Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1208 |
| Acc16I | TGCGCA | 1 cut(s) | 470 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 901 |
| AciI | CCGC | 4 cut(s) | 178, 279, 1471, 1544 |
| AclI | AACGTT | 1 cut(s) | 1702 |
| AclWI | GGATC | 1 cut(s) | 713 |
| AcsI | RAATTY | 2 cut(s) | 356, 1304 |
| AcuI | CTGAAG | 2 cut(s) | 925, 1515 |
| AfaI | GTAC | 6 cut(s) | 171, 856, 1215, 1275, 1432, 1506 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 901 |
| AflII | CTTAAG | 1 cut(s) | 1175 |
| AflIII | ACRYGT | 1 cut(s) | 342 |
| AgsI | TTSAA | 5 cut(s) | 191, 635, 922, 1031, 1460 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1601 |
| AjnI | CCWGG | 1 cut(s) | 98 |
| Alw26I | GTCTC | 1 cut(s) | 162 |
| AlwI | GGATC | 1 cut(s) | 713 |
| Ama87I | CYCGRG | 2 cut(s) | 1335, 1673 |
| AoxI | GGCC | 2 cut(s) | 250, 903 |
| ApeKI | GCWGC | 8 cut(s) | 33, 69, 226, 351, 794, 818, 1490, 1636 |
| ApoI | RAATTY | 2 cut(s) | 356, 1304 |
| AseI | ATTAAT | 2 cut(s) | 618, 1034 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 215, 1440 |
| AspLEI | GCGC | 2 cut(s) | 471, 849 |
| AspS9I | GGNCC | 2 cut(s) | 286, 1595 |
| AsuC2I | CCSGG | 1 cut(s) | 1166 |
| AsuHPI | GGTGA | 3 cut(s) | 382, 941, 1273 |
| AsuII | TTCGAA | 1 cut(s) | 1328 |
| AvaI | CYCGRG | 2 cut(s) | 1335, 1673 |
| AvaII | GGWCC | 2 cut(s) | 286, 1595 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1488, 1520 |
| BbsI | GAAGAC | 4 cut(s) | 642, 931, 938, 1115 |
| BbvI | GCAGC | 8 cut(s) | 20, 56, 213, 363, 805, 806, 1502, 1648 |
| BccI | CCATC | 2 cut(s) | 276, 1705 |
| BceAI | ACGGC | 2 cut(s) | 474, 1487 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1625, 1659 |
| BciT130I | CCWGG | 1 cut(s) | 100 |
| BcnI | CCSGG | 1 cut(s) | 1166 |
| BcoDI | GTCTC | 1 cut(s) | 162 |
| BfaI | CTAG | 3 cut(s) | 119, 1514, 1547 |
| BfmI | CTRYAG | 3 cut(s) | 254, 966, 1659 |
| BfrI | CTTAAG | 1 cut(s) | 1175 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 249 |
| BglII | AGATCT | 2 cut(s) | 661, 1438 |
| BisI | GCNGC | 8 cut(s) | 34, 70, 227, 352, 795, 819, 1491, 1637 |
| BlsI | GCNGC | 8 cut(s) | 35, 71, 228, 353, 796, 820, 1492, 1638 |
| Bme1390I | CCNGG | 2 cut(s) | 100, 1166 |
| Bme18I | GGWCC | 2 cut(s) | 286, 1595 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1601 |
| BmeT110I | CYCGRG | 2 cut(s) | 1335, 1673 |
