Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0000801

Physical Location & Seq

Genomic Coordinates Forward (+)

58635486 .. 58641372

Gene Structure 5887 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G031480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1863 bp

ATGCATATGCATCTGCTATCTTTTTGTTTCTTAAGGACAAAGTTCATGACCTTGCTGGTGATGATTATCGGATTTCGGTTTTGGAGTCTATTAAGAGGTGGATACAGGTGTATTGTGCAGGCTGTGCCTCTTCAATTCTTGCATGCTCTACTTGCTTATCTTGGTGATTCTGTTAGTTATGAAAGTGCATCATCAGGTCTCAAGTCACCTTTGGCTAAATGCCCTGCTTCATTCTATCCTGGAATCGATACTCCATCGGAAGGTCTTGTAAGATGGCAGTTGCGACTAGAGTATTTTGCTTATGAAACATTGCAAGATTTGAGAATAGCAAAATTATTTGAGATTATTGTGGATTATCCTGACAGCTCTCCTGCGATTGAAGATTTGAAACAGTGTCTTGAATACACTGGACAACATTCTAAGCTTGTGGAGTCATTTATTTCAGCACTACAGTACCGGTTGCTCACTGCAGGTGCCTCAACAAATGACATTTTGCATCAATATGTTTCAACGATCAAGGCACTCCGGACAATAGATCCAGCAGGTGTTTTCCTTGAAGCAGTCGGTGAGCCAATAAGGGACTATTTAAGGGGAAGGAAAGACACCATAAAGTGCATTGTGACCATGCTTACCGATGGGACTGGTGCAAATTCTAATGTATCCGGAAACACTGGCGATAGCCTTCTTGAAGAATTAAATAGAGATGAAGAAAGCCAAGAGAACATTGCTCCTGAGGATGACTTTCATACTGATGATAAGCAAGCTTGGATAAATGCTTCACGCTGGGAGCCTGACCCTGTAGAAGCTGACCCCTTGAAAGGTAGCAGGAACCGAAGGAAGGTTGACATACTTGGAATGATTGTTGGCATAATTGGTTCACAAGACCAACTTGTTAATGAATACCGTGTTATGCTGGCCGAAAAGCTTCTCAACAAATCTGATTATGACATTGATTCAGAGATACGTACTCTTGAACTCCTCAAGATACATTTTGGAGAGAACAGCATGCAGAAATGTGAAATTATGCTCAATGATCTGATTGGTTCTAAGAGGACCAACACTAATATTAAAGCAACCATAGCTCAGCCATCTCATACAGATGCAGGCGGTGAGCTGGGGGATAACAGGGTATCCATGGATATTCTTGATGCTACAATTATCTCTTCAAATTTTTGGCCTTCAATCCAGGAGGAGTCCCTAAATGTACCTGGGCCTGTTGACCAACTACTCTCTGATTATGCAAAGAGATTTAATGAAATCAAGACCCCTCGTAAGCTATTGTGGAAGAAGAATCTTGGTACTGTCAAGTTGGAATTGCAATTTGAAGATAGAGAAATGCAGTTCATGGTTGCTCCTCTTCATGCAGCAATCGTAATGCAATTTCAAGATCAGAAAAGCTGGACCTCCAAAGATCTTGCAGCTGCAGTTGGGGTTCCAATAGATATACTTAATCGGAGGATCAATTTTTGGATAAGCAAGGGAATTCTTGCAGAATCACTTGGGGAAGACGCTGATGACCATGTGTTTACACTAATGGAAGGGATGATTGACACTGGCAAAAACACTGCTAATGATGGGAACTGCGAGGATCTTATAGTTGGTGATGAGGAAGGAGAGAACTCTGTAGCCTCTGTAGAGGATCAACTGCGCAAGGAAATGACTGTGTATGAGAAATTTATTTTGGGGATGCTCACAAACTTTGGAAGCATGGCTTTGGATCGTATTCATAATACTCTCAAGATGTTCTGCATAGCTGATCCTACTTATGACAAAACCCTCCAACAGCTGCAAAGTTTCTTATCAGGTCTAGTTTCTGAAGAGAAATTAGAACTAAGAGATGGCATGTACTTTTTGAAGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000152 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005680 GO:0005819 GO:0005856 GO:0007275 GO:0008150 GO:0009553 GO:0009561 GO:0015630 GO:0019899 GO:0031461 GO:0031625 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0048229 GO:0048856 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166

