Rh4AG185300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000582
Physical Location & Seq
Forward (+)
46103495 .. 46209200
105706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG185300.1

Sequence Viewer

Length: 8541 bp
ATGGCCGGTTACCCTCATCACTTCCGCCCCTCCAGTGGGGACTGGAACTGGGCACACCCACCTCCTCACCTCTCCCACCCACAATTTCCTTACCCCAATTTTCCTCCCCAAAACCCTACCAATTTAAACATCTATCCCCCTCAACCTTCCGCCTTTCCACCACCGCAACCTCAACCGCCGCCGCCGCAACCGTCAAACCCTAATTTCCCCCTCCAACACCCTATAGACGTCAATATCTTTCCCGCCAACCACTATGGACACTCCTCATTTTCCAATTACTACCCAACCCCAACCCCAAGCTCTAATTTCTCTCTGCAAAACCCTAACCCCACTCCACCCTTCCCCCCTCAGAGCTTCTCCCGTAACCCTAACGAGGCGCCCGACCCGCCGAACCTCCCGAATTCGGCTAGGCATTTGCCTGAGGACCCGAGAGAGGTGCTCGAGAAGATCGACCGTGCGGTGGAGAAGCTTCGCGGCGAGCTTGTCGCCGTCGGAAAGAACGTCTCGGCCTGGAAGGTGTTACAGTCTGCGCTTCTGATGCTCAATGTTGATGCCGAGACTTGTTTAGGCCTCCAAATTGAGCAAGTGCCGTCCCTTCACCGCCTCATGGTCACTGAAGGAAGGATAAATGCATTTATCCATTGCTTTGTCGGGGTTCGAACAATTACATCTTTGTATGATTTGGAAGTTGCAATCTGCAAGAATGAGGATATTGAGCAGTTTGAAGAGCTTGAATTGGGTCCTTTGTTGCGACACCCACTAGTTTCGCAATATTTCTCAGTGGATTCTGAAACAACTGAAGTCCTTAAGATATCGAGTGAGGAGATACTACATTTACTCAGTGGGTATTTAAAGATGCCGAAAGTTGAGGAAAATTCTGTTGAGGGGTTCTTGGACTTCATTGTTACGAAGCGACCTGTTGCAGGCAAGGAAAAGCTTGGAATCCGAATATTTAGTTTGGGGGTGCATACTTCTGCTATGCGTGAAGCCAAGGCTTCGGAGCGTGCAGCTTTCTGGAAATTATTACGGGAATTAACACACAAGTCTCCCAAAATATCTAGGTCGAAGGGACCAAGTTTATCTATGGTGAAAAAGCGGCTAGATGAACATTTTCACGCCATTTTGCAGCAATCTGGACTGTGTTCTGCTGTACAAAAGAATGGAAAGGAAGATACACTTGTTTCTTCAAGATCAAAGGATGAAGGTATTAATGATTATTTACATGATGACGAAGGTGATGACGATGGTACTGGTGGTCATATTGACTCATTAAAATCCCTGAAAAGTTCTGACAGAGAGGGTAAGTGTTCTTACTTGTCTGAAGTGGAGGAGAGGTCTTCGCTCATGTCAAACCACCCTTCTCCAAGCCAAAAACATAATGAGAGTAATGGGTCAGTTAAAAAGAAAAGAAAATACGAAAATCTAAGCTGCCCTATCTCTGTTCCCTTAAAGTTGCTCAAGAGAGAGAAGGTAGAGCAAGATGCTGTACCTAAAGAGAGTGACATTGAAACTAAGGAGGTCAGCGAGGTGCTTAAACTTGATCTATCAATTGCCAATAATCTTTTGAGGATGTTCATTACAACTTGGAAGCAGGCATGTAGGGAGCAGCCTGTGGCTGAGGTACTTTGGGCCATGCTTCGCTCTTGTAAAATTAAAAAAAAAAAAGCAAAGAGAATCATGGCAATGTTTTCGACATACCCTTTTGTCGGATTACTCAATGTTGCTGTCTCGTCTGTCAAATGTGGAACGTGGGATAATATGTTTGATACGTTCCAAACCATTAGTCCACCTGAGTTGGCTAACACTGTAACTGATAATTATCCTGAACGTGTCGAACAAAGTATAAAGGATGCACCACCAGTCAGCACTGAACATGCTACCGAACATGGACAAACCTCTTGCTGCCTGAAGCCGAATAATCAGGATCGTCAACGTCGTCGGAGGAAGATCCAAAGGAGTCATAAGAAGTTGGAGCCTGAAAACGGGCACATCTGCTACCTTTGTCATCAAGAGGGACACATGAAGAGAAGATGCCCTCTGCGGCTGCAAACTGAAAAATCATCTTATGAGCCAGAGAGTCCTCAACAATTGGCTCAGTACTCTATTTCCGGAGAAGAGGTGGAGGAGAAAGAAAGTGCTGTGCTGGACGACTCCACAGGTGTTTCAGTTAAAGAGATAATCAGGAAAGTTACTACATATTTTCAGACTGATCACGGAATGTCTAGCAATGGTGAATCACTTCCGGAGAAGATATTTATTTACTTCAGAAAGCTTTGCAATTGTGAGCTCTGGTTGACTAAAGAATTTTCTATCAAGGAATTTAGATCCCTTGGTTATGGGGAATTTATGATGTTTTTAGAAAAGCATGCCCGTCTTCTGCCACAGGAGATGTGCAAGTTCTTGAAAGATGACAGCAATGGAAAGTTTCCTTTGGAGGTTTGCATGCTTCCGCATTATTTAGTTGTTTTGGTATCTCAAGCTTTAAACAATTTATGGGAGGACCAAAAGATTACCAAACTGAACATTTCTTCTCTACTTGGGAAGCAGTTCCCGGCCCTTAGCTTCCAAACCATTGAAAATGGTTCAGTAGAAGACTTTAAAAGTATAGTAGGAAAGCATAAAGACACTGCGATTTCCAAATGTGTACTATTTTCAATGACAATATGCGGAACTAGTTATACTTTAGAATCATTGGAAAACTGTAGTAAGAATGTTGTATTGAAAAGCAGTTTGGTGAGTGTTAACAGTGGCCAGAAAATAAGCAATCAATCTGTTGCATCCAAGGAGGCGATTGCAGTCCTACTTAGGGCACCCATGATGTCAGATTTGAATTTATGGTCACATTGGGACCTCTTATTTGCTCCCTCTCTTGGTCCGCTTGTACCGTGGTTATTGAATGAAGTCAACACTGATGAATTACTCTGTTTGGTAACCAAAGATGGCAAAGTTATTCGGTTAGATCATTCAGCTACTGTAGATTCATTCTCAAAAGCTGCTCTTCAAGGATCCTCTTTTCAAACAGCAGTGAAAATGTTATCTCTCTTTTCACTGGTTGGGGGAGAAAAACATGTCCCCGTGTCCCTTTTGAAATTCCATGTCCAGCGTGCATTTGAAGTTATTTTCAAGACCTATCTAGATGGTATGGAAGTATATGACAATAGGAGTTCTCTTAATCAAGGAACAGTTTTCTGTGGACAGAAAATGGTTGTTGAAATTGCTGCTGGTAAATGTAGTAACGTACAGAGAGATGTAACGCAAACAAAAATTGCTGAGTCTGTTATATCACATTTTTTTCTTGATTGCCTGTGGTATCTACCTGCAGAGTTTCGCAGTTTTGCTGCTGATGTGTTACTTTCTGGGATGCGGTCTGTTTTTAAGCATGCAGCTTCAGCCATTTTGGATGAATGCATCCAAATAGAGCAGCGTCTCATACTTCATGAAGTTGGCTTATCTCTTGGTATAGTGGAGTGGATTAGTGATTACCATGTATTGAGTTCCTTTGATTCTACTGATTTGTTTATGTCTGAGGCTTCTGGTCTCAATGCTGACAGAGATCAAATGGGTTCAGACTCAAAATATATGCAAAATGTGTCAAAAAAGTTGGCTGCTTCTGAACAGAGTGTGGCTGCATGTGTTAGGGCAGATGAGCATAAAAGAGGATGTGCTGATGTTAGCCCGATGGTTGATGGTGTAGGAGTTTCTGAGGCTGGGATTGGCATTGGTTACAGACAACATTCTACTAATATTAATGAACATAAAGATGCTTTCCAGGTTATTGAGTCAATCAGGAGAGATGAATTCGGTTTGGATTCATGCCTATTGACCTTCGAAAGTATTGTCTTAAAGAAACAGCATGCTCGTTTAGGGAGAGCTCTGCACTGTCTTTCACAGGAATTATATTCTCAAGATTCTCATTTTCTTCTTGAGCTGGTTCAAAATGCTGATGATAATACTTATCCAACAAATATAGAACCAACTCTAGCATTCATTCTTCAAGAGTCAGGTATTGTTGTTCTGAATAATGAGCAAGGCTTCTCTGCCCAGAACATAAGAGCACTTTGTGATGTTGGAAATTCAACCAAGCAAGGATCCAATGCTGGATATATAGGGCAAAAGGGCATCGGTTTCAAATCAGTATTTCGTGTCACAGATGCTCCAGAAATTCATTCGAATGGATTTCATATCAAGTTTGATACAAATGAGGGTCAGATTGGTTTAATTTTGCCGACAGTTGTACCTCCTTGCGATCTTGAGTTGTTTAGACAGCTGATATCTGGTGACATTGATGAACCAGATTGTTACTGCTGGAAAACTTGTATTGTTCTTCCTTTCAGATCGAAAGTATCAGATGGAACTGTCTTGAACCACATAATGAATATGTTTTCAGATCTTCATCCATCTTTACTACTCTTTCTTCACCGCCTCAAGTGTATCCAGTTTAGAAACCTAATCGATGATTCACTTACTGTCATGAGAAAAGAAATTGTCGGAGACGGTATTGTTAAGGTTTCACATGGGAATACGAAGATGACATGGTTTGTAGTATCTCAGAAATTGCACTCAGATAACCTTCGTAGTGATGTGCAAACAACAGAAATCTCAATTGCCTTTACATTGAAGGAATCGGATAAGGCCATTTATTGTCCAGATATAGGCCAACAGCTTGTTTTTGCATTTCTTCCTCTTAGGACTTATGGACTGAAATTTATTGTTCAGGGTGATTTTGTTCTTCCTTCATCCAGAGAGGAAGTGGATGGAGATAGTCCCTGGAACCAATGGCTGTTGTCAGAATTACCTGGTTTATTTGTCGATGCAGAGAAATCATTTTGTTCTCTTCCATGTTTCAAAGAGAATCCTGGGACTGCTGTGGCAGCTTATATGTCCTTTGTTCCACTTGTTGGGGAAGTGCATGGTTTCTTTTCTTCTCTTCCTCGATTAATTATTTCTAAATTACGGATGTCAAATTGCTTACTCCAGGAAGGAGAAAATAAAGAATGGGTTTCTCCTTGCAAGGTCCTAAGAGGTTGGACTAAACAGGCTCGTTCACTTCTCCCTGATGGTCTACTGCATGAGCATCTTGGCCTTGGATTCTTGGATAAAGATATAGTTCTGCCTGATCCACTTGCAAGAGCTCTAGGTATTCCAGAGTATGGGCCCACAGTTCTGCTTCAGGTAATGGCTTCTTTATGTGGCAAGCAGGATGGCCTCAAATCAATGGGAATGGGTTGGATGGCCTCTTTGCTTAGTGAACTCTATGCAATGTCATTCAAATCTTCTGTTGAAACTTCCTTTGATTCTGGAACAGAAATGGTATTCCTAGAAAAACTTCAAAAAATTCCATTCATACCTCTTTCAGATGGTACATATGGTTCAGTGGTTGAAGGCCCAATTTGGCTACAGTTTGATGCTGTAGGGACTGGTTTTGGGGATCATCATGGGCTTGAATCTTTTCCAAAGTTATATGCTAAACTTCGGATTGTAAGTCCGGAGCTCTATGCATCATCTGCTGATATGCCATCCATGTATGTGACACCAATTGACAGAGTCATTAGCATGCTTCATAGAATTGGTGTCCAACGGTTGTCCGCACATGAGATTCTCAAGGCTCACATCTTGCCAGTTGTATCTGATGATAGAATTACAGTTAGGGATAAAGATTTGATGACTGATTATATTTGCTTTGCAATGATTCACTTACAGTCGAGCTGCTCTGATTGTCATGCTGAAAGGGAATACATCATATCAGAACTACAAAATAAAGCTTATATTCTAACTAATAATGGCTTTAAACGACCTGCTGAGGCATCAATTCATTTCAGTACAGAATTTGGAAATCCAGTGGACATAAGTAGGTTGATTGATAGGGTAAATCTGACATGGGATGAGGTTGATATCTCCTATTTGAAGCATCCAGTCGCTAAATCATTTACAAATGGACTGATGAAGTGGAGAGAATTTTTCCAGAAAATTGGCATTGTGGATTTTGTGAAGGTAGTTCAAGTTGAGAAGGTTTTCACCGAGTTGTCTGAGGCTCTATTTAATACTCTGACTTGGGACCAGCACACTATTTCCCATGGGTTGAATGCCACAGATTGGGAATCGCCTGAGTTAGTCCATTTATTATCCATATTGTCCAGAGATGGCAACAGAAAAGGTTGTGAGTATCTCTTAGAGGTTCTCGATAAATTATGGGATGATTGCTATAGTGACAGAGCTACAGGGTACTGCTCTTCTAAGTCTGTTGCAGATAGAAAGCCCTTCAAATCTTCATTTATGAGCACCATCTGCGATGTGCAATGGGTAGTATCAGGAATGGATGATAAACTTCACTATCCCAAGGATCTATATCACGACAGTGTTGCAGTGCGGTCAATTCTTGGTGGATCTGCCCCATTTTTTGTTCCAAAGGCTAGCAGTGAAAAATTTGTATCTGATATTGGTTTTAAGACAAGAGTCTCTCTTCATGATGCTCTGGAGATGTTGAAACTATGGAGATGTGAGAATCCGTTCTGGGCCAGCCTAGCACAAATGTCCAAATTTTACTCATTAATCTGGAATGAAATGGCTTCTTCAAAGAAGACAACTGCAGATGAGTTCTGTTTGGAACCTTTCGTATTTGTTCCATATGAATCTAGCTTCCGGCATGAGGATGTGGTGTCTGGTACATTTCTGTCCCTTGAAGAAGTATACTGGGATGATTCTACTTTCTTTGTGGACCAAATCAAGGAGATCCATCATCAGTGCAGCTCAACGGTTGGTAATCATGGCCCCATAAACAAGATTATGAGTAATTTCTACCCGGCCCTTCATGACTTCTTTGTTGATATATGTGGAGTACATGAGATCCCTCCTCTTCGTAGCTACCTTCAGATTTTGCTACAGTTTTCAAATGCTGTTTTGCCTTCACAAGCAGCTAATGCTGCTTTTCAAGTTTTTCAGAAGTGGGCTGATGGGCTCCAGTCAGGAATGAGTGCTGAGGATATTGTTTACTTGAAAGATTCTCTTACAAGGATGGAATGTAATGTGCTTCCTACTGTACAGGATAAGTGGGTATCTCTACATCCTTCCTATGGTCTCCTGTGCTGGTGTGATGATAAGAAGTTGAAAAAGCAGTTTATGCATATGGATGGACTTGATTTTCTTTACTTCGGTGGACCAAGTAATGATGATGAAGAGATCCTAAGCACAAAAATGTCTGTCCTCTTGCGAACCTTAGGGATTCCTGCACTTTCAGAGGTTGTGACTCGTGAGGCGATGTTTAATGGTCCGACAGATTCTAGCTATAAAGCTGCATTGCTGGATTGGGCTCTGCCATATGCGCAGCGCTACTTACATAGCTTACATCCTGATAAATATTCTCAACACAAGCAGTCTGGATTCCATATAGTAAATCGTTTGCGAGTAGTAGAAGTTCAAAAGCTGTCCTACCGCAATGTTATAAAGATCGCTGGAAGTGAATCCAAGAAGCAAATTGAATGTAGTTGTGTTCTGCAGGATCACGATCTGTATACAACCCAGGAATCAGATTCTCACGCATTATTTATGGAGCTTTCTCGTTTGTTTTTTGATGGAAAACCAGAATTACACTTGGCAAATTTCCTTCATATGATCACAACAATGGCTGAATCAGGTTCAACTGAGGAGCAGATAGACTTTTTTATCTTCAATAGCCAAAAGGTGCCAAAGCTTCCTGATGGAGAATCTGTTTGGTCCCTGTCACATGTGCATAATGATAGGTTACCCCAGCCAAGTGACTGGCGCTTTGTTGGTTGGAAAGCCCCACTAAGCTTTGGTTATGCAAATCTTTTCAAGGCTCAGGCACAATTTGCACAGCTTACCAGTGTCTCACAAATTGAAATGGATAATGATTCTGAAACCCATGTCAGACAAACAGTTGAATCAACTCCCATTTCAGTCGACATTAAATGGACAATTGGTGAGGGTACAGCAACAACATCTGCACCTTTAGTGCTGCCAAACTCCAATGATTTGCAAGAACACTTTGATGATGAGACTGATATGGATACTGATTTCAATCCTAATAATCTAGATTTTGTTGTTGACCCTCGCATTTTGGGTTCTTCTGATTGTAGCAAAGTAGACCATCCTAGATATGGCTCTAGTGGAAGAGATGCAATGCGCGGAAGAGATGCAATGCTTACCGGGAGGTTAGGTGAGGTTGTTGCTTTTAAATACTTAATTGCAAAAGCTGGCAAGTCAGTTGTGAGGTGGGTTAATGAAAGAATTGAAACTGGGCTACCCTATGACATAGTTGTAGGTGAGAAGGAAGATAGTCTAGAGTTCATTGAGGTTAAAGCCACCCAACAACGAAAAAAGGATTGGTTCCACATATCAATGAGGGAGTGGCAATTTGCAGCGGAAAAAGGTGAAGCCTTTAGCATTTTGCACGTCCTCTTATTGGGTAATAATGCTGCAAGAGTCTCAGTTTACAAGAATCCTGTAAAATTGTGTCATCTGGGCAAGCTACAGTTGCATATTTCGATGCCTAGGCAGGAGAAGGAATTATTTCTTGTGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3883

Protein Analysis

