Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0000664

Physical Location & Seq

Genomic Coordinates Reverse (-)

7038216 .. 7042057

Gene Structure 3842 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1060500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1653 bp

ATGGCCGCCAAACGGAGTATGTGCGTGTGCTTCGCCGTGGTTTTCATCTCGTCGGTTCTGGTTTCAGGCGCCGGAGCATTTACCGACCCGCTTGACCTCACGGCTCTTCATGATTTTTACCGGGCCCTTAACAACCCACCGGTGCTGAAGGGGTGGCGATCGGATGGTGGGGATCCTTGTGAAGAGTCATGGGATGGAGTTTCATGTTCTGGTTCCTCTGTGATATACCTCAAACTTCGTAGTCTGAACCTTTCTGGATATATTCCTGAGGAGCTCTATAATCTGTTCAATTTAAAGCAACTGAATCTGAGCCACAATTTGTTTTCGGGACCTATTGGCAATGTATTTACCAGCCTACAGAATTTAAGAGAACTTGATTTGTCATACAATAACTTCACCGGAGATCTACCAAGTTCTTTTGGTTCCATGACGAATCTCACTGGATTGTTCCTGCAGAATAACAAATTCACTGGATCAGTTGCTTTCCTAGCTGAGCTTCCACTAACTGATCTGAACATACAAGATAATTATTTCAGCGGCATCATTCCAAATCATTTTCAGTCCATACCAAATTTATGGATTGGGGGTAATAGTTTTCATGTAGGGGCTAACTCTCCACCTTGGGATTTTCCTCTGGAAACCCAGGCAAGTTCTGTCGTACAAAACACTCCTGCGCCCCCAGCCATTCATTCAAATTCCATTGAGAAGAATCCCCCTACGAAATTAGGTGGATACAAGAAGAAAAGGATGAGCCCTGGAGGAATAGCATTTTTGGTTGGTGGAGTCACTCTAGTGGCATCATGTGTGGCACTTTACATAGCAGTTCGCATTAATCAATTGCGTGCACAGAAGCTTATAACCTTGGGGGGAAGTAACAGCTCAAGGAATTCTCTCTCAATAAGAACCACTGCCAGAGATGGTTCTTCTATTGCAGAAGAAAGCCCATCAATGCTTCCTTTCAGGTCACCCATGCTACTTCCAAGGCGACTGCCTCCAGTTTACCATAGCAGACTGGAGAAAAATACTAGAAGAAAATGTTTCTCTGATAAATACAAATACCCAATGAGAGCAAAACTTTACACTCTGGCAGAGCTTCAATTAGCTACAAACAGCTTTAGTGAAAAAAACTTTCTGGGCGAAGGATCTCTTGGTTCGGTTTACAAAGCTGTGTTTCCTGATGGCCAGACGTTGGCTGTGAAGCACATCAACATTGTAGGATTGTCTTTTAATGAAGAGGAACAATTCTTGGATGTCATATGGACTGCTTTGCATTTGAGGCACCCAAACATAGTACCACTTGTTGGTTATTGCATAGAGCATGGACAGCATCTTGTTGTCTACGAGTATGTTAGAAATTTATCCCTTGATGATGCTCTTCACAGTGATGCTTACAAGCCTCTGTCCTGGGGCCTCCGTCTCCAGATTGCTCTTGGTGTTGCTCAAGCTTTGGACCATCTGCACTCTACATTCTCACCTCCAGTTGCTCATAGAAACTTGAAGAGTGCCAATATCCTACTTGATGAAGAACTTGCTCCTAGAATCTGTGACTGCGGACTTGCGATTTTGAGGCCACTAACAAGCAATAGACTCAAGCTAAAGGTATATCTATACTTTCATAAACCAGTTCTATTTTCCACTAGACACCATCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

551

Amino Acids

60.88

Weight (kDa)

8.89

Isoelectric Point (pI)

