Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 365 |
| AatII | GACGTC | 1 cut(s) | 1527 |
| Acc16I | TGCGCA | 1 cut(s) | 569 |
| Acc36I | ACCTGC | 1 cut(s) | 365 |
| AccI | GTMKAC | 2 cut(s) | 63, 185 |
| AccII | CGCG | 1 cut(s) | 1758 |
| AccIII | TCCGGA | 1 cut(s) | 208 |
| AciI | CCGC | 6 cut(s) | 55, 631, 821, 852, 1052, 1756 |
| AclWI | GGATC | 3 cut(s) | 693, 812, 825 |
| AcsI | RAATTY | 2 cut(s) | 455, 1537 |
| AcuI | CTGAAG | 4 cut(s) | 324, 1024, 1398, 1787 |
| AcyI | GRCGYC | 1 cut(s) | 1524 |
| AfaI | GTAC | 5 cut(s) | 270, 617, 1108, 1353, 1789 |
| AflII | CTTAAG | 2 cut(s) | 353, 1268 |
| AflIII | ACRYGT | 1 cut(s) | 271 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1685 |
| Alw26I | GTCTC | 2 cut(s) | 468, 1254 |
| AlwI | GGATC | 3 cut(s) | 693, 812, 825 |
| Aor13HI | TCCGGA | 1 cut(s) | 208 |
| AoxI | GGCC | 1 cut(s) | 1584 |
| ApeKI | GCWGC | 8 cut(s) | 325, 450, 823, 893, 896, 917, 1178, 1663 |
| ApoI | RAATTY | 2 cut(s) | 455, 1537 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 501 |
| AspLEI | GCGC | 1 cut(s) | 570 |
| AspS9I | GGNCC | 4 cut(s) | 216, 385, 1679, 1823 |
| AsuHPI | GGTGA | 1 cut(s) | 481 |
| AsuII | TTCGAA | 2 cut(s) | 88, 1421 |
| AvaII | GGWCC | 4 cut(s) | 216, 385, 1679, 1823 |
| AxyI | CCTNAGG | 1 cut(s) | 78 |
| BaeGI | GKGCMC | 1 cut(s) | 1137 |
| BamHI | GGATCC | 1 cut(s) | 817 |
| BanII | GRGCYC | 2 cut(s) | 28, 142 |
| BbsI | GAAGAC | 3 cut(s) | 1030, 1037, 1208 |
| BbvI | GCAGC | 8 cut(s) | 312, 462, 810, 880, 883, 904, 1165, 1675 |
| BccI | CCATC | 2 cut(s) | 375, 439 |
| BceAI | ACGGC | 1 cut(s) | 573 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 403, 437 |
| BciVI | GTATCC | 1 cut(s) | 796 |
| BclI | TGATCA | 1 cut(s) | 535 |
| BcoDI | GTCTC | 2 cut(s) | 468, 1254 |
| BfaI | CTAG | 4 cut(s) | 35, 1598, 1604, 1791 |
| BfmI | CTRYAG | 2 cut(s) | 60, 1743 |
| BfrI | CTTAAG | 2 cut(s) | 353, 1268 |
| BfuAI | ACCTGC | 1 cut(s) | 365 |
| BfuI | GTATCC | 1 cut(s) | 796 |
| BglII | AGATCT | 3 cut(s) | 312, 760, 1793 |
| BmcAI | AGTACT | 1 cut(s) | 1789 |
| Bme18I | GGWCC | 4 cut(s) | 216, 385, 1679, 1823 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1685 |
| BmgT120I | GGNCC | 4 cut(s) | 216, 385, 1679, 1823 |
| BmiI | GGNNCC | 4 cut(s) | 218, 386, 819, 1825 |
| BmsI | GCATC | 7 cut(s) | 555, 579, 661, 1064, 1276, 1354, 1645 |
| BpiI | GAAGAC | 3 cut(s) | 1030, 1037, 1208 |
| Bpu14I | TTCGAA | 2 cut(s) | 88, 1421 |
| BsaBI | GATNNNNATC | 1 cut(s) | 569 |
| BsaHI | GRCGYC | 1 cut(s) | 1524 |
| BsaJI | CCNNGG | 1 cut(s) | 120 |
