Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 298, 478 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 126 |
| AccI | GTMKAC | 2 cut(s) | 229, 983 |
| AccII | CGCG | 1 cut(s) | 312 |
| AccIII | TCCGGA | 1 cut(s) | 458 |
| AciI | CCGC | 2 cut(s) | 179, 310 |
| AcuI | CTGAAG | 2 cut(s) | 505, 1268 |
| AdeI | CACNNNGTG | 1 cut(s) | 539 |
| AfaI | GTAC | 5 cut(s) | 146, 578, 589, 834, 1270 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 121, 126, 551 |
| AgsI | TTSAA | 5 cut(s) | 112, 502, 605, 628, 1266 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1166 |
| AjnI | CCWGG | 1 cut(s) | 265 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 699, 731 |
| Alw21I | GWGCWC | 2 cut(s) | 13, 45 |
| Alw44I | GTGCAC | 1 cut(s) | 41 |
| Ama87I | CYCGRG | 1 cut(s) | 241 |
| Aor13HI | TCCGGA | 1 cut(s) | 458 |
| AoxI | GGCC | 3 cut(s) | 180, 211, 1065 |
| ApaLI | GTGCAC | 1 cut(s) | 41 |
| ApeKI | GCWGC | 7 cut(s) | 164, 374, 377, 398, 659, 1144, 1201 |
| AseI | ATTAAT | 1 cut(s) | 783 |
| AspS9I | GGNCC | 4 cut(s) | 181, 1065, 1160, 1304 |
| AsuC2I | CCSGG | 1 cut(s) | 60 |
| AsuHPI | GGTGA | 1 cut(s) | 289 |
| AvaI | CYCGRG | 1 cut(s) | 241 |
| AvaII | GGWCC | 2 cut(s) | 1160, 1304 |
| BaeGI | GKGCMC | 2 cut(s) | 45, 618 |
| BanII | GRGCYC | 1 cut(s) | 13 |
| BbsI | GAAGAC | 3 cut(s) | 511, 518, 689 |
| Bbv12I | GWGCWC | 2 cut(s) | 13, 45 |
| BbvI | GCAGC | 7 cut(s) | 176, 361, 364, 385, 646, 1156, 1213 |
| BccI | CCATC | 1 cut(s) | 553 |
| BceAI | ACGGC | 2 cut(s) | 150, 1093 |
| BciT130I | CCWGG | 1 cut(s) | 267 |
| BcnI | CCSGG | 1 cut(s) | 60 |
| BfaI | CTAG | 4 cut(s) | 14, 1079, 1085, 1272 |
| BfmI | CTRYAG | 2 cut(s) | 222, 1224 |
| BfuAI | ACCTGC | 2 cut(s) | 298, 478 |
| BglII | AGATCT | 1 cut(s) | 1274 |
| BisI | GCNGC | 8 cut(s) | 165, 180, 375, 378, 399, 660, 1145, 1202 |
| BlpI | GCTNAGC | 1 cut(s) | 86 |
| BlsI | GCNGC | 8 cut(s) | 166, 181, 376, 379, 400, 661, 1146, 1203 |
| BmcAI | AGTACT | 1 cut(s) | 1270 |
| Bme1390I | CCNGG | 2 cut(s) | 60, 267 |
| Bme18I | GGWCC | 2 cut(s) | 1160, 1304 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1166 |
| BmeT110I | CYCGRG | 1 cut(s) | 241 |
| BmgT120I | GGNCC | 4 cut(s) | 181, 1065, 1160, 1304 |
| BmiI | GGNNCC | 1 cut(s) | 1306 |
| BmrFI | CCNGG | 2 cut(s) | 60, 267 |