46.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	32 - 70	5.3e-09	Leucine rich repeat N-terminal domain
LRR_8	PF13855	115 - 156	1.3e-06	Leucine rich repeat
LRR_4	PF12799	120 - 156	1.7e-06	Leucine Rich repeats (2 copies)
Pkinase	PF00069	373 - 521	2.6e-23	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	376 - 521	1.9e-21	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000664)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06820
fragaria_vesca	FvH4_6g22260 FvH4_6g22260 FvH4_6g22260 FvH4_6g22370 FvH4_6g22660 FvH4_6g22660
malus_domestica	MD12G1060500.v1.1 MD12G1060800.v1.1 MD13G1140200.v1.1 MD14G1060500.v1.1
prunus_persica	Prupe.6G256200_v2.0.a1 Prupe.6G256200_v2.0.a1 Prupe.7G074000_v2.0.a1 Prupe.7G074000_v2.0.a1
pyrus_communis	pycom14g04900
rosa_chinensis	RchiOBHm_Chr2g0158881 RchiOBHm_Chr3g0476831 RchiOBHm_Chr3g0476851 RchiOBHm_Chr3g0476861 RchiOBHm_Chr3g0480221
rosa_laevigata	RLG00000021120 RLG00000023509 RLG00000023748 RLG00000024125 RLG00000029770 RLG00000029771
rosa_multiflora	Rmu_co8335633.1_g000001 Rmu_sc0002267.1_g000010 Rmu_sc0002679.1_g000001 Rmu_sc0006148.1_g000002 Rmu_sc0008085.1_g000009 Rmu_sc0009296.1_g000001 Rmu_sc0009296.1_g000015 Rmu_sc0014404.1_g000008 Rmu_sc0041425.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090920 Rroxscaffold_4G00319720 Rroxscaffold_4G00319850 Rroxscaffold_6G00401690 Rroxscaffold_6G00404530 Rroxscaffold_6G00404980 Rroxscaffold_6G00409500
rosa_rugosa	Rorug02G0473000 Rorug03G0157100 Rorug03G0181200 Rorug03G0181300
rosa_samantha	Rh1AG112100 Rh1CG109100 Rh1DG113400 Rh1DG117300 Rh2AG539100 Rh2BG551800 Rh2BG551900 Rh2CG522500 Rh2DG561600 Rh3AG171500 Rh3AG207000 Rh3AG207400 Rh3AG233000 Rh3BG197100 Rh3BG239900 Rh3BG240000 Rh3BG265600 Rh3CG188000 Rh3CG188100 Rh3CG233700 Rh3CG262800 Rh3DG177300 Rh3DG177400 Rh3DG233700 Rh3DG258800
rosa_wichuraiana	Rw0G002320 Rw3G016860 Rw3G020460 Rw3G020930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	857
AccB1I	GGYRCC	2 cut(s)	68, 1278
AccB7I	CCANNNNNTGG	2 cut(s)	1189, 1301
AccI	GTMKAC	1 cut(s)	1338
AciI	CCGC	4 cut(s)	6, 89, 537, 1551
AclWI	GGATC	4 cut(s)	167, 180, 481, 1150
AcoI	YGGCCR	2 cut(s)	3, 1180
AcsI	RAATTY	6 cut(s)	361, 464, 571, 694, 886, 1354
AcuI	CTGAAG	1 cut(s)	167
AcyI	GRCGYC	1 cut(s)	69
AfaI	GTAC	2 cut(s)	660, 1293