Pfam Domains

Protein Families

ANAPC2 Cullin

Protein Analysis

620

Amino Acids

69.85

Weight (kDa)

4.92

Isoelectric Point (pI)

38.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_ANAPC2	PF25773	91 - 199	5.7e-42	ANAPC2-like, TPR repeats
Cullin_AB	PF26557	386 - 498	4.9e-15	Cullin alpha+beta domain
ANAPC2	PF08672	559 - 616	1.3e-23	Anaphase promoting complex (APC) subunit 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000801)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G04660
fragaria_vesca	FvH4_5g29350 FvH4_5g29350 FvH4_5g29350 FvH4_5g29350 FvH4_5g29350
malus_domestica	MD13G1068900.v1.1 MD16G1069200.v1.1
prunus_persica	Prupe.1G035400_v2.0.a1 Prupe.1G035400_v2.0.a1 Prupe.1G191200_v2.0.a1
pyrus_communis	pycom16g06020
rosa_chinensis	RchiOBHm_Chr1g0327871 RchiOBHm_Chr2g0121271 RchiOBHm_Chr4g0439421 RchiOBHm_Chr7g0200631
rosa_laevigata	RLG00000006274 RLG00000011822 RLG00000018454 RLG00000034065 RLG00000034779 RLG00000035517
rosa_multiflora	Rmu_sc0002635.1_g000001 Rmu_sc0006782.1_g000010 Rmu_sc0037339.1_g000001
rosa_roxburghii	Rroxscaffold_159G00433030 Rroxscaffold_3G00237290 Rroxscaffold_3G00259010 Rroxscaffold_4G00283810 Rroxscaffold_5G00380330 Rroxscaffold_6G00397040 Rroxscaffold_7G00199350 Rroxscaffold_7G00199460 Rroxscaffold_7G00212760 Rroxscaffold_7G00213330
rosa_rugosa	Rorug01G0198100 Rorug03G0008800 Rorug04G0030200 Rorug04G0317800 Rorug04G0317900 Rorug04G0318000 Rorug04G0443500 Rorug05G0260700 Rorug05G0260800 Rorug05G0536400 Rorug05G0536500
rosa_samantha	Rh1BG117800 Rh2AG412900 Rh2BG241400 Rh2CG278400 Rh2CG398900 Rh2CG490800 Rh4AG369300 Rh4BG017200 Rh4BG381100 Rh4CG395700 Rh4DG375700 Rh4DG375800 Rh5AG534500 Rh5BG490100 Rh5CG514100 Rh5DG386800 Rh6BG137000 Rh6CG037000 Rh6DG121500 Rh7CG205400
rosa_wichuraiana	Rw3G022720 Rw4G031480 Rw6G043670

