Transcription factor
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Peripla_BP_6 | PF13458 | 39 - 340 | 4.5e-14 | Periplasmic binding protein |
| ANF_receptor | PF01094 | 56 - 409 | 2.6e-81 | Receptor family ligand binding region |
| Lig_chan-Glu_bd | PF10613 | 483 - 580 | 1.2e-13 | Ligated ion channel L-glutamate- and glycine-binding site |
| SBP_bac_3 | PF00497 | 493 - 819 | 5.7e-10 | Bacterial extracellular solute-binding proteins, family 3 |
| Lig_chan | PF00060 | 597 - 852 | 9.5e-41 | Ligand-gated ion channel |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 353 |
| AatII | GACGTC | 1 cut(s) | 594 |
| Acc36I | ACCTGC | 1 cut(s) | 457 |
| AccBSI | CCGCTC | 2 cut(s) | 426, 449 |
| AccI | GTMKAC | 2 cut(s) | 531, 588 |
| AccII | CGCG | 3 cut(s) | 77, 444, 2385 |
| AciI | CCGC | 9 cut(s) | 77, 86, 396, 424, 442, 447, 819, 2031, 2252 |
| AclI | AACGTT | 1 cut(s) | 2330 |
| AclWI | GGATC | 1 cut(s) | 1803 |
| AcoI | YGGCCR | 3 cut(s) | 78, 669, 999 |
| AcsI | RAATTY | 4 cut(s) | 1060, 1144, 1285, 2664 |
| AcuI | CTGAAG | 3 cut(s) | 1190, 2120, 2787 |
| AcyI | GRCGYC | 1 cut(s) | 591 |
| AdeI | CACNNNGTG | 1 cut(s) | 2779 |
| AfaI | GTAC | 4 cut(s) | 434, 615, 718, 877 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 607, 713, 1265, 1397, 2813 |
| AflIII | ACRYGT | 3 cut(s) | 174, 699, 798 |
| AgeI | ACCGGT | 1 cut(s) | 1677 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 515 |
| AhlI | ACTAGT | 2 cut(s) | 1120, 1328 |
| AjnI | CCWGG | 4 cut(s) | 218, 921, 934, 1351 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1614, 1646 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2435, 2467 |
| Alw21I | GWGCWC | 1 cut(s) | 888 |
| Alw26I | GTCTC | 4 cut(s) | 183, 1350, 2261, 2500 |
| AlwI | GGATC | 1 cut(s) | 1803 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 272, 1718 |
| Ama87I | CYCGRG | 3 cut(s) | 887, 2516, 2737 |
| ApeKI | GCWGC | 9 cut(s) | 165, 223, 269, 272, 275, 986, 1186, 1646, 2255 |
| ApoI | RAATTY | 4 cut(s) | 1060, 1144, 1285, 2664 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 524, 556 |
| AsiGI | ACCGGT | 1 cut(s) | 1677 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1626, 2220 |
| AspLEI | GCGC | 2 cut(s) | 446, 2773 |
| AspS9I | GGNCC | 7 cut(s) | 283, 320, 609, 627, 1883, 2187, 2530 |
| AsuC2I | CCSGG | 2 cut(s) | 608, 2548 |
| AsuHPI | GGTGA | 9 cut(s) | 115, 237, 1069, 1252, 1595, 1886, 2364, 2435, 2828 |
| AsuII | TTCGAA | 1 cut(s) | 1515 |
| AvaI | CYCGRG | 3 cut(s) | 887, 2516, 2737 |
| AvaII | GGWCC | 2 cut(s) | 283, 2187 |
| BaeGI | GKGCMC | 2 cut(s) | 487, 1740 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 708, 741 |
