pycom17g21760

pre-mRNA-processing-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000798
Physical Location & Seq
Forward (+)
20218046 .. 20219501
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGTGGAACAACGGCCAGATTGCGCCGCCTGGCACCGGCGGTTCGTCGATTCCGCCACCGCCAGCGGCTCAGCCATCTTACACGGTGTTGCCTTCTCCAGCTGATGCCGAGGCTAAGCTTGAAGAGAAGGCCCGGAAATGGCAGCAGCTTAATTCCAAGCGGTATAGCGATAAGCGCAAGTTCGGCTTCGTCGAGTCGCAGAAGGAGGACATGCCTCCTGAACATGTCAGGAAGATAATTAGGGACCATGGAGACATGTCCTCAAAGAAATTTCGTCATGATAAACGAGTGTATCTTGGAGCACTTAAGTTTGTTCCCCATGCCGTTTACAAGCTCCTTGAGAACATGCCAATGCCATGGGAGCAGGTCCGGGATGTGAAGGTTTTGTACCATATAACTGGGGCAATTACATTTATAAATGAAATACCTTGGGTTGTTGAACCCATCTATTTGGCTCAGTGGGGTTCAATGTGGATTATGATGAGAAGGGAGAAGAGGGATCGATCGGCATTTCAAAAGAATGCGGTTTCCACCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000244 GO:0000375 GO:0000377 GO:0000380 GO:0000386 GO:0000387 GO:0000398 GO:0002237 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005682 GO:0005684 GO:0005737 GO:0005911 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009506 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010033 GO:0010467 GO:0016020 GO:0016043 GO:0016070 GO:0016071 GO:0017069 GO:0017070 GO:0022607 GO:0022613 GO:0022618 GO:0030054 GO:0030532 GO:0030619 GO:0030620 GO:0030623 GO:0031593 GO:0032496 GO:0032991 GO:0033993 GO:0034097 GO:0034612 GO:0034622 GO:0034641 GO:0036002 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046540 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0065003 GO:0070530 GO:0070887 GO:0071005 GO:0071007 GO:0071011 GO:0071013 GO:0071216 GO:0071219 GO:0071222 GO:0071310 GO:0071345 GO:0071356 GO:0071396 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097157 GO:0097159 GO:0097525 GO:0097526 GO:0120114 GO:0140030 GO:0140098 GO:1901360 GO:1901363 GO:1901700 GO:1901701 GO:1902494 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
JAB PRO8NT PROCN PROCT PRP8_domainIV RRM_4 U5_2-snRNA_bdg U6-snRNA_bdg

Protein Analysis

180

Amino Acids

20.7

Weight (kDa)

9.75

Isoelectric Point (pI)

