Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0000801

Physical Location & Seq

Genomic Coordinates Forward (+)

67759309 .. 67764599

Gene Structure 5291 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG369300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2622 bp

ATGGCGGATTCGATTTCATCAACCTGCAATCTAGGGTTTCTCGACACACTCAGCGAAACCTCCATCCACGAAATCGTCGAAAGCTACTCCGCCTTCTGCGCCGCCACCGAAACCCTCCTCAACGGCGCCGGAGACCTCTCCGTGGGCCCGCAACTCGTCTCCCACGTCCACCGCCTCTGCAGCCACGGCCTCGAGTCTCTCGTCCGCGACCACTTCCTCGGCGCGCTCGAGAGAACCTTCGAGAAAGACGGCGCCTCCAAATTCTGGCGGCATTTCGAGGCACACGACGACGTTTCGATCGGAGAGCAAGTGTTCTACAACGCCTTGGAGGAAATCTCACTGGAAAAGCAGTACCAGGAGAAGTGCTTGCTGATGCTGGTTCATGCTTTACAGACTTATGGAGGAAGAAGAAGCCGTGATTCCGATGACGTCAGCCCGGAGCTCTTTGCTAAGTATCAATTATCAGTGTCCTCGATTCTTATGGCCACTCTTCCTCGGAAATTTCCAGACATACTCAACTGGTATTTTAAGGGAAGGTTGGAGGAACTGAGTACTATAATGGCCGGAGACGAGCCTCGTGGCGATGAGGATGAAGATGACAGAGATGATATGGAGTTGGATGAGAAATGCAGAGTGTCATACAGGAGTGGACAAATGGAGATTGATGAATGTAATCCCCAGGGGAGGTTCTTTGATAATAATAAATTGGTCAACAACATAGGGCGGGTTGTACATGATTTGAGGAGTCTTGGATTTACATCTATGACTGAAGATGCATATGCATCTGCTATCTTTTTGTTTCTTAAGGACAAAGTTCATGACCTTGCTGGTGATGATTATCGGATTTCTGTTTTGGAGTCTATTAAGAGGTGGATACAGGCTGTGCCTCTTCAGTTCTTGCATGCTTTACTTGCTTATCTTGGTGATTCTGTTAGTTATGAAAGTGCATCATCCGGTCTCAAGTCACCTTTGGCTAAATGCCCTGCTTCATTCTATCCTGGAATCGATACTCCATCTGAAGGTCTTGTAAGATGGCAGTTGCGACTAGAGTATTTTGCTTATGAAACATTGCAAGATTTGAGAATAGCAAAATTATTTGAGATTATTGTGGATTATCCTGACAGCTCTCCTGCGATTGAAGATTTGAAACAGTGTCTTGAATACACTGGACAACATTCTAAGCTTGTGGAGTCATTTATTTCAGCACTACAGTACCGGTTGCTCACTGCAGGTGCCTCAACAAATGACATTTTGCATCAATATGTTTCAACGATTAAGGCACTCCGGACAATAGATCCAGCAGGTGTTTTCCTTGAAGCAGTCGGTGAGCCAATAAGGGACTATTTAAGGGGAAGGAAAGACACCATAAAGTGCATTGTGACCATGCTTACTGATGGGACTGGTGCAAATTCTAATGTATCCGGAAACACTGGCGATAGCCTTCTTGAAGAATTAAATAGAGATGAAGAAAGCCAAGAGAACATTGCTCCTGAGGATGACTTTCATACTGATGATAAGCAAGCTTGGATAAATGCTTCACGCTGGGAGCCTGACCCTGTAGAAGCTGACCCCTTGAAAGGTAGCAGGAACCGAAGGAAGGTTGACATACTTGGAATGATTGTTGGCATAATTGGTTCACAAGACCAACTTGTTAATGAATACCGTGTTATGCTGGCCGAAAAGCTTCTCAACAAATCTGATTATGACATTGATTCAGAGATACGTACTCTTGAACTCCTCAAGATACATTTTGGAGAGAACAGCATGCAGAAATGTGAAATTATGCTCAATGATCTGATTGGTTCTAAGAGGACCAACACTAATATTAAAGCAACCATAGCTCAGCCATCTCATACAGATGCAGGCGGTGAGCTGGGGGATAACAGGGTATCCATGGATATTCTTGACGCTACAATTATCTCTTCAAATTTTTGGCCTCCAATCCAGGAGGAGTCCCTAAATGTACCTGGGCCTGTTGACCAACTACTCTCTGATTATGCAAAGAGATTTAATGAAATCAAGACCCCTCGTAAGCTATTGTGGAAGAAGAATCTTGGTACTGTCAAGTTGGAATTGCAATTTGAAGATAGAGAAATGCAGTTCATGGTTGCTCCTCTTCATGCAGCAATCGTAATGCAATTTCAAGATCAGAAAAGCTGGACCTCCAAAGATCTTGCAGCTGCAGTTGGGGTTCCAATAGATATACTTAATCGGAGGATCAATTTTTGGATAAGCAAGGGAATTCTTGCAGAATCACTTGGGGAAGACACTGATGACCATGTGTTTACACTAATGGAAGGGATGATTGACACTGGCAAAAACACTGCTAATGATGGGAACTGCGAGGATCTTATAGTTGGTGATGAGGAAGGAGAGAGCTCTGTAGCCTCTGTAGAGGATCAACTGCGCAAGGAAATGACTGTGTATGAGAAATTTATTTTGGGGATGCTCACAAACTTTGGAAGCATGGCTTTGGATCGTATTCATAATACTCTCAAGATGTTCTGCATAGCTGATCCTACTTATGACAAAACCCTCCAACAGCTGCAAAGTTTCTTATCAGGTCTAGTTTCTGAAGAGAAATTAGAACTAAGAGATGGCATGTACTTTTTGAAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000152 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005680 GO:0005819 GO:0005856 GO:0007275 GO:0008150 GO:0009553 GO:0009561 GO:0015630 GO:0019899 GO:0031461 GO:0031625 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166