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	461, 533
Acc16I	TGCGCA	1 cut(s)	1649
Acc36I	ACCTGC	2 cut(s)	461, 533
AccB1I	GGYRCC	1 cut(s)	473
AccIII	TCCGGA	2 cut(s)	525, 662
AciI	CCGC	1 cut(s)	1107
AclWI	GGATC	6 cut(s)	530, 1466, 1596, 1647, 1725, 1751
AcoI	YGGCCR	1 cut(s)	915
AcsI	RAATTY	4 cut(s)	649, 1168, 1482, 1673
AcuI	CTGAAG	1 cut(s)	1836
AfaI	GTAC	5 cut(s)	455, 967, 1206, 1300, 1847
AfiI	CCNNNNNNNGG	3 cut(s)	260, 818, 838
AflII	CTTAAG	1 cut(s)	31
AgeI	ACCGGT	1 cut(s)	456
AjnI	CCWGG	3 cut(s)	238, 1185, 1207
AjuI	GAANNNNNNNTTGG	2 cut(s)	1664, 1696
Alw26I	GTCTC	1 cut(s)	203
AlwI	GGATC	6 cut(s)	530, 1466, 1596, 1647, 1725, 1751
Aor13HI	TCCGGA	2 cut(s)	525, 662
AoxI	GGCC	3 cut(s)	915, 1175, 1211
ApeKI	GCWGC	4 cut(s)	1364, 1418, 1421, 1786
ApoI	RAATTY	4 cut(s)	649, 1168, 1482, 1673
AsiGI	ACCGGT	1 cut(s)	456
Asp700I	GAANNNNTTC	1 cut(s)	924
AspLEI	GCGC	1 cut(s)	1650
AspS9I	GGNCC	3 cut(s)	1053, 1211, 1401
AsuHPI	GGTGA	6 cut(s)	70, 176, 198, 578, 1121, 1613
AvaII	GGWCC	2 cut(s)	1053, 1401
AxyI	CCTNAGG	1 cut(s)	732
BaeI	ACNNNNGTAYC	4 cut(s)	94, 127, 437, 470
BanI	GGYRCC	1 cut(s)	473
BbsI	GAAGAC	1 cut(s)	1512
BbvI	GCAGC	4 cut(s)	1376, 1408, 1430, 1773
BccI	CCATC	6 cut(s)	262, 267, 629, 1096, 1568, 1832
BciT130I	CCWGG	3 cut(s)	240, 1187, 1209
BciVI	GTATCC	3 cut(s)	95, 670, 1141
BcoDI	GTCTC	1 cut(s)	203
BfaI	CTAG	2 cut(s)	287, 1808
BfmI	CTRYAG	6 cut(s)	449, 468, 798, 1422, 1623, 1632
BfrI	CTTAAG	1 cut(s)	31
BfuAI	ACCTGC	2 cut(s)	461, 533
BfuI	GTATCC	3 cut(s)	95, 670, 1141
BglII	AGATCT	1 cut(s)	1411
BisI	GCNGC	4 cut(s)	1365, 1419, 1422, 1787
BlpI	GCTNAGC	1 cut(s)	1083
BlsI	GCNGC	4 cut(s)	1366, 1420, 1423, 1788
Bme1390I	CCNGG	3 cut(s)	240, 1187, 1209
Bme18I	GGWCC	2 cut(s)	1053, 1401
BmgT120I	GGNCC	3 cut(s)	1053, 1211, 1401
BmiI	GGNNCC	4 cut(s)	475, 789, 830, 1434
BmrFI	CCNGG	3 cut(s)	240, 1187, 1209
BmsI	GCATC	6 cut(s)	19, 197, 505, 1090, 1138, 1677
BpiI	GAAGAC	1 cut(s)	1512
Bpu1102I	GCTNAGC	1 cut(s)	1083
BpuEI	CTTGAG	3 cut(s)	185, 965, 1721
Bsa29I	ATCGAT	1 cut(s)	246
BsaAI	YACGTR	1 cut(s)	965
BsaBI	GATNNNNATC	1 cut(s)	65
