cut(s) 2531, 5811
PsuI RGATCY 8 cut(s) 757, 1510, 2564, 3182, 3641, 3905, 3940, 4201
PsyI GACNNNGTC 1 cut(s) 323
PvuII CAGCTG 1 cut(s) 2780
RruI TCGCGA 1 cut(s) 287
SacI GAGCTC 2 cut(s) 386, 3427
SalI GTCGAC 2 cut(s) 272, 5257
SapI GCTCTTC 1 cut(s) 543
SchI GAGTC 8 cut(s) 980, 2246, 3341, 3545, 4727, 4841, 5006, 5633
ScrFI CCNGG 9 cut(s) 806, 2330, 3323, 4321, 4350, 4410, 4411, 4529, 6327
SduI GDGCHC 9 cut(s) 386, 566, 1260, 1994, 2371, 3427, 3610, 4687, 4970
SfcI CTRYAG 8 cut(s) 365, 1451, 1713, 2532, 2871, 5252, 5742, 6392
SfuI TTCGAA 2 cut(s) 481, 6098
SgrAI CRCCGGYG 1 cut(s) 314
SinI GGWCC 8 cut(s) 301, 2407, 2432, 4196, 4567, 4706, 5906, 6241
SmaI CCCGGG 1 cut(s) 4411
SmlI CTYRAG 6 cut(s) 833, 2040, 3476, 4963, 4994, 6163
SmoI CTYRAG 6 cut(s) 833, 2040, 3476, 4963, 4994, 6163
SnaBI TACGTA 2 cut(s) 4127, 5108
SpeI ACTAGT 1 cut(s) 2608
SphI GCATGC 1 cut(s) 2011
SstI GAGCTC 2 cut(s) 386, 3427
StyD4I CCNGG 9 cut(s) 804, 2328, 3321, 4319, 4348, 4408, 4409, 4527, 6325
StyI CCWWGG 5 cut(s) 1683, 2340, 2444, 4636, 5076
TaiI ACGT 6 cut(s) 13, 901, 2277, 2980, 4129, 5110
TaqII GACCGA 2 cut(s) 285, 5237
TatI WGTACW 7 cut(s) 669, 1783, 2260, 2707, 3215, 4135, 5323
TauI GCSGC 4 cut(s) 33, 55, 238, 314
TfiI GAWTC 8 cut(s) 214, 926, 974, 2537, 3461, 3729, 4670, 6224
TseFI GTSAC 7 cut(s) 2397, 2488, 3697, 3830, 5083, 5478, 5611
Tsp45I GTSAC 7 cut(s) 2397, 2488, 3697, 3830, 5083, 5478, 5611
TspGWI ACGGA 5 cut(s) 21, 93, 928, 4522, 6371
TspMI CCCGGG 1 cut(s) 4409
Tth111I GACNNNGTC 1 cut(s) 323
Van91I CCANNNNNTGG 2 cut(s) 1520, 4451
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 8 cut(s) 301, 2407, 2432, 4196, 4567, 4706, 5906, 6241
VspI ATTAAT 4 cut(s) 1517, 3027, 4490, 6074
XagI CCTNNNNNAGG 1 cut(s) 1305
XbaI TCTAGA 2 cut(s) 4292, 5998
XceI RCATGY 9 cut(s) 1413, 1576, 2011, 2293, 2630, 2666, 2936, 3811, 5583
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XmaI CCCGGG 1 cut(s) 4409
XmiI GTMKAC 5 cut(s) 273, 1716, 1939, 4929, 5258
XmnI GAANNNNTTC 4 cut(s) 487, 490, 828, 2111
ZraI GACGTC 1 cut(s) 11
Zsp2I ATGCAT 5 cut(s) 457, 792, 2668, 3319, 6157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.