Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 95 |
| AciI | CCGC | 1 cut(s) | 137 |
| AclWI | GGATC | 4 cut(s) | 141, 154, 314, 357 |
| AcsI | RAATTY | 1 cut(s) | 40 |
| AcuI | CTGAAG | 1 cut(s) | 198 |
| AfaI | GTAC | 2 cut(s) | 5, 249 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 143 |
| AflII | CTTAAG | 1 cut(s) | 62 |
| AgsI | TTSAA | 2 cut(s) | 228, 328 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 485 |
| AluBI | AGCT | 2 cut(s) | 359, 524 |
| AluI | AGCT | 2 cut(s) | 359, 524 |
| AlwI | GGATC | 4 cut(s) | 141, 154, 314, 357 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 89, 414 |
| ApeKI | GCWGC | 2 cut(s) | 301, 521 |
| ApoI | RAATTY | 1 cut(s) | 40 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 27 |
| AspLEI | GCGC | 1 cut(s) | 383 |
| AspS9I | GGNCC | 2 cut(s) | 463, 479 |
| AsuHPI | GGTGA | 1 cut(s) | 49 |
| AvaII | GGWCC | 2 cut(s) | 463, 479 |
| BamHI | GGATCC | 1 cut(s) | 146 |
| BbvI | GCAGC | 2 cut(s) | 288, 533 |
| BfaI | CTAG | 1 cut(s) | 398 |
| BfoI | RGCGCY | 1 cut(s) | 384 |
| BfrI | CTTAAG | 1 cut(s) | 62 |
| BisI | GCNGC | 2 cut(s) | 302, 522 |
| BlsI | GCNGC | 2 cut(s) | 303, 523 |
| BmcAI | AGTACT | 1 cut(s) | 249 |
| Bme18I | GGWCC | 2 cut(s) | 463, 479 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 485 |
| BmgT120I | GGNCC | 2 cut(s) | 463, 479 |
| BmiI | GGNNCC | 2 cut(s) | 148, 193 |
| BmsI | GCATC | 1 cut(s) | 70 |
| BpmI | CTGGAG | 1 cut(s) | 495 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 468, 498 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 143 |
| Bse1I | ACTGG | 2 cut(s) | 150, 482 |
| Bse3DI | GCAATG | 1 cut(s) | 296 |
| BseGI | GGATG | 2 cut(s) | 161, 424 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 143 |
| BseMI | GCAATG | 1 cut(s) | 296 |
| BseMII | CTCAG | 2 cut(s) | 256, 448 |
| BseNI | ACTGG | 2 cut(s) | 150, 482 |
| BseRI | GAGGAG | 1 cut(s) | 161 |
| BseXI | GCAGC | 2 cut(s) | 288, 533 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 143 |
| Bsp1407I | TGTACA | 1 cut(s) | 3 |
| Bsp143I | GATC | 5 cut(s) | 146, 211, 319, 349, 394 |
| BspACI | CCGC | 1 cut(s) | 137 |
| BspCNI | CTCAG | 2 cut(s) | 255, 447 |
| BspLI | GGNNCC | 2 cut(s) | 148, 193 |
| BspPI | GGATC | 4 cut(s) | 141, 154, 314, 357 |
| BspTI | CTTAAG | 1 cut(s) | 62 |
| BsrDI | GCAATG | 1 cut(s) | 296 |
| BsrGI | TGTACA | 1 cut(s) | 3 |
| BsrI | ACTGG | 2 cut(s) | 150, 482 |
| BssMI | GATC | 5 cut(s) | 146, 211, 319, 349, 394 |
| Bst4CI | ACNGT | 3 cut(s) | 91, 252, 292 |