BsaI	GGTCTC	1 cut(s)	203
BsaJI	CCNNGG	2 cut(s)	1134, 1208
BsaWI	WCCGGW	3 cut(s)	456, 525, 662
Bsc4I	CCNNNNNNNGG	3 cut(s)	260, 818, 838
Bse118I	RCCGGY	1 cut(s)	456
Bse1I	ACTGG	4 cut(s)	412, 646, 676, 1558
Bse21I	CCTNAGG	1 cut(s)	732
Bse3DI	GCAATG	2 cut(s)	308, 723
Bse8I	GATNNNNATC	1 cut(s)	65
BseAI	TCCGGA	2 cut(s)	525, 662
BseBI	CCWGG	3 cut(s)	240, 1187, 1209
BseCI	ATCGAT	1 cut(s)	246
BseDI	CCNNGG	2 cut(s)	1134, 1208
BseGI	GGATG	3 cut(s)	742, 1548, 1692
BseJI	GATNNNNATC	1 cut(s)	65
BseLI	CCNNNNNNNGG	3 cut(s)	260, 818, 838
BseMI	GCAATG	2 cut(s)	308, 723
BseMII	CTCAG	2 cut(s)	723, 1097
BseNI	ACTGG	4 cut(s)	412, 646, 676, 1558
BseRI	GAGGAG	3 cut(s)	968, 1205, 1344
BseXI	GCAGC	4 cut(s)	1376, 1408, 1430, 1773
BseYI	CCCAGC	2 cut(s)	783, 1114
BsgI	GTGCAG	1 cut(s)	137
BshFI	GGCC	3 cut(s)	917, 1177, 1213
BshNI	GGYRCC	1 cut(s)	473
BshTI	ACCGGT	1 cut(s)	456
BshVI	ATCGAT	1 cut(s)	246
BsiSI	CCGG	3 cut(s)	457, 526, 663
BslFI	GGGAC	3 cut(s)	593, 652, 1180
BslI	CCNNNNNNNGG	3 cut(s)	260, 818, 838
BsmAI	GTCTC	1 cut(s)	203
BsmFI	GGGAC	3 cut(s)	593, 652, 1180
BsnI	GGCC	3 cut(s)	917, 1177, 1213
Bso31I	GGTCTC	1 cut(s)	203
Bsp13I	TCCGGA	2 cut(s)	525, 662
Bsp1720I	GCTNAGC	1 cut(s)	1083
Bsp19I	CCATGG	1 cut(s)	1134
BspACI	CCGC	1 cut(s)	1107
BspANI	GGCC	3 cut(s)	917, 1177, 1213
BspCNI	CTCAG	2 cut(s)	724, 1096
BspDI	ATCGAT	1 cut(s)	246
BspEI	TCCGGA	2 cut(s)	525, 662
BspHI	TCATGA	1 cut(s)	45
BspLI	GGNNCC	4 cut(s)	475, 789, 830, 1434
BspMAI	CTGCAG	2 cut(s)	472, 1426
BspMI	ACCTGC	2 cut(s)	461, 533
BspPI	GGATC	6 cut(s)	530, 1466, 1596, 1647, 1725, 1751
BspT107I	GGYRCC	1 cut(s)	473
BspTI	CTTAAG	1 cut(s)	31
BspTNI	GGTCTC	1 cut(s)	203
BsrDI	GCAATG	2 cut(s)	308, 723
BsrFI	RCCGGY	1 cut(s)	456
BsrI	ACTGG	4 cut(s)	412, 646, 676, 1558
BssAI	RCCGGY	1 cut(s)	456
BssECI	CCNNGG	2 cut(s)	1134, 1208
BssT1I	CCWWGG	1 cut(s)	1134
Bst2UI	CCWGG	3 cut(s)	240, 1187, 1209
Bst4CI	ACNGT	5 cut(s)	393, 453, 905, 1303, 1663
Bst6I	CTCTTC	4 cut(s)	135, 1168, 1362, 1812
BstAFI	CTTAAG	1 cut(s)	31
BstAPI	GCANNNNNTGC	1 cut(s)	124
BstBAI	YACGTR	1 cut(s)	965
BstC8I	GCNNGC	6 cut(s)	120, 144, 762, 915, 1007, 1105