2846

Amino Acids

320.42

Weight (kDa)

5.88

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SACS PF25794 1304 - 1400 7.1e-09 Sacsin domain
NOV_C PF13020 2725 - 2811 2.7e-19 Protein NO VEIN, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000582)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08300 AT1G08300 AT1G08300 AT4G13750
fragaria_vesca FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_2g13620 FvH4_4g13620 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13640 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660 FvH4_4g13660
malus_domestica MD04G1012800.v1.1 MD04G1012900.v1.1 MD04G1013100.v1.1 MD04G1013300.v1.1
prunus_persica Prupe.1G159000_v2.0.a1 Prupe.1G159100_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G159200_v2.0.a1 Prupe.1G358100_v2.0.a1
pyrus_communis pycom04g01020 pycom04g01030
rosa_chinensis RchiOBHm_Chr4g0414341 RchiOBHm_Chr4g0414351 RchiOBHm_Chr6g0275261 RchiOBHm_Chr7g0213881 RchiOBHm_Chr7g0213891
rosa_laevigata RLG00000008153 RLG00000008155 RLG00000013477
rosa_multiflora Rmu_co8510163.1_g000001 Rmu_sc0004087.1_g000027 Rmu_sc0005196.1_g000014 Rmu_sc0005558.1_g000005 Rmu_sc0005558.1_g000006 Rmu_sc0008100.1_g000005 Rmu_sc0008560.1_g000005
rosa_roxburghii Rroxscaffold_5G00358210 Rroxscaffold_5G00358220 Rroxscaffold_7G00187920 Rroxscaffold_7G00187930 Rroxscaffold_7G00193450
rosa_rugosa Rorug04G0127100 Rorug04G0127200 Rorug04G0127300 Rorug04G0127400 Rorug06G0090600
rosa_samantha Rh4AG185200 Rh4AG185300 Rh4AG185500 Rh4BG184700 Rh4BG184800 Rh4CG198000 Rh4CG198100 Rh4DG182400 Rh4DG182600 Rh6AG204100 Rh6BG207000 Rh6CG209600 Rh6DG200000 Rh7AG277400 Rh7CG296400 Rh7CG296500
rosa_wichuraiana Rw4G015850 Rw4G015860 Rw6G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 7439
AatII GACGTC 1 cut(s) 231
Acc16I TGCGCA 1 cut(s) 7320
Acc36I ACCTGC 2 cut(s) 3325, 5803
AccB1I GGYRCC 3 cut(s) 376, 2809, 7678
AccB7I CCANNNNNTGG 3 cut(s) 4898, 5150, 6093
AccI GTMKAC 5 cut(s) 5062, 6687, 7508, 7917, 8100
AccII CGCG 2 cut(s) 474, 8142
AccIII TCCGGA 3 cut(s) 2108, 2242, 5485
AcoI YGGCCR 2 cut(s) 3, 2749
AcuI CTGAAG 8 cut(s) 636, 819, 1341, 1928, 2248, 3372, 5153, 6851
AcyI GRCGYC 2 cut(s) 228, 377
AdeI CACNNNGTG 1 cut(s) 4061
AfeI AGCGCT 1 cut(s) 7325
AflII CTTAAG 1 cut(s) 806
AflIII ACRYGT 3 cut(s) 1828, 3067, 7720
AhlI ACTAGT 2 cut(s) 760, 2672
AjiI CACGTC 1 cut(s) 8410
AjnI CCWGG 7 cut(s) 509, 3768, 4766, 4795, 4855, 4974, 7515
AjuI GAANNNNNNNTTGG 4 cut(s) 2714, 2746, 8323, 8355
AleI CACNNNNGTG 1 cut(s) 6354
Alw21I GWGCWC 7 cut(s) 441, 2289, 3874, 4057, 5134, 5493, 6281
AlwNI CAGNNNCTG 1 cut(s) 2972
Ama87I CYCGRG 2 cut(s) 427, 440
Aor13HI TCCGGA 3 cut(s) 2108, 2242, 5485
Aor51HI AGCGCT 1 cut(s) 7325
ApaI GGGCCC 1 cut(s) 5157
ArsI GACNNNNNNTTYG 8 cut(s) 1768, 1800, 2840, 2872, 3822, 3854, 7745, 7777
AseI ATTAAT 4 cut(s) 1209, 3747, 4937, 6548
Asp700I GAANNNNTTC 8 cut(s) 1110, 2238, 2546, 5325, 5448, 6011, 6506, 8526
AspA2I CCTAGG 1 cut(s) 8507
