Rh4DG182600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000582
Physical Location & Seq
Forward (+)
36230301 .. 36251395
21095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG182600.1

Sequence Viewer

Length: 7620 bp
ATGAAGCGACCTGTTGCAGGCAAGGAAAAGCTTGGAATTCGAATATTTAGTTTGGGGGTGCATGCTTCTGCTATGCGTGAAGCCAAGGCTTCAGAACGTGCAGCTTTATGGAAATCATTACAGGAATTAAAGAAAAAGTCTCCCAAAATATCTAGGAGGAAGGCACCAATTTTCTCCTCGCTGAAAAAGCGGCTAGATAAACATTTTCGCGCCATTTCACAGCAATCTGGATTGTGTTCTGTTGTGAATGGAAATGAAGATACACTTGTTTCTTCAAGATCAAAGGATGAAGGTATTAATGATTATTTACATGATGACAAAGGTAATGACCATGGTACTGGTGGTCATATTGACTCACTAAAATCCCTGAAAAGTTCTGACAGAAAGGGTAAGTGTTCTTACTTGTCTGAAGTGGAGGAGAGGTCTTCGCTCATGTCAAACCACCCTTCTCCAAGCCAAAAGCATAATGAGAGTAATGGGTCAGTTAAAAAGAAAAGAAAATACGAAAATCTAAGCTGCCCTATCTCTGTTCCCTCAAAGTTGCGCAAGAGAGAGAAGGTAGAGCAAGATGCTCTTCCTACAGAAAGTGACAGTGAAACTAAGGAGATCAGCGAGATGCTTAAACTTGATCTTTCAATTGCCAATAATCTTTTGAGGATGTTCATTACAACTTGGAAGGAGGCATGTAGGGAGCAGCCTGTGGCTGAGGTACTTTGGGCCATGCTTCGCTCTTGTAATACAAAATATAGGAGGACAAAGAGAATCATGGCAATGTTTTCGACATACCCTTTGGTTGGATTACTCAATGTTGCCGTCTCATCTATCAAATGTGGAATGTGGGATAATATGTTTGATACGTCCCAAACCATTAGTCCACGTGAGTTGGCTAACACTGTAGCTGATAATTATCCTGAACATGTCGAACAAAGTATAAAGGATGCACCACCAGTCAGCACCGAACATGGACAAACCTCTTGCTGCCTGAAGCCAAATCATCAGGATCGTCCACGTCGGTGGAGGAAGATCCCAAGGAGTCGTAAGAAGTTGGAGCCTGAAAACGGACACATCTGCCACCTTTGTTATCAAGAGGGACATGTCAAGAGAAGATGCCCTCTGTGGCAGCAAACTAAAAAATCATCATATGAACCACAGAGTCCTCAACAATTGGCTCAGTATTCTATTTCCGGAGAAGAGGTGGAGGAGAAAGTCAGTGCTGTGGTGGATGACTCCACAGGTGTTTCAGTTAAAGAGATAATCCGGAAAGTTGCTACATATTTTGAGACTGATCAAGGAACGTCTAGCAATGGTAAATCACTTCCGGAGAAGATATTTATTTACTTCAGAAAGCTTTGGAATTGTGAGCTCTGGTTGACTGAAGAATTTTCTGTCAAGGAATTTAGATCCCTTGGTTATGGGGAATTTATGATGTTTTTAGAAAAGTATGCCCGTCTTCTGCCACCGGAGATGTGCAAGTTCTTGAAAGATGACAGCAGTGGAAAGTTTCCTTTGGAGGTTTGCATGCTTCCGCATTATTTAGTTGTTTTGGTATCTCAAGCTTTAAACAATTTATGGGAGGACCAAAAGATTACCAAACTGAACATTTCTTCTCTGCTTGGGAAGCAGTTCCCGGCCCTTAGTTTCCATACCATTGAAAATGGTTCAGTCGAAGACTTTAAAAGTATAGTAGGAAAGCATAAAGACACTGCAATTTCCAAATGTGTACTATTTTCAATGACAATATGCGGAACTAGTTACACTATAGAATCATTGGAAAACTGTAGTAAGAATGCTGTATTGAAAAGCAGTTTGGTGAGTGTTAACAGTGGCCAGAAAATAAGCAATCAATCTGTTGCATCCAAGGAGGCAATTGCAGTCTTACTTAGGGCACCCATGATGTCAGATTTGAATTTATGGTCACATTGGGACCTCTTATTTTCTCCCTCTCTTGGCCCTCTTGTACCGTGGTTATTGAATGAAGTCAACACAGATGAATTACTCTGTTTGGTAACCAAAGATGGCAAAGTTATTCGGTTAGATCATTCAGCTACTGTAGATTCATTCTCGAAAGCTGCTCTTCAAGGATCCTCTTTTCAAACAGCAGTGAAAATGTTATCTCTCTTTTCACTGGTTGGGGGAAAAAAACATGTCCCCGTGTCCCTTTTGAAATTCCATGTACAGCGTGCATTTGAAGTTATTTTCAAGACCTATCTAGATGGTATGGAAGTATATGACAAGAAGAGTTCTCTTAATCAAGGAACAGTTTTGTGTGGACAGAAAATGGTTGTTGAAATTGCTGCTGGTAAATGCAGTAACTTACACAGAGCTGTAACGAAGACGAAAATTGCTGAGTCTGTTATATCACATTTTTTTCTTGATTGCCTGGGGTATCTACCTGCAGAGTTTCGCAGTTTTGCTGCTGATGTGTTACTTTCTGGGATGCGGTCTGTTTTTAAGCATGCAGCTTCAGCCATTTTGGATGAATGCATCCAAATAGAGCAGCGTCTCATACTTCATGAAGTTGGCTTATCTCTTGGTATAGTGGAGTGGATTAGTGATTACCATGTATTTAGTTCCTTGGATTCTACTGATTCGTTCATGTCTGAGGTTTCTGGTCTCAATGCTGATAGAGATCAAATGGGGTCAGACTCAAAATATATGCAAAATGTGTCAAAAAAGTTGACTGCTTCTGAACAGAGTATGGCTGCATCTGTTAGGGCAGTTGAGCATAAAGGAGGATGTGCTGATGTTAGCCGGATGGTTGATGGTGCAGGAGTTTCTGATGCTGGGATTGGCATTGGCATTGGTTACAGACAACATCCTACTAATATTAATGAACATGAAGACGCTCTCCAGGTTATTGAGTCAATCAGGAGAGATGAATTCGGTTTGGATTCAAGCCCATTGACCTTTGAAAGTATCGTCTTAAAGAAACAGCATGCTCGCTTAGGGAGAGCTCTGCACTGTCTTTCACAGGAATTATATTCTCAAGATTCTCATTTTCTTCTTGAGCTGGTTCAAAATGCTGATGATAATACTTATCCAACAAATACAGAACCAACTCTAGCATTCATTCTTCAAGAATCAGGTATTGTTGTTTTGAATAATGAGCAAGGCTTCTCTGCCCAGAACATTAGAGCACTTTGTGATGTTGGAAATTCAACCAAGAAAGGATCCAGTGCCGGATATATAGGGCAAAAGGGCATCGGTTTCAAATCAGTATTTCGTGTCACAGATGCTCCAGAAATTCATTCTAATGGATTTCATATCAAGTTTGATACAAGTGAGGGTCAGATTGGTTTAATTCTGCCCACAGTTGTACCTCCTTGCAATCTTGAGTTGTTTAGACAGCTGACATCTAGTGACATTGATAAACCAGATTGTTACTGCTGGAAAACTTGTATTGTTCTTCCTTTCAGATCGAAAGTATCAGATGGAACTGTCTTGAACCACATAATGAATATGTTTTCAGATCTTCATCCATCTTTACTACTCTTTCTTCACCGCCTCAAGTGTATCCGGTTTAGAAACCTAATCGATGATTCACTTACTGTCATGAGAAAAGAAATTGTAGGAGACGGTATTGTCAAGGTTTCACATGGTAATACGAAGATGACATGGTTTGTAGTATCTCAAAAATTGCACTCAGATAATCTTCGTAGTGATGTGCAAACAACAGAAATCTCAATTGCTTTTACATTGAAGGAATTGGATAAGGGCATTTATCGTCCAGATATAGGCCAACAGCCTGTTTTTGCATTTCTTCCTCTTAGAACATACGGACTGAAATTTATTGTTCAGGGTGATTTTGTTCTTCCTTCATCCAGAGAGGAAGTGGATGGAGATAGTCCCTGGAACCAATGGCTATTGTCAGAATTTCCTGGTTTATTTGTCGATGCAGAGAAATCATTTTGTTCTCTTCCATGTTTCAAAGAGAATCCTGGGACTGCTGTGGCAGCTTACATGTCCTTTGTTCCACTTGTTGGGGAAGTGCATGGTTTCTTTTCTTCTCTTCCTCGATTAATTATTTCTAAATTACGGATGTCAAATTGCTTACTTCAGGAAGGAGAAAAGAAAGAATGGGTTCCTCCTTGCAAGGTCCTAAGAAATTGGAATAAACAGGCTCGTTCACTTCTCCCTGATGGTCTACTGCATGAGCATCTTGGCCTTGGATTCTTGGATAAAGATATAGTTCTGCCTGATCTACTTGCAAGAGTTCTAGGTATTGCAGAGTATGGGCCCACAGTTCTGCTTCAGGTAATGGCTTCTTTATGTGGTAAGCAGGATGGCCTCAAATCAATGGGAATGGGTTGGATGGCCTCTTTGCTTAGTGAACTTTATGCAATGTCATTCAAATCTTCTGTTGAAACTTCCTTTGATTCTGGAATAGAAATGGGATTCCTAGAAAAACTTCAAAAAATTCCATTCATACCTCTTTCAGATGGTACATATGGTGCAGTCGTTGAAGGCCCAATTTGGCTACAGTTTGATGCCGTAGGGACTGGTTTTGGGGATCATCATGGACTTGAATCTTTTCCAAAGTTATATGCTAAACTTCGGATTGTAAGTCCGGAGCTCTATGCATCGTCTGCTGATATGCCATCCATGGATGTGACGCCAATTGACAAAGTCATTAGTATGCTCCATAGAATTGGTGTCCAACGGTTGTCCGCACACGAGATTCTCAAGGCTCACATCTTGCCAGCTATATCTGATGATAGAATTACAGTTAGGGATGAAGATTTGATGACTGATTATATCTGCTTCGCAATGGTTCACTTACAGTCGAGCTGCTCTGATTGTCATGCTGAAAGGGAATACATAATATCAGAACTACAAAATAAAGCTTATATTCTAACTAATAATGGCTTTAAACGACCTGCTGAGGCATCAATTCATTTCAGTAAAGAATTTGGAAATCCAGTGGACATAAGTAGGTTGATTGATAGGGTAAATCTGACATGGGATGAGGTTGATATCTCCTATTTGAAGCATCCAGTTGCTAAATCACTTCCAAATGGACTGATGAAGTGGAGAGATTTTTTCCAGAAAATTGGCATTGTGGATTTTGTGAAGGTAGTTCAAGTTGAGAAGGGTTTCACTGAGTTGTCTGAGGCTCTATTTAATAATCTAACTTGGGACCAGCATACTATTTCCCATGGGTTGAATGCCACAGATTGGGAATCACCTGAGTTAGTCCATTTATTATCCCTATTGTCCAGAGATGGCAACAGAAAAGGTTGTGAGTATCTCTTAGAGGTTCTCGATAAATTATGGGATGATTGCTATAGTGACAGAGCTACAGGGTACTGCTCTTCTAAGTCTGTTGCAGATAGAAAGCCCTTCAAATCTTCATTTATGAACACCATCTGCGATGTGCAATGGGTAGTATCAGGAATGGATGATAAACTTCACTATCCCAAGGATCTATATCACGACAGTGTTGCAGTGCGGTCAATTCTTGGTGGATCTGCCCCTTTTTTTGTTCCTAAGGCTACCAGTGAAAAATTTGTATTTGATATTGGTTTTAAGACAAGAGTCTCTCTTCACGATGCTCTGGAGATGTTGAAACTATGGAGATGTGAGAATCTGTTTAGGGCCAGCCTAGCACAAATGTCCAAATTTTACGCATTAATCTGGAATGAAATGGCTTCTTCAAAGAAGACAACTGCAGAGGAATTCTGTTTGGAACCTTTTGTATTTGTTCCATATGAATCTAGCTTCCGGCATGAGGATGTGGTGTCTGGTACATTTCTGTCCCTTGAAGAAGTATACTGGGATGATTCTACTTTCTTTGTGCACCAAATCAAGGAGATCCATCATCAGTGCAACTCAACAGTTGGTAATCGCCCCATGAACAGGATTCTGAGTAATTTCTACCCGGCCCTTCATGACTTCTTTGTTGATATATGTGGAGTACATGAGATCCCTCCTCTTCGTAGCTACCTTCAGATTTTGCTACAGTTTTCAAATGCTGTTTTGCCTTCACAAGCAGCTAATGCTGTTTTTCAAGTTTTTCAGAAGTGGGCTGATGGGCTCCAATCAGGAATGAGTGCTGAGGATATTGTATACTTGAAAGATTCGCTTACAAGGATGGAATGTACTGTGCTTCCTACTGTACAGGATAAGTGGGTATCTCTGCATCCTTCCTATGGTCTCCTGTGCTGGTGTGATGATAAGACGTTGAAGAAGCAGTTTATGCATATGGATGGACTTGATTTTCTTTACTTTGGTGGACTAAGTAATGATGATGAAGAGATCATAAGTACAAAAATGTCCGTCCTCATGCGAACCTTAGGGATTTCTGCACTTTCAGAGGTTGTGACTCGTGAGGCGATGTTTAATGGTCCGACAGATTCTAGCTTTAAAGCTGCATTGCTGGATTGGGCTCTGCCATATGCGCAGCGCTACTTACATAGTTTACATCCTGATAAATATTCTCAACTCAAGCAGTCTGGATTCGATATACTAAATCGTTTGCGAGTAGTAGAAGTTCAAAAGCTTTCCTACCGCAATGTTATAAAGATTGCTGGAAGTGAATCCAAGAAGCAAATCGAATGTAGTTGTGTTCTGCAGGATCACGATCTGTATACAACCCAGGAATCAGATTCTCACGCATTATTTATGGAGCTTTCTCGTTTGTTTTTTGATGGAAAACCAGAATTACACTTGGCAAATTTCCTTCATATGATCACAACAATGGCTGAATCAGGTTCAACTGAGGAGCAGATAGACTTTTTCATCTTCAATAGCCAAAAGGTGCCAAAGCTTCCTGATGGAGAATCTGTTTGGTCCCTGTCACATGTGCATAATGATAGGTTACCCCAGCCAAGTGACTGGCGCTTTGTTGGTTGGAAAGCCCCACTAAGCTTTGGTTATGCAAATCTTTTCAAGGCTCAGGCACAATTTGCACAGCTTACCAGTGTCTCACAAATTGAAATGGATAATGATTCTGAAACCCATGTCAGACAAACAGTTGAATCAACTCCCATTTCAGTCGACATTAAATGGACAATTGGTGAGGGTACAGCAACAACATCTGCACCTTTAGTGCTGCCAAACTCCAATGATTTGCAGGAACACTTTGATGATGAGACTGATATGGATACTGATTTCAATCCTAATAATCTAGATTTTGTTGTTGACCCTCGCGTTTTGGGTTCATCTGATTGTAGCAAGGTAGACCATCCTAGATATGGCTCTAGTGGAAGAGATTCAATGCGTGGAAGAGATGCAATGCTTACCGGGAGGTTAGGTGAGGTTGTTGCTTTTAAATACTTAATTGCAAAAGCTGGCAAGTCAGTTGTGAGGTGGGTTAATGAAAGAATTGAAACTGGGCTACCCTATGACATAGTTGTAGGTGAGAAGGAAGATAGTCTAGAGTTCATTGAGGTTAAGGCCACCCAACAACAAAAAAAGGATTGGTTCCACATATCAATGAGGGAGTGGCAATTTGCAGCGGAAAAAGGCGAAGCCTTCAGCATTTTGCACGTCCTCTTATTGGGTAATAATGCTGCAAGAGTCTCAGTTTACAAGAATCCTGTAAAATTGTGTCATCTGGGCAAGCTACAGTTGCATCTTTCGATGCCTAGGCAGGAGAAGGAATTATTTCTTGTGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

2539

