RLG00000008155

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000582
Physical Location & Seq
Reverse (-)
26321135 .. 26324928
3794 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008155

Sequence Viewer

Length: 2739 bp
ATGAACGCACCTGCTTCTCTCTCCTCCGGCAGCCCATGGGGCCAGCAACCGCCGCCGCCGCCGCTTCTTGCCCTAAATCCCAATTTCTCTCTCCAAAACCCCCTCAATCTATACATGTACCTTTCAAACCTCGCCTCCTTCACACCCCACTACTATCCACCTTTCCCTCCCAATAATTTCCCAATACAAAACCCTAGCTTCGCCCCCAAGCACTTCTCCAATTCAGCATTTCATCCTCAGGCTCAGCCTTCGTCCCAGAACTCGAAAGAGCTGCTGGAGAGAATCGACCGCGCCGTGGAGAAGGCTCGCTCCGACCTTATTGCCGCCGGGGAAAGCGTCTCGGCGTGGAAGGTCTCGCAGTCTGCGATTCGGATACTCGAGGTTGATTGCTGGAGCTCTCTAGGGTTTCAAATGCAGCAGGTGCCGTCTCTGCACCGCCTCATGCTCACTCAAGGCAAGATAGATGCATTTATCCATTGTTTTGTTGGGGTTCGAAGAATTGCTTCATTGTATGATCTCGAAGTAGCAATCTGCGAGAATGAGGGCGTTGAACGGTTTGAAGAGCTGGGATTGGGCCCTTTGTTGCGAAATTCTCTTGTCGTGCATTATTTCTCAGTGAAGTGTGATGCAACTGAAGTGTTTAGGATAACGAGTGAGGAGATAATATCTTTACTTTCTGAGTTCATGGATGCTTGTAAACATACATATATAAGGGTTGAAGAATTTTTAGATTTCGTTGTAAAGAAGTGCGCAGTTGCAAGCAAGGAAGAGCTTGGAATACGAATTCACAGCTTGGGGATGCATATTTCAGCCATCCGGGTTGCTAGGGATTTAGAATCTGCTGCTCTGAAGAAATCTGGAGAGTTAATTGTTCAAAAACCAGATTCAAGATGTATGAAGCGACCAATGAGAATGCAGCAAGACGAACATTTGACCACCTCCATACATAAACAGTTTTGTGGCAAGCACATTAGGTTTGATTCAGAGGATGAATGTAGTGATGACAAAAATAATGACCATGTCAGAAGAAACCAGGTTAACTTATCTTCACAATCTGCTAAAAGTTCTAACAGAGTGGACAGTGATGATGGTTTGGTTGTCTCTCCATCACAATCCAAGGGTCCACTCAACCAGGTTAACATATCATCACAATCTGCTGAAAGATTTGACAGAGTGAACACTGATGATGGTTTGGTGGTCTCTCCATCACAATCCAAAGGTCCACTTCCCTCCTTGAAAATGCAGCAAGACAAAGATTTCAGTTCTATTTCTTGTCATGTTGATTCATTTTTCTCTGCACATAAGCAGTTTTGTGGCAAGCACGTTAGGTTTGATTTAGAGGATGAAGGTAGTGATGATGAAAATTATGATCATGTCAGAAGCAACCAGGTTAGCTTATCTTCACAATCTGCTAAAAGTTCTGACAGAGTGGACTGTGATGATGGTTTGGTTGTCTCTCCATCACAATCCAAGGGTCCACACAACCAGGTTAACTTATCATCACAATCTGCTGAAAATTTTGTCAGAGTGAACACTGATGATGGTTTGGTGGTCTCTCCATCACAATCCAAAGGTCCACTTCCCTCATTGAAAATGCAGCAAGACAAGGGTTTCAGTACCATTTCTTGTCATGTTGATTCATTTTTCTATGTAAATAAGCAGTTTTGTGGCAAGCACATTAGGTTTGATTTTGCAGAAAACTCGGAGGACGAATGTAGTGGTGATGATTTACATGATGAAGAAGATTATAATCATGACCAGGTCACATGCAGTGTGGTTAACTTCGCATCACAATCTGGTGGAAGGTCTGACCGTGTGAGCAGCTGTCCTTACCCATCTGCAATAGAAGAGATACAACGGCTTGGGTTGGACCAGCTTTCCCCAGATAGTGGCTGCCAAAAGCACAAAGGGCCTATTGACTCAGTTAAAAAGAAGAGAAAATCTGAAAATCTTCTAAGTGCAATCTCTGTGCCCCCCAAATTGTGTAAGACAGAAAAGGTAGAACAAGTCACTCGGCCAATAGAGAATGGCAGAGAAGCCAATACTGTGAGACATTTGGATGGAAGGAAAGCGGAAAGCTGGCCACCTGTTGATTGGAGAACTGCACCAAGGTATGACTATGCTTGTGCAAATGGCTTTAGGACACGGGCACCAATTTTACAGCCTAGAAATGACTTACAAAAGAAGGGGGATGATAATTATGATGGATTTGTTAAACGGACGGATAATATGATTCCTGTTACAGATACTCCTCAGTGGGGCACATCTAACTTTGGCAAGAGAGATCGTCTTAGTACTTGCACACCTGATGCAATACAAGCAAAGGAAACTGGGAGACTAGGAGAGCTTGCTGCTTTTAAATACTTTATTGAGAAAGCTGGCAAGTCAGCTGTGAAGTGGGTTAATGAGCATAGTGAAACAAGGCTACCGTATGACATAGTGGTAGGGGAGAATGAAGATAGTAAAGAGTTCATTGAAGTTAAAGCAACCAGAAATCCAACCAAAGACTGGTTTGAAATGTCAGTGAGACAGTTGCAATTTGCAGTTGAGCAAGGTGAAGCTTTCAGTATTGCGCATGTCATTTTATTAGATAATAATGTGGCGACAGTGAGAGTATATAACAATCTGGAGAAACTATTGATGAAGCATAACAACCAGGAGCTACAGGACCAGGATTGCAAGCTGAAGTTGGTTGTCTTGGTGCTTGAGCCGCAGAAGGAATTTACCATTGTGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

913

Amino Acids

102.3

Weight (kDa)

5.93

Isoelectric Point (pI)

56.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOV_C PF13020 781 - 866 4.4e-18 Protein NO VEIN, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1749
AarI CACCTGC 2 cut(s) 19, 409
Acc16I TGCGCA 2 cut(s) 751, 2574
Acc36I ACCTGC 2 cut(s) 19, 409
AccB1I GGYRCC 2 cut(s) 421, 2149
AccB7I CCANNNNNTGG 2 cut(s) 1889, 2508
AccII CGCG 1 cut(s) 291
AcoI YGGCCR 2 cut(s) 2015, 2081
AcsI RAATTY 5 cut(s) 589, 722, 783, 1516, 2720
AcuI CTGAAG 3 cut(s) 654, 869, 2705
AfaI GTAC 3 cut(s) 119, 1618, 2296
AfiI CCNNNNNNNGG 4 cut(s) 295, 1889, 2258, 2508
AflIII ACRYGT 1 cut(s) 114
AjnI CCWGG 7 cut(s) 1032, 1131, 1386, 1485, 1758, 2655, 2670
Alw21I GWGCWC 1 cut(s) 398
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 5 cut(s) 40, 574, 1910, 2015, 2081
ApaI GGGCCC 1 cut(s) 578
ApoI RAATTY 5 cut(s) 589, 722, 783, 1516, 2720
Asp700I GAANNNNTTC 2 cut(s) 502, 1950
AspLEI GCGC 3 cut(s) 293, 752, 2575
AsuC2I CCSGG 2 cut(s) 328, 818
AsuHPI GGTGA 2 cut(s) 1733, 2567
AsuII TTCGAA 1 cut(s) 493
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 6 cut(s) 1121, 1220, 1475, 1574, 1870, 2668
AxyI CCTNAGG 1 cut(s) 237
BaeGI GKGCMC 4 cut(s) 578, 1974, 2152, 2264
BalI TGGCCA 1 cut(s) 2083
BanI GGYRCC 2 cut(s) 421, 2149
BanII GRGCYC 2 cut(s) 398, 578
Bbv12I GWGCWC 1 cut(s) 398
BceAI ACGGC 3 cut(s) 278, 409, 1874
