Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000801

Physical Location & Seq

Genomic Coordinates Forward (+)

4878862 .. 4883424

Gene Structure 4563 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1069200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2616 bp

ATGGCGGATTCAATTTCATCAGTGTGTAACCTAGCGGTTCTCGACAAGCTCAGCGAGGTCTCCATCAACGAAATCCTCGAAAGCTACAGCGGCTTCTGCGCCGCAACAGACACCCTTCTGACCGGCGCCGGCGACCTCTCCGCCGGTCAGCAGATCGTATCTCACGTGCACGGCCTCTGCAAGCACGGGCTCGAGTCCCTCCTCCGCGACTACTTCCTCGGCGCTCTCGAGCGAACCTTCGAGAAAAATGGCGCCTTGAAATTTTGGCGGCATTTCGAGGCGTACGACGGCGATGGTTCGATCGAGGAGGAAGTGTTCTACAACGCTCTGGAAGAAATAGCAATGGAAAAGCAGTACCAGGAGAAGTGCTTATTGATTCTGGTTCACGCTTTGCAGTCTTACAACCAAGGAAGCCATGATTCTGATGATTATAGAGCGCAGCTTTTTGCTAAGTACCAGTTATCAGTGTCTTCGGTTCTTATGGCAACTCTCCCTCGACATTTTCCAGAGGTACTCCACTGGTATTTTAAGGGAAGGTTGGAGGAACTGAGTACTATAATGGGCGGGGAATTCCCGCGTGACCACGATGAGGATGATGGTGCAGATGATATGGATGTGGATGACAAATGCAAAGTCTCATACAGGAGTGGACAGATGGAGATTGATGAGTGCTATCCCCAAGGCAGGTTCTTGGACAACAATAAATTGGTGAACAACATTGGGAAGGTTGTTCGCGATCTTAGAAGTCTCGGGTTTACATCTATGGCTGAAGATGCCTATGCCTCTGCTATATTTTTGTTTCTAAAGGCCAAAGTTCATGATCTTGCTGGTGATGATTACCGGGTTTCTTTTTTGGAGTCCATTAAGCGGTGGATACAGGCCGTGCCTCTTCAGTTCTTGCATGCTCTTCTTGCTTATCTTGGTGACACTGTTAGTTATGATAGTGCTTCATCTGGTTTCAAGTCACCTTTGGCTTCATGCCCTAATACATTTTATCCTGGAATCGACATTCCATCAGAAGGACTTGTTAGATGGCAGTTGCGACTTGAGTATTTTGCTTATGAAACTTTGCAAGATTTAAGAATAGCCAAGTTATTTGAGATTATTGTGGATTATCCTGACAGCTCTCCTGCGATTGAAGATTTGAAACAATGTCTTGAATACACTGGACAGCATTCTAAGCTCGTTGAGTCATTTATCACTGCACTGCGTTATCGTTTACTTACTGCAGGTGCCTCAACCAATGACATTTTGCATCAATATGTTTCCACGATTAAAGCACTCCGGACTATAGATCCAGCAGGTGTTTTCCTCGAATCAGTTGGTGAACCAATAAGGGACTATTTGAGGGGAAGGAAAGACACCATAAAGTGCATTGTGACCATGCTTACAGATGGCACTGGTGGGAATTCAAATGTGTCTGGAAACACTGGGGATAGCCTTCTTGAAGAATTAAACAGAGATGAAGAAAATCAAGAGAACACTGGTCTTGATGACGATTTCCACACTGATGACAAGCAAGCATGGATAAATGCGTTGCGCTGGGAGCCTGACCCTGTAGAAGCTGATCCCTTGAAGGGTAGCAGGAACCGAAGGAAGGTTGATATACTTGGGATGATTGTTGGCATAATTGGTTCAAAAGATCAACTCGTTAATGAGTACCGTGTTATGCTGGCTGAAAAGCTTCTCAACAAATCTGATTATGACATTGATACAGAGATACGTACTCTTGAGCTCCTCAAGATACATTTTGGAGAGAGCAGCATGCAGAAATGCGAGATTATGCTGAATGATCTGATTGATTCTAAGAGGACTAACAGCAATATTAAAGCAACCATAAGTCAGCCATCTCAAATGGGTACTGATCTGGGAGATAAAGGGGTGTCCATGGATAATTTTGATGCTACAATCATCTCTTCAAATTTCTGGCCTCAAATCCAGGATGAGTCCCTCAATGTACCTGGGCCTGTTGACCAACTACTATCTGATTATGCAAAGAGGTTTAATGAAATCAAGACACCCCGTAAACTATTATGGAAGAAAAGTCTTGGTACTGTCAAGTTGGAGTTGCAATTCGACAATAGGGCAGTGCAATTCATGGTTGCTCCCATACAAGCAGCAATTATAATGCAATTTCAAGATCAGACAAGCTGGACCTCTAAGGATCTTGCAGCTGCAATTGGGGTTCCAGTAGACATACTTAATCGCAGGATAAATTTCTGGATCAGCAAGGGAATTCTTGCAGAATCACCCGGTGCAGACTCTGAAGACCACATGTTTACACTAATGGAAGGCATGGTTGACACCGGTAAAAATGGTGGTAACAATGGGAGCATTGAGGATCTTGTAGTTGGTGATGAGGAGGGAGAGAGCTCTGTGGCTTCGGTAGAGGATCAACTGTGCAAGGAAATGACTGTGTATGAGAAATTTATCTTGGGGATGCTCACAAACTTCGGAAGCATGGCATTAGATCGAATTCATAATACTCTCAAGATGTTCTGTATAGCGGATCCACCTTACGACAAAACTCTCCAGCAGCTACAGAGTTTCTTATCGGGTCTAGTCTCCGAAGAGAAGTTAGAACTAAGAGATGGAATGTACGTTTTGAAAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000152 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005680 GO:0005819 GO:0005856 GO:0007275 GO:0008150 GO:0009553 GO:0009561 GO:0015630 GO:0019899 GO:0031461 GO:0031625 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166