Pfam Domains

Protein Families

ANAPC2 Cullin

Protein Analysis

873

Amino Acids

98.3

Weight (kDa)

4.74

Isoelectric Point (pI)

39.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_ANAPC2	PF25773	344 - 452	9.5e-42	ANAPC2-like, TPR repeats
Cullin_AB	PF26557	639 - 752	1.4e-14	Cullin alpha+beta domain
ANAPC2	PF08672	812 - 869	2e-23	Anaphase promoting complex (APC) subunit 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000801)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04660
fragaria_vesca	FvH4_5g29350 FvH4_5g29350 FvH4_5g29350 FvH4_5g29350 FvH4_5g29350
malus_domestica	MD13G1068900.v1.1 MD16G1069200.v1.1
prunus_persica	Prupe.1G035400_v2.0.a1 Prupe.1G035400_v2.0.a1 Prupe.1G191200_v2.0.a1
pyrus_communis	pycom16g06020
rosa_chinensis	RchiOBHm_Chr1g0327871 RchiOBHm_Chr2g0121271 RchiOBHm_Chr4g0439421 RchiOBHm_Chr7g0200631
rosa_laevigata	RLG00000006274 RLG00000011822 RLG00000018454 RLG00000034065 RLG00000034779 RLG00000035517
rosa_multiflora	Rmu_sc0002635.1_g000001 Rmu_sc0006782.1_g000010 Rmu_sc0037339.1_g000001
rosa_roxburghii	Rroxscaffold_159G00433030 Rroxscaffold_3G00237290 Rroxscaffold_3G00259010 Rroxscaffold_4G00283810 Rroxscaffold_5G00380330 Rroxscaffold_6G00397040 Rroxscaffold_7G00199350 Rroxscaffold_7G00199460 Rroxscaffold_7G00212760 Rroxscaffold_7G00213330
rosa_rugosa	Rorug01G0198100 Rorug03G0008800 Rorug04G0030200 Rorug04G0317800 Rorug04G0317900 Rorug04G0318000 Rorug04G0443500 Rorug05G0260700 Rorug05G0260800 Rorug05G0536400 Rorug05G0536500
rosa_samantha	Rh1BG117800 Rh2AG412900 Rh2BG241400 Rh2CG278400 Rh2CG398900 Rh2CG490800 Rh4AG369300 Rh4BG017200 Rh4BG381100 Rh4CG395700 Rh4DG375700 Rh4DG375800 Rh5AG534500 Rh5BG490100 Rh5CG514100 Rh5DG386800 Rh6BG137000 Rh6CG037000 Rh6DG121500 Rh7CG205400
rosa_wichuraiana	Rw3G022720 Rw4G031480 Rw6G043670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1220, 1292
AatII	GACGTC	1 cut(s)	432
Acc16I	TGCGCA	1 cut(s)	2408
Acc36I	ACCTGC	3 cut(s)	32, 1220, 1292
AccB1I	GGYRCC	3 cut(s)	125, 251, 1232
AccB7I	CCANNNNNTGG	1 cut(s)	264
AccII	CGCG	2 cut(s)	207, 224
AccIII	TCCGGA	2 cut(s)	1284, 1421
AciI	CCGC	9 cut(s)	5, 90, 102, 149, 172, 205, 268, 724, 1866
AclWI	GGATC	6 cut(s)	1289, 2225, 2355, 2406, 2484, 2510
AcoI	YGGCCR	3 cut(s)	483, 561, 1674
AcsI	RAATTY	6 cut(s)	260, 500, 1408, 1927, 2241, 2432
AcuI	CTGAAG	4 cut(s)	789, 875, 1038, 2595
AcyI	GRCGYC	3 cut(s)	126, 252, 429
AfaI	GTAC	8 cut(s)	353, 553, 732, 1214, 1726, 1965, 2059, 2606