AfiI	CCNNNNNNNGG	3 cut(s)	12, 1189, 1301
AgeI	ACCGGT	1 cut(s)	139
AgsI	TTSAA	4 cut(s)	289, 693, 1097, 1498
AjnI	CCWGG	3 cut(s)	642, 754, 1403
AjuI	GAANNNNNNNTTGG	2 cut(s)	1131, 1163
AleI	CACNNNNGTG	1 cut(s)	791
Alw21I	GWGCWC	2 cut(s)	276, 847
Alw26I	GTCTC	1 cut(s)	1421
Alw44I	GTGCAC	1 cut(s)	843
AlwI	GGATC	4 cut(s)	167, 180, 481, 1150
AoxI	GGCC	5 cut(s)	3, 123, 1180, 1408, 1568
ApaI	GGGCCC	1 cut(s)	127
ApaLI	GTGCAC	1 cut(s)	843
ApoI	RAATTY	6 cut(s)	361, 464, 571, 694, 886, 1354
AseI	ATTAAT	1 cut(s)	831
AsiGI	ACCGGT	1 cut(s)	139
AspLEI	GCGC	2 cut(s)	71, 676
AspS9I	GGNCC	5 cut(s)	123, 124, 329, 1408, 1450
AsuC2I	CCSGG	1 cut(s)	122
AsuHPI	GGTGA	3 cut(s)	388, 957, 1464
AvaII	GGWCC	2 cut(s)	329, 1450
AxyI	CCTNAGG	1 cut(s)	267
BaeGI	GKGCMC	2 cut(s)	127, 847
BalI	TGGCCA	1 cut(s)	1182
BamHI	GGATCC	1 cut(s)	172
BanI	GGYRCC	2 cut(s)	68, 1278
BanII	GRGCYC	3 cut(s)	127, 276, 755
Bbv12I	GWGCWC	2 cut(s)	276, 847
BccI	CCATC	6 cut(s)	158, 188, 911, 952, 1172, 1461
BceAI	ACGGC	2 cut(s)	20, 117
BciT130I	CCWGG	3 cut(s)	644, 756, 1405
BciVI	GTATCC	1 cut(s)	725
BcnI	CCSGG	1 cut(s)	122
BcoDI	GTCTC	1 cut(s)	1421
BfaI	CTAG	5 cut(s)	488, 791, 1026, 1536, 1638
BfmI	CTRYAG	2 cut(s)	356, 452
BfoI	RGCGCY	1 cut(s)	72
BfuI	GTATCC	1 cut(s)	725
BglII	AGATCT	1 cut(s)	403
BisI	GCNGC	2 cut(s)	6, 538
BlpI	GCTNAGC	1 cut(s)	492
BlsI	GCNGC	2 cut(s)	7, 539
Bme1390I	CCNGG	4 cut(s)	122, 644, 756, 1405
Bme18I	GGWCC	2 cut(s)	329, 1450
BmgT120I	GGNCC	5 cut(s)	123, 124, 329, 1408, 1450
BmiI	GGNNCC	8 cut(s)	70, 125, 174, 214, 330, 424, 1280, 1409
BmrFI	CCNGG	4 cut(s)	122, 644, 756, 1405
BmsI	GCATC	5 cut(s)	549, 806, 1336, 1360, 1375
BpmI	CTGGAG	5 cut(s)	777, 978, 1034, 1403, 1461
Bpu1102I	GCTNAGC	1 cut(s)	492
BpuEI	CTTGAG	3 cut(s)	865, 1425, 1574
BpuMI	CCSGG	1 cut(s)	122
BsaHI	GRCGYC	1 cut(s)	69
BsaJI	CCNNGG	7 cut(s)	36, 620, 642, 754, 861, 980, 1404
BsaWI	WCCGGW	2 cut(s)	139, 398
Bsc4I	CCNNNNNNNGG	3 cut(s)	12, 1189, 1301
Bse118I	RCCGGY	1 cut(s)	139
Bse1I	ACTGG	6 cut(s)	445, 475, 995, 1017, 1478, 1622
Bse21I	CCTNAGG	1 cut(s)	267
Bse3DI	GCAATG	1 cut(s)	346
BseBI	CCWGG	3 cut(s)	644, 756, 1405
BseDI	CCNNGG	7 cut(s)	36, 620, 642, 754, 861, 980, 1404
BseGI	GGATG	4 cut(s)	169, 199, 753, 1255
BseLI	CCNNNNNNNGG	3 cut(s)	12, 1189, 1301
BseMI	GCAATG	1 cut(s)	346
BseMII	CTCAG	3 cut(s)	258, 299, 483
