Rmu_sc0004165.1_g000032

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004165.1
OG0000890
Physical Location & Seq
Reverse (-)
102357 .. 103543
1187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004165.1_g000032.1.cds

Sequence Viewer

Length: 489 bp
atggattcacttgttgcagtcaagttggttctctctcctattgatcatctccatcctctttatagtattgtggcaagttgtcaagagttttttagtagggattggaatgtgtcccttacccatgtttacagagaatgtaatttagtggctgatttgttagcaaatattggactcgagctcctcctaaactcgagctcctcttcctcctcctcaccatcacaatcctccctcctccattctcaggcggaatctgaagcgccgagatcccgacctcgatctaaaattgaagtaacagctctgaatcttgagcccggcctcaatctaagattcgcagagttgaagaaattaaagaggaatggttctttcctctcaagccccaccaacgtctgggttagagcccaaaacccgaacacagcaacccagctgcactcaactgcgccgtttctgactctctccgtgactctgtgtctccgtctccgccgtttctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MATH RVT_3 zf-RVT

Protein Analysis

162

Amino Acids

17.97

Weight (kDa)

9.25

Isoelectric Point (pI)

64.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42905
fragaria_vesca FvH4_1g15070 FvH4_1g21661 FvH4_1g24182 FvH4_2g15861 FvH4_7g26563
prunus_persica Prupe.1G097800_v2.0.a1 Prupe.1G446500_v2.0.a1 Prupe.4G161900_v2.0.a1 Prupe.5G085900_v2.0.a1 Prupe.6G118100_v2.0.a1 Prupe.6G201000_v2.0.a1 Prupe.7G199700_v2.0.a1
pyrus_communis pycom07g22740 pycom15g37390 pycom420g00480 pycom420g00490
rosa_chinensis RchiOBHm_Chr1g0319821 RchiOBHm_Chr1g0331331 RchiOBHm_Chr2g0144631 RchiOBHm_Chr2g0146161 RchiOBHm_Chr2g0152401 RchiOBHm_Chr3g0484381 RchiOBHm_Chr3g0484471 RchiOBHm_Chr3g0494391 RchiOBHm_Chr4g0411801 RchiOBHm_Chr4g0426191 RchiOBHm_Chr5g0016411 RchiOBHm_Chr5g0074971 RchiOBHm_Chr6g0262791 RchiOBHm_Chr6g0272021 RchiOBHm_Chr7g0181271 RchiOBHm_Chr7g0224511 RchiOBHm_Chr7g0225251
rosa_laevigata RLG00000017287
rosa_multiflora Rmu_co8215414.1_g000001 Rmu_sc0001850.1_g000028 Rmu_sc0002132.1_g000045 Rmu_sc0003623.1_g000032 Rmu_sc0003826.1_g000010 Rmu_sc0004165.1_g000032 Rmu_sc0004805.1_g000052 Rmu_sc0005837.1_g000007 Rmu_sc0011261.1_g000003 Rmu_sc0012066.1_g000001 Rmu_sc0021240.1_g000002 Rmu_sc0028377.1_g000003 Rmu_ssc0000454.1_g000003
rosa_roxburghii Rroxscaffold_177G00434230 Rroxscaffold_180G00433210 Rroxscaffold_1G00050960 Rroxscaffold_2G00112540 Rroxscaffold_7G00172420
rosa_rugosa Rorug02G0446000 Rorug03G0370600 Rorug04G0217600.1 Rorug07G0160200 Rorug07G0292300.1
rosa_samantha Rh3AG161100 Rh5AG403600 Rh5CG315600 Rh5CG315800 Rh6BG408500 Rh7CG313500
rosa_wichuraiana Rw4G023910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 387
AciI CCGC 2 cut(s) 245, 478
AclWI GGATC 1 cut(s) 258
AcuI CTGAAG 1 cut(s) 273
AfiI CCNNNNNNNGG 2 cut(s) 241, 387
AgsI TTSAA 2 cut(s) 287, 340
AhdI GACNNNNNGTC 1 cut(s) 465
AluBI AGCT 4 cut(s) 178, 195, 296, 424
AluI AGCT 4 cut(s) 178, 195, 296, 424
Alw21I GWGCWC 2 cut(s) 180, 197
Alw26I GTCTC 2 cut(s) 473, 479
AlwI GGATC 1 cut(s) 258
