MD15G1191300.v1.1

Bark storage protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000829
Physical Location & Seq
Reverse (-)
15040574 .. 15043088
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1191300.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGAGGGAAGGAGGGAGATGGAGAGCCGTTTGCGGATGGAGTTGATGGTTTTTCTTGTGTTGGTTTTGGGGTTTAGCAGTAGATCCTTTGGTGCAGTTTCTCACAGTACCATGAACAAGATTTATAGAATAAACAAAGAAGGTCCATATTTGGGCATTGTAGTACCAAATTTCTTTGAGATGAACCCTCTTCTTCAATCTACAAGCTTTGTGGCTGATCAGAAGCTACCGTATTTGGATTTTGCTGGAAGAAGGTTTCGAATCGGACGATTGGAAGACAAAAAGGTTATAATTGTTATGACTGGATTGAGCATGCTCAATGCAGGCATCTCAACTCAATTACTACAAACCCTATTCATGGTGAAAGGTGTTGTACACTATGGAATTGCTGGAAATGCAGATCCCCAACTCCAAATTGGAGATGTCACCATCCCTCAATTTTGGGCTCATACAGGCCTTTGGAATTGGCAGAGGTTTGGAGATGGGCCTAATGATGAATTGTCCTTAGAATCATTTGGAGACTACACAAGAGAAGTTGGTTATATAAAATTTTCAGATTTTAGTAACAAAACCAAAGTTGGAACTTCTGTTCCCAATCTCCTGAACAGTGCATGGTATCAGCCAGAAGAGACCTTCCCAGTCCATGGCACTCCGGAAGTTAGGCAACATGCCTTTTGGGTTCCAGTCAACCACAAATATTTTTCACTTACCAAGAAACTAGAGGGTTTGAAGTTGGGGAGCTGTGTCAATACAACTTGTCTTCCAAGAACACCCAAAGTGGTGGCAGTCCAAAGGGGGATTAGTGCAAATGTGTTTGTTGACAATAGTGCATACAGAGAATTCTTGAATTCCAAATTCAATGCCACCCCAATAGACATGGAAAGTGCTGCAGTGGCTCTTGTATGTCACCAGCAGAACACACCGTTTATCGTAATTAGAGCTCTCTCTGATTTGGCTGGTGGAGGATCCTCTCTCTCCAATGAAGCCAACACCTTTGCGTCATTGGCAGCTCAAAATGCAGTTGATGTTGTACTCAGATTCGTTGCCTTGTTGTCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNP_UDP_1

Protein Analysis

354

Amino Acids

39.23

Weight (kDa)

8.67

Isoelectric Point (pI)