BcgI CGANNNNNNTGC 6 cut(s) 253, 287, 302, 336, 1683, 1717
BciT130I CCWGG 7 cut(s) 1034, 1133, 1388, 1487, 1760, 2657, 2672
BciVI GTATCC 1 cut(s) 366
BclI TGATCA 1 cut(s) 1369
BcnI CCSGG 2 cut(s) 328, 818
BfaI CTAG 5 cut(s) 195, 401, 825, 2166, 2339
BfmI CTRYAG 1 cut(s) 2663
BfuAI ACCTGC 2 cut(s) 19, 409
BfuI GTATCC 1 cut(s) 366
BglI GCCNNNNNGGC 1 cut(s) 39
BlpI GCTNAGC 1 cut(s) 243
BmcAI AGTACT 1 cut(s) 2296
Bme1390I CCNGG 9 cut(s) 328, 818, 1034, 1133, 1388, 1487, 1760, 2657, 2672
Bme18I GGWCC 6 cut(s) 1121, 1220, 1475, 1574, 1870, 2668
BmeT110I CYCGRG 1 cut(s) 377
BmiI GGNNCC 6 cut(s) 41, 423, 576, 1122, 1476, 2151
BmrFI CCNGG 9 cut(s) 328, 818, 1034, 1133, 1388, 1487, 1760, 2657, 2672
BmrI ACTGGG 1 cut(s) 2340
BmsI GCATC 6 cut(s) 454, 616, 679, 789, 1796, 2299
BmuI ACTGGG 1 cut(s) 2340
BpmI CTGGAG 4 cut(s) 296, 412, 879, 2648
Bpu1102I GCTNAGC 1 cut(s) 243
Bpu14I TTCGAA 1 cut(s) 493
BpuEI CTTGAG 2 cut(s) 435, 2726
BpuMI CCSGG 2 cut(s) 328, 818
BsaBI GATNNNNATC 1 cut(s) 1749
BsaI GGTCTC 3 cut(s) 358, 1204, 1558
BsaJI CCNNGG 6 cut(s) 35, 294, 327, 1116, 1470, 2108
BsaXI ACNNNNNCTCC 4 cut(s) 119, 149, 2233, 2263
Bsc4I CCNNNNNNNGG 4 cut(s) 295, 1889, 2258, 2508
Bse1I ACTGG 2 cut(s) 2335, 2513
Bse21I CCTNAGG 1 cut(s) 237
Bse8I GATNNNNATC 1 cut(s) 1749
BseBI CCWGG 7 cut(s) 1034, 1133, 1388, 1487, 1760, 2657, 2672
BseDI CCNNGG 6 cut(s) 35, 294, 327, 1116, 1470, 2108
BseGI GGATG 8 cut(s) 232, 694, 804, 813, 994, 1348, 2065, 2197
BseJI GATNNNNATC 1 cut(s) 1749
BseLI CCNNNNNNNGG 4 cut(s) 295, 1889, 2258, 2508
BseMII CTCAG 6 cut(s) 251, 257, 627, 669, 1935, 2267
BseNI ACTGG 2 cut(s) 2335, 2513
BseRI GAGGAG 3 cut(s) 13, 671, 2241
BseSI GKGCMC 4 cut(s) 578, 1974, 2152, 2264
BseYI CCCAGC 1 cut(s) 565
BsgI GTGCAG 3 cut(s) 416, 1281, 2088
Bsh1236I CGCG 1 cut(s) 291
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 5 cut(s) 42, 576, 1912, 2017, 2083
BshNI GGYRCC 2 cut(s) 421, 2149
BsiEI CGRYCG 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 398
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 3 cut(s) 27, 327, 817
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 4 cut(s) 295, 1889, 2258, 2508
BsmBI CGTCTC 2 cut(s) 343, 432
BsmFI GGGAC 1 cut(s) 238
