Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000583

Physical Location & Seq

Genomic Coordinates Forward (+)

29170197 .. 29172293

Gene Structure 2097 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1203200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 954 bp

ATGGGAAAGTCTAGCGGCTATCCACCGGCGTCAGGCATAAAGAAAGGTTCATGGACCCCTGAAGAGGACAAAAGGCTTTTAGCTTACATTCAACTACACGGCCATGGAAGCTGGCGTTCCTTGCCCCAGAAAGCTGGTCTAAAAAGATGCGGGAAGAGCTGTAGGCTAAGGTGGAGAAACTACCTCAGACCTGATATTAAGAGGGGAAATTTCAGTTTGCACGAAGACCAAACCATCATTCAACTCCATGCACTTCTTGGCAACAGGTGGTCGGCCATAGCTGCACACTTACCAAAGAGAACAGACAATGAGATCAAGAACTACTGGAACGCAAATCTGAAGAAAAGGTTGGCCAAGATAGGATTTGACCCTGTAACCCACAAGTCCAAGGCTGCCATTCTTGCCTCTGCAAATGGCGACCCCAAAAAGTTGTCAAATCTCAGCCACATTGCTCAGTGGGAAAGTGCCAGGCTTCAAGCCGAGGCCAGGTTCGTCAAAGAATCCAAACTACGTATGCATGAATCTGCGCCGGCGGTGTCGTTTTTCGATGATTATCATCAGCTTGCTCCACCACTAGTTCCACAGTGCCTTGACATACTAGAAGCATCCGCATGGGAAAGCCTAATATCCATGTCAAAGTCATCGTCAAGATCCGCTGCCCTAACCAATGATATAAACGGCCTGGATCATCATCATGTTCATGACCATGGTCTTAATATGAACGGCCAAGATTATGGTCCTAATTTTCAGGTTCCAATATCTGATCAACAATTATCAAGCCTGATGGGATTTGATGAAGCAACGGCGCCATCAGTAGCCACTGGATCGATGAATATCAATGAACATTTCGGTCAATATTGTGGTAATGAATGCAGTGAATTATTGGTGGTCCTCCATCAAAATATCGGTTTTGAATCCGAAGATGCTTGGACTAGTTTGGAAAAATTAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

318

Amino Acids

35.4

Weight (kDa)

8.5

Isoelectric Point (pI)

