Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 96, 1043 |
| AarI | CACCTGC | 1 cut(s) | 113 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 538 |
| Acc16I | TGCGCA | 1 cut(s) | 317 |
| Acc36I | ACCTGC | 1 cut(s) | 113 |
| Acc65I | GGTACC | 1 cut(s) | 404 |
| AccB1I | GGYRCC | 1 cut(s) | 404 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 575 |
| AciI | CCGC | 2 cut(s) | 812, 1085 |
| AclWI | GGATC | 8 cut(s) | 441, 560, 573, 914, 1089, 1102, 1262, 1305 |
| AcsI | RAATTY | 2 cut(s) | 381, 988 |
| AcuI | CTGAAG | 5 cut(s) | 726, 751, 1146, 1467, 1512 |
| AfaI | GTAC | 6 cut(s) | 227, 365, 406, 953, 1197, 1535 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 415, 575, 865, 1091 |
| AgsI | TTSAA | 8 cut(s) | 38, 67, 103, 482, 626, 675, 1176, 1531 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1433 |
| AhlI | ACTAGT | 1 cut(s) | 619 |
| Alw21I | GWGCWC | 1 cut(s) | 1114 |
| Alw44I | GTGCAC | 1 cut(s) | 1110 |
| AlwI | GGATC | 8 cut(s) | 441, 560, 573, 914, 1089, 1102, 1262, 1305 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1037, 1490 |
| AoxI | GGCC | 1 cut(s) | 875 |
| ApaI | GGGCCC | 1 cut(s) | 879 |
| ApaLI | GTGCAC | 1 cut(s) | 1110 |
| ApeKI | GCWGC | 7 cut(s) | 313, 641, 644, 665, 920, 1249, 1468 |
| ApoI | RAATTY | 2 cut(s) | 381, 988 |
| AseI | ATTAAT | 1 cut(s) | 1401 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1523 |
| Asp718I | GGTACC | 1 cut(s) | 404 |
| AspA2I | CCTAGG | 1 cut(s) | 1345 |
| AspLEI | GCGC | 3 cut(s) | 10, 318, 1331 |
| AspS9I | GGNCC | 6 cut(s) | 133, 489, 875, 876, 1411, 1427 |
| AsuC2I | CCSGG | 2 cut(s) | 410, 520 |
| AsuHPI | GGTGA | 3 cut(s) | 534, 826, 997 |
| AvaII | GGWCC | 4 cut(s) | 133, 489, 1411, 1427 |
| AvrII | CCTAGG | 1 cut(s) | 1345 |
| BaeGI | GKGCMC | 2 cut(s) | 879, 1114 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 318, 351 |
| BamHI | GGATCC | 2 cut(s) | 565, 1094 |
| BanI | GGYRCC | 1 cut(s) | 404 |
| BanII | GRGCYC | 1 cut(s) | 879 |
| BbsI | GAAGAC | 3 cut(s) | 110, 489, 785 |
| Bbv12I | GWGCWC | 1 cut(s) | 1114 |
| BbvI | GCAGC | 7 cut(s) | 300, 628, 631, 677, 932, 1236, 1480 |
| BccI | CCATC | 4 cut(s) | 123, 187, 823, 1195 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 233, 267 |
| BcnI | CCSGG | 2 cut(s) | 410, 520 |
| BcuI | ACTAGT | 1 cut(s) | 619 |
| BfaI | CTAG | 3 cut(s) | 620, 1346, 1537 |
| BfoI | RGCGCY | 1 cut(s) | 1332 |
| BfuAI | ACCTGC | 1 cut(s) | 113 |
| BglII | AGATCT | 2 cut(s) | 60, 508 |
| BisI | GCNGC | 7 cut(s) | 314, 642, 645, 666, 921, 1250, 1469 |
| BlnI | CCTAGG | 1 cut(s) | 1345 |
| BlsI | GCNGC | 7 cut(s) | 315, 643, 646, 667, 922, 1251, 1470 |
| BmcAI | AGTACT | 2 cut(s) | 1197, 1535 |
| Bme1390I | CCNGG | 2 cut(s) | 410, 520 |