AfiI	CCNNNNNNNGG	6 cut(s)	142, 264, 684, 1019, 1577, 1597
AflII	CTTAAG	1 cut(s)	803
AgeI	ACCGGT	1 cut(s)	1215
AjiI	CACGTC	1 cut(s)	166
AjnI	CCWGG	5 cut(s)	354, 678, 997, 1944, 1966
AjuI	GAANNNNNNNTTGG	2 cut(s)	2423, 2455
Alw21I	GWGCWC	2 cut(s)	444, 2381
Alw26I	GTCTC	5 cut(s)	126, 163, 201, 561, 962
AlwI	GGATC	6 cut(s)	1289, 2225, 2355, 2406, 2484, 2510
Ama87I	CYCGRG	2 cut(s)	191, 227
Aor13HI	TCCGGA	2 cut(s)	1284, 1421
AoxI	GGCC	7 cut(s)	145, 187, 483, 561, 1674, 1934, 1970
ApaI	GGGCCC	1 cut(s)	149
ApeKI	GCWGC	5 cut(s)	180, 2123, 2177, 2180, 2545
ApoI	RAATTY	6 cut(s)	260, 500, 1408, 1927, 2241, 2432
AsiGI	ACCGGT	1 cut(s)	1215
Asp700I	GAANNNNTTC	1 cut(s)	1683
AspLEI	GCGC	6 cut(s)	101, 128, 224, 226, 254, 2409
AspS9I	GGNCC	5 cut(s)	145, 146, 1812, 1970, 2160
AsuC2I	CCSGG	1 cut(s)	437
AsuHPI	GGTGA	6 cut(s)	842, 935, 957, 1337, 1880, 2372
AvaI	CYCGRG	2 cut(s)	191, 227
AvaII	GGWCC	2 cut(s)	1812, 2160
AxyI	CCTNAGG	1 cut(s)	1491
BaeGI	GKGCMC	1 cut(s)	149
BaeI	ACNNNNGTAYC	2 cut(s)	1196, 1229
BalI	TGGCCA	1 cut(s)	485
BanI	GGYRCC	3 cut(s)	125, 251, 1232
BanII	GRGCYC	3 cut(s)	149, 444, 2381
BauI	CACGAG	1 cut(s)	576
BbsI	GAAGAC	1 cut(s)	2271
Bbv12I	GWGCWC	2 cut(s)	444, 2381
BbvI	GCAGC	5 cut(s)	192, 2135, 2167, 2189, 2532
BccI	CCATC	7 cut(s)	71, 1021, 1026, 1388, 1855, 2327, 2591
BceAI	ACGGC	4 cut(s)	139, 202, 265, 399
BciT130I	CCWGG	5 cut(s)	356, 680, 999, 1946, 1968
BciVI	GTATCC	3 cut(s)	867, 1429, 1900
BcnI	CCSGG	1 cut(s)	437
BcoDI	GTCTC	5 cut(s)	126, 163, 201, 561, 962
BfaI	CTAG	3 cut(s)	32, 1046, 2567
BfmI	CTRYAG	7 cut(s)	178, 1208, 1227, 1557, 2181, 2382, 2391
BfoI	RGCGCY	2 cut(s)	129, 255
BfrI	CTTAAG	1 cut(s)	803
BfuAI	ACCTGC	3 cut(s)	32, 1220, 1292
BfuI	GTATCC	3 cut(s)	867, 1429, 1900
BglII	AGATCT	1 cut(s)	2170
BisI	GCNGC	7 cut(s)	102, 181, 269, 2124, 2178, 2181, 2546
BlpI	GCTNAGC	1 cut(s)	1842
BlsI	GCNGC	7 cut(s)	103, 182, 270, 2125, 2179, 2182, 2547
BmcAI	AGTACT	1 cut(s)	553
Bme1390I	CCNGG	6 cut(s)	356, 437, 680, 999, 1946, 1968
Bme18I	GGWCC	2 cut(s)	1812, 2160
BmeT110I	CYCGRG	2 cut(s)	191, 227
BmgBI	CACGTC	1 cut(s)	166
BmgT120I	GGNCC	5 cut(s)	145, 146, 1812, 1970, 2160
BmiI	GGNNCC	7 cut(s)	127, 147, 253, 1234, 1548, 1589, 2193
BmrFI	CCNGG	6 cut(s)	356, 437, 680, 999, 1946, 1968
BmsI	GCATC	7 cut(s)	363, 763, 791, 956, 1264, 1849, 2436
BpiI	GAAGAC	1 cut(s)	2271
BplI	GAGNNNNNCTC	2 cut(s)	320, 352
Bpu1102I	GCTNAGC	1 cut(s)	1842
BpuEI	CTTGAG	3 cut(s)	944, 1724, 2480
BpuMI	CCSGG	1 cut(s)	437
Bsa29I	ATCGAT	1 cut(s)	1005
