Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 30 |
| Acc36I | ACCTGC | 1 cut(s) | 223 |
| AccI | GTMKAC | 1 cut(s) | 5 |
| AcuI | CTGAAG | 1 cut(s) | 290 |
| AcyI | GRCGYC | 1 cut(s) | 27 |
| AfaI | GTAC | 1 cut(s) | 292 |
| AgsI | TTSAA | 1 cut(s) | 288 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 188 |
| AluBI | AGCT | 5 cut(s) | 65, 80, 169, 211, 226 |
| AluI | AGCT | 5 cut(s) | 65, 80, 169, 211, 226 |
| ApeKI | GCWGC | 2 cut(s) | 166, 223 |
| AspS9I | GGNCC | 3 cut(s) | 87, 182, 326 |
| AvaII | GGWCC | 3 cut(s) | 87, 182, 326 |
| BbvI | GCAGC | 2 cut(s) | 178, 235 |
| BfaI | CTAG | 3 cut(s) | 101, 107, 294 |
| BfmI | CTRYAG | 1 cut(s) | 246 |
| BfuAI | ACCTGC | 1 cut(s) | 223 |
| BglII | AGATCT | 1 cut(s) | 296 |
| BisI | GCNGC | 2 cut(s) | 167, 224 |
| BlsI | GCNGC | 2 cut(s) | 168, 225 |
| BmcAI | AGTACT | 1 cut(s) | 292 |
| Bme18I | GGWCC | 3 cut(s) | 87, 182, 326 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 188 |
| BmgT120I | GGNCC | 3 cut(s) | 87, 182, 326 |
| BmiI | GGNNCC | 1 cut(s) | 328 |
| BmsI | GCATC | 1 cut(s) | 148 |
| BpmI | CTGGAG | 2 cut(s) | 198, 324 |
| BpuEI | CTTGAG | 1 cut(s) | 245 |
| BsaHI | GRCGYC | 1 cut(s) | 27 |
| BseGI | GGATG | 3 cut(s) | 127, 163, 232 |
| BseXI | GCAGC | 2 cut(s) | 178, 235 |
| BslFI | GGGAC | 1 cut(s) | 256 |
| BsmFI | GGGAC | 1 cut(s) | 256 |
| BsmI | GAATGC | 1 cut(s) | 260 |
| Bsp143I | GATC | 3 cut(s) | 150, 296, 355 |
| BspLI | GGNNCC | 1 cut(s) | 328 |
| BspMAI | CTGCAG | 1 cut(s) | 250 |
| BspMI | ACCTGC | 1 cut(s) | 223 |
| BssMI | GATC | 3 cut(s) | 150, 296, 355 |
| BssNI | GRCGYC | 1 cut(s) | 27 |
| Bst6I | CTCTTC | 1 cut(s) | 289 |
| BstACI | GRCGYC | 1 cut(s) | 27 |
| BstC8I | GCNNGC | 1 cut(s) | 228 |
| BstF5I | GGATG | 3 cut(s) | 127, 163, 232 |
| BstKTI | GATC | 3 cut(s) | 153, 299, 358 |
| BstMBI | GATC | 3 cut(s) | 150, 296, 355 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 208 |
| BstSFI | CTRYAG | 1 cut(s) | 246 |
| BstV1I | GCAGC | 2 cut(s) | 178, 235 |
| BstX2I | RGATCY | 1 cut(s) | 296 |
| BstYI | RGATCY | 1 cut(s) | 296 |
| BtsCI | GGATG | 3 cut(s) | 127, 163, 232 |
| BveI | ACCTGC | 1 cut(s) | 223 |
| Cac8I | GCNNGC | 1 cut(s) | 228 |
| Cfr13I | GGNCC | 3 cut(s) | 87, 182, 326 |
| Csp6I | GTAC | 1 cut(s) | 291 |
| CviAII | CATG | 2 cut(s) | 154, 219 |
| CviJI | RGCY | 7 cut(s) | 65, 80, 130, 169, 211, 226, 268 |
| CviKI_1 | RGCY | 7 cut(s) | 65, 80, 130, 169, 211, 226, 268 |
| CviQI | GTAC | 1 cut(s) | 291 |
| DpnI | GATC | 3 cut(s) | 152, 298, 357 |
| DpnII | GATC | 3 cut(s) | 150, 296, 355 |
| DriI | GACNNNNNGTC | 1 cut(s) | 188 |
| Eam1104I | CTCTTC | 1 cut(s) | 289 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 188 |
| EarI | CTCTTC | 1 cut(s) | 289 |
| Eco47I | GGWCC | 3 cut(s) | 87, 182, 326 |
| Eco57I | CTGAAG | 1 cut(s) | 290 |
| FaeI | CATG | 2 cut(s) | 157, 222 |
| FaiI | YATR | 5 cut(s) | 16, 92, 155, 220, 311 |
| FaqI | GGGAC | 1 cut(s) | 256 |
| FatI | CATG | 2 cut(s) | 153, 218 |