Pfam Domains

Protein Families

ANAPC2 Cullin

Protein Analysis

872

Amino Acids

97.75

Weight (kDa)

4.73

Isoelectric Point (pI)

38.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_ANAPC2	PF25773	344 - 452	2.2e-41	ANAPC2-like, TPR repeats
Cullin_AB	PF26557	637 - 761	4.6e-16	Cullin alpha+beta domain
ANAPC2	PF08672	810 - 868	1.4e-23	Anaphase promoting complex (APC) subunit 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000801)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04660
fragaria_vesca	FvH4_5g29350 FvH4_5g29350 FvH4_5g29350 FvH4_5g29350 FvH4_5g29350
malus_domestica	MD13G1068900.v1.1 MD16G1069200.v1.1
prunus_persica	Prupe.1G035400_v2.0.a1 Prupe.1G035400_v2.0.a1 Prupe.1G191200_v2.0.a1
pyrus_communis	pycom16g06020
rosa_chinensis	RchiOBHm_Chr1g0327871 RchiOBHm_Chr2g0121271 RchiOBHm_Chr4g0439421 RchiOBHm_Chr7g0200631
rosa_laevigata	RLG00000006274 RLG00000011822 RLG00000018454 RLG00000034065 RLG00000034779 RLG00000035517
rosa_multiflora	Rmu_sc0002635.1_g000001 Rmu_sc0006782.1_g000010 Rmu_sc0037339.1_g000001
rosa_roxburghii	Rroxscaffold_159G00433030 Rroxscaffold_3G00237290 Rroxscaffold_3G00259010 Rroxscaffold_4G00283810 Rroxscaffold_5G00380330 Rroxscaffold_6G00397040 Rroxscaffold_7G00199350 Rroxscaffold_7G00199460 Rroxscaffold_7G00212760 Rroxscaffold_7G00213330
rosa_rugosa	Rorug01G0198100 Rorug03G0008800 Rorug04G0030200 Rorug04G0317800 Rorug04G0317900 Rorug04G0318000 Rorug04G0443500 Rorug05G0260700 Rorug05G0260800 Rorug05G0536400 Rorug05G0536500
rosa_samantha	Rh1BG117800 Rh2AG412900 Rh2BG241400 Rh2CG278400 Rh2CG398900 Rh2CG490800 Rh4AG369300 Rh4BG017200 Rh4BG381100 Rh4CG395700 Rh4DG375700 Rh4DG375800 Rh5AG534500 Rh5BG490100 Rh5CG514100 Rh5DG386800 Rh6BG137000 Rh6CG037000 Rh6DG121500 Rh7CG205400
rosa_wichuraiana	Rw3G022720 Rw4G031480 Rw6G043670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2126
AarI	CACCTGC	2 cut(s)	1220, 1292
Acc36I	ACCTGC	3 cut(s)	675, 1220, 1292
AccB1I	GGYRCC	3 cut(s)	125, 251, 1232
AccI	GTMKAC	1 cut(s)	2193
AccII	CGCG	3 cut(s)	207, 577, 735
AccIII	TCCGGA	1 cut(s)	1284
AclWI	GGATC	8 cut(s)	1289, 1562, 2172, 2231, 2349, 2400, 2504, 2517
AcsI	RAATTY	8 cut(s)	260, 569, 1408, 1921, 2215, 2235, 2426, 2475
AcuI	CTGAAG	3 cut(s)	789, 875, 2286
AcvI	CACGTG	1 cut(s)	166
AcyI	GRCGYC	2 cut(s)	126, 252
