Rw3G016860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000664
Physical Location & Seq
Reverse (-)
18088632 .. 18091224
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G016860.1

Sequence Viewer

Length: 570 bp
ATGGCGACCAAGTATCAGTACTTGTTTTTGACGGTGCTTTTCATGGCGGTTGTTATGGCTTCTCTGACTGAGGCTTTTACTGATCCACTCGACGGTCAACACCGCATTGGTGCTGCTGCTCAACCTGATCAGAACGACAGCATTCCCGCCGTTGCTCAACCAGGGCAGCAAGCTGCCATTGCTGCTGCTGCTCAACCAGGCCAACAACCTGCCGTTGCTACTGTTGCTCAGCCAGGCCAACAAGCCCCCATTGCTGGTTCTGCTCAATCCCCCTTCATTGTTTCCCCTGATCCTGATGTCTTACCTGCCACTTACATCTCCTCAAAGAAGAAACGGAAAATATCCTTTGGAACATTTGAAGGTGGTACGTGGCAGATTTCCGATGGAATTAGATTTCATACTGCAGATTCTCGTGAGAAAGTGATAAAACTGGCATCTCCTGCTCCAAGGGTTGAGCAGCCAAGGAGAAAGATGAGTTTCATAAACCAAGACAATGAAGTAGAAGAGTATGCAATGGTAGATGATGATGATGATGATGATGAAGAATGTAAGAAAAAGACACTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

20.49

Weight (kDa)

4.81

Isoelectric Point (pI)

