Transcription factor
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 858 |
| AccI | GTMKAC | 1 cut(s) | 649 |
| AccIII | TCCGGA | 1 cut(s) | 688 |
| AciI | CCGC | 4 cut(s) | 127, 405, 546, 858 |
| AclI | AACGTT | 1 cut(s) | 17 |
| AclWI | GGATC | 1 cut(s) | 374 |
| AcoI | YGGCCR | 1 cut(s) | 112 |
| AcsI | RAATTY | 1 cut(s) | 220 |
| AfaI | GTAC | 2 cut(s) | 633, 721 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 372, 745 |
| AgsI | TTSAA | 5 cut(s) | 104, 488, 599, 697, 868 |
| AlwI | GGATC | 1 cut(s) | 374 |
| Ama87I | CYCGRG | 1 cut(s) | 137 |
| Aor13HI | TCCGGA | 1 cut(s) | 688 |
| AoxI | GGCC | 2 cut(s) | 59, 112 |
| ApeKI | GCWGC | 4 cut(s) | 171, 293, 539, 683 |
| ApoI | RAATTY | 1 cut(s) | 220 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 428, 460 |
| AspS9I | GGNCC | 2 cut(s) | 59, 129 |
| AsuHPI | GGTGA | 2 cut(s) | 584, 746 |
| AvaI | CYCGRG | 1 cut(s) | 137 |
| AvaII | GGWCC | 1 cut(s) | 129 |
| BalI | TGGCCA | 1 cut(s) | 114 |
| BbsI | GAAGAC | 1 cut(s) | 243 |
| BbvCI | CCTCAGC | 1 cut(s) | 452 |
| BbvI | GCAGC | 4 cut(s) | 158, 280, 526, 695 |
| BccI | CCATC | 4 cut(s) | 177, 254, 416, 569 |
| BclI | TGATCA | 1 cut(s) | 849 |
| BfaI | CTAG | 2 cut(s) | 480, 498 |
| BfmI | CTRYAG | 2 cut(s) | 172, 540 |
| BisI | GCNGC | 4 cut(s) | 172, 294, 540, 684 |
| BlsI | GCNGC | 4 cut(s) | 173, 295, 541, 685 |
| Bme18I | GGWCC | 1 cut(s) | 129 |
| BmeT110I | CYCGRG | 1 cut(s) | 137 |
| BmgT120I | GGNCC | 2 cut(s) | 59, 129 |
| BmiI | GGNNCC | 2 cut(s) | 514, 663 |
| BmrI | ACTGGG | 1 cut(s) | 789 |
| BmsI | GCATC | 1 cut(s) | 787 |
| BmuI | ACTGGG | 1 cut(s) | 789 |
| BpiI | GAAGAC | 1 cut(s) | 243 |
| BpmI | CTGGAG | 1 cut(s) | 743 |
| Bpu10I | CCTNAGC | 1 cut(s) | 452 |
| BpuEI | CTTGAG | 1 cut(s) | 57 |
| BsaJI | CCNNGG | 3 cut(s) | 62, 115, 305 |
| BsaWI | WCCGGW | 1 cut(s) | 688 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 741, 771 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 372, 745 |
| Bse1I | ACTGG | 3 cut(s) | 341, 784, 886 |
| BseAI | TCCGGA | 1 cut(s) | 688 |
| BseDI | CCNNGG | 3 cut(s) | 62, 115, 305 |
| BseGI | GGATG | 2 cut(s) | 408, 802 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 372, 745 |
| BseMII | CTCAG | 1 cut(s) | 466 |
| BseNI | ACTGG | 3 cut(s) | 341, 784, 886 |
| BseRI | GAGGAG | 2 cut(s) | 60, 725 |
| BseXI | GCAGC | 4 cut(s) | 158, 280, 526, 695 |
| BsgI | GTGCAG | 1 cut(s) | 852 |
| BshFI | GGCC | 2 cut(s) | 61, 114 |
| BsiHKCI | CYCGRG | 1 cut(s) | 137 |
