Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PolyA_pol | PF01743 | 139 - 277 | 5.9e-31 | Poly A polymerase head domain |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 416 |
| AatII | GACGTC | 1 cut(s) | 1578 |
| Acc16I | TGCGCA | 1 cut(s) | 620 |
| Acc36I | ACCTGC | 1 cut(s) | 416 |
| AccI | GTMKAC | 2 cut(s) | 114, 236 |
| AccII | CGCG | 1 cut(s) | 1809 |
| AciI | CCGC | 6 cut(s) | 106, 682, 872, 895, 903, 1807 |
| AclWI | GGATC | 3 cut(s) | 744, 863, 876 |
| AcsI | RAATTY | 2 cut(s) | 35, 1588 |
| AcuI | CTGAAG | 4 cut(s) | 375, 1075, 1449, 1838 |
| AcyI | GRCGYC | 1 cut(s) | 1575 |
| AdeI | CACNNNGTG | 1 cut(s) | 1109 |
| AfaI | GTAC | 5 cut(s) | 321, 668, 1159, 1404, 1840 |
| AflII | CTTAAG | 2 cut(s) | 404, 1319 |
| AflIII | ACRYGT | 1 cut(s) | 322 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1736 |
| Alw26I | GTCTC | 2 cut(s) | 519, 1305 |
| AlwI | GGATC | 3 cut(s) | 744, 863, 876 |
| AoxI | GGCC | 1 cut(s) | 1635 |
| ApeKI | GCWGC | 8 cut(s) | 376, 501, 944, 947, 968, 1229, 1714, 1771 |
| ApoI | RAATTY | 2 cut(s) | 35, 1588 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 45, 77 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 552 |
| AspLEI | GCGC | 1 cut(s) | 621 |
| AspS9I | GGNCC | 4 cut(s) | 267, 436, 1730, 1874 |
| AsuII | TTCGAA | 2 cut(s) | 139, 1472 |
| AvaII | GGWCC | 4 cut(s) | 267, 436, 1730, 1874 |
| AxyI | CCTNAGG | 1 cut(s) | 129 |
| BaeGI | GKGCMC | 1 cut(s) | 1188 |
| BamHI | GGATCC | 1 cut(s) | 868 |
| BanII | GRGCYC | 2 cut(s) | 79, 193 |
| BbsI | GAAGAC | 3 cut(s) | 1081, 1088, 1259 |
| BbvI | GCAGC | 8 cut(s) | 363, 513, 931, 934, 955, 1216, 1726, 1783 |
| BccI | CCATC | 4 cut(s) | 426, 490, 1123, 1664 |
| BceAI | ACGGC | 1 cut(s) | 624 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 454, 488 |
| BciVI | GTATCC | 1 cut(s) | 847 |
| BclI | TGATCA | 1 cut(s) | 586 |
| BcoDI | GTCTC | 2 cut(s) | 519, 1305 |
| BfaI | CTAG | 5 cut(s) | 32, 86, 1649, 1655, 1842 |
| BfmI | CTRYAG | 2 cut(s) | 111, 1794 |
| BfrI | CTTAAG | 2 cut(s) | 404, 1319 |
| BfuAI | ACCTGC | 1 cut(s) | 416 |
| BfuI | GTATCC | 1 cut(s) | 847 |
| BglII | AGATCT | 3 cut(s) | 363, 811, 1844 |
| BmcAI | AGTACT | 1 cut(s) | 1840 |
| Bme18I | GGWCC | 4 cut(s) | 267, 436, 1730, 1874 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1736 |