| BsaWI | WCCGGW | 1 cut(s) | 208 |
| Bse1I | ACTGG | 2 cut(s) | 1682, 1716 |
| Bse21I | CCTNAGG | 1 cut(s) | 78 |
| Bse3DI | GCAATG | 2 cut(s) | 426, 1188 |
| Bse8I | GATNNNNATC | 1 cut(s) | 569 |
| BseAI | TCCGGA | 1 cut(s) | 208 |
| BseDI | CCNNGG | 1 cut(s) | 120 |
| BseGI | GGATG | 8 cut(s) | 386, 570, 676, 1048, 1079, 1369, 1660, 1729 |
| BseJI | GATNNNNATC | 1 cut(s) | 569 |
| BseMI | GCAATG | 2 cut(s) | 426, 1188 |
| BseMII | CTCAG | 3 cut(s) | 92, 351, 668 |
| BseNI | ACTGG | 2 cut(s) | 1682, 1716 |
| BseRI | GAGGAG | 1 cut(s) | 41 |
| BseSI | GKGCMC | 1 cut(s) | 1137 |
| BseXI | GCAGC | 8 cut(s) | 312, 462, 810, 880, 883, 904, 1165, 1675 |
| Bsh1236I | CGCG | 1 cut(s) | 1758 |
| BshFI | GGCC | 1 cut(s) | 1586 |
| BsiSI | CCGG | 1 cut(s) | 209 |
| BslFI | GGGAC | 4 cut(s) | 217, 1394, 1631, 1753 |
| BsmAI | GTCTC | 2 cut(s) | 468, 1254 |
| BsmFI | GGGAC | 4 cut(s) | 217, 1394, 1631, 1753 |
| BsmI | GAATGC | 2 cut(s) | 633, 1757 |
| BsnI | GGCC | 1 cut(s) | 1586 |
| Bsp119I | TTCGAA | 2 cut(s) | 88, 1421 |
| Bsp1286I | GDGCHC | 3 cut(s) | 28, 142, 1137 |
| Bsp13I | TCCGGA | 1 cut(s) | 208 |
| Bsp1407I | TGTACA | 2 cut(s) | 268, 1106 |
| BspACI | CCGC | 6 cut(s) | 55, 631, 821, 852, 1052, 1756 |
| BspANI | GGCC | 1 cut(s) | 1586 |
| BspCNI | CTCAG | 3 cut(s) | 91, 352, 669 |
| BspEI | TCCGGA | 1 cut(s) | 208 |
| BspFNI | CGCG | 1 cut(s) | 1758 |
| BspLI | GGNNCC | 4 cut(s) | 218, 386, 819, 1825 |
| BspMAI | CTGCAG | 1 cut(s) | 1747 |
| BspMI | ACCTGC | 1 cut(s) | 365 |
| BspPI | GGATC | 3 cut(s) | 693, 812, 825 |
| BspT104I | TTCGAA | 2 cut(s) | 88, 1421 |
| BspTI | CTTAAG | 2 cut(s) | 353, 1268 |
| BsrDI | GCAATG | 2 cut(s) | 426, 1188 |
| BsrGI | TGTACA | 2 cut(s) | 268, 1106 |
| BsrI | ACTGG | 2 cut(s) | 1682, 1716 |
| BssECI | CCNNGG | 1 cut(s) | 120 |
| BssNAI | GTATAC | 1 cut(s) | 186 |
| BssNI | GRCGYC | 1 cut(s) | 1524 |
| BssT1I | CCWWGG | 1 cut(s) | 120 |
| Bst1107I | GTATAC | 1 cut(s) | 186 |
| Bst4CI | ACNGT | 4 cut(s) | 61, 268, 620, 1297 |
| Bst6I | CTCTTC | 5 cut(s) | 300, 492, 606, 1387, 1786 |
| BstACI | GRCGYC | 1 cut(s) | 1524 |
| BstAFI | CTTAAG | 2 cut(s) | 353, 1268 |
| BstAUI | TGTACA | 2 cut(s) | 268, 1106 |
| BstBI | TTCGAA | 2 cut(s) | 88, 1421 |
| BstC8I | GCNNGC | 1 cut(s) | 108 |
| BstDEI | CTNAG | 4 cut(s) | 78, 360, 677, 1196 |
| BstF5I | GGATG | 8 cut(s) | 386, 570, 676, 1048, 1079, 1369, 1660, 1729 |
| BstFNI | CGCG | 1 cut(s) | 1758 |
| BstHHI | GCGC | 1 cut(s) | 570 |
| BstMAI | GTCTC | 2 cut(s) | 468, 1254 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 334, 926, 1060, 1373, 1583, 1705 |