BsmI GAATGC 1 cut(s) 918
BsnI GGCC 5 cut(s) 42, 576, 1912, 2017, 2083
Bso31I GGTCTC 3 cut(s) 358, 1204, 1558
BsoBI CYCGRG 1 cut(s) 377
Bsp119I TTCGAA 1 cut(s) 493
Bsp120I GGGCCC 1 cut(s) 574
Bsp1286I GDGCHC 5 cut(s) 398, 578, 1974, 2152, 2264
Bsp143I GATC 3 cut(s) 514, 1369, 2284
Bsp1720I GCTNAGC 1 cut(s) 243
Bsp19I CCATGG 1 cut(s) 35
BspANI GGCC 5 cut(s) 42, 576, 1912, 2017, 2083
BspCNI CTCAG 6 cut(s) 250, 256, 626, 670, 1934, 2266
BspFNI CGCG 1 cut(s) 291
BspHI TCATGA 1 cut(s) 1753
BspLI GGNNCC 6 cut(s) 41, 423, 576, 1122, 1476, 2151
BspMI ACCTGC 2 cut(s) 19, 409
BspQI GCTCTTC 2 cut(s) 555, 762
BspT104I TTCGAA 1 cut(s) 493
BspT107I GGYRCC 2 cut(s) 421, 2149
BspTNI GGTCTC 3 cut(s) 358, 1204, 1558
BsrI ACTGG 2 cut(s) 2335, 2513
BssECI CCNNGG 6 cut(s) 35, 294, 327, 1116, 1470, 2108
BssMI GATC 3 cut(s) 514, 1369, 2284
BssT1I CCWWGG 4 cut(s) 35, 1116, 1470, 2108
Bst2UI CCWGG 7 cut(s) 1034, 1133, 1388, 1487, 1760, 2657, 2672
Bst4CI ACNGT 9 cut(s) 555, 954, 1082, 1436, 1814, 2047, 2430, 2532, 2608
Bst6I CTCTTC 4 cut(s) 555, 762, 1842, 1928
BstAPI GCANNNNNTGC 1 cut(s) 421
BstBI TTCGAA 1 cut(s) 493
BstDEI CTNAG 8 cut(s) 237, 243, 613, 678, 1921, 1955, 2253, 2291
BstDSI CCRYGG 2 cut(s) 35, 294
BstF5I GGATG 8 cut(s) 232, 694, 804, 813, 994, 1348, 2065, 2197
BstFNI CGCG 1 cut(s) 291
BstHHI GCGC 3 cut(s) 293, 752, 2575
BstKTI GATC 3 cut(s) 517, 1372, 2287
BstMBI GATC 3 cut(s) 514, 1369, 2284
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 9 cut(s) 39, 52, 58, 61, 421, 430, 1909, 2318, 2710
BstNI CCWGG 7 cut(s) 1034, 1133, 1388, 1487, 1760, 2657, 2672
BstNSI RCATGY 3 cut(s) 118, 1770, 2579
BstSCI CCNGG 9 cut(s) 326, 816, 1032, 1131, 1386, 1485, 1758, 2655, 2670
BstSFI CTRYAG 1 cut(s) 2663
BstSLI GKGCMC 4 cut(s) 578, 1974, 2152, 2264
BstUI CGCG 1 cut(s) 291
Bsu36I CCTNAGG 1 cut(s) 237
BsuI GTATCC 1 cut(s) 366
BsuRI GGCC 5 cut(s) 42, 576, 1912, 2017, 2083
BtgI CCRYGG 2 cut(s) 35, 294
BtsCI GGATG 8 cut(s) 232, 694, 804, 813, 994, 1348, 2065, 2197
BtsI GCAGTG 1 cut(s) 1777
BtsIMutI CAGTG 8 cut(s) 621, 1087, 1179, 1533, 1777, 2261, 2529, 2613
BveI ACCTGC 2 cut(s) 19, 409
CciI TCATGA 1 cut(s) 1753
CfoI GCGC 3 cut(s) 293, 752, 2575
CseI GACGC 1 cut(s) 325