| Bme18I | GGWCC | 4 cut(s) | 133, 489, 1411, 1427 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1433 |
| BmgT120I | GGNCC | 6 cut(s) | 133, 489, 875, 876, 1411, 1427 |
| BmiI | GGNNCC | 6 cut(s) | 134, 406, 567, 877, 1096, 1141 |
| BmrFI | CCNGG | 2 cut(s) | 410, 520 |
| BmsI | GCATC | 3 cut(s) | 409, 510, 1018 |
| BpiI | GAAGAC | 3 cut(s) | 110, 489, 785 |
| BpmI | CTGGAG | 1 cut(s) | 1443 |
| BpuEI | CTTGAG | 1 cut(s) | 995 |
| BpuMI | CCSGG | 2 cut(s) | 410, 520 |
| BsaJI | CCNNGG | 2 cut(s) | 408, 1345 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 693, 723, 1416, 1446 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 415, 575, 865, 1091 |
| Bse1I | ACTGG | 5 cut(s) | 491, 569, 648, 1098, 1430 |
| Bse3DI | GCAATG | 4 cut(s) | 169, 174, 429, 1244 |
| BseDI | CCNNGG | 2 cut(s) | 408, 1345 |
| BseGI | GGATG | 6 cut(s) | 134, 424, 787, 1372, 1477, 1518 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 415, 575, 865, 1091 |
| BseMI | GCAATG | 4 cut(s) | 169, 174, 429, 1244 |
| BseMII | CTCAG | 3 cut(s) | 815, 1204, 1396 |
| BseNI | ACTGG | 5 cut(s) | 491, 569, 648, 1098, 1430 |
| BseRI | GAGGAG | 1 cut(s) | 1109 |
| BseSI | GKGCMC | 2 cut(s) | 879, 1114 |
| BseXI | GCAGC | 7 cut(s) | 300, 628, 631, 677, 932, 1236, 1480 |
| BseYI | CCCAGC | 1 cut(s) | 76 |
| Bsh1285I | CGRYCG | 1 cut(s) | 948 |
| BshFI | GGCC | 1 cut(s) | 877 |
| BshNI | GGYRCC | 1 cut(s) | 404 |
| BsiEI | CGRYCG | 1 cut(s) | 948 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1114 |
| BsiSI | CCGG | 2 cut(s) | 410, 519 |
| BslFI | GGGAC | 2 cut(s) | 41, 1501 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 415, 575, 865, 1091 |
| BsmFI | GGGAC | 2 cut(s) | 41, 1501 |
| BsmI | GAATGC | 2 cut(s) | 164, 1505 |
| BsnI | GGCC | 1 cut(s) | 877 |
| Bsp120I | GGGCCC | 1 cut(s) | 875 |
| Bsp1286I | GDGCHC | 2 cut(s) | 879, 1114 |
| Bsp1407I | TGTACA | 1 cut(s) | 951 |
| BspACI | CCGC | 2 cut(s) | 812, 1085 |
| BspANI | GGCC | 1 cut(s) | 877 |
| BspCNI | CTCAG | 3 cut(s) | 814, 1203, 1395 |
| BspHI | TCATGA | 1 cut(s) | 717 |
| BspLI | GGNNCC | 6 cut(s) | 134, 406, 567, 877, 1096, 1141 |
| BspMI | ACCTGC | 1 cut(s) | 113 |
| BspPI | GGATC | 8 cut(s) | 441, 560, 573, 914, 1089, 1102, 1262, 1305 |
| BspT107I | GGYRCC | 1 cut(s) | 404 |
| BsrDI | GCAATG | 4 cut(s) | 169, 174, 429, 1244 |
| BsrGI | TGTACA | 1 cut(s) | 951 |
| BsrI | ACTGG | 5 cut(s) | 491, 569, 648, 1098, 1430 |
| BssECI | CCNNGG | 2 cut(s) | 408, 1345 |
| BssT1I | CCWWGG | 1 cut(s) | 1345 |
| Bst4CI | ACNGT | 7 cut(s) | 368, 449, 759, 949, 1039, 1240, 1321 |
| Bst6I | CTCTTC | 2 cut(s) | 354, 1488 |
| BstAUI | TGTACA | 1 cut(s) | 951 |
| BstC8I | GCNNGC | 2 cut(s) | 585, 1473 |