| BalI | TGGCCA | 2 cut(s) | 671, 1001 |
| BanII | GRGCYC | 2 cut(s) | 888, 979 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2079, 2111 |
| BbsI | GAAGAC | 7 cut(s) | 52, 679, 933, 1106, 1504, 1878, 2350 |
| Bbv12I | GWGCWC | 1 cut(s) | 888 |
| BbvI | GCAGC | 9 cut(s) | 152, 210, 256, 259, 287, 973, 1198, 1633, 2242 |
| BceAI | ACGGC | 3 cut(s) | 65, 156, 596 |
| BciT130I | CCWGG | 4 cut(s) | 220, 923, 936, 1353 |
| BciVI | GTATCC | 2 cut(s) | 1490, 1735 |
| BclI | TGATCA | 2 cut(s) | 12, 1986 |
| BcnI | CCSGG | 2 cut(s) | 608, 2548 |
| BcoDI | GTCTC | 4 cut(s) | 183, 1350, 2261, 2500 |
| BcuI | ACTAGT | 2 cut(s) | 1120, 1328 |
| BfaI | CTAG | 7 cut(s) | 66, 720, 1121, 1329, 1853, 1974, 2162 |
| BfmI | CTRYAG | 1 cut(s) | 273 |
| BfuAI | ACCTGC | 1 cut(s) | 457 |
| BfuI | GTATCC | 2 cut(s) | 1490, 1735 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 86 |
| BglII | AGATCT | 1 cut(s) | 1657 |
| BlpI | GCTNAGC | 1 cut(s) | 161 |
| Bme1390I | CCNGG | 6 cut(s) | 220, 608, 923, 936, 1353, 2548 |
| Bme18I | GGWCC | 2 cut(s) | 283, 2187 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 515 |
| BmeT110I | CYCGRG | 3 cut(s) | 887, 2516, 2737 |
| BmgT120I | GGNCC | 7 cut(s) | 283, 320, 609, 627, 1883, 2187, 2530 |
| BmiI | GGNNCC | 1 cut(s) | 2531 |
| BmrFI | CCNGG | 6 cut(s) | 220, 608, 923, 936, 1353, 2548 |
| BmsI | GCATC | 4 cut(s) | 177, 562, 973, 1633 |
| BoxI | GACNNNNGTC | 1 cut(s) | 683 |
| BpiI | GAAGAC | 7 cut(s) | 52, 679, 933, 1106, 1504, 1878, 2350 |
| BpmI | CTGGAG | 1 cut(s) | 1374 |
| Bpu10I | CCTNAGC | 3 cut(s) | 738, 2183, 2808 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 161 |
| Bpu14I | TTCGAA | 1 cut(s) | 1515 |
| BpuMI | CCSGG | 2 cut(s) | 608, 2548 |
| BsaAI | YACGTR | 1 cut(s) | 529 |
| BsaHI | GRCGYC | 1 cut(s) | 591 |
| BsaI | GGTCTC | 1 cut(s) | 2261 |
| BsaWI | WCCGGW | 2 cut(s) | 100, 1677 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 105, 135, 1224, 1254, 2788, 2818 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 607, 713, 1265, 1397, 2813 |
| Bse118I | RCCGGY | 1 cut(s) | 1677 |
| Bse1I | ACTGG | 1 cut(s) | 2034 |
| Bse3DI | GCAATG | 1 cut(s) | 2541 |
| BseBI | CCWGG | 4 cut(s) | 220, 923, 936, 1353 |
| BseGI | GGATG | 6 cut(s) | 202, 438, 555, 756, 763, 2872 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 607, 713, 1265, 1397, 2813 |
| BseMI | GCAATG | 1 cut(s) | 2541 |
| BseMII | CTCAG | 3 cut(s) | 152, 2197, 2799 |
| BseNI | ACTGG | 1 cut(s) | 2034 |
| BseRI | GAGGAG | 2 cut(s) | 1028, 2561 |
| BseSI | GKGCMC | 2 cut(s) | 487, 1740 |
| BseX3I | CGGCCG | 1 cut(s) | 78 |
