Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 3 cut(s) | 317, 601, 1272 |
| AccI | GTMKAC | 2 cut(s) | 5, 580 |
| AccII | CGCG | 1 cut(s) | 117 |
| AccIII | TCCGGA | 1 cut(s) | 591 |
| AciI | CCGC | 1 cut(s) | 815 |
| AclWI | GGATC | 7 cut(s) | 68, 441, 560, 573, 1101, 1114, 1271 |
| AcsI | RAATTY | 1 cut(s) | 398 |
| AcuI | CTGAAG | 3 cut(s) | 775, 782, 1155 |
| AfaI | GTAC | 3 cut(s) | 227, 965, 1467 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 106, 129, 575, 824, 877, 884, 1103 |
| AflII | CTTAAG | 1 cut(s) | 1022 |
| AflIII | ACRYGT | 1 cut(s) | 960 |
| AgsI | TTSAA | 8 cut(s) | 38, 514, 772, 1127, 1185, 1334, 1420, 1542 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1442 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1107, 1139 |
| Alw26I | GTCTC | 2 cut(s) | 545, 1177 |
| AlwI | GGATC | 7 cut(s) | 68, 441, 560, 573, 1101, 1114, 1271 |
| Ama87I | CYCGRG | 1 cut(s) | 744 |
| Aor13HI | TCCGGA | 1 cut(s) | 591 |
| AoxI | GGCC | 2 cut(s) | 1338, 1357 |
| ApeKI | GCWGC | 6 cut(s) | 73, 644, 647, 668, 932, 1477 |
| ApoI | RAATTY | 1 cut(s) | 398 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 383, 415 |
| AspLEI | GCGC | 4 cut(s) | 318, 602, 891, 1273 |
| AspS9I | GGNCC | 4 cut(s) | 133, 489, 1338, 1436 |
| AsuC2I | CCSGG | 2 cut(s) | 137, 1495 |
| AsuHPI | GGTGA | 3 cut(s) | 80, 254, 1009 |
| AsuNHI | GCTAGC | 1 cut(s) | 1480 |
| AvaI | CYCGRG | 1 cut(s) | 744 |
| AvaII | GGWCC | 3 cut(s) | 133, 489, 1436 |
| BamHI | GGATCC | 2 cut(s) | 565, 1106 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 947, 979 |
| BbsI | GAAGAC | 2 cut(s) | 781, 788 |
| BbvI | GCAGC | 6 cut(s) | 60, 631, 634, 655, 919, 1489 |
| BccI | CCATC | 6 cut(s) | 123, 449, 605, 826, 1388, 1519 |
| BceAI | ACGGC | 1 cut(s) | 321 |
| BcnI | CCSGG | 2 cut(s) | 137, 1495 |
| BcoDI | GTCTC | 2 cut(s) | 545, 1177 |
| BfaI | CTAG | 5 cut(s) | 41, 387, 623, 1355, 1481 |
| BfmI | CTRYAG | 2 cut(s) | 865, 1500 |
| BfrI | CTTAAG | 1 cut(s) | 1022 |
| BisI | GCNGC | 7 cut(s) | 74, 645, 648, 669, 815, 933, 1478 |
| BlsI | GCNGC | 7 cut(s) | 75, 646, 649, 670, 816, 934, 1479 |
| BmcAI | AGTACT | 1 cut(s) | 1467 |
| Bme1390I | CCNGG | 2 cut(s) | 137, 1495 |
| Bme18I | GGWCC | 3 cut(s) | 133, 489, 1436 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1442 |
| BmeT110I | CYCGRG | 1 cut(s) | 744 |
| BmgT120I | GGNCC | 4 cut(s) | 133, 489, 1338, 1436 |