| BmgT120I | GGNCC | 2 cut(s) | 286, 1595 |
| BmiI | GGNNCC | 2 cut(s) | 287, 1022 |
| BmrFI | CCNGG | 2 cut(s) | 100, 1166 |
| BmsI | GCATC | 7 cut(s) | 456, 562, 858, 1000, 1183, 1268, 1489 |
| BpiI | GAAGAC | 4 cut(s) | 642, 931, 938, 1115 |
| BpmI | CTGGAG | 1 cut(s) | 1611 |
| Bpu14I | TTCGAA | 1 cut(s) | 1328 |
| BpuEI | CTTGAG | 1 cut(s) | 523 |
| BpuMI | CCSGG | 1 cut(s) | 1166 |
| Bsa29I | ATCGAT | 1 cut(s) | 1425 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 901 |
| BseBI | CCWGG | 1 cut(s) | 100 |
| BseCI | ATCGAT | 1 cut(s) | 1425 |
| BseGI | GGATG | 9 cut(s) | 79, 287, 471, 577, 721, 986, 1015, 1686, 1716 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 901 |
| BseMII | CTCAG | 1 cut(s) | 840 |
| BseRI | GAGGAG | 1 cut(s) | 581 |
| BseXI | GCAGC | 8 cut(s) | 20, 56, 213, 363, 805, 806, 1502, 1648 |
| BshFI | GGCC | 2 cut(s) | 252, 905 |
| BshVI | ATCGAT | 1 cut(s) | 1425 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1335, 1673 |
| BsiSI | CCGG | 1 cut(s) | 1165 |
| BslFI | GGGAC | 5 cut(s) | 137, 194, 389, 1181, 1547 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 901 |
| BsmAI | GTCTC | 1 cut(s) | 162 |
| BsmBI | CGTCTC | 1 cut(s) | 162 |
| BsmFI | GGGAC | 5 cut(s) | 137, 194, 389, 1181, 1547 |
| BsmI | GAATGC | 3 cut(s) | 534, 1673, 1693 |
| BsnI | GGCC | 2 cut(s) | 252, 905 |
| BsoBI | CYCGRG | 2 cut(s) | 1335, 1673 |
| Bsp119I | TTCGAA | 1 cut(s) | 1328 |
| Bsp1407I | TGTACA | 1 cut(s) | 1213 |
| Bsp143I | GATC | 4 cut(s) | 661, 718, 873, 1438 |
| BspACI | CCGC | 4 cut(s) | 178, 279, 1471, 1544 |
| BspANI | GGCC | 2 cut(s) | 252, 905 |
| BspCNI | CTCAG | 1 cut(s) | 839 |
| BspDI | ATCGAT | 1 cut(s) | 1425 |
| BspHI | TCATGA | 2 cut(s) | 870, 1566 |
| BspLI | GGNNCC | 2 cut(s) | 287, 1022 |
| BspMAI | CTGCAG | 2 cut(s) | 970, 1663 |
| BspPI | GGATC | 1 cut(s) | 713 |
| BspT104I | TTCGAA | 1 cut(s) | 1328 |
| BspTI | CTTAAG | 1 cut(s) | 1175 |
| BsrGI | TGTACA | 1 cut(s) | 1213 |
| BssMI | GATC | 4 cut(s) | 661, 718, 873, 1438 |
| Bst2UI | CCWGG | 1 cut(s) | 100 |
| Bst4CI | ACNGT | 6 cut(s) | 169, 238, 314, 544, 1114, 1204 |
| Bst6I | CTCTTC | 2 cut(s) | 393, 507 |
| BstAFI | CTTAAG | 1 cut(s) | 1175 |
| BstAUI | TGTACA | 1 cut(s) | 1213 |
| BstBI | TTCGAA | 1 cut(s) | 1328 |
| BstC8I | GCNNGC | 6 cut(s) | 38, 277, 966, 1074, 1641, 1645 |