48.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	15 - 62	2.1e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	18 - 78	2.4e-12	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	68 - 111	4.7e-16	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	72 - 123	6.4e-09	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000583)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01140 AT3G01140 AT5G15310 AT5G15310 AT5G15310 AT5G15310
fragaria_vesca	FvH4_4g31000 FvH4_5g14660 FvH4_5g14661 FvH4_6g48110
malus_domestica	MD00G1081800.v1.1 MD06G1196400.v1.1 MD06G1196500.v1.1 MD09G1054000.v1.1 MD14G1203200.v1.1 MD16G1069000.v1.1 MD17G1051700.v1.1
prunus_persica	Prupe.1G278200_v2.0.a1 Prupe.3G264500_v2.0.a1 Prupe.5G196100_v2.0.a1 Prupe.5G196100_v2.0.a1
pyrus_communis	pycom06g17330 pycom06g17350 pycom111g04520 pycom14g16820 pycom17g04890
rosa_chinensis	RchiOBHm_Chr2g0167441 RchiOBHm_Chr3g0485421 RchiOBHm_Chr4g0439371 RchiOBHm_Chr7g0181801 RchiOBHm_Chr7g0181821
rosa_laevigata	RLG00000005171 RLG00000021717 RLG00000023151
rosa_multiflora	Rmu_co8449019.1_g000001 Rmu_sc0000168.1_g000002 Rmu_sc0001120.1_g000006 Rmu_sc0001206.1_g000043 Rmu_sc0004898.1_g000006 Rmu_sc0005291.1_g000001 Rmu_sc0009036.1_g000001 Rmu_sc0010132.1_g000030 Rmu_sc0014780.1_g000004 Rmu_sc0028983.1_g000001
rosa_roxburghii	Rroxscaffold_3G00259110 Rroxscaffold_3G00271720 Rroxscaffold_5G00380300 Rroxscaffold_6G00397030
rosa_rugosa	Rorug02G0530400 Rorug03G0218000 Rorug04G0317800 Rorug06G0443600 Rorug06G0443700 Rorug06G0443900 Rorug06G0444100 Rorug06G0444300
rosa_samantha	Rh2AG597400 Rh2BG608800 Rh2CG579600 Rh2DG620000 Rh3AG267600 Rh3BG303000 Rh3DG296900 Rh3DG297800 Rh3DG298200 Rh4AG368900 Rh4BG380700 Rh4CG395200 Rh4DG375500 Rh7AG046200 Rh7AG046400 Rh7CG046500
rosa_wichuraiana	Rw2G049710 Rw3G023700 Rw3G023800 Rw3G023810 Rw3G023860 Rw3G023870 Rw4G031470 Rw7G003820 Rw7G003840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	805
AciI	CCGC	5 cut(s)	15, 150, 533, 609, 654
AclWI	GGATC	3 cut(s)	645, 693, 832
AcoI	YGGCCR	4 cut(s)	100, 273, 351, 724
AcsI	RAATTY	1 cut(s)	208
AcuI	CTGAAG	2 cut(s)	81, 359
AcyI	GRCGYC	2 cut(s)	29, 806
AfiI	CCNNNNNNNGG	3 cut(s)	32, 64, 486
AgsI	TTSAA	4 cut(s)	92, 242, 476, 914
AhlI	ACTAGT	2 cut(s)	574, 932
AjnI	CCWGG	3 cut(s)	467, 485, 681