| BmgT120I | GGNCC | 4 cut(s) | 267, 436, 1730, 1874 |
| BmiI | GGNNCC | 4 cut(s) | 269, 437, 870, 1876 |
| BmsI | GCATC | 6 cut(s) | 606, 630, 712, 1327, 1405, 1696 |
| BpiI | GAAGAC | 3 cut(s) | 1081, 1088, 1259 |
| Bpu14I | TTCGAA | 2 cut(s) | 139, 1472 |
| BsaBI | GATNNNNATC | 1 cut(s) | 620 |
| BsaHI | GRCGYC | 1 cut(s) | 1575 |
| BsaJI | CCNNGG | 1 cut(s) | 171 |
| Bse1I | ACTGG | 2 cut(s) | 1733, 1767 |
| Bse21I | CCTNAGG | 1 cut(s) | 129 |
| Bse3DI | GCAATG | 2 cut(s) | 477, 1239 |
| Bse8I | GATNNNNATC | 1 cut(s) | 620 |
| BseDI | CCNNGG | 1 cut(s) | 171 |
| BseGI | GGATG | 8 cut(s) | 437, 621, 727, 1099, 1420, 1675, 1711, 1780 |
| BseJI | GATNNNNATC | 1 cut(s) | 620 |
| BseMI | GCAATG | 2 cut(s) | 477, 1239 |
| BseMII | CTCAG | 3 cut(s) | 143, 402, 719 |
| BseNI | ACTGG | 2 cut(s) | 1733, 1767 |
| BseRI | GAGGAG | 1 cut(s) | 92 |
| BseSI | GKGCMC | 1 cut(s) | 1188 |
| BseXI | GCAGC | 8 cut(s) | 363, 513, 931, 934, 955, 1216, 1726, 1783 |
| Bsh1236I | CGCG | 1 cut(s) | 1809 |
| BshFI | GGCC | 1 cut(s) | 1637 |
| BslFI | GGGAC | 3 cut(s) | 268, 1445, 1804 |
| BsmAI | GTCTC | 2 cut(s) | 519, 1305 |
| BsmFI | GGGAC | 3 cut(s) | 268, 1445, 1804 |
| BsmI | GAATGC | 3 cut(s) | 684, 1130, 1808 |
| BsnI | GGCC | 1 cut(s) | 1637 |
| Bsp119I | TTCGAA | 2 cut(s) | 139, 1472 |
| Bsp1286I | GDGCHC | 3 cut(s) | 79, 193, 1188 |
| Bsp1407I | TGTACA | 2 cut(s) | 319, 1157 |
| Bsp143I | GATC | 9 cut(s) | 363, 586, 736, 811, 868, 1015, 1603, 1698, 1844 |
| BspACI | CCGC | 6 cut(s) | 106, 682, 872, 895, 903, 1807 |
| BspANI | GGCC | 1 cut(s) | 1637 |
| BspCNI | CTCAG | 3 cut(s) | 142, 403, 720 |
| BspFNI | CGCG | 1 cut(s) | 1809 |
| BspLI | GGNNCC | 4 cut(s) | 269, 437, 870, 1876 |
| BspMAI | CTGCAG | 1 cut(s) | 1798 |
| BspMI | ACCTGC | 1 cut(s) | 416 |
| BspPI | GGATC | 3 cut(s) | 744, 863, 876 |
| BspT104I | TTCGAA | 2 cut(s) | 139, 1472 |
| BspTI | CTTAAG | 2 cut(s) | 404, 1319 |
| BsrDI | GCAATG | 2 cut(s) | 477, 1239 |
| BsrGI | TGTACA | 2 cut(s) | 319, 1157 |
| BsrI | ACTGG | 2 cut(s) | 1733, 1767 |
| BssECI | CCNNGG | 1 cut(s) | 171 |
| BssMI | GATC | 9 cut(s) | 363, 586, 736, 811, 868, 1015, 1603, 1698, 1844 |
| BssNAI | GTATAC | 1 cut(s) | 237 |
| BssNI | GRCGYC | 1 cut(s) | 1575 |