55.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000664)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06820
fragaria_vesca FvH4_6g22260 FvH4_6g22260 FvH4_6g22260 FvH4_6g22370 FvH4_6g22660 FvH4_6g22660
malus_domestica MD12G1060500.v1.1 MD12G1060800.v1.1 MD13G1140200.v1.1 MD14G1060500.v1.1
prunus_persica Prupe.6G256200_v2.0.a1 Prupe.6G256200_v2.0.a1 Prupe.7G074000_v2.0.a1 Prupe.7G074000_v2.0.a1
pyrus_communis pycom14g04900
rosa_chinensis RchiOBHm_Chr2g0158881 RchiOBHm_Chr3g0476831 RchiOBHm_Chr3g0476851 RchiOBHm_Chr3g0476861 RchiOBHm_Chr3g0480221
rosa_laevigata RLG00000021120 RLG00000023509 RLG00000023748 RLG00000024125 RLG00000029770 RLG00000029771
rosa_multiflora Rmu_co8335633.1_g000001 Rmu_sc0002267.1_g000010 Rmu_sc0002679.1_g000001 Rmu_sc0006148.1_g000002 Rmu_sc0008085.1_g000009 Rmu_sc0009296.1_g000001 Rmu_sc0009296.1_g000015 Rmu_sc0014404.1_g000008 Rmu_sc0041425.1_g000001
rosa_roxburghii Rroxscaffold_2G00090920 Rroxscaffold_4G00319720 Rroxscaffold_4G00319850 Rroxscaffold_6G00401690 Rroxscaffold_6G00404530 Rroxscaffold_6G00404980 Rroxscaffold_6G00409500
rosa_rugosa Rorug02G0473000 Rorug03G0157100 Rorug03G0181200 Rorug03G0181300
rosa_samantha Rh1AG112100 Rh1CG109100 Rh1DG113400 Rh1DG117300 Rh2AG539100 Rh2BG551800 Rh2BG551900 Rh2CG522500 Rh2DG561600 Rh3AG171500 Rh3AG207000 Rh3AG207400 Rh3AG233000 Rh3BG197100 Rh3BG239900 Rh3BG240000 Rh3BG265600 Rh3CG188000 Rh3CG188100 Rh3CG233700 Rh3CG262800 Rh3DG177300 Rh3DG177400 Rh3DG233700 Rh3DG258800
rosa_wichuraiana Rw0G002320 Rw3G016860 Rw3G020460 Rw3G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 217, 313
AciI CCGC 3 cut(s) 47, 103, 147
AclWI GGATC 2 cut(s) 77, 284
AfaI GTAC 2 cut(s) 20, 367
AfiI CCNNNNNNNGG 2 cut(s) 92, 254
AgsI TTSAA 1 cut(s) 359
AjnI CCWGG 3 cut(s) 160, 196, 232
AluBI AGCT 1 cut(s) 173
AluI AGCT 1 cut(s) 173
AlwI GGATC 2 cut(s) 77, 284
AoxI GGCC 2 cut(s) 199, 235
ApeKI GCWGC 8 cut(s) 113, 116, 166, 173, 182, 185, 188, 457
BauI CACGAG 1 cut(s) 411
BbvI GCAGC 8 cut(s) 100, 103, 160, 169, 172, 175, 178, 469
BccI CCATC 1 cut(s) 377
BceAI ACGGC 2 cut(s) 134, 197
BciT130I CCWGG 3 cut(s) 162, 198, 234
BclI TGATCA 1 cut(s) 127
BfmI CTRYAG 1 cut(s) 402
BfuAI ACCTGC 2 cut(s) 217, 313
BisI GCNGC 8 cut(s) 114, 117, 167, 174, 183, 186, 189, 458
BlpI GCTNAGC 1 cut(s) 228
BlsI GCNGC 8 cut(s) 115, 118, 168, 175, 184, 187, 190, 459
BmcAI AGTACT 1 cut(s) 20
Bme1390I CCNGG 3 cut(s) 162, 198, 234
BmrFI CCNGG 3 cut(s) 162, 198, 234
BmsI GCATC 1 cut(s) 443
BpmI CTGGAG 1 cut(s) 548
Bpu1102I GCTNAGC 1 cut(s) 228
BsaAI YACGTR 1 cut(s) 369
BsaJI CCNNGG 3 cut(s) 161, 446, 461
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 254
Bse1I ACTGG 2 cut(s) 435, 565
Bse3DI GCAATG 3 cut(s) 177, 249, 519
BseBI CCWGG 3 cut(s) 162, 198, 234
BseDI CCNNGG 3 cut(s) 161, 446, 461
BseLI CCNNNNNNNGG 2 cut(s) 92, 254
BseMI GCAATG 3 cut(s) 177, 249, 519
BseMII CTCAG 2 cut(s) 60, 242
BseNI ACTGG 2 cut(s) 435, 565
BseRI GAGGAG 1 cut(s) 310
BseXI GCAGC 8 cut(s) 100, 103, 160, 169, 172, 175, 178, 469
BshFI GGCC 2 cut(s) 201, 237
BslI CCNNNNNNNGG 2 cut(s) 92, 254
BsmI GAATGC 1 cut(s) 141
BsnI GGCC 2 cut(s) 201, 237
Bsp143I GATC 3 cut(s) 82, 127, 289
Bsp1720I GCTNAGC 1 cut(s) 228
BspACI CCGC 3 cut(s) 47, 103, 147
BspANI GGCC 2 cut(s) 201, 237
BspCNI CTCAG 2 cut(s) 61, 241
BspMAI CTGCAG 1 cut(s) 406
BspMI ACCTGC 2 cut(s) 217, 313
BspPI GGATC 2 cut(s) 77, 284
BsrDI GCAATG 3 cut(s) 177, 249, 519
BsrI ACTGG 2 cut(s) 435, 565
BssECI CCNNGG 3 cut(s) 161, 446, 461
BssMI GATC 3 cut(s) 82, 127, 289
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 2 cut(s) 446, 461
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 3 cut(s) 162, 198, 234