| BmsI | GCATC | 4 cut(s) | 545, 757, 1126, 1223 |
| BpiI | GAAGAC | 3 cut(s) | 511, 518, 689 |
| BpmI | CTGGAG | 2 cut(s) | 1176, 1302 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 86 |
| BpuEI | CTTGAG | 1 cut(s) | 1223 |
| BpuMI | CCSGG | 1 cut(s) | 60 |
| BsaJI | CCNNGG | 1 cut(s) | 120 |
| BsaWI | WCCGGW | 1 cut(s) | 458 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 121, 126, 551 |
| Bse3DI | GCAATG | 2 cut(s) | 486, 669 |
| BseAI | TCCGGA | 1 cut(s) | 458 |
| BseBI | CCWGG | 1 cut(s) | 267 |
| BseDI | CCNNGG | 1 cut(s) | 120 |
| BseGI | GGATG | 7 cut(s) | 529, 560, 850, 1105, 1141, 1210, 1238 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 121, 126, 551 |
| BseMI | GCAATG | 2 cut(s) | 486, 669 |
| BseMII | CTCAG | 1 cut(s) | 92 |
| BseSI | GKGCMC | 2 cut(s) | 45, 618 |
| BseXI | GCAGC | 7 cut(s) | 176, 361, 364, 385, 646, 1156, 1213 |
| Bsh1236I | CGCG | 1 cut(s) | 312 |
| BshFI | GGCC | 3 cut(s) | 182, 213, 1067 |
| BsiHKAI | GWGCWC | 2 cut(s) | 13, 45 |
| BsiHKCI | CYCGRG | 1 cut(s) | 241 |
| BsiSI | CCGG | 2 cut(s) | 60, 459 |
| BslFI | GGGAC | 4 cut(s) | 259, 755, 875, 1234 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 121, 126, 551 |
| BsmFI | GGGAC | 4 cut(s) | 259, 755, 875, 1234 |
| BsmI | GAATGC | 1 cut(s) | 236 |
| BsnI | GGCC | 3 cut(s) | 182, 213, 1067 |
| BsoBI | CYCGRG | 1 cut(s) | 241 |
| Bsp1286I | GDGCHC | 3 cut(s) | 13, 45, 618 |
| Bsp13I | TCCGGA | 1 cut(s) | 458 |
| Bsp143I | GATC | 5 cut(s) | 445, 453, 1033, 1128, 1274 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 86 |
| Bsp19I | CCATGG | 1 cut(s) | 120 |
| BspACI | CCGC | 2 cut(s) | 179, 310 |
| BspANI | GGCC | 3 cut(s) | 182, 213, 1067 |
| BspCNI | CTCAG | 1 cut(s) | 91 |
| BspEI | TCCGGA | 1 cut(s) | 458 |
| BspFNI | CGCG | 1 cut(s) | 312 |
| BspLI | GGNNCC | 1 cut(s) | 1306 |
| BspMAI | CTGCAG | 1 cut(s) | 1228 |
| BspMI | ACCTGC | 2 cut(s) | 298, 478 |
| BsrDI | GCAATG | 2 cut(s) | 486, 669 |
| BssECI | CCNNGG | 1 cut(s) | 120 |
| BssMI | GATC | 5 cut(s) | 445, 453, 1033, 1128, 1274 |
| BssNAI | GTATAC | 1 cut(s) | 230 |
| BssT1I | CCWWGG | 1 cut(s) | 120 |
| Bst1107I | GTATAC | 1 cut(s) | 230 |
| Bst2UI | CCWGG | 1 cut(s) | 267 |
| Bst4CI | ACNGT | 3 cut(s) | 537, 563, 778 |
| Bst6I | CTCTTC | 3 cut(s) | 77, 868, 1267 |
| BstC8I | GCNNGC | 3 cut(s) | 19, 23, 1206 |