| BssT1I | CCWWGG | 1 cut(s) | 171 |
| Bst1107I | GTATAC | 1 cut(s) | 237 |
| Bst4CI | ACNGT | 6 cut(s) | 112, 319, 671, 1107, 1348, 1560 |
| Bst6I | CTCTTC | 5 cut(s) | 351, 543, 657, 1438, 1837 |
| BstACI | GRCGYC | 1 cut(s) | 1575 |
| BstAFI | CTTAAG | 2 cut(s) | 404, 1319 |
| BstAUI | TGTACA | 2 cut(s) | 319, 1157 |
| BstBI | TTCGAA | 2 cut(s) | 139, 1472 |
| BstC8I | GCNNGC | 2 cut(s) | 159, 1776 |
| BstDEI | CTNAG | 4 cut(s) | 129, 411, 728, 1247 |
| BstF5I | GGATG | 8 cut(s) | 437, 621, 727, 1099, 1420, 1675, 1711, 1780 |
| BstFNI | CGCG | 1 cut(s) | 1809 |
| BstHHI | GCGC | 1 cut(s) | 621 |
| BstKTI | GATC | 9 cut(s) | 366, 589, 739, 814, 871, 1018, 1606, 1701, 1847 |
| BstMAI | GTCTC | 2 cut(s) | 519, 1305 |
| BstMBI | GATC | 9 cut(s) | 363, 586, 736, 811, 868, 1015, 1603, 1698, 1844 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 385, 977, 1424, 1634, 1756 |
| BstNSI | RCATGY | 1 cut(s) | 326 |
| BstSFI | CTRYAG | 2 cut(s) | 111, 1794 |
| BstSLI | GKGCMC | 1 cut(s) | 1188 |
| BstUI | CGCG | 1 cut(s) | 1809 |
| BstV1I | GCAGC | 8 cut(s) | 363, 513, 931, 934, 955, 1216, 1726, 1783 |
| BstV2I | GAAGAC | 3 cut(s) | 1081, 1088, 1259 |
| BstX2I | RGATCY | 5 cut(s) | 363, 736, 811, 868, 1844 |
| BstYI | RGATCY | 5 cut(s) | 363, 736, 811, 868, 1844 |
| BstZ17I | GTATAC | 1 cut(s) | 237 |
| Bsu36I | CCTNAGG | 1 cut(s) | 129 |
| BsuI | GTATCC | 1 cut(s) | 847 |
| BsuRI | GGCC | 1 cut(s) | 1637 |
| BtgZI | GCGATG | 1 cut(s) | 1350 |
| BtsCI | GGATG | 8 cut(s) | 437, 621, 727, 1099, 1420, 1675, 1711, 1780 |
| BtsIMutI | CAGTG | 4 cut(s) | 108, 676, 725, 1112 |
| BveI | ACCTGC | 1 cut(s) | 416 |
| Cac8I | GCNNGC | 2 cut(s) | 159, 1776 |
| CfoI | GCGC | 1 cut(s) | 621 |
| Cfr13I | GGNCC | 4 cut(s) | 267, 436, 1730, 1874 |
| Csp6I | GTAC | 5 cut(s) | 320, 667, 1158, 1403, 1839 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 282, 317 |
| CviAII | CATG | 6 cut(s) | 323, 633, 1161, 1258, 1702, 1767 |
| CviQI | GTAC | 5 cut(s) | 320, 667, 1158, 1403, 1839 |
| DdeI | CTNAG | 4 cut(s) | 129, 411, 728, 1247 |
| DpnI | GATC | 9 cut(s) | 365, 588, 738, 813, 870, 1017, 1605, 1700, 1846 |
| DpnII | GATC | 9 cut(s) | 363, 586, 736, 811, 868, 1015, 1603, 1698, 1844 |
| DraIII | CACNNNGTG | 1 cut(s) | 1109 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1736 |