BstDEI	CTNAG	5 cut(s)	420, 732, 1047, 1083, 1832
BstDSI	CCRYGG	1 cut(s)	1134
BstF5I	GGATG	3 cut(s)	742, 1548, 1692
BstHHI	GCGC	1 cut(s)	1650
BstMAI	GTCTC	1 cut(s)	203
BstMWI	GCNNNNNNNGC	3 cut(s)	124, 152, 1079
BstNI	CCWGG	3 cut(s)	240, 1187, 1209
BstNSI	RCATGY	3 cut(s)	146, 1009, 1846
BstSCI	CCNGG	3 cut(s)	238, 1185, 1207
BstSFI	CTRYAG	6 cut(s)	449, 468, 798, 1422, 1623, 1632
BstSNI	TACGTA	1 cut(s)	965
BstV1I	GCAGC	4 cut(s)	1376, 1408, 1430, 1773
BstV2I	GAAGAC	1 cut(s)	1512
BstX2I	RGATCY	3 cut(s)	535, 1411, 1588
BstYI	RGATCY	3 cut(s)	535, 1411, 1588
Bsu15I	ATCGAT	1 cut(s)	246
Bsu36I	CCTNAGG	1 cut(s)	732
BsuI	GTATCC	3 cut(s)	95, 670, 1141
BsuRI	GGCC	3 cut(s)	917, 1177, 1213
BsuTUI	ATCGAT	1 cut(s)	246
BtgI	CCRYGG	1 cut(s)	1134
BtsCI	GGATG	3 cut(s)	742, 1548, 1692
BtsI	GCAGTG	2 cut(s)	465, 1563
BtsIMutI	CAGTG	6 cut(s)	398, 405, 465, 669, 1551, 1563
BveI	ACCTGC	2 cut(s)	461, 533
Cac8I	GCNNGC	6 cut(s)	120, 144, 762, 915, 1007, 1105
CciI	TCATGA	1 cut(s)	45
CfoI	GCGC	1 cut(s)	1650
Cfr10I	RCCGGY	1 cut(s)	456
Cfr13I	GGNCC	3 cut(s)	1053, 1211, 1401
ClaI	ATCGAT	1 cut(s)	246
CseI	GACGC	1 cut(s)	1517
Csp6I	GTAC	5 cut(s)	454, 966, 1205, 1299, 1846
CspAI	ACCGGT	1 cut(s)	456
CviQI	GTAC	5 cut(s)	454, 966, 1205, 1299, 1846
DdeI	CTNAG	5 cut(s)	420, 732, 1047, 1083, 1832
EaeI	YGGCCR	1 cut(s)	915
Eam1104I	CTCTTC	4 cut(s)	135, 1168, 1362, 1812
EarI	CTCTTC	4 cut(s)	135, 1168, 1362, 1812
Eco105I	TACGTA	1 cut(s)	965
Eco130I	CCWWGG	1 cut(s)	1134
Eco31I	GGTCTC	1 cut(s)	203
Eco47I	GGWCC	2 cut(s)	1053, 1401
Eco57I	CTGAAG	1 cut(s)	1836
Eco81I	CCTNAGG	1 cut(s)	732
EcoRI	GAATTC	1 cut(s)	1482
EcoRII	CCWGG	3 cut(s)	238, 1185, 1207
EcoT14I	CCWWGG	1 cut(s)	1134
EcoT22I	ATGCAT	2 cut(s)	6, 12
ErhI	CCWWGG	1 cut(s)	1134
FalI	AAGNNNNNCTT	4 cut(s)	873, 905, 1696, 1728
FaqI	GGGAC	3 cut(s)	593, 652, 1180
FauNDI	CATATG	1 cut(s)	6
Fnu4HI	GCNGC	4 cut(s)	1365, 1419, 1422, 1787
FokI	GGATG	3 cut(s)	749, 1555, 1699
Fsp4HI	GCNGC	4 cut(s)	1365, 1419, 1422, 1787
FspBI	CTAG	2 cut(s)	287, 1808
FspI	TGCGCA	1 cut(s)	1649
GlaI	GCGC	1 cut(s)	1649