28.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNP_UDP_1 PF01048 51 - 349 6.8e-26 Phosphorylase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 290
AccB7I CCANNNNNTGG 1 cut(s) 644
AccIII TCCGGA 1 cut(s) 652
AciI CCGC 1 cut(s) 34
AclWI GGATC 4 cut(s) 78, 395, 960, 973
AcsI RAATTY 5 cut(s) 169, 548, 839, 847, 854
AfaI GTAC 4 cut(s) 109, 165, 375, 1032
AfiI CCNNNNNNNGG 3 cut(s) 152, 358, 644
AgsI TTSAA 4 cut(s) 197, 730, 847, 859
AloI GAACNNNNNNTCC 4 cut(s) 573, 574, 605, 606
AluBI AGCT 5 cut(s) 207, 226, 741, 941, 1010
AluI AGCT 5 cut(s) 207, 226, 741, 941, 1010
Alw21I GWGCWC 1 cut(s) 943
Alw26I GTCTC 2 cut(s) 513, 623
AlwI GGATC 4 cut(s) 78, 395, 960, 973
Aor13HI TCCGGA 1 cut(s) 652
AoxI GGCC 2 cut(s) 454, 485
ApeKI GCWGC 2 cut(s) 887, 1007
ApoI RAATTY 5 cut(s) 169, 548, 839, 847, 854
AspS9I GGNCC 2 cut(s) 143, 485
AsuHPI GGTGA 3 cut(s) 373, 418, 899
AsuII TTCGAA 1 cut(s) 259
AvaII GGWCC 1 cut(s) 143
BamHI GGATCC 1 cut(s) 965
BanII GRGCYC 2 cut(s) 448, 943
BbsI GAAGAC 3 cut(s) 282, 752, 1047
Bbv12I GWGCWC 1 cut(s) 943
BbvI GCAGC 2 cut(s) 874, 1019
BccI CCATC 5 cut(s) 13, 31, 40, 437, 476
BceAI ACGGC 1 cut(s) 12
BclI TGATCA 1 cut(s) 217
BcoDI GTCTC 2 cut(s) 513, 623
BfaI CTAG 1 cut(s) 719
BfmI CTRYAG 1 cut(s) 888
BisI GCNGC 2 cut(s) 888, 1008
BlsI GCNGC 2 cut(s) 889, 1009
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 2 cut(s) 143, 485
BmiI GGNNCC 2 cut(s) 681, 967
BmrI ACTGGG 1 cut(s) 632
BmsI GCATC 1 cut(s) 336
BmuI ACTGGG 1 cut(s) 632
BpiI GAAGAC 3 cut(s) 282, 752, 1047
Bpu14I TTCGAA 1 cut(s) 259
BsaI GGTCTC 1 cut(s) 623
BsaJI CCNNGG 1 cut(s) 643
BsaWI WCCGGW 1 cut(s) 652
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 358, 644
Bse1I ACTGG 3 cut(s) 307, 638, 683
BseAI TCCGGA 1 cut(s) 652
BseDI CCNNGG 1 cut(s) 643
BseGI GGATG 2 cut(s) 42, 429
BseLI CCNNNNNNNGG 3 cut(s) 152, 358, 644
BseMII CTCAG 1 cut(s) 1048
BseNI ACTGG 3 cut(s) 307, 638, 683
BseXI GCAGC 2 cut(s) 874, 1019
BsgI GTGCAG 1 cut(s) 114
BshFI GGCC 2 cut(s) 456, 487
BsiHKAI GWGCWC 1 cut(s) 943
BsiSI CCGG 1 cut(s) 653
BslI CCNNNNNNNGG 3 cut(s) 152, 358, 644
BsmAI GTCTC 2 cut(s) 513, 623
BsnI GGCC 2 cut(s) 456, 487
Bso31I GGTCTC 1 cut(s) 623
Bsp119I TTCGAA 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 448, 943
Bsp13I TCCGGA 1 cut(s) 652
Bsp1407I TGTACA 1 cut(s) 373
Bsp143I GATC 4 cut(s) 83, 217, 400, 965
Bsp19I CCATGG 1 cut(s) 643
BspACI CCGC 1 cut(s) 34
BspANI GGCC 2 cut(s) 456, 487
BspCNI CTCAG 1 cut(s) 1047
BspEI TCCGGA 1 cut(s) 652
BspLI GGNNCC 2 cut(s) 681, 967
BspMAI CTGCAG 1 cut(s) 892
BspPI GGATC 4 cut(s) 78, 395, 960, 973