Amino Acids

285.78

Weight (kDa)

6.27

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 994 - 1092 7.5e-09 Sacsin domain
NOV_C PF13020 2418 - 2504 1.6e-19 Protein NO VEIN, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 6518
AasI GACNNNNNNGTC 1 cut(s) 3581
Acc16I TGCGCA 2 cut(s) 545, 6399
Acc36I ACCTGC 2 cut(s) 2401, 4885
AccB1I GGYRCC 3 cut(s) 163, 1885, 6757
AccB7I CCANNNNNTGG 2 cut(s) 3980, 5175
AccI GTMKAC 6 cut(s) 4144, 5769, 6066, 6587, 6996, 7179
AccII CGCG 2 cut(s) 210, 7149
AccIII TCCGGA 4 cut(s) 1184, 1257, 1318, 4567
AciI CCGC 9 cut(s) 190, 1526, 1743, 2440, 3502, 4668, 5449, 6508, 7457
AcoI YGGCCR 1 cut(s) 1825
AcvI CACGTG 1 cut(s) 878
AcyI GRCGYC 1 cut(s) 4613
AdeI CACNNNGTG 1 cut(s) 3143
AfeI AGCGCT 1 cut(s) 6404
AflIII ACRYGT 5 cut(s) 916, 1093, 2143, 3960, 6799
AhlI ACTAGT 1 cut(s) 1748
AjiI CACGTC 2 cut(s) 1010, 7489
AjnI CCWGG 6 cut(s) 2379, 2850, 3848, 3877, 3937, 6594
AjuI GAANNNNNNNTTGG 2 cut(s) 1790, 1822
AleI CACNNNNGTG 2 cut(s) 1012, 5436
Alw21I GWGCWC 5 cut(s) 1365, 2956, 3139, 4575, 5799
Alw44I GTGCAC 1 cut(s) 5795
AlwNI CAGNNNCTG 1 cut(s) 2048
Aor13HI TCCGGA 4 cut(s) 1184, 1257, 1318, 4567
Aor51HI AGCGCT 1 cut(s) 6404
ApaI GGGCCC 1 cut(s) 4239
ApaLI GTGCAC 1 cut(s) 5795
ArsI GACNNNNNNTTYG 6 cut(s) 772, 804, 856, 888, 6824, 6856
AseI ATTAAT 4 cut(s) 297, 2829, 4019, 5630
Asp700I GAANNNNTTC 7 cut(s) 1622, 4080, 4407, 4530, 5093, 7210, 7605
AspA2I CCTAGG 1 cut(s) 7586
AspLEI GCGC 5 cut(s) 212, 546, 6400, 6405, 6840
AsuC2I CCSGG 3 cut(s) 1628, 5879, 7243
AsuHPI GGTGA 7 cut(s) 1822, 3491, 3812, 5175, 7028, 7265, 7370
AsuII TTCGAA 1 cut(s) 40
AvaII GGWCC 6 cut(s) 1576, 1924, 4096, 5137, 6344, 6789
AvrII CCTAGG 1 cut(s) 7586
AxyI CCTNAGG 2 cut(s) 5487, 6292
BaeGI GKGCMC 3 cut(s) 1888, 4239, 5799
BaeI ACNNNNGTAYC 4 cut(s) 252, 285, 3300, 3333
BalI TGGCCA 1 cut(s) 1827
BamHI GGATCC 2 cut(s) 2081, 3170
BanI GGYRCC 3 cut(s) 163, 1885, 6757
BanII GRGCYC 6 cut(s) 1365, 2956, 4239, 4575, 6036, 6388
BauI CACGAG 2 cut(s) 4673, 6324
BbrPI CACGTG 1 cut(s) 878
BbsI GAAGAC 6 cut(s) 417, 1442, 1674, 2339, 2847, 5666
Bbv12I GWGCWC 5 cut(s) 1365, 2956, 3139, 4575, 5799
BbvCI CCTCAGC 3 cut(s) 705, 4881, 6054
BceAI ACGGC 2 cut(s) 797, 4475
BcgI CGANNNNNNTGC 4 cut(s) 759, 793, 5423, 5457
BciT130I CCWGG 6 cut(s) 2381, 2852, 3850, 3879, 3939, 6596
BciVI GTATCC 2 cut(s) 3524, 7096
BclI TGATCA 2 cut(s) 1285, 6687
BcnI CCSGG 3 cut(s) 1628, 5879, 7243
BcuI ACTAGT 1 cut(s) 1748
BfoI RGCGCY 2 cut(s) 6406, 6841
BfuAI ACCTGC 2 cut(s) 2401, 4885
BfuI GTATCC 2 cut(s) 3524, 7096
BglII AGATCT 1 cut(s) 3469
BlnI CCTAGG 1 cut(s) 7586
Bme1390I CCNGG 9 cut(s) 1628, 2381, 2852, 3850, 3879, 3939, 5879, 6596, 7243