BsaAI	YACGTR	1 cut(s)	1724
BsaBI	GATNNNNATC	2 cut(s)	757, 837
BsaHI	GRCGYC	3 cut(s)	126, 252, 429
BsaI	GGTCTC	2 cut(s)	126, 962
BsaJI	CCNNGG	9 cut(s)	141, 184, 217, 324, 494, 678, 679, 1893, 1967
BsaWI	WCCGGW	4 cut(s)	953, 1215, 1284, 1421
BsaXI	ACNNNNNCTCC	4 cut(s)	239, 269, 294, 324
Bsc4I	CCNNNNNNNGG	6 cut(s)	142, 264, 684, 1019, 1577, 1597
Bse118I	RCCGGY	1 cut(s)	1215
Bse1I	ACTGG	6 cut(s)	345, 524, 1171, 1405, 1435, 2317
Bse21I	CCTNAGG	1 cut(s)	1491
Bse3DI	GCAATG	2 cut(s)	1067, 1482
Bse8I	GATNNNNATC	2 cut(s)	757, 837
BseAI	TCCGGA	2 cut(s)	1284, 1421
BseBI	CCWGG	5 cut(s)	356, 680, 999, 1946, 1968
BseCI	ATCGAT	1 cut(s)	1005
BseDI	CCNNGG	9 cut(s)	141, 184, 217, 324, 494, 678, 679, 1893, 1967
BseGI	GGATG	7 cut(s)	63, 595, 625, 950, 1501, 2307, 2451
BseJI	GATNNNNATC	2 cut(s)	757, 837
BseLI	CCNNNNNNNGG	6 cut(s)	142, 264, 684, 1019, 1577, 1597
BseMI	GCAATG	2 cut(s)	1067, 1482
BseMII	CTCAG	4 cut(s)	64, 539, 1482, 1856
BseNI	ACTGG	6 cut(s)	345, 524, 1171, 1405, 1435, 2317
BsePI	GCGCGC	1 cut(s)	222
BseRI	GAGGAG	5 cut(s)	107, 757, 1727, 1964, 2103
BseSI	GKGCMC	1 cut(s)	149
BseXI	GCAGC	5 cut(s)	192, 2135, 2167, 2189, 2532
BseYI	CCCAGC	2 cut(s)	1542, 1873
Bsh1236I	CGCG	2 cut(s)	207, 224
Bsh1285I	CGRYCG	1 cut(s)	300
BshFI	GGCC	7 cut(s)	147, 189, 485, 563, 1676, 1936, 1972
BshNI	GGYRCC	3 cut(s)	125, 251, 1232
BshTI	ACCGGT	1 cut(s)	1215
BshVI	ATCGAT	1 cut(s)	1005
BsiEI	CGRYCG	1 cut(s)	300
BsiHKAI	GWGCWC	2 cut(s)	444, 2381
BsiHKCI	CYCGRG	2 cut(s)	191, 227
BsiSI	CCGG	7 cut(s)	129, 437, 564, 954, 1216, 1285, 1422
BslFI	GGGAC	3 cut(s)	1352, 1411, 1939
BslI	CCNNNNNNNGG	6 cut(s)	142, 264, 684, 1019, 1577, 1597
BsmAI	GTCTC	5 cut(s)	126, 163, 201, 561, 962
BsmBI	CGTCTC	2 cut(s)	163, 561
BsmFI	GGGAC	3 cut(s)	1352, 1411, 1939
BsnI	GGCC	7 cut(s)	147, 189, 485, 563, 1676, 1936, 1972
Bso31I	GGTCTC	2 cut(s)	126, 962
BsoBI	CYCGRG	2 cut(s)	191, 227
Bsp120I	GGGCCC	1 cut(s)	145
Bsp1286I	GDGCHC	3 cut(s)	149, 444, 2381
Bsp13I	TCCGGA	2 cut(s)	1284, 1421
Bsp1407I	TGTACA	1 cut(s)	730
Bsp1720I	GCTNAGC	1 cut(s)	1842
Bsp19I	CCATGG	1 cut(s)	1893
BspACI	CCGC	9 cut(s)	5, 90, 102, 149, 172, 205, 268, 724, 1866
BspANI	GGCC	7 cut(s)	147, 189, 485, 563, 1676, 1936, 1972
BspCNI	CTCAG	4 cut(s)	63, 540, 1483, 1855
BspDI	ATCGAT	1 cut(s)	1005
BspEI	TCCGGA	2 cut(s)	1284, 1421
BspFNI	CGCG	2 cut(s)	207, 224
BspHI	TCATGA	1 cut(s)	817
BspLI	GGNNCC	7 cut(s)	127, 147, 253, 1234, 1548, 1589, 2193
BspMAI	CTGCAG	3 cut(s)	182, 1231, 2185
BspMI	ACCTGC	3 cut(s)	32, 1220, 1292
