Rroxscaffold_7G00193450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000582
Physical Location & Seq
Reverse (-)
34962607 .. 34979371
16765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00193450.1

Sequence Viewer

Length: 6639 bp
ATGTTTTCTTTGGATCCATTAATTGGATTGTTAAATGTTGCTGTCTCATCTATCAAATCCGGATTGTGGGATAGTATGTATGATACATTCCAAACTGTTGGTCAGTTAACTGACAGTCTTCCGGATAACTGTCCTGAATATGTGAACATAGATGTTGAACCAAGTATTAAAGATGTGAAAAGTACCAAGGATGCACCAGTCATTCCTGAACACGCTGTAGACCACAGGCAGAGTGTTTCTGTTGAGGACATAATCAGGAAACTTACAATGTATTTTGAGATTGATCGAGGTGTACATGGCAATGGTAAATCACTTCAGGAGAAAATATGTACCTTCTTGACAAAGCTTTACAGTTGTGAACTGTGGTTAGTGGAAGAATTTTCTGTCAAGGAATTTAGATCGCTTGGTCATGGAGAGTTTTTAAGGTTCTTAGAAAATTATGCTGGTCTACTTCCACAAGACCTGTGCAAGTTCCTGACTGATGATGTAATTGAGAAGTGCCCTTTGGAAGTTTGCATGCTCCAGCACCATTTGGTTGTTTTACTATCTCAAGCATTAAATAGTTTATGGGAGGATGAAAATATCACAAAACAGGACATTCTGTTGCTGCTTAGGAAGCAATTCCCATTGGTTAGTTTCAAAACTATTGAAAATGGTTCAGTAGAAGATTTTTTAAGCATAGTAGGAAAGCATAAAAATGATGTGATTTCCAAATGTGTTCTGTTCTCAATGGCATTACATGGCACTAGCTATGGGATAGACTCATCAGTACATTATGAGAACGTCCTATTGAAAAGCATGTCATTGAGTTCTGACAGTGGCCAGAAAGCAAAAATCCATGTTGCATCCAAGGATGCAATTAAAGTCTTAGCTAGGGCACCAATGATGTCTGACTTAAACTTATGGTCACACTGGGACCTTTTGTTTGCTCCTTCACTTGGTCCTCTGGTACCATGGTTATTAAATGAAGTTAACACAGATGAATTGTTGTGTTTGGTGACCAAAGATGGAAAAGTCTTTCGGTTAGACCAATCAGCTACTGTAGATTCCTTCGTGGAAGCTGCCCTTCAAGGATCCTCTTTCCAAACAGCAGTGAAATTGTTATCTCTATTTTCACTAGTTGGGGGAGAAAAACATGTCCCTGTGCCTCTTTTGAAAATCCACATGCAACATGCATTTGAAGTCATTTTGAAAAATTTTGTAGATGACATGGTAGTACATGACAATAAGAATTCTCTTAATTATGGAAAATCGTTGTCTGGACAACAAATGATTGGTGAAATTGCAGCTGGTAAACTATCACACAGAGACTTACACAAGACAGATATTGGAAAACCCATTATATCAAGATTTGTCCTTGAATGTCTTGGGTATTTACCTGCAGAATTTCGTGGTTTTGCTGCTGATGTATTGCTTTCGGGAATGCAGTCTGTTGTCAAACATGCTGCTTCGGCCATATTGAGTGAGTGCAGCCAATCAGAGCAGCGTATCATGCTTCATGAAGTCGGTTTGTCTCTTGGTATAGTTGAGTGGATTAATGATTACTACACATTTCTTTCCAATAATACGGCTCAGTCGTTCATATCAGGGGCCTCATGTTCCAATGCTGTTGGATGTGAAATGGGGTCAGGTTCAAAGCCCTTGCAAGATGTTTCAGATAAGTTTTTGACTTCTGGAGGGAGCATGGCTGGATCTGTTAGGGAAGATGTGCAGGAAGTTGGATGTACTGATGTCTCTCTGAAAATTGGTGGTGCGGACATCTCTGATGAGAGAGCTGGCAGTGGTTACACACAACATTCTGCCAAAATTAGTGAATATGAAGATGCATCCCAGGTGATTGAGTCAATCAGAAGAGATGAATTTGGTCTTCATTCAGGCCAAACCACCTCTGAAAGTATCATGTTGAAGAAGCATCATGCTCGCTTAGGGAGAGCTCTACATTGTCTTTCACAGGAATTGTATTCTCAGGATTCACATTTTCTTCTTGAGCTGGTTCAAAATGCAGATGATAATACATACCCAACATATGTGGAACCAACTTTAACATTCATTCTTCAAGACTCGGGTATTATTGTTTTAAATAATGAGCAAGGCTTCGCTGCCGAGAACATTAGAGCACTTTGTGATGTTGGAAGTTCAACCAAGAAAGGATCTAATGCTGGCTATATTGGGCGGAAGGGCATTGGTTTCAAATCTGTGTTTCGGGTCACAGATGCTCCAGAAATTCATTCCAATGGATTCCATATCAAGTTTGATATAAGTCAGGGTCAGATTGGTTTTCTTCTGCCAACAGTTGTACCTCCTTGCAACATTGACATGTTAAGTAGGCTGACTTCTAGTGACAGTGATCAACAGGACAGTAACTTCTGGAACACTTGTATTGTTCTTCCTTTCAGATCGAAGTTTTCAGATGGATCTGGCATGAAAAGCATTATAAGTATGTTTTCAGATCTTCATCCATCTTTATTACTCTTCCTTCACCGCCTCCAATGTATAAAGTTTAGAAACTTGCTTGATAATTCACTGACTGTCATGAGAAAAGAAACTGTGGGAGATGGTATAGTGAAGGTTTCACATGGGAAAGAGAAAATGACATGGTTCTTAGTATCTCAGAAATTGCAAGCGGATTTTATACGTAGTGATGTACAAACAACAGAAATCTCCATAGCATTTACATTGAAGGAATTAGATAATGGTGATTATGGACCAGATCTAGGGCAACAGCCTGCTTTTGCATTTCTTCCTCTAAGAACCTATGGCCTGAAGTTTATTGTCCAGGGTGATTTTGTTCTTCCTTCATCTAGAGAGGAAGTGGATGGAGATAGTCCCTGGAACCAGTGGCTATTATCAGAATTTCCTGGTTTATTTGTCAATGCTGAGAGGTCGTTTTGTTCTCTTCCCTGCTTTAGAGAGAATCCTGGGAGAGCAGTGGCAGCTTATATGTCATTTGTTCCACTTGTTGGGGAAGTGCATGGGTTTTTTTCTTCTCTTCCTCGATTAATCATTTCTAAATTACGGATGTCAAATTGCTTACTCCTGGAAGGAGGAAATAACGAGTGGGTTCCTCCTTGCAAGGTCCTAAGAGGTTGGAATGAGCAGGCCCGATTGCTTCTGCCTGATAGTTTACTTCGGGAACATCTTGGCGTTGGGTTGTTGGATAAAAATATAGTTCTGCCTGATCCACTTGCAAGAGCACTCGGCATTGCAGACTATGGACCCAAAGTTCTGCTTCAAGTTATGGACTCCTTATGTCGTTTGCAAAATGGCCTCACATCAATGGGACTGGGTTGGTTGACCTCTTGGCTAAGTGAACTGTATGCAATGTCATTCAATGCTTCTGTGGAAACATCATTTGACTCTGGACTTGGAATGGATTTGATAGAAGAACTCCGAAAAATTCCATTCATTCCTCTTTCAGATGGTACATATGGTGCAGTAGACAAAGACCCAATTTGGTTACATTTTGATGCCTTGAGCACTGGGTTTGAGGGTCAGCATGGACTTGAGTCTTTTCCTAACTTGTATGCTAATCTTCGTATCGTAAGCCCTGCCTTCCTTTCGACATCATGTGTAGATATGTCATCCATGGATGTGACTACAATTGACAAACAAATGCGTATGCTCCATAGAATTGGTGTCCAGCAGTTGTCCGCGCATGAAATTGTCAAGGTACACATCTTGCCAGCTATATCTGATAACAGAAATGCAGCTAGGGATAAGAATTTGATGACCGAATATCTCTGCTTTGTAATGGTTCACCTACAGTCGACCTGCTCCGATTGTCATGGTGAAAAGGAATACATAATGTCAGAATTACGAAATAAAGCTTATATACTAACAAATCATGGCTATAAACGACCTGCTGAGATATCTATTCATTTCAGTAAGGAATATGGAAATCCTATCGACATAAATAAGTTGATAAATATGGTGGATATGATGTGGCATGAGGTTGAAATCTCCTATTTGAAGCATCCAGTTACAAAGTCACTCCAATGTGGACTAACGAAGTGGAGGGAATTTTTCCAGCAAATTGGCATCGTGGATTTTGTGAAAGTAGTTCACATTGAAAAAGGGTTTAATGATACATGTAAGGACTTGGTTTCTCTTGGATCAAATGTCACAGATTGGGAGTCCCCTGAATTGGTCAGTTTATTGTCCCTGTTGACCAGAACTGGCAACAAAAAAGGCTGTGAATATCTTTTACTGGTGCTTGATAGTTTATGGGATGAATGCTATCTTGAAAAAGCAACAGGCTACTGCGCTTCTAAAGATGTATTGGAGAAAAAGCCTTTTAAATCTTCATTTATAAGCAGCATCTGTGATGCCCAATGGGTAGTGTCAACAATGGATGATGCACTTCACTATCCTAGAGATTTATACCACGATTGTGATGCAGTGCGCTCGGTTCTTGGTCCATCTGCTCCATTTTCTGTTCCAAAGATAGGGAGTGGAAATTTTGCAAGTGCTATGGGGTTAAAGACATTAGTTTCTCTTGACGATGGTCTAGAGGTATTGAAACTATGGAGGTGTAATAATCCATTCAGGGCCAGCATAGCGCAAATGTTCAAATTTTACACATTAATCTGGAACGAAATGGCCACTTCGAAGCCGAGAGTTTTAGAGGAGTTCCACTCCAAACCTTCTATTTTTGTTCCATACGCATCTAGCTCAAGGCATGAGGATGTGGTGTCTGGTACATTTTTGTCCCCTGAGGAAGTATATTGGTATGATTCGACTTCCTTCGTGGACCAAATCAAGCATATACATCCTCAGTGCAGCCCAACAGGTGTTACTCATGGCCCCTTGAATAAGACATTGTGCAACTTTTATCCTGGCCTTCATGACTTCTTTGTTGATGGATGTGGAGTACCTGAGACCCCTCCTCTCCGTAGCTACCTACAGATTTTGCTGCATTTATCAAAGGTTGCTCTGCCTTCACAAGCAGCTAATGCCGTTTTTCAAGTCTTTCTTAAGTGGACTGATGGACTGAAATCAGGAATGAGCCCTGAGGATATTGTTTACATTAGAGATTCTCTTCAGAAGATGGAATGCATGGTGCTTCCTACTGTACATGATAAGTGGGTTTCTTTACATCCATCCTTCGGTCTTGTATGCTGGTGTGATGAGAAGAAATTAAGCAAGCAGTTTAAGCATTTGGATGGTATTGATTTTTTATACTTTGGTCAATTGACCAAGGACAATGAAGAGATACTACATACGAAAGTGTCGAACCTCATGCAAACTTTAGGGATTCCTGCTCTTTCACAGGTTGTGACTCGTGAGGCAATATATTATGGCCTGCAAGACAGTAGCAATGAAGCTGCATTGGTAAATTCAGCTCTTCCATATGCACAGCGCTACTTGCATAGTCTGCACCCAGATAAATATTCTCAACTCAAGCAGTCTGGATTTGATATCCTAAATTGTTTGCAAGTAGTAGTTGTTGAAGAGCTGTACTATCGAAATGTTGTAAGAGTTGCTGGAAGTGAATCTAAGAAGCGGGTTGCATGTAGTTGTCTTCTGAAGGGTAGCATGTTGTATACAACCCGAGAATCAGATTCTCACACATTATTTATGGAACTTTCTCGTTTGTTTTTTAATGGAAATCCTGAACTGCACTTGGCGAATTTCCTTCATATCATCACAACCATGGAAAAATCAGGTTCGAACGAGGAGCAGATAGAGTTATTTATCCTCAATAGCCAAAAGGTGCCGAAGCTTCCTGATGGGGAATGTGTTTGGTCCCTTTCTTCTTTGCACTCATTGACTGAGGATGATGAATCACTCCAGACAAGTACTACTTCAATGGAAGCAAAAGAACAAAACAGTTGGAAACCCAAAAGGAAAGCAGAAAACTGGCCGCCTGCTGATTGGAAAACTGCACCAGGGTTTGCGTATGCTCGTGCACATGGTTTTAAGACACAGCCAACAGTTTTACAGCCCTATGGCGCCTTACAAAATAAGATGGATGATGATTCTGAAGGAATTGTTGGACAAATAGATAATTTGGCTCACAGTTCTGTGGATGCTAATTGGACGTTGGAAGATTATTCAGCAACTGCGTCTTTAGCTTTGCCAGACAATAATGATTTGCTAGAACATCGTGGGGAGCATTTCAATGACTCTGACTTTGCATCACATAATGAATTTGATCCCATCAATCTCGGTCTTGTTTCCGACCCTCCAGAGTTGGGCTCATCCAATGTTGGCAAGAGAGAGCAGCTTAGATATGGCACACCCAATGCTACACAAGCAATTATGACTGGGAGACTTGGAGAGCTTGTTGCTTTTAAATATTTTGTTGAGAAAGGCGGCAAGTCAGCAGTCAAGTGGGTTAATGAGCACAATGAAACAGGGCTACCCTATGACATAGTGGTGGGGGAGAACAATAAGGAGTACATTGAAGTGAAAGCAACGAAATCTGCAAGAAAAGATTGGTTTGAAATATCAATGAATGAGTTGCAATTTGCAGTTGAGAAAGGTGAAGCTTTTAGCATAGCTCATGTCATCTTATTAGATAATAATGTTGCAAAGGTCAGAGTGTACAACAATCTGGCAAAGTTATGCCAGCTTCGCCAACTGAAGTTAGCTGTATTGGTGCCTGTGCAGCAGAAGGAATTCGCCATTGTATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

2212

Amino Acids

247.1

Weight (kDa)

5.55

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 654 - 839 5.4e-09 Sacsin domain
NOV_C PF13020 2093 - 2176 3e-19 Protein NO VEIN, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2435, 4310
Acc36I ACCTGC 3 cut(s) 1387, 3779, 3868
Acc65I GGTACC 1 cut(s) 949
AccB1I GGYRCC 5 cut(s) 877, 949, 5713, 5951, 6601
AccB7I CCANNNNNTGG 2 cut(s) 23, 2960
AccI GTMKAC 5 cut(s) 219, 448, 3438, 3767, 5543
AccII CGCG 1 cut(s) 3653
AccIII TCCGGA 2 cut(s) 59, 121
AciI CCGC 8 cut(s) 1754, 2173, 2482, 2624, 3651, 5503, 5864, 6315
AcoI YGGCCR 4 cut(s) 820, 1452, 4599, 5861
AcuI CTGAAG 6 cut(s) 299, 2783, 5024, 5546, 6003, 6605
AcyI GRCGYC 1 cut(s) 5952
AdeI CACNNNGTG 1 cut(s) 2123
AfeI AGCGCT 1 cut(s) 5360
AfiI CCNNNNNNNGG 9 cut(s) 23, 66, 938, 2714, 2960, 4144, 4445, 5105, 6194