AdeI	CACNNNGTG	1 cut(s)	2255
AfiI	CCNNNNNNNGG	6 cut(s)	589, 684, 867, 1019, 1577, 1597
AflIII	ACRYGT	1 cut(s)	2274
AgeI	ACCGGT	1 cut(s)	2306
AjnI	CCWGG	4 cut(s)	357, 997, 1938, 1960
AjuI	GAANNNNNNNTTGG	2 cut(s)	2417, 2449
AloI	GAACNNNNNNTCC	2 cut(s)	299, 331
Alw21I	GWGCWC	3 cut(s)	171, 1737, 2375
Alw26I	GTCTC	4 cut(s)	64, 640, 752, 2569
Alw44I	GTGCAC	1 cut(s)	167
AlwI	GGATC	8 cut(s)	1289, 1562, 2172, 2231, 2349, 2400, 2504, 2517
AlwNI	CAGNNNCTG	1 cut(s)	2264
Ama87I	CYCGRG	3 cut(s)	191, 227, 749
Aor13HI	TCCGGA	1 cut(s)	1284
AoxI	GGCC	5 cut(s)	172, 807, 879, 1928, 1964
ApaLI	GTGCAC	1 cut(s)	167
ApeKI	GCWGC	6 cut(s)	439, 1761, 2117, 2171, 2174, 2536
ApoI	RAATTY	8 cut(s)	260, 569, 1408, 1921, 2215, 2235, 2426, 2475
ArsI	GACNNNNNNTTYG	2 cut(s)	618, 650
AsiGI	ACCGGT	1 cut(s)	2306
Asp700I	GAANNNNTTC	1 cut(s)	1683
AspLEI	GCGC	6 cut(s)	101, 128, 224, 254, 439, 1542
AspS9I	GGNCC	2 cut(s)	1964, 2154
AsuC2I	CCSGG	2 cut(s)	842, 2253
AsuHPI	GGTGA	7 cut(s)	721, 842, 935, 957, 1337, 2241, 2366
AvaI	CYCGRG	3 cut(s)	191, 227, 749
AvaII	GGWCC	1 cut(s)	2154
BaeGI	GKGCMC	1 cut(s)	171
BamHI	GGATCC	1 cut(s)	2509
BanI	GGYRCC	3 cut(s)	125, 251, 1232
BanII	GRGCYC	3 cut(s)	192, 1737, 2375
BbrPI	CACGTG	1 cut(s)	166
BbsI	GAAGAC	2 cut(s)	462, 2274
Bbv12I	GWGCWC	3 cut(s)	171, 1737, 2375
BbvI	GCAGC	6 cut(s)	451, 1773, 2129, 2161, 2183, 2548
BccI	CCATC	9 cut(s)	71, 287, 590, 649, 1021, 1026, 1388, 1855, 2585
BceAI	ACGGC	3 cut(s)	187, 304, 866
BciT130I	CCWGG	4 cut(s)	359, 999, 1940, 1962
BciVI	GTATCC	1 cut(s)	867
BcnI	CCSGG	2 cut(s)	842, 2253
BcoDI	GTCTC	4 cut(s)	64, 640, 752, 2569
BfaI	CTAG	2 cut(s)	32, 2561
BfmI	CTRYAG	5 cut(s)	85, 1227, 1290, 1557, 2540
BfoI	RGCGCY	3 cut(s)	129, 225, 255
BfuAI	ACCTGC	3 cut(s)	675, 1220, 1292
BfuI	GTATCC	1 cut(s)	867
BisI	GCNGC	9 cut(s)	91, 102, 269, 440, 1762, 2118, 2172, 2175, 2537
BlpI	GCTNAGC	1 cut(s)	50
BlsI	GCNGC	9 cut(s)	92, 103, 270, 441, 1763, 2119, 2173, 2176, 2538
BmcAI	AGTACT	1 cut(s)	553
Bme1390I	CCNGG	6 cut(s)	359, 842, 999, 1940, 1962, 2253
Bme18I	GGWCC	1 cut(s)	2154
BmeT110I	CYCGRG	3 cut(s)	191, 227, 749
BmgT120I	GGNCC	2 cut(s)	1964, 2154
BmiI	GGNNCC	7 cut(s)	127, 253, 1234, 1548, 1589, 2187, 2511
BmrFI	CCNGG	6 cut(s)	359, 842, 999, 1940, 1962, 2253
BmrI	ACTGGG	1 cut(s)	1440
BmsI	GCATC	4 cut(s)	763, 1264, 1891, 2430