BseNI	ACTGG	6 cut(s)	445, 475, 995, 1017, 1478, 1622
BseRI	GAGGAG	1 cut(s)	284
BseSI	GKGCMC	2 cut(s)	127, 847
BseYI	CCCAGC	1 cut(s)	679
BsgI	GTGCAG	1 cut(s)	1442
Bsh1285I	CGRYCG	1 cut(s)	161
BshFI	GGCC	5 cut(s)	5, 125, 1182, 1410, 1570
BshNI	GGYRCC	2 cut(s)	68, 1278
BshTI	ACCGGT	1 cut(s)	139
BsiEI	CGRYCG	1 cut(s)	161
BsiHKAI	GWGCWC	2 cut(s)	276, 847
BsiSI	CCGG	4 cut(s)	72, 121, 140, 399
BslFI	GGGAC	1 cut(s)	342
BslI	CCNNNNNNNGG	3 cut(s)	12, 1189, 1301
BsmAI	GTCTC	1 cut(s)	1421
BsmBI	CGTCTC	1 cut(s)	1421
BsmFI	GGGAC	1 cut(s)	342
BsnI	GGCC	5 cut(s)	5, 125, 1182, 1410, 1570
Bsp120I	GGGCCC	1 cut(s)	123
Bsp1286I	GDGCHC	4 cut(s)	127, 276, 755, 847
Bsp143I	GATC	6 cut(s)	158, 172, 403, 473, 508, 1142
Bsp1720I	GCTNAGC	1 cut(s)	492
BspACI	CCGC	4 cut(s)	6, 89, 537, 1551
BspANI	GGCC	5 cut(s)	5, 125, 1182, 1410, 1570
BspCNI	CTCAG	3 cut(s)	259, 300, 484
BspHI	TCATGA	1 cut(s)	109
BspLI	GGNNCC	8 cut(s)	70, 125, 174, 214, 330, 424, 1280, 1409
BspMAI	CTGCAG	1 cut(s)	456
BspPI	GGATC	4 cut(s)	167, 180, 481, 1150
BspQI	GCTCTTC	2 cut(s)	111, 1380
BspT107I	GGYRCC	2 cut(s)	68, 1278
BsrDI	GCAATG	1 cut(s)	346
BsrFI	RCCGGY	1 cut(s)	139
BsrI	ACTGG	6 cut(s)	445, 475, 995, 1017, 1478, 1622
BssAI	RCCGGY	1 cut(s)	139
BssECI	CCNNGG	7 cut(s)	36, 620, 642, 754, 861, 980, 1404
BssMI	GATC	6 cut(s)	158, 172, 403, 473, 508, 1142
BssNI	GRCGYC	1 cut(s)	69
BssT1I	CCWWGG	3 cut(s)	620, 861, 980
Bst2UI	CCWGG	3 cut(s)	644, 756, 1405
Bst4CI	ACNGT	1 cut(s)	1382
Bst6I	CTCTTC	5 cut(s)	111, 177, 1227, 1380, 1493
BstACI	GRCGYC	1 cut(s)	69
BstC8I	GCNNGC	1 cut(s)	843
BstDEI	CTNAG	3 cut(s)	267, 308, 492
BstDSI	CCRYGG	1 cut(s)	36
BstEII	GGTNACC	1 cut(s)	963
BstF5I	GGATG	4 cut(s)	169, 199, 753, 1255
BstH2I	RGCGCY	1 cut(s)	72
BstHHI	GCGC	2 cut(s)	71, 676
BstKTI	GATC	6 cut(s)	161, 175, 406, 476, 511, 1145
BstMAI	GTCTC	1 cut(s)	1421
BstMBI	GATC	6 cut(s)	158, 172, 403, 473, 508, 1142
BstMCI	CGRYCG	1 cut(s)	161
BstMWI	GCNNNNNNNGC	4 cut(s)	488, 680, 1276, 1324
BstNI	CCWGG	3 cut(s)	644, 756, 1405
BstPI	GGTNACC	1 cut(s)	963
BstSCI	CCNGG	4 cut(s)	120, 642, 754, 1403
BstSFI	CTRYAG	2 cut(s)	356, 452
BstSLI	GKGCMC	2 cut(s)	127, 847
BstX2I	RGATCY	3 cut(s)	172, 403, 1142
BstXI	CCANNNNNNTGG	1 cut(s)	576
BstYI	RGATCY	3 cut(s)	172, 403, 1142
Bsu36I	CCTNAGG	1 cut(s)	267
BsuI	GTATCC	1 cut(s)	725