| BmiI | GGNNCC | 3 cut(s) | 134, 567, 1108 |
| BmrFI | CCNGG | 2 cut(s) | 137, 1495 |
| BmsI | GCATC | 4 cut(s) | 303, 409, 818, 1030 |
| BmtI | GCTAGC | 1 cut(s) | 1484 |
| BoxI | GACNNNNGTC | 1 cut(s) | 375 |
| BpiI | GAAGAC | 2 cut(s) | 781, 788 |
| BpmI | CTGGAG | 1 cut(s) | 1452 |
| BpuEI | CTTGAG | 2 cut(s) | 86, 237 |
| BpuMI | CCSGG | 2 cut(s) | 137, 1495 |
| BsaI | GGTCTC | 1 cut(s) | 545 |
| BsaJI | CCNNGG | 1 cut(s) | 237 |
| BsaWI | WCCGGW | 3 cut(s) | 409, 591, 877 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 106, 129, 575, 824, 877, 884, 1103 |
| Bse118I | RCCGGY | 2 cut(s) | 122, 194 |
| Bse1I | ACTGG | 1 cut(s) | 1439 |
| Bse3DI | GCAATG | 3 cut(s) | 68, 174, 429 |
| BseAI | TCCGGA | 1 cut(s) | 591 |
| BseDI | CCNNGG | 1 cut(s) | 237 |
| BseGI | GGATG | 9 cut(s) | 134, 318, 736, 790, 833, 976, 1381, 1486, 1527 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 106, 129, 575, 824, 877, 884, 1103 |
| BseMI | GCAATG | 3 cut(s) | 68, 174, 429 |
| BseMII | CTCAG | 3 cut(s) | 42, 201, 818 |
| BseNI | ACTGG | 1 cut(s) | 1439 |
| BseRI | GAGGAG | 3 cut(s) | 68, 442, 525 |
| BseXI | GCAGC | 6 cut(s) | 60, 631, 634, 655, 919, 1489 |
| BseYI | CCCAGC | 1 cut(s) | 11 |
| Bsh1236I | CGCG | 1 cut(s) | 117 |
| BshFI | GGCC | 2 cut(s) | 1340, 1359 |
| BsiHKCI | CYCGRG | 1 cut(s) | 744 |
| BsiSI | CCGG | 7 cut(s) | 123, 136, 195, 410, 592, 878, 1494 |
| BslFI | GGGAC | 3 cut(s) | 41, 165, 1510 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 106, 129, 575, 824, 877, 884, 1103 |
| BsmAI | GTCTC | 2 cut(s) | 545, 1177 |
| BsmFI | GGGAC | 3 cut(s) | 41, 165, 1510 |
| BsmI | GAATGC | 2 cut(s) | 381, 1514 |
| BsnI | GGCC | 2 cut(s) | 1340, 1359 |
| Bso31I | GGTCTC | 1 cut(s) | 545 |
| BsoBI | CYCGRG | 1 cut(s) | 744 |
| Bsp13I | TCCGGA | 1 cut(s) | 591 |
| Bsp1407I | TGTACA | 1 cut(s) | 963 |
| Bsp143I | GATC | 9 cut(s) | 60, 433, 565, 715, 723, 1106, 1276, 1306, 1351 |
| BspACI | CCGC | 1 cut(s) | 815 |
| BspANI | GGCC | 2 cut(s) | 1340, 1359 |
| BspCNI | CTCAG | 3 cut(s) | 43, 202, 817 |
| BspEI | TCCGGA | 1 cut(s) | 591 |
| BspFNI | CGCG | 1 cut(s) | 117 |
| BspHI | TCATGA | 1 cut(s) | 720 |
| BspLI | GGNNCC | 3 cut(s) | 134, 567, 1108 |
| BspMAI | CTGCAG | 1 cut(s) | 1504 |
| BspOI | GCTAGC | 1 cut(s) | 1484 |
| BspPI | GGATC | 7 cut(s) | 68, 441, 560, 573, 1101, 1114, 1271 |
