Rorug01G0030900

tetratricopeptide repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000877
Physical Location & Seq
Reverse (-)
5238458 .. 5241181
2724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0030900.1

Sequence Viewer

Length: 1680 bp
ATGATATTGAAAAGTGAAATATGGAAGTTGCCAGGTGAAGATATTACTATGCAAGCTCTTTGGTTGAGCTACCATTATCTACCTTCACATCTCAAGCGTTGCTTTGCCTATTGTTCCATATTTCCCAAAGGTTACCAGTTTACAAAATCAGAATTGGTATCGTTATGGATGGCTGAAGATCTCTTACCACTTAGTAAGAGAAAGACGATGGAACAAGTTGGAGATGACTACTTCCTCCTTCTAATCTCAGGGTCTTTTTTTCAACATTCATGTGCTTATACAAAATCAGTGTTCACCATGCATGATCTCATTAGTGATTTGGCAAAGTTTATATCAGGAGAGTTTTGTGTTACGCTTGAGGAGGGTGATGTCTCACTCATCATTATCAGGCCGAGGCCAGGCCAGCTCAAGGCTGGGTTGTACACAGTCGGGAATGACCGGCGCATAAAGGTTGGAAACCAAGAGATAAGAGGCCCTGTGTGGCGGACGTGCGACCTCTGGACCTGGATCAAAGAGGCCAAATTCCGATTGATAGCGACGAGGGCCTTTTCTCTCCAACCCGTGGAAGTGGCAGAGATAGTCGTCGTGCACCATGAAGCGAACCAGGCTCTGGAGCATGCAGAGCAAGCCGGTGGGGTGTCTGAGATGATGACGGTGGCTGCATCTGATGCCCGAATTGGCAAAGGAGTGGTAGCGGCGGCGTCTAGCGTGCTTGGCAGAGGAGCGGAAAGTGGGGTGCTCAGATCATTTTGGAGGCTTGAATGGATTGGGTGGCCCAAATTAGGTTGGGTGCGCAGATCATTTTGGAAGCCCAAAAGGTTGTGGTGGCCCAAATCAGTTTTGAGACTAAAATCAAATGGACCTGGAGTGTCCAAACCTGCTTTGAGTTCCTATTGGAATTGGGCTCTTTGGTGGACCGTGCGAATAGGCTCTGTTTCTTCAGGGTTTCGTATTTGGGATCCCGGAGGATCATTACCCTGCAGCTGGAGGCTCGAGAAAATGGCAAAGGAAAGCTTTAGTGGGGATAGCATTGCAGAGTCAAGTGAGCTTCAGCATTTGCACGGAAGTCTTTTGATCTCAGGACTTGATCACAATGTAGATGATGTGGCTGCTTTGAAGGCCAATTTGAGAGGCAAGGAATATCTCAATGAACTAGGTTTGAAATGGGGAGATGTTACTGAGGGCCCACAAAAAGATGGAGAAGTTCTCAACCAGCTTGAACCTCATCCTATGCTAAGATCACTTGTGATTATATCTTATGGAGGTGAAAGATTTCCAGGTTGGTTAGGAGGTCCTTATTTCTCTAAACTATCTTCTATTGAACTTGTAGATTGTAAGAATTGTGTCCGCTTGCCCCCACTGGAGCAGCTACCCTCCCTGGAGAAGCTTTCTATTAAGGGGTTTGAGGGACTGTGTTTGATTGGCTTCGAGTTTCGTGGCCCATTTAGATCTCCCAAAGAATTGAGTTTCATCAATATGGTCAAATGGCAGAAGTGGTGTTATGTTGACGGTAATCATGAAGAAGGCGGAGTCTTTCCTAATCTTTGTAAGCTGACTCTGGACAACTGTCCGGAGCTAACAAACATTGTACCATTAGACAACTTCCCAAAGCTTGAAGATTTGAGATTGTTGGGTCTGAAGTCCTTTGCTGGTTCACTTTCACAAAGATCTCAATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_2 TPR_8

Protein Analysis

559

Amino Acids

63.03

Weight (kDa)