| BstDEI | CTNAG | 4 cut(s) | 801, 938, 1190, 1382 |
| BstF5I | GGATG | 6 cut(s) | 134, 424, 787, 1372, 1477, 1518 |
| BstH2I | RGCGCY | 1 cut(s) | 1332 |
| BstHHI | GCGC | 3 cut(s) | 10, 318, 1331 |
| BstMCI | CGRYCG | 1 cut(s) | 948 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 82, 580 |
| BstSCI | CCNGG | 2 cut(s) | 408, 518 |
| BstSLI | GKGCMC | 2 cut(s) | 879, 1114 |
| BstV1I | GCAGC | 7 cut(s) | 300, 628, 631, 677, 932, 1236, 1480 |
| BstV2I | GAAGAC | 3 cut(s) | 110, 489, 785 |
| BstX2I | RGATCY | 7 cut(s) | 60, 433, 508, 565, 906, 1094, 1267 |
| BstYI | RGATCY | 7 cut(s) | 60, 433, 508, 565, 906, 1094, 1267 |
| BsuRI | GGCC | 1 cut(s) | 877 |
| BtgZI | GCGATG | 1 cut(s) | 1041 |
| BtsCI | GGATG | 6 cut(s) | 134, 424, 787, 1372, 1477, 1518 |
| BtsIMutI | CAGTG | 6 cut(s) | 576, 655, 809, 1105, 1390, 1444 |
| BveI | ACCTGC | 1 cut(s) | 113 |
| Cac8I | GCNNGC | 2 cut(s) | 585, 1473 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1037, 1490 |
| CciI | TCATGA | 1 cut(s) | 717 |
| CfoI | GCGC | 3 cut(s) | 10, 318, 1331 |
| Cfr13I | GGNCC | 6 cut(s) | 133, 489, 875, 876, 1411, 1427 |
| Csp6I | GTAC | 6 cut(s) | 226, 364, 405, 952, 1196, 1534 |
| CviAII | CATG | 5 cut(s) | 31, 229, 330, 718, 1464 |
| CviQI | GTAC | 6 cut(s) | 226, 364, 405, 952, 1196, 1534 |
| DdeI | CTNAG | 4 cut(s) | 801, 938, 1190, 1382 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 538 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1433 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 538 |
| Eam1104I | CTCTTC | 2 cut(s) | 354, 1488 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1433 |
| EarI | CTCTTC | 2 cut(s) | 354, 1488 |
| Eco130I | CCWWGG | 1 cut(s) | 1345 |
| Eco24I | GRGCYC | 1 cut(s) | 879 |
| Eco32I | GATATC | 1 cut(s) | 723 |
| Eco47I | GGWCC | 4 cut(s) | 133, 489, 1411, 1427 |
| Eco57I | CTGAAG | 5 cut(s) | 726, 751, 1146, 1467, 1512 |
| EcoO109I | RGGNCCY | 1 cut(s) | 876 |
| EcoRI | GAATTC | 1 cut(s) | 381 |
| EcoRV | GATATC | 1 cut(s) | 723 |
| EcoT14I | CCWWGG | 1 cut(s) | 1345 |
| EcoT22I | ATGCAT | 1 cut(s) | 1060 |
| EcoT38I | GRGCYC | 1 cut(s) | 879 |
| ErhI | CCWWGG | 1 cut(s) | 1345 |
| FaeI | CATG | 5 cut(s) | 34, 232, 333, 721, 1467 |
| FaqI | GGGAC | 2 cut(s) | 41, 1501 |
| FatI | CATG | 5 cut(s) | 30, 228, 329, 717, 1463 |
| Fnu4HI | GCNGC | 7 cut(s) | 314, 642, 645, 666, 921, 1250, 1469 |
| FokI | GGATG | 6 cut(s) | 141, 431, 794, 1379, 1464, 1525 |
| FriOI | GRGCYC | 1 cut(s) | 879 |
| Fsp4HI | GCNGC | 7 cut(s) | 314, 642, 645, 666, 921, 1250, 1469 |
| FspBI | CTAG | 3 cut(s) | 620, 1346, 1537 |
| FspI | TGCGCA | 1 cut(s) | 317 |
| GlaI | GCGC | 3 cut(s) | 9, 317, 1330 |
| GluI | GCNGC | 7 cut(s) | 314, 642, 645, 666, 921, 1250, 1469 |