BspPI	GGATC	6 cut(s)	1289, 2225, 2355, 2406, 2484, 2510
BspT107I	GGYRCC	3 cut(s)	125, 251, 1232
BspTI	CTTAAG	1 cut(s)	803
BspTNI	GGTCTC	2 cut(s)	126, 962
BsrDI	GCAATG	2 cut(s)	1067, 1482
BsrFI	RCCGGY	1 cut(s)	1215
BsrGI	TGTACA	1 cut(s)	730
BsrI	ACTGG	6 cut(s)	345, 524, 1171, 1405, 1435, 2317
BssAI	RCCGGY	1 cut(s)	1215
BssECI	CCNNGG	9 cut(s)	141, 184, 217, 324, 494, 678, 679, 1893, 1967
BssHII	GCGCGC	1 cut(s)	222
BssNI	GRCGYC	3 cut(s)	126, 252, 429
BssSI	CACGAG	1 cut(s)	576
BssT1I	CCWWGG	2 cut(s)	324, 1893
Bst2BI	CACGAG	1 cut(s)	576
Bst2UI	CCWGG	5 cut(s)	356, 680, 999, 1946, 1968
Bst4CI	ACNGT	5 cut(s)	1152, 1212, 1664, 2062, 2422
Bst6I	CTCTTC	5 cut(s)	495, 894, 1927, 2121, 2571
BstACI	GRCGYC	3 cut(s)	126, 252, 429
BstAFI	CTTAAG	1 cut(s)	803
BstAUI	TGTACA	1 cut(s)	730
BstBAI	YACGTR	1 cut(s)	1724
BstC8I	GCNNGC	8 cut(s)	149, 224, 368, 903, 1521, 1674, 1766, 1864
BstDEI	CTNAG	8 cut(s)	50, 450, 548, 1179, 1491, 1806, 1842, 2591
BstDSI	CCRYGG	3 cut(s)	141, 184, 1893
BstF5I	GGATG	7 cut(s)	63, 595, 625, 950, 1501, 2307, 2451
BstFNI	CGCG	2 cut(s)	207, 224
BstH2I	RGCGCY	2 cut(s)	129, 255
BstHHI	GCGC	6 cut(s)	101, 128, 224, 226, 254, 2409
BstMAI	GTCTC	5 cut(s)	126, 163, 201, 561, 962
BstMCI	CGRYCG	1 cut(s)	300
BstMWI	GCNNNNNNNGC	5 cut(s)	98, 180, 186, 911, 1838
BstNI	CCWGG	5 cut(s)	356, 680, 999, 1946, 1968
BstNSI	RCATGY	3 cut(s)	905, 1768, 2605
BstSCI	CCNGG	6 cut(s)	354, 435, 678, 997, 1944, 1966
BstSFI	CTRYAG	7 cut(s)	178, 1208, 1227, 1557, 2181, 2382, 2391
BstSLI	GKGCMC	1 cut(s)	149
BstSNI	TACGTA	1 cut(s)	1724
BstUI	CGCG	2 cut(s)	207, 224
BstV1I	GCAGC	5 cut(s)	192, 2135, 2167, 2189, 2532
BstV2I	GAAGAC	1 cut(s)	2271
BstX2I	RGATCY	3 cut(s)	1294, 2170, 2347
BstYI	RGATCY	3 cut(s)	1294, 2170, 2347
Bsu15I	ATCGAT	1 cut(s)	1005
Bsu36I	CCTNAGG	1 cut(s)	1491
BsuI	GTATCC	3 cut(s)	867, 1429, 1900
BsuRI	GGCC	7 cut(s)	147, 189, 485, 563, 1676, 1936, 1972
BsuTUI	ATCGAT	1 cut(s)	1005
BtgI	CCRYGG	3 cut(s)	141, 184, 1893
BtgZI	GCGATG	1 cut(s)	597
BtrI	CACGTC	1 cut(s)	166
BtsCI	GGATG	7 cut(s)	63, 595, 625, 950, 1501, 2307, 2451
BtsI	GCAGTG	2 cut(s)	1224, 2322
BtsIMutI	CAGTG	9 cut(s)	338, 471, 1157, 1164, 1224, 1428, 2268, 2310, 2322
BveI	ACCTGC	3 cut(s)	32, 1220, 1292
Cac8I	GCNNGC	8 cut(s)	149, 224, 368, 903, 1521, 1674, 1766, 1864
CciI	TCATGA	1 cut(s)	817
CfoI	GCGC	6 cut(s)	101, 128, 224, 226, 254, 2409
Cfr10I	RCCGGY	1 cut(s)	1215
Cfr13I	GGNCC	5 cut(s)	145, 146, 1812, 1970, 2160
ClaI	ATCGAT	1 cut(s)	1005
CseI	GACGC	1 cut(s)	1916