BmuI	ACTGGG	1 cut(s)	1440
BpiI	GAAGAC	2 cut(s)	462, 2274
BpmI	CTGGAG	1 cut(s)	2516
Bpu1102I	GCTNAGC	1 cut(s)	50
BpuEI	CTTGAG	4 cut(s)	1067, 1724, 1751, 2474
BpuMI	CCSGG	2 cut(s)	842, 2253
BsaAI	YACGTR	2 cut(s)	166, 1724
BsaHI	GRCGYC	2 cut(s)	126, 252
BsaI	GGTCTC	1 cut(s)	64
BsaJI	CCNNGG	5 cut(s)	217, 406, 679, 1887, 1961
BsaWI	WCCGGW	2 cut(s)	1284, 2306
BsaXI	ACNNNNNCTCC	2 cut(s)	299, 329
Bsc4I	CCNNNNNNNGG	6 cut(s)	589, 684, 867, 1019, 1577, 1597
Bse118I	RCCGGY	4 cut(s)	122, 128, 143, 2306
Bse1I	ACTGG	7 cut(s)	457, 524, 1171, 1405, 1435, 1489, 2189
Bse3DI	GCAATG	1 cut(s)	348
BseAI	TCCGGA	1 cut(s)	1284
BseBI	CCWGG	4 cut(s)	359, 999, 1940, 1962
BseDI	CCNNGG	5 cut(s)	217, 406, 679, 1887, 1961
BseGI	GGATG	6 cut(s)	598, 619, 625, 1620, 1948, 2445
BseLI	CCNNNNNNNGG	6 cut(s)	589, 684, 867, 1019, 1577, 1597
BseMI	GCAATG	1 cut(s)	348
BseMII	CTCAG	2 cut(s)	64, 539
BseNI	ACTGG	7 cut(s)	457, 524, 1171, 1405, 1435, 1489, 2189
BseRI	GAGGAG	4 cut(s)	191, 320, 1727, 2375
BseSI	GKGCMC	1 cut(s)	171
BseXI	GCAGC	6 cut(s)	451, 1773, 2129, 2161, 2183, 2548
BseYI	CCCAGC	1 cut(s)	1542
BsgI	GTGCAG	3 cut(s)	621, 1188, 2277
Bsh1236I	CGCG	3 cut(s)	207, 577, 735
Bsh1285I	CGRYCG	1 cut(s)	303
BshFI	GGCC	5 cut(s)	174, 809, 881, 1930, 1966
BshNI	GGYRCC	3 cut(s)	125, 251, 1232
BshTI	ACCGGT	1 cut(s)	2306
BsiEI	CGRYCG	1 cut(s)	303
BsiHKAI	GWGCWC	3 cut(s)	171, 1737, 2375
BsiHKCI	CYCGRG	3 cut(s)	191, 227, 749
BsiSI	CCGG	7 cut(s)	123, 129, 144, 841, 1285, 2253, 2307
BsiWI	CGTACG	1 cut(s)	282
BslFI	GGGAC	3 cut(s)	181, 1352, 1933
BslI	CCNNNNNNNGG	6 cut(s)	589, 684, 867, 1019, 1577, 1597
BsmAI	GTCTC	4 cut(s)	64, 640, 752, 2569
BsmFI	GGGAC	3 cut(s)	181, 1352, 1933
BsmI	GAATGC	1 cut(s)	1174
BsnI	GGCC	5 cut(s)	174, 809, 881, 1930, 1966
Bso31I	GGTCTC	1 cut(s)	64
BsoBI	CYCGRG	3 cut(s)	191, 227, 749
Bsp1286I	GDGCHC	4 cut(s)	171, 192, 1737, 2375
Bsp13I	TCCGGA	1 cut(s)	1284
Bsp1720I	GCTNAGC	1 cut(s)	50
Bsp19I	CCATGG	1 cut(s)	1887
Bsp68I	TCGCGA	1 cut(s)	735
BspANI	GGCC	5 cut(s)	174, 809, 881, 1930, 1966
BspCNI	CTCAG	2 cut(s)	63, 540
BspEI	TCCGGA	1 cut(s)	1284
BspFNI	CGCG	3 cut(s)	207, 577, 735
BspHI	TCATGA	1 cut(s)	817
BspLI	GGNNCC	7 cut(s)	127, 253, 1234, 1548, 1589, 2187, 2511
BspMAI	CTGCAG	1 cut(s)	1231
BspMI	ACCTGC	3 cut(s)	675, 1220, 1292
BspPI	GGATC	8 cut(s)	1289, 1562, 2172, 2231, 2349, 2400, 2504, 2517