AjuI	GAANNNNNNNTTGG	4 cut(s)	222, 254, 332, 364
AluBI	AGCT	6 cut(s)	83, 111, 134, 159, 281, 562
AluI	AGCT	6 cut(s)	83, 111, 134, 159, 281, 562
AlwI	GGATC	3 cut(s)	645, 693, 832
AoxI	GGCC	6 cut(s)	100, 273, 351, 483, 679, 724
ApeKI	GCWGC	3 cut(s)	281, 392, 656
ApoI	RAATTY	1 cut(s)	208
ArsI	GACNNNNNNTTYG	2 cut(s)	629, 661
AseI	ATTAAT	1 cut(s)	947
AspLEI	GCGC	2 cut(s)	529, 808
AspS9I	GGNCC	3 cut(s)	54, 737, 889
AvaII	GGWCC	3 cut(s)	54, 737, 889
BalI	TGGCCA	1 cut(s)	353
BanI	GGYRCC	1 cut(s)	805
BbsI	GAAGAC	1 cut(s)	231
BbvI	GCAGC	3 cut(s)	268, 379, 643
BccI	CCATC	4 cut(s)	242, 778, 817, 903
BceAI	ACGGC	4 cut(s)	115, 694, 739, 819
BciT130I	CCWGG	3 cut(s)	469, 487, 683
BclI	TGATCA	1 cut(s)	763
BcuI	ACTAGT	2 cut(s)	574, 932
BfaI	CTAG	4 cut(s)	12, 575, 599, 933
BfmI	CTRYAG	1 cut(s)	160
BfoI	RGCGCY	1 cut(s)	809
BisI	GCNGC	4 cut(s)	16, 282, 393, 657
BlsI	GCNGC	4 cut(s)	17, 283, 394, 658
Bme1390I	CCNGG	3 cut(s)	469, 487, 683
Bme18I	GGWCC	3 cut(s)	54, 737, 889
BmgT120I	GGNCC	3 cut(s)	54, 737, 889
BmiI	GGNNCC	3 cut(s)	56, 753, 807
BmrFI	CCNGG	3 cut(s)	469, 487, 683
BmsI	GCATC	3 cut(s)	137, 614, 913
BoxI	GACNNNNGTC	1 cut(s)	708
BpiI	GAAGAC	1 cut(s)	231
Bpu10I	CCTNAGC	1 cut(s)	167
Bsa29I	ATCGAT	1 cut(s)	827
BsaAI	YACGTR	1 cut(s)	512
BsaBI	GATNNNNATC	4 cut(s)	552, 555, 690, 833
BsaHI	GRCGYC	2 cut(s)	29, 806
BsaJI	CCNNGG	4 cut(s)	103, 387, 480, 706
Bsc4I	CCNNNNNNNGG	3 cut(s)	32, 64, 486
Bse118I	RCCGGY	2 cut(s)	25, 529
Bse1I	ACTGG	2 cut(s)	329, 826
Bse3DI	GCAATG	1 cut(s)	447
Bse8I	GATNNNNATC	4 cut(s)	552, 555, 690, 833
BseBI	CCWGG	3 cut(s)	469, 487, 683
BseCI	ATCGAT	1 cut(s)	827
BseDI	CCNNGG	4 cut(s)	103, 387, 480, 706
BseGI	GGATG	1 cut(s)	605
BseJI	GATNNNNATC	4 cut(s)	552, 555, 690, 833
BseLI	CCNNNNNNNGG	3 cut(s)	32, 64, 486
BseMI	GCAATG	1 cut(s)	447
BseMII	CTCAG	3 cut(s)	199, 454, 467
BseNI	ACTGG	2 cut(s)	329, 826
BseXI	GCAGC	3 cut(s)	268, 379, 643
BsgI	GTGCAG	1 cut(s)	267
BshFI	GGCC	6 cut(s)	102, 275, 353, 485, 681, 726
BshNI	GGYRCC	1 cut(s)	805
BshVI	ATCGAT	1 cut(s)	827
BsiSI	CCGG	2 cut(s)	26, 530
BslI	CCNNNNNNNGG	3 cut(s)	32, 64, 486
BsmI	GAATGC	1 cut(s)	875
BsnI	GGCC	6 cut(s)	102, 275, 353, 485, 681, 726
Bsp143I	GATC	5 cut(s)	312, 650, 685, 763, 824
Bsp19I	CCATGG	2 cut(s)	103, 706
BspACI	CCGC	5 cut(s)	15, 150, 533, 609, 654
BspANI	GGCC	6 cut(s)	102, 275, 353, 485, 681, 726