AspLEI GCGC 6 cut(s) 379, 532, 7321, 7326, 7761, 8142
AsuC2I CCSGG 3 cut(s) 2552, 6800, 8164
AsuII TTCGAA 3 cut(s) 658, 3828, 4169
AsuNHI GCTAGC 1 cut(s) 6410
AvaI CYCGRG 2 cut(s) 427, 440
AvrII CCTAGG 1 cut(s) 8507
AxyI CCTNAGG 2 cut(s) 420, 7213
BaeGI GKGCMC 4 cut(s) 55, 1989, 2812, 5157
BaeI ACNNNNGTAYC 4 cut(s) 1164, 1197, 4218, 4251
BalI TGGCCA 1 cut(s) 2751
BamHI GGATCC 2 cut(s) 3005, 4088
BanI GGYRCC 3 cut(s) 376, 2809, 7678
BanII GRGCYC 7 cut(s) 2289, 3874, 5134, 5157, 5493, 6957, 7309
BauI CACGAG 1 cut(s) 7245
BbsI GAAGAC 4 cut(s) 1329, 2366, 2598, 6584
Bbv12I GWGCWC 7 cut(s) 441, 2289, 3874, 4057, 5134, 5493, 6281
BbvCI CCTCAGC 3 cut(s) 1617, 5799, 6975
BceAI ACGGC 2 cut(s) 473, 574
BcgI CGANNNNNNTGC 6 cut(s) 418, 452, 1671, 1705, 6341, 6375
BciT130I CCWGG 7 cut(s) 511, 3770, 4768, 4797, 4857, 4976, 7517
BciVI GTATCC 2 cut(s) 4442, 8017
BclI TGATCA 2 cut(s) 2209, 7608
BcnI CCSGG 3 cut(s) 2552, 6800, 8164
BcuI ACTAGT 2 cut(s) 760, 2672
BfoI RGCGCY 3 cut(s) 380, 7327, 7762
BfrI CTTAAG 1 cut(s) 806
BfuAI ACCTGC 2 cut(s) 3325, 5803
BfuI GTATCC 2 cut(s) 4442, 8017
BglII AGATCT 1 cut(s) 4387
BlnI CCTAGG 1 cut(s) 8507
BmcAI AGTACT 1 cut(s) 2099
BmeT110I CYCGRG 2 cut(s) 427, 440
BmgBI CACGTC 1 cut(s) 8410
BmrI ACTGGG 3 cut(s) 58, 6700, 8262
BmtI GCTAGC 1 cut(s) 6414
BmuI ACTGGG 3 cut(s) 58, 6700, 8262
BoxI GACNNNNGTC 1 cut(s) 6452
BpiI GAAGAC 4 cut(s) 1329, 2366, 2598, 6584
BplI GAGNNNNNCTC 2 cut(s) 423, 455
BpmI CTGGAG 5 cut(s) 16, 4140, 4958, 6494, 6941
Bpu10I CCTNAGC 6 cut(s) 1617, 2558, 5799, 6975, 7181, 7815
Bpu14I TTCGAA 3 cut(s) 658, 3828, 4169
BpuEI CTTGAG 7 cut(s) 1442, 2460, 3888, 3944, 4271, 4409, 5585
BpuMI CCSGG 3 cut(s) 2552, 6800, 8164
Bsa29I ATCGAT 1 cut(s) 4452
BsaBI GATNNNNATC 5 cut(s) 1818, 4392, 6345, 7500, 8034
BsaHI GRCGYC 2 cut(s) 228, 377
BsaI GGTCTC 2 cut(s) 3542, 7079
BsaWI WCCGGW 3 cut(s) 2108, 2242, 5485
Bse118I RCCGGY 1 cut(s) 5
Bse21I CCTNAGG 2 cut(s) 420, 7213
Bse8I GATNNNNATC 5 cut(s) 1818, 4392, 6345, 7500, 8034
BseAI TCCGGA 3 cut(s) 2108, 2242, 5485
BseBI CCWGG 7 cut(s) 511, 3770, 4768, 4797, 4857, 4976, 7517
BseCI ATCGAT 1 cut(s) 4452
BseJI GATNNNNATC 5 cut(s) 1818, 4392, 6345, 7500, 8034
BseRI GAGGAG 7 cut(s) 54, 253, 836, 1343, 2138, 6840, 7655
BseSI GKGCMC 4 cut(s) 55, 1989, 2812, 5157
BseYI CCCAGC 2 cut(s) 3707, 7743
BsgI GTGCAG 5 cut(s) 1026, 3860, 6763, 7209, 7944
Bsh1236I CGCG 2 cut(s) 474, 8142
Bsh1285I CGRYCG 1 cut(s) 454
BshNI GGYRCC 3 cut(s) 376, 2809, 7678
BshVI ATCGAT 1 cut(s) 4452
BsiEI CGRYCG 1 cut(s) 454
BsiHKAI GWGCWC 7 cut(s) 441, 2289, 3874, 4057, 5134, 5493, 6281
BsiHKCI CYCGRG 2 cut(s) 427, 440
BsiSI CCGG 8 cut(s) 6, 2109, 2243, 2552, 5486, 6640, 6800, 8163
BsmBI CGTCTC 3 cut(s) 508, 3431, 4485
BsmI GAATGC 3 cut(s) 3410, 3983, 6088
Bso31I GGTCTC 2 cut(s) 3542, 7079
BsoBI CYCGRG 2 cut(s) 427, 440
Bsp119I TTCGAA 3 cut(s) 658, 3828, 4169
Bsp120I GGGCCC 1 cut(s) 5153
Bsp13I TCCGGA 3 cut(s) 2108, 2242, 5485
Bsp1407I TGTACA 2 cut(s) 1150, 7036
Bsp19I CCATGG 1 cut(s) 6073