Csp6I	GTAC	8 cut(s)	352, 552, 731, 1213, 1725, 1964, 2058, 2605
CspAI	ACCGGT	1 cut(s)	1215
CviQI	GTAC	8 cut(s)	352, 552, 731, 1213, 1725, 1964, 2058, 2605
DdeI	CTNAG	8 cut(s)	50, 450, 548, 1179, 1491, 1806, 1842, 2591
DinI	GGCGCC	2 cut(s)	127, 253
EaeI	YGGCCR	3 cut(s)	483, 561, 1674
Eam1104I	CTCTTC	5 cut(s)	495, 894, 1927, 2121, 2571
EarI	CTCTTC	5 cut(s)	495, 894, 1927, 2121, 2571
EciI	GGCGGA	2 cut(s)	20, 79
Ecl136II	GAGCTC	2 cut(s)	442, 2379
Eco105I	TACGTA	1 cut(s)	1724
Eco130I	CCWWGG	2 cut(s)	324, 1893
Eco24I	GRGCYC	3 cut(s)	149, 444, 2381
Eco31I	GGTCTC	2 cut(s)	126, 962
Eco47I	GGWCC	2 cut(s)	1812, 2160
Eco53kI	GAGCTC	2 cut(s)	442, 2379
Eco57I	CTGAAG	4 cut(s)	789, 875, 1038, 2595
Eco81I	CCTNAGG	1 cut(s)	1491
Eco88I	CYCGRG	2 cut(s)	191, 227
EcoICRI	GAGCTC	2 cut(s)	442, 2379
EcoRI	GAATTC	1 cut(s)	2241
EcoRII	CCWGG	5 cut(s)	354, 678, 997, 1944, 1966
EcoT14I	CCWWGG	2 cut(s)	324, 1893
EcoT22I	ATGCAT	2 cut(s)	778, 784
EcoT38I	GRGCYC	3 cut(s)	149, 444, 2381
EgeI	GGCGCC	2 cut(s)	127, 253
EheI	GGCGCC	2 cut(s)	127, 253
ErhI	CCWWGG	2 cut(s)	324, 1893
Esp3I	CGTCTC	2 cut(s)	163, 561
FalI	AAGNNNNNCTT	4 cut(s)	1632, 1664, 2455, 2487
FaqI	GGGAC	3 cut(s)	1352, 1411, 1939
FauI	CCCGC	2 cut(s)	156, 717
FauNDI	CATATG	1 cut(s)	778
Fnu4HI	GCNGC	7 cut(s)	102, 181, 269, 2124, 2178, 2181, 2546
FokI	GGATG	7 cut(s)	50, 602, 632, 937, 1508, 2314, 2458
FriOI	GRGCYC	3 cut(s)	149, 444, 2381
Fsp4HI	GCNGC	7 cut(s)	102, 181, 269, 2124, 2178, 2181, 2546
FspBI	CTAG	3 cut(s)	32, 1046, 2567
FspI	TGCGCA	1 cut(s)	2408
GlaI	GCGC	6 cut(s)	100, 127, 223, 225, 253, 2408
GluI	GCNGC	7 cut(s)	102, 181, 269, 2124, 2178, 2181, 2546
GsaI	CCCAGC	2 cut(s)	1546, 1877
HaeII	RGCGCY	2 cut(s)	129, 255
HaeIII	GGCC	7 cut(s)	147, 189, 485, 563, 1676, 1936, 1972
HapII	CCGG	7 cut(s)	129, 437, 564, 954, 1216, 1285, 1422
HgaI	GACGC	1 cut(s)	1916
HhaI	GCGC	6 cut(s)	101, 128, 224, 226, 254, 2409
Hin1I	GRCGYC	3 cut(s)	126, 252, 429
Hin6I	GCGC	6 cut(s)	99, 126, 222, 224, 252, 2407
HinP1I	GCGC	6 cut(s)	99, 126, 222, 224, 252, 2407
HincII	GTYRAC	3 cut(s)	712, 1603, 1978
HindII	GTYRAC	3 cut(s)	712, 1603, 1978
HindIII	AAGCTT	3 cut(s)	1181, 1521, 1682
HpaII	CCGG	7 cut(s)	129, 437, 564, 954, 1216, 1285, 1422
HphI	GGTGA	6 cut(s)	842, 935, 957, 1337, 1880, 2372
Hpy166II	GTNNAC	7 cut(s)	169, 650, 712, 1603, 1637, 1978, 2286
Hpy8I	GTNNAC	7 cut(s)	169, 650, 712, 1603, 1637, 1978, 2286
Hpy99I	CGWCG	3 cut(s)	80, 290, 293
HpyCH4III	ACNGT	5 cut(s)	1152, 1212, 1664, 2062, 2422