| GsaI | CCCAGC | 1 cut(s) | 80 |
| GsuI | CTGGAG | 1 cut(s) | 1443 |
| HaeII | RGCGCY | 1 cut(s) | 1332 |
| HaeIII | GGCC | 1 cut(s) | 877 |
| HapII | CCGG | 2 cut(s) | 410, 519 |
| HhaI | GCGC | 3 cut(s) | 10, 318, 1331 |
| Hin1II | CATG | 5 cut(s) | 34, 232, 333, 721, 1467 |
| Hin6I | GCGC | 3 cut(s) | 8, 316, 1329 |
| HinP1I | GCGC | 3 cut(s) | 8, 316, 1329 |
| HindIII | AAGCTT | 1 cut(s) | 143 |
| HinfI | GANTC | 4 cut(s) | 532, 612, 845, 1181 |
| HpaII | CCGG | 2 cut(s) | 410, 519 |
| HphI | GGTGA | 3 cut(s) | 534, 826, 997 |
| Hpy166II | GTNNAC | 5 cut(s) | 346, 809, 952, 1112, 1276 |
| Hpy188I | TCNGA | 3 cut(s) | 820, 1193, 1264 |
| Hpy188III | TCNNGA | 4 cut(s) | 718, 1012, 1409, 1422 |
| Hpy8I | GTNNAC | 5 cut(s) | 346, 809, 952, 1112, 1276 |
| Hpy99I | CGWCG | 1 cut(s) | 47 |
| HpyAV | CCTTC | 4 cut(s) | 423, 655, 897, 1491 |
| HpyCH4III | ACNGT | 7 cut(s) | 368, 449, 759, 949, 1039, 1240, 1321 |
| HpyCH4IV | ACGT | 1 cut(s) | 352 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 82, 580 |
| HpyF3I | CTNAG | 4 cut(s) | 801, 938, 1190, 1382 |
| HpySE526I | ACGT | 1 cut(s) | 352 |
| Hsp92II | CATG | 5 cut(s) | 34, 232, 333, 721, 1467 |
| HspAI | GCGC | 3 cut(s) | 8, 316, 1329 |
| KpnI | GGTACC | 1 cut(s) | 408 |
| LmnI | GCTCC | 3 cut(s) | 152, 701, 1103 |
| Lsp1109I | GCAGC | 7 cut(s) | 300, 628, 631, 677, 932, 1236, 1480 |
| LweI | GCATC | 3 cut(s) | 409, 510, 1018 |
| MaeI | CTAG | 3 cut(s) | 620, 1346, 1537 |
| MaeII | ACGT | 1 cut(s) | 352 |
| MaeIII | GTNAC | 4 cut(s) | 540, 832, 854, 1168 |
| MflI | RGATCY | 7 cut(s) | 60, 433, 508, 565, 906, 1094, 1267 |
| MhlI | GDGCHC | 2 cut(s) | 879, 1114 |
| MluCI | AATT | 8 cut(s) | 98, 381, 696, 870, 988, 1221, 1335, 1402 |
| MlyI | GAGTC | 2 cut(s) | 526, 854 |
| Mph1103I | ATGCAT | 1 cut(s) | 1060 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1523 |
| MseI | TTAA | 4 cut(s) | 1224, 1353, 1401, 1416 |
| MspI | CCGG | 2 cut(s) | 410, 519 |
| MspR9I | CCNGG | 2 cut(s) | 410, 520 |
| Mva1269I | GAATGC | 2 cut(s) | 164, 1505 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 82, 580 |
| NciI | CCSGG | 2 cut(s) | 410, 520 |
| NlaIII | CATG | 5 cut(s) | 34, 232, 333, 721, 1467 |
| NlaIV | GGNNCC | 6 cut(s) | 134, 406, 567, 877, 1096, 1141 |
| NmuCI | GTSAC | 2 cut(s) | 540, 832 |
| NsbI | TGCGCA | 1 cut(s) | 317 |
| NsiI | ATGCAT | 1 cut(s) | 1060 |
| PagI | TCATGA | 1 cut(s) | 717 |
| PaqCI | CACCTGC | 1 cut(s) | 113 |
| PctI | GAATGC | 2 cut(s) | 164, 1505 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1523 |
| PfeI | GAWTC | 2 cut(s) | 612, 1181 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 575 |
| PfoI | TCCNGGA | 1 cut(s) | 518 |