BspDI ATCGAT 1 cut(s) 4452
BspEI TCCGGA 3 cut(s) 2108, 2242, 5485
BspFNI CGCG 2 cut(s) 474, 8142
BspHI TCATGA 4 cut(s) 3436, 4470, 6463, 6808
BspMAI CTGCAG 3 cut(s) 3322, 6589, 7494
BspMI ACCTGC 2 cut(s) 3325, 5803
BspOI GCTAGC 1 cut(s) 6414
BspQI GCTCTTC 3 cut(s) 720, 3003, 6235
BspT104I TTCGAA 3 cut(s) 658, 3828, 4169
BspT107I GGYRCC 3 cut(s) 376, 2809, 7678
BspTI CTTAAG 1 cut(s) 806
BspTNI GGTCTC 2 cut(s) 3542, 7079
BsrFI RCCGGY 1 cut(s) 5
BsrGI TGTACA 2 cut(s) 1150, 7036
BssAI RCCGGY 1 cut(s) 5
BssNAI GTATAC 2 cut(s) 6688, 7509
BssNI GRCGYC 2 cut(s) 228, 377
BssSI CACGAG 1 cut(s) 7245
BssT1I CCWWGG 7 cut(s) 990, 2329, 2781, 5083, 6073, 6336, 8507
Bst1107I GTATAC 2 cut(s) 6688, 7509
Bst2BI CACGAG 1 cut(s) 7245
Bst2UI CCWGG 7 cut(s) 511, 3770, 4768, 4797, 4857, 4976, 7517
BstACI GRCGYC 2 cut(s) 228, 377
BstAFI CTTAAG 1 cut(s) 806
BstAPI GCANNNNNTGC 5 cut(s) 5504, 6285, 6917, 7117, 7826
BstAUI TGTACA 2 cut(s) 1150, 7036
BstBI TTCGAA 3 cut(s) 658, 3828, 4169
BstDSI CCRYGG 2 cut(s) 2885, 6073
BstEII GGTNACC 3 cut(s) 8, 2929, 7737
BstENI CCTNNNNNAGG 1 cut(s) 921
BstFNI CGCG 2 cut(s) 474, 8142
BstH2I RGCGCY 3 cut(s) 380, 7327, 7762
BstHHI GCGC 6 cut(s) 379, 532, 7321, 7326, 7761, 8142
BstMCI CGRYCG 1 cut(s) 454
BstNI CCWGG 7 cut(s) 511, 3770, 4768, 4797, 4857, 4976, 7517
BstPAI GACNNNNGTC 1 cut(s) 6452
BstPI GGTNACC 3 cut(s) 8, 2929, 7737
BstSLI GKGCMC 4 cut(s) 55, 1989, 2812, 5157
BstUI CGCG 2 cut(s) 474, 8142
BstV2I GAAGAC 4 cut(s) 1329, 2366, 2598, 6584
BstXI CCANNNNNNTGG 2 cut(s) 5969, 7755
BstZ17I GTATAC 2 cut(s) 6688, 7509
Bsu15I ATCGAT 1 cut(s) 4452
Bsu36I CCTNAGG 2 cut(s) 420, 7213
BsuI GTATCC 2 cut(s) 4442, 8017
BsuTUI ATCGAT 1 cut(s) 4452
BtgI CCRYGG 2 cut(s) 2885, 6073
BtgZI GCGATG 2 cut(s) 6303, 7268
BtrI CACGTC 1 cut(s) 8410
BtsI GCAGTG 4 cut(s) 2625, 3030, 6369, 6421
BveI ACCTGC 2 cut(s) 3325, 5803
CaiI CAGNNNCTG 1 cut(s) 2972
CciI TCATGA 4 cut(s) 3436, 4470, 6463, 6808
CfoI GCGC 6 cut(s) 379, 532, 7321, 7326, 7761, 8142
Cfr10I RCCGGY 1 cut(s) 5
ClaI ATCGAT 1 cut(s) 4452
CseI GACGC 1 cut(s) 3413
CsiI ACCWGGT 1 cut(s) 4795
CspCI CAANNNNNGTGG 2 cut(s) 1776, 1811
DinI GGCGCC 1 cut(s) 378
DraI TTTAAA 6 cut(s) 126, 852, 2484, 2599, 5788, 8191
DraIII CACNNNGTG 1 cut(s) 4061
EaeI YGGCCR 2 cut(s) 3, 2749
EciI GGCGGA 2 cut(s) 14, 139
Ecl136II GAGCTC 4 cut(s) 2287, 3872, 5132, 5491
Eco130I CCWWGG 7 cut(s) 990, 2329, 2781, 5083, 6073, 6336, 8507
Eco147I AGGCCT 1 cut(s) 570
Eco24I GRGCYC 7 cut(s) 2289, 3874, 5134, 5157, 5493, 6957, 7309
Eco31I GGTCTC 2 cut(s) 3542, 7079
Eco32I GATATC 3 cut(s) 813, 4272, 5893
Eco47III AGCGCT 1 cut(s) 7325
Eco53kI GAGCTC 4 cut(s) 2287, 3872, 5132, 5491
Eco57I CTGAAG 8 cut(s) 636, 819, 1341, 1928, 2248, 3372, 5153, 6851
Eco81I CCTNAGG 2 cut(s) 420, 7213
Eco88I CYCGRG 2 cut(s) 427, 440
Eco91I GGTNACC 3 cut(s) 8, 2929, 7737
EcoICRI GAGCTC 4 cut(s) 2287, 3872, 5132, 5491
EcoNI CCTNNNNNAGG 1 cut(s) 921
EcoO109I RGGNCCY 4 cut(s) 424, 740, 2848, 5014