Bme18I GGWCC 6 cut(s) 1576, 1924, 4096, 5137, 6344, 6789
BmgBI CACGTC 2 cut(s) 1010, 7489
BmrFI CCNGG 9 cut(s) 1628, 2381, 2852, 3850, 3879, 3939, 5879, 6596, 7243
BmrI ACTGGG 2 cut(s) 5782, 7341
BmuI ACTGGG 2 cut(s) 5782, 7341
BoxI GACNNNNGTC 1 cut(s) 5534
BpiI GAAGAC 6 cut(s) 417, 1442, 1674, 2339, 2847, 5666
BpmI CTGGAG 3 cut(s) 2834, 3222, 5576
Bpu10I CCTNAGC 5 cut(s) 705, 2944, 4881, 6054, 6894
Bpu14I TTCGAA 1 cut(s) 40
BpuEI CTTGAG 7 cut(s) 1536, 2970, 3026, 3353, 3491, 4667, 6428
BpuMI CCSGG 3 cut(s) 1628, 5879, 7243
Bsa29I ATCGAT 1 cut(s) 3534
BsaAI YACGTR 1 cut(s) 878
BsaBI GATNNNNATC 6 cut(s) 906, 2628, 3474, 5427, 6579, 7113
BsaHI GRCGYC 1 cut(s) 4613
BsaI GGTCTC 2 cut(s) 2618, 6158
BsaWI WCCGGW 6 cut(s) 1184, 1257, 1318, 1459, 3516, 4567
Bse21I CCTNAGG 2 cut(s) 5487, 6292
Bse3DI GCAATG 8 cut(s) 777, 1309, 4347, 4773, 5384, 6371, 6517, 7239
Bse8I GATNNNNATC 6 cut(s) 906, 2628, 3474, 5427, 6579, 7113
BseAI TCCGGA 4 cut(s) 1184, 1257, 1318, 4567
BseBI CCWGG 6 cut(s) 2381, 2852, 3850, 3879, 3939, 6596
BseCI ATCGAT 1 cut(s) 3534
BseJI GATNNNNATC 6 cut(s) 906, 2628, 3474, 5427, 6579, 7113
BseMI GCAATG 8 cut(s) 777, 1309, 4347, 4773, 5384, 6371, 6517, 7239
BseRI GAGGAG 5 cut(s) 166, 431, 1214, 5919, 6734
BseSI GKGCMC 3 cut(s) 1888, 4239, 5799
BseYI CCCAGC 2 cut(s) 2783, 6822
BsgI GTGCAG 6 cut(s) 120, 2787, 2942, 4473, 6288, 7023
Bsh1236I CGCG 2 cut(s) 210, 7149
BshNI GGYRCC 3 cut(s) 163, 1885, 6757
BshVI ATCGAT 1 cut(s) 3534
BsiHKAI GWGCWC 5 cut(s) 1365, 2956, 3139, 4575, 5799
BsmBI CGTCTC 3 cut(s) 820, 2507, 3567
BsmI GAATGC 4 cut(s) 1792, 2486, 3065, 5170
Bso31I GGTCTC 2 cut(s) 2618, 6158
Bsp119I TTCGAA 1 cut(s) 40
Bsp120I GGGCCC 1 cut(s) 4235
Bsp1286I GDGCHC 9 cut(s) 1365, 1888, 2956, 3139, 4239, 4575, 5799, 6036, 6388
Bsp13I TCCGGA 4 cut(s) 1184, 1257, 1318, 4567
Bsp1407I TGTACA 2 cut(s) 2173, 6115
Bsp19I CCATGG 3 cut(s) 331, 4602, 5155
BspACI CCGC 9 cut(s) 190, 1526, 1743, 2440, 3502, 4668, 5449, 6508, 7457
BspDI ATCGAT 1 cut(s) 3534
BspEI TCCGGA 4 cut(s) 1184, 1257, 1318, 4567
BspFNI CGCG 2 cut(s) 210, 7149
BspHI TCATGA 3 cut(s) 2512, 3552, 5887
BspMAI CTGCAG 3 cut(s) 2398, 5671, 6573
BspMI ACCTGC 2 cut(s) 2401, 4885
BspQI GCTCTTC 3 cut(s) 579, 2079, 5317
BspT104I TTCGAA 1 cut(s) 40
BspT107I GGYRCC 3 cut(s) 163, 1885, 6757
BspTNI GGTCTC 2 cut(s) 2618, 6158
BsrDI GCAATG 8 cut(s) 777, 1309, 4347, 4773, 5384, 6371, 6517, 7239
BsrGI TGTACA 2 cut(s) 2173, 6115
BssNAI GTATAC 3 cut(s) 5770, 6067, 6588
BssNI GRCGYC 1 cut(s) 4613
BssSI CACGAG 2 cut(s) 4673, 6324
Bst1107I GTATAC 3 cut(s) 5770, 6067, 6588
Bst2BI CACGAG 2 cut(s) 4673, 6324
Bst2UI CCWGG 6 cut(s) 2381, 2852, 3850, 3879, 3939, 6596