HpyCH4IV	ACGT	4 cut(s)	165, 291, 429, 1723
HpyF10VI	GCNNNNNNNGC	5 cut(s)	98, 180, 186, 911, 1838
HpyF3I	CTNAG	8 cut(s)	50, 450, 548, 1179, 1491, 1806, 1842, 2591
HpySE526I	ACGT	4 cut(s)	165, 291, 429, 1723
Hsp92I	GRCGYC	3 cut(s)	126, 252, 429
HspAI	GCGC	6 cut(s)	99, 126, 222, 224, 252, 2407
KasI	GGCGCC	2 cut(s)	125, 251
Kpn2I	TCCGGA	2 cut(s)	1284, 1421
LmnI	GCTCC	4 cut(s)	439, 1492, 1546, 2116
Lsp1109I	GCAGC	5 cut(s)	192, 2135, 2167, 2189, 2532
LweI	GCATC	7 cut(s)	363, 763, 791, 956, 1264, 1849, 2436
MaeI	CTAG	3 cut(s)	32, 1046, 2567
MaeII	ACGT	4 cut(s)	165, 291, 429, 1723
MaeIII	GTNAC	2 cut(s)	963, 1378
MflI	RGATCY	3 cut(s)	1294, 2170, 2347
MhlI	GDGCHC	3 cut(s)	149, 444, 2381
MlsI	TGGCCA	1 cut(s)	485
MluNI	TGGCCA	1 cut(s)	485
Mly113I	GGCGCC	2 cut(s)	126, 252
MlyI	GAGTC	5 cut(s)	203, 754, 866, 1199, 1961
MmeI	TCCRAC	4 cut(s)	519, 597, 2049, 2563
Mox20I	TGGCCA	1 cut(s)	485
Mph1103I	ATGCAT	2 cut(s)	778, 784
MroI	TCCGGA	2 cut(s)	1284, 1421
MroXI	GAANNNNTTC	1 cut(s)	1683
MscI	TGGCCA	1 cut(s)	485
MslI	CAYNNNNRTG	3 cut(s)	1260, 1509, 1857
Msp20I	TGGCCA	1 cut(s)	485
MspA1I	CMGCKG	2 cut(s)	2180, 2545
MspCI	CTTAAG	1 cut(s)	803
MspI	CCGG	7 cut(s)	129, 437, 564, 954, 1216, 1285, 1422
MspR9I	CCNGG	6 cut(s)	356, 437, 680, 999, 1946, 1968
MvaI	CCWGG	5 cut(s)	356, 680, 999, 1946, 1968
MvnI	CGCG	2 cut(s)	207, 224
MwoI	GCNNNNNNNGC	5 cut(s)	98, 180, 186, 911, 1838
NarI	GGCGCC	2 cut(s)	126, 252
NciI	CCSGG	1 cut(s)	437
NcoI	CCATGG	1 cut(s)	1893
NdeI	CATATG	1 cut(s)	778
NlaIV	GGNNCC	7 cut(s)	127, 147, 253, 1234, 1548, 1589, 2193
NmeAIII	GCCGAG	1 cut(s)	198
NmuCI	GTSAC	2 cut(s)	963, 1378
NsbI	TGCGCA	1 cut(s)	2408
NsiI	ATGCAT	2 cut(s)	778, 784
NspI	RCATGY	3 cut(s)	905, 1768, 2605
PaeI	GCATGC	2 cut(s)	905, 1768
PaeR7I	CTCGAG	2 cut(s)	191, 227
PagI	TCATGA	1 cut(s)	817
PaqCI	CACCTGC	2 cut(s)	1220, 1292
PasI	CCCWGGG	1 cut(s)	679
PauI	GCGCGC	1 cut(s)	222
PcsI	WCGNNNNNNNCGW	5 cut(s)	75, 162, 198, 225, 282
PdmI	GAANNNNTTC	1 cut(s)	1683
PfeI	GAWTC	8 cut(s)	8, 419, 475, 926, 1002, 1712, 2050, 2252
PflMI	CCANNNNNTGG	1 cut(s)	264
PfoI	TCCNGGA	2 cut(s)	997, 1944
PinAI	ACCGGT	1 cut(s)	1215
PkrI	GCNGC	7 cut(s)	103, 182, 270, 2125, 2179, 2182, 2547
Ple19I	CGATCG	1 cut(s)	300
PleI	GAGTC	5 cut(s)	202, 753, 865, 1198, 1960
PluTI	GGCGCC	2 cut(s)	129, 255
PpsI	GAGTC	5 cut(s)	202, 753, 865, 1198, 1960
Ppu21I	YACGTR	1 cut(s)	1724
Psp124BI	GAGCTC	2 cut(s)	444, 2381
Psp6I	CCWGG	5 cut(s)	354, 678, 997, 1944, 1966
PspFI	CCCAGC	2 cut(s)	1542, 1873