EcoO65I GGTNACC 3 cut(s) 8, 2929, 7737
EcoRI GAATTC 2 cut(s) 400, 3797
EcoRII CCWGG 7 cut(s) 509, 3768, 4766, 4795, 4855, 4974, 7515
EcoRV GATATC 3 cut(s) 813, 4272, 5893
EcoT14I CCWWGG 7 cut(s) 990, 2329, 2781, 5083, 6073, 6336, 8507
EcoT22I ATGCAT 4 cut(s) 634, 3410, 5500, 7122
EcoT38I GRGCYC 7 cut(s) 2289, 3874, 5134, 5157, 5493, 6957, 7309
EgeI GGCGCC 1 cut(s) 378
EheI GGCGCC 1 cut(s) 378
ErhI CCWWGG 7 cut(s) 990, 2329, 2781, 5083, 6073, 6336, 8507
Esp3I CGTCTC 3 cut(s) 508, 3431, 4485
FalI AAGNNNNNCTT 6 cut(s) 1161, 1193, 2982, 3014, 6987, 7019
FauI CCCGC 2 cut(s) 250, 393
FauNDI CATATG 5 cut(s) 5365, 6625, 7122, 7315, 7605
FbaI TGATCA 2 cut(s) 2209, 7608
FblI GTMKAC 5 cut(s) 5062, 6687, 7508, 7917, 8100
FriOI GRGCYC 7 cut(s) 2289, 3874, 5134, 5157, 5493, 6957, 7309
FspI TGCGCA 1 cut(s) 7320
GlaI GCGC 6 cut(s) 378, 531, 7320, 7325, 7760, 8141
GsaI CCCAGC 2 cut(s) 3711, 7747
GsuI CTGGAG 5 cut(s) 16, 4140, 4958, 6494, 6941
HaeII RGCGCY 3 cut(s) 380, 7327, 7762
HapII CCGG 8 cut(s) 6, 2109, 2243, 2552, 5486, 6640, 6800, 8163
HgaI GACGC 1 cut(s) 3413
HhaI GCGC 6 cut(s) 379, 532, 7321, 7326, 7761, 8142
Hin1I GRCGYC 2 cut(s) 228, 377
Hin6I GCGC 6 cut(s) 377, 530, 7319, 7324, 7759, 8140
HinP1I GCGC 6 cut(s) 377, 530, 7319, 7324, 7759, 8140
HincII GTYRAC 6 cut(s) 1931, 2295, 2743, 2905, 7918, 8062
HindII GTYRAC 6 cut(s) 1931, 2295, 2743, 2905, 7918, 8062
HindIII AAGCTT 7 cut(s) 467, 935, 2270, 2478, 5760, 7685, 7786
HpaI GTTAAC 1 cut(s) 2743
HpaII CCGG 8 cut(s) 6, 2109, 2243, 2552, 5486, 6640, 6800, 8163
Hpy99I CGWCG 3 cut(s) 494, 1938, 1941
HpyCH4IV ACGT 8 cut(s) 228, 501, 1748, 1769, 1828, 1933, 3237, 8409
HpySE526I ACGT 8 cut(s) 228, 501, 1748, 1769, 1828, 1933, 3237, 8409
Hsp92I GRCGYC 2 cut(s) 228, 377
HspAI GCGC 6 cut(s) 377, 530, 7319, 7324, 7759, 8140
KasI GGCGCC 1 cut(s) 376
Kpn2I TCCGGA 3 cut(s) 2108, 2242, 5485
Ksp22I TGATCA 2 cut(s) 2209, 7608
KspAI GTTAAC 1 cut(s) 2743
LguI GCTCTTC 3 cut(s) 720, 3003, 6235
LmnI GCTCC 9 cut(s) 1000, 1603, 1972, 2866, 4159, 5488, 6960, 7546, 7642
MabI ACCWGGT 1 cut(s) 4795
MaeII ACGT 8 cut(s) 228, 501, 1748, 1769, 1828, 1933, 3237, 8409
MfeI CAATTG 6 cut(s) 1548, 2087, 2278, 4602, 5535, 7932
MlsI TGGCCA 1 cut(s) 2751
MluNI TGGCCA 1 cut(s) 2751
Mly113I GGCGCC 1 cut(s) 377
Mox20I TGGCCA 1 cut(s) 2751
Mph1103I ATGCAT 4 cut(s) 634, 3410, 5500, 7122
MroI TCCGGA 3 cut(s) 2108, 2242, 5485
MroXI GAANNNNTTC 8 cut(s) 1110, 2238, 2546, 5325, 5448, 6011, 6506, 8526
MscI TGGCCA 1 cut(s) 2751
Msp20I TGGCCA 1 cut(s) 2751
MspA1I CMGCKG 2 cut(s) 4267, 8378
MspCI CTTAAG 1 cut(s) 806
MspI CCGG 8 cut(s) 6, 2109, 2243, 2552, 5486, 6640, 6800, 8163
MteI GCGCNGCGC 1 cut(s) 7322
MunI CAATTG 6 cut(s) 1548, 2087, 2278, 4602, 5535, 7932
Mva1269I GAATGC 3 cut(s) 3410, 3983, 6088
MvaI CCWGG 7 cut(s) 511, 3770, 4768, 4797, 4857, 4976, 7517
MvnI CGCG 2 cut(s) 474, 8142
NarI GGCGCC 1 cut(s) 377
NciI CCSGG 3 cut(s) 2552, 6800, 8164
NcoI CCATGG 1 cut(s) 6073
NdeI CATATG 5 cut(s) 5365, 6625, 7122, 7315, 7605