AflII CTTAAG 1 cut(s) 4973
AflIII ACRYGT 3 cut(s) 1135, 2316, 4088
AhlI ACTAGT 1 cut(s) 1117
AjnI CCWGG 8 cut(s) 1830, 2775, 2828, 2857, 2917, 3036, 4834, 5887
AjuI GAANNNNNNNTTGG 4 cut(s) 488, 520, 5976, 6008
AleI CACNNNNGTG 1 cut(s) 4389
AloI GAACNNNNNNTCC 2 cut(s) 3207, 3239
Alw21I GWGCWC 6 cut(s) 1936, 2119, 3196, 3479, 5911, 6348
Alw26I GTCTC 6 cut(s) 49, 1302, 1518, 1738, 4871, 6265
Alw44I GTGCAC 1 cut(s) 5907
AlwNI CAGNNNCTG 3 cut(s) 1040, 4320, 6062
Ama87I CYCGRG 2 cut(s) 2062, 5550
Aor13HI TCCGGA 2 cut(s) 59, 121
Aor51HI AGCGCT 1 cut(s) 5360
ApaLI GTGCAC 1 cut(s) 5907
ArsI GACNNNNNNTTYG 6 cut(s) 85, 117, 908, 940, 6116, 6148
AseI ATTAAT 4 cut(s) 20, 1536, 2999, 4583
Asp700I GAANNNNTTC 2 cut(s) 620, 6620
Asp718I GGTACC 1 cut(s) 949
AspLEI GCGC 6 cut(s) 3655, 4265, 4404, 4561, 5361, 5954
AsuHPI GGTGA 9 cut(s) 1009, 1289, 1846, 2471, 2708, 2792, 3749, 3800, 6497
AsuII TTCGAA 2 cut(s) 4607, 5669
AvaI CYCGRG 2 cut(s) 2062, 5550
AvaII GGWCC 8 cut(s) 916, 941, 2705, 3076, 3215, 4415, 4750, 5745
AxyI CCTNAGG 2 cut(s) 4713, 5010
BaeGI GKGCMC 3 cut(s) 503, 880, 5911
BaeI ACNNNNGTAYC 4 cut(s) 941, 974, 2280, 2313
BalI TGGCCA 2 cut(s) 822, 4601
BamHI GGATCC 2 cut(s) 13, 1073
BanI GGYRCC 5 cut(s) 877, 949, 5713, 5951, 6601
BanII GRGCYC 3 cut(s) 1936, 5009, 6200
BarI GAAGNNNNNNTAC 4 cut(s) 2317, 2349, 5199, 5231
BauI CACGAG 2 cut(s) 5280, 5904
BbsI GAAGAC 3 cut(s) 110, 1859, 5513
Bbv12I GWGCWC 6 cut(s) 1936, 2119, 3196, 3479, 5911, 6348
BceAI ACGGC 2 cut(s) 1584, 4940
BcgI CGANNNNNNTGC 8 cut(s) 1901, 1935, 4376, 4386, 4410, 4420, 5221, 5255
BciT130I CCWGG 8 cut(s) 1832, 2777, 2830, 2859, 2919, 3038, 4836, 5889
BclI TGATCA 1 cut(s) 2347
BcoDI GTCTC 6 cut(s) 49, 1302, 1518, 1738, 4871, 6265
BcuI ACTAGT 1 cut(s) 1117
BfmI CTRYAG 5 cut(s) 216, 1041, 1380, 3761, 4901
BfoI RGCGCY 2 cut(s) 5362, 5955
BfrI CTTAAG 1 cut(s) 4973
BfuAI ACCTGC 3 cut(s) 1387, 3779, 3868
BglII AGATCT 2 cut(s) 2449, 2710
BmcAI AGTACT 1 cut(s) 5800
Bme1390I CCNGG 8 cut(s) 1832, 2777, 2830, 2859, 2919, 3038, 4836, 5889
Bme18I GGWCC 8 cut(s) 916, 941, 2705, 3076, 3215, 4415, 4750, 5745
BmeT110I CYCGRG 2 cut(s) 2062, 5550
BmrFI CCNGG 8 cut(s) 1832, 2777, 2830, 2859, 2919, 3038, 4836, 5889
BmrI ACTGGG 4 cut(s) 922, 3293, 3489, 6276
BmuI ACTGGG 4 cut(s) 922, 3293, 3489, 6276
BoxI GACNNNNGTC 2 cut(s) 3505, 4503
BpiI GAAGAC 3 cut(s) 110, 1859, 5513
BplI GAGNNNNNCTC 4 cut(s) 4619, 4651, 6182, 6214
BpmI CTGGAG 5 cut(s) 506, 1695, 2202, 5774, 6171
Bpu10I CCTNAGC 2 cut(s) 611, 1924
Bpu14I TTCGAA 2 cut(s) 4607, 5669
BpuEI CTTGAG 6 cut(s) 534, 2006, 3493, 3524, 4657, 5384