BspCNI	CTCAG	3 cut(s)	198, 453, 466
BspDI	ATCGAT	1 cut(s)	827
BspHI	TCATGA	1 cut(s)	700
BspLI	GGNNCC	3 cut(s)	56, 753, 807
BspPI	GGATC	3 cut(s)	645, 693, 832
BspQI	GCTCTTC	1 cut(s)	149
BspT107I	GGYRCC	1 cut(s)	805
BsrDI	GCAATG	1 cut(s)	447
BsrFI	RCCGGY	2 cut(s)	25, 529
BsrI	ACTGG	2 cut(s)	329, 826
BssAI	RCCGGY	2 cut(s)	25, 529
BssECI	CCNNGG	4 cut(s)	103, 387, 480, 706
BssMI	GATC	5 cut(s)	312, 650, 685, 763, 824
BssNI	GRCGYC	2 cut(s)	29, 806
BssT1I	CCWWGG	3 cut(s)	103, 387, 706
Bst2UI	CCWGG	3 cut(s)	469, 487, 683
Bst4CI	ACNGT	1 cut(s)	585
Bst6I	CTCTTC	2 cut(s)	57, 149
BstACI	GRCGYC	2 cut(s)	29, 806
BstBAI	YACGTR	1 cut(s)	512
BstC8I	GCNNGC	3 cut(s)	113, 531, 564
BstDEI	CTNAG	4 cut(s)	167, 185, 440, 453
BstDSI	CCRYGG	2 cut(s)	103, 706
BstF5I	GGATG	1 cut(s)	605
BstH2I	RGCGCY	1 cut(s)	809
BstHHI	GCGC	2 cut(s)	529, 808
BstKTI	GATC	5 cut(s)	315, 653, 688, 766, 827
BstMBI	GATC	5 cut(s)	312, 650, 685, 763, 824
BstMWI	GCNNNNNNNGC	5 cut(s)	108, 121, 156, 281, 401
BstNI	CCWGG	3 cut(s)	469, 487, 683
BstPAI	GACNNNNGTC	1 cut(s)	708
BstSCI	CCNGG	3 cut(s)	467, 485, 681
BstSFI	CTRYAG	1 cut(s)	160
BstSNI	TACGTA	1 cut(s)	512
BstV1I	GCAGC	3 cut(s)	268, 379, 643
BstV2I	GAAGAC	1 cut(s)	231
BstX2I	RGATCY	1 cut(s)	650
BstXI	CCANNNNNNTGG	2 cut(s)	134, 734
BstYI	RGATCY	1 cut(s)	650
Bsu15I	ATCGAT	1 cut(s)	827
BsuRI	GGCC	6 cut(s)	102, 275, 353, 485, 681, 726
BsuTUI	ATCGAT	1 cut(s)	827
BtgI	CCRYGG	2 cut(s)	103, 706
BtsCI	GGATG	1 cut(s)	605
BtsI	GCAGTG	1 cut(s)	880
BtsIMutI	CAGTG	4 cut(s)	461, 590, 819, 880
Cac8I	GCNNGC	3 cut(s)	113, 531, 564
CciI	TCATGA	1 cut(s)	700
CfoI	GCGC	2 cut(s)	529, 808
Cfr10I	RCCGGY	2 cut(s)	25, 529
Cfr13I	GGNCC	3 cut(s)	54, 737, 889
ClaI	ATCGAT	1 cut(s)	827
CseI	GACGC	1 cut(s)	18
CviAII	CATG	9 cut(s)	51, 104, 248, 518, 612, 631, 695, 701, 707
DdeI	CTNAG	4 cut(s)	167, 185, 440, 453
DinI	GGCGCC	1 cut(s)	807
DpnI	GATC	5 cut(s)	314, 652, 687, 765, 826
DpnII	GATC	5 cut(s)	312, 650, 685, 763, 824
EaeI	YGGCCR	4 cut(s)	100, 273, 351, 724
Eam1104I	CTCTTC	2 cut(s)	57, 149
EarI	CTCTTC	2 cut(s)	57, 149
Eco105I	TACGTA	1 cut(s)	512
Eco130I	CCWWGG	3 cut(s)	103, 387, 706
Eco47I	GGWCC	3 cut(s)	54, 737, 889
Eco57I	CTGAAG	2 cut(s)	81, 359
EcoRII	CCWGG	3 cut(s)	467, 485, 681
EcoT14I	CCWWGG	3 cut(s)	103, 387, 706
EcoT22I	ATGCAT	1 cut(s)	519
EgeI	GGCGCC	1 cut(s)	807