NheI GCTAGC 1 cut(s) 6410
NmeAIII GCCGAG 2 cut(s) 485, 580
NsbI TGCGCA 1 cut(s) 7320
NsiI ATGCAT 4 cut(s) 634, 3410, 5500, 7122
NspV TTCGAA 3 cut(s) 658, 3828, 4169
OliI CACNNNNGTG 1 cut(s) 6354
PaeI GCATGC 5 cut(s) 2369, 2446, 3383, 3857, 5557
PaeR7I CTCGAG 1 cut(s) 440
PagI TCATGA 4 cut(s) 3436, 4470, 6463, 6808
PceI AGGCCT 1 cut(s) 570
PciI ACATGT 2 cut(s) 3067, 7720
PciSI GCTCTTC 3 cut(s) 720, 3003, 6235
PcsI WCGNNNNNNNCGW 4 cut(s) 378, 447, 498, 1933
PctI GAATGC 3 cut(s) 3410, 3983, 6088
PdmI GAANNNNTTC 8 cut(s) 1110, 2238, 2546, 5325, 5448, 6011, 6506, 8526
PflFI GACNNNGTC 1 cut(s) 5543
PflMI CCANNNNNTGG 3 cut(s) 4898, 5150, 6093
PfoI TCCNGGA 1 cut(s) 4974
PluTI GGCGCC 1 cut(s) 380
PpuMI RGGWCCY 4 cut(s) 424, 740, 2848, 5014
PscI ACATGT 2 cut(s) 3067, 7720
PshAI GACNNNNGTC 1 cut(s) 6452
PshBI ATTAAT 4 cut(s) 1209, 3747, 4937, 6548
PsiI TTATAA 1 cut(s) 7439
Psp124BI GAGCTC 4 cut(s) 2289, 3874, 5134, 5493
Psp5II RGGWCCY 4 cut(s) 424, 740, 2848, 5014
Psp6I CCWGG 7 cut(s) 509, 3768, 4766, 4795, 4855, 4974, 7515
PspEI GGTNACC 3 cut(s) 8, 2929, 7737
PspFI CCCAGC 2 cut(s) 3707, 7743
PspGI CCWGG 7 cut(s) 509, 3768, 4766, 4795, 4855, 4974, 7515
PspOMI GGGCCC 1 cut(s) 5153
PspPPI RGGWCCY 4 cut(s) 424, 740, 2848, 5014
PsrI GAACNNNNNNTAC 6 cut(s) 2662, 2694, 5353, 5385, 7395, 7427
PstI CTGCAG 3 cut(s) 3322, 6589, 7494
PstNI CAGNNNCTG 1 cut(s) 2972
PsyI GACNNNGTC 1 cut(s) 5543
PvuII CAGCTG 1 cut(s) 4267
SacI GAGCTC 4 cut(s) 2289, 3874, 5134, 5493
SalI GTCGAC 1 cut(s) 7916
SapI GCTCTTC 3 cut(s) 720, 3003, 6235
ScaI AGTACT 1 cut(s) 2099
SexAI ACCWGGT 1 cut(s) 4795
SfoI GGCGCC 1 cut(s) 378
Sfr274I CTCGAG 1 cut(s) 440
SfuI TTCGAA 3 cut(s) 658, 3828, 4169
SlaI CTCGAG 1 cut(s) 440
SmlI CTYRAG 9 cut(s) 440, 806, 1457, 2475, 3903, 3923, 4250, 4424, 5600
SmoI CTYRAG 9 cut(s) 440, 806, 1457, 2475, 3903, 3923, 4250, 4424, 5600
SpeI ACTAGT 2 cut(s) 760, 2672
SphI GCATGC 5 cut(s) 2369, 2446, 3383, 3857, 5557
SseBI AGGCCT 1 cut(s) 570
SspDI GGCGCC 1 cut(s) 376
SspI AATATT 4 cut(s) 773, 951, 3745, 7355
SstI GAGCTC 4 cut(s) 2289, 3874, 5134, 5493
StuI AGGCCT 1 cut(s) 570
StyI CCWWGG 7 cut(s) 990, 2329, 2781, 5083, 6073, 6336, 8507
TaiI ACGT 8 cut(s) 231, 504, 1751, 1772, 1831, 1936, 3240, 8412
TatI WGTACW 6 cut(s) 1150, 2097, 2644, 5819, 6835, 7036
TauI GCSGC 6 cut(s) 181, 184, 187, 477, 1099, 2044
TspGWI ACGGA 3 cut(s) 2229, 4969, 6495
Tth111I GACNNNGTC 1 cut(s) 5543
Van91I CCANNNNNTGG 3 cut(s) 4898, 5150, 6093
Vha464I CTTAAG 1 cut(s) 806
VspI ATTAAT 4 cut(s) 1209, 3747, 4937, 6548
XagI CCTNNNNNAGG 1 cut(s) 921
XbaI TCTAGA 3 cut(s) 3133, 8047, 8296
XcmI CCANNNNNNNNNTGG 2 cut(s) 4747, 8111
XhoI CTCGAG 1 cut(s) 440
XmaJI CCTAGG 1 cut(s) 8507
XmiI GTMKAC 5 cut(s) 5062, 6687, 7508, 7917, 8100
XmnI GAANNNNTTC 8 cut(s) 1110, 2238, 2546, 5325, 5448, 6011, 6506, 8526
ZraI GACGTC 1 cut(s) 229
ZrmI AGTACT 1 cut(s) 2099
Zsp2I ATGCAT 4 cut(s) 634, 3410, 5500, 7122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.