RchiOBHm_Chr3g0480221

Protein STRUBBELIG-RECEPTOR FAMILY 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000664
Physical Location & Seq
Forward (+)
25980514 .. 25986182
5669 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44525

Sequence Viewer

Length: 2223 bp
ATGGCGAAGTTGTACCGGTTCGTGTACTTCATGGCGGTTTTCTTCGCGGCGGTTCTGGTGGCGTCCCCGGCTGGGGCTTTTACTGATCCACTTGACGTCGTAGCTCTTAATGATCTTTACAAGACCCTCAACCAACCGCCACAGCTGAAGGGTTGGAGATTGGATGGTGGGGATCCTTGCGATGAATCGTGGAATGGAGTGTCATGCTTTGGCTCTTCTGTGATATACCTTAAACTTAATGGTCTAAACCTCTCTGGGTATATTACTGCCGAGATCTATAATCTCTACAGTTTGAAGCAACTGGATATTAGTTCCAATACCATTGGTGGTGAAATTCCATATGTCTTACCCCCCAATGCGACTCATATAAATATGGCATACAACTATTTCATCCAAAATATTCCCCTATCCTTACCTGGCATGAAAAGTCTCCGACATTTGAATCTAAGCCACAATTTGTTATCTGGACCCATTGGCAATGTTTTTACCGGCCTACAGAATTTAAGAGAAATGGATTTGTCATATAATAACTTTACTGGAGATCTGCCAAGTTCATTTGGATCCCTCACAAATCTCACTGGACTGTACTTGCAGAAGAACCAATTCACTGGATCAGTTGCTTACCTAGCCGAACTTCCACTAATTGATCTGAACATCCAAGATAATTATTTCAGTGGTATCATCCCCAGTCATTTTCAGTCCATACCAAATTTATGGATTGGAGGAAATAGTTTTCATCTTCCAGGGGACAACTATCCACCCTGGGATTTCCCTCTGGAAACGCAGACAAGTGTCATTGAACAGAACATCCACCCCCCTCCAGCAACTCAGTCAAGTGCCATGGAGAAGAGCAAGCCCTCTACCAAATTAGGTGGACACAAGAAGAGGAGGCTTGGCCCTGGAGGAATAGCTTTTATGATTGGTGGAGGAACTCTAGTCGCATCATGTGTGGCACTTTACATTGCAGTTCGTATCAAACAATCACGTGCACAGAAGCTTATAACTTTGGGGGGCAGTAATAGTTCAATCCACTCTTTCTCAGTCATAAATACGGCCAAAGATAGTCCTATGAATGCACGAGAAGAAAGCCCACCAATACTGACTTTCAGGCCACCCATTCTACTTCCAAGGCGACTACCTCCAGTTTATCATAGCAGAGTAGAGAAAACTAGAAGAAAAAGTTTCTCTGATAAATGCAGTTTCCCAGTGAGAATCAAACTATATACTGTGGCAGAGCTTCAGTCAGCTACGAACAGCTTTGCAGAAGAAAAGTTTCTGGGCCAAGGATCTCTTGGTTCTGTTTACAAAGCCGAGTTTCCTGATGGCCAAATTTTGGCTGTAAAGAACATCAAGATGTCTGGACTCTCTTTCCATGAAGAAGAACAATTCCTGGATGTCGTTTGGACTGTTTCGCGTTTGATGCACCCAAACATTGTACCACTTGTTGGCTATTGTGTGGAGAATGGACAACATCTCCTTGTGCATGAATATGTTGGAAATTTATCCCTTGATGAGGCACTTCACAGCGATGCATACAAGCCTCTGTCCTGGGGCCTCCGTCTCCAAATTGCTCTTGGTGTTGCTCAAGCTTTGGACTATCTGCACTCCACATTGTCGCCTTCAGTAGCTCATGGCAACTTGAAGAGCGAAAATATCTTACTTGATGAAGAACTTGCTCCTCATATATGTGACTGCGGACTTGCTATTTTGAGGCCACTTACAAGCAATAGTCTTAAACTGAAGGCTTCAGAAAATGCTGTTGGTGACACTGGCTACATTGCACCTGAGCATGGCCTACCAGGGTTTGATAAGACAAAGAGTGACATCTATGCCTTCGGAGTGCTGCTTTTGGAGCTCTTAACAGGAAGGAGACCTTCTGATAGTTCAAGACCAAGGGAAGAGCAATCACTGGTGAAATGGGCTTCATTGCGGCTTCATGACACTGAGAGTCTGGATGAGATGATCGATCCAGGCATTAAAAAGACATGTTCCTCCAAGGCTCTCTCAAATTTTGCTGATATTATCTCACTCTGTATACAGCCAGTGAAGGAATTCAGACCGCCAATGTCTGAAGTTGTGGGATCTCTAACACAACTAATGCAGAAGCTCAAGGGGAAAGGCAATGGAGCGGATGGTACACAAGTTGACCCCTTCGAGAGATCTTTTCGTTCAACCAACACCCGCTTCATGGGCTCACCTACATTGAGCTATTTTTCCATTTGA

Protein Analysis

740

Amino Acids

81.38

Weight (kDa)

7.03

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 69 5.6e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 161 - 202 2.8e-07 Leucine rich repeat
Pkinase PF00069 421 - 693 6.6e-33 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 423 - 695 1.6e-33 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000664)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06820
fragaria_vesca FvH4_6g22260 FvH4_6g22260 FvH4_6g22260 FvH4_6g22370 FvH4_6g22660 FvH4_6g22660
malus_domestica MD12G1060500.v1.1 MD12G1060800.v1.1 MD13G1140200.v1.1 MD14G1060500.v1.1
prunus_persica Prupe.6G256200_v2.0.a1 Prupe.6G256200_v2.0.a1 Prupe.7G074000_v2.0.a1 Prupe.7G074000_v2.0.a1
pyrus_communis pycom14g04900
rosa_chinensis RchiOBHm_Chr2g0158881 RchiOBHm_Chr3g0476831 RchiOBHm_Chr3g0476851 RchiOBHm_Chr3g0476861 RchiOBHm_Chr3g0480221
rosa_laevigata RLG00000021120 RLG00000023509 RLG00000023748 RLG00000024125 RLG00000029770 RLG00000029771
rosa_multiflora Rmu_co8335633.1_g000001 Rmu_sc0002267.1_g000010 Rmu_sc0002679.1_g000001 Rmu_sc0006148.1_g000002 Rmu_sc0008085.1_g000009 Rmu_sc0009296.1_g000001 Rmu_sc0009296.1_g000015 Rmu_sc0014404.1_g000008 Rmu_sc0041425.1_g000001
rosa_roxburghii Rroxscaffold_2G00090920 Rroxscaffold_4G00319720 Rroxscaffold_4G00319850 Rroxscaffold_6G00401690 Rroxscaffold_6G00404530 Rroxscaffold_6G00404980 Rroxscaffold_6G00409500
rosa_rugosa Rorug02G0473000 Rorug03G0157100 Rorug03G0181200 Rorug03G0181300
rosa_samantha Rh1AG112100 Rh1CG109100 Rh1DG113400 Rh1DG117300 Rh2AG539100 Rh2BG551800 Rh2BG551900 Rh2CG522500 Rh2DG561600 Rh3AG171500 Rh3AG207000 Rh3AG207400 Rh3AG233000 Rh3BG197100 Rh3BG239900 Rh3BG240000 Rh3BG265600 Rh3CG188000 Rh3CG188100 Rh3CG233700 Rh3CG262800 Rh3DG177300 Rh3DG177400 Rh3DG233700 Rh3DG258800