8.44

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 40 - 108 5.9e-22 Disease resistance protein Winged helix domain
LRR_R13L1-DRL21 PF25019 348 - 467 3e-33 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 794
Acc36I ACCTGC 1 cut(s) 886
AccB7I CCANNNNNTGG 2 cut(s) 562, 610
AccBSI CCGCTC 1 cut(s) 725
AccIII TCCGGA 1 cut(s) 1570
AciI CCGC 6 cut(s) 484, 695, 698, 725, 1348, 1527
AclWI GGATC 4 cut(s) 515, 953, 966, 976
AcsI RAATTY 1 cut(s) 521
AcuI CTGAAG 4 cut(s) 195, 924, 1034, 1658
AcyI GRCGYC 1 cut(s) 701
AfaI GTAC 2 cut(s) 422, 1590
AfiI CCNNNNNNNGG 6 cut(s) 398, 409, 562, 610, 782, 984
AgsI TTSAA 8 cut(s) 10, 263, 761, 1117, 1162, 1220, 1322, 1616
AjiI CACGTC 1 cut(s) 489
AjnI CCWGG 7 cut(s) 31, 397, 503, 603, 862, 1276, 1377
Alw21I GWGCWC 2 cut(s) 591, 741
Alw26I GTCTC 2 cut(s) 376, 838
Alw44I GTGCAC 1 cut(s) 587
AlwI GGATC 4 cut(s) 515, 953, 966, 976
AlwNI CAGNNNCTG 1 cut(s) 610
Ama87I CYCGRG 1 cut(s) 992
Aor13HI TCCGGA 1 cut(s) 1570
ApaI GGGCCC 1 cut(s) 1187
ApaLI GTGCAC 1 cut(s) 587
ApeKI GCWGC 4 cut(s) 659, 981, 1109, 1366
ApoI RAATTY 1 cut(s) 521
Asp700I GAANNNNTTC 1 cut(s) 1272
AspLEI GCGC 2 cut(s) 444, 795
AsuC2I CCSGG 1 cut(s) 963
AsuHPI GGTGA 4 cut(s) 47, 286, 377, 1277
AvaI CYCGRG 1 cut(s) 992
AvaII GGWCC 4 cut(s) 501, 860, 915, 1292
BaeGI GKGCMC 2 cut(s) 591, 1187
BamHI GGATCC 1 cut(s) 958
BanII GRGCYC 2 cut(s) 907, 1187
BarI GAAGNNNNNNTAC 2 cut(s) 168, 200
Bbv12I GWGCWC 2 cut(s) 591, 741
BbvI GCAGC 4 cut(s) 646, 993, 1096, 1378
BccI CCATC 3 cut(s) 163, 202, 1190
BciT130I CCWGG 7 cut(s) 33, 399, 505, 605, 864, 1278, 1379
BclI TGATCA 1 cut(s) 1087
BcnI CCSGG 1 cut(s) 963
BcoDI GTCTC 2 cut(s) 376, 838
BfaI CTAG 2 cut(s) 705, 1154
BfmI CTRYAG 1 cut(s) 979
BfuAI ACCTGC 1 cut(s) 886
BglII AGATCT 3 cut(s) 178, 1448, 1667
BisI GCNGC 6 cut(s) 660, 696, 699, 982, 1110, 1367
BlsI GCNGC 6 cut(s) 661, 697, 700, 983, 1111, 1368
Bme1390I CCNGG 8 cut(s) 33, 399, 505, 605, 864, 963, 1278, 1379
Bme18I GGWCC 4 cut(s) 501, 860, 915, 1292
BmeT110I CYCGRG 1 cut(s) 992
BmgBI CACGTC 1 cut(s) 489
BmiI GGNNCC 2 cut(s) 960, 1185
BmrFI CCNGG 8 cut(s) 33, 399, 505, 605, 864, 963, 1278, 1379
BmsI GCATC 2 cut(s) 658, 671
BoxI GACNNNNGTC 1 cut(s) 1566
BplI GAGNNNNNCTC 2 cut(s) 1191, 1223
BpmI CTGGAG 5 cut(s) 632, 885, 1006, 1382, 1400
BpuEI CTTGAG 3 cut(s) 77, 377, 392
BpuMI CCSGG 1 cut(s) 963