BsaAI YACGTR 1 cut(s) 2636
BsaBI GATNNNNATC 1 cut(s) 2454
BsaHI GRCGYC 1 cut(s) 5952
BsaI GGTCTC 1 cut(s) 4871
BsaWI WCCGGW 2 cut(s) 59, 121
BsaXI ACNNNNNCTCC 4 cut(s) 2200, 2230, 6270, 6300
Bsc4I CCNNNNNNNGG 9 cut(s) 23, 66, 938, 2714, 2960, 4144, 4445, 5105, 6194
Bse21I CCTNAGG 2 cut(s) 4713, 5010
Bse3DI GCAATG 4 cut(s) 307, 3201, 3327, 5323
Bse8I GATNNNNATC 1 cut(s) 2454
BseAI TCCGGA 2 cut(s) 59, 121
BseBI CCWGG 8 cut(s) 1832, 2777, 2830, 2859, 2919, 3038, 4836, 5889
BseJI GATNNNNATC 1 cut(s) 2454
BseLI CCNNNNNNNGG 9 cut(s) 23, 66, 938, 2714, 2960, 4144, 4445, 5105, 6194
BseMI GCAATG 4 cut(s) 307, 3201, 3327, 5323
BseRI GAGGAG 3 cut(s) 4640, 4875, 5690
BseSI GKGCMC 3 cut(s) 503, 880, 5911
BsgI GTGCAG 8 cut(s) 1489, 1730, 3453, 4798, 5360, 5603, 5868, 6629
Bsh1236I CGCG 1 cut(s) 3653
BshNI GGYRCC 5 cut(s) 877, 949, 5713, 5951, 6601
BsiHKAI GWGCWC 6 cut(s) 1936, 2119, 3196, 3479, 5911, 6348
BsiHKCI CYCGRG 2 cut(s) 2062, 5550
BsiSI CCGG 2 cut(s) 60, 122
BslFI GGGAC 8 cut(s) 929, 1124, 2811, 3294, 4120, 4144, 4693, 5731
BslI CCNNNNNNNGG 9 cut(s) 23, 66, 938, 2714, 2960, 4144, 4445, 5105, 6194
BsmAI GTCTC 6 cut(s) 49, 1302, 1518, 1738, 4871, 6265
BsmFI GGGAC 8 cut(s) 929, 1124, 2811, 3294, 4120, 4144, 4693, 5731
BsmI GAATGC 3 cut(s) 1428, 4238, 5057
Bso31I GGTCTC 1 cut(s) 4871
BsoBI CYCGRG 2 cut(s) 2062, 5550
Bsp119I TTCGAA 2 cut(s) 4607, 5669
Bsp13I TCCGGA 2 cut(s) 59, 121
Bsp1407I TGTACA 4 cut(s) 292, 2644, 5071, 6546
Bsp19I CCATGG 3 cut(s) 953, 3585, 5652
BspACI CCGC 8 cut(s) 1754, 2173, 2482, 2624, 3651, 5503, 5864, 6315
BspEI TCCGGA 2 cut(s) 59, 121
BspFNI CGCG 1 cut(s) 3653
BspHI TCATGA 3 cut(s) 1498, 2532, 4843
BspMAI CTGCAG 1 cut(s) 1384
BspMI ACCTGC 3 cut(s) 1387, 3779, 3868
BspQI GCTCTTC 2 cut(s) 5349, 5445
BspT104I TTCGAA 2 cut(s) 4607, 5669
BspT107I GGYRCC 5 cut(s) 877, 949, 5713, 5951, 6601
BspTI CTTAAG 1 cut(s) 4973
BspTNI GGTCTC 1 cut(s) 4871
BsrDI GCAATG 4 cut(s) 307, 3201, 3327, 5323
BsrGI TGTACA 4 cut(s) 292, 2644, 5071, 6546
BssNAI GTATAC 1 cut(s) 5544
BssNI GRCGYC 1 cut(s) 5952
BssSI CACGAG 2 cut(s) 5280, 5904
BssT1I CCWWGG 6 cut(s) 186, 849, 953, 3585, 5196, 5652
Bst1107I GTATAC 1 cut(s) 5544
Bst2BI CACGAG 2 cut(s) 5280, 5904
Bst2UI CCWGG 8 cut(s) 1832, 2777, 2830, 2859, 2919, 3038, 4836, 5889
Bst6I CTCTTC 8 cut(s) 1846, 2477, 2901, 2994, 5043, 5202, 5349, 5445
BstACI GRCGYC 1 cut(s) 5952
BstAFI CTTAAG 1 cut(s) 4973
BstAPI GCANNNNNTGC 2 cut(s) 4952, 5374
BstAUI TGTACA 4 cut(s) 292, 2644, 5071, 6546
BstBAI YACGTR 1 cut(s) 2636
BstBI TTCGAA 2 cut(s) 4607, 5669
BstDSI CCRYGG 3 cut(s) 953, 3585, 5652