| Bst6I | CTCTTC | 1 cut(s) | 18 |
| BstAFI | CTTAAG | 1 cut(s) | 62 |
| BstAUI | TGTACA | 1 cut(s) | 3 |
| BstC8I | GCNNGC | 2 cut(s) | 164, 526 |
| BstDEI | CTNAG | 2 cut(s) | 242, 434 |
| BstF5I | GGATG | 2 cut(s) | 161, 424 |
| BstH2I | RGCGCY | 1 cut(s) | 384 |
| BstHHI | GCGC | 1 cut(s) | 383 |
| BstKTI | GATC | 5 cut(s) | 149, 214, 322, 352, 397 |
| BstMBI | GATC | 5 cut(s) | 146, 211, 319, 349, 394 |
| BstV1I | GCAGC | 2 cut(s) | 288, 533 |
| BstX2I | RGATCY | 2 cut(s) | 146, 319 |
| BstYI | RGATCY | 2 cut(s) | 146, 319 |
| BtgZI | GCGATG | 1 cut(s) | 93 |
| BtsCI | GGATG | 2 cut(s) | 161, 424 |
| BtsIMutI | CAGTG | 3 cut(s) | 157, 442, 496 |
| Cac8I | GCNNGC | 2 cut(s) | 164, 526 |
| CaiI | CAGNNNCTG | 2 cut(s) | 89, 414 |
| CfoI | GCGC | 1 cut(s) | 383 |
| Cfr13I | GGNCC | 2 cut(s) | 463, 479 |
| Csp6I | GTAC | 2 cut(s) | 4, 248 |
| CviAII | CATG | 1 cut(s) | 517 |
| CviJI | RGCY | 2 cut(s) | 359, 524 |
| CviKI_1 | RGCY | 2 cut(s) | 359, 524 |
| CviQI | GTAC | 2 cut(s) | 4, 248 |
| DdeI | CTNAG | 2 cut(s) | 242, 434 |
| DpnI | GATC | 5 cut(s) | 148, 213, 321, 351, 396 |
| DpnII | GATC | 5 cut(s) | 146, 211, 319, 349, 394 |
| DriI | GACNNNNNGTC | 1 cut(s) | 485 |
| Eam1104I | CTCTTC | 1 cut(s) | 18 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 485 |
| EarI | CTCTTC | 1 cut(s) | 18 |
| Eco47I | GGWCC | 2 cut(s) | 463, 479 |
| Eco57I | CTGAAG | 1 cut(s) | 198 |
| FaeI | CATG | 1 cut(s) | 520 |
| FaiI | YATR | 4 cut(s) | 95, 204, 386, 518 |
| FatI | CATG | 1 cut(s) | 516 |
| Fnu4HI | GCNGC | 2 cut(s) | 302, 522 |
| FokI | GGATG | 2 cut(s) | 168, 431 |
| Fsp4HI | GCNGC | 2 cut(s) | 302, 522 |
| FspBI | CTAG | 1 cut(s) | 398 |
| GlaI | GCGC | 1 cut(s) | 382 |
| GluI | GCNGC | 2 cut(s) | 302, 522 |
| GsuI | CTGGAG | 1 cut(s) | 495 |
| HaeII | RGCGCY | 1 cut(s) | 384 |
| HhaI | GCGC | 1 cut(s) | 383 |
| Hin1II | CATG | 1 cut(s) | 520 |
| Hin6I | GCGC | 1 cut(s) | 381 |
| HinP1I | GCGC | 1 cut(s) | 381 |
| HinfI | GANTC | 4 cut(s) | 28, 133, 233, 411 |
| HphI | GGTGA | 1 cut(s) | 49 |
| Hpy188I | TCNGA | 3 cut(s) | 53, 245, 316 |
| Hpy188III | TCNNGA | 3 cut(s) | 404, 461, 474 |
| HpyCH4III | ACNGT | 3 cut(s) | 91, 252, 292 |
| HpyCH4V | TGCA | 4 cut(s) | 83, 166, 296, 516 |
| HpyF3I | CTNAG | 2 cut(s) | 242, 434 |
| Hsp92II | CATG | 1 cut(s) | 520 |
| HspAI | GCGC | 1 cut(s) | 381 |
| Kzo9I | GATC | 5 cut(s) | 146, 211, 319, 349, 394 |
| LpnPI | CCDG | 8 cut(s) | 45, 163, 208, 400, 446, 459, 495, 523 |
| Lsp1109I | GCAGC | 2 cut(s) | 288, 533 |