| FblI | GTMKAC | 1 cut(s) | 5 |
| Fnu4HI | GCNGC | 2 cut(s) | 167, 224 |
| FokI | GGATG | 3 cut(s) | 134, 170, 219 |
| Fsp4HI | GCNGC | 2 cut(s) | 167, 224 |
| FspBI | CTAG | 3 cut(s) | 101, 107, 294 |
| GluI | GCNGC | 2 cut(s) | 167, 224 |
| GsuI | CTGGAG | 2 cut(s) | 198, 324 |
| Hin1I | GRCGYC | 1 cut(s) | 27 |
| Hin1II | CATG | 2 cut(s) | 157, 222 |
| HinfI | GANTC | 1 cut(s) | 40 |
| Hpy166II | GTNNAC | 1 cut(s) | 6 |
| Hpy188III | TCNNGA | 3 cut(s) | 107, 177, 359 |
| Hpy8I | GTNNAC | 1 cut(s) | 6 |
| Hpy99I | CGWCG | 1 cut(s) | 29 |
| HpyAV | CCTTC | 1 cut(s) | 265 |
| HpyCH4IV | ACGT | 1 cut(s) | 27 |
| HpyCH4V | TGCA | 4 cut(s) | 60, 166, 218, 248 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 208 |
| HpySE526I | ACGT | 1 cut(s) | 27 |
| Hsp92I | GRCGYC | 1 cut(s) | 27 |
| Hsp92II | CATG | 2 cut(s) | 157, 222 |
| Kzo9I | GATC | 3 cut(s) | 150, 296, 355 |
| LpnPI | CCDG | 5 cut(s) | 63, 103, 162, 228, 288 |
| Lsp1109I | GCAGC | 2 cut(s) | 178, 235 |
| LweI | GCATC | 1 cut(s) | 148 |
| MaeI | CTAG | 3 cut(s) | 101, 107, 294 |
| MaeII | ACGT | 1 cut(s) | 27 |
| MaeIII | GTNAC | 1 cut(s) | 359 |
| MalI | GATC | 3 cut(s) | 152, 298, 357 |
| MboI | GATC | 3 cut(s) | 150, 296, 355 |
| MboII | GAAGA | 3 cut(s) | 65, 276, 365 |
| MflI | RGATCY | 1 cut(s) | 296 |
| MnlI | CCTC | 4 cut(s) | 173, 244, 269, 307 |
| MseI | TTAA | 3 cut(s) | 32, 138, 171 |
| Mva1269I | GAATGC | 1 cut(s) | 260 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 208 |
| NdeII | GATC | 3 cut(s) | 150, 296, 355 |
| NlaIII | CATG | 2 cut(s) | 157, 222 |
| NlaIV | GGNNCC | 1 cut(s) | 328 |
| NmuCI | GTSAC | 1 cut(s) | 359 |
| PctI | GAATGC | 1 cut(s) | 260 |
| PfeI | GAWTC | 1 cut(s) | 40 |
| PkrI | GCNGC | 2 cut(s) | 168, 225 |
| PspN4I | GGNNCC | 1 cut(s) | 328 |
| PspPI | GGNCC | 3 cut(s) | 87, 182, 326 |
| PstI | CTGCAG | 1 cut(s) | 250 |
| PsuI | RGATCY | 1 cut(s) | 296 |
| RsaI | GTAC | 1 cut(s) | 292 |
| RsaNI | GTAC | 1 cut(s) | 291 |
| SaqAI | TTAA | 3 cut(s) | 32, 138, 171 |
| SatI | GCNGC | 2 cut(s) | 167, 224 |
| Sau3AI | GATC | 3 cut(s) | 150, 296, 355 |
| Sau96I | GGNCC | 3 cut(s) | 87, 182, 326 |
| ScaI | AGTACT | 1 cut(s) | 292 |
| SetI | ASST | 9 cut(s) | 30, 67, 82, 89, 106, 171, 213, 217, 228 |
| SfaNI | GCATC | 1 cut(s) | 148 |
| SfcI | CTRYAG | 1 cut(s) | 246 |
| SinI | GGWCC | 3 cut(s) | 87, 182, 326 |
| SmlI | CTYRAG | 1 cut(s) | 260 |
| SmoI | CTYRAG | 1 cut(s) | 260 |
| SspMI | CTAG | 3 cut(s) | 101, 107, 294 |
| TaiI | ACGT | 1 cut(s) | 30 |
| TatI | WGTACW | 1 cut(s) | 290 |
| TfiI | GAWTC | 1 cut(s) | 40 |
| Tru1I | TTAA | 3 cut(s) | 32, 138, 171 |
| Tru9I | TTAA | 3 cut(s) | 32, 138, 171 |
| TseFI | GTSAC | 1 cut(s) | 359 |
| TseI | GCWGC | 2 cut(s) | 166, 223 |
| Tsp45I | GTSAC | 1 cut(s) | 359 |
| VpaK11BI | GGWCC | 3 cut(s) | 87, 182, 326 |
| XbaI | TCTAGA | 1 cut(s) | 106 |
| XmiI | GTMKAC | 1 cut(s) | 5 |
| XspI | CTAG | 3 cut(s) | 101, 107, 294 |
| ZraI | GACGTC | 1 cut(s) | 28 |
| ZrmI | AGTACT | 1 cut(s) | 292 |