MD15G1095300.v1.1

Bark storage protein A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000829
Physical Location & Seq
Reverse (-)
6649283 .. 6650632
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1095300.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGGAGCTTCATCAGTAGTAGCTTCTCTGATGATGATCAGCTTCTTCATGCTTAACAGACAACTGCAGGCAGATGCTGCATTGTCCGCAGAGACATACAAGTCGATCGACGAGGCGAACCAGAGTGGCCCTTATCTAGGCGTGGTCATTCCAAACGCTTTCGAAATGAACCCTCTTCTTCAATCTCCAAACTTCACCTCAACTAACCTTACCATAGATGTCTCCGGAAGAAGATTTCGGTTCGGAACCATTGCTAACAAGAAAGTCATATTGGTTATGACAGGACTCTCAATGATTAATGCAGGAATTACTACTCAGCTTTTGTTAAGCCTCTTCGACATAGAAGGAGTGGTGCATTATGGCATAGCTGGTAATGCAAACCCATCCATAAACATTGCCGACGTGGTCATTCCTCAGTATTGGGCCCATAGTGCTCTTTGGAACTGGCAGGTGCCTCCTCTTCCTAATTACGGCCACAAGTCGCTTTTACCAACTAACTGGAAATTCACACAACCTTATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNP_UDP_1

Protein Analysis

175

Amino Acids

19.17

Weight (kDa)