CsiI ACCWGGT 5 cut(s) 1032, 1131, 1386, 1485, 1758
Csp6I GTAC 3 cut(s) 118, 1617, 2295
CviQI GTAC 3 cut(s) 118, 1617, 2295
DdeI CTNAG 8 cut(s) 237, 243, 613, 678, 1921, 1955, 2253, 2291
DpnI GATC 3 cut(s) 516, 1371, 2286
DpnII GATC 3 cut(s) 514, 1369, 2284
DraI TTTAAA 1 cut(s) 2359
EaeI YGGCCR 2 cut(s) 2015, 2081
Eam1104I CTCTTC 4 cut(s) 555, 762, 1842, 1928
EarI CTCTTC 4 cut(s) 555, 762, 1842, 1928
Ecl136II GAGCTC 1 cut(s) 396
Eco130I CCWWGG 4 cut(s) 35, 1116, 1470, 2108
Eco24I GRGCYC 2 cut(s) 398, 578
Eco31I GGTCTC 3 cut(s) 358, 1204, 1558
Eco47I GGWCC 6 cut(s) 1121, 1220, 1475, 1574, 1870, 2668
Eco53kI GAGCTC 1 cut(s) 396
Eco57I CTGAAG 3 cut(s) 654, 869, 2705
Eco81I CCTNAGG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 396
EcoO109I RGGNCCY 2 cut(s) 575, 1910
EcoRI GAATTC 1 cut(s) 783
EcoRII CCWGG 7 cut(s) 1032, 1131, 1386, 1485, 1758, 2655, 2670
EcoT14I CCWWGG 4 cut(s) 35, 1116, 1470, 2108
EcoT22I ATGCAT 2 cut(s) 469, 804
EcoT38I GRGCYC 2 cut(s) 398, 578
ErhI CCWWGG 4 cut(s) 35, 1116, 1470, 2108
Esp3I CGTCTC 2 cut(s) 343, 432
FalI AAGNNNNNCTT 6 cut(s) 487, 519, 1209, 1241, 1563, 1595
FaqI GGGAC 1 cut(s) 238
FbaI TGATCA 1 cut(s) 1369
FokI GGATG 8 cut(s) 219, 701, 800, 811, 1001, 1355, 2072, 2204
FriOI GRGCYC 2 cut(s) 398, 578
FspBI CTAG 5 cut(s) 195, 401, 825, 2166, 2339
FspI TGCGCA 2 cut(s) 751, 2574
GlaI GCGC 3 cut(s) 292, 751, 2574
GsaI CCCAGC 1 cut(s) 569
GsuI CTGGAG 4 cut(s) 296, 412, 879, 2648
HaeIII GGCC 5 cut(s) 42, 576, 1912, 2017, 2083
HapII CCGG 3 cut(s) 27, 327, 817
HgaI GACGC 1 cut(s) 325
HhaI GCGC 3 cut(s) 293, 752, 2575
Hin6I GCGC 3 cut(s) 291, 750, 2573
HinP1I GCGC 3 cut(s) 291, 750, 2573
HincII GTYRAC 4 cut(s) 1039, 1138, 1492, 1780
HindII GTYRAC 4 cut(s) 1039, 1138, 1492, 1780
HindIII AAGCTT 1 cut(s) 2559
HinfI GANTC 9 cut(s) 282, 367, 836, 884, 980, 1283, 1637, 1919, 2233
HpaI GTTAAC 4 cut(s) 1039, 1138, 1492, 1780
HpaII CCGG 3 cut(s) 27, 327, 817
HphI GGTGA 2 cut(s) 1733, 2567
Hpy188III TCNNGA 6 cut(s) 518, 858, 888, 1754, 2627, 2736
HpyAV CCTTC 9 cut(s) 148, 258, 295, 343, 1340, 1797, 2058, 2179, 2710
HpyCH4III ACNGT 9 cut(s) 555, 954, 1082, 1436, 1814, 2047, 2430, 2532, 2608
HpyCH4IV ACGT 1 cut(s) 1323
HpyF10VI GCNNNNNNNGC 9 cut(s) 39, 52, 58, 61, 421, 430, 1909, 2318, 2710