rosa_wichuraiana Rw0G002320 Rw3G016860 Rw3G020460 Rw3G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1001
AatII GACGTC 1 cut(s) 99
AccB7I CCANNNNNTGG 2 cut(s) 1333, 1445
AccBSI CCGCTC 1 cut(s) 2129
AccI GTMKAC 1 cut(s) 2035
AccII CGCG 2 cut(s) 47, 1414
AciI CCGC 9 cut(s) 35, 47, 50, 137, 1695, 1930, 2060, 2129, 2182
AclWI GGATC 9 cut(s) 80, 167, 180, 555, 568, 619, 1294, 1961, 2089
AcoI YGGCCR 2 cut(s) 1053, 1324
AcsI RAATTY 7 cut(s) 333, 499, 709, 1329, 1498, 2008, 2051
AcuI CTGAAG 6 cut(s) 167, 1223, 1605, 1731, 1760, 2091
AcvI CACGTG 1 cut(s) 986
AcyI GRCGYC 2 cut(s) 62, 96
AfaI GTAC 5 cut(s) 14, 26, 587, 1437, 2137
AfiI CCNNNNNNNGG 7 cut(s) 72, 73, 1333, 1445, 1513, 1790, 2188
AflIII ACRYGT 1 cut(s) 1985
AgeI ACCGGT 1 cut(s) 15
AgsI TTSAA 7 cut(s) 295, 442, 800, 1026, 1642, 1887, 2172
AjnI CCWGG 8 cut(s) 415, 742, 761, 898, 1389, 1547, 1798, 1969
AjuI GAANNNNNNNTTGG 4 cut(s) 1743, 1775, 2168, 2200
AloI GAACNNNNNNTCC 2 cut(s) 792, 824
Alw21I GWGCWC 2 cut(s) 991, 1857
Alw26I GTCTC 3 cut(s) 434, 1565, 1864
Alw44I GTGCAC 1 cut(s) 987
AlwI GGATC 9 cut(s) 80, 167, 180, 555, 568, 619, 1294, 1961, 2089
AoxI GGCC 9 cut(s) 490, 895, 1053, 1109, 1279, 1324, 1552, 1712, 1792
ApaLI GTGCAC 1 cut(s) 987
ApeKI GCWGC 1 cut(s) 1843
ApoI RAATTY 7 cut(s) 333, 499, 709, 1329, 1498, 2008, 2051
AsiGI ACCGGT 1 cut(s) 15
Asp700I GAANNNNTTC 3 cut(s) 602, 1272, 2051
AspS9I GGNCC 4 cut(s) 467, 896, 1279, 1552
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 4 cut(s) 341, 1775, 1924, 2187
AvaII GGWCC 1 cut(s) 467
BaeGI GKGCMC 1 cut(s) 991
BaeI ACNNNNGTAYC 2 cut(s) 2127, 2160
BalI TGGCCA 1 cut(s) 1326
BamHI GGATCC 2 cut(s) 172, 560
BanII GRGCYC 2 cut(s) 1857, 2195
BauI CACGAG 1 cut(s) 1077
BbrPI CACGTG 1 cut(s) 986
Bbv12I GWGCWC 2 cut(s) 991, 1857
BbvI GCAGC 1 cut(s) 1830
BccI CCATC 3 cut(s) 158, 1316, 2126
BceAI ACGGC 1 cut(s) 1068
BciT130I CCWGG 8 cut(s) 417, 744, 763, 900, 1391, 1549, 1800, 1971
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 3 cut(s) 434, 1565, 1864
BfaI CTAG 3 cut(s) 626, 935, 1170
BfmI CTRYAG 2 cut(s) 286, 494
BglII AGATCT 3 cut(s) 273, 541, 2159
BisI GCNGC 3 cut(s) 48, 1844, 1931
BlsI GCNGC 3 cut(s) 49, 1845, 1932
Bme1390I CCNGG 9 cut(s) 68, 417, 744, 763, 900, 1391, 1549, 1800, 1971