BstEII GGTNACC 1 cut(s) 997
BstFNI CGCG 1 cut(s) 3653
BstH2I RGCGCY 2 cut(s) 5362, 5955
BstHHI GCGC 6 cut(s) 3655, 4265, 4404, 4561, 5361, 5954
BstMAI GTCTC 6 cut(s) 49, 1302, 1518, 1738, 4871, 6265
BstNI CCWGG 8 cut(s) 1832, 2777, 2830, 2859, 2919, 3038, 4836, 5889
BstPAI GACNNNNGTC 2 cut(s) 3505, 4503
BstPI GGTNACC 1 cut(s) 997
BstSCI CCNGG 8 cut(s) 1830, 2775, 2828, 2857, 2917, 3036, 4834, 5887
BstSFI CTRYAG 5 cut(s) 216, 1041, 1380, 3761, 4901
BstSLI GKGCMC 3 cut(s) 503, 880, 5911
BstSNI TACGTA 1 cut(s) 2636
BstUI CGCG 1 cut(s) 3653
BstV2I GAAGAC 3 cut(s) 110, 1859, 5513
BstX2I RGATCY 7 cut(s) 13, 1073, 1691, 2150, 2414, 2449, 2710
BstXI CCANNNNNNTGG 3 cut(s) 98, 3632, 4034
BstYI RGATCY 7 cut(s) 13, 1073, 1691, 2150, 2414, 2449, 2710
BstZ17I GTATAC 1 cut(s) 5544
Bsu36I CCTNAGG 2 cut(s) 4713, 5010
BtgI CCRYGG 3 cut(s) 953, 3585, 5652
BtsI GCAGTG 4 cut(s) 1098, 1786, 2934, 4404
BveI ACCTGC 3 cut(s) 1387, 3779, 3868
CaiI CAGNNNCTG 3 cut(s) 1040, 4320, 6062
CciI TCATGA 3 cut(s) 1498, 2532, 4843
CfoI GCGC 6 cut(s) 3655, 4265, 4404, 4561, 5361, 5954
CseI GACGC 1 cut(s) 6054
CspCI CAANNNNNGTGG 2 cut(s) 2010, 2045
DinI GGCGCC 1 cut(s) 5953
DraI TTTAAA 3 cut(s) 2079, 4297, 6295
DraIII CACNNNGTG 1 cut(s) 2123
EaeI YGGCCR 4 cut(s) 820, 1452, 4599, 5861
Eam1104I CTCTTC 8 cut(s) 1846, 2477, 2901, 2994, 5043, 5202, 5349, 5445
EarI CTCTTC 8 cut(s) 1846, 2477, 2901, 2994, 5043, 5202, 5349, 5445
EciI GGCGGA 1 cut(s) 2188
Ecl136II GAGCTC 1 cut(s) 1934
Eco105I TACGTA 1 cut(s) 2636
Eco130I CCWWGG 6 cut(s) 186, 849, 953, 3585, 5196, 5652
Eco24I GRGCYC 3 cut(s) 1936, 5009, 6200
Eco31I GGTCTC 1 cut(s) 4871
Eco32I GATATC 2 cut(s) 3870, 5419
Eco47I GGWCC 8 cut(s) 916, 941, 2705, 3076, 3215, 4415, 4750, 5745
Eco47III AGCGCT 1 cut(s) 5360
Eco53kI GAGCTC 1 cut(s) 1934
Eco57I CTGAAG 6 cut(s) 299, 2783, 5024, 5546, 6003, 6605
Eco81I CCTNAGG 2 cut(s) 4713, 5010
Eco88I CYCGRG 2 cut(s) 2062, 5550
Eco91I GGTNACC 1 cut(s) 997
EcoICRI GAGCTC 1 cut(s) 1934
EcoO109I RGGNCCY 3 cut(s) 916, 1590, 3076
EcoO65I GGTNACC 1 cut(s) 997
EcoRI GAATTC 2 cut(s) 1231, 6620
EcoRII CCWGG 8 cut(s) 1830, 2775, 2828, 2857, 2917, 3036, 4834, 5887
EcoRV GATATC 2 cut(s) 3870, 5419
EcoT14I CCWWGG 6 cut(s) 186, 849, 953, 3585, 5196, 5652
EcoT22I ATGCAT 3 cut(s) 1177, 1828, 5057
EcoT38I GRGCYC 3 cut(s) 1936, 5009, 6200
EgeI GGCGCC 1 cut(s) 5953
EheI GGCGCC 1 cut(s) 5953
ErhI CCWWGG 6 cut(s) 186, 849, 953, 3585, 5196, 5652
FalI AAGNNNNNCTT 8 cut(s) 1050, 1082, 3213, 3245, 4956, 4988, 5788, 5820
FaqI GGGAC 8 cut(s) 929, 1124, 2811, 3294, 4120, 4144, 4693, 5731