| PkrI | GCNGC | 7 cut(s) | 315, 643, 646, 667, 922, 1251, 1470 |
| PleI | GAGTC | 2 cut(s) | 526, 853 |
| PpsI | GAGTC | 2 cut(s) | 526, 853 |
| PshBI | ATTAAT | 1 cut(s) | 1401 |
| PsiI | TTATAA | 2 cut(s) | 96, 1043 |
| PspFI | CCCAGC | 1 cut(s) | 76 |
| PspN4I | GGNNCC | 6 cut(s) | 134, 406, 567, 877, 1096, 1141 |
| PspOMI | GGGCCC | 1 cut(s) | 875 |
| PspPI | GGNCC | 6 cut(s) | 133, 489, 875, 876, 1411, 1427 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1037, 1490 |
| PsuI | RGATCY | 7 cut(s) | 60, 433, 508, 565, 906, 1094, 1267 |
| RsaI | GTAC | 6 cut(s) | 227, 365, 406, 953, 1197, 1535 |
| RsaNI | GTAC | 6 cut(s) | 226, 364, 405, 952, 1196, 1534 |
| SaqAI | TTAA | 4 cut(s) | 1224, 1353, 1401, 1416 |
| SatI | GCNGC | 7 cut(s) | 314, 642, 645, 666, 921, 1250, 1469 |
| Sau96I | GGNCC | 6 cut(s) | 133, 489, 875, 876, 1411, 1427 |
| ScaI | AGTACT | 2 cut(s) | 1197, 1535 |
| SchI | GAGTC | 2 cut(s) | 526, 854 |
| ScrFI | CCNGG | 2 cut(s) | 410, 520 |
| SduI | GDGCHC | 2 cut(s) | 879, 1114 |
| SfaNI | GCATC | 3 cut(s) | 409, 510, 1018 |
| SinI | GGWCC | 4 cut(s) | 133, 489, 1411, 1427 |
| SmlI | CTYRAG | 1 cut(s) | 1010 |
| SmoI | CTYRAG | 1 cut(s) | 1010 |
| SpeI | ACTAGT | 1 cut(s) | 619 |
| Sse9I | AATT | 8 cut(s) | 98, 381, 696, 870, 988, 1221, 1335, 1402 |
| SsiI | CCGC | 2 cut(s) | 812, 1085 |
| SspI | AATATT | 1 cut(s) | 72 |
| SspMI | CTAG | 3 cut(s) | 620, 1346, 1537 |
| StyD4I | CCNGG | 2 cut(s) | 408, 518 |
| StyI | CCWWGG | 1 cut(s) | 1345 |
| TaaI | ACNGT | 7 cut(s) | 368, 449, 759, 949, 1039, 1240, 1321 |
| TaiI | ACGT | 1 cut(s) | 355 |
| TaqI | TCGA | 3 cut(s) | 42, 253, 1300 |
| TasI | AATT | 8 cut(s) | 98, 381, 696, 870, 988, 1221, 1335, 1402 |
| TatI | WGTACW | 5 cut(s) | 225, 363, 951, 1195, 1533 |
| TfiI | GAWTC | 2 cut(s) | 612, 1181 |
| Tru1I | TTAA | 4 cut(s) | 1224, 1353, 1401, 1416 |
| Tru9I | TTAA | 4 cut(s) | 1224, 1353, 1401, 1416 |
| TscAI | CASTG | 6 cut(s) | 576, 655, 809, 1105, 1390, 1444 |
| TseFI | GTSAC | 2 cut(s) | 540, 832 |
| TseI | GCWGC | 7 cut(s) | 313, 641, 644, 665, 920, 1249, 1468 |
| Tsp45I | GTSAC | 2 cut(s) | 540, 832 |
| TspDTI | ATGAA | 6 cut(s) | 245, 669, 785, 909, 982, 1529 |
| TspGWI | ACGGA | 3 cut(s) | 125, 779, 1406 |
| TspRI | CASTG | 6 cut(s) | 576, 655, 809, 1105, 1390, 1444 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 575 |
| VneI | GTGCAC | 1 cut(s) | 1110 |
| VpaK11BI | GGWCC | 4 cut(s) | 133, 489, 1411, 1427 |
| VspI | ATTAAT | 1 cut(s) | 1401 |
| XapI | RAATTY | 2 cut(s) | 381, 988 |
| XmaJI | CCTAGG | 1 cut(s) | 1345 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1523 |
| XspI | CTAG | 3 cut(s) | 620, 1346, 1537 |
| ZrmI | AGTACT | 2 cut(s) | 1197, 1535 |
| Zsp2I | ATGCAT | 1 cut(s) | 1060 |