| BspQI | GCTCTTC | 1 cut(s) | 1306 |
| BspTI | CTTAAG | 1 cut(s) | 1022 |
| BspTNI | GGTCTC | 1 cut(s) | 545 |
| BsrDI | GCAATG | 3 cut(s) | 68, 174, 429 |
| BsrFI | RCCGGY | 2 cut(s) | 122, 194 |
| BsrGI | TGTACA | 1 cut(s) | 963 |
| BsrI | ACTGG | 1 cut(s) | 1439 |
| BssAI | RCCGGY | 2 cut(s) | 122, 194 |
| BssECI | CCNNGG | 1 cut(s) | 237 |
| BssMI | GATC | 9 cut(s) | 60, 433, 565, 715, 723, 1106, 1276, 1306, 1351 |
| Bst4CI | ACNGT | 1 cut(s) | 377 |
| Bst6I | CTCTTC | 2 cut(s) | 354, 1306 |
| BstAFI | CTTAAG | 1 cut(s) | 1022 |
| BstAUI | TGTACA | 1 cut(s) | 963 |
| BstC8I | GCNNGC | 1 cut(s) | 1482 |
| BstDEI | CTNAG | 3 cut(s) | 51, 210, 804 |
| BstDSI | CCRYGG | 1 cut(s) | 237 |
| BstF5I | GGATG | 9 cut(s) | 134, 318, 736, 790, 833, 976, 1381, 1486, 1527 |
| BstFNI | CGCG | 1 cut(s) | 117 |
| BstHHI | GCGC | 4 cut(s) | 318, 602, 891, 1273 |
| BstKTI | GATC | 9 cut(s) | 63, 436, 568, 718, 726, 1109, 1279, 1309, 1354 |
| BstMAI | GTCTC | 2 cut(s) | 545, 1177 |
| BstMBI | GATC | 9 cut(s) | 60, 433, 565, 715, 723, 1106, 1276, 1306, 1351 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 674, 845 |
| BstNSI | RCATGY | 1 cut(s) | 964 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 375 |
| BstSCI | CCNGG | 2 cut(s) | 135, 1493 |
| BstSFI | CTRYAG | 2 cut(s) | 865, 1500 |
| BstUI | CGCG | 1 cut(s) | 117 |
| BstV1I | GCAGC | 6 cut(s) | 60, 631, 634, 655, 919, 1489 |
| BstV2I | GAAGAC | 2 cut(s) | 781, 788 |
| BstX2I | RGATCY | 5 cut(s) | 60, 433, 565, 1106, 1276 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 401 |
| BstYI | RGATCY | 5 cut(s) | 60, 433, 565, 1106, 1276 |
| BsuRI | GGCC | 2 cut(s) | 1340, 1359 |
| BtgI | CCRYGG | 1 cut(s) | 237 |
| BtsCI | GGATG | 9 cut(s) | 134, 318, 736, 790, 833, 976, 1381, 1486, 1527 |
| BtsIMutI | CAGTG | 2 cut(s) | 812, 1042 |
| Cac8I | GCNNGC | 1 cut(s) | 1482 |
| CciI | TCATGA | 1 cut(s) | 720 |
| CfoI | GCGC | 4 cut(s) | 318, 602, 891, 1273 |
| Cfr10I | RCCGGY | 2 cut(s) | 122, 194 |
| Cfr13I | GGNCC | 4 cut(s) | 133, 489, 1338, 1436 |
| CseI | GACGC | 1 cut(s) | 106 |
| Csp6I | GTAC | 3 cut(s) | 226, 964, 1466 |
| CviAII | CATG | 5 cut(s) | 229, 721, 961, 1261, 1473 |
| CviQI | GTAC | 3 cut(s) | 226, 964, 1466 |
| DdeI | CTNAG | 3 cut(s) | 51, 210, 804 |
| DpnI | GATC | 9 cut(s) | 62, 435, 567, 717, 725, 1108, 1278, 1308, 1353 |