| BsiSI | CCGG | 1 cut(s) | 689 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 372, 745 |
| BsnI | GGCC | 2 cut(s) | 61, 114 |
| BsoBI | CYCGRG | 1 cut(s) | 137 |
| Bsp13I | TCCGGA | 1 cut(s) | 688 |
| Bsp143I | GATC | 2 cut(s) | 379, 849 |
| Bsp19I | CCATGG | 2 cut(s) | 62, 115 |
| BspACI | CCGC | 4 cut(s) | 127, 405, 546, 858 |
| BspANI | GGCC | 2 cut(s) | 61, 114 |
| BspCNI | CTCAG | 1 cut(s) | 465 |
| BspEI | TCCGGA | 1 cut(s) | 688 |
| BspHI | TCATGA | 1 cut(s) | 852 |
| BspLI | GGNNCC | 2 cut(s) | 514, 663 |
| BspMAI | CTGCAG | 2 cut(s) | 176, 544 |
| BspPI | GGATC | 1 cut(s) | 374 |
| BspQI | GCTCTTC | 1 cut(s) | 161 |
| BsrBI | CCGCTC | 1 cut(s) | 858 |
| BsrI | ACTGG | 3 cut(s) | 341, 784, 886 |
| BssECI | CCNNGG | 3 cut(s) | 62, 115, 305 |
| BssMI | GATC | 2 cut(s) | 379, 849 |
| BssT1I | CCWWGG | 3 cut(s) | 62, 115, 305 |
| Bst4CI | ACNGT | 4 cut(s) | 468, 629, 654, 734 |
| Bst6I | CTCTTC | 2 cut(s) | 161, 350 |
| BstC8I | GCNNGC | 3 cut(s) | 125, 176, 544 |
| BstDEI | CTNAG | 2 cut(s) | 179, 452 |
| BstDSI | CCRYGG | 2 cut(s) | 62, 115 |
| BstEII | GGTNACC | 1 cut(s) | 734 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 743 |
| BstF5I | GGATG | 2 cut(s) | 408, 802 |
| BstKTI | GATC | 2 cut(s) | 382, 852 |
| BstMBI | GATC | 2 cut(s) | 379, 849 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 120, 133, 293, 413 |
| BstPI | GGTNACC | 1 cut(s) | 734 |
| BstSFI | CTRYAG | 2 cut(s) | 172, 540 |
| BstV1I | GCAGC | 4 cut(s) | 158, 280, 526, 695 |
| BstV2I | GAAGAC | 1 cut(s) | 243 |
| BsuRI | GGCC | 2 cut(s) | 61, 114 |
| BtgI | CCRYGG | 2 cut(s) | 62, 115 |
| BtgZI | GCGATG | 1 cut(s) | 573 |
| BtsCI | GGATG | 2 cut(s) | 408, 802 |
| BtsI | GCAGTG | 1 cut(s) | 30 |
| BtsIMutI | CAGTG | 3 cut(s) | 30, 473, 835 |
| Cac8I | GCNNGC | 3 cut(s) | 125, 176, 544 |
| CciI | TCATGA | 1 cut(s) | 852 |
| Cfr13I | GGNCC | 2 cut(s) | 59, 129 |
| Csp6I | GTAC | 2 cut(s) | 632, 720 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 574, 609 |
| CviAII | CATG | 6 cut(s) | 63, 110, 116, 260, 508, 853 |
| CviQI | GTAC | 2 cut(s) | 632, 720 |
| DdeI | CTNAG | 2 cut(s) | 179, 452 |
| DpnI | GATC | 2 cut(s) | 381, 851 |
| DpnII | GATC | 2 cut(s) | 379, 849 |
| EaeI | YGGCCR | 1 cut(s) | 112 |
| Eam1104I | CTCTTC | 2 cut(s) | 161, 350 |
| EarI | CTCTTC | 2 cut(s) | 161, 350 |
| Eco130I | CCWWGG | 3 cut(s) | 62, 115, 305 |
| Eco32I | GATATC | 1 cut(s) | 826 |
| Eco47I | GGWCC | 1 cut(s) | 129 |
| Eco88I | CYCGRG | 1 cut(s) | 137 |