BstACI GRCGYC 1 cut(s) 4613
BstAPI GCANNNNNTGC 4 cut(s) 4586, 5996, 6196, 6905
BstAUI TGTACA 2 cut(s) 2173, 6115
BstBAI YACGTR 1 cut(s) 878
BstBI TTCGAA 1 cut(s) 40
BstDSI CCRYGG 4 cut(s) 331, 1961, 4602, 5155
BstEII GGTNACC 2 cut(s) 2005, 6816
BstENI CCTNNNNNAGG 1 cut(s) 15
BstFNI CGCG 2 cut(s) 210, 7149
BstH2I RGCGCY 2 cut(s) 6406, 6841
BstHHI GCGC 5 cut(s) 212, 546, 6400, 6405, 6840
BstNI CCWGG 6 cut(s) 2381, 2852, 3850, 3879, 3939, 6596
BstPAI GACNNNNGTC 1 cut(s) 5534
BstPI GGTNACC 2 cut(s) 2005, 6816
BstSCI CCNGG 9 cut(s) 1626, 2379, 2850, 3848, 3877, 3937, 5877, 6594, 7241
BstSLI GKGCMC 3 cut(s) 1888, 4239, 5799
BstUI CGCG 2 cut(s) 210, 7149
BstV2I GAAGAC 6 cut(s) 417, 1442, 1674, 2339, 2847, 5666
BstX2I RGATCY 9 cut(s) 1023, 1400, 2081, 3170, 3469, 5422, 5465, 5811, 5922
BstXI CCANNNNNNTGG 5 cut(s) 338, 1014, 4649, 5051, 6834
BstYI RGATCY 9 cut(s) 1023, 1400, 2081, 3170, 3469, 5422, 5465, 5811, 5922
BstZ17I GTATAC 3 cut(s) 5770, 6067, 6588
Bsu15I ATCGAT 1 cut(s) 3534
Bsu36I CCTNAGG 2 cut(s) 5487, 6292
BsuI GTATCC 2 cut(s) 3524, 7096
BsuTUI ATCGAT 1 cut(s) 3534
BtgI CCRYGG 4 cut(s) 331, 1961, 4602, 5155
BtgZI GCGATG 2 cut(s) 5385, 6347
BtrI CACGTC 2 cut(s) 1010, 7489
BtsI GCAGTG 4 cut(s) 1498, 1701, 2106, 5451
BveI ACCTGC 2 cut(s) 2401, 4885
CaiI CAGNNNCTG 1 cut(s) 2048
CciI TCATGA 3 cut(s) 2512, 3552, 5887
CfoI GCGC 5 cut(s) 212, 546, 6400, 6405, 6840
ClaI ATCGAT 1 cut(s) 3534
CseI GACGC 3 cut(s) 2489, 2852, 4621
CspCI CAANNNNNGTGG 2 cut(s) 864, 899
DraI TTTAAA 5 cut(s) 1560, 1675, 4870, 6364, 7270
DraIII CACNNNGTG 1 cut(s) 3143
DrdI GACNNNNNNGTC 1 cut(s) 3581
DseDI GACNNNNNNGTC 1 cut(s) 3581
EaeI YGGCCR 1 cut(s) 1825
Ecl136II GAGCTC 3 cut(s) 1363, 2954, 4573
Eco24I GRGCYC 6 cut(s) 1365, 2956, 4239, 4575, 6036, 6388
Eco31I GGTCTC 2 cut(s) 2618, 6158
Eco32I GATATC 1 cut(s) 4975
Eco47I GGWCC 6 cut(s) 1576, 1924, 4096, 5137, 6344, 6789
Eco47III AGCGCT 1 cut(s) 6404
Eco53kI GAGCTC 3 cut(s) 1363, 2954, 4573
Eco72I CACGTG 1 cut(s) 878
Eco81I CCTNAGG 2 cut(s) 5487, 6292
Eco91I GGTNACC 2 cut(s) 2005, 6816
EcoICRI GAGCTC 3 cut(s) 1363, 2954, 4573
EcoNI CCTNNNNNAGG 1 cut(s) 15
EcoO109I RGGNCCY 2 cut(s) 1924, 4096
EcoO65I GGTNACC 2 cut(s) 2005, 6816
EcoRI GAATTC 3 cut(s) 36, 2879, 5675
EcoRII CCWGG 6 cut(s) 2379, 2850, 3848, 3877, 3937, 6594
EcoRV GATATC 1 cut(s) 4975
EcoT22I ATGCAT 3 cut(s) 2486, 4582, 6201
EcoT38I GRGCYC 6 cut(s) 1365, 2956, 4239, 4575, 6036, 6388
Esp3I CGTCTC 3 cut(s) 820, 2507, 3567
FalI AAGNNNNNCTT 8 cut(s) 249, 281, 558, 590, 2058, 2090, 6066, 6098
FauNDI CATATG 6 cut(s) 1141, 4447, 5707, 6201, 6394, 6684
FbaI TGATCA 2 cut(s) 1285, 6687