GluI	GCNGC	4 cut(s)	1365, 1419, 1422, 1787
GsaI	CCCAGC	2 cut(s)	787, 1118
HaeIII	GGCC	3 cut(s)	917, 1177, 1213
HapII	CCGG	3 cut(s)	457, 526, 663
HgaI	GACGC	1 cut(s)	1517
HhaI	GCGC	1 cut(s)	1650
Hin6I	GCGC	1 cut(s)	1648
HinP1I	GCGC	1 cut(s)	1648
HincII	GTYRAC	2 cut(s)	844, 1219
HindII	GTYRAC	2 cut(s)	844, 1219
HindIII	AAGCTT	3 cut(s)	422, 762, 923
HinfI	GANTC	8 cut(s)	85, 167, 243, 431, 953, 1193, 1291, 1493
HpaII	CCGG	3 cut(s)	457, 526, 663
HphI	GGTGA	6 cut(s)	70, 176, 198, 578, 1121, 1613
Hpy166II	GTNNAC	4 cut(s)	844, 878, 1219, 1527
Hpy188I	TCNGA	9 cut(s)	71, 259, 940, 958, 1038, 1234, 1392, 1455, 1816
Hpy8I	GTNNAC	4 cut(s)	844, 878, 1219, 1527
HpyAV	CCTTC	8 cut(s)	254, 588, 692, 828, 832, 1188, 1532, 1604
HpyCH4III	ACNGT	5 cut(s)	393, 453, 905, 1303, 1663
HpyCH4IV	ACGT	1 cut(s)	964
HpyF10VI	GCNNNNNNNGC	3 cut(s)	124, 152, 1079
HpyF3I	CTNAG	5 cut(s)	420, 732, 1047, 1083, 1832
HpySE526I	ACGT	1 cut(s)	964
HspAI	GCGC	1 cut(s)	1648
Kpn2I	TCCGGA	2 cut(s)	525, 662
LmnI	GCTCC	3 cut(s)	733, 787, 1357
Lsp1109I	GCAGC	4 cut(s)	1376, 1408, 1430, 1773
LweI	GCATC	6 cut(s)	19, 197, 505, 1090, 1138, 1677
MaeI	CTAG	2 cut(s)	287, 1808
MaeII	ACGT	1 cut(s)	964
MaeIII	GTNAC	2 cut(s)	204, 619
MflI	RGATCY	3 cut(s)	535, 1411, 1588
MlyI	GAGTC	3 cut(s)	94, 440, 1202
MmeI	TCCRAC	2 cut(s)	1290, 1804
Mph1103I	ATGCAT	2 cut(s)	6, 12
MroI	TCCGGA	2 cut(s)	525, 662
MroXI	GAANNNNTTC	1 cut(s)	924
MseI	TTAA	8 cut(s)	32, 92, 587, 695, 894, 1068, 1251, 1449
MslI	CAYNNNNRTG	3 cut(s)	501, 750, 1098
MspA1I	CMGCKG	2 cut(s)	1421, 1786
MspCI	CTTAAG	1 cut(s)	31
MspI	CCGG	3 cut(s)	457, 526, 663
MspR9I	CCNGG	3 cut(s)	240, 1187, 1209
MvaI	CCWGG	3 cut(s)	240, 1187, 1209
MwoI	GCNNNNNNNGC	3 cut(s)	124, 152, 1079
NcoI	CCATGG	1 cut(s)	1134
NdeI	CATATG	1 cut(s)	6
NlaIV	GGNNCC	4 cut(s)	475, 789, 830, 1434
NmuCI	GTSAC	2 cut(s)	204, 619
NsbI	TGCGCA	1 cut(s)	1649
NsiI	ATGCAT	2 cut(s)	6, 12
NspI	RCATGY	3 cut(s)	146, 1009, 1846
PaeI	GCATGC	2 cut(s)	146, 1009
PagI	TCATGA	1 cut(s)	45
PaqCI	CACCTGC	2 cut(s)	461, 533
PdmI	GAANNNNTTC	1 cut(s)	924
PfeI	GAWTC	5 cut(s)	167, 243, 953, 1291, 1493