BspQI	GCTCTTC	1 cut(s)	912
BspT107I	GGYRCC	3 cut(s)	125, 251, 1232
BspTNI	GGTCTC	1 cut(s)	64
BsrDI	GCAATG	1 cut(s)	348
BsrFI	RCCGGY	4 cut(s)	122, 128, 143, 2306
BsrI	ACTGG	7 cut(s)	457, 524, 1171, 1405, 1435, 1489, 2189
BssAI	RCCGGY	4 cut(s)	122, 128, 143, 2306
BssECI	CCNNGG	5 cut(s)	217, 406, 679, 1887, 1961
BssNI	GRCGYC	2 cut(s)	126, 252
BssT1I	CCWWGG	3 cut(s)	406, 679, 1887
Bst2UI	CCWGG	4 cut(s)	359, 999, 1940, 1962
Bst4CI	ACNGT	5 cut(s)	931, 1664, 2056, 2400, 2416
Bst6I	CTCTTC	4 cut(s)	894, 912, 1921, 2565
BstACI	GRCGYC	2 cut(s)	126, 252
BstBAI	YACGTR	2 cut(s)	166, 1724
BstC8I	GCNNGC	6 cut(s)	130, 182, 903, 1521, 1674, 1766
BstDEI	CTNAG	8 cut(s)	50, 450, 548, 740, 1179, 1806, 2160, 2585
BstDSI	CCRYGG	1 cut(s)	1887
BstF5I	GGATG	6 cut(s)	598, 619, 625, 1620, 1948, 2445
BstFNI	CGCG	3 cut(s)	207, 577, 735
BstH2I	RGCGCY	3 cut(s)	129, 225, 255
BstHHI	GCGC	6 cut(s)	101, 128, 224, 254, 439, 1542
BstMAI	GTCTC	4 cut(s)	64, 640, 752, 2569
BstMCI	CGRYCG	1 cut(s)	303
BstMWI	GCNNNNNNNGC	6 cut(s)	90, 96, 773, 911, 1180, 1546
BstNI	CCWGG	4 cut(s)	359, 999, 1940, 1962
BstNSI	RCATGY	3 cut(s)	905, 1768, 2278
BstSCI	CCNGG	6 cut(s)	357, 840, 997, 1938, 1960, 2251
BstSFI	CTRYAG	5 cut(s)	85, 1227, 1290, 1557, 2540
BstSLI	GKGCMC	1 cut(s)	171
BstSNI	TACGTA	1 cut(s)	1724
BstUI	CGCG	3 cut(s)	207, 577, 735
BstV1I	GCAGC	6 cut(s)	451, 1773, 2129, 2161, 2183, 2548
BstV2I	GAAGAC	2 cut(s)	462, 2274
BstX2I	RGATCY	4 cut(s)	1294, 2164, 2341, 2509
BstYI	RGATCY	4 cut(s)	1294, 2164, 2341, 2509
BsuI	GTATCC	1 cut(s)	867
BsuRI	GGCC	5 cut(s)	174, 809, 881, 1930, 1966
BtgI	CCRYGG	1 cut(s)	1887
BtgZI	GCGATG	1 cut(s)	306
BtsCI	GGATG	6 cut(s)	598, 619, 625, 1620, 1948, 2445
BtsI	GCAGTG	3 cut(s)	1200, 1205, 2094
BtuMI	TCGCGA	1 cut(s)	735
BveI	ACCTGC	3 cut(s)	675, 1220, 1292
Cac8I	GCNNGC	6 cut(s)	130, 182, 903, 1521, 1674, 1766
CaiI	CAGNNNCTG	1 cut(s)	2264
CciI	TCATGA	1 cut(s)	817
CfoI	GCGC	6 cut(s)	101, 128, 224, 254, 439, 1542
Cfr10I	RCCGGY	4 cut(s)	122, 128, 143, 2306
Cfr13I	GGNCC	2 cut(s)	1964, 2154
CspAI	ACCGGT	1 cut(s)	2306
DdeI	CTNAG	8 cut(s)	50, 450, 548, 740, 1179, 1806, 2160, 2585
DinI	GGCGCC	2 cut(s)	127, 253
DraIII	CACNNNGTG	1 cut(s)	2255
Eam1104I	CTCTTC	4 cut(s)	894, 912, 1921, 2565
EarI	CTCTTC	4 cut(s)	894, 912, 1921, 2565
EciI	GGCGGA	2 cut(s)	20, 130