54.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRO8NT PF08082 68 - 173 1.3e-59 PRO8NT (NUC069), PrP8 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 416
Acc36I ACCTGC 1 cut(s) 355
AccB1I GGYRCC 1 cut(s) 32
AciI CCGC 7 cut(s) 26, 39, 53, 59, 65, 160, 524
AclWI GGATC 1 cut(s) 507
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 1 cut(s) 269
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 2 cut(s) 35, 138
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 2 cut(s) 223, 255
AgsI TTSAA 4 cut(s) 122, 440, 468, 515
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 4 cut(s) 101, 118, 148, 334
AluI AGCT 4 cut(s) 101, 118, 148, 334
Alw21I GWGCWC 1 cut(s) 304
Alw26I GTCTC 1 cut(s) 246
AlwI GGATC 1 cut(s) 507
AoxI GGCC 2 cut(s) 13, 129
ApeKI GCWGC 2 cut(s) 142, 145
ApoI RAATTY 1 cut(s) 269
AspLEI GCGC 2 cut(s) 25, 177
AspS9I GGNCC 3 cut(s) 130, 244, 367
AsuC2I CCSGG 2 cut(s) 133, 371
AvaII GGWCC 2 cut(s) 244, 367
BaeI ACNNNNGTAYC 2 cut(s) 275, 308
BanI GGYRCC 1 cut(s) 32
BarI GAAGNNNNNNTAC 2 cut(s) 371, 403
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 2 cut(s) 154, 157
BccI CCATC 2 cut(s) 82, 452
BceAI ACGGC 2 cut(s) 28, 308
BciT130I CCWGG 1 cut(s) 30
BcnI CCSGG 2 cut(s) 133, 371
BcoDI GTCTC 1 cut(s) 246
BfrI CTTAAG 1 cut(s) 305
BfuAI ACCTGC 1 cut(s) 355
BisI GCNGC 4 cut(s) 26, 66, 143, 146
BlpI GCTNAGC 2 cut(s) 69, 114
BlsI GCNGC 4 cut(s) 27, 67, 144, 147
Bme1390I CCNGG 3 cut(s) 30, 133, 371
Bme18I GGWCC 2 cut(s) 244, 367
BmgT120I GGNCC 3 cut(s) 130, 244, 367
BmiI GGNNCC 2 cut(s) 34, 245
BmrFI CCNGG 3 cut(s) 30, 133, 371
BmrI ACTGGG 1 cut(s) 408
BmsI GCATC 1 cut(s) 94
BmuI ACTGGG 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 81
Bpu1102I GCTNAGC 2 cut(s) 69, 114
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 2 cut(s) 133, 371
Bsa29I ATCGAT 1 cut(s) 502
BsaJI CCNNGG 4 cut(s) 108, 247, 356, 428
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 138
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 403
BseBI CCWGG 1 cut(s) 30
BseCI ATCGAT 1 cut(s) 502
BseDI CCNNGG 4 cut(s) 108, 247, 356, 428
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 2 cut(s) 35, 138
BseMII CTCAG 2 cut(s) 83, 470
BseNI ACTGG 1 cut(s) 403
BseXI GCAGC 2 cut(s) 154, 157
Bsh1285I CGRYCG 1 cut(s) 506
BshFI GGCC 2 cut(s) 15, 131
BshNI GGYRCC 1 cut(s) 32
BshVI ATCGAT 1 cut(s) 502
BsiEI CGRYCG 1 cut(s) 506
BsiHKAI GWGCWC 1 cut(s) 304
BsiSI CCGG 3 cut(s) 36, 133, 370
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 35, 138
BsmAI GTCTC 1 cut(s) 246
BsmFI GGGAC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 2 cut(s) 15, 131
Bsp1286I GDGCHC 1 cut(s) 304
Bsp143I GATC 2 cut(s) 499, 503
Bsp1720I GCTNAGC 2 cut(s) 69, 114
Bsp19I CCATGG 2 cut(s) 247, 356
BspACI CCGC 7 cut(s) 26, 39, 53, 59, 65, 160, 524
BspANI GGCC 2 cut(s) 15, 131
BspCNI CTCAG 2 cut(s) 82, 469
BspDI ATCGAT 1 cut(s) 502
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 2 cut(s) 34, 245
BspMI ACCTGC 1 cut(s) 355
BspPI GGATC 1 cut(s) 507
BspT107I GGYRCC 1 cut(s) 32
BspTI CTTAAG 1 cut(s) 305
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 1 cut(s) 403
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 4 cut(s) 108, 247, 356, 428
BssMI GATC 2 cut(s) 499, 503
BssT1I CCWWGG 3 cut(s) 247, 356, 428
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 85
Bst6I CTCTTC 2 cut(s) 117, 488
BstAFI CTTAAG 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 3 cut(s) 69, 114, 456
BstDSI CCRYGG 2 cut(s) 247, 356
BstF5I GGATG 1 cut(s) 379
BstHHI GCGC 2 cut(s) 25, 177
BstKTI GATC 2 cut(s) 502, 506
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 2 cut(s) 499, 503
BstMCI CGRYCG 1 cut(s) 506
BstMWI GCNNNNNNNGC 3 cut(s) 174, 183, 361
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 4 cut(s) 214, 227, 259, 349
BstSCI CCNGG 3 cut(s) 28, 131, 369
BstV1I GCAGC 2 cut(s) 154, 157
BstXI CCANNNNNNTGG 3 cut(s) 357, 398, 451
Bsu15I ATCGAT 1 cut(s) 502
BsuRI GGCC 2 cut(s) 15, 131
BsuTUI ATCGAT 1 cut(s) 502
BtgI CCRYGG 2 cut(s) 247, 356
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 1 cut(s) 464
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 1 cut(s) 63
CciI TCATGA 1 cut(s) 277
CfoI GCGC 2 cut(s) 25, 177