| BstDEI | CTNAG | 4 cut(s) | 29, 826, 954, 1103 |
| BstF5I | GGATG | 9 cut(s) | 79, 287, 471, 577, 721, 986, 1015, 1686, 1716 |
| BstHHI | GCGC | 2 cut(s) | 471, 849 |
| BstKTI | GATC | 4 cut(s) | 664, 721, 876, 1441 |
| BstMAI | GTCTC | 1 cut(s) | 162 |
| BstMBI | GATC | 4 cut(s) | 661, 718, 873, 1438 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 146, 249, 827, 846, 1499 |
| BstNI | CCWGG | 1 cut(s) | 100 |
| BstNSI | RCATGY | 2 cut(s) | 346, 392 |
| BstSCI | CCNGG | 2 cut(s) | 98, 1164 |
| BstSFI | CTRYAG | 3 cut(s) | 254, 966, 1659 |
| BstV1I | GCAGC | 8 cut(s) | 20, 56, 213, 363, 805, 806, 1502, 1648 |
| BstV2I | GAAGAC | 4 cut(s) | 642, 931, 938, 1115 |
| BstX2I | RGATCY | 2 cut(s) | 661, 1438 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 712 |
| BstYI | RGATCY | 2 cut(s) | 661, 1438 |
| Bsu15I | ATCGAT | 1 cut(s) | 1425 |
| BsuRI | GGCC | 2 cut(s) | 252, 905 |
| BsuTUI | ATCGAT | 1 cut(s) | 1425 |
| BtsCI | GGATG | 9 cut(s) | 79, 287, 471, 577, 721, 986, 1015, 1686, 1716 |
| BtsIMutI | CAGTG | 2 cut(s) | 1195, 1241 |
| Cac8I | GCNNGC | 6 cut(s) | 38, 277, 966, 1074, 1641, 1645 |
| CciI | TCATGA | 2 cut(s) | 870, 1566 |
| CfoI | GCGC | 2 cut(s) | 471, 849 |
| Cfr13I | GGNCC | 2 cut(s) | 286, 1595 |
| ClaI | ATCGAT | 1 cut(s) | 1425 |
| Csp6I | GTAC | 6 cut(s) | 170, 855, 1214, 1274, 1431, 1505 |
| CviAII | CATG | 9 cut(s) | 145, 343, 389, 426, 483, 871, 1017, 1567, 1632 |
| CviQI | GTAC | 6 cut(s) | 170, 855, 1214, 1274, 1431, 1505 |
| DdeI | CTNAG | 4 cut(s) | 29, 826, 954, 1103 |
| DpnI | GATC | 4 cut(s) | 663, 720, 875, 1440 |
| DpnII | GATC | 4 cut(s) | 661, 718, 873, 1438 |
| DraI | TTTAAA | 1 cut(s) | 1486 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1601 |
| Eam1104I | CTCTTC | 2 cut(s) | 393, 507 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1601 |
| EarI | CTCTTC | 2 cut(s) | 393, 507 |
| EciI | GGCGGA | 2 cut(s) | 294, 1486 |
| Eco32I | GATATC | 2 cut(s) | 328, 1564 |
| Eco47I | GGWCC | 2 cut(s) | 286, 1595 |
| Eco57I | CTGAAG | 2 cut(s) | 925, 1515 |
| Eco88I | CYCGRG | 2 cut(s) | 1335, 1673 |
| EcoRII | CCWGG | 1 cut(s) | 98 |
| EcoRV | GATATC | 2 cut(s) | 328, 1564 |
| EcoT22I | ATGCAT | 1 cut(s) | 577 |
| Esp3I | CGTCTC | 1 cut(s) | 162 |
| FaeI | CATG | 9 cut(s) | 148, 346, 392, 429, 486, 874, 1020, 1570, 1635 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1060, 1092 |