| BstDEI | CTNAG | 4 cut(s) | 78, 86, 677, 1009 |
| BstDSI | CCRYGG | 1 cut(s) | 120 |
| BstEII | GGTNACC | 1 cut(s) | 95 |
| BstF5I | GGATG | 7 cut(s) | 529, 560, 850, 1105, 1141, 1210, 1238 |
| BstFNI | CGCG | 1 cut(s) | 312 |
| BstKTI | GATC | 5 cut(s) | 448, 456, 1036, 1131, 1277 |
| BstMBI | GATC | 5 cut(s) | 445, 453, 1033, 1128, 1274 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 407, 1064, 1186 |
| BstNI | CCWGG | 1 cut(s) | 267 |
| BstNSI | RCATGY | 1 cut(s) | 21 |
| BstPI | GGTNACC | 1 cut(s) | 95 |
| BstSCI | CCNGG | 2 cut(s) | 58, 265 |
| BstSFI | CTRYAG | 2 cut(s) | 222, 1224 |
| BstSLI | GKGCMC | 2 cut(s) | 45, 618 |
| BstUI | CGCG | 1 cut(s) | 312 |
| BstV1I | GCAGC | 7 cut(s) | 176, 361, 364, 385, 646, 1156, 1213 |
| BstV2I | GAAGAC | 3 cut(s) | 511, 518, 689 |
| BstX2I | RGATCY | 1 cut(s) | 1274 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 329 |
| BstYI | RGATCY | 1 cut(s) | 1274 |
| BstZ17I | GTATAC | 1 cut(s) | 230 |
| BsuRI | GGCC | 3 cut(s) | 182, 213, 1067 |
| BtgI | CCRYGG | 1 cut(s) | 120 |
| BtgZI | GCGATG | 1 cut(s) | 780 |
| BtsCI | GGATG | 7 cut(s) | 529, 560, 850, 1105, 1141, 1210, 1238 |
| BtsIMutI | CAGTG | 2 cut(s) | 542, 568 |
| BveI | ACCTGC | 2 cut(s) | 298, 478 |
| Cac8I | GCNNGC | 3 cut(s) | 19, 23, 1206 |
| Cfr13I | GGNCC | 4 cut(s) | 181, 1065, 1160, 1304 |
| CseI | GACGC | 1 cut(s) | 287 |
| CsiI | ACCWGGT | 1 cut(s) | 265 |
| Csp6I | GTAC | 5 cut(s) | 145, 577, 588, 833, 1269 |
| CviAII | CATG | 6 cut(s) | 18, 121, 323, 688, 1132, 1197 |
| CviQI | GTAC | 5 cut(s) | 145, 577, 588, 833, 1269 |
| DdeI | CTNAG | 4 cut(s) | 78, 86, 677, 1009 |
| DpnI | GATC | 5 cut(s) | 447, 455, 1035, 1130, 1276 |
| DpnII | GATC | 5 cut(s) | 445, 453, 1033, 1128, 1274 |
| DraIII | CACNNNGTG | 1 cut(s) | 539 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1166 |
| Eam1104I | CTCTTC | 3 cut(s) | 77, 868, 1267 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1166 |
| EarI | CTCTTC | 3 cut(s) | 77, 868, 1267 |
| Ecl136II | GAGCTC | 1 cut(s) | 11 |
| Eco130I | CCWWGG | 1 cut(s) | 120 |
| Eco147I | AGGCCT | 1 cut(s) | 213 |
| Eco24I | GRGCYC | 1 cut(s) | 13 |
| Eco47I | GGWCC | 2 cut(s) | 1160, 1304 |
| Eco53kI | GAGCTC | 1 cut(s) | 11 |
| Eco57I | CTGAAG | 2 cut(s) | 505, 1268 |