| BseXI | GCAGC | 9 cut(s) | 152, 210, 256, 259, 287, 973, 1198, 1633, 2242 |
| BsgI | GTGCAG | 1 cut(s) | 215 |
| Bsh1236I | CGCG | 3 cut(s) | 77, 444, 2385 |
| Bsh1285I | CGRYCG | 1 cut(s) | 81 |
| BshTI | ACCGGT | 1 cut(s) | 1677 |
| BsiEI | CGRYCG | 1 cut(s) | 81 |
| BsiHKAI | GWGCWC | 1 cut(s) | 888 |
| BsiHKCI | CYCGRG | 3 cut(s) | 887, 2516, 2737 |
| BsiSI | CCGG | 4 cut(s) | 101, 607, 1678, 2547 |
| BslFI | GGGAC | 4 cut(s) | 503, 2123, 2245, 2811 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 607, 713, 1265, 1397, 2813 |
| BsmAI | GTCTC | 4 cut(s) | 183, 1350, 2261, 2500 |
| BsmFI | GGGAC | 4 cut(s) | 503, 2123, 2245, 2811 |
| BsmI | GAATGC | 1 cut(s) | 2258 |
| Bso31I | GGTCTC | 1 cut(s) | 2261 |
| BsoBI | CYCGRG | 3 cut(s) | 887, 2516, 2737 |
| Bsp119I | TTCGAA | 1 cut(s) | 1515 |
| Bsp1286I | GDGCHC | 4 cut(s) | 487, 888, 979, 1740 |
| Bsp143I | GATC | 4 cut(s) | 12, 1657, 1795, 1986 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 161 |
| Bsp19I | CCATGG | 3 cut(s) | 1002, 1932, 2316 |
| Bsp68I | TCGCGA | 1 cut(s) | 2385 |
| BspACI | CCGC | 9 cut(s) | 77, 86, 396, 424, 442, 447, 819, 2031, 2252 |
| BspCNI | CTCAG | 3 cut(s) | 153, 2196, 2800 |
| BspFNI | CGCG | 3 cut(s) | 77, 444, 2385 |
| BspHI | TCATGA | 1 cut(s) | 979 |
| BspLI | GGNNCC | 1 cut(s) | 2531 |
| BspMAI | CTGCAG | 1 cut(s) | 277 |
| BspMI | ACCTGC | 1 cut(s) | 457 |
| BspPI | GGATC | 1 cut(s) | 1803 |
| BspT104I | TTCGAA | 1 cut(s) | 1515 |
| BspTNI | GGTCTC | 1 cut(s) | 2261 |
| BsrBI | CCGCTC | 2 cut(s) | 426, 449 |
| BsrDI | GCAATG | 1 cut(s) | 2541 |
| BsrFI | RCCGGY | 1 cut(s) | 1677 |
| BsrI | ACTGG | 1 cut(s) | 2034 |
| BssAI | RCCGGY | 1 cut(s) | 1677 |
| BssMI | GATC | 4 cut(s) | 12, 1657, 1795, 1986 |
| BssNI | GRCGYC | 1 cut(s) | 591 |
| BssT1I | CCWWGG | 4 cut(s) | 990, 1002, 1932, 2316 |
| Bst2UI | CCWGG | 4 cut(s) | 220, 923, 936, 1353 |
| Bst4CI | ACNGT | 6 cut(s) | 1134, 1300, 2056, 2077, 2200, 2507 |
| Bst6I | CTCTTC | 4 cut(s) | 905, 1209, 2540, 2699 |
| BstACI | GRCGYC | 1 cut(s) | 591 |
| BstBAI | YACGTR | 1 cut(s) | 529 |
| BstBI | TTCGAA | 1 cut(s) | 1515 |
| BstC8I | GCNNGC | 3 cut(s) | 309, 339, 2383 |
| BstDEI | CTNAG | 6 cut(s) | 161, 738, 2183, 2510, 2808, 2877 |
| BstDSI | CCRYGG | 5 cut(s) | 81, 825, 1002, 1932, 2316 |
| BstF5I | GGATG | 6 cut(s) | 202, 438, 555, 756, 763, 2872 |
| BstFNI | CGCG | 3 cut(s) | 77, 444, 2385 |
| BstHHI | GCGC | 2 cut(s) | 446, 2773 |
| BstKTI | GATC | 4 cut(s) | 15, 1660, 1798, 1989 |
| BstMAI | GTCTC | 4 cut(s) | 183, 1350, 2261, 2500 |
| BstMBI | GATC | 4 cut(s) | 12, 1657, 1795, 1986 |
| BstMCI | CGRYCG | 1 cut(s) | 81 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 83, 86, 229, 275, 482, 983, 992, 1162, 2552 |