BsuRI	GGCC	5 cut(s)	5, 125, 1182, 1410, 1570
BtgI	CCRYGG	1 cut(s)	36
BtsCI	GGATG	4 cut(s)	169, 199, 753, 1255
BtsI	GCAGTG	1 cut(s)	906
BtsIMutI	CAGTG	4 cut(s)	438, 468, 906, 1387
Cac8I	GCNNGC	1 cut(s)	843
CciI	TCATGA	1 cut(s)	109
CfoI	GCGC	2 cut(s)	71, 676
Cfr10I	RCCGGY	1 cut(s)	139
Cfr13I	GGNCC	5 cut(s)	123, 124, 329, 1408, 1450
Csp6I	GTAC	2 cut(s)	659, 1292
CspAI	ACCGGT	1 cut(s)	139
CviAII	CATG	8 cut(s)	110, 189, 204, 427, 599, 801, 970, 1319
CviQI	GTAC	2 cut(s)	659, 1292
DdeI	CTNAG	3 cut(s)	267, 308, 492
DinI	GGCGCC	1 cut(s)	70
DpnI	GATC	6 cut(s)	160, 174, 405, 475, 510, 1144
DpnII	GATC	6 cut(s)	158, 172, 403, 473, 508, 1142
DraI	TTTAAA	1 cut(s)	294
EaeI	YGGCCR	2 cut(s)	3, 1180
Eam1104I	CTCTTC	5 cut(s)	111, 177, 1227, 1380, 1493
EarI	CTCTTC	5 cut(s)	111, 177, 1227, 1380, 1493
Ecl136II	GAGCTC	1 cut(s)	274
Eco130I	CCWWGG	3 cut(s)	620, 861, 980
Eco24I	GRGCYC	3 cut(s)	127, 276, 755
Eco47I	GGWCC	2 cut(s)	329, 1450
Eco53kI	GAGCTC	1 cut(s)	274
Eco57I	CTGAAG	1 cut(s)	167
Eco81I	CCTNAGG	1 cut(s)	267
Eco91I	GGTNACC	1 cut(s)	963
EcoICRI	GAGCTC	1 cut(s)	274
EcoO109I	RGGNCCY	3 cut(s)	124, 329, 1408
EcoO65I	GGTNACC	1 cut(s)	963
EcoRI	GAATTC	1 cut(s)	886
EcoRII	CCWGG	3 cut(s)	642, 754, 1403
EcoT14I	CCWWGG	3 cut(s)	620, 861, 980
EcoT38I	GRGCYC	3 cut(s)	127, 276, 755
EgeI	GGCGCC	1 cut(s)	70
EheI	GGCGCC	1 cut(s)	70
ErhI	CCWWGG	3 cut(s)	620, 861, 980
Esp3I	CGTCTC	1 cut(s)	1421
FaeI	CATG	8 cut(s)	113, 192, 207, 430, 602, 804, 973, 1322
FalI	AAGNNNNNCTT	2 cut(s)	1131, 1163
FaqI	GGGAC	1 cut(s)	342
FatI	CATG	8 cut(s)	109, 188, 203, 426, 598, 800, 969, 1318
FauI	CCCGC	1 cut(s)	96
FauNDI	CATATG	1 cut(s)	1256
FblI	GTMKAC	1 cut(s)	1338
Fnu4HI	GCNGC	2 cut(s)	6, 538
FokI	GGATG	4 cut(s)	176, 206, 760, 1262
FriOI	GRGCYC	3 cut(s)	127, 276, 755
Fsp4HI	GCNGC	2 cut(s)	6, 538
FspBI	CTAG	5 cut(s)	488, 791, 1026, 1536, 1638
GlaI	GCGC	2 cut(s)	70, 675
GluI	GCNGC	2 cut(s)	6, 538
GsaI	CCCAGC	1 cut(s)	683
GsuI	CTGGAG	5 cut(s)	777, 978, 1034, 1403, 1461
HaeII	RGCGCY	1 cut(s)	72
HaeIII	GGCC	5 cut(s)	5, 125, 1182, 1410, 1570
HapII	CCGG	4 cut(s)	72, 121, 140, 399
HhaI	GCGC	2 cut(s)	71, 676
Hin1I	GRCGYC	1 cut(s)	69
Hin1II	CATG	8 cut(s)	113, 192, 207, 430, 602, 804, 973, 1322
Hin6I	GCGC	2 cut(s)	69, 674
HinP1I	GCGC	2 cut(s)	69, 674
HindIII	AAGCTT	2 cut(s)	851, 1443
HinfI	GANTC	7 cut(s)	185, 304, 433, 709, 783, 1539, 1587