| Eco88I | CYCGRG | 1 cut(s) | 241 |
| Eco91I | GGTNACC | 1 cut(s) | 95 |
| EcoICRI | GAGCTC | 1 cut(s) | 11 |
| EcoO65I | GGTNACC | 1 cut(s) | 95 |
| EcoRII | CCWGG | 1 cut(s) | 265 |
| EcoT14I | CCWWGG | 1 cut(s) | 120 |
| EcoT22I | ATGCAT | 1 cut(s) | 799 |
| EcoT38I | GRGCYC | 1 cut(s) | 13 |
| ErhI | CCWWGG | 1 cut(s) | 120 |
| FaeI | CATG | 6 cut(s) | 21, 124, 326, 691, 1135, 1200 |
| FaqI | GGGAC | 4 cut(s) | 259, 755, 875, 1234 |
| FatI | CATG | 6 cut(s) | 17, 120, 322, 687, 1131, 1196 |
| FblI | GTMKAC | 2 cut(s) | 229, 983 |
| Fnu4HI | GCNGC | 8 cut(s) | 165, 180, 375, 378, 399, 660, 1145, 1202 |
| FokI | GGATG | 7 cut(s) | 516, 567, 857, 1112, 1148, 1197, 1245 |
| FriOI | GRGCYC | 1 cut(s) | 13 |
| Fsp4HI | GCNGC | 8 cut(s) | 165, 180, 375, 378, 399, 660, 1145, 1202 |
| FspBI | CTAG | 4 cut(s) | 14, 1079, 1085, 1272 |
| GluI | GCNGC | 8 cut(s) | 165, 180, 375, 378, 399, 660, 1145, 1202 |
| GsuI | CTGGAG | 2 cut(s) | 1176, 1302 |
| HaeIII | GGCC | 3 cut(s) | 182, 213, 1067 |
| HapII | CCGG | 2 cut(s) | 60, 459 |
| HgaI | GACGC | 1 cut(s) | 287 |
| Hin1II | CATG | 6 cut(s) | 21, 124, 326, 691, 1135, 1200 |
| HincII | GTYRAC | 1 cut(s) | 927 |
| HindII | GTYRAC | 1 cut(s) | 927 |
| HindIII | AAGCTT | 1 cut(s) | 283 |
| HinfI | GANTC | 6 cut(s) | 345, 595, 743, 759, 908, 1018 |
| HpaI | GTTAAC | 1 cut(s) | 927 |
| HpaII | CCGG | 2 cut(s) | 60, 459 |
| HphI | GGTGA | 1 cut(s) | 289 |
| Hpy166II | GTNNAC | 5 cut(s) | 43, 157, 230, 927, 984 |
| Hpy188I | TCNGA | 5 cut(s) | 81, 344, 450, 550, 920 |
| Hpy188III | TCNNGA | 4 cut(s) | 459, 724, 1085, 1155 |
| Hpy8I | GTNNAC | 5 cut(s) | 43, 157, 230, 927, 984 |
| Hpy99I | CGWCG | 2 cut(s) | 303, 306 |
| HpyAV | CCTTC | 5 cut(s) | 545, 634, 636, 680, 1243 |
| HpyCH4III | ACNGT | 3 cut(s) | 537, 563, 778 |
| HpyCH4IV | ACGT | 2 cut(s) | 248, 1005 |
| HpyCH4V | TGCA | 9 cut(s) | 25, 43, 260, 335, 662, 797, 1144, 1196, 1226 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 407, 1064, 1186 |
| HpyF3I | CTNAG | 4 cut(s) | 78, 86, 677, 1009 |
| HpySE526I | ACGT | 2 cut(s) | 248, 1005 |
| Hsp92II | CATG | 6 cut(s) | 21, 124, 326, 691, 1135, 1200 |
| Kpn2I | TCCGGA | 1 cut(s) | 458 |
| KspAI | GTTAAC | 1 cut(s) | 927 |
| Kzo9I | GATC | 5 cut(s) | 445, 453, 1033, 1128, 1274 |
| LmnI | GCTCC | 1 cut(s) | 434 |