| LweI | GCATC | 1 cut(s) | 70 |
| MaeI | CTAG | 1 cut(s) | 398 |
| MalI | GATC | 5 cut(s) | 148, 213, 321, 351, 396 |
| MboI | GATC | 5 cut(s) | 146, 211, 319, 349, 394 |
| MboII | GAAGA | 4 cut(s) | 35, 191, 254, 329 |
| MflI | RGATCY | 2 cut(s) | 146, 319 |
| MluCI | AATT | 3 cut(s) | 40, 273, 387 |
| MlyI | GAGTC | 1 cut(s) | 420 |
| MmeI | TCCRAC | 1 cut(s) | 93 |
| MnlI | CCTC | 3 cut(s) | 182, 340, 470 |
| MroXI | GAANNNNTTC | 1 cut(s) | 27 |
| MseI | TTAA | 4 cut(s) | 63, 276, 453, 468 |
| MspCI | CTTAAG | 1 cut(s) | 62 |
| NdeII | GATC | 5 cut(s) | 146, 211, 319, 349, 394 |
| NlaIII | CATG | 1 cut(s) | 520 |
| NlaIV | GGNNCC | 2 cut(s) | 148, 193 |
| PdmI | GAANNNNTTC | 1 cut(s) | 27 |
| PfeI | GAWTC | 3 cut(s) | 28, 133, 233 |
| PflFI | GACNNNGTC | 1 cut(s) | 410 |
| PkrI | GCNGC | 2 cut(s) | 303, 523 |
| PleI | GAGTC | 1 cut(s) | 419 |
| PpsI | GAGTC | 1 cut(s) | 419 |
| PsiI | TTATAA | 1 cut(s) | 95 |
| PspN4I | GGNNCC | 2 cut(s) | 148, 193 |
| PspPI | GGNCC | 2 cut(s) | 463, 479 |
| PstNI | CAGNNNCTG | 2 cut(s) | 89, 414 |
| PsuI | RGATCY | 2 cut(s) | 146, 319 |
| PsyI | GACNNNGTC | 1 cut(s) | 410 |
| RsaI | GTAC | 2 cut(s) | 5, 249 |
| RsaNI | GTAC | 2 cut(s) | 4, 248 |
| SaqAI | TTAA | 4 cut(s) | 63, 276, 453, 468 |
| SatI | GCNGC | 2 cut(s) | 302, 522 |
| Sau3AI | GATC | 5 cut(s) | 146, 211, 319, 349, 394 |
| Sau96I | GGNCC | 2 cut(s) | 463, 479 |
| ScaI | AGTACT | 1 cut(s) | 249 |
| SchI | GAGTC | 1 cut(s) | 420 |
| SetI | ASST | 4 cut(s) | 361, 403, 468, 526 |
| SfaNI | GCATC | 1 cut(s) | 70 |
| SinI | GGWCC | 2 cut(s) | 463, 479 |
| SmlI | CTYRAG | 1 cut(s) | 62 |
| SmoI | CTYRAG | 1 cut(s) | 62 |
| Sse9I | AATT | 3 cut(s) | 40, 273, 387 |
| SsiI | CCGC | 1 cut(s) | 137 |
| SspMI | CTAG | 1 cut(s) | 398 |
| TaaI | ACNGT | 3 cut(s) | 91, 252, 292 |
| TaqI | TCGA | 1 cut(s) | 352 |
| TasI | AATT | 3 cut(s) | 40, 273, 387 |
| TatI | WGTACW | 2 cut(s) | 3, 247 |
| TfiI | GAWTC | 3 cut(s) | 28, 133, 233 |
| Tru1I | TTAA | 4 cut(s) | 63, 276, 453, 468 |
| Tru9I | TTAA | 4 cut(s) | 63, 276, 453, 468 |
| TscAI | CASTG | 3 cut(s) | 157, 442, 496 |
| TseI | GCWGC | 2 cut(s) | 301, 521 |
| TspDTI | ATGAA | 1 cut(s) | 34 |
| TspGWI | ACGGA | 3 cut(s) | 458, 517, 523 |
| TspRI | CASTG | 3 cut(s) | 157, 442, 496 |
| Tth111I | GACNNNGTC | 1 cut(s) | 410 |
| Vha464I | CTTAAG | 1 cut(s) | 62 |
| VpaK11BI | GGWCC | 2 cut(s) | 463, 479 |
| XapI | RAATTY | 1 cut(s) | 40 |
| XmnI | GAANNNNTTC | 1 cut(s) | 27 |
| XspI | CTAG | 1 cut(s) | 398 |
| ZrmI | AGTACT | 1 cut(s) | 249 |