| Eam1104I | CTCTTC | 5 cut(s) | 351, 543, 657, 1438, 1837 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1736 |
| EarI | CTCTTC | 5 cut(s) | 351, 543, 657, 1438, 1837 |
| Eco130I | CCWWGG | 1 cut(s) | 171 |
| Eco24I | GRGCYC | 2 cut(s) | 79, 193 |
| Eco47I | GGWCC | 4 cut(s) | 267, 436, 1730, 1874 |
| Eco57I | CTGAAG | 4 cut(s) | 375, 1075, 1449, 1838 |
| Eco81I | CCTNAGG | 1 cut(s) | 129 |
| EcoO109I | RGGNCCY | 1 cut(s) | 267 |
| EcoRI | GAATTC | 1 cut(s) | 35 |
| EcoT14I | CCWWGG | 1 cut(s) | 171 |
| EcoT22I | ATGCAT | 1 cut(s) | 1369 |
| EcoT38I | GRGCYC | 2 cut(s) | 79, 193 |
| ErhI | CCWWGG | 1 cut(s) | 171 |
| FaeI | CATG | 6 cut(s) | 326, 636, 1164, 1261, 1705, 1770 |
| FaqI | GGGAC | 3 cut(s) | 268, 1445, 1804 |
| FatI | CATG | 6 cut(s) | 322, 632, 1160, 1257, 1701, 1766 |
| FbaI | TGATCA | 1 cut(s) | 586 |
| FblI | GTMKAC | 2 cut(s) | 114, 236 |
| FokI | GGATG | 8 cut(s) | 444, 628, 734, 1086, 1427, 1682, 1718, 1767 |
| FriOI | GRGCYC | 2 cut(s) | 79, 193 |
| FspAI | RTGCGCAY | 1 cut(s) | 620 |
| FspBI | CTAG | 5 cut(s) | 32, 86, 1649, 1655, 1842 |
| FspI | TGCGCA | 1 cut(s) | 620 |
| GlaI | GCGC | 1 cut(s) | 620 |
| HaeIII | GGCC | 1 cut(s) | 1637 |
| HhaI | GCGC | 1 cut(s) | 621 |
| Hin1I | GRCGYC | 1 cut(s) | 1575 |
| Hin1II | CATG | 6 cut(s) | 326, 636, 1164, 1261, 1705, 1770 |
| Hin6I | GCGC | 1 cut(s) | 619 |
| HinP1I | GCGC | 1 cut(s) | 619 |
| HindIII | AAGCTT | 1 cut(s) | 446 |
| HinfI | GANTC | 9 cut(s) | 256, 561, 835, 915, 1165, 1313, 1329, 1478, 1662 |
| Hpy166II | GTNNAC | 5 cut(s) | 115, 237, 309, 649, 1730 |
| Hpy188I | TCNGA | 8 cut(s) | 72, 228, 286, 914, 1020, 1120, 1310, 1490 |
| Hpy188III | TCNNGA | 7 cut(s) | 51, 86, 260, 584, 1029, 1294, 1655 |
| Hpy8I | GTNNAC | 5 cut(s) | 115, 237, 309, 649, 1730 |
| Hpy99I | CGWCG | 1 cut(s) | 1577 |
| HpyAV | CCTTC | 9 cut(s) | 244, 281, 511, 726, 1204, 1206, 1250, 1794, 1813 |
| HpyCH4III | ACNGT | 6 cut(s) | 112, 319, 671, 1107, 1348, 1560 |
| HpyCH4IV | ACGT | 2 cut(s) | 655, 1575 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 385, 977, 1424, 1634, 1756 |
| HpyF3I | CTNAG | 4 cut(s) | 129, 411, 728, 1247 |
| HpySE526I | ACGT | 2 cut(s) | 655, 1575 |
| Hsp92I | GRCGYC | 1 cut(s) | 1575 |