HpaII	CCGG	4 cut(s)	72, 121, 140, 399
HphI	GGTGA	3 cut(s)	388, 957, 1464
Hpy166II	GTNNAC	4 cut(s)	845, 1000, 1159, 1339
Hpy188I	TCNGA	5 cut(s)	163, 246, 309, 513, 1045
Hpy188III	TCNNGA	7 cut(s)	110, 255, 266, 327, 635, 1175, 1420
Hpy8I	GTNNAC	4 cut(s)	845, 1000, 1159, 1339
Hpy99I	CGWCG	1 cut(s)	55
HpyAV	CCTTC	2 cut(s)	142, 1133
HpyCH4III	ACNGT	1 cut(s)	1382
HpyCH4IV	ACGT	1 cut(s)	1187
HpyCH4V	TGCA	6 cut(s)	454, 845, 932, 1270, 1311, 1459
HpyF10VI	GCNNNNNNNGC	4 cut(s)	488, 680, 1276, 1324
HpyF3I	CTNAG	3 cut(s)	267, 308, 492
HpySE526I	ACGT	1 cut(s)	1187
Hsp92I	GRCGYC	1 cut(s)	69
Hsp92II	CATG	8 cut(s)	113, 192, 207, 430, 602, 804, 973, 1322
HspAI	GCGC	2 cut(s)	69, 674
KasI	GGCGCC	1 cut(s)	68
Kzo9I	GATC	6 cut(s)	158, 172, 403, 473, 508, 1142
LguI	GCTCTTC	2 cut(s)	111, 1380
LmnI	GCTCC	3 cut(s)	74, 271, 1537
LweI	GCATC	5 cut(s)	549, 806, 1336, 1360, 1375
MaeI	CTAG	5 cut(s)	488, 791, 1026, 1536, 1638
MaeII	ACGT	1 cut(s)	1187
MaeIII	GTNAC	4 cut(s)	784, 872, 963, 1544
MalI	GATC	6 cut(s)	160, 174, 405, 475, 510, 1144
MboI	GATC	6 cut(s)	158, 172, 403, 473, 508, 1142
MfeI	CAATTG	1 cut(s)	836
MflI	RGATCY	3 cut(s)	172, 403, 1142
MhlI	GDGCHC	4 cut(s)	127, 276, 755, 847
MlsI	TGGCCA	1 cut(s)	1182
MluNI	TGGCCA	1 cut(s)	1182
Mly113I	GGCGCC	1 cut(s)	69
MlyI	GAGTC	3 cut(s)	194, 792, 1581
Mox20I	TGGCCA	1 cut(s)	1182
MscI	TGGCCA	1 cut(s)	1182
MseI	TTAA	5 cut(s)	129, 293, 365, 831, 1227
MslI	CAYNNNNRTG	2 cut(s)	791, 1383
Msp20I	TGGCCA	1 cut(s)	1182
MspA1I	CMGCKG	1 cut(s)	537
MspI	CCGG	4 cut(s)	72, 121, 140, 399
MspR9I	CCNGG	4 cut(s)	122, 644, 756, 1405
MunI	CAATTG	1 cut(s)	836
MvaI	CCWGG	3 cut(s)	644, 756, 1405
MwoI	GCNNNNNNNGC	4 cut(s)	488, 680, 1276, 1324
NarI	GGCGCC	1 cut(s)	69
NciI	CCSGG	1 cut(s)	122
NdeI	CATATG	1 cut(s)	1256
NdeII	GATC	6 cut(s)	158, 172, 403, 473, 508, 1142
NlaIII	CATG	8 cut(s)	113, 192, 207, 430, 602, 804, 973, 1322
NlaIV	GGNNCC	8 cut(s)	70, 125, 174, 214, 330, 424, 1280, 1409
NmuCI	GTSAC	3 cut(s)	784, 963, 1544
OliI	CACNNNNGTG	1 cut(s)	791
PagI	TCATGA	1 cut(s)	109
PciSI	GCTCTTC	2 cut(s)	111, 1380
PfeI	GAWTC	4 cut(s)	304, 433, 709, 1539
PflMI	CCANNNNNTGG	2 cut(s)	1189, 1301
PinAI	ACCGGT	1 cut(s)	139
PkrI	GCNGC	2 cut(s)	7, 539
Ple19I	CGATCG	1 cut(s)	161
PleI	GAGTC	3 cut(s)	193, 791, 1581
PluTI	GGCGCC	1 cut(s)	72