Ama87I CYCGRG 2 cut(s) 173, 190
AoxI GGCC 1 cut(s) 313
ApeKI GCWGC 1 cut(s) 424
AspLEI GCGC 2 cut(s) 259, 439
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 204
AvaI CYCGRG 2 cut(s) 173, 190
BanII GRGCYC 4 cut(s) 180, 197, 312, 400
Bbv12I GWGCWC 2 cut(s) 180, 197
BbvI GCAGC 1 cut(s) 411
BccI CCATC 2 cut(s) 60, 223
BceAI ACGGC 2 cut(s) 424, 465
BclI TGATCA 1 cut(s) 43
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 2 cut(s) 473, 479
BfoI RGCGCY 1 cut(s) 260
BisI GCNGC 1 cut(s) 425
BlsI GCNGC 1 cut(s) 426
Bme1390I CCNGG 1 cut(s) 312
BmeRI GACNNNNNGTC 1 cut(s) 465
BmeT110I CYCGRG 2 cut(s) 173, 190
BmrFI CCNGG 1 cut(s) 312
BpuEI CTTGAG 2 cut(s) 326, 355
BpuMI CCSGG 1 cut(s) 312
BsaXI ACNNNNNCTCC 4 cut(s) 162, 179, 192, 209
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 387
BseGI GGATG 1 cut(s) 52
BseLI CCNNNNNNNGG 2 cut(s) 241, 387
BseMII CTCAG 1 cut(s) 254
BseRI GAGGAG 5 cut(s) 170, 187, 196, 199, 221
BseXI GCAGC 1 cut(s) 411
BseYI CCCAGC 1 cut(s) 420
BsgI GTGCAG 1 cut(s) 410
BshFI GGCC 1 cut(s) 315
BsiHKAI GWGCWC 2 cut(s) 180, 197
BsiHKCI CYCGRG 2 cut(s) 173, 190
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 2 cut(s) 241, 387
BsmAI GTCTC 2 cut(s) 473, 479
BsmBI CGTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 1 cut(s) 315
BsoBI CYCGRG 2 cut(s) 173, 190
Bsp1286I GDGCHC 4 cut(s) 180, 197, 312, 400
Bsp143I GATC 3 cut(s) 43, 263, 275
BspACI CCGC 2 cut(s) 245, 478
BspANI GGCC 1 cut(s) 315
BspCNI CTCAG 1 cut(s) 253
BspPI GGATC 1 cut(s) 258
BssMI GATC 3 cut(s) 43, 263, 275
Bst6I CTCTTC 1 cut(s) 205
BstDEI CTNAG 2 cut(s) 240, 323
BstF5I GGATG 1 cut(s) 52
BstH2I RGCGCY 1 cut(s) 260
BstHHI GCGC 2 cut(s) 259, 439
BstKTI GATC 3 cut(s) 46, 266, 278
BstMAI GTCTC 2 cut(s) 473, 479
BstMBI GATC 3 cut(s) 43, 263, 275
BstSCI CCNGG 1 cut(s) 310
BstV1I GCAGC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 1 cut(s) 315
BtsCI GGATG 1 cut(s) 52
CfoI GCGC 2 cut(s) 259, 439
CviAII CATG 1 cut(s) 122
CviJI RGCY 9 cut(s) 149, 178, 195, 296, 310, 315, 375, 398, 424
CviKI_1 RGCY 9 cut(s) 149, 178, 195, 296, 310, 315, 375, 398, 424
DdeI CTNAG 2 cut(s) 240, 323
DpnI GATC 3 cut(s) 45, 265, 277
DpnII GATC 3 cut(s) 43, 263, 275
DriI GACNNNNNGTC 1 cut(s) 465
Eam1104I CTCTTC 1 cut(s) 205
Eam1105I GACNNNNNGTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 205
EciI GGCGGA 2 cut(s) 260, 467
Ecl136II GAGCTC 2 cut(s) 178, 195
Eco24I GRGCYC 4 cut(s) 180, 197, 312, 400
Eco53kI GAGCTC 2 cut(s) 178, 195
Eco57I CTGAAG 1 cut(s) 273
Eco88I CYCGRG 2 cut(s) 173, 190
EcoICRI GAGCTC 2 cut(s) 178, 195
EcoT38I GRGCYC 4 cut(s) 180, 197, 312, 400
Esp3I CGTCTC 1 cut(s) 479
FaeI CATG 1 cut(s) 125
FaiI YATR 2 cut(s) 63, 123
FaqI GGGAC 1 cut(s) 97
FatI CATG 1 cut(s) 121
FbaI TGATCA 1 cut(s) 43
Fnu4HI GCNGC 1 cut(s) 425
FokI GGATG 1 cut(s) 39