| BstNSI | RCATGY | 1 cut(s) | 275 |
| BstSFI | CTRYAG | 2 cut(s) | 60, 1743 |
| BstSLI | GKGCMC | 1 cut(s) | 1137 |
| BstUI | CGCG | 1 cut(s) | 1758 |
| BstV1I | GCAGC | 8 cut(s) | 312, 462, 810, 880, 883, 904, 1165, 1675 |
| BstV2I | GAAGAC | 3 cut(s) | 1030, 1037, 1208 |
| BstX2I | RGATCY | 5 cut(s) | 312, 685, 760, 817, 1793 |
| BstYI | RGATCY | 5 cut(s) | 312, 685, 760, 817, 1793 |
| BstZ17I | GTATAC | 1 cut(s) | 186 |
| Bsu36I | CCTNAGG | 1 cut(s) | 78 |
| BsuI | GTATCC | 1 cut(s) | 796 |
| BsuRI | GGCC | 1 cut(s) | 1586 |
| BtgZI | GCGATG | 1 cut(s) | 1299 |
| BtsCI | GGATG | 8 cut(s) | 386, 570, 676, 1048, 1079, 1369, 1660, 1729 |
| BtsI | GCAGTG | 1 cut(s) | 1061 |
| BtsIMutI | CAGTG | 4 cut(s) | 57, 625, 674, 1061 |
| BveI | ACCTGC | 1 cut(s) | 365 |
| Cac8I | GCNNGC | 1 cut(s) | 108 |
| CfoI | GCGC | 1 cut(s) | 570 |
| Cfr13I | GGNCC | 4 cut(s) | 216, 385, 1679, 1823 |
| Csp6I | GTAC | 5 cut(s) | 269, 616, 1107, 1352, 1788 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 231, 266 |
| CviAII | CATG | 5 cut(s) | 272, 1110, 1207, 1651, 1716 |
| CviQI | GTAC | 5 cut(s) | 269, 616, 1107, 1352, 1788 |
| DdeI | CTNAG | 4 cut(s) | 78, 360, 677, 1196 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1685 |
| Eam1104I | CTCTTC | 5 cut(s) | 300, 492, 606, 1387, 1786 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1685 |
| EarI | CTCTTC | 5 cut(s) | 300, 492, 606, 1387, 1786 |
| Eco130I | CCWWGG | 1 cut(s) | 120 |
| Eco24I | GRGCYC | 2 cut(s) | 28, 142 |
| Eco47I | GGWCC | 4 cut(s) | 216, 385, 1679, 1823 |
| Eco57I | CTGAAG | 4 cut(s) | 324, 1024, 1398, 1787 |
| Eco81I | CCTNAGG | 1 cut(s) | 78 |
| EcoO109I | RGGNCCY | 1 cut(s) | 216 |
| EcoT14I | CCWWGG | 1 cut(s) | 120 |
| EcoT38I | GRGCYC | 2 cut(s) | 28, 142 |
| ErhI | CCWWGG | 1 cut(s) | 120 |
| FaeI | CATG | 5 cut(s) | 275, 1113, 1210, 1654, 1719 |
| FaqI | GGGAC | 4 cut(s) | 217, 1394, 1631, 1753 |
| FatI | CATG | 5 cut(s) | 271, 1109, 1206, 1650, 1715 |
| FbaI | TGATCA | 1 cut(s) | 535 |
| FblI | GTMKAC | 2 cut(s) | 63, 185 |
| FokI | GGATG | 8 cut(s) | 393, 577, 683, 1035, 1086, 1376, 1667, 1716 |
| FriOI | GRGCYC | 2 cut(s) | 28, 142 |
| FspAI | RTGCGCAY | 1 cut(s) | 569 |
| FspBI | CTAG | 4 cut(s) | 35, 1598, 1604, 1791 |
| FspI | TGCGCA | 1 cut(s) | 569 |
| GlaI | GCGC | 1 cut(s) | 569 |
| HaeIII | GGCC | 1 cut(s) | 1586 |
| HapII | CCGG | 1 cut(s) | 209 |
| HhaI | GCGC | 1 cut(s) | 570 |
| Hin1I | GRCGYC | 1 cut(s) | 1524 |
| Hin1II | CATG | 5 cut(s) | 275, 1113, 1210, 1654, 1719 |