BspT104I TTCGAA 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 623
BsrGI TGTACA 1 cut(s) 373
BsrI ACTGG 3 cut(s) 307, 638, 683
BssECI CCNNGG 1 cut(s) 643
BssMI GATC 4 cut(s) 83, 217, 400, 965
BssT1I CCWWGG 1 cut(s) 643
Bst4CI ACNGT 4 cut(s) 107, 231, 608, 924
Bst6I CTCTTC 2 cut(s) 195, 621
BstAUI TGTACA 1 cut(s) 373
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 2 cut(s) 314, 325
BstDEI CTNAG 2 cut(s) 505, 1034
BstDSI CCRYGG 1 cut(s) 643
BstF5I GGATG 2 cut(s) 42, 429
BstKTI GATC 4 cut(s) 86, 220, 403, 968
BstMAI GTCTC 2 cut(s) 513, 623
BstMBI GATC 4 cut(s) 83, 217, 400, 965
BstMWI GCNNNNNNNGC 4 cut(s) 395, 893, 1004, 1016
BstNSI RCATGY 2 cut(s) 316, 671
BstSFI CTRYAG 1 cut(s) 888
BstV1I GCAGC 2 cut(s) 874, 1019
BstV2I GAAGAC 3 cut(s) 282, 752, 1047
BstX2I RGATCY 3 cut(s) 83, 400, 965
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 3 cut(s) 83, 400, 965
BsuRI GGCC 2 cut(s) 456, 487
BtgI CCRYGG 1 cut(s) 643
BtsCI GGATG 2 cut(s) 42, 429
BtsI GCAGTG 1 cut(s) 897
BtsIMutI CAGTG 2 cut(s) 613, 897
Cac8I GCNNGC 2 cut(s) 314, 325
Cfr13I GGNCC 2 cut(s) 143, 485
CseI GACGC 1 cut(s) 987
Csp6I GTAC 4 cut(s) 108, 164, 374, 1031
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviAII CATG 7 cut(s) 112, 313, 358, 612, 644, 668, 877
CviQI GTAC 4 cut(s) 108, 164, 374, 1031
DdeI CTNAG 2 cut(s) 505, 1034
DpnI GATC 4 cut(s) 85, 219, 402, 967
DpnII GATC 4 cut(s) 83, 217, 400, 965
Eam1104I CTCTTC 2 cut(s) 195, 621
EarI CTCTTC 2 cut(s) 195, 621
Ecl136II GAGCTC 1 cut(s) 941
Eco130I CCWWGG 1 cut(s) 643
Eco147I AGGCCT 1 cut(s) 456
Eco24I GRGCYC 2 cut(s) 448, 943
Eco31I GGTCTC 1 cut(s) 623
Eco47I GGWCC 1 cut(s) 143
Eco53kI GAGCTC 1 cut(s) 941
EcoICRI GAGCTC 1 cut(s) 941
EcoRI GAATTC 2 cut(s) 839, 847
EcoT14I CCWWGG 1 cut(s) 643
EcoT38I GRGCYC 2 cut(s) 448, 943
ErhI CCWWGG 1 cut(s) 643
FaeI CATG 7 cut(s) 115, 316, 361, 615, 647, 671, 880
FatI CATG 7 cut(s) 111, 312, 357, 611, 643, 667, 876
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 2 cut(s) 888, 1008
FokI GGATG 2 cut(s) 49, 416
FriOI GRGCYC 2 cut(s) 448, 943
Fsp4HI GCNGC 2 cut(s) 888, 1008
FspBI CTAG 1 cut(s) 719
GluI GCNGC 2 cut(s) 888, 1008
HaeIII GGCC 2 cut(s) 456, 487
HapII CCGG 1 cut(s) 653
HgaI GACGC 1 cut(s) 987
Hin1II CATG 7 cut(s) 115, 316, 361, 615, 647, 671, 880
HincII GTYRAC 2 cut(s) 688, 820
HindII GTYRAC 2 cut(s) 688, 820
HindIII AAGCTT 1 cut(s) 205
HinfI GANTC 3 cut(s) 261, 509, 1038
HpaII CCGG 1 cut(s) 653
HphI GGTGA 3 cut(s) 373, 418, 899
Hpy166II GTNNAC 3 cut(s) 376, 688, 820
Hpy188I TCNGA 5 cut(s) 222, 266, 556, 949, 1037
Hpy188III TCNNGA 3 cut(s) 601, 653, 844
Hpy8I GTNNAC 3 cut(s) 376, 688, 820
