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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 206 |
| AccB1I | GGYRCC | 1 cut(s) | 206 |
| AciI | CCGC | 1 cut(s) | 356 |
| AclWI | GGATC | 1 cut(s) | 109 |
| AcsI | RAATTY | 1 cut(s) | 172 |
| AcuI | CTGAAG | 3 cut(s) | 99, 239, 321 |
| AfaI | GTAC | 1 cut(s) | 208 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 41, 350 |
| AjiI | CACGTC | 1 cut(s) | 251 |
| AluBI | AGCT | 1 cut(s) | 280 |
| AluI | AGCT | 1 cut(s) | 280 |
| AlwI | GGATC | 1 cut(s) | 109 |
| ApoI | RAATTY | 1 cut(s) | 172 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 244 |
| Asp718I | GGTACC | 1 cut(s) | 206 |
| AspLEI | GCGC | 1 cut(s) | 6 |
| BanI | GGYRCC | 1 cut(s) | 206 |
| BauI | CACGAG | 2 cut(s) | 66, 141 |
| BbsI | GAAGAC | 1 cut(s) | 245 |
| BccI | CCATC | 1 cut(s) | 179 |
| BclI | TGATCA | 3 cut(s) | 82, 157, 274 |
| BfmI | CTRYAG | 2 cut(s) | 213, 348 |
| BmgBI | CACGTC | 1 cut(s) | 251 |
| BmiI | GGNNCC | 1 cut(s) | 208 |
| BmrI | ACTGGG | 1 cut(s) | 240 |
| BmsI | GCATC | 1 cut(s) | 268 |
| BmuI | ACTGGG | 1 cut(s) | 240 |
| BpiI | GAAGAC | 1 cut(s) | 245 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 41, 350 |
| Bse1I | ACTGG | 3 cut(s) | 46, 235, 330 |
| Bse3DI | GCAATG | 1 cut(s) | 132 |
| BseGI | GGATG | 1 cut(s) | 24 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 41, 350 |
| BseMI | GCAATG | 1 cut(s) | 132 |
| BseNI | ACTGG | 3 cut(s) | 46, 235, 330 |
| BseRI | GAGGAG | 1 cut(s) | 385 |
| BshNI | GGYRCC | 1 cut(s) | 206 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 41, 350 |
| BsmI | GAATGC | 1 cut(s) | 383 |
| Bsp143I | GATC | 4 cut(s) | 82, 101, 157, 274 |
| BspACI | CCGC | 1 cut(s) | 356 |
| BspLI | GGNNCC | 1 cut(s) | 208 |
| BspPI | GGATC | 1 cut(s) | 109 |
| BspQI | GCTCTTC | 1 cut(s) | 297 |
| BspT107I | GGYRCC | 1 cut(s) | 206 |
| BsrDI | GCAATG | 1 cut(s) | 132 |
| BsrI | ACTGG | 3 cut(s) | 46, 235, 330 |
| BssMI | GATC | 4 cut(s) | 82, 101, 157, 274 |
| BssSI | CACGAG | 2 cut(s) | 66, 141 |
| Bst2BI | CACGAG | 2 cut(s) | 66, 141 |
| Bst6I | CTCTTC | 1 cut(s) | 297 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 348 |
| BstF5I | GGATG | 1 cut(s) | 24 |
| BstHHI | GCGC | 1 cut(s) | 6 |
| BstKTI | GATC | 4 cut(s) | 85, 104, 160, 277 |
| BstMBI | GATC | 4 cut(s) | 82, 101, 157, 274 |
| BstSFI | CTRYAG | 2 cut(s) | 213, 348 |
| BstV2I | GAAGAC | 1 cut(s) | 245 |
| BstX2I | RGATCY | 1 cut(s) | 101 |
| BstYI | RGATCY | 1 cut(s) | 101 |
| BtrI | CACGTC | 1 cut(s) | 251 |
| BtsCI | GGATG | 1 cut(s) | 24 |
| CfoI | GCGC | 1 cut(s) | 6 |
| Csp6I | GTAC | 1 cut(s) | 207 |
| CviAII | CATG | 2 cut(s) | 90, 165 |
| CviJI | RGCY | 5 cut(s) | 34, 88, 163, 280, 329 |
| CviKI_1 | RGCY | 5 cut(s) | 34, 88, 163, 280, 329 |