| Hin6I | GCGC | 1 cut(s) | 568 |
| HinP1I | GCGC | 1 cut(s) | 568 |
| HindIII | AAGCTT | 1 cut(s) | 395 |
| HinfI | GANTC | 9 cut(s) | 205, 510, 784, 864, 1114, 1262, 1278, 1427, 1611 |
| HpaII | CCGG | 1 cut(s) | 209 |
| HphI | GGTGA | 1 cut(s) | 481 |
| Hpy166II | GTNNAC | 5 cut(s) | 64, 186, 258, 598, 1679 |
| Hpy188I | TCNGA | 7 cut(s) | 177, 235, 863, 969, 1069, 1259, 1439 |
| Hpy188III | TCNNGA | 6 cut(s) | 35, 209, 533, 978, 1243, 1604 |
| Hpy8I | GTNNAC | 5 cut(s) | 64, 186, 258, 598, 1679 |
| Hpy99I | CGWCG | 1 cut(s) | 1526 |
| HpyCH4III | ACNGT | 4 cut(s) | 61, 268, 620, 1297 |
| HpyCH4IV | ACGT | 2 cut(s) | 604, 1524 |
| HpyCH4V | TGCA | 7 cut(s) | 414, 646, 674, 1181, 1367, 1663, 1745 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 334, 926, 1060, 1373, 1583, 1705 |
| HpyF3I | CTNAG | 4 cut(s) | 78, 360, 677, 1196 |
| HpySE526I | ACGT | 2 cut(s) | 604, 1524 |
| Hsp92I | GRCGYC | 1 cut(s) | 1524 |
| Hsp92II | CATG | 5 cut(s) | 275, 1113, 1210, 1654, 1719 |
| HspAI | GCGC | 1 cut(s) | 568 |
| Kpn2I | TCCGGA | 1 cut(s) | 208 |
| Ksp22I | TGATCA | 1 cut(s) | 535 |
| LmnI | GCTCC | 2 cut(s) | 239, 953 |
| Lsp1109I | GCAGC | 8 cut(s) | 312, 462, 810, 880, 883, 904, 1165, 1675 |
| LweI | GCATC | 7 cut(s) | 555, 579, 661, 1064, 1276, 1354, 1645 |
| MaeI | CTAG | 4 cut(s) | 35, 1598, 1604, 1791 |
| MaeII | ACGT | 2 cut(s) | 604, 1524 |
| MaeIII | GTNAC | 5 cut(s) | 97, 781, 1013, 1090, 1856 |
| MflI | RGATCY | 5 cut(s) | 312, 685, 760, 817, 1793 |
| MhlI | GDGCHC | 3 cut(s) | 28, 142, 1137 |
| MlyI | GAGTC | 5 cut(s) | 199, 778, 1256, 1287, 1620 |
| MroI | TCCGGA | 1 cut(s) | 208 |
| MroXI | GAANNNNTTC | 1 cut(s) | 501 |
| MseI | TTAA | 9 cut(s) | 354, 576, 1269, 1302, 1445, 1470, 1529, 1635, 1668 |
| MspA1I | CMGCKG | 1 cut(s) | 823 |
| MspCI | CTTAAG | 2 cut(s) | 353, 1268 |
| MspI | CCGG | 1 cut(s) | 209 |
| Mva1269I | GAATGC | 2 cut(s) | 633, 1757 |
| MvnI | CGCG | 1 cut(s) | 1758 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 334, 926, 1060, 1373, 1583, 1705 |
| NlaIII | CATG | 5 cut(s) | 275, 1113, 1210, 1654, 1719 |
| NlaIV | GGNNCC | 4 cut(s) | 218, 386, 819, 1825 |
| NmuCI | GTSAC | 3 cut(s) | 781, 1013, 1090 |
| NsbI | TGCGCA | 1 cut(s) | 569 |
| NspI | RCATGY | 1 cut(s) | 275 |
| NspV | TTCGAA | 2 cut(s) | 88, 1421 |
| PaqCI | CACCTGC | 1 cut(s) | 365 |
| PciI | ACATGT | 1 cut(s) | 271 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 841 |
| PctI | GAATGC | 2 cut(s) | 633, 1757 |
| PdmI | GAANNNNTTC | 1 cut(s) | 501 |