EheI	GGCGCC	1 cut(s)	807
ErhI	CCWWGG	3 cut(s)	103, 387, 706
FaeI	CATG	9 cut(s)	54, 107, 251, 521, 615, 634, 698, 704, 710
FatI	CATG	9 cut(s)	50, 103, 247, 517, 611, 630, 694, 700, 706
FauI	CCCGC	1 cut(s)	143
FbaI	TGATCA	1 cut(s)	763
Fnu4HI	GCNGC	4 cut(s)	16, 282, 393, 657
FokI	GGATG	1 cut(s)	592
Fsp4HI	GCNGC	4 cut(s)	16, 282, 393, 657
FspBI	CTAG	4 cut(s)	12, 575, 599, 933
GlaI	GCGC	2 cut(s)	528, 807
GluI	GCNGC	4 cut(s)	16, 282, 393, 657
HaeII	RGCGCY	1 cut(s)	809
HaeIII	GGCC	6 cut(s)	102, 275, 353, 485, 681, 726
HapII	CCGG	2 cut(s)	26, 530
HgaI	GACGC	1 cut(s)	18
HhaI	GCGC	2 cut(s)	529, 808
Hin1I	GRCGYC	2 cut(s)	29, 806
Hin1II	CATG	9 cut(s)	54, 107, 251, 521, 615, 634, 698, 704, 710
Hin6I	GCGC	2 cut(s)	527, 806
HinP1I	GCGC	2 cut(s)	527, 806
HinfI	GANTC	3 cut(s)	500, 521, 914
HpaII	CCGG	2 cut(s)	26, 530
Hpy188I	TCNGA	4 cut(s)	188, 339, 763, 919
Hpy188III	TCNNGA	3 cut(s)	316, 648, 701
HpyCH4III	ACNGT	1 cut(s)	585
HpyCH4IV	ACGT	1 cut(s)	511
HpyCH4V	TGCA	6 cut(s)	220, 251, 284, 410, 517, 873
HpyF10VI	GCNNNNNNNGC	5 cut(s)	108, 121, 156, 281, 401
HpyF3I	CTNAG	4 cut(s)	167, 185, 440, 453
HpySE526I	ACGT	1 cut(s)	511
Hsp92I	GRCGYC	2 cut(s)	29, 806
Hsp92II	CATG	9 cut(s)	54, 107, 251, 521, 615, 634, 698, 704, 710
HspAI	GCGC	2 cut(s)	527, 806
KasI	GGCGCC	1 cut(s)	805
KroI	GCCGGC	1 cut(s)	529
KroNI	GCCGGC	1 cut(s)	531
Ksp22I	TGATCA	1 cut(s)	763
Kzo9I	GATC	5 cut(s)	312, 650, 685, 763, 824
LguI	GCTCTTC	1 cut(s)	149
LmnI	GCTCC	1 cut(s)	571
Lsp1109I	GCAGC	3 cut(s)	268, 379, 643
LweI	GCATC	3 cut(s)	137, 614, 913
MaeI	CTAG	4 cut(s)	12, 575, 599, 933
MaeII	ACGT	1 cut(s)	511
MaeIII	GTNAC	1 cut(s)	373
MalI	GATC	5 cut(s)	314, 652, 687, 765, 826
MboI	GATC	5 cut(s)	312, 650, 685, 763, 824
MboII	GAAGA	5 cut(s)	74, 166, 236, 352, 932
MflI	RGATCY	1 cut(s)	650
MlsI	TGGCCA	1 cut(s)	353
MluCI	AATT	5 cut(s)	208, 742, 770, 878, 944
MluNI	TGGCCA	1 cut(s)	353
Mly113I	GGCGCC	1 cut(s)	806
MnlI	CCTC	6 cut(s)	58, 194, 195, 415, 475, 902
Mox20I	TGGCCA	1 cut(s)	353
Mph1103I	ATGCAT	1 cut(s)	519
MreI	CGCCGGCG	1 cut(s)	529
MroNI	GCCGGC	1 cut(s)	529
MscI	TGGCCA	1 cut(s)	353
MseI	TTAA	3 cut(s)	198, 714, 947
MslI	CAYNNNNRTG	5 cut(s)	102, 610, 693, 699, 705
Msp20I	TGGCCA	1 cut(s)	353
MspA1I	CMGCKG	1 cut(s)	656
MspI	CCGG	2 cut(s)	26, 530
MspR9I	CCNGG	3 cut(s)	469, 487, 683
Mva1269I	GAATGC	1 cut(s)	875