| Eco91I | GGTNACC | 1 cut(s) | 734 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 743 |
| EcoO65I | GGTNACC | 1 cut(s) | 734 |
| EcoRV | GATATC | 1 cut(s) | 826 |
| EcoT14I | CCWWGG | 3 cut(s) | 62, 115, 305 |
| ErhI | CCWWGG | 3 cut(s) | 62, 115, 305 |
| FaeI | CATG | 6 cut(s) | 66, 113, 119, 263, 511, 856 |
| FaiI | YATR | 9 cut(s) | 64, 111, 117, 261, 290, 461, 509, 854, 881 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 74, 106 |
| FatI | CATG | 6 cut(s) | 62, 109, 115, 259, 507, 852 |
| FauI | CCCGC | 1 cut(s) | 851 |
| FbaI | TGATCA | 1 cut(s) | 849 |
| FblI | GTMKAC | 1 cut(s) | 649 |
| Fnu4HI | GCNGC | 4 cut(s) | 172, 294, 540, 684 |
| FokI | GGATG | 2 cut(s) | 395, 809 |
| Fsp4HI | GCNGC | 4 cut(s) | 172, 294, 540, 684 |
| FspBI | CTAG | 2 cut(s) | 480, 498 |
| GluI | GCNGC | 4 cut(s) | 172, 294, 540, 684 |
| GsuI | CTGGAG | 1 cut(s) | 743 |
| HaeIII | GGCC | 2 cut(s) | 61, 114 |
| HapII | CCGG | 1 cut(s) | 689 |
| Hin1II | CATG | 6 cut(s) | 66, 113, 119, 263, 511, 856 |
| HincII | GTYRAC | 1 cut(s) | 21 |
| HindII | GTYRAC | 1 cut(s) | 21 |
| HinfI | GANTC | 1 cut(s) | 67 |
| HpaI | GTTAAC | 1 cut(s) | 21 |
| HpaII | CCGG | 1 cut(s) | 689 |
| HphI | GGTGA | 2 cut(s) | 584, 746 |
| Hpy166II | GTNNAC | 4 cut(s) | 21, 152, 632, 650 |
| Hpy188I | TCNGA | 2 cut(s) | 46, 709 |
| Hpy188III | TCNNGA | 5 cut(s) | 12, 74, 599, 689, 853 |
| Hpy8I | GTNNAC | 4 cut(s) | 21, 152, 632, 650 |
| HpyCH4III | ACNGT | 4 cut(s) | 468, 629, 654, 734 |
| HpyCH4IV | ACGT | 1 cut(s) | 17 |
| HpyCH4V | TGCA | 5 cut(s) | 174, 232, 263, 542, 833 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 120, 133, 293, 413 |
| HpyF3I | CTNAG | 2 cut(s) | 179, 452 |
| HpySE526I | ACGT | 1 cut(s) | 17 |
| Hsp92II | CATG | 6 cut(s) | 66, 113, 119, 263, 511, 856 |
| Kpn2I | TCCGGA | 1 cut(s) | 688 |
| Ksp22I | TGATCA | 1 cut(s) | 849 |
| KspAI | GTTAAC | 1 cut(s) | 21 |
| Kzo9I | GATC | 2 cut(s) | 379, 849 |
| LguI | GCTCTTC | 1 cut(s) | 161 |
| LmnI | GCTCC | 2 cut(s) | 667, 691 |
| Lsp1109I | GCAGC | 4 cut(s) | 158, 280, 526, 695 |
| LweI | GCATC | 1 cut(s) | 787 |
| MaeI | CTAG | 2 cut(s) | 480, 498 |
| MaeII | ACGT | 1 cut(s) | 17 |
| MaeIII | GTNAC | 2 cut(s) | 385, 734 |
| MalI | GATC | 2 cut(s) | 381, 851 |
| MbiI | CCGCTC | 1 cut(s) | 858 |
| MboI | GATC | 2 cut(s) | 379, 849 |
| MboII | GAAGA | 3 cut(s) | 178, 248, 367 |
| MlsI | TGGCCA | 1 cut(s) | 114 |
| MluCI | AATT | 3 cut(s) | 220, 619, 668 |
| MluNI | TGGCCA | 1 cut(s) | 114 |