| CviQI | GTAC | 1 cut(s) | 207 |
| DpnI | GATC | 4 cut(s) | 84, 103, 159, 276 |
| DpnII | GATC | 4 cut(s) | 82, 101, 157, 274 |
| Eam1104I | CTCTTC | 1 cut(s) | 297 |
| EarI | CTCTTC | 1 cut(s) | 297 |
| Eco57I | CTGAAG | 3 cut(s) | 99, 239, 321 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 348 |
| FaeI | CATG | 2 cut(s) | 93, 168 |
| FaiI | YATR | 2 cut(s) | 91, 166 |
| FatI | CATG | 2 cut(s) | 89, 164 |
| FauI | CCCGC | 1 cut(s) | 349 |
| FbaI | TGATCA | 3 cut(s) | 82, 157, 274 |
| FokI | GGATG | 1 cut(s) | 11 |
| GlaI | GCGC | 1 cut(s) | 5 |
| HhaI | GCGC | 1 cut(s) | 6 |
| Hin1II | CATG | 2 cut(s) | 93, 168 |
| Hin6I | GCGC | 1 cut(s) | 4 |
| HinP1I | GCGC | 1 cut(s) | 4 |
| HinfI | GANTC | 1 cut(s) | 189 |
| Hpy166II | GTNNAC | 3 cut(s) | 59, 134, 248 |
| Hpy188I | TCNGA | 1 cut(s) | 239 |
| Hpy188III | TCNNGA | 1 cut(s) | 338 |
| Hpy8I | GTNNAC | 3 cut(s) | 59, 134, 248 |
| HpyCH4IV | ACGT | 1 cut(s) | 250 |
| HpyCH4V | TGCA | 5 cut(s) | 50, 73, 125, 148, 381 |
| HpySE526I | ACGT | 1 cut(s) | 250 |
| Hsp92II | CATG | 2 cut(s) | 93, 168 |
| HspAI | GCGC | 1 cut(s) | 4 |
| KpnI | GGTACC | 1 cut(s) | 210 |
| Ksp22I | TGATCA | 3 cut(s) | 82, 157, 274 |
| Kzo9I | GATC | 4 cut(s) | 82, 101, 157, 274 |
| LguI | GCTCTTC | 1 cut(s) | 297 |
| LpnPI | CCDG | 5 cut(s) | 27, 102, 216, 336, 343 |
| LweI | GCATC | 1 cut(s) | 268 |
| MaeII | ACGT | 1 cut(s) | 250 |
| MaeIII | GTNAC | 1 cut(s) | 292 |
| MalI | GATC | 4 cut(s) | 84, 103, 159, 276 |
| MboI | GATC | 4 cut(s) | 82, 101, 157, 274 |
| MboII | GAAGA | 3 cut(s) | 194, 245, 314 |
| MflI | RGATCY | 1 cut(s) | 101 |
| MluCI | AATT | 3 cut(s) | 172, 193, 240 |
| MlyI | GAGTC | 1 cut(s) | 183 |
| MnlI | CCTC | 2 cut(s) | 354, 363 |
| MroXI | GAANNNNTTC | 1 cut(s) | 244 |
| Mva1269I | GAATGC | 1 cut(s) | 383 |
| NdeII | GATC | 4 cut(s) | 82, 101, 157, 274 |
| NlaIII | CATG | 2 cut(s) | 93, 168 |
| NlaIV | GGNNCC | 1 cut(s) | 208 |
| PciSI | GCTCTTC | 1 cut(s) | 297 |
| PctI | GAATGC | 1 cut(s) | 383 |
| PdmI | GAANNNNTTC | 1 cut(s) | 244 |
| PleI | GAGTC | 1 cut(s) | 183 |
| PpsI | GAGTC | 1 cut(s) | 183 |
| PspN4I | GGNNCC | 1 cut(s) | 208 |
| PsuI | RGATCY | 1 cut(s) | 101 |
| RsaI | GTAC | 1 cut(s) | 208 |
| RsaNI | GTAC | 1 cut(s) | 207 |
| SapI | GCTCTTC | 1 cut(s) | 297 |
| Sau3AI | GATC | 4 cut(s) | 82, 101, 157, 274 |
| SchI | GAGTC | 1 cut(s) | 183 |
| SetI | ASST | 4 cut(s) | 121, 253, 282, 355 |
| SfaNI | GCATC | 1 cut(s) | 268 |
| SfcI | CTRYAG | 2 cut(s) | 213, 348 |
| Sse9I | AATT | 3 cut(s) | 172, 193, 240 |
| SsiI | CCGC | 1 cut(s) | 356 |
| TaiI | ACGT | 1 cut(s) | 253 |
| TasI | AATT | 3 cut(s) | 172, 193, 240 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 348 |
| XapI | RAATTY | 1 cut(s) | 172 |
| XmnI | GAANNNNTTC | 1 cut(s) | 244 |