| Hsp92II | CATG | 6 cut(s) | 326, 636, 1164, 1261, 1705, 1770 |
| HspAI | GCGC | 1 cut(s) | 619 |
| Ksp22I | TGATCA | 1 cut(s) | 586 |
| Kzo9I | GATC | 9 cut(s) | 363, 586, 736, 811, 868, 1015, 1603, 1698, 1844 |
| LmnI | GCTCC | 4 cut(s) | 46, 176, 290, 1004 |
| Lsp1109I | GCAGC | 8 cut(s) | 363, 513, 931, 934, 955, 1216, 1726, 1783 |
| LweI | GCATC | 6 cut(s) | 606, 630, 712, 1327, 1405, 1696 |
| MaeI | CTAG | 5 cut(s) | 32, 86, 1649, 1655, 1842 |
| MaeII | ACGT | 2 cut(s) | 655, 1575 |
| MaeIII | GTNAC | 5 cut(s) | 148, 832, 1064, 1141, 1907 |
| MalI | GATC | 9 cut(s) | 365, 588, 738, 813, 870, 1017, 1605, 1700, 1846 |
| MboI | GATC | 9 cut(s) | 363, 586, 736, 811, 868, 1015, 1603, 1698, 1844 |
| MflI | RGATCY | 5 cut(s) | 363, 736, 811, 868, 1844 |
| MhlI | GDGCHC | 3 cut(s) | 79, 193, 1188 |
| MlyI | GAGTC | 5 cut(s) | 250, 829, 1307, 1338, 1671 |
| Mph1103I | ATGCAT | 1 cut(s) | 1369 |
| MroXI | GAANNNNTTC | 1 cut(s) | 552 |
| MseI | TTAA | 9 cut(s) | 405, 627, 1320, 1353, 1496, 1521, 1580, 1686, 1719 |
| MspCI | CTTAAG | 2 cut(s) | 404, 1319 |
| Mva1269I | GAATGC | 3 cut(s) | 684, 1130, 1808 |
| MvnI | CGCG | 1 cut(s) | 1809 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 385, 977, 1424, 1634, 1756 |
| NdeII | GATC | 9 cut(s) | 363, 586, 736, 811, 868, 1015, 1603, 1698, 1844 |
| NlaIII | CATG | 6 cut(s) | 326, 636, 1164, 1261, 1705, 1770 |
| NlaIV | GGNNCC | 4 cut(s) | 269, 437, 870, 1876 |
| NmuCI | GTSAC | 3 cut(s) | 832, 1064, 1141 |
| NsbI | TGCGCA | 1 cut(s) | 620 |
| NsiI | ATGCAT | 1 cut(s) | 1369 |
| NspI | RCATGY | 1 cut(s) | 326 |
| NspV | TTCGAA | 2 cut(s) | 139, 1472 |
| PaqCI | CACCTGC | 1 cut(s) | 416 |
| PciI | ACATGT | 1 cut(s) | 322 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 883 |
| PctI | GAATGC | 3 cut(s) | 684, 1130, 1808 |
| PdmI | GAANNNNTTC | 1 cut(s) | 552 |
| PfeI | GAWTC | 4 cut(s) | 561, 915, 1165, 1478 |
| PleI | GAGTC | 5 cut(s) | 250, 829, 1307, 1337, 1670 |
| PpsI | GAGTC | 5 cut(s) | 250, 829, 1307, 1337, 1670 |
| PpuMI | RGGWCCY | 1 cut(s) | 267 |
| PscI | ACATGT | 1 cut(s) | 322 |
| Psp5II | RGGWCCY | 1 cut(s) | 267 |
| PspN4I | GGNNCC | 4 cut(s) | 269, 437, 870, 1876 |
| PspPI | GGNCC | 4 cut(s) | 267, 436, 1730, 1874 |
| PspPPI | RGGWCCY | 1 cut(s) | 267 |