FblI GTMKAC 6 cut(s) 4144, 5769, 6066, 6587, 6996, 7179
FriOI GRGCYC 6 cut(s) 1365, 2956, 4239, 4575, 6036, 6388
FspI TGCGCA 2 cut(s) 545, 6399
GlaI GCGC 5 cut(s) 211, 545, 6399, 6404, 6839
GsaI CCCAGC 2 cut(s) 2787, 6826
GsuI CTGGAG 3 cut(s) 2834, 3222, 5576
HaeII RGCGCY 2 cut(s) 6406, 6841
HgaI GACGC 3 cut(s) 2489, 2852, 4621
HhaI GCGC 5 cut(s) 212, 546, 6400, 6405, 6840
Hin1I GRCGYC 1 cut(s) 4613
Hin6I GCGC 5 cut(s) 210, 544, 6398, 6403, 6838
HinP1I GCGC 5 cut(s) 210, 544, 6398, 6403, 6838
HincII GTYRAC 6 cut(s) 1371, 1819, 1981, 2679, 6997, 7141
HindII GTYRAC 6 cut(s) 1371, 1819, 1981, 2679, 6997, 7141
HindIII AAGCTT 7 cut(s) 29, 1346, 1554, 4842, 6497, 6764, 6865
HpaI GTTAAC 1 cut(s) 1819
HphI GGTGA 7 cut(s) 1822, 3491, 3812, 5175, 7028, 7265, 7370
Hpy99I CGWCG 1 cut(s) 1014
HpyCH4IV ACGT 7 cut(s) 97, 857, 877, 1009, 1295, 6179, 7488
HpySE526I ACGT 7 cut(s) 97, 857, 877, 1009, 1295, 6179, 7488
Hsp92I GRCGYC 1 cut(s) 4613
HspAI GCGC 5 cut(s) 210, 544, 6398, 6403, 6838
Kpn2I TCCGGA 4 cut(s) 1184, 1257, 1318, 4567
Ksp22I TGATCA 2 cut(s) 1285, 6687
KspAI GTTAAC 1 cut(s) 1819
LguI GCTCTTC 3 cut(s) 579, 2079, 5317
LmnI GCTCC 8 cut(s) 691, 1048, 3241, 4570, 4644, 6039, 6625, 6721
MaeII ACGT 7 cut(s) 97, 857, 877, 1009, 1295, 6179, 7488
MfeI CAATTG 6 cut(s) 636, 1163, 1866, 3684, 4617, 7011
MflI RGATCY 9 cut(s) 1023, 1400, 2081, 3170, 3469, 5422, 5465, 5811, 5922
MhlI GDGCHC 9 cut(s) 1365, 1888, 2956, 3139, 4239, 4575, 5799, 6036, 6388
MlsI TGGCCA 1 cut(s) 1827
MluNI TGGCCA 1 cut(s) 1827
MmeI TCCRAC 8 cut(s) 775, 1026, 3065, 3130, 4289, 4681, 6371, 6830
Mox20I TGGCCA 1 cut(s) 1827
Mph1103I ATGCAT 3 cut(s) 2486, 4582, 6201
MroI TCCGGA 4 cut(s) 1184, 1257, 1318, 4567
MroXI GAANNNNTTC 7 cut(s) 1622, 4080, 4407, 4530, 5093, 7210, 7605
MscI TGGCCA 1 cut(s) 1827
Msp20I TGGCCA 1 cut(s) 1827
MspA1I CMGCKG 2 cut(s) 3349, 7457
MspR9I CCNGG 9 cut(s) 1628, 2381, 2852, 3850, 3879, 3939, 5879, 6596, 7243
MteI GCGCNGCGC 1 cut(s) 6401
MunI CAATTG 6 cut(s) 636, 1163, 1866, 3684, 4617, 7011
Mva1269I GAATGC 4 cut(s) 1792, 2486, 3065, 5170
MvaI CCWGG 6 cut(s) 2381, 2852, 3850, 3879, 3939, 6596
MvnI CGCG 2 cut(s) 210, 7149
NciI CCSGG 3 cut(s) 1628, 5879, 7243
NcoI CCATGG 3 cut(s) 331, 4602, 5155
NdeI CATATG 6 cut(s) 1141, 4447, 5707, 6201, 6394, 6684
NmuCI GTSAC 9 cut(s) 587, 1914, 3226, 3359, 4609, 5288, 6319, 6795, 6830
NsbI TGCGCA 2 cut(s) 545, 6399
NsiI ATGCAT 3 cut(s) 2486, 4582, 6201
NspV TTCGAA 1 cut(s) 40
OliI CACNNNNGTG 2 cut(s) 1012, 5436
PaeI GCATGC 4 cut(s) 65, 1522, 2459, 2939
PagI TCATGA 3 cut(s) 2512, 3552, 5887
PciI ACATGT 5 cut(s) 916, 1093, 2143, 3960, 6799
PciSI GCTCTTC 3 cut(s) 579, 2079, 5317