Ecl136II	GAGCTC	2 cut(s)	1735, 2373
Eco105I	TACGTA	1 cut(s)	1724
Eco130I	CCWWGG	3 cut(s)	406, 679, 1887
Eco24I	GRGCYC	3 cut(s)	192, 1737, 2375
Eco31I	GGTCTC	1 cut(s)	64
Eco47I	GGWCC	1 cut(s)	2154
Eco53kI	GAGCTC	2 cut(s)	1735, 2373
Eco57I	CTGAAG	3 cut(s)	789, 875, 2286
Eco72I	CACGTG	1 cut(s)	166
Eco88I	CYCGRG	3 cut(s)	191, 227, 749
EcoICRI	GAGCTC	2 cut(s)	1735, 2373
EcoRI	GAATTC	4 cut(s)	569, 1408, 2235, 2475
EcoRII	CCWGG	4 cut(s)	357, 997, 1938, 1960
EcoT14I	CCWWGG	3 cut(s)	406, 679, 1887
EcoT38I	GRGCYC	3 cut(s)	192, 1737, 2375
EgeI	GGCGCC	2 cut(s)	127, 253
EheI	GGCGCC	2 cut(s)	127, 253
ErhI	CCWWGG	3 cut(s)	406, 679, 1887
FaqI	GGGAC	3 cut(s)	181, 1352, 1933
FauI	CCCGC	2 cut(s)	557, 582
FblI	GTMKAC	1 cut(s)	2193
Fnu4HI	GCNGC	9 cut(s)	91, 102, 269, 440, 1762, 2118, 2172, 2175, 2537
FokI	GGATG	6 cut(s)	605, 626, 632, 1627, 1955, 2452
FriOI	GRGCYC	3 cut(s)	192, 1737, 2375
Fsp4HI	GCNGC	9 cut(s)	91, 102, 269, 440, 1762, 2118, 2172, 2175, 2537
FspBI	CTAG	2 cut(s)	32, 2561
GlaI	GCGC	6 cut(s)	100, 127, 223, 253, 438, 1541
GluI	GCNGC	9 cut(s)	91, 102, 269, 440, 1762, 2118, 2172, 2175, 2537
GsaI	CCCAGC	1 cut(s)	1546
GsuI	CTGGAG	1 cut(s)	2516
HaeII	RGCGCY	3 cut(s)	129, 225, 255
HaeIII	GGCC	5 cut(s)	174, 809, 881, 1930, 1966
HapII	CCGG	7 cut(s)	123, 129, 144, 841, 1285, 2253, 2307
HhaI	GCGC	6 cut(s)	101, 128, 224, 254, 439, 1542
Hin1I	GRCGYC	2 cut(s)	126, 252
Hin6I	GCGC	6 cut(s)	99, 126, 222, 252, 437, 1540
HinP1I	GCGC	6 cut(s)	99, 126, 222, 252, 437, 1540
HincII	GTYRAC	2 cut(s)	1972, 2302
HindII	GTYRAC	2 cut(s)	1972, 2302
HindIII	AAGCTT	1 cut(s)	1682
HpaII	CCGG	7 cut(s)	123, 129, 144, 841, 1285, 2253, 2307
HphI	GGTGA	7 cut(s)	721, 842, 935, 957, 1337, 2241, 2366
Hpy99I	CGWCG	1 cut(s)	290
HpyCH4III	ACNGT	5 cut(s)	931, 1664, 2056, 2400, 2416
HpyCH4IV	ACGT	3 cut(s)	165, 1723, 2601
HpyF10VI	GCNNNNNNNGC	6 cut(s)	90, 96, 773, 911, 1180, 1546
HpyF3I	CTNAG	8 cut(s)	50, 450, 548, 740, 1179, 1806, 2160, 2585
HpySE526I	ACGT	3 cut(s)	165, 1723, 2601
Hsp92I	GRCGYC	2 cut(s)	126, 252
HspAI	GCGC	6 cut(s)	99, 126, 222, 252, 437, 1540
KasI	GGCGCC	2 cut(s)	125, 251
Kpn2I	TCCGGA	1 cut(s)	1284
KroI	GCCGGC	1 cut(s)	128
KroNI	GCCGGC	1 cut(s)	130
LguI	GCTCTTC	1 cut(s)	912
LmnI	GCTCC	4 cut(s)	1546, 1740, 2110, 2331
Lsp1109I	GCAGC	6 cut(s)	451, 1773, 2129, 2161, 2183, 2548