Bst4CI ACNGT 3 cut(s) 34, 95, 223
Bst6I CTCTTC 1 cut(s) 498
BstAPI GCANNNNNTGC 1 cut(s) 440
BstBAI YACGTR 1 cut(s) 369
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 2 cut(s) 69, 228
BstKTI GATC 3 cut(s) 85, 130, 292
BstMBI GATC 3 cut(s) 82, 127, 289
BstMWI GCNNNNNNNGC 7 cut(s) 179, 182, 188, 224, 251, 260, 440
BstNI CCWGG 3 cut(s) 162, 198, 234
BstSCI CCNGG 3 cut(s) 160, 196, 232
BstSFI CTRYAG 1 cut(s) 402
BstV1I GCAGC 8 cut(s) 100, 103, 160, 169, 172, 175, 178, 469
BsuRI GGCC 2 cut(s) 201, 237
BveI ACCTGC 2 cut(s) 217, 313
Cac8I GCNNGC 1 cut(s) 171
Csp6I GTAC 2 cut(s) 19, 366
CviAII CATG 1 cut(s) 43
CviJI RGCY 8 cut(s) 59, 74, 173, 201, 232, 237, 245, 460
CviKI_1 RGCY 8 cut(s) 59, 74, 173, 201, 232, 237, 245, 460
CviQI GTAC 2 cut(s) 19, 366
DdeI CTNAG 2 cut(s) 69, 228
DpnI GATC 3 cut(s) 84, 129, 291
DpnII GATC 3 cut(s) 82, 127, 289
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
Eco130I CCWWGG 2 cut(s) 446, 461
EcoRII CCWGG 3 cut(s) 160, 196, 232
EcoT14I CCWWGG 2 cut(s) 446, 461
ErhI CCWWGG 2 cut(s) 446, 461
FaeI CATG 1 cut(s) 46
FaiI YATR 5 cut(s) 44, 56, 399, 482, 510
FatI CATG 1 cut(s) 42
FauI CCCGC 1 cut(s) 154
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 8 cut(s) 114, 117, 167, 174, 183, 186, 189, 458
Fsp4HI GCNGC 8 cut(s) 114, 117, 167, 174, 183, 186, 189, 458
GluI GCNGC 8 cut(s) 114, 117, 167, 174, 183, 186, 189, 458
GsuI CTGGAG 1 cut(s) 548
HaeIII GGCC 2 cut(s) 201, 237
Hin1II CATG 1 cut(s) 46
HincII GTYRAC 1 cut(s) 98
HindII GTYRAC 1 cut(s) 98
HinfI GANTC 1 cut(s) 407
Hpy166II GTNNAC 1 cut(s) 98
Hpy188I TCNGA 3 cut(s) 66, 132, 382
Hpy188III TCNNGA 2 cut(s) 293, 413
Hpy8I GTNNAC 1 cut(s) 98
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 2 cut(s) 283, 353
HpyCH4III ACNGT 3 cut(s) 34, 95, 223
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 2 cut(s) 404, 512
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 182, 188, 224, 251, 260, 440
HpyF3I CTNAG 2 cut(s) 69, 228
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 1 cut(s) 46
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 3 cut(s) 82, 127, 289
LmnI GCTCC 1 cut(s) 448
Lsp1109I GCAGC 8 cut(s) 100, 103, 160, 169, 172, 175, 178, 469
LweI GCATC 1 cut(s) 443
MaeII ACGT 1 cut(s) 368
MalI GATC 3 cut(s) 84, 129, 291
MboI GATC 3 cut(s) 82, 127, 289
MboII GAAGA 3 cut(s) 340, 515, 554
MluCI AATT 1 cut(s) 387
MnlI CCTC 2 cut(s) 64, 331
MspR9I CCNGG 3 cut(s) 162, 198, 234
Mva1269I GAATGC 1 cut(s) 141
MvaI CCWGG 3 cut(s) 162, 198, 234
MwoI GCNNNNNNNGC 7 cut(s) 179, 182, 188, 224, 251, 260, 440
NdeII GATC 3 cut(s) 82, 127, 289
NlaIII CATG 1 cut(s) 46
PctI GAATGC 1 cut(s) 141
PfeI GAWTC 1 cut(s) 407
PkrI GCNGC 8 cut(s) 115, 118, 168, 175, 184, 187, 190, 459
Ppu21I YACGTR 1 cut(s) 369
Psp6I CCWGG 3 cut(s) 160, 196, 232
PspGI CCWGG 3 cut(s) 160, 196, 232
PstI CTGCAG 1 cut(s) 406
RsaI GTAC 2 cut(s) 20, 367
RsaNI GTAC 2 cut(s) 19, 366
SatI GCNGC 8 cut(s) 114, 117, 167, 174, 183, 186, 189, 458
Sau3AI GATC 3 cut(s) 82, 127, 289
ScaI AGTACT 1 cut(s) 20
ScrFI CCNGG 3 cut(s) 162, 198, 234
SetI ASST 6 cut(s) 127, 175, 211, 307, 364, 371
SfaNI GCATC 1 cut(s) 443
SfcI CTRYAG 1 cut(s) 402
Sse9I AATT 1 cut(s) 387
SsiI CCGC 3 cut(s) 47, 103, 147
StyD4I CCNGG 3 cut(s) 160, 196, 232
StyI CCWWGG 2 cut(s) 446, 461
TaaI ACNGT 3 cut(s) 34, 95, 223
TaiI ACGT 1 cut(s) 371
TaqI TCGA 1 cut(s) 90
TasI AATT 1 cut(s) 387
TatI WGTACW 1 cut(s) 18
TfiI GAWTC 1 cut(s) 407
TseI GCWGC 8 cut(s) 113, 116, 166, 173, 182, 185, 188, 457
TspDTI ATGAA 6 cut(s) 31, 265, 386, 469, 510, 555
TspGWI ACGGA 1 cut(s) 349
ZrmI AGTACT 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.