| Lsp1109I | GCAGC | 7 cut(s) | 176, 361, 364, 385, 646, 1156, 1213 |
| LweI | GCATC | 4 cut(s) | 545, 757, 1126, 1223 |
| MabI | ACCWGGT | 1 cut(s) | 265 |
| MaeI | CTAG | 4 cut(s) | 14, 1079, 1085, 1272 |
| MaeII | ACGT | 2 cut(s) | 248, 1005 |
| MaeIII | GTNAC | 4 cut(s) | 95, 494, 1110, 1337 |
| MalI | GATC | 5 cut(s) | 447, 455, 1035, 1130, 1276 |
| MboI | GATC | 5 cut(s) | 445, 453, 1033, 1128, 1274 |
| MboII | GAAGA | 8 cut(s) | 64, 511, 518, 694, 885, 1043, 1254, 1343 |
| MflI | RGATCY | 1 cut(s) | 1274 |
| MhlI | GDGCHC | 3 cut(s) | 13, 45, 618 |
| MluCI | AATT | 8 cut(s) | 255, 414, 429, 441, 605, 727, 784, 948 |
| MlyI | GAGTC | 2 cut(s) | 737, 768 |
| MnlI | CCTC | 9 cut(s) | 80, 194, 203, 834, 903, 1151, 1222, 1247, 1285 |
| Mph1103I | ATGCAT | 1 cut(s) | 799 |
| MroI | TCCGGA | 1 cut(s) | 458 |
| MseI | TTAA | 9 cut(s) | 54, 218, 254, 750, 783, 926, 951, 1116, 1149 |
| MspI | CCGG | 2 cut(s) | 60, 459 |
| MspR9I | CCNGG | 2 cut(s) | 60, 267 |
| Mva1269I | GAATGC | 1 cut(s) | 236 |
| MvaI | CCWGG | 1 cut(s) | 267 |
| MvnI | CGCG | 1 cut(s) | 312 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 407, 1064, 1186 |
| NciI | CCSGG | 1 cut(s) | 60 |
| NcoI | CCATGG | 1 cut(s) | 120 |
| NdeII | GATC | 5 cut(s) | 445, 453, 1033, 1128, 1274 |
| NlaIII | CATG | 6 cut(s) | 21, 124, 326, 691, 1135, 1200 |
| NlaIV | GGNNCC | 1 cut(s) | 1306 |
| NmuCI | GTSAC | 2 cut(s) | 494, 1337 |
| NsiI | ATGCAT | 1 cut(s) | 799 |
| NspI | RCATGY | 1 cut(s) | 21 |
| PaeI | GCATGC | 1 cut(s) | 21 |
| PceI | AGGCCT | 1 cut(s) | 213 |
| PctI | GAATGC | 1 cut(s) | 236 |
| PfeI | GAWTC | 4 cut(s) | 345, 595, 908, 1018 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 126 |
| PkrI | GCNGC | 8 cut(s) | 166, 181, 376, 379, 400, 661, 1146, 1203 |
| PleI | GAGTC | 2 cut(s) | 737, 767 |
| PpsI | GAGTC | 2 cut(s) | 737, 767 |
| PshBI | ATTAAT | 1 cut(s) | 783 |
| Psp124BI | GAGCTC | 1 cut(s) | 13 |
| Psp6I | CCWGG | 1 cut(s) | 265 |
| PspEI | GGTNACC | 1 cut(s) | 95 |
| PspGI | CCWGG | 1 cut(s) | 265 |
| PspN4I | GGNNCC | 1 cut(s) | 1306 |
| PspPI | GGNCC | 4 cut(s) | 181, 1065, 1160, 1304 |
| PstI | CTGCAG | 1 cut(s) | 1228 |
| PsuI | RGATCY | 1 cut(s) | 1274 |
| RsaI | GTAC | 5 cut(s) | 146, 578, 589, 834, 1270 |
| RsaNI | GTAC | 5 cut(s) | 145, 577, 588, 833, 1269 |