Bme18I GGWCC 1 cut(s) 467
BmgT120I GGNCC 4 cut(s) 467, 896, 1279, 1552
BmiI GGNNCC 4 cut(s) 174, 469, 562, 1553
BmrFI CCNGG 9 cut(s) 68, 417, 744, 763, 900, 1391, 1549, 1800, 1971
BmrI ACTGGG 2 cut(s) 681, 1199
BmsI GCATC 3 cut(s) 950, 1410, 1519
BmuI ACTGGG 2 cut(s) 681, 1199
BoxI GACNNNNGTC 1 cut(s) 791
BpmI CTGGAG 4 cut(s) 558, 804, 921, 1125
Bpu10I CCTNAGC 1 cut(s) 1785
BpuEI CTTGAG 2 cut(s) 1569, 2093
BpuMI CCSGG 1 cut(s) 68
Bsa29I ATCGAT 1 cut(s) 1965
BsaAI YACGTR 1 cut(s) 986
BsaHI GRCGYC 2 cut(s) 62, 96
BsaI GGTCTC 1 cut(s) 1864
BsaWI WCCGGW 1 cut(s) 15
BsaXI ACNNNNNCTCC 2 cut(s) 1458, 1488
Bsc4I CCNNNNNNNGG 7 cut(s) 72, 73, 1333, 1445, 1513, 1790, 2188
Bse118I RCCGGY 2 cut(s) 15, 488
Bse3DI GCAATG 5 cut(s) 484, 960, 1776, 1925, 2128
BseBI CCWGG 8 cut(s) 417, 744, 763, 900, 1391, 1549, 1800, 1971
BseCI ATCGAT 1 cut(s) 1965
BseGI GGATG 8 cut(s) 169, 390, 654, 681, 807, 1399, 1960, 2137
BseLI CCNNNNNNNGG 7 cut(s) 72, 73, 1333, 1445, 1513, 1790, 2188
BseMI GCAATG 5 cut(s) 484, 960, 1776, 1925, 2128
BseMII CTCAG 4 cut(s) 842, 1053, 1776, 1935
BseRI GAGGAG 2 cut(s) 901, 1668
BseSI GKGCMC 1 cut(s) 991
BseXI GCAGC 1 cut(s) 1830
BseYI CCCAGC 1 cut(s) 71
BsgI GTGCAG 1 cut(s) 1586
Bsh1236I CGCG 2 cut(s) 47, 1414
BshFI GGCC 9 cut(s) 492, 897, 1055, 1111, 1281, 1326, 1554, 1714, 1794
BshTI ACCGGT 1 cut(s) 15
BshVI ATCGAT 1 cut(s) 1965
BsiHKAI GWGCWC 2 cut(s) 991, 1857
BsiSI CCGG 3 cut(s) 16, 68, 489
BslFI GGGAC 2 cut(s) 49, 761
BslI CCNNNNNNNGG 7 cut(s) 72, 73, 1333, 1445, 1513, 1790, 2188
BsmAI GTCTC 3 cut(s) 434, 1565, 1864
BsmBI CGTCTC 1 cut(s) 1565
BsmFI GGGAC 2 cut(s) 49, 761
BsmI GAATGC 1 cut(s) 1078
BsnI GGCC 9 cut(s) 492, 897, 1055, 1111, 1281, 1326, 1554, 1714, 1794
Bso31I GGTCTC 1 cut(s) 1864
Bsp1286I GDGCHC 3 cut(s) 991, 1857, 2195
Bsp19I CCATGG 1 cut(s) 840
BspACI CCGC 9 cut(s) 35, 47, 50, 137, 1695, 1930, 2060, 2129, 2182
BspANI GGCC 9 cut(s) 492, 897, 1055, 1111, 1281, 1326, 1554, 1714, 1794
BspCNI CTCAG 4 cut(s) 841, 1052, 1777, 1936
BspDI ATCGAT 1 cut(s) 1965
BspFNI CGCG 2 cut(s) 47, 1414
BspHI TCATGA 1 cut(s) 1936
BspLI GGNNCC 4 cut(s) 174, 469, 562, 1553