LweI	GCATC	4 cut(s)	763, 1264, 1891, 2430
MaeI	CTAG	2 cut(s)	32, 2561
MaeII	ACGT	3 cut(s)	165, 1723, 2601
MaeIII	GTNAC	6 cut(s)	26, 578, 923, 963, 1378, 2321
MfeI	CAATTG	1 cut(s)	2178
MflI	RGATCY	4 cut(s)	1294, 2164, 2341, 2509
MhlI	GDGCHC	4 cut(s)	171, 192, 1737, 2375
Mly113I	GGCGCC	2 cut(s)	126, 252
MlyI	GAGTC	5 cut(s)	203, 866, 1199, 1955, 2255
MmeI	TCCRAC	2 cut(s)	519, 2043
MreI	CGCCGGCG	1 cut(s)	128
MroI	TCCGGA	1 cut(s)	1284
MroNI	GCCGGC	1 cut(s)	128
MroXI	GAANNNNTTC	1 cut(s)	1683
MseI	TTAA	9 cut(s)	528, 864, 1079, 1275, 1454, 1653, 1827, 2004, 2202
MslI	CAYNNNNRTG	3 cut(s)	22, 1260, 1509
MspA1I	CMGCKG	2 cut(s)	90, 2174
MspI	CCGG	7 cut(s)	123, 129, 144, 841, 1285, 2253, 2307
MspR9I	CCNGG	6 cut(s)	359, 842, 999, 1940, 1962, 2253
MunI	CAATTG	1 cut(s)	2178
Mva1269I	GAATGC	1 cut(s)	1174
MvaI	CCWGG	4 cut(s)	359, 999, 1940, 1962
MvnI	CGCG	3 cut(s)	207, 577, 735
MwoI	GCNNNNNNNGC	6 cut(s)	90, 96, 773, 911, 1180, 1546
NaeI	GCCGGC	1 cut(s)	130
NarI	GGCGCC	2 cut(s)	126, 252
NciI	CCSGG	2 cut(s)	842, 2253
NcoI	CCATGG	1 cut(s)	1887
NgoMIV	GCCGGC	1 cut(s)	128
NlaIV	GGNNCC	7 cut(s)	127, 253, 1234, 1548, 1589, 2187, 2511
NmeAIII	GCCGAG	1 cut(s)	198
NmuCI	GTSAC	4 cut(s)	578, 923, 963, 1378
NruI	TCGCGA	1 cut(s)	735
NspI	RCATGY	3 cut(s)	905, 1768, 2278
PaeI	GCATGC	2 cut(s)	905, 1768
PaeR7I	CTCGAG	2 cut(s)	191, 227
PagI	TCATGA	1 cut(s)	817
PaqCI	CACCTGC	2 cut(s)	1220, 1292
PciI	ACATGT	1 cut(s)	2274
PciSI	GCTCTTC	1 cut(s)	912
PcsI	WCGNNNNNNNCGW	4 cut(s)	75, 162, 225, 282
PctI	GAATGC	1 cut(s)	1174
PdiI	GCCGGC	1 cut(s)	130
PdmI	GAANNNNTTC	1 cut(s)	1683
PfeI	GAWTC	7 cut(s)	8, 376, 419, 1002, 1316, 1802, 2246
Pfl23II	CGTACG	1 cut(s)	282
PfoI	TCCNGGA	2 cut(s)	997, 1938
PinAI	ACCGGT	1 cut(s)	2306
PkrI	GCNGC	9 cut(s)	92, 103, 270, 441, 1763, 2119, 2173, 2176, 2538
Ple19I	CGATCG	1 cut(s)	303
PleI	GAGTC	5 cut(s)	202, 865, 1198, 1954, 2255
PluTI	GGCGCC	2 cut(s)	129, 255
PmaCI	CACGTG	1 cut(s)	166
PmlI	CACGTG	1 cut(s)	166
PpsI	GAGTC	5 cut(s)	202, 865, 1198, 1954, 2255
Ppu21I	YACGTR	2 cut(s)	166, 1724
PscI	ACATGT	1 cut(s)	2274
PsiI	TTATAA	1 cut(s)	2126
Psp124BI	GAGCTC	2 cut(s)	1737, 2375
Psp6I	CCWGG	4 cut(s)	357, 997, 1938, 1960
PspCI	CACGTG	1 cut(s)	166
PspFI	CCCAGC	1 cut(s)	1542
PspGI	CCWGG	4 cut(s)	357, 997, 1938, 1960
PspLI	CGTACG	1 cut(s)	282