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 3 cut(s) 130, 244, 367
ClaI ATCGAT 1 cut(s) 502
Csp6I GTAC 1 cut(s) 388
CviAII CATG 8 cut(s) 211, 224, 248, 256, 278, 320, 346, 357
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 3 cut(s) 69, 114, 456
DpnI GATC 2 cut(s) 501, 505
DpnII GATC 2 cut(s) 499, 503
EaeI YGGCCR 1 cut(s) 13
Eam1104I CTCTTC 2 cut(s) 117, 488
EarI CTCTTC 2 cut(s) 117, 488
EciI GGCGGA 1 cut(s) 42
Eco130I CCWWGG 3 cut(s) 247, 356, 428
Eco47I GGWCC 2 cut(s) 244, 367
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 3 cut(s) 247, 356, 428
ErhI CCWWGG 3 cut(s) 247, 356, 428
FaeI CATG 8 cut(s) 214, 227, 251, 259, 281, 323, 349, 360
FalI AAGNNNNNCTT 2 cut(s) 170, 202
FaqI GGGAC 1 cut(s) 257
FatI CATG 8 cut(s) 210, 223, 247, 255, 277, 319, 345, 356
Fnu4HI GCNGC 4 cut(s) 26, 66, 143, 146
FokI GGATG 1 cut(s) 386
Fsp4HI GCNGC 4 cut(s) 26, 66, 143, 146
GlaI GCGC 2 cut(s) 24, 176
GluI GCNGC 4 cut(s) 26, 66, 143, 146
GsuI CTGGAG 1 cut(s) 81
HaeIII GGCC 2 cut(s) 15, 131
HapII CCGG 3 cut(s) 36, 133, 370
HhaI GCGC 2 cut(s) 25, 177
Hin1II CATG 8 cut(s) 214, 227, 251, 259, 281, 323, 349, 360
Hin6I GCGC 2 cut(s) 23, 175
HinP1I GCGC 2 cut(s) 23, 175
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 2 cut(s) 49, 194
HpaII CCGG 3 cut(s) 36, 133, 370
Hpy166II GTNNAC 1 cut(s) 328
Hpy188III TCNNGA 3 cut(s) 218, 229, 278
Hpy8I GTNNAC 1 cut(s) 328
Hpy99I CGWCG 2 cut(s) 49, 194
HpyAV CCTTC 5 cut(s) 102, 121, 196, 373, 480
HpyCH4III ACNGT 1 cut(s) 85
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 183, 361
HpyF3I CTNAG 3 cut(s) 69, 114, 456
Hsp92II CATG 8 cut(s) 214, 227, 251, 259, 281, 323, 349, 360
HspAI GCGC 2 cut(s) 23, 175
Kzo9I GATC 2 cut(s) 499, 503
LmnI GCTCC 3 cut(s) 299, 339, 361
Lsp1109I GCAGC 2 cut(s) 154, 157
LweI GCATC 1 cut(s) 94
MalI GATC 2 cut(s) 501, 505
MboI GATC 2 cut(s) 499, 503
MboII GAAGA 3 cut(s) 134, 244, 505
MhlI GDGCHC 1 cut(s) 304
MluCI AATT 4 cut(s) 151, 237, 269, 405
MlyI GAGTC 1 cut(s) 203
MnlI CCTC 5 cut(s) 103, 199, 225, 271, 489
MseI TTAA 2 cut(s) 150, 306
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 2 cut(s) 65, 101
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 3 cut(s) 36, 133, 370
MspR9I CCNGG 3 cut(s) 30, 133, 371
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 3 cut(s) 174, 183, 361
NciI CCSGG 2 cut(s) 133, 371
NcoI CCATGG 2 cut(s) 247, 356
NdeII GATC 2 cut(s) 499, 503
NlaIII CATG 8 cut(s) 214, 227, 251, 259, 281, 323, 349, 360
NlaIV GGNNCC 2 cut(s) 34, 245
NmeAIII GCCGAG 1 cut(s) 133
NspI RCATGY 4 cut(s) 214, 227, 259, 349
PagI TCATGA 1 cut(s) 277
PciI ACATGT 2 cut(s) 223, 255
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 1 cut(s) 49
PfoI TCCNGGA 1 cut(s) 369
PkrI GCNGC 4 cut(s) 27, 67, 144, 147
Ple19I CGATCG 1 cut(s) 506
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
PscI ACATGT 2 cut(s) 223, 255
PsiI TTATAA 1 cut(s) 416
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 2 cut(s) 34, 245
PspPI GGNCC 3 cut(s) 130, 244, 367
PvuI CGATCG 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 1 cut(s) 350
SaqAI TTAA 2 cut(s) 150, 306
SatI GCNGC 4 cut(s) 26, 66, 143, 146
Sau3AI GATC 2 cut(s) 499, 503
Sau96I GGNCC 3 cut(s) 130, 244, 367
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 3 cut(s) 30, 133, 371
SduI GDGCHC 1 cut(s) 304
SetI ASST 7 cut(s) 103, 120, 150, 336, 369, 384, 430
SfaNI GCATC 1 cut(s) 94
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 2 cut(s) 244, 367
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 305, 338
SmoI CTYRAG 2 cut(s) 305, 338
Sse9I AATT 4 cut(s) 151, 237, 269, 405
SsiI CCGC 7 cut(s) 26, 39, 53, 59, 65, 160, 524
StyD4I CCNGG 3 cut(s) 28, 131, 369
StyI CCWWGG 3 cut(s) 247, 356, 428
TaaI ACNGT 1 cut(s) 85
TaqI TCGA 3 cut(s) 47, 192, 502
TasI AATT 4 cut(s) 151, 237, 269, 405
TauI GCSGC 2 cut(s) 28, 68
TfiI GAWTC 1 cut(s) 49
Tru1I TTAA 2 cut(s) 150, 306
Tru9I TTAA 2 cut(s) 150, 306
TscAI CASTG 1 cut(s) 464
TseI GCWGC 2 cut(s) 142, 145
TspDTI ATGAA 1 cut(s) 435
TspRI CASTG 1 cut(s) 464
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 2 cut(s) 244, 367
XapI RAATTY 1 cut(s) 269
XceI RCATGY 4 cut(s) 214, 227, 259, 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.