| DpnII | GATC | 9 cut(s) | 60, 433, 565, 715, 723, 1106, 1276, 1306, 1351 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1442 |
| Eam1104I | CTCTTC | 2 cut(s) | 354, 1306 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1442 |
| EarI | CTCTTC | 2 cut(s) | 354, 1306 |
| Eco147I | AGGCCT | 1 cut(s) | 1359 |
| Eco31I | GGTCTC | 1 cut(s) | 545 |
| Eco32I | GATATC | 1 cut(s) | 760 |
| Eco47I | GGWCC | 3 cut(s) | 133, 489, 1436 |
| Eco57I | CTGAAG | 3 cut(s) | 775, 782, 1155 |
| Eco88I | CYCGRG | 1 cut(s) | 744 |
| EcoRV | GATATC | 1 cut(s) | 760 |
| EcoT22I | ATGCAT | 2 cut(s) | 1072, 1514 |
| FaeI | CATG | 5 cut(s) | 232, 724, 964, 1264, 1476 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 907, 939 |
| FaqI | GGGAC | 3 cut(s) | 41, 165, 1510 |
| FatI | CATG | 5 cut(s) | 228, 720, 960, 1260, 1472 |
| FblI | GTMKAC | 2 cut(s) | 5, 580 |
| Fnu4HI | GCNGC | 7 cut(s) | 74, 645, 648, 669, 815, 933, 1478 |
| FokI | GGATG | 9 cut(s) | 141, 325, 723, 797, 840, 983, 1388, 1473, 1534 |
| Fsp4HI | GCNGC | 7 cut(s) | 74, 645, 648, 669, 815, 933, 1478 |
| FspAI | RTGCGCAY | 1 cut(s) | 317 |
| FspBI | CTAG | 5 cut(s) | 41, 387, 623, 1355, 1481 |
| FspI | TGCGCA | 3 cut(s) | 317, 601, 1272 |
| GlaI | GCGC | 4 cut(s) | 317, 601, 890, 1272 |
| GluI | GCNGC | 7 cut(s) | 74, 645, 648, 669, 815, 933, 1478 |
| GsaI | CCCAGC | 1 cut(s) | 15 |
| GsuI | CTGGAG | 1 cut(s) | 1452 |
| HaeIII | GGCC | 2 cut(s) | 1340, 1359 |
| HapII | CCGG | 7 cut(s) | 123, 136, 195, 410, 592, 878, 1494 |
| HgaI | GACGC | 1 cut(s) | 106 |
| HhaI | GCGC | 4 cut(s) | 318, 602, 891, 1273 |
| Hin1II | CATG | 5 cut(s) | 232, 724, 964, 1264, 1476 |
| Hin6I | GCGC | 4 cut(s) | 316, 600, 889, 1271 |
| HinP1I | GCGC | 4 cut(s) | 316, 600, 889, 1271 |
| HindIII | AAGCTT | 2 cut(s) | 143, 1421 |
| HinfI | GANTC | 5 cut(s) | 85, 532, 615, 699, 1190 |
| HpaII | CCGG | 7 cut(s) | 123, 136, 195, 410, 592, 878, 1494 |
| HphI | GGTGA | 3 cut(s) | 80, 254, 1009 |
| Hpy166II | GTNNAC | 3 cut(s) | 6, 346, 581 |
| Hpy188I | TCNGA | 5 cut(s) | 438, 704, 823, 1013, 1202 |
| Hpy188III | TCNNGA | 5 cut(s) | 103, 254, 592, 721, 1431 |
| Hpy8I | GTNNAC | 3 cut(s) | 6, 346, 581 |
| Hpy99I | CGWCG | 1 cut(s) | 122 |
| HpyAV | CCTTC | 5 cut(s) | 623, 748, 818, 909, 1500 |
| HpyCH4III | ACNGT | 1 cut(s) | 377 |
| HpyCH4IV | ACGT | 1 cut(s) | 352 |
| HpyCH4V | TGCA | 7 cut(s) | 162, 422, 671, 1070, 1472, 1502, 1512 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 674, 845 |