| SacI | GAGCTC | 1 cut(s) | 13 |
| SaqAI | TTAA | 9 cut(s) | 54, 218, 254, 750, 783, 926, 951, 1116, 1149 |
| SatI | GCNGC | 8 cut(s) | 165, 180, 375, 378, 399, 660, 1145, 1202 |
| Sau3AI | GATC | 5 cut(s) | 445, 453, 1033, 1128, 1274 |
| Sau96I | GGNCC | 4 cut(s) | 181, 1065, 1160, 1304 |
| ScaI | AGTACT | 1 cut(s) | 1270 |
| SchI | GAGTC | 2 cut(s) | 737, 768 |
| ScrFI | CCNGG | 2 cut(s) | 60, 267 |
| SduI | GDGCHC | 3 cut(s) | 13, 45, 618 |
| SexAI | ACCWGGT | 1 cut(s) | 265 |
| SfaNI | GCATC | 4 cut(s) | 545, 757, 1126, 1223 |
| SfcI | CTRYAG | 2 cut(s) | 222, 1224 |
| SinI | GGWCC | 2 cut(s) | 1160, 1304 |
| SmlI | CTYRAG | 1 cut(s) | 1238 |
| SmoI | CTYRAG | 1 cut(s) | 1238 |
| SphI | GCATGC | 1 cut(s) | 21 |
| Sse9I | AATT | 8 cut(s) | 255, 414, 429, 441, 605, 727, 784, 948 |
| SseBI | AGGCCT | 1 cut(s) | 213 |
| SsiI | CCGC | 2 cut(s) | 179, 310 |
| SspI | AATATT | 1 cut(s) | 202 |
| SspMI | CTAG | 4 cut(s) | 14, 1079, 1085, 1272 |
| SstI | GAGCTC | 1 cut(s) | 13 |
| StuI | AGGCCT | 1 cut(s) | 213 |
| StyD4I | CCNGG | 2 cut(s) | 58, 265 |
| StyI | CCWWGG | 1 cut(s) | 120 |
| TaaI | ACNGT | 3 cut(s) | 537, 563, 778 |
| TaiI | ACGT | 2 cut(s) | 251, 1008 |
| TaqI | TCGA | 2 cut(s) | 354, 902 |
| TasI | AATT | 8 cut(s) | 255, 414, 429, 441, 605, 727, 784, 948 |
| TatI | WGTACW | 2 cut(s) | 587, 1268 |
| TauI | GCSGC | 1 cut(s) | 182 |
| TfiI | GAWTC | 4 cut(s) | 345, 595, 908, 1018 |
| Tru1I | TTAA | 9 cut(s) | 54, 218, 254, 750, 783, 926, 951, 1116, 1149 |
| Tru9I | TTAA | 9 cut(s) | 54, 218, 254, 750, 783, 926, 951, 1116, 1149 |
| TscAI | CASTG | 2 cut(s) | 542, 568 |
| TseFI | GTSAC | 2 cut(s) | 494, 1337 |
| TseI | GCWGC | 7 cut(s) | 164, 374, 377, 398, 659, 1144, 1201 |
| Tsp45I | GTSAC | 2 cut(s) | 494, 1337 |
| TspDTI | ATGAA | 5 cut(s) | 337, 372, 402, 518, 981 |
| TspRI | CASTG | 2 cut(s) | 542, 568 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 126 |
| VneI | GTGCAC | 1 cut(s) | 41 |
| VpaK11BI | GGWCC | 2 cut(s) | 1160, 1304 |
| VspI | ATTAAT | 1 cut(s) | 783 |
| XbaI | TCTAGA | 1 cut(s) | 1084 |
| XceI | RCATGY | 1 cut(s) | 21 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 865 |
| XmiI | GTMKAC | 2 cut(s) | 229, 983 |
| XspI | CTAG | 4 cut(s) | 14, 1079, 1085, 1272 |
| ZrmI | AGTACT | 1 cut(s) | 1270 |
| Zsp2I | ATGCAT | 1 cut(s) | 799 |