6.82

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNP_UDP_1 PF01048 46 - 141 3.6e-15 Phosphorylase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 439
AasI GACNNNNNNGTC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 439
AccB1I GGYRCC 1 cut(s) 451
AccIII TCCGGA 1 cut(s) 224
AciI CCGC 1 cut(s) 87
AcoI YGGCCR 1 cut(s) 472
AcsI RAATTY 1 cut(s) 503
AfiI CCNNNNNNNGG 2 cut(s) 137, 470
AgsI TTSAA 1 cut(s) 182
AjiI CACGTC 1 cut(s) 403
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
AluBI AGCT 5 cut(s) 8, 23, 42, 319, 368
AluI AGCT 5 cut(s) 8, 23, 42, 319, 368
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 2 cut(s) 86, 226
AlwNI CAGNNNCTG 1 cut(s) 77
Aor13HI TCCGGA 1 cut(s) 224
AoxI GGCC 3 cut(s) 127, 423, 472
ApaI GGGCCC 1 cut(s) 427
ApeKI GCWGC 1 cut(s) 77
ApoI RAATTY 1 cut(s) 503
AseI ATTAAT 1 cut(s) 297
AspS9I GGNCC 3 cut(s) 128, 423, 424
AsuHPI GGTGA 1 cut(s) 187
AsuII TTCGAA 1 cut(s) 162
BaeGI GKGCMC 1 cut(s) 427
BanI GGYRCC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 436
BbvI GCAGC 1 cut(s) 64
BccI CCATC 1 cut(s) 391
BceAI ACGGC 1 cut(s) 487
BclI TGATCA 1 cut(s) 36
BcoDI GTCTC 2 cut(s) 86, 226
BfaI CTAG 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 65
BfuAI ACCTGC 1 cut(s) 439
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
BmgBI CACGTC 1 cut(s) 403
BmgT120I GGNCC 3 cut(s) 128, 423, 424
BmiI GGNNCC 3 cut(s) 247, 425, 453
BmsI GCATC 1 cut(s) 64
Bpu14I TTCGAA 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 35
BsaWI WCCGGW 1 cut(s) 224
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 470
Bse1I ACTGG 2 cut(s) 449, 503
Bse3DI GCAATG 2 cut(s) 249, 393
Bse8I GATNNNNATC 1 cut(s) 35
BseAI TCCGGA 1 cut(s) 224
BseGI GGATG 1 cut(s) 383
BseJI GATNNNNATC 1 cut(s) 35
BseLI CCNNNNNNNGG 2 cut(s) 137, 470
BseMI GCAATG 2 cut(s) 249, 393
BseMII CTCAG 2 cut(s) 329, 428
BseNI ACTGG 2 cut(s) 449, 503
BseRI GAGGAG 1 cut(s) 447
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 3 cut(s) 129, 425, 474
BshNI GGYRCC 1 cut(s) 451
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 1 cut(s) 225
BslI CCNNNNNNNGG 2 cut(s) 137, 470
BsmAI GTCTC 2 cut(s) 86, 226
BsnI GGCC 3 cut(s) 129, 425, 474
Bsp119I TTCGAA 1 cut(s) 162
Bsp120I GGGCCC 1 cut(s) 423
Bsp1286I GDGCHC 2 cut(s) 427, 436
Bsp13I TCCGGA 1 cut(s) 224
Bsp143I GATC 2 cut(s) 36, 105
BspACI CCGC 1 cut(s) 87
BspANI GGCC 3 cut(s) 129, 425, 474
BspCNI CTCAG 2 cut(s) 328, 427
BspEI TCCGGA 1 cut(s) 224
BspLI GGNNCC 3 cut(s) 247, 425, 453
BspMAI CTGCAG 1 cut(s) 69
BspMI ACCTGC 1 cut(s) 439
BspT104I TTCGAA 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 451
BsrDI GCAATG 2 cut(s) 249, 393
BsrI ACTGG 2 cut(s) 449, 503
BssMI GATC 2 cut(s) 36, 105
Bst6I CTCTTC 3 cut(s) 180, 338, 465
BstAPI GCANNNNNTGC 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 2 cut(s) 315, 414
BstENI CCTNNNNNAGG 1 cut(s) 135
BstF5I GGATG 1 cut(s) 383
BstKTI GATC 2 cut(s) 39, 108
BstMAI GTCTC 2 cut(s) 86, 226
BstMBI GATC 2 cut(s) 36, 105
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 4 cut(s) 77, 86, 374, 431
BstSFI CTRYAG 1 cut(s) 65
BstSLI GKGCMC 1 cut(s) 427
BstV1I GCAGC 1 cut(s) 64
BstXI CCANNNNNNTGG 1 cut(s) 498
BsuRI GGCC 3 cut(s) 129, 425, 474
BtrI CACGTC 1 cut(s) 403
BtsCI GGATG 1 cut(s) 383
BveI ACCTGC 1 cut(s) 439
Cac8I GCNNGC 1 cut(s) 69
CaiI CAGNNNCTG 1 cut(s) 77
Cfr13I GGNCC 3 cut(s) 128, 423, 424
CviAII CATG 1 cut(s) 49
CviJI RGCY 9 cut(s) 8, 23, 42, 129, 319, 330, 368, 425, 474
CviKI_1 RGCY 9 cut(s) 8, 23, 42, 129, 319, 330, 368, 425, 474
DdeI CTNAG 2 cut(s) 315, 414
DpnI GATC 2 cut(s) 38, 107
DpnII GATC 2 cut(s) 36, 105
DrdI GACNNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 472
Eam1104I CTCTTC 3 cut(s) 180, 338, 465
EarI CTCTTC 3 cut(s) 180, 338, 465
Eco24I GRGCYC 1 cut(s) 427
EcoNI CCTNNNNNAGG 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 427
FaeI CATG 1 cut(s) 52
FatI CATG 1 cut(s) 48
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 1 cut(s) 370
FriOI GRGCYC 1 cut(s) 427
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 1 cut(s) 137
GluI GCNGC 1 cut(s) 78
HaeIII GGCC 3 cut(s) 129, 425, 474
HapII CCGG 1 cut(s) 225
Hin1II CATG 1 cut(s) 52
HinfI GANTC 1 cut(s) 285
HpaII CCGG 1 cut(s) 225
HphI GGTGA 1 cut(s) 187
Hpy188I TCNGA 2 cut(s) 30, 245
Hpy188III TCNNGA 1 cut(s) 225
Hpy99I CGWCG 2 cut(s) 113, 404
HpyAV CCTTC 1 cut(s) 338
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 5 cut(s) 67, 80, 302, 355, 377
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 86, 374, 431
HpyF3I CTNAG 2 cut(s) 315, 414
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 1 cut(s) 52
Kpn2I TCCGGA 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 2 cut(s) 36, 105
LmnI GCTCC 1 cut(s) 5
LpnPI CCDG 9 cut(s) 53, 134, 238, 267, 288, 354, 430, 434, 484
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 1 cut(s) 64
MaeI CTAG 1 cut(s) 137
MaeII ACGT 1 cut(s) 402
MalI GATC 2 cut(s) 38, 107
MboI GATC 2 cut(s) 36, 105
MboII GAAGA 7 cut(s) 37, 167, 170, 240, 243, 325, 452
MhlI GDGCHC 2 cut(s) 427, 436
MluCI AATT 3 cut(s) 306, 466, 503
MlyI GAGTC 1 cut(s) 279
MnlI CCTC 7 cut(s) 106, 183, 208, 341, 423, 465, 468
MroI TCCGGA 1 cut(s) 224
MseI TTAA 4 cut(s) 54, 297, 326, 523
MspI CCGG 1 cut(s) 225
MwoI GCNNNNNNNGC 4 cut(s) 77, 86, 374, 431
NdeII GATC 2 cut(s) 36, 105
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 3 cut(s) 247, 425, 453
NspV TTCGAA 1 cut(s) 162
PaqCI CACCTGC 1 cut(s) 439
PflFI GACNNNGTC 1 cut(s) 404
PkrI GCNGC 1 cut(s) 79
Ple19I CGATCG 1 cut(s) 108
PleI GAGTC 1 cut(s) 279
PpsI GAGTC 1 cut(s) 279
PshBI ATTAAT 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 247, 425, 453
PspOMI GGGCCC 1 cut(s) 423
PspPI GGNCC 3 cut(s) 128, 423, 424
PstI CTGCAG 1 cut(s) 69
PstNI CAGNNNCTG 1 cut(s) 77
PsyI GACNNNGTC 1 cut(s) 404
PvuI CGATCG 1 cut(s) 108
SaqAI TTAA 4 cut(s) 54, 297, 326, 523
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 2 cut(s) 36, 105
Sau96I GGNCC 3 cut(s) 128, 423, 424
SchI GAGTC 1 cut(s) 279
SduI GDGCHC 2 cut(s) 427, 436
SfaNI GCATC 1 cut(s) 64
SfcI CTRYAG 1 cut(s) 65
SfuI TTCGAA 1 cut(s) 162
Sse9I AATT 3 cut(s) 306, 466, 503
SsiI CCGC 1 cut(s) 87
SspMI CTAG 1 cut(s) 137
TaiI ACGT 1 cut(s) 405
TaqI TCGA 4 cut(s) 104, 108, 162, 336
TasI AATT 3 cut(s) 306, 466, 503
Tru1I TTAA 4 cut(s) 54, 297, 326, 523
Tru9I TTAA 4 cut(s) 54, 297, 326, 523
TseI GCWGC 1 cut(s) 77
TspDTI ATGAA 2 cut(s) 37, 182
Tth111I GACNNNGTC 1 cut(s) 404
VspI ATTAAT 1 cut(s) 297
XagI CCTNNNNNAGG 1 cut(s) 135
XapI RAATTY 1 cut(s) 503
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.