PctI GAATGC 4 cut(s) 1792, 2486, 3065, 5170
PdmI GAANNNNTTC 7 cut(s) 1622, 4080, 4407, 4530, 5093, 7210, 7605
PflFI GACNNNGTC 1 cut(s) 4625
PflMI CCANNNNNTGG 2 cut(s) 3980, 5175
PmaCI CACGTG 1 cut(s) 878
PmlI CACGTG 1 cut(s) 878
Ppu21I YACGTR 1 cut(s) 878
PpuMI RGGWCCY 2 cut(s) 1924, 4096
PscI ACATGT 5 cut(s) 916, 1093, 2143, 3960, 6799
PshAI GACNNNNGTC 1 cut(s) 5534
PshBI ATTAAT 4 cut(s) 297, 2829, 4019, 5630
PsiI TTATAA 1 cut(s) 6518
Psp124BI GAGCTC 3 cut(s) 1365, 2956, 4575
Psp5II RGGWCCY 2 cut(s) 1924, 4096
Psp6I CCWGG 6 cut(s) 2379, 2850, 3848, 3877, 3937, 6594
PspCI CACGTG 1 cut(s) 878
PspEI GGTNACC 2 cut(s) 2005, 6816
PspFI CCCAGC 2 cut(s) 2783, 6822
PspGI CCWGG 6 cut(s) 2379, 2850, 3848, 3877, 3937, 6594
PspOMI GGGCCC 1 cut(s) 4235
PspPPI RGGWCCY 2 cut(s) 1924, 4096
PsrI GAACNNNNNNTAC 2 cut(s) 6474, 6506
PstI CTGCAG 3 cut(s) 2398, 5671, 6573
PstNI CAGNNNCTG 1 cut(s) 2048
PsuI RGATCY 9 cut(s) 1023, 1400, 2081, 3170, 3469, 5422, 5465, 5811, 5922
PsyI GACNNNGTC 1 cut(s) 4625
PvuII CAGCTG 1 cut(s) 3349
SacI GAGCTC 3 cut(s) 1365, 2956, 4575
SalI GTCGAC 1 cut(s) 6995
SapI GCTCTTC 3 cut(s) 579, 2079, 5317
ScrFI CCNGG 9 cut(s) 1628, 2381, 2852, 3850, 3879, 3939, 5879, 6596, 7243
SduI GDGCHC 9 cut(s) 1365, 1888, 2956, 3139, 4239, 4575, 5799, 6036, 6388
SfuI TTCGAA 1 cut(s) 40
SinI GGWCC 6 cut(s) 1576, 1924, 4096, 5137, 6344, 6789
SmlI CTYRAG 7 cut(s) 1551, 2985, 3005, 3332, 3506, 4682, 6443
SmoI CTYRAG 7 cut(s) 1551, 2985, 3005, 3332, 3506, 4682, 6443
SpeI ACTAGT 1 cut(s) 1748
SphI GCATGC 4 cut(s) 65, 1522, 2459, 2939
SsiI CCGC 9 cut(s) 190, 1526, 1743, 2440, 3502, 4668, 5449, 6508, 7457
SspI AATATT 3 cut(s) 45, 2827, 6434
SstI GAGCTC 3 cut(s) 1365, 2956, 4575
StyD4I CCNGG 9 cut(s) 1626, 2379, 2850, 3848, 3877, 3937, 5877, 6594, 7241
TaiI ACGT 7 cut(s) 100, 860, 880, 1012, 1298, 6182, 7491
TatI WGTACW 6 cut(s) 1720, 2173, 5914, 6098, 6115, 6263
TauI GCSGC 1 cut(s) 193
TseFI GTSAC 9 cut(s) 587, 1914, 3226, 3359, 4609, 5288, 6319, 6795, 6830
Tsp45I GTSAC 9 cut(s) 587, 1914, 3226, 3359, 4609, 5288, 6319, 6795, 6830
TspGWI ACGGA 4 cut(s) 1074, 3792, 4051, 6265
Tth111I GACNNNGTC 1 cut(s) 4625
Van91I CCANNNNNTGG 2 cut(s) 3980, 5175
VneI GTGCAC 1 cut(s) 5795
VpaK11BI GGWCC 6 cut(s) 1576, 1924, 4096, 5137, 6344, 6789
VspI ATTAAT 4 cut(s) 297, 2829, 4019, 5630
XagI CCTNNNNNAGG 1 cut(s) 15
XbaI TCTAGA 3 cut(s) 2209, 7126, 7375
XcmI CCANNNNNNNNNTGG 3 cut(s) 338, 3829, 7190
XmaJI CCTAGG 1 cut(s) 7586
XmiI GTMKAC 6 cut(s) 4144, 5769, 6066, 6587, 6996, 7179
XmnI GAANNNNTTC 7 cut(s) 1622, 4080, 4407, 4530, 5093, 7210, 7605
Zsp2I ATGCAT 3 cut(s) 2486, 4582, 6201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.