BspPI GGATC 9 cut(s) 80, 167, 180, 555, 568, 619, 1294, 1961, 2089
BspQI GCTCTTC 4 cut(s) 220, 842, 1637, 1893
BspTNI GGTCTC 1 cut(s) 1864
BsrBI CCGCTC 1 cut(s) 2129
BsrDI GCAATG 5 cut(s) 484, 960, 1776, 1925, 2128
BsrFI RCCGGY 2 cut(s) 15, 488
BssAI RCCGGY 2 cut(s) 15, 488
BssNAI GTATAC 1 cut(s) 2036
BssNI GRCGYC 2 cut(s) 62, 96
BssSI CACGAG 1 cut(s) 1077
BssT1I CCWWGG 5 cut(s) 840, 1127, 1282, 1892, 1995
Bst1107I GTATAC 1 cut(s) 2036
Bst2BI CACGAG 1 cut(s) 1077
Bst2UI CCWGG 8 cut(s) 417, 744, 763, 900, 1391, 1549, 1800, 1971
Bst4CI ACNGT 4 cut(s) 290, 585, 1228, 1408
Bst6I CTCTTC 5 cut(s) 220, 842, 878, 1637, 1893
BstACI GRCGYC 2 cut(s) 62, 96
BstBAI YACGTR 1 cut(s) 986
BstC8I GCNNGC 1 cut(s) 854
BstDEI CTNAG 5 cut(s) 446, 828, 1039, 1785, 1944
BstDSI CCRYGG 1 cut(s) 840
BstENI CCTNNNNNAGG 1 cut(s) 1511
BstF5I GGATG 8 cut(s) 169, 390, 654, 681, 807, 1399, 1960, 2137
BstFNI CGCG 2 cut(s) 47, 1414
BstMAI GTCTC 3 cut(s) 434, 1565, 1864
BstMWI GCNNNNNNNGC 5 cut(s) 68, 626, 1420, 1852, 2190
BstNI CCWGG 8 cut(s) 417, 744, 763, 900, 1391, 1549, 1800, 1971
BstNSI RCATGY 1 cut(s) 1989
BstPAI GACNNNNGTC 1 cut(s) 791
BstSCI CCNGG 9 cut(s) 66, 415, 742, 761, 898, 1389, 1547, 1798, 1969
BstSFI CTRYAG 2 cut(s) 286, 494
BstSLI GKGCMC 1 cut(s) 991
BstUI CGCG 2 cut(s) 47, 1414
BstV1I GCAGC 1 cut(s) 1830
BstX2I RGATCY 7 cut(s) 172, 273, 541, 560, 1286, 2081, 2159
BstXI CCANNNNNNTGG 2 cut(s) 608, 714
BstYI RGATCY 7 cut(s) 172, 273, 541, 560, 1286, 2081, 2159
BstZ17I GTATAC 1 cut(s) 2036
Bsu15I ATCGAT 1 cut(s) 1965
BsuRI GGCC 9 cut(s) 492, 897, 1055, 1111, 1281, 1326, 1554, 1714, 1794
BsuTUI ATCGAT 1 cut(s) 1965
BtgI CCRYGG 1 cut(s) 840
BtgZI GCGATG 2 cut(s) 195, 1542
BtsCI GGATG 8 cut(s) 169, 390, 654, 681, 807, 1399, 1960, 2137
BtsIMutI CAGTG 8 cut(s) 576, 606, 679, 1212, 1767, 1907, 1941, 2049
Cac8I GCNNGC 1 cut(s) 854
CciI TCATGA 1 cut(s) 1936
Cfr10I RCCGGY 2 cut(s) 15, 488
Cfr13I GGNCC 4 cut(s) 467, 896, 1279, 1552
ClaI ATCGAT 1 cut(s) 1965
CseI GACGC 1 cut(s) 51
Csp6I GTAC 5 cut(s) 13, 25, 586, 1436, 2136
CspAI ACCGGT 1 cut(s) 15
CspCI CAANNNNNGTGG 2 cut(s) 853, 888
CviQI GTAC 5 cut(s) 13, 25, 586, 1436, 2136
DdeI CTNAG 5 cut(s) 446, 828, 1039, 1785, 1944