| BstNI | CCWGG | 4 cut(s) | 220, 923, 936, 1353 |
| BstNSI | RCATGY | 3 cut(s) | 178, 703, 802 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 683 |
| BstSCI | CCNGG | 6 cut(s) | 218, 606, 921, 934, 1351, 2546 |
| BstSFI | CTRYAG | 1 cut(s) | 273 |
| BstSLI | GKGCMC | 2 cut(s) | 487, 1740 |
| BstSNI | TACGTA | 1 cut(s) | 529 |
| BstUI | CGCG | 3 cut(s) | 77, 444, 2385 |
| BstV1I | GCAGC | 9 cut(s) | 152, 210, 256, 259, 287, 973, 1198, 1633, 2242 |
| BstV2I | GAAGAC | 7 cut(s) | 52, 679, 933, 1106, 1504, 1878, 2350 |
| BstX2I | RGATCY | 2 cut(s) | 1657, 1795 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 997, 1380 |
| BstYI | RGATCY | 2 cut(s) | 1657, 1795 |
| BstZI | CGGCCG | 1 cut(s) | 78 |
| BsuI | GTATCC | 2 cut(s) | 1490, 1735 |
| BtgI | CCRYGG | 5 cut(s) | 81, 825, 1002, 1932, 2316 |
| BtgZI | GCGATG | 1 cut(s) | 2400 |
| BtsCI | GGATG | 6 cut(s) | 202, 438, 555, 756, 763, 2872 |
| BtsIMutI | CAGTG | 3 cut(s) | 1130, 1296, 2763 |
| BtuMI | TCGCGA | 1 cut(s) | 2385 |
| BveI | ACCTGC | 1 cut(s) | 457 |
| Cac8I | GCNNGC | 3 cut(s) | 309, 339, 2383 |
| CaiI | CAGNNNCTG | 2 cut(s) | 272, 1718 |
| CciI | TCATGA | 1 cut(s) | 979 |
| CfoI | GCGC | 2 cut(s) | 446, 2773 |
| Cfr10I | RCCGGY | 1 cut(s) | 1677 |
| Cfr13I | GGNCC | 7 cut(s) | 283, 320, 609, 627, 1883, 2187, 2530 |
| Csp6I | GTAC | 4 cut(s) | 433, 614, 717, 876 |
| CspAI | ACCGGT | 1 cut(s) | 1677 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 238, 273, 1470, 1474, 1505, 1509 |
| CviQI | GTAC | 4 cut(s) | 433, 614, 717, 876 |
| DdeI | CTNAG | 6 cut(s) | 161, 738, 2183, 2510, 2808, 2877 |
| DpnI | GATC | 4 cut(s) | 14, 1659, 1797, 1988 |
| DpnII | GATC | 4 cut(s) | 12, 1657, 1795, 1986 |
| DraIII | CACNNNGTG | 1 cut(s) | 2779 |
| DriI | GACNNNNNGTC | 1 cut(s) | 515 |
| EaeI | YGGCCR | 3 cut(s) | 78, 669, 999 |
| EagI | CGGCCG | 1 cut(s) | 78 |
| Eam1104I | CTCTTC | 4 cut(s) | 905, 1209, 2540, 2699 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 515 |
| EarI | CTCTTC | 4 cut(s) | 905, 1209, 2540, 2699 |
| EciI | GGCGGA | 1 cut(s) | 385 |
| Ecl136II | GAGCTC | 1 cut(s) | 886 |
| EclXI | CGGCCG | 1 cut(s) | 78 |
| Eco105I | TACGTA | 1 cut(s) | 529 |
| Eco130I | CCWWGG | 4 cut(s) | 990, 1002, 1932, 2316 |
| Eco24I | GRGCYC | 2 cut(s) | 888, 979 |
| Eco31I | GGTCTC | 1 cut(s) | 2261 |
| Eco47I | GGWCC | 2 cut(s) | 283, 2187 |
| Eco52I | CGGCCG | 1 cut(s) | 78 |
| Eco53kI | GAGCTC | 1 cut(s) | 886 |
| Eco57I | CTGAAG | 3 cut(s) | 1190, 2120, 2787 |
| Eco88I | CYCGRG | 3 cut(s) | 887, 2516, 2737 |
| EcoICRI | GAGCTC | 1 cut(s) | 886 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1883, 2187, 2530 |