| FaqI | GGGAC | 5 cut(s) | 137, 194, 389, 1181, 1547 |
| FatI | CATG | 9 cut(s) | 144, 342, 388, 425, 482, 870, 1016, 1566, 1631 |
| FauI | CCCGC | 1 cut(s) | 171 |
| FauNDI | CATATG | 1 cut(s) | 473 |
| Fnu4HI | GCNGC | 8 cut(s) | 34, 70, 227, 352, 795, 819, 1491, 1637 |
| FokI | GGATG | 8 cut(s) | 66, 294, 478, 584, 728, 993, 1022, 1693 |
| Fsp4HI | GCNGC | 8 cut(s) | 34, 70, 227, 352, 795, 819, 1491, 1637 |
| FspAI | RTGCGCAY | 1 cut(s) | 470 |
| FspBI | CTAG | 3 cut(s) | 119, 1514, 1547 |
| FspI | TGCGCA | 1 cut(s) | 470 |
| GlaI | GCGC | 2 cut(s) | 470, 848 |
| GluI | GCNGC | 8 cut(s) | 34, 70, 227, 352, 795, 819, 1491, 1637 |
| GsuI | CTGGAG | 1 cut(s) | 1611 |
| HaeIII | GGCC | 2 cut(s) | 252, 905 |
| HapII | CCGG | 1 cut(s) | 1165 |
| HhaI | GCGC | 2 cut(s) | 471, 849 |
| Hin1II | CATG | 9 cut(s) | 148, 346, 392, 429, 486, 874, 1020, 1570, 1635 |
| Hin6I | GCGC | 2 cut(s) | 469, 847 |
| HinP1I | GCGC | 2 cut(s) | 469, 847 |
| HincII | GTYRAC | 1 cut(s) | 499 |
| HindII | GTYRAC | 1 cut(s) | 499 |
| HindIII | AAGCTT | 2 cut(s) | 296, 1477 |
| HinfI | GANTC | 6 cut(s) | 259, 685, 765, 1141, 1169, 1246 |
| HpaII | CCGG | 1 cut(s) | 1165 |
| HphI | GGTGA | 3 cut(s) | 382, 941, 1273 |
| Hpy166II | GTNNAC | 4 cut(s) | 499, 705, 1153, 1216 |
| Hpy188I | TCNGA | 4 cut(s) | 666, 976, 1446, 1607 |
| Hpy188III | TCNNGA | 8 cut(s) | 434, 672, 871, 879, 1228, 1520, 1567, 1590 |
| Hpy8I | GTNNAC | 4 cut(s) | 499, 705, 1153, 1216 |
| Hpy99I | CGWCG | 1 cut(s) | 66 |
| HpyAV | CCTTC | 4 cut(s) | 576, 971, 1062, 1551 |
| HpyCH4III | ACNGT | 6 cut(s) | 169, 238, 314, 544, 1114, 1204 |
| HpyCH4IV | ACGT | 3 cut(s) | 1272, 1536, 1702 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 146, 249, 827, 846, 1499 |
| HpyF3I | CTNAG | 4 cut(s) | 29, 826, 954, 1103 |
| HpySE526I | ACGT | 3 cut(s) | 1272, 1536, 1702 |
| Hsp92II | CATG | 9 cut(s) | 148, 346, 392, 429, 486, 874, 1020, 1570, 1635 |
| HspAI | GCGC | 2 cut(s) | 469, 847 |
| Kzo9I | GATC | 4 cut(s) | 661, 718, 873, 1438 |
| LmnI | GCTCC | 2 cut(s) | 63, 752 |
| Lsp1109I | GCAGC | 8 cut(s) | 20, 56, 213, 363, 805, 806, 1502, 1648 |
| LweI | GCATC | 7 cut(s) | 456, 562, 858, 1000, 1183, 1268, 1489 |
| MaeI | CTAG | 3 cut(s) | 119, 1514, 1547 |
| MaeII | ACGT | 3 cut(s) | 1272, 1536, 1702 |
| MaeIII | GTNAC | 2 cut(s) | 682, 983 |