EaeI YGGCCR 2 cut(s) 1053, 1324
Eam1104I CTCTTC 5 cut(s) 220, 842, 878, 1637, 1893
EarI CTCTTC 5 cut(s) 220, 842, 878, 1637, 1893
Ecl136II GAGCTC 1 cut(s) 1855
Eco130I CCWWGG 5 cut(s) 840, 1127, 1282, 1892, 1995
Eco24I GRGCYC 2 cut(s) 1857, 2195
Eco31I GGTCTC 1 cut(s) 1864
Eco47I GGWCC 1 cut(s) 467
Eco53kI GAGCTC 1 cut(s) 1855
Eco57I CTGAAG 6 cut(s) 167, 1223, 1605, 1731, 1760, 2091
Eco72I CACGTG 1 cut(s) 986
EcoICRI GAGCTC 1 cut(s) 1855
EcoNI CCTNNNNNAGG 1 cut(s) 1511
EcoO109I RGGNCCY 1 cut(s) 1552
EcoRI GAATTC 1 cut(s) 2051
EcoRII CCWGG 8 cut(s) 415, 742, 761, 898, 1389, 1547, 1798, 1969
EcoT14I CCWWGG 5 cut(s) 840, 1127, 1282, 1892, 1995
EcoT22I ATGCAT 1 cut(s) 1534
EcoT38I GRGCYC 2 cut(s) 1857, 2195
ErhI CCWWGG 5 cut(s) 840, 1127, 1282, 1892, 1995
Esp3I CGTCTC 1 cut(s) 1565
FalI AAGNNNNNCTT 4 cut(s) 1275, 1307, 1858, 1890
FaqI GGGAC 2 cut(s) 49, 761
FauI CCCGC 1 cut(s) 2189
FauNDI CATATG 1 cut(s) 340
FblI GTMKAC 1 cut(s) 2035
Fnu4HI GCNGC 3 cut(s) 48, 1844, 1931
FokI GGATG 8 cut(s) 176, 377, 641, 668, 794, 1406, 1967, 2144
FriOI GRGCYC 2 cut(s) 1857, 2195
Fsp4HI GCNGC 3 cut(s) 48, 1844, 1931
FspBI CTAG 3 cut(s) 626, 935, 1170
GluI GCNGC 3 cut(s) 48, 1844, 1931
GsaI CCCAGC 1 cut(s) 75
GsuI CTGGAG 4 cut(s) 558, 804, 921, 1125
HaeIII GGCC 9 cut(s) 492, 897, 1055, 1111, 1281, 1326, 1554, 1714, 1794
HapII CCGG 3 cut(s) 16, 68, 489
HgaI GACGC 1 cut(s) 51
Hin1I GRCGYC 2 cut(s) 62, 96
HincII GTYRAC 1 cut(s) 2146
HindII GTYRAC 1 cut(s) 2146
HindIII AAGCTT 2 cut(s) 995, 1587
HinfI GANTC 6 cut(s) 185, 361, 442, 1212, 1362, 1948
HpaII CCGG 3 cut(s) 16, 68, 489
HphI GGTGA 4 cut(s) 341, 1775, 1924, 2187
Hpy166II GTNNAC 7 cut(s) 25, 875, 989, 1303, 2036, 2138, 2146
Hpy188I TCNGA 8 cut(s) 434, 651, 1189, 1750, 1838, 1879, 2057, 2071
Hpy188III TCNNGA 9 cut(s) 465, 776, 1319, 1351, 1359, 1887, 1937, 1952, 2155
Hpy8I GTNNAC 7 cut(s) 25, 875, 989, 1303, 2036, 2138, 2146
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 8 cut(s) 142, 1629, 1735, 1843, 1860, 1884, 2041, 2161
HpyCH4III ACNGT 4 cut(s) 290, 585, 1228, 1408
HpyCH4IV ACGT 2 cut(s) 96, 985
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 626, 1420, 1852, 2190
HpyF3I CTNAG 5 cut(s) 446, 828, 1039, 1785, 1944
