MD15G1095100.v1.1

Bark storage protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000829
Physical Location & Seq
Reverse (-)
6645950 .. 6647272
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1095100.v1.1.491

Sequence Viewer

Length: 459 bp
ATGAGAACTTTATCAGTAGCTTCCATACTTCTCTATGCTCTCTTGATGCTTAGTCCACTGCAGTTAAATGTTGCATTGTCAACTGAGACACAAATGTTGATTGAGGAGGCCAACATGAGTGGCCCTTATTTAGGACTTGTCATTCCAAATTCTTATGAAATGGATCCTCTTCTGCAATCTCCAAACTTCACTTCAAGTAACCTGTTCATAGATTTCTCTGGGAGGAGATTGCGATTCGGAACAATTGCTAATAAGCCAGTCATATTGGTCATGACAGGACTGTCAATGATTAATGCAGCAATAACTACTCAGCTATTACTGAGCCAGTTTGATATAGAAGGAGTGGTGCACTATGGCATAGCAGGGCATGCAAACCCATCCCTCAATCTTGCACATGTGGTCATTCCTCAGTATTGGTCCCATTCTGCTCTTTGGAGCTGGCAGGTTGGTGCCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNP_UDP_1

Protein Analysis

153

Amino Acids

16.65

Weight (kDa)