MvaI	CCWGG	3 cut(s)	469, 487, 683
MwoI	GCNNNNNNNGC	5 cut(s)	108, 121, 156, 281, 401
NaeI	GCCGGC	1 cut(s)	531
NarI	GGCGCC	1 cut(s)	806
NcoI	CCATGG	2 cut(s)	103, 706
NdeII	GATC	5 cut(s)	312, 650, 685, 763, 824
NgoMIV	GCCGGC	1 cut(s)	529
NlaIII	CATG	9 cut(s)	54, 107, 251, 521, 615, 634, 698, 704, 710
NlaIV	GGNNCC	3 cut(s)	56, 753, 807
NmeAIII	GCCGAG	1 cut(s)	505
NsiI	ATGCAT	1 cut(s)	519
PagI	TCATGA	1 cut(s)	700
PciSI	GCTCTTC	1 cut(s)	149
PctI	GAATGC	1 cut(s)	875
PdiI	GCCGGC	1 cut(s)	531
PfeI	GAWTC	3 cut(s)	500, 521, 914
PkrI	GCNGC	4 cut(s)	17, 283, 394, 658
PluTI	GGCGCC	1 cut(s)	809
Ppu21I	YACGTR	1 cut(s)	512
PshAI	GACNNNNGTC	1 cut(s)	708
PshBI	ATTAAT	1 cut(s)	947
Psp6I	CCWGG	3 cut(s)	467, 485, 681
PspGI	CCWGG	3 cut(s)	467, 485, 681
PspN4I	GGNNCC	3 cut(s)	56, 753, 807
PspPI	GGNCC	3 cut(s)	54, 737, 889
PsuI	RGATCY	1 cut(s)	650
RseI	CAYNNNNRTG	5 cut(s)	102, 610, 693, 699, 705
SapI	GCTCTTC	1 cut(s)	149
SaqAI	TTAA	3 cut(s)	198, 714, 947
SatI	GCNGC	4 cut(s)	16, 282, 393, 657
Sau3AI	GATC	5 cut(s)	312, 650, 685, 763, 824
Sau96I	GGNCC	3 cut(s)	54, 737, 889
ScrFI	CCNGG	3 cut(s)	469, 487, 683
SfaNI	GCATC	3 cut(s)	137, 614, 913
SfcI	CTRYAG	1 cut(s)	160
SfoI	GGCGCC	1 cut(s)	807
SgrAI	CRCCGGYG	2 cut(s)	25, 529
SinI	GGWCC	3 cut(s)	54, 737, 889
SmiMI	CAYNNNNRTG	5 cut(s)	102, 610, 693, 699, 705
SnaBI	TACGTA	1 cut(s)	512
SpeI	ACTAGT	2 cut(s)	574, 932
Sse9I	AATT	5 cut(s)	208, 742, 770, 878, 944
SsiI	CCGC	5 cut(s)	15, 150, 533, 609, 654
SspDI	GGCGCC	1 cut(s)	805
SspI	AATATT	1 cut(s)	857
SspMI	CTAG	4 cut(s)	12, 575, 599, 933
StyD4I	CCNGG	3 cut(s)	467, 485, 681
StyI	CCWWGG	3 cut(s)	103, 387, 706
TaaI	ACNGT	1 cut(s)	585
TaiI	ACGT	1 cut(s)	514
TaqI	TCGA	2 cut(s)	546, 827
TaqII	GACCGA	1 cut(s)	839
TasI	AATT	5 cut(s)	208, 742, 770, 878, 944
TauI	GCSGC	1 cut(s)	18
TfiI	GAWTC	3 cut(s)	500, 521, 914
Tru1I	TTAA	3 cut(s)	198, 714, 947
Tru9I	TTAA	3 cut(s)	198, 714, 947
TscAI	CASTG	4 cut(s)	461, 590, 826, 880
TseI	GCWGC	3 cut(s)	281, 392, 656
TspDTI	ATGAA	8 cut(s)	39, 534, 689, 734, 810, 845, 855, 882
TspRI	CASTG	4 cut(s)	461, 590, 826, 880
VpaK11BI	GGWCC	3 cut(s)	54, 737, 889
VspI	ATTAAT	1 cut(s)	947
XapI	RAATTY	1 cut(s)	208
XcmI	CCANNNNNNNNNTGG	1 cut(s)	254
XspI	CTAG	4 cut(s)	12, 575, 599, 933
Zsp2I	ATGCAT	1 cut(s)	519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.