HpySE526I ACGT 2 cut(s) 96, 985
Hsp92I GRCGYC 2 cut(s) 62, 96
LguI GCTCTTC 4 cut(s) 220, 842, 1637, 1893
LmnI GCTCC 3 cut(s) 1681, 1852, 2126
Lsp1109I GCAGC 1 cut(s) 1830
LweI GCATC 3 cut(s) 950, 1410, 1519
MaeI CTAG 3 cut(s) 626, 935, 1170
MaeII ACGT 2 cut(s) 96, 985
MaeIII GTNAC 3 cut(s) 1688, 1763, 1820
MbiI CCGCTC 1 cut(s) 2129
MflI RGATCY 7 cut(s) 172, 273, 541, 560, 1286, 2081, 2159
MhlI GDGCHC 3 cut(s) 991, 1857, 2195
MlsI TGGCCA 1 cut(s) 1326
MluNI TGGCCA 1 cut(s) 1326
MlyI GAGTC 3 cut(s) 355, 1356, 1957
MmeI TCCRAC 3 cut(s) 134, 457, 1474
Mox20I TGGCCA 1 cut(s) 1326
Mph1103I ATGCAT 1 cut(s) 1534
MroXI GAANNNNTTC 3 cut(s) 602, 1272, 2051
MscI TGGCCA 1 cut(s) 1326
MseI TTAA 7 cut(s) 108, 231, 237, 503, 1734, 1859, 1977
MslI CAYNNNNRTG 4 cut(s) 1352, 1488, 1527, 1686
Msp20I TGGCCA 1 cut(s) 1326
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 3 cut(s) 16, 68, 489
MspR9I CCNGG 9 cut(s) 68, 417, 744, 763, 900, 1391, 1549, 1800, 1971
Mva1269I GAATGC 1 cut(s) 1078
MvaI CCWGG 8 cut(s) 417, 744, 763, 900, 1391, 1549, 1800, 1971
MvnI CGCG 2 cut(s) 47, 1414
MwoI GCNNNNNNNGC 5 cut(s) 68, 626, 1420, 1852, 2190
NciI CCSGG 1 cut(s) 68
NcoI CCATGG 1 cut(s) 840
NdeI CATATG 1 cut(s) 340
NlaIV GGNNCC 4 cut(s) 174, 469, 562, 1553
NmeAIII GCCGAG 2 cut(s) 295, 1336
NmuCI GTSAC 3 cut(s) 1688, 1763, 1820
NsiI ATGCAT 1 cut(s) 1534
NspI RCATGY 1 cut(s) 1989
PagI TCATGA 1 cut(s) 1936
PasI CCCWGGG 1 cut(s) 762
PciI ACATGT 1 cut(s) 1985
PciSI GCTCTTC 4 cut(s) 220, 842, 1637, 1893
PctI GAATGC 1 cut(s) 1078
PdmI GAANNNNTTC 3 cut(s) 602, 1272, 2051
PfeI GAWTC 3 cut(s) 185, 442, 1212
PflMI CCANNNNNTGG 2 cut(s) 1333, 1445
PfoI TCCNGGA 1 cut(s) 1389
PinAI ACCGGT 1 cut(s) 15
PkrI GCNGC 3 cut(s) 49, 1845, 1932
PleI GAGTC 3 cut(s) 355, 1356, 1956
PmaCI CACGTG 1 cut(s) 986
PmlI CACGTG 1 cut(s) 986
PpsI GAGTC 3 cut(s) 355, 1356, 1956
Ppu21I YACGTR 1 cut(s) 986
PscI ACATGT 1 cut(s) 1985
PshAI GACNNNNGTC 1 cut(s) 791
PsiI TTATAA 1 cut(s) 1001
Psp124BI GAGCTC 1 cut(s) 1857
Psp6I CCWGG 8 cut(s) 415, 742, 761, 898, 1389, 1547, 1798, 1969
PspCI CACGTG 1 cut(s) 986
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 8 cut(s) 415, 742, 761, 898, 1389, 1547, 1798, 1969