PfoI	TCCNGGA	2 cut(s)	238, 1185
PinAI	ACCGGT	1 cut(s)	456
PkrI	GCNGC	4 cut(s)	1366, 1420, 1423, 1788
PleI	GAGTC	3 cut(s)	93, 439, 1201
PpsI	GAGTC	3 cut(s)	93, 439, 1201
Ppu21I	YACGTR	1 cut(s)	965
Psp6I	CCWGG	3 cut(s)	238, 1185, 1207
PspFI	CCCAGC	2 cut(s)	783, 1114
PspGI	CCWGG	3 cut(s)	238, 1185, 1207
PspN4I	GGNNCC	4 cut(s)	475, 789, 830, 1434
PspPI	GGNCC	3 cut(s)	1053, 1211, 1401
PstI	CTGCAG	2 cut(s)	472, 1426
PsuI	RGATCY	3 cut(s)	535, 1411, 1588
PvuII	CAGCTG	2 cut(s)	1421, 1786
RsaI	GTAC	5 cut(s)	455, 967, 1206, 1300, 1847
RsaNI	GTAC	5 cut(s)	454, 966, 1205, 1299, 1846
RseI	CAYNNNNRTG	3 cut(s)	501, 750, 1098
SaqAI	TTAA	8 cut(s)	32, 92, 587, 695, 894, 1068, 1251, 1449
SatI	GCNGC	4 cut(s)	1365, 1419, 1422, 1787
Sau96I	GGNCC	3 cut(s)	1053, 1211, 1401
SchI	GAGTC	3 cut(s)	94, 440, 1202
ScrFI	CCNGG	3 cut(s)	240, 1187, 1209
SfaNI	GCATC	6 cut(s)	19, 197, 505, 1090, 1138, 1677
SfcI	CTRYAG	6 cut(s)	449, 468, 798, 1422, 1623, 1632
SinI	GGWCC	2 cut(s)	1053, 1401
SmiMI	CAYNNNNRTG	3 cut(s)	501, 750, 1098
SmlI	CTYRAG	4 cut(s)	31, 200, 980, 1736
SmoI	CTYRAG	4 cut(s)	31, 200, 980, 1736
SnaBI	TACGTA	1 cut(s)	965
SphI	GCATGC	2 cut(s)	146, 1009
SsiI	CCGC	1 cut(s)	1107
SspI	AATATT	1 cut(s)	1066
SspMI	CTAG	2 cut(s)	287, 1808
StyD4I	CCNGG	3 cut(s)	238, 1185, 1207
StyI	CCWWGG	1 cut(s)	1134
TaaI	ACNGT	5 cut(s)	393, 453, 905, 1303, 1663
TaiI	ACGT	1 cut(s)	967
TaqI	TCGA	1 cut(s)	246
TatI	WGTACW	1 cut(s)	1845
TfiI	GAWTC	5 cut(s)	167, 243, 953, 1291, 1493
Tru1I	TTAA	8 cut(s)	32, 92, 587, 695, 894, 1068, 1251, 1449
Tru9I	TTAA	8 cut(s)	32, 92, 587, 695, 894, 1068, 1251, 1449
TscAI	CASTG	6 cut(s)	398, 412, 472, 676, 1558, 1570
TseFI	GTSAC	2 cut(s)	204, 619
TseI	GCWGC	4 cut(s)	1364, 1418, 1421, 1786
Tsp45I	GTSAC	2 cut(s)	204, 619
TspRI	CASTG	6 cut(s)	398, 412, 472, 676, 1558, 1570
Vha464I	CTTAAG	1 cut(s)	31
VpaK11BI	GGWCC	2 cut(s)	1053, 1401
XapI	RAATTY	4 cut(s)	649, 1168, 1482, 1673
XceI	RCATGY	3 cut(s)	146, 1009, 1846
XmnI	GAANNNNTTC	1 cut(s)	924
XspI	CTAG	2 cut(s)	287, 1808
Zsp2I	ATGCAT	2 cut(s)	6, 12

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.