FriOI GRGCYC 4 cut(s) 180, 197, 312, 400
Fsp4HI GCNGC 1 cut(s) 425
GlaI GCGC 2 cut(s) 258, 438
GluI GCNGC 1 cut(s) 425
GsaI CCCAGC 1 cut(s) 424
HaeII RGCGCY 1 cut(s) 260
HaeIII GGCC 1 cut(s) 315
HapII CCGG 1 cut(s) 312
HhaI GCGC 2 cut(s) 259, 439
Hin1II CATG 1 cut(s) 125
Hin6I GCGC 2 cut(s) 257, 437
HinP1I GCGC 2 cut(s) 257, 437
HinfI GANTC 7 cut(s) 5, 171, 248, 301, 327, 448, 460
HpaII CCGG 1 cut(s) 312
HphI GGTGA 1 cut(s) 204
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 4 cut(s) 253, 300, 447, 488
Hpy188III TCNNGA 3 cut(s) 83, 267, 305
Hpy8I GTNNAC 1 cut(s) 127
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 2 cut(s) 17, 427
HpyF3I CTNAG 2 cut(s) 240, 323
HpySE526I ACGT 1 cut(s) 384
Hsp92II CATG 1 cut(s) 125
HspAI GCGC 2 cut(s) 257, 437
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 3 cut(s) 43, 263, 275
LmnI GCTCC 2 cut(s) 183, 200
LpnPI CCDG 4 cut(s) 227, 325, 373, 434
Lsp1109I GCAGC 1 cut(s) 411
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 2 cut(s) 289, 457
MalI GATC 3 cut(s) 45, 265, 277
MboI GATC 3 cut(s) 43, 263, 275
MboII GAAGA 2 cut(s) 192, 352
MflI RGATCY 1 cut(s) 263
MhlI GDGCHC 4 cut(s) 180, 197, 312, 400
MluCI AATT 3 cut(s) 139, 282, 344
MlyI GAGTC 3 cut(s) 165, 442, 454
MseI TTAA 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 424
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 312
NciI CCSGG 1 cut(s) 312
NdeII GATC 3 cut(s) 43, 263, 275
NlaIII CATG 1 cut(s) 125
NmeAIII GCCGAG 1 cut(s) 285
NmuCI GTSAC 1 cut(s) 457
PaeR7I CTCGAG 2 cut(s) 173, 190
PfeI GAWTC 4 cut(s) 5, 248, 301, 327
PflMI CCANNNNNTGG 1 cut(s) 387
PkrI GCNGC 1 cut(s) 426
PleI GAGTC 3 cut(s) 165, 442, 454
PpsI GAGTC 3 cut(s) 165, 442, 454
Psp124BI GAGCTC 2 cut(s) 180, 197
PspFI CCCAGC 1 cut(s) 420
PspXI VCTCGAGB 2 cut(s) 173, 190
PsuI RGATCY 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 424
SacI GAGCTC 2 cut(s) 180, 197
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 1 cut(s) 425
Sau3AI GATC 3 cut(s) 43, 263, 275
SchI GAGTC 3 cut(s) 165, 442, 454
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 4 cut(s) 180, 197, 312, 400
SetI ASST 6 cut(s) 180, 197, 274, 298, 387, 426
Sfr274I CTCGAG 2 cut(s) 173, 190
SlaI CTCGAG 2 cut(s) 173, 190
SmlI CTYRAG 4 cut(s) 173, 190, 305, 370
SmoI CTYRAG 4 cut(s) 173, 190, 305, 370
Sse9I AATT 3 cut(s) 139, 282, 344
SsiI CCGC 2 cut(s) 245, 478
SspI AATATT 1 cut(s) 166
SstI GAGCTC 2 cut(s) 180, 197
StyD4I CCNGG 1 cut(s) 310
TaiI ACGT 1 cut(s) 387
TaqI TCGA 3 cut(s) 174, 191, 274
TasI AATT 3 cut(s) 139, 282, 344
TfiI GAWTC 4 cut(s) 5, 248, 301, 327
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 1 cut(s) 424
Tsp45I GTSAC 1 cut(s) 457
TspGWI ACGGA 2 cut(s) 445, 461
Van91I CCANNNNNTGG 1 cut(s) 387
XhoI CTCGAG 2 cut(s) 173, 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.