| MalI | GATC | 4 cut(s) | 663, 720, 875, 1440 |
| MboI | GATC | 4 cut(s) | 661, 718, 873, 1438 |
| MfeI | CAATTG | 1 cut(s) | 630 |
| MflI | RGATCY | 2 cut(s) | 661, 1438 |
| MluCI | AATT | 9 cut(s) | 356, 411, 630, 654, 834, 861, 1031, 1035, 1304 |
| MlyI | GAGTC | 2 cut(s) | 679, 1163 |
| Mph1103I | ATGCAT | 1 cut(s) | 577 |
| MroXI | GAANNNNTTC | 2 cut(s) | 215, 1440 |
| MseI | TTAA | 9 cut(s) | 590, 597, 618, 1034, 1053, 1176, 1485, 1509, 1584 |
| MslI | CAYNNNNRTG | 1 cut(s) | 710 |
| MspA1I | CMGCKG | 1 cut(s) | 1493 |
| MspCI | CTTAAG | 1 cut(s) | 1175 |
| MspI | CCGG | 1 cut(s) | 1165 |
| MspR9I | CCNGG | 2 cut(s) | 100, 1166 |
| MunI | CAATTG | 1 cut(s) | 630 |
| Mva1269I | GAATGC | 3 cut(s) | 534, 1673, 1693 |
| MvaI | CCWGG | 1 cut(s) | 100 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 146, 249, 827, 846, 1499 |
| NciI | CCSGG | 1 cut(s) | 1166 |
| NdeI | CATATG | 1 cut(s) | 473 |
| NdeII | GATC | 4 cut(s) | 661, 718, 873, 1438 |
| NlaIII | CATG | 9 cut(s) | 148, 346, 392, 429, 486, 874, 1020, 1570, 1635 |
| NlaIV | GGNNCC | 2 cut(s) | 287, 1022 |
| NmuCI | GTSAC | 2 cut(s) | 682, 983 |
| NsbI | TGCGCA | 1 cut(s) | 470 |
| NsiI | ATGCAT | 1 cut(s) | 577 |
| NspI | RCATGY | 2 cut(s) | 346, 392 |
| NspV | TTCGAA | 1 cut(s) | 1328 |
| PaeR7I | CTCGAG | 1 cut(s) | 1673 |
| PagI | TCATGA | 2 cut(s) | 870, 1566 |
| PciI | ACATGT | 1 cut(s) | 342 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 733, 742, 1431 |
| PctI | GAATGC | 3 cut(s) | 534, 1673, 1693 |
| PdmI | GAANNNNTTC | 2 cut(s) | 215, 1440 |
| PfeI | GAWTC | 4 cut(s) | 259, 765, 1141, 1246 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 901 |
| PfoI | TCCNGGA | 1 cut(s) | 1164 |
| PkrI | GCNGC | 8 cut(s) | 35, 71, 228, 353, 796, 820, 1492, 1638 |
| PleI | GAGTC | 2 cut(s) | 679, 1163 |
| PpsI | GAGTC | 2 cut(s) | 679, 1163 |
| PscI | ACATGT | 1 cut(s) | 342 |
| PshBI | ATTAAT | 2 cut(s) | 618, 1034 |
| PsiI | TTATAA | 1 cut(s) | 1208 |
| Psp1406I | AACGTT | 1 cut(s) | 1702 |
| Psp6I | CCWGG | 1 cut(s) | 98 |
| PspGI | CCWGG | 1 cut(s) | 98 |
| PspN4I | GGNNCC | 2 cut(s) | 287, 1022 |
| PspPI | GGNCC | 2 cut(s) | 286, 1595 |
| PspXI | VCTCGAGB | 1 cut(s) | 1673 |
| PstI | CTGCAG | 2 cut(s) | 970, 1663 |
| PsuI | RGATCY | 2 cut(s) | 661, 1438 |
| PvuII | CAGCTG | 1 cut(s) | 1493 |
| RsaI | GTAC | 6 cut(s) | 171, 856, 1215, 1275, 1432, 1506 |