| EcoRI | GAATTC | 2 cut(s) | 1060, 1285 |
| EcoRII | CCWGG | 4 cut(s) | 218, 921, 934, 1351 |
| EcoT14I | CCWWGG | 4 cut(s) | 990, 1002, 1932, 2316 |
| EcoT22I | ATGCAT | 2 cut(s) | 1534, 1768 |
| EcoT38I | GRGCYC | 2 cut(s) | 888, 979 |
| ErhI | CCWWGG | 4 cut(s) | 990, 1002, 1932, 2316 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1614, 1646 |
| FaqI | GGGAC | 4 cut(s) | 503, 2123, 2245, 2811 |
| FauNDI | CATATG | 1 cut(s) | 1991 |
| FbaI | TGATCA | 2 cut(s) | 12, 1986 |
| FblI | GTMKAC | 2 cut(s) | 531, 588 |
| FokI | GGATG | 6 cut(s) | 189, 425, 542, 763, 770, 2859 |
| FriOI | GRGCYC | 2 cut(s) | 888, 979 |
| FspBI | CTAG | 7 cut(s) | 66, 720, 1121, 1329, 1853, 1974, 2162 |
| GlaI | GCGC | 2 cut(s) | 445, 2772 |
| GsuI | CTGGAG | 1 cut(s) | 1374 |
| HapII | CCGG | 4 cut(s) | 101, 607, 1678, 2547 |
| HhaI | GCGC | 2 cut(s) | 446, 2773 |
| Hin1I | GRCGYC | 1 cut(s) | 591 |
| Hin6I | GCGC | 2 cut(s) | 444, 2771 |
| HinP1I | GCGC | 2 cut(s) | 444, 2771 |
| HincII | GTYRAC | 3 cut(s) | 589, 1806, 2796 |
| HindII | GTYRAC | 3 cut(s) | 589, 1806, 2796 |
| HindIII | AAGCTT | 4 cut(s) | 495, 1039, 1279, 1909 |
| HpaI | GTTAAC | 1 cut(s) | 1806 |
| HpaII | CCGG | 4 cut(s) | 101, 607, 1678, 2547 |
| HphI | GGTGA | 9 cut(s) | 115, 237, 1069, 1252, 1595, 1886, 2364, 2435, 2828 |
| Hpy166II | GTNNAC | 8 cut(s) | 532, 589, 697, 1303, 1806, 2089, 2711, 2796 |
| Hpy8I | GTNNAC | 8 cut(s) | 532, 589, 697, 1303, 1806, 2089, 2711, 2796 |
| Hpy99I | CGWCG | 5 cut(s) | 466, 593, 635, 2006, 2267 |
| HpyAV | CCTTC | 4 cut(s) | 755, 1048, 1231, 1933 |
| HpyCH4III | ACNGT | 6 cut(s) | 1134, 1300, 2056, 2077, 2200, 2507 |
| HpyCH4IV | ACGT | 5 cut(s) | 255, 528, 591, 2085, 2330 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 83, 86, 229, 275, 482, 983, 992, 1162, 2552 |
| HpyF3I | CTNAG | 6 cut(s) | 161, 738, 2183, 2510, 2808, 2877 |
| HpySE526I | ACGT | 5 cut(s) | 255, 528, 591, 2085, 2330 |
| Hsp92I | GRCGYC | 1 cut(s) | 591 |
| HspAI | GCGC | 2 cut(s) | 444, 2771 |
| Ksp22I | TGATCA | 2 cut(s) | 12, 1986 |
| KspAI | GTTAAC | 1 cut(s) | 1806 |
| Kzo9I | GATC | 4 cut(s) | 12, 1657, 1795, 1986 |
| LmnI | GCTCC | 3 cut(s) | 431, 652, 2070 |
| Lsp1109I | GCAGC | 9 cut(s) | 152, 210, 256, 259, 287, 973, 1198, 1633, 2242 |
| LweI | GCATC | 4 cut(s) | 177, 562, 973, 1633 |
| MaeI | CTAG | 7 cut(s) | 66, 720, 1121, 1329, 1853, 1974, 2162 |
| MaeII | ACGT | 5 cut(s) | 255, 528, 591, 2085, 2330 |
| MaeIII | GTNAC | 5 cut(s) | 1021, 1456, 1475, 2024, 2077 |
| MalI | GATC | 4 cut(s) | 14, 1659, 1797, 1988 |
| MbiI | CCGCTC | 2 cut(s) | 426, 449 |