PspN4I	GGNNCC	7 cut(s)	127, 253, 1234, 1548, 1589, 2187, 2511
PspPI	GGNCC	2 cut(s)	1964, 2154
PspXI	VCTCGAGB	1 cut(s)	191
PstI	CTGCAG	1 cut(s)	1231
PstNI	CAGNNNCTG	1 cut(s)	2264
PsuI	RGATCY	4 cut(s)	1294, 2164, 2341, 2509
PvuI	CGATCG	1 cut(s)	303
PvuII	CAGCTG	1 cut(s)	2174
RruI	TCGCGA	1 cut(s)	735
RseI	CAYNNNNRTG	3 cut(s)	22, 1260, 1509
SacI	GAGCTC	2 cut(s)	1737, 2375
SapI	GCTCTTC	1 cut(s)	912
SaqAI	TTAA	9 cut(s)	528, 864, 1079, 1275, 1454, 1653, 1827, 2004, 2202
SatI	GCNGC	9 cut(s)	91, 102, 269, 440, 1762, 2118, 2172, 2175, 2537
Sau96I	GGNCC	2 cut(s)	1964, 2154
ScaI	AGTACT	1 cut(s)	553
SchI	GAGTC	5 cut(s)	203, 866, 1199, 1955, 2255
ScrFI	CCNGG	6 cut(s)	359, 842, 999, 1940, 1962, 2253
SduI	GDGCHC	4 cut(s)	171, 192, 1737, 2375
SfaNI	GCATC	4 cut(s)	763, 1264, 1891, 2430
SfcI	CTRYAG	5 cut(s)	85, 1227, 1290, 1557, 2540
SfoI	GGCGCC	2 cut(s)	127, 253
Sfr274I	CTCGAG	2 cut(s)	191, 227
SgrAI	CRCCGGYG	1 cut(s)	128
SinI	GGWCC	1 cut(s)	2154
SlaI	CTCGAG	2 cut(s)	191, 227
SmiMI	CAYNNNNRTG	3 cut(s)	22, 1260, 1509
SmlI	CTYRAG	6 cut(s)	191, 227, 1046, 1730, 1739, 2489
SmoI	CTYRAG	6 cut(s)	191, 227, 1046, 1730, 1739, 2489
SnaBI	TACGTA	1 cut(s)	1724
SphI	GCATGC	2 cut(s)	905, 1768
SspDI	GGCGCC	2 cut(s)	125, 251
SspI	AATATT	1 cut(s)	1825
SspMI	CTAG	2 cut(s)	32, 2561
SstI	GAGCTC	2 cut(s)	1737, 2375
StyD4I	CCNGG	6 cut(s)	357, 840, 997, 1938, 1960, 2251
StyI	CCWWGG	3 cut(s)	406, 679, 1887
TaaI	ACNGT	5 cut(s)	931, 1664, 2056, 2400, 2416
TaiI	ACGT	3 cut(s)	168, 1726, 2604
TatI	WGTACW	1 cut(s)	551
TauI	GCSGC	3 cut(s)	93, 104, 271
TfiI	GAWTC	7 cut(s)	8, 376, 419, 1002, 1316, 1802, 2246
Tru1I	TTAA	9 cut(s)	528, 864, 1079, 1275, 1454, 1653, 1827, 2004, 2202
Tru9I	TTAA	9 cut(s)	528, 864, 1079, 1275, 1454, 1653, 1827, 2004, 2202
TseFI	GTSAC	4 cut(s)	578, 923, 963, 1378
TseI	GCWGC	6 cut(s)	439, 1761, 2117, 2171, 2174, 2536
Tsp45I	GTSAC	4 cut(s)	578, 923, 963, 1378
TspDTI	ATGAA	9 cut(s)	6, 806, 939, 966, 1077, 1479, 2022, 2086, 2468
VneI	GTGCAC	1 cut(s)	167
VpaK11BI	GGWCC	1 cut(s)	2154
XapI	RAATTY	8 cut(s)	260, 569, 1408, 1921, 2215, 2235, 2426, 2475
XceI	RCATGY	3 cut(s)	905, 1768, 2278
XhoI	CTCGAG	2 cut(s)	191, 227
XmiI	GTMKAC	1 cut(s)	2193
XmnI	GAANNNNTTC	1 cut(s)	1683
XspI	CTAG	2 cut(s)	32, 2561
ZrmI	AGTACT	1 cut(s)	553

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.