| HpyF3I | CTNAG | 3 cut(s) | 51, 210, 804 |
| HpySE526I | ACGT | 1 cut(s) | 352 |
| Hsp92II | CATG | 5 cut(s) | 232, 724, 964, 1264, 1476 |
| HspAI | GCGC | 4 cut(s) | 316, 600, 889, 1271 |
| Kpn2I | TCCGGA | 1 cut(s) | 591 |
| Kzo9I | GATC | 9 cut(s) | 60, 433, 565, 715, 723, 1106, 1276, 1306, 1351 |
| LguI | GCTCTTC | 1 cut(s) | 1306 |
| LmnI | GCTCC | 3 cut(s) | 109, 704, 1115 |
| Lsp1109I | GCAGC | 6 cut(s) | 60, 631, 634, 655, 919, 1489 |
| LweI | GCATC | 4 cut(s) | 303, 409, 818, 1030 |
| MaeI | CTAG | 5 cut(s) | 41, 387, 623, 1355, 1481 |
| MaeII | ACGT | 1 cut(s) | 352 |
| MaeIII | GTNAC | 6 cut(s) | 95, 368, 389, 529, 764, 1177 |
| MalI | GATC | 9 cut(s) | 62, 435, 567, 717, 725, 1108, 1278, 1308, 1353 |
| MboI | GATC | 9 cut(s) | 60, 433, 565, 715, 723, 1106, 1276, 1306, 1351 |
| MboII | GAAGA | 9 cut(s) | 55, 371, 781, 788, 967, 1069, 1148, 1323, 1530 |
| MflI | RGATCY | 5 cut(s) | 60, 433, 565, 1106, 1276 |
| MlyI | GAGTC | 1 cut(s) | 526 |
| MmeI | TCCRAC | 1 cut(s) | 540 |
| Mph1103I | ATGCAT | 2 cut(s) | 1072, 1514 |
| MroI | TCCGGA | 1 cut(s) | 591 |
| MseI | TTAA | 5 cut(s) | 465, 1023, 1233, 1425, 1552 |
| MspCI | CTTAAG | 1 cut(s) | 1022 |
| MspI | CCGG | 7 cut(s) | 123, 136, 195, 410, 592, 878, 1494 |
| MspR9I | CCNGG | 2 cut(s) | 137, 1495 |
| Mva1269I | GAATGC | 2 cut(s) | 381, 1514 |
| MvnI | CGCG | 1 cut(s) | 117 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 674, 845 |
| NciI | CCSGG | 2 cut(s) | 137, 1495 |
| NdeII | GATC | 9 cut(s) | 60, 433, 565, 715, 723, 1106, 1276, 1306, 1351 |
| NheI | GCTAGC | 1 cut(s) | 1480 |
| NlaIII | CATG | 5 cut(s) | 232, 724, 964, 1264, 1476 |
| NlaIV | GGNNCC | 3 cut(s) | 134, 567, 1108 |
| NmuCI | GTSAC | 3 cut(s) | 368, 389, 529 |
| NsbI | TGCGCA | 3 cut(s) | 317, 601, 1272 |
| NsiI | ATGCAT | 2 cut(s) | 1072, 1514 |
| NspI | RCATGY | 1 cut(s) | 964 |
| PagI | TCATGA | 1 cut(s) | 720 |
| PceI | AGGCCT | 1 cut(s) | 1359 |
| PciI | ACATGT | 1 cut(s) | 960 |
| PciSI | GCTCTTC | 1 cut(s) | 1306 |
| PctI | GAATGC | 2 cut(s) | 381, 1514 |
| PfeI | GAWTC | 4 cut(s) | 85, 615, 699, 1190 |
| PflFI | GACNNNGTC | 1 cut(s) | 1367 |
| PfoI | TCCNGGA | 2 cut(s) | 135, 1493 |
| PkrI | GCNGC | 7 cut(s) | 75, 646, 649, 670, 816, 934, 1479 |
| PleI | GAGTC | 1 cut(s) | 526 |
| PpsI | GAGTC | 1 cut(s) | 526 |