FauI CCCGC 1 cut(s) 5496
FauNDI CATATG 3 cut(s) 2026, 3427, 5350
FbaI TGATCA 1 cut(s) 2347
FblI GTMKAC 5 cut(s) 219, 448, 3438, 3767, 5543
FriOI GRGCYC 3 cut(s) 1936, 5009, 6200
GlaI GCGC 6 cut(s) 3654, 4264, 4403, 4560, 5360, 5953
GsuI CTGGAG 5 cut(s) 506, 1695, 2202, 5774, 6171
HaeII RGCGCY 2 cut(s) 5362, 5955
HapII CCGG 2 cut(s) 60, 122
HgaI GACGC 1 cut(s) 6054
HhaI GCGC 6 cut(s) 3655, 4265, 4404, 4561, 5361, 5954
Hin1I GRCGYC 1 cut(s) 5952
Hin6I GCGC 6 cut(s) 3653, 4263, 4402, 4559, 5359, 5952
HinP1I GCGC 6 cut(s) 3653, 4263, 4402, 4559, 5359, 5952
HincII GTYRAC 6 cut(s) 108, 973, 3294, 3768, 4167, 4344
HindII GTYRAC 6 cut(s) 108, 973, 3294, 3768, 4167, 4344
HindIII AAGCTT 4 cut(s) 344, 3825, 5720, 6489
HpaI GTTAAC 2 cut(s) 108, 973
HpaII CCGG 2 cut(s) 60, 122
HphI GGTGA 9 cut(s) 1009, 1289, 1846, 2471, 2708, 2792, 3749, 3800, 6497
HpyCH4IV ACGT 3 cut(s) 783, 2635, 6041
HpySE526I ACGT 3 cut(s) 783, 2635, 6041
Hsp92I GRCGYC 1 cut(s) 5952
HspAI GCGC 6 cut(s) 3653, 4263, 4402, 4559, 5359, 5952
KasI GGCGCC 1 cut(s) 5951
Kpn2I TCCGGA 2 cut(s) 59, 121
KpnI GGTACC 1 cut(s) 953
Ksp22I TGATCA 1 cut(s) 2347
KspAI GTTAAC 2 cut(s) 108, 973
LguI GCTCTTC 2 cut(s) 5349, 5445
LmnI GCTCC 9 cut(s) 525, 934, 1680, 2221, 3627, 3779, 4429, 5677, 6112
MaeII ACGT 3 cut(s) 783, 2635, 6041
MfeI CAATTG 2 cut(s) 3600, 5189
MflI RGATCY 7 cut(s) 13, 1073, 1691, 2150, 2414, 2449, 2710
MlsI TGGCCA 2 cut(s) 822, 4601
MluNI TGGCCA 2 cut(s) 822, 4601
Mly113I GGCGCC 1 cut(s) 5952
MlyI GAGTC 9 cut(s) 755, 1850, 2054, 3236, 3350, 3515, 4142, 5272, 6119
MmeI TCCRAC 9 cut(s) 1591, 1699, 2110, 3068, 3135, 5813, 5974, 6024, 6204
Mox20I TGGCCA 2 cut(s) 822, 4601
Mph1103I ATGCAT 3 cut(s) 1177, 1828, 5057
MroI TCCGGA 2 cut(s) 59, 121
MroXI GAANNNNTTC 2 cut(s) 620, 6620
MscI TGGCCA 2 cut(s) 822, 4601
Msp20I TGGCCA 2 cut(s) 822, 4601
MspA1I CMGCKG 1 cut(s) 1289
MspCI CTTAAG 1 cut(s) 4973
MspI CCGG 2 cut(s) 60, 122
MspR9I CCNGG 8 cut(s) 1832, 2777, 2830, 2859, 2919, 3038, 4836, 5889
MunI CAATTG 2 cut(s) 3600, 5189
Mva1269I GAATGC 3 cut(s) 1428, 4238, 5057
MvaI CCWGG 8 cut(s) 1832, 2777, 2830, 2859, 2919, 3038, 4836, 5889
MvnI CGCG 1 cut(s) 3653
NarI GGCGCC 1 cut(s) 5952
NcoI CCATGG 3 cut(s) 953, 3585, 5652
NdeI CATATG 3 cut(s) 2026, 3427, 5350
NmeAIII GCCGAG 3 cut(s) 2128, 3177, 4638
NmuCI GTSAC 8 cut(s) 906, 997, 2206, 2339, 3592, 3987, 4120, 5275
NsiI ATGCAT 3 cut(s) 1177, 1828, 5057
NspV TTCGAA 2 cut(s) 4607, 5669
OliI CACNNNNGTG 1 cut(s) 4389
PaeI GCATGC 1 cut(s) 520
PagI TCATGA 3 cut(s) 1498, 2532, 4843
PciI ACATGT 3 cut(s) 1135, 2316, 4088
PciSI GCTCTTC 2 cut(s) 5349, 5445