HpyF3I CTNAG 8 cut(s) 237, 243, 613, 678, 1921, 1955, 2253, 2291
HpySE526I ACGT 1 cut(s) 1323
HspAI GCGC 3 cut(s) 291, 750, 2573
Ksp22I TGATCA 1 cut(s) 1369
KspAI GTTAAC 4 cut(s) 1039, 1138, 1492, 1780
Kzo9I GATC 3 cut(s) 514, 1369, 2284
LguI GCTCTTC 2 cut(s) 555, 762
LmnI GCTCC 3 cut(s) 314, 393, 2659
LweI GCATC 6 cut(s) 454, 616, 679, 789, 1796, 2299
MabI ACCWGGT 5 cut(s) 1032, 1131, 1386, 1485, 1758
MaeI CTAG 5 cut(s) 195, 401, 825, 2166, 2339
MaeII ACGT 1 cut(s) 1323
MaeIII GTNAC 3 cut(s) 1762, 2008, 2239
MalI GATC 3 cut(s) 516, 1371, 2286
MboI GATC 3 cut(s) 514, 1369, 2284
MhlI GDGCHC 5 cut(s) 398, 578, 1974, 2152, 2264
MlsI TGGCCA 1 cut(s) 2083
MluNI TGGCCA 1 cut(s) 2083
MlyI GAGTC 1 cut(s) 1913
MmeI TCCRAC 3 cut(s) 336, 1848, 2522
Mox20I TGGCCA 1 cut(s) 2083
Mph1103I ATGCAT 2 cut(s) 469, 804
MroXI GAANNNNTTC 2 cut(s) 502, 1950
MscI TGGCCA 1 cut(s) 2083
MslI CAYNNNNRTG 1 cut(s) 2058
Msp20I TGGCCA 1 cut(s) 2083
MspA1I CMGCKG 2 cut(s) 1824, 2390
MspI CCGG 3 cut(s) 27, 327, 817
MspR9I CCNGG 9 cut(s) 328, 818, 1034, 1133, 1388, 1487, 1760, 2657, 2672
Mva1269I GAATGC 1 cut(s) 918
MvaI CCWGG 7 cut(s) 1034, 1133, 1388, 1487, 1760, 2657, 2672
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 9 cut(s) 39, 52, 58, 61, 421, 430, 1909, 2318, 2710
NciI CCSGG 2 cut(s) 328, 818
NcoI CCATGG 1 cut(s) 35
NdeII GATC 3 cut(s) 514, 1369, 2284
NlaIV GGNNCC 6 cut(s) 41, 423, 576, 1122, 1476, 2151
NmeAIII GCCGAG 2 cut(s) 320, 1993
NmuCI GTSAC 2 cut(s) 1762, 2008
NsbI TGCGCA 2 cut(s) 751, 2574
NsiI ATGCAT 2 cut(s) 469, 804
NspI RCATGY 3 cut(s) 118, 1770, 2579
NspV TTCGAA 1 cut(s) 493
PaeR7I CTCGAG 1 cut(s) 377
PagI TCATGA 1 cut(s) 1753
PaqCI CACCTGC 2 cut(s) 19, 409
PciI ACATGT 1 cut(s) 114
PciSI GCTCTTC 2 cut(s) 555, 762
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 362
PctI GAATGC 1 cut(s) 918
PdmI GAANNNNTTC 2 cut(s) 502, 1950
PfeI GAWTC 8 cut(s) 282, 367, 836, 884, 980, 1283, 1637, 2233
PflFI GACNNNGTC 2 cut(s) 1019, 1760
PflMI CCANNNNNTGG 2 cut(s) 1889, 2508
PleI GAGTC 1 cut(s) 1913
PpsI GAGTC 1 cut(s) 1913
PscI ACATGT 1 cut(s) 114
PsiI TTATAA 1 cut(s) 1749
Psp124BI GAGCTC 1 cut(s) 398
Psp6I CCWGG 7 cut(s) 1032, 1131, 1386, 1485, 1758, 2655, 2670