| MlyI | GAGTC | 1 cut(s) | 61 |
| Mox20I | TGGCCA | 1 cut(s) | 114 |
| MroI | TCCGGA | 1 cut(s) | 688 |
| MscI | TGGCCA | 1 cut(s) | 114 |
| MseI | TTAA | 4 cut(s) | 20, 210, 504, 671 |
| MslI | CAYNNNNRTG | 1 cut(s) | 114 |
| Msp20I | TGGCCA | 1 cut(s) | 114 |
| MspI | CCGG | 1 cut(s) | 689 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 120, 133, 293, 413 |
| NcoI | CCATGG | 2 cut(s) | 62, 115 |
| NdeII | GATC | 2 cut(s) | 379, 849 |
| NlaIII | CATG | 6 cut(s) | 66, 113, 119, 263, 511, 856 |
| NlaIV | GGNNCC | 2 cut(s) | 514, 663 |
| NmeAIII | GCCGAG | 1 cut(s) | 249 |
| NmuCI | GTSAC | 1 cut(s) | 734 |
| PagI | TCATGA | 1 cut(s) | 852 |
| PciSI | GCTCTTC | 1 cut(s) | 161 |
| PkrI | GCNGC | 4 cut(s) | 173, 295, 541, 685 |
| PleI | GAGTC | 1 cut(s) | 61 |
| PpsI | GAGTC | 1 cut(s) | 61 |
| Psp1406I | AACGTT | 1 cut(s) | 17 |
| PspEI | GGTNACC | 1 cut(s) | 734 |
| PspN4I | GGNNCC | 2 cut(s) | 514, 663 |
| PspPI | GGNCC | 2 cut(s) | 59, 129 |
| PstI | CTGCAG | 2 cut(s) | 176, 544 |
| RsaI | GTAC | 2 cut(s) | 633, 721 |
| RsaNI | GTAC | 2 cut(s) | 632, 720 |
| RseI | CAYNNNNRTG | 1 cut(s) | 114 |
| SapI | GCTCTTC | 1 cut(s) | 161 |
| SaqAI | TTAA | 4 cut(s) | 20, 210, 504, 671 |
| SatI | GCNGC | 4 cut(s) | 172, 294, 540, 684 |
| Sau3AI | GATC | 2 cut(s) | 379, 849 |
| Sau96I | GGNCC | 2 cut(s) | 59, 129 |
| SchI | GAGTC | 1 cut(s) | 61 |
| SfaNI | GCATC | 1 cut(s) | 787 |
| SfcI | CTRYAG | 2 cut(s) | 172, 540 |
| SinI | GGWCC | 1 cut(s) | 129 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 114 |
| SmlI | CTYRAG | 1 cut(s) | 72 |
| SmoI | CTYRAG | 1 cut(s) | 72 |
| Sse9I | AATT | 3 cut(s) | 220, 619, 668 |
| SsiI | CCGC | 4 cut(s) | 127, 405, 546, 858 |
| SspMI | CTAG | 2 cut(s) | 480, 498 |
| StyI | CCWWGG | 3 cut(s) | 62, 115, 305 |
| TaaI | ACNGT | 4 cut(s) | 468, 629, 654, 734 |
| TaiI | ACGT | 1 cut(s) | 20 |
| TasI | AATT | 3 cut(s) | 220, 619, 668 |
| TatI | WGTACW | 1 cut(s) | 631 |
| Tru1I | TTAA | 4 cut(s) | 20, 210, 504, 671 |
| Tru9I | TTAA | 4 cut(s) | 20, 210, 504, 671 |
| TscAI | CASTG | 3 cut(s) | 37, 473, 835 |
| TseFI | GTSAC | 1 cut(s) | 734 |
| TseI | GCWGC | 4 cut(s) | 171, 293, 539, 683 |
| Tsp45I | GTSAC | 1 cut(s) | 734 |
| TspDTI | ATGAA | 5 cut(s) | 88, 248, 576, 600, 741 |
| TspRI | CASTG | 3 cut(s) | 37, 473, 835 |
| VpaK11BI | GGWCC | 1 cut(s) | 129 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 743 |
| XapI | RAATTY | 1 cut(s) | 220 |
| XmiI | GTMKAC | 1 cut(s) | 649 |
| XspI | CTAG | 2 cut(s) | 480, 498 |