| PscI | ACATGT | 1 cut(s) | 960 |
| PshAI | GACNNNNGTC | 1 cut(s) | 375 |
| PspFI | CCCAGC | 1 cut(s) | 11 |
| PspN4I | GGNNCC | 3 cut(s) | 134, 567, 1108 |
| PspPI | GGNCC | 4 cut(s) | 133, 489, 1338, 1436 |
| PstI | CTGCAG | 1 cut(s) | 1504 |
| PsuI | RGATCY | 5 cut(s) | 60, 433, 565, 1106, 1276 |
| PsyI | GACNNNGTC | 1 cut(s) | 1367 |
| RsaI | GTAC | 3 cut(s) | 227, 965, 1467 |
| RsaNI | GTAC | 3 cut(s) | 226, 964, 1466 |
| SapI | GCTCTTC | 1 cut(s) | 1306 |
| SaqAI | TTAA | 5 cut(s) | 465, 1023, 1233, 1425, 1552 |
| SatI | GCNGC | 7 cut(s) | 74, 645, 648, 669, 815, 933, 1478 |
| Sau3AI | GATC | 9 cut(s) | 60, 433, 565, 715, 723, 1106, 1276, 1306, 1351 |
| Sau96I | GGNCC | 4 cut(s) | 133, 489, 1338, 1436 |
| ScaI | AGTACT | 1 cut(s) | 1467 |
| SchI | GAGTC | 1 cut(s) | 526 |
| ScrFI | CCNGG | 2 cut(s) | 137, 1495 |
| SfaNI | GCATC | 4 cut(s) | 303, 409, 818, 1030 |
| SfcI | CTRYAG | 2 cut(s) | 865, 1500 |
| SgrAI | CRCCGGYG | 1 cut(s) | 122 |
| SinI | GGWCC | 3 cut(s) | 133, 489, 1436 |
| SmlI | CTYRAG | 3 cut(s) | 101, 252, 1022 |
| SmoI | CTYRAG | 3 cut(s) | 101, 252, 1022 |
| SseBI | AGGCCT | 1 cut(s) | 1359 |
| SsiI | CCGC | 1 cut(s) | 815 |
| SspMI | CTAG | 5 cut(s) | 41, 387, 623, 1355, 1481 |
| StuI | AGGCCT | 1 cut(s) | 1359 |
| StyD4I | CCNGG | 2 cut(s) | 135, 1493 |
| TaaI | ACNGT | 1 cut(s) | 377 |
| TaiI | ACGT | 1 cut(s) | 355 |
| TaqI | TCGA | 1 cut(s) | 1309 |
| TatI | WGTACW | 3 cut(s) | 225, 963, 1465 |
| TauI | GCSGC | 1 cut(s) | 817 |
| TfiI | GAWTC | 4 cut(s) | 85, 615, 699, 1190 |
| Tru1I | TTAA | 5 cut(s) | 465, 1023, 1233, 1425, 1552 |
| Tru9I | TTAA | 5 cut(s) | 465, 1023, 1233, 1425, 1552 |
| TscAI | CASTG | 2 cut(s) | 812, 1042 |
| TseFI | GTSAC | 3 cut(s) | 368, 389, 529 |
| TseI | GCWGC | 6 cut(s) | 73, 644, 647, 668, 932, 1477 |
| Tsp45I | GTSAC | 3 cut(s) | 368, 389, 529 |
| TspDTI | ATGAA | 7 cut(s) | 60, 607, 642, 672, 767, 921, 1070 |
| TspGWI | ACGGA | 2 cut(s) | 122, 226 |
| TspRI | CASTG | 2 cut(s) | 812, 1042 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1367 |
| Vha464I | CTTAAG | 1 cut(s) | 1022 |
| VpaK11BI | GGWCC | 3 cut(s) | 133, 489, 1436 |
| XapI | RAATTY | 1 cut(s) | 398 |
| XceI | RCATGY | 1 cut(s) | 964 |
| XmiI | GTMKAC | 2 cut(s) | 5, 580 |
| XspI | CTAG | 5 cut(s) | 41, 387, 623, 1355, 1481 |
| ZrmI | AGTACT | 1 cut(s) | 1467 |
| Zsp2I | ATGCAT | 2 cut(s) | 1072, 1514 |