HpyAV CCTTC 4 cut(s) 3, 134, 246, 643
HpyCH4III ACNGT 4 cut(s) 107, 231, 608, 924
HpyCH4V TGCA 8 cut(s) 95, 323, 398, 611, 806, 830, 890, 1019
HpyF10VI GCNNNNNNNGC 4 cut(s) 395, 893, 1004, 1016
HpyF3I CTNAG 2 cut(s) 505, 1034
Hsp92II CATG 7 cut(s) 115, 316, 361, 615, 647, 671, 880
Kpn2I TCCGGA 1 cut(s) 652
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 4 cut(s) 83, 217, 400, 965
LmnI GCTCC 1 cut(s) 738
Lsp1109I GCAGC 2 cut(s) 874, 1019
LweI GCATC 1 cut(s) 336
MaeI CTAG 1 cut(s) 719
MaeIII GTNAC 3 cut(s) 424, 563, 905
MalI GATC 4 cut(s) 85, 219, 402, 967
MboI GATC 4 cut(s) 83, 217, 400, 965
MboII GAAGA 7 cut(s) 182, 185, 261, 287, 638, 752, 1047
MflI RGATCY 3 cut(s) 83, 400, 965
MhlI GDGCHC 2 cut(s) 448, 943
MmeI TCCRAC 1 cut(s) 559
MnlI CCTC 7 cut(s) 6, 198, 444, 465, 715, 956, 979
MroI TCCGGA 1 cut(s) 652
MspI CCGG 1 cut(s) 653
MwoI GCNNNNNNNGC 4 cut(s) 395, 893, 1004, 1016
NcoI CCATGG 1 cut(s) 643
NdeII GATC 4 cut(s) 83, 217, 400, 965
NlaIII CATG 7 cut(s) 115, 316, 361, 615, 647, 671, 880
NlaIV GGNNCC 2 cut(s) 681, 967
NmuCI GTSAC 2 cut(s) 424, 905
NspI RCATGY 2 cut(s) 316, 671
NspV TTCGAA 1 cut(s) 259
PaeI GCATGC 1 cut(s) 316
PceI AGGCCT 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 265
PfeI GAWTC 3 cut(s) 261, 509, 1038
PflMI CCANNNNNTGG 1 cut(s) 644
PkrI GCNGC 2 cut(s) 889, 1009
PsiI TTATAA 1 cut(s) 290
Psp124BI GAGCTC 1 cut(s) 943
PspN4I GGNNCC 2 cut(s) 681, 967
PspPI GGNCC 2 cut(s) 143, 485
PstI CTGCAG 1 cut(s) 892
PsuI RGATCY 3 cut(s) 83, 400, 965
RsaI GTAC 4 cut(s) 109, 165, 375, 1032
RsaNI GTAC 4 cut(s) 108, 164, 374, 1031
SacI GAGCTC 1 cut(s) 943
SatI GCNGC 2 cut(s) 888, 1008
Sau3AI GATC 4 cut(s) 83, 217, 400, 965
Sau96I GGNCC 2 cut(s) 143, 485
SduI GDGCHC 2 cut(s) 448, 943
SfaNI GCATC 1 cut(s) 336
SfcI CTRYAG 1 cut(s) 888
SfuI TTCGAA 1 cut(s) 259
SinI GGWCC 1 cut(s) 143
SphI GCATGC 1 cut(s) 316
SseBI AGGCCT 1 cut(s) 456
SsiI CCGC 1 cut(s) 34
SspI AATATT 1 cut(s) 698
SspMI CTAG 1 cut(s) 719
SstI GAGCTC 1 cut(s) 943
StuI AGGCCT 1 cut(s) 456
StyI CCWWGG 1 cut(s) 643
TaaI ACNGT 4 cut(s) 107, 231, 608, 924
TaqI TCGA 1 cut(s) 259
TatI WGTACW 2 cut(s) 373, 1030
TfiI GAWTC 3 cut(s) 261, 509, 1038
TscAI CASTG 2 cut(s) 613, 897
TseFI GTSAC 2 cut(s) 424, 905
TseI GCWGC 2 cut(s) 887, 1007
Tsp45I GTSAC 2 cut(s) 424, 905
TspDTI ATGAA 6 cut(s) 128, 197, 346, 510, 996, 1047
TspRI CASTG 2 cut(s) 613, 897
Van91I CCANNNNNTGG 1 cut(s) 644
VpaK11BI GGWCC 1 cut(s) 143
XapI RAATTY 5 cut(s) 169, 548, 839, 847, 854
XceI RCATGY 2 cut(s) 316, 671
XcmI CCANNNNNNNNNTGG 1 cut(s) 413
XspI CTAG 1 cut(s) 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.