PspN4I GGNNCC 4 cut(s) 174, 469, 562, 1553
PspPI GGNCC 4 cut(s) 467, 896, 1279, 1552
PsuI RGATCY 7 cut(s) 172, 273, 541, 560, 1286, 2081, 2159
PvuII CAGCTG 1 cut(s) 145
RsaI GTAC 5 cut(s) 14, 26, 587, 1437, 2137
RsaNI GTAC 5 cut(s) 13, 25, 586, 1436, 2136
RseI CAYNNNNRTG 4 cut(s) 1352, 1488, 1527, 1686
SacI GAGCTC 1 cut(s) 1857
SapI GCTCTTC 4 cut(s) 220, 842, 1637, 1893
SaqAI TTAA 7 cut(s) 108, 231, 237, 503, 1734, 1859, 1977
SatI GCNGC 3 cut(s) 48, 1844, 1931
Sau96I GGNCC 4 cut(s) 467, 896, 1279, 1552
SchI GAGTC 3 cut(s) 355, 1356, 1957
ScrFI CCNGG 9 cut(s) 68, 417, 744, 763, 900, 1391, 1549, 1800, 1971
SduI GDGCHC 3 cut(s) 991, 1857, 2195
SfaNI GCATC 3 cut(s) 950, 1410, 1519
SfcI CTRYAG 2 cut(s) 286, 494
SinI GGWCC 1 cut(s) 467
SmiMI CAYNNNNRTG 4 cut(s) 1352, 1488, 1527, 1686
SmlI CTYRAG 2 cut(s) 1584, 2108
SmoI CTYRAG 2 cut(s) 1584, 2108
SsiI CCGC 9 cut(s) 35, 47, 50, 137, 1695, 1930, 2060, 2129, 2182
SspI AATATT 1 cut(s) 400
SspMI CTAG 3 cut(s) 626, 935, 1170
SstI GAGCTC 1 cut(s) 1857
StyD4I CCNGG 9 cut(s) 66, 415, 742, 761, 898, 1389, 1547, 1798, 1969
StyI CCWWGG 5 cut(s) 840, 1127, 1282, 1892, 1995
TaaI ACNGT 4 cut(s) 290, 585, 1228, 1408
TaiI ACGT 2 cut(s) 99, 988
TaqI TCGA 2 cut(s) 1965, 2154
TatI WGTACW 2 cut(s) 24, 585
TauI GCSGC 2 cut(s) 50, 1933
TfiI GAWTC 3 cut(s) 185, 442, 1212
Tru1I TTAA 7 cut(s) 108, 231, 237, 503, 1734, 1859, 1977
Tru9I TTAA 7 cut(s) 108, 231, 237, 503, 1734, 1859, 1977
TscAI CASTG 8 cut(s) 583, 613, 679, 1212, 1774, 1914, 1948, 2049
TseFI GTSAC 3 cut(s) 1688, 1763, 1820
TseI GCWGC 1 cut(s) 1843
Tsp45I GTSAC 3 cut(s) 1688, 1763, 1820
TspGWI ACGGA 1 cut(s) 1547
TspRI CASTG 8 cut(s) 583, 613, 679, 1212, 1774, 1914, 1948, 2049
Van91I CCANNNNNTGG 2 cut(s) 1333, 1445
VneI GTGCAC 1 cut(s) 987
VpaK11BI GGWCC 1 cut(s) 467
XagI CCTNNNNNAGG 1 cut(s) 1511
XapI RAATTY 7 cut(s) 333, 499, 709, 1329, 1498, 2008, 2051
XceI RCATGY 1 cut(s) 1989
XcmI CCANNNNNNNNNTGG 2 cut(s) 1289, 1571
XmiI GTMKAC 1 cut(s) 2035
XmnI GAANNNNTTC 3 cut(s) 602, 1272, 2051
XspI CTAG 3 cut(s) 626, 935, 1170
ZraI GACGTC 1 cut(s) 97
Zsp2I ATGCAT 1 cut(s) 1534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.