| MboI | GATC | 4 cut(s) | 12, 1657, 1795, 1986 |
| MflI | RGATCY | 2 cut(s) | 1657, 1795 |
| MhlI | GDGCHC | 4 cut(s) | 487, 888, 979, 1740 |
| MlsI | TGGCCA | 2 cut(s) | 671, 1001 |
| MluNI | TGGCCA | 2 cut(s) | 671, 1001 |
| MlyI | GAGTC | 8 cut(s) | 123, 452, 1352, 1597, 2011, 2363, 2749, 2833 |
| MmeI | TCCRAC | 3 cut(s) | 1083, 2092, 2526 |
| Mox20I | TGGCCA | 2 cut(s) | 671, 1001 |
| Mph1103I | ATGCAT | 2 cut(s) | 1534, 1768 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1626, 2220 |
| MscI | TGGCCA | 2 cut(s) | 671, 1001 |
| MslI | CAYNNNNRTG | 2 cut(s) | 173, 1733 |
| Msp20I | TGGCCA | 2 cut(s) | 671, 1001 |
| MspA1I | CMGCKG | 1 cut(s) | 269 |
| MspI | CCGG | 4 cut(s) | 101, 607, 1678, 2547 |
| MspR9I | CCNGG | 6 cut(s) | 220, 608, 923, 936, 1353, 2548 |
| Mva1269I | GAATGC | 1 cut(s) | 2258 |
| MvaI | CCWGG | 4 cut(s) | 220, 923, 936, 1353 |
| MvnI | CGCG | 3 cut(s) | 77, 444, 2385 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 83, 86, 229, 275, 482, 983, 992, 1162, 2552 |
| NciI | CCSGG | 2 cut(s) | 608, 2548 |
| NcoI | CCATGG | 3 cut(s) | 1002, 1932, 2316 |
| NdeI | CATATG | 1 cut(s) | 1991 |
| NdeII | GATC | 4 cut(s) | 12, 1657, 1795, 1986 |
| NlaIV | GGNNCC | 1 cut(s) | 2531 |
| NmuCI | GTSAC | 2 cut(s) | 1021, 1456 |
| NruI | TCGCGA | 1 cut(s) | 2385 |
| NsiI | ATGCAT | 2 cut(s) | 1534, 1768 |
| NspI | RCATGY | 3 cut(s) | 178, 703, 802 |
| NspV | TTCGAA | 1 cut(s) | 1515 |
| PaeR7I | CTCGAG | 3 cut(s) | 887, 2516, 2737 |
| PagI | TCATGA | 1 cut(s) | 979 |
| PciI | ACATGT | 3 cut(s) | 174, 699, 798 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 534, 543, 2744 |
| PctI | GAATGC | 1 cut(s) | 2258 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1626, 2220 |
| PfeI | GAWTC | 7 cut(s) | 326, 597, 1387, 1466, 1715, 2621, 2627 |
| PfoI | TCCNGGA | 1 cut(s) | 921 |
| PinAI | ACCGGT | 1 cut(s) | 1677 |
| PleI | GAGTC | 8 cut(s) | 122, 452, 1352, 1596, 2011, 2363, 2748, 2832 |
| PpsI | GAGTC | 8 cut(s) | 122, 452, 1352, 1596, 2011, 2363, 2748, 2832 |
| Ppu21I | YACGTR | 1 cut(s) | 529 |
| PpuMI | RGGWCCY | 1 cut(s) | 2187 |
| PscI | ACATGT | 3 cut(s) | 174, 699, 798 |
| PshAI | GACNNNNGTC | 1 cut(s) | 683 |
| PsiI | TTATAA | 1 cut(s) | 353 |
| Psp124BI | GAGCTC | 1 cut(s) | 888 |
| Psp1406I | AACGTT | 1 cut(s) | 2330 |
| Psp5II | RGGWCCY | 1 cut(s) | 2187 |
| Psp6I | CCWGG | 4 cut(s) | 218, 921, 934, 1351 |
| PspGI | CCWGG | 4 cut(s) | 218, 921, 934, 1351 |
| PspN4I | GGNNCC | 1 cut(s) | 2531 |
| PspPI | GGNCC | 7 cut(s) | 283, 320, 609, 627, 1883, 2187, 2530 |
| PspPPI | RGGWCCY | 1 cut(s) | 2187 |
| PspXI | VCTCGAGB | 2 cut(s) | 887, 2737 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1295, 1327 |