PpsI	GAGTC	3 cut(s)	193, 791, 1581
PpuMI	RGGWCCY	1 cut(s)	329
PshBI	ATTAAT	1 cut(s)	831
PsiI	TTATAA	1 cut(s)	857
Psp124BI	GAGCTC	1 cut(s)	276
Psp5II	RGGWCCY	1 cut(s)	329
Psp6I	CCWGG	3 cut(s)	642, 754, 1403
PspEI	GGTNACC	1 cut(s)	963
PspFI	CCCAGC	1 cut(s)	679
PspGI	CCWGG	3 cut(s)	642, 754, 1403
PspN4I	GGNNCC	8 cut(s)	70, 125, 174, 214, 330, 424, 1280, 1409
PspOMI	GGGCCC	1 cut(s)	123
PspPI	GGNCC	5 cut(s)	123, 124, 329, 1408, 1450
PspPPI	RGGWCCY	1 cut(s)	329
PstI	CTGCAG	1 cut(s)	456
PsuI	RGATCY	3 cut(s)	172, 403, 1142
PvuI	CGATCG	1 cut(s)	161
RsaI	GTAC	2 cut(s)	660, 1293
RsaNI	GTAC	2 cut(s)	659, 1292
RseI	CAYNNNNRTG	2 cut(s)	791, 1383
SacI	GAGCTC	1 cut(s)	276
SapI	GCTCTTC	2 cut(s)	111, 1380
SaqAI	TTAA	5 cut(s)	129, 293, 365, 831, 1227
SatI	GCNGC	2 cut(s)	6, 538
Sau3AI	GATC	6 cut(s)	158, 172, 403, 473, 508, 1142
Sau96I	GGNCC	5 cut(s)	123, 124, 329, 1408, 1450
SchI	GAGTC	3 cut(s)	194, 792, 1581
ScrFI	CCNGG	4 cut(s)	122, 644, 756, 1405
SduI	GDGCHC	4 cut(s)	127, 276, 755, 847
SfaNI	GCATC	5 cut(s)	549, 806, 1336, 1360, 1375
SfcI	CTRYAG	2 cut(s)	356, 452
SfoI	GGCGCC	1 cut(s)	70
SgrAI	CRCCGGYG	1 cut(s)	139
SinI	GGWCC	2 cut(s)	329, 1450
SmiMI	CAYNNNNRTG	2 cut(s)	791, 1383
SmlI	CTYRAG	3 cut(s)	880, 1440, 1589
SmoI	CTYRAG	3 cut(s)	880, 1440, 1589
SsiI	CCGC	4 cut(s)	6, 89, 537, 1551
SspDI	GGCGCC	1 cut(s)	68
SspMI	CTAG	5 cut(s)	488, 791, 1026, 1536, 1638
SstI	GAGCTC	1 cut(s)	276
StyD4I	CCNGG	4 cut(s)	120, 642, 754, 1403
StyI	CCWWGG	3 cut(s)	620, 861, 980
TaaI	ACNGT	1 cut(s)	1382
TaiI	ACGT	1 cut(s)	1190
TauI	GCSGC	2 cut(s)	8, 540
TfiI	GAWTC	4 cut(s)	304, 433, 709, 1539
Tru1I	TTAA	5 cut(s)	129, 293, 365, 831, 1227
Tru9I	TTAA	5 cut(s)	129, 293, 365, 831, 1227
TscAI	CASTG	4 cut(s)	445, 475, 913, 1387
TseFI	GTSAC	3 cut(s)	784, 963, 1544
Tsp45I	GTSAC	3 cut(s)	784, 963, 1544
TspDTI	ATGAA	8 cut(s)	34, 98, 192, 587, 677, 1245, 1536, 1604
TspGWI	ACGGA	2 cut(s)	28, 1403
TspRI	CASTG	4 cut(s)	445, 475, 913, 1387
Van91I	CCANNNNNTGG	2 cut(s)	1189, 1301
VneI	GTGCAC	1 cut(s)	843
VpaK11BI	GGWCC	2 cut(s)	329, 1450
VspI	ATTAAT	1 cut(s)	831
XapI	RAATTY	6 cut(s)	361, 464, 571, 694, 886, 1354
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1427
XmiI	GTMKAC	1 cut(s)	1338
XspI	CTAG	5 cut(s)	488, 791, 1026, 1536, 1638

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.