PspGI	CCWGG	5 cut(s)	354, 678, 997, 1944, 1966
PspN4I	GGNNCC	7 cut(s)	127, 147, 253, 1234, 1548, 1589, 2193
PspOMI	GGGCCC	1 cut(s)	145
PspPI	GGNCC	5 cut(s)	145, 146, 1812, 1970, 2160
PspXI	VCTCGAGB	1 cut(s)	191
PstI	CTGCAG	3 cut(s)	182, 1231, 2185
PsuI	RGATCY	3 cut(s)	1294, 2170, 2347
PteI	GCGCGC	1 cut(s)	222
PvuI	CGATCG	1 cut(s)	300
PvuII	CAGCTG	2 cut(s)	2180, 2545
RsaI	GTAC	8 cut(s)	353, 553, 732, 1214, 1726, 1965, 2059, 2606
RsaNI	GTAC	8 cut(s)	352, 552, 731, 1213, 1725, 1964, 2058, 2605
RseI	CAYNNNNRTG	3 cut(s)	1260, 1509, 1857
SacI	GAGCTC	2 cut(s)	444, 2381
SatI	GCNGC	7 cut(s)	102, 181, 269, 2124, 2178, 2181, 2546
Sau96I	GGNCC	5 cut(s)	145, 146, 1812, 1970, 2160
ScaI	AGTACT	1 cut(s)	553
SchI	GAGTC	5 cut(s)	203, 754, 866, 1199, 1961
ScrFI	CCNGG	6 cut(s)	356, 437, 680, 999, 1946, 1968
SduI	GDGCHC	3 cut(s)	149, 444, 2381
SfaNI	GCATC	7 cut(s)	363, 763, 791, 956, 1264, 1849, 2436
SfcI	CTRYAG	7 cut(s)	178, 1208, 1227, 1557, 2181, 2382, 2391
SfoI	GGCGCC	2 cut(s)	127, 253
Sfr274I	CTCGAG	2 cut(s)	191, 227
SinI	GGWCC	2 cut(s)	1812, 2160
SlaI	CTCGAG	2 cut(s)	191, 227
SmiMI	CAYNNNNRTG	3 cut(s)	1260, 1509, 1857
SmlI	CTYRAG	6 cut(s)	191, 227, 803, 959, 1739, 2495
SmoI	CTYRAG	6 cut(s)	191, 227, 803, 959, 1739, 2495
SnaBI	TACGTA	1 cut(s)	1724
SphI	GCATGC	2 cut(s)	905, 1768
SsiI	CCGC	9 cut(s)	5, 90, 102, 149, 172, 205, 268, 724, 1866
SspDI	GGCGCC	2 cut(s)	125, 251
SspI	AATATT	1 cut(s)	1825
SspMI	CTAG	3 cut(s)	32, 1046, 2567
SstI	GAGCTC	2 cut(s)	444, 2381
StyD4I	CCNGG	6 cut(s)	354, 435, 678, 997, 1944, 1966
StyI	CCWWGG	2 cut(s)	324, 1893
TaaI	ACNGT	5 cut(s)	1152, 1212, 1664, 2062, 2422
TaiI	ACGT	4 cut(s)	168, 294, 432, 1726
TatI	WGTACW	3 cut(s)	551, 730, 2604
TauI	GCSGC	2 cut(s)	104, 271
TfiI	GAWTC	8 cut(s)	8, 419, 475, 926, 1002, 1712, 2050, 2252
TscAI	CASTG	9 cut(s)	345, 471, 1157, 1171, 1231, 1435, 2275, 2317, 2329
TseFI	GTSAC	2 cut(s)	963, 1378
TseI	GCWGC	5 cut(s)	180, 2123, 2177, 2180, 2545
Tsp45I	GTSAC	2 cut(s)	963, 1378
TspGWI	ACGGA	1 cut(s)	130
TspRI	CASTG	9 cut(s)	345, 471, 1157, 1171, 1231, 1435, 2275, 2317, 2329
Van91I	CCANNNNNTGG	1 cut(s)	264
Vha464I	CTTAAG	1 cut(s)	803
VpaK11BI	GGWCC	2 cut(s)	1812, 2160
XapI	RAATTY	6 cut(s)	260, 500, 1408, 1927, 2241, 2432
XceI	RCATGY	3 cut(s)	905, 1768, 2605
XhoI	CTCGAG	2 cut(s)	191, 227
XmnI	GAANNNNTTC	1 cut(s)	1683
XspI	CTAG	3 cut(s)	32, 1046, 2567
ZraI	GACGTC	1 cut(s)	430
ZrmI	AGTACT	1 cut(s)	553
Zsp2I	ATGCAT	2 cut(s)	778, 784

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.