| PstI | CTGCAG | 1 cut(s) | 277 |
| PstNI | CAGNNNCTG | 2 cut(s) | 272, 1718 |
| PsuI | RGATCY | 2 cut(s) | 1657, 1795 |
| PvuII | CAGCTG | 1 cut(s) | 269 |
| RruI | TCGCGA | 1 cut(s) | 2385 |
| RsaI | GTAC | 4 cut(s) | 434, 615, 718, 877 |
| RsaNI | GTAC | 4 cut(s) | 433, 614, 717, 876 |
| RseI | CAYNNNNRTG | 2 cut(s) | 173, 1733 |
| SacI | GAGCTC | 1 cut(s) | 888 |
| SalI | GTCGAC | 1 cut(s) | 587 |
| Sau3AI | GATC | 4 cut(s) | 12, 1657, 1795, 1986 |
| Sau96I | GGNCC | 7 cut(s) | 283, 320, 609, 627, 1883, 2187, 2530 |
| SchI | GAGTC | 8 cut(s) | 123, 452, 1352, 1597, 2011, 2363, 2749, 2833 |
| ScrFI | CCNGG | 6 cut(s) | 220, 608, 923, 936, 1353, 2548 |
| SduI | GDGCHC | 4 cut(s) | 487, 888, 979, 1740 |
| SfaNI | GCATC | 4 cut(s) | 177, 562, 973, 1633 |
| SfcI | CTRYAG | 1 cut(s) | 273 |
| Sfr274I | CTCGAG | 3 cut(s) | 887, 2516, 2737 |
| SfuI | TTCGAA | 1 cut(s) | 1515 |
| SinI | GGWCC | 2 cut(s) | 283, 2187 |
| SlaI | CTCGAG | 3 cut(s) | 887, 2516, 2737 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 173, 1733 |
| SmlI | CTYRAG | 3 cut(s) | 887, 2516, 2737 |
| SmoI | CTYRAG | 3 cut(s) | 887, 2516, 2737 |
| SnaBI | TACGTA | 1 cut(s) | 529 |
| SpeI | ACTAGT | 2 cut(s) | 1120, 1328 |
| SsiI | CCGC | 9 cut(s) | 77, 86, 396, 424, 442, 447, 819, 2031, 2252 |
| SspI | AATATT | 1 cut(s) | 22 |
| SspMI | CTAG | 7 cut(s) | 66, 720, 1121, 1329, 1853, 1974, 2162 |
| SstI | GAGCTC | 1 cut(s) | 888 |
| StyD4I | CCNGG | 6 cut(s) | 218, 606, 921, 934, 1351, 2546 |
| StyI | CCWWGG | 4 cut(s) | 990, 1002, 1932, 2316 |
| TaaI | ACNGT | 6 cut(s) | 1134, 1300, 2056, 2077, 2200, 2507 |
| TaiI | ACGT | 5 cut(s) | 258, 531, 594, 2088, 2333 |
| TatI | WGTACW | 1 cut(s) | 875 |
| TauI | GCSGC | 4 cut(s) | 80, 89, 449, 2255 |
| TfiI | GAWTC | 7 cut(s) | 326, 597, 1387, 1466, 1715, 2621, 2627 |
| TscAI | CASTG | 3 cut(s) | 1137, 1303, 2770 |
| TseFI | GTSAC | 2 cut(s) | 1021, 1456 |
| TseI | GCWGC | 9 cut(s) | 165, 223, 269, 272, 275, 986, 1186, 1646, 2255 |
| Tsp45I | GTSAC | 2 cut(s) | 1021, 1456 |
| TspGWI | ACGGA | 1 cut(s) | 814 |
| TspRI | CASTG | 3 cut(s) | 1137, 1303, 2770 |
| VpaK11BI | GGWCC | 2 cut(s) | 283, 2187 |
| XapI | RAATTY | 4 cut(s) | 1060, 1144, 1285, 2664 |
| XbaI | TCTAGA | 1 cut(s) | 1852 |
| XceI | RCATGY | 3 cut(s) | 178, 703, 802 |
| XhoI | CTCGAG | 3 cut(s) | 887, 2516, 2737 |
| XmiI | GTMKAC | 2 cut(s) | 531, 588 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1626, 2220 |
| XspI | CTAG | 7 cut(s) | 66, 720, 1121, 1329, 1853, 1974, 2162 |
| ZraI | GACGTC | 1 cut(s) | 592 |
| Zsp2I | ATGCAT | 2 cut(s) | 1534, 1768 |