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 7 cut(s) 1032, 1131, 1386, 1485, 1758, 2655, 2670
PspN4I GGNNCC 6 cut(s) 41, 423, 576, 1122, 1476, 2151
PspOMI GGGCCC 1 cut(s) 574
PspXI VCTCGAGB 1 cut(s) 377
PsyI GACNNNGTC 2 cut(s) 1019, 1760
PvuII CAGCTG 2 cut(s) 1824, 2390
RsaI GTAC 3 cut(s) 119, 1618, 2296
RsaNI GTAC 3 cut(s) 118, 1617, 2295
RseI CAYNNNNRTG 1 cut(s) 2058
SacI GAGCTC 1 cut(s) 398
SapI GCTCTTC 2 cut(s) 555, 762
Sau3AI GATC 3 cut(s) 514, 1369, 2284
ScaI AGTACT 1 cut(s) 2296
SchI GAGTC 1 cut(s) 1913
ScrFI CCNGG 9 cut(s) 328, 818, 1034, 1133, 1388, 1487, 1760, 2657, 2672
SduI GDGCHC 5 cut(s) 398, 578, 1974, 2152, 2264
SexAI ACCWGGT 5 cut(s) 1032, 1131, 1386, 1485, 1758
SfaNI GCATC 6 cut(s) 454, 616, 679, 789, 1796, 2299
SfcI CTRYAG 1 cut(s) 2663
Sfr274I CTCGAG 1 cut(s) 377
SfuI TTCGAA 1 cut(s) 493
SinI GGWCC 6 cut(s) 1121, 1220, 1475, 1574, 1870, 2668
SlaI CTCGAG 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 2058
SmlI CTYRAG 3 cut(s) 377, 450, 2705
SmoI CTYRAG 3 cut(s) 377, 450, 2705
SspMI CTAG 5 cut(s) 195, 401, 825, 2166, 2339
SstI GAGCTC 1 cut(s) 398
StyD4I CCNGG 9 cut(s) 326, 816, 1032, 1131, 1386, 1485, 1758, 2655, 2670
StyI CCWWGG 4 cut(s) 35, 1116, 1470, 2108
TaaI ACNGT 9 cut(s) 555, 954, 1082, 1436, 1814, 2047, 2430, 2532, 2608
TaiI ACGT 1 cut(s) 1326
TaqI TCGA 5 cut(s) 263, 285, 378, 493, 519
TatI WGTACW 1 cut(s) 2294
TauI GCSGC 6 cut(s) 55, 58, 61, 64, 326, 2713
TfiI GAWTC 8 cut(s) 282, 367, 836, 884, 980, 1283, 1637, 2233
TscAI CASTG 8 cut(s) 621, 1087, 1186, 1540, 1777, 2261, 2529, 2613
TseFI GTSAC 2 cut(s) 1762, 2008
Tsp45I GTSAC 2 cut(s) 1762, 2008
TspGWI ACGGA 2 cut(s) 2233, 2237
TspRI CASTG 8 cut(s) 621, 1087, 1186, 1540, 1777, 2261, 2529, 2613
Tth111I GACNNNGTC 2 cut(s) 1019, 1760
Van91I CCANNNNNTGG 2 cut(s) 1889, 2508
VpaK11BI GGWCC 6 cut(s) 1121, 1220, 1475, 1574, 1870, 2668
XapI RAATTY 5 cut(s) 589, 722, 783, 1516, 2720
XceI RCATGY 3 cut(s) 118, 1770, 2579
XcmI CCANNNNNNNNNTGG 3 cut(s) 482, 2091, 2505
XhoI CTCGAG 1 cut(s) 377
XmnI GAANNNNTTC 2 cut(s) 502, 1950
XspI CTAG 5 cut(s) 195, 401, 825, 2166, 2339
ZrmI AGTACT 1 cut(s) 2296
Zsp2I ATGCAT 2 cut(s) 469, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.