PcsI WCGNNNNNNNCGW 2 cut(s) 1574, 5228
PctI GAATGC 3 cut(s) 1428, 4238, 5057
PdmI GAANNNNTTC 2 cut(s) 620, 6620
PflMI CCANNNNNTGG 2 cut(s) 23, 2960
PfoI TCCNGGA 1 cut(s) 3036
PleI GAGTC 9 cut(s) 755, 1849, 2054, 3236, 3350, 3514, 4141, 5272, 6119
PluTI GGCGCC 1 cut(s) 5955
PpsI GAGTC 9 cut(s) 755, 1849, 2054, 3236, 3350, 3514, 4141, 5272, 6119
Ppu21I YACGTR 1 cut(s) 2636
PpuMI RGGWCCY 2 cut(s) 916, 3076
PscI ACATGT 3 cut(s) 1135, 2316, 4088
PshAI GACNNNNGTC 2 cut(s) 3505, 4503
PshBI ATTAAT 4 cut(s) 20, 1536, 2999, 4583
PsiI TTATAA 2 cut(s) 2435, 4310
Psp124BI GAGCTC 1 cut(s) 1936
Psp5II RGGWCCY 2 cut(s) 916, 3076
Psp6I CCWGG 8 cut(s) 1830, 2775, 2828, 2857, 2917, 3036, 4834, 5887
PspEI GGTNACC 1 cut(s) 997
PspGI CCWGG 8 cut(s) 1830, 2775, 2828, 2857, 2917, 3036, 4834, 5887
PspPPI RGGWCCY 2 cut(s) 916, 3076
PstI CTGCAG 1 cut(s) 1384
PstNI CAGNNNCTG 3 cut(s) 1040, 4320, 6062
PsuI RGATCY 7 cut(s) 13, 1073, 1691, 2150, 2414, 2449, 2710
PvuII CAGCTG 1 cut(s) 1289
SacI GAGCTC 1 cut(s) 1936
SalI GTCGAC 1 cut(s) 3766
SapI GCTCTTC 2 cut(s) 5349, 5445
ScaI AGTACT 1 cut(s) 5800
SchI GAGTC 9 cut(s) 755, 1850, 2054, 3236, 3350, 3515, 4142, 5272, 6119
ScrFI CCNGG 8 cut(s) 1832, 2777, 2830, 2859, 2919, 3038, 4836, 5889
SfcI CTRYAG 5 cut(s) 216, 1041, 1380, 3761, 4901
SfoI GGCGCC 1 cut(s) 5953
SfuI TTCGAA 2 cut(s) 4607, 5669
SinI GGWCC 8 cut(s) 916, 941, 2705, 3076, 3215, 4415, 4750, 5745
SmlI CTYRAG 7 cut(s) 549, 1985, 3472, 3503, 4672, 4973, 5399
SmoI CTYRAG 7 cut(s) 549, 1985, 3472, 3503, 4672, 4973, 5399
SnaBI TACGTA 1 cut(s) 2636
SpeI ACTAGT 1 cut(s) 1117
SphI GCATGC 1 cut(s) 520
SsiI CCGC 8 cut(s) 1754, 2173, 2482, 2624, 3651, 5503, 5864, 6315
SspDI GGCGCC 1 cut(s) 5951
SspI AATATT 2 cut(s) 5390, 6299
SstI GAGCTC 1 cut(s) 1936
StyD4I CCNGG 8 cut(s) 1830, 2775, 2828, 2857, 2917, 3036, 4834, 5887
StyI CCWWGG 6 cut(s) 186, 849, 953, 3585, 5196, 5652
TaiI ACGT 3 cut(s) 786, 2638, 6044
TaqII GACCGA 3 cut(s) 3746, 5096, 6158
TauI GCSGC 2 cut(s) 5866, 6318
TseFI GTSAC 8 cut(s) 906, 997, 2206, 2339, 3592, 3987, 4120, 5275
Tsp45I GTSAC 8 cut(s) 906, 997, 2206, 2339, 3592, 3987, 4120, 5275
TspGWI ACGGA 2 cut(s) 3031, 4880
Van91I CCANNNNNTGG 2 cut(s) 23, 2960
Vha464I CTTAAG 1 cut(s) 4973
VneI GTGCAC 1 cut(s) 5907
VpaK11BI GGWCC 8 cut(s) 916, 941, 2705, 3076, 3215, 4415, 4750, 5745
VspI ATTAAT 4 cut(s) 20, 1536, 2999, 4583
XbaI TCTAGA 2 cut(s) 2801, 4507
XmiI GTMKAC 5 cut(s) 219, 448, 3438, 3767, 5543
XmnI GAANNNNTTC 2 cut(s) 620, 6620
ZrmI AGTACT 1 cut(s) 5800
Zsp2I ATGCAT 3 cut(s) 1177, 1828, 5057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.