5.67

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNP_UDP_1 PF01048 44 - 138 2.1e-14 Phosphorylase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 280
Acc36I ACCTGC 1 cut(s) 433
AccB1I GGYRCC 1 cut(s) 449
AclWI GGATC 2 cut(s) 158, 171
AcsI RAATTY 1 cut(s) 148
AfiI CCNNNNNNNGG 1 cut(s) 131
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 1 cut(s) 195
AjuI GAANNNNNNNTTGG 2 cut(s) 175, 207
AluBI AGCT 4 cut(s) 20, 313, 438, 456
AluI AGCT 4 cut(s) 20, 313, 438, 456
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 1 cut(s) 80
Alw44I GTGCAC 1 cut(s) 347
AlwI GGATC 2 cut(s) 158, 171
AoxI GGCC 2 cut(s) 108, 121
ApaLI GTGCAC 1 cut(s) 347
ApeKI GCWGC 1 cut(s) 296
ApoI RAATTY 1 cut(s) 148
AseI ATTAAT 1 cut(s) 291
AspS9I GGNCC 2 cut(s) 122, 417
AvaII GGWCC 1 cut(s) 417
BaeGI GKGCMC 1 cut(s) 351
BamHI GGATCC 1 cut(s) 163
BanI GGYRCC 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 351
BbvI GCAGC 1 cut(s) 308
BccI CCATC 1 cut(s) 385
BcoDI GTCTC 1 cut(s) 80
BfmI CTRYAG 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 433
BisI GCNGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 298
Bme18I GGWCC 1 cut(s) 417
BmgT120I GGNCC 2 cut(s) 122, 417
BmiI GGNNCC 3 cut(s) 165, 419, 451
BmsI GCATC 1 cut(s) 36
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse1I ACTGG 2 cut(s) 257, 325
BseGI GGATG 1 cut(s) 377
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMII CTCAG 4 cut(s) 75, 311, 323, 422
BseNI ACTGG 2 cut(s) 257, 325
BseRI GAGGAG 2 cut(s) 119, 238
BseSI GKGCMC 1 cut(s) 351
BseXI GCAGC 1 cut(s) 308
BshFI GGCC 2 cut(s) 110, 123
BshNI GGYRCC 1 cut(s) 449
BsiHKAI GWGCWC 1 cut(s) 351
BslFI GGGAC 1 cut(s) 403
BslI CCNNNNNNNGG 1 cut(s) 131
BsmAI GTCTC 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 403
BsnI GGCC 2 cut(s) 110, 123
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 1 cut(s) 163
BspANI GGCC 2 cut(s) 110, 123
BspCNI CTCAG 4 cut(s) 76, 312, 322, 421
BspHI TCATGA 1 cut(s) 270
BspLI GGNNCC 3 cut(s) 165, 419, 451
BspMAI CTGCAG 1 cut(s) 63
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 2 cut(s) 158, 171
BspT107I GGYRCC 1 cut(s) 449
BsrI ACTGG 2 cut(s) 257, 325
BssMI GATC 1 cut(s) 163
Bst4CI ACNGT 1 cut(s) 282
Bst6I CTCTTC 1 cut(s) 174
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 3 cut(s) 369, 440, 454
BstDEI CTNAG 5 cut(s) 50, 84, 309, 320, 408
BstENI CCTNNNNNAGG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 377
BstKTI GATC 1 cut(s) 166
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 1 cut(s) 163
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNSI RCATGY 2 cut(s) 371, 398
BstSFI CTRYAG 1 cut(s) 59
BstSLI GKGCMC 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
BsuRI GGCC 2 cut(s) 110, 123
BtsCI GGATG 1 cut(s) 377
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 1 cut(s) 56
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 3 cut(s) 369, 440, 454
CciI TCATGA 1 cut(s) 270
Cfr13I GGNCC 2 cut(s) 122, 417
CspCI CAANNNNNGTGG 2 cut(s) 100, 135
CviAII CATG 4 cut(s) 115, 271, 368, 395
CviJI RGCY 8 cut(s) 20, 110, 123, 256, 313, 324, 438, 456
CviKI_1 RGCY 8 cut(s) 20, 110, 123, 256, 313, 324, 438, 456
DdeI CTNAG 5 cut(s) 50, 84, 309, 320, 408
DpnI GATC 1 cut(s) 165
DpnII GATC 1 cut(s) 163
DrdI GACNNNNNNGTC 1 cut(s) 280
DseDI GACNNNNNNGTC 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 417
EcoNI CCTNNNNNAGG 1 cut(s) 129
FaeI CATG 4 cut(s) 118, 274, 371, 398
FaqI GGGAC 1 cut(s) 403
FatI CATG 4 cut(s) 114, 270, 367, 394
Fnu4HI GCNGC 1 cut(s) 297
FokI GGATG 1 cut(s) 364
Fsp4HI GCNGC 1 cut(s) 297
GluI GCNGC 1 cut(s) 297
HaeIII GGCC 2 cut(s) 110, 123
Hin1II CATG 4 cut(s) 118, 274, 371, 398
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 1 cut(s) 234
Hpy166II GTNNAC 3 cut(s) 56, 81, 349
Hpy188I TCNGA 1 cut(s) 239
Hpy188III TCNNGA 2 cut(s) 43, 271
Hpy8I GTNNAC 3 cut(s) 56, 81, 349
HpyAV CCTTC 1 cut(s) 332
HpyCH4III ACNGT 1 cut(s) 282
HpyCH4V TGCA 7 cut(s) 61, 74, 175, 296, 349, 371, 392
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpyF3I CTNAG 5 cut(s) 50, 84, 309, 320, 408
Hsp92II CATG 4 cut(s) 118, 274, 371, 398
Kzo9I GATC 1 cut(s) 163
LmnI GCTCC 1 cut(s) 435
LpnPI CCDG 8 cut(s) 204, 215, 261, 270, 338, 348, 424, 428
Lsp1109I GCAGC 1 cut(s) 308
LweI GCATC 1 cut(s) 36
MaeIII GTNAC 1 cut(s) 197
MalI GATC 1 cut(s) 165
MboI GATC 1 cut(s) 163
MboII GAAGA 1 cut(s) 161
MfeI CAATTG 1 cut(s) 243
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 1 cut(s) 351
MluCI AATT 2 cut(s) 148, 243
MnlI CCTC 6 cut(s) 97, 100, 177, 216, 392, 417
MseI TTAA 2 cut(s) 65, 291
MunI CAATTG 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 368
NdeII GATC 1 cut(s) 163
NlaIII CATG 4 cut(s) 118, 274, 371, 398
NlaIV GGNNCC 3 cut(s) 165, 419, 451
NspI RCATGY 2 cut(s) 371, 398
PaeI GCATGC 1 cut(s) 371
PagI TCATGA 1 cut(s) 270
PciI ACATGT 1 cut(s) 394
PfeI GAWTC 1 cut(s) 234
PkrI GCNGC 1 cut(s) 298
PscI ACATGT 1 cut(s) 394
PshBI ATTAAT 1 cut(s) 291
PspN4I GGNNCC 3 cut(s) 165, 419, 451
PspPI GGNCC 2 cut(s) 122, 417
PstI CTGCAG 1 cut(s) 63
PsuI RGATCY 1 cut(s) 163
SaqAI TTAA 2 cut(s) 65, 291
SatI GCNGC 1 cut(s) 297
Sau3AI GATC 1 cut(s) 163
Sau96I GGNCC 2 cut(s) 122, 417
SduI GDGCHC 1 cut(s) 351
SetI ASST 6 cut(s) 22, 204, 315, 440, 447, 458
SfaNI GCATC 1 cut(s) 36
SfcI CTRYAG 1 cut(s) 59
SinI GGWCC 1 cut(s) 417
SphI GCATGC 1 cut(s) 371
Sse9I AATT 2 cut(s) 148, 243
TaaI ACNGT 1 cut(s) 282
TasI AATT 2 cut(s) 148, 243
TfiI GAWTC 1 cut(s) 234
Tru1I TTAA 2 cut(s) 65, 291
Tru9I TTAA 2 cut(s) 65, 291
TscAI CASTG 1 cut(s) 63
TseI GCWGC 1 cut(s) 296
TspDTI ATGAA 2 cut(s) 171, 196
TspRI CASTG 1 cut(s) 63
VneI GTGCAC 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 417
VspI ATTAAT 1 cut(s) 291
XagI CCTNNNNNAGG 1 cut(s) 129
XapI RAATTY 1 cut(s) 148
XceI RCATGY 2 cut(s) 371, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.