| PstI | CTGCAG | 1 cut(s) | 1798 |
| PsuI | RGATCY | 5 cut(s) | 363, 736, 811, 868, 1844 |
| RsaI | GTAC | 5 cut(s) | 321, 668, 1159, 1404, 1840 |
| RsaNI | GTAC | 5 cut(s) | 320, 667, 1158, 1403, 1839 |
| SaqAI | TTAA | 9 cut(s) | 405, 627, 1320, 1353, 1496, 1521, 1580, 1686, 1719 |
| Sau3AI | GATC | 9 cut(s) | 363, 586, 736, 811, 868, 1015, 1603, 1698, 1844 |
| Sau96I | GGNCC | 4 cut(s) | 267, 436, 1730, 1874 |
| ScaI | AGTACT | 1 cut(s) | 1840 |
| SchI | GAGTC | 5 cut(s) | 250, 829, 1307, 1338, 1671 |
| SduI | GDGCHC | 3 cut(s) | 79, 193, 1188 |
| SfaNI | GCATC | 6 cut(s) | 606, 630, 712, 1327, 1405, 1696 |
| SfcI | CTRYAG | 2 cut(s) | 111, 1794 |
| SfuI | TTCGAA | 2 cut(s) | 139, 1472 |
| SinI | GGWCC | 4 cut(s) | 267, 436, 1730, 1874 |
| SmlI | CTYRAG | 2 cut(s) | 404, 1319 |
| SmoI | CTYRAG | 2 cut(s) | 404, 1319 |
| SsiI | CCGC | 6 cut(s) | 106, 682, 872, 895, 903, 1807 |
| SspMI | CTAG | 5 cut(s) | 32, 86, 1649, 1655, 1842 |
| StyI | CCWWGG | 1 cut(s) | 171 |
| TaaI | ACNGT | 6 cut(s) | 112, 319, 671, 1107, 1348, 1560 |
| TaiI | ACGT | 2 cut(s) | 658, 1578 |
| TaqI | TCGA | 7 cut(s) | 52, 64, 139, 474, 886, 924, 1472 |
| TatI | WGTACW | 4 cut(s) | 319, 666, 1157, 1838 |
| TauI | GCSGC | 3 cut(s) | 684, 905, 1809 |
| TfiI | GAWTC | 4 cut(s) | 561, 915, 1165, 1478 |
| Tru1I | TTAA | 9 cut(s) | 405, 627, 1320, 1353, 1496, 1521, 1580, 1686, 1719 |
| Tru9I | TTAA | 9 cut(s) | 405, 627, 1320, 1353, 1496, 1521, 1580, 1686, 1719 |
| TscAI | CASTG | 4 cut(s) | 115, 676, 732, 1112 |
| TseFI | GTSAC | 3 cut(s) | 832, 1064, 1141 |
| TseI | GCWGC | 8 cut(s) | 376, 501, 944, 947, 968, 1229, 1714, 1771 |
| Tsp45I | GTSAC | 3 cut(s) | 832, 1064, 1141 |
| TspDTI | ATGAA | 5 cut(s) | 363, 907, 942, 972, 1088 |
| TspGWI | ACGGA | 2 cut(s) | 428, 769 |
| TspRI | CASTG | 4 cut(s) | 115, 676, 732, 1112 |
| Vha464I | CTTAAG | 2 cut(s) | 404, 1319 |
| VpaK11BI | GGWCC | 4 cut(s) | 267, 436, 1730, 1874 |
| XapI | RAATTY | 2 cut(s) | 35, 1588 |
| XbaI | TCTAGA | 2 cut(s) | 85, 1654 |
| XceI | RCATGY | 1 cut(s) | 326 |
| XmiI | GTMKAC | 2 cut(s) | 114, 236 |
| XmnI | GAANNNNTTC | 1 cut(s) | 552 |
| XspI | CTAG | 5 cut(s) | 32, 86, 1649, 1655, 1842 |
| ZraI | GACGTC | 1 cut(s) | 1576 |
| ZrmI | AGTACT | 1 cut(s) | 1840 |
| Zsp2I | ATGCAT | 1 cut(s) | 1369 |