| RsaNI | GTAC | 6 cut(s) | 170, 855, 1214, 1274, 1431, 1505 |
| RseI | CAYNNNNRTG | 1 cut(s) | 710 |
| SaqAI | TTAA | 9 cut(s) | 590, 597, 618, 1034, 1053, 1176, 1485, 1509, 1584 |
| SatI | GCNGC | 8 cut(s) | 34, 70, 227, 352, 795, 819, 1491, 1637 |
| Sau3AI | GATC | 4 cut(s) | 661, 718, 873, 1438 |
| Sau96I | GGNCC | 2 cut(s) | 286, 1595 |
| SchI | GAGTC | 2 cut(s) | 679, 1163 |
| ScrFI | CCNGG | 2 cut(s) | 100, 1166 |
| SfaNI | GCATC | 7 cut(s) | 456, 562, 858, 1000, 1183, 1268, 1489 |
| SfcI | CTRYAG | 3 cut(s) | 254, 966, 1659 |
| Sfr274I | CTCGAG | 1 cut(s) | 1673 |
| SfuI | TTCGAA | 1 cut(s) | 1328 |
| SinI | GGWCC | 2 cut(s) | 286, 1595 |
| SlaI | CTCGAG | 1 cut(s) | 1673 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 710 |
| SmlI | CTYRAG | 3 cut(s) | 502, 1175, 1673 |
| SmoI | CTYRAG | 3 cut(s) | 502, 1175, 1673 |
| Sse9I | AATT | 9 cut(s) | 356, 411, 630, 654, 834, 861, 1031, 1035, 1304 |
| SsiI | CCGC | 4 cut(s) | 178, 279, 1471, 1544 |
| SspMI | CTAG | 3 cut(s) | 119, 1514, 1547 |
| StyD4I | CCNGG | 2 cut(s) | 98, 1164 |
| TaaI | ACNGT | 6 cut(s) | 169, 238, 314, 544, 1114, 1204 |
| TaiI | ACGT | 3 cut(s) | 1275, 1539, 1705 |
| TaqI | TCGA | 9 cut(s) | 64, 219, 736, 1227, 1328, 1425, 1464, 1655, 1674 |
| TasI | AATT | 9 cut(s) | 356, 411, 630, 654, 834, 861, 1031, 1035, 1304 |
| TatI | WGTACW | 1 cut(s) | 1213 |
| TfiI | GAWTC | 4 cut(s) | 259, 765, 1141, 1246 |
| Tru1I | TTAA | 9 cut(s) | 590, 597, 618, 1034, 1053, 1176, 1485, 1509, 1584 |
| Tru9I | TTAA | 9 cut(s) | 590, 597, 618, 1034, 1053, 1176, 1485, 1509, 1584 |
| TscAI | CASTG | 2 cut(s) | 1195, 1248 |
| TseFI | GTSAC | 2 cut(s) | 682, 983 |
| TseI | GCWGC | 8 cut(s) | 33, 69, 226, 351, 794, 818, 1490, 1636 |
| Tsp45I | GTSAC | 2 cut(s) | 682, 983 |
| TspDTI | ATGAA | 7 cut(s) | 213, 757, 792, 822, 1074, 1583, 1680 |
| TspGWI | ACGGA | 3 cut(s) | 50, 278, 734 |
| TspRI | CASTG | 2 cut(s) | 1195, 1248 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 901 |
| Vha464I | CTTAAG | 1 cut(s) | 1175 |
| VpaK11BI | GGWCC | 2 cut(s) | 286, 1595 |
| VspI | ATTAAT | 2 cut(s) | 618, 1034 |
| XapI | RAATTY | 2 cut(s) | 356, 1304 |
| XceI | RCATGY | 2 cut(s) | 346, 392 |
| XhoI | CTCGAG | 1 cut(s) | 1673 |
| XmnI | GAANNNNTTC | 2 cut(s) | 215, 1440 |
| XspI | CTAG | 3 cut(s) | 119, 1514, 1547 |
| Zsp2I | ATGCAT | 1 cut(s) | 577 |