| PfeI | GAWTC | 4 cut(s) | 510, 864, 1114, 1427 |
| PleI | GAGTC | 5 cut(s) | 199, 778, 1256, 1286, 1619 |
| PpsI | GAGTC | 5 cut(s) | 199, 778, 1256, 1286, 1619 |
| PpuMI | RGGWCCY | 1 cut(s) | 216 |
| PscI | ACATGT | 1 cut(s) | 271 |
| Psp5II | RGGWCCY | 1 cut(s) | 216 |
| PspN4I | GGNNCC | 4 cut(s) | 218, 386, 819, 1825 |
| PspPI | GGNCC | 4 cut(s) | 216, 385, 1679, 1823 |
| PspPPI | RGGWCCY | 1 cut(s) | 216 |
| PstI | CTGCAG | 1 cut(s) | 1747 |
| PsuI | RGATCY | 5 cut(s) | 312, 685, 760, 817, 1793 |
| RsaI | GTAC | 5 cut(s) | 270, 617, 1108, 1353, 1789 |
| RsaNI | GTAC | 5 cut(s) | 269, 616, 1107, 1352, 1788 |
| SaqAI | TTAA | 9 cut(s) | 354, 576, 1269, 1302, 1445, 1470, 1529, 1635, 1668 |
| Sau96I | GGNCC | 4 cut(s) | 216, 385, 1679, 1823 |
| ScaI | AGTACT | 1 cut(s) | 1789 |
| SchI | GAGTC | 5 cut(s) | 199, 778, 1256, 1287, 1620 |
| SduI | GDGCHC | 3 cut(s) | 28, 142, 1137 |
| SfaNI | GCATC | 7 cut(s) | 555, 579, 661, 1064, 1276, 1354, 1645 |
| SfcI | CTRYAG | 2 cut(s) | 60, 1743 |
| SfuI | TTCGAA | 2 cut(s) | 88, 1421 |
| SinI | GGWCC | 4 cut(s) | 216, 385, 1679, 1823 |
| SmlI | CTYRAG | 2 cut(s) | 353, 1268 |
| SmoI | CTYRAG | 2 cut(s) | 353, 1268 |
| SsiI | CCGC | 6 cut(s) | 55, 631, 821, 852, 1052, 1756 |
| SspMI | CTAG | 4 cut(s) | 35, 1598, 1604, 1791 |
| StyI | CCWWGG | 1 cut(s) | 120 |
| TaaI | ACNGT | 4 cut(s) | 61, 268, 620, 1297 |
| TaiI | ACGT | 2 cut(s) | 607, 1527 |
| TaqI | TCGA | 5 cut(s) | 88, 423, 835, 873, 1421 |
| TatI | WGTACW | 4 cut(s) | 268, 615, 1106, 1787 |
| TauI | GCSGC | 3 cut(s) | 633, 854, 1758 |
| TfiI | GAWTC | 4 cut(s) | 510, 864, 1114, 1427 |
| Tru1I | TTAA | 9 cut(s) | 354, 576, 1269, 1302, 1445, 1470, 1529, 1635, 1668 |
| Tru9I | TTAA | 9 cut(s) | 354, 576, 1269, 1302, 1445, 1470, 1529, 1635, 1668 |
| TscAI | CASTG | 4 cut(s) | 64, 625, 681, 1061 |
| TseFI | GTSAC | 3 cut(s) | 781, 1013, 1090 |
| TseI | GCWGC | 8 cut(s) | 325, 450, 823, 893, 896, 917, 1178, 1663 |
| Tsp45I | GTSAC | 3 cut(s) | 781, 1013, 1090 |
| TspDTI | ATGAA | 5 cut(s) | 312, 856, 891, 921, 1037 |
| TspGWI | ACGGA | 4 cut(s) | 377, 718, 833, 1635 |
| TspRI | CASTG | 4 cut(s) | 64, 625, 681, 1061 |
| Vha464I | CTTAAG | 2 cut(s) | 353, 1268 |
| VpaK11BI | GGWCC | 4 cut(s) | 216, 385, 1679, 1823 |
| XapI | RAATTY | 2 cut(s) | 455, 1537 |
| XbaI | TCTAGA | 2 cut(s) | 34, 1603 |
| XceI | RCATGY | 1 cut(s) | 275 |
| XmiI | GTMKAC | 2 cut(s) | 63, 185 |
| XmnI | GAANNNNTTC | 1 cut(s) | 501 |
| XspI | CTAG | 4 cut(s) | 35, 1598, 1604, 1791 |
| ZraI | GACGTC | 1 cut(s) | 1525 |
| ZrmI | AGTACT | 1 cut(s) | 1789 |