BsaHI GRCGYC 1 cut(s) 701
BsaJI CCNNGG 3 cut(s) 392, 561, 1377
BsaWI WCCGGW 1 cut(s) 1570
Bsc4I CCNNNNNNNGG 6 cut(s) 398, 409, 562, 610, 782, 984
Bse118I RCCGGY 2 cut(s) 438, 629
Bse1I ACTGG 2 cut(s) 136, 1365
Bse3DI GCAATG 1 cut(s) 1029
BseAI TCCGGA 1 cut(s) 1570
BseBI CCWGG 7 cut(s) 33, 399, 505, 605, 864, 1278, 1379
BseDI CCNNGG 3 cut(s) 392, 561, 1377
BseGI GGATG 2 cut(s) 174, 1225
BseLI CCNNNNNNNGG 6 cut(s) 398, 409, 562, 610, 782, 984
BseMI GCAATG 1 cut(s) 1029
BseMII CTCAG 5 cut(s) 261, 633, 754, 1092, 1170
BseNI ACTGG 2 cut(s) 136, 1365
BseRI GAGGAG 2 cut(s) 374, 735
BseSI GKGCMC 2 cut(s) 591, 1187
BseXI GCAGC 4 cut(s) 646, 993, 1096, 1378
BseYI CCCAGC 1 cut(s) 413
BsiHKAI GWGCWC 2 cut(s) 591, 741
BsiHKCI CYCGRG 1 cut(s) 992
BsiSI CCGG 4 cut(s) 439, 630, 963, 1571
BslFI GGGAC 1 cut(s) 1422
BslI CCNNNNNNNGG 6 cut(s) 398, 409, 562, 610, 782, 984
BsmAI GTCTC 2 cut(s) 376, 838
BsmFI GGGAC 1 cut(s) 1422
BsoBI CYCGRG 1 cut(s) 992
Bsp120I GGGCCC 1 cut(s) 1183
Bsp1286I GDGCHC 4 cut(s) 591, 741, 907, 1187
Bsp13I TCCGGA 1 cut(s) 1570
Bsp1407I TGTACA 1 cut(s) 420
BspACI CCGC 6 cut(s) 484, 695, 698, 725, 1348, 1527
BspCNI CTCAG 5 cut(s) 260, 634, 753, 1091, 1171
BspEI TCCGGA 1 cut(s) 1570
BspHI TCATGA 1 cut(s) 1516
BspLI GGNNCC 2 cut(s) 960, 1185
BspMAI CTGCAG 1 cut(s) 983
BspMI ACCTGC 1 cut(s) 886
BspPI GGATC 4 cut(s) 515, 953, 966, 976
BsrBI CCGCTC 1 cut(s) 725
BsrDI GCAATG 1 cut(s) 1029
BsrFI RCCGGY 2 cut(s) 438, 629
BsrGI TGTACA 1 cut(s) 420
BsrI ACTGG 2 cut(s) 136, 1365
BssAI RCCGGY 2 cut(s) 438, 629
BssECI CCNNGG 3 cut(s) 392, 561, 1377
BssNI GRCGYC 1 cut(s) 701
Bst2UI CCWGG 7 cut(s) 33, 399, 505, 605, 864, 1278, 1379
Bst4CI ACNGT 6 cut(s) 427, 655, 919, 1413, 1511, 1568
BstACI GRCGYC 1 cut(s) 701
BstAPI GCANNNNNTGC 1 cut(s) 668
BstAUI TGTACA 1 cut(s) 420
BstC8I GCNNGC 6 cut(s) 54, 404, 618, 627, 710, 1352
BstDEI CTNAG 7 cut(s) 191, 247, 642, 740, 1078, 1179, 1235
BstDSI CCRYGG 1 cut(s) 561
BstEII GGTNACC 1 cut(s) 131
BstF5I GGATG 2 cut(s) 174, 1225
BstHHI GCGC 2 cut(s) 444, 795
BstMAI GTCTC 2 cut(s) 376, 838
BstMWI GCNNNNNNNGC 8 cut(s) 403, 542, 605, 622, 626, 668, 714, 1118
BstNI CCWGG 7 cut(s) 33, 399, 505, 605, 864, 1278, 1379
BstNSI RCATGY 1 cut(s) 620
BstPAI GACNNNNGTC 1 cut(s) 1566
BstPI GGTNACC 1 cut(s) 131
BstSCI CCNGG 8 cut(s) 31, 397, 503, 603, 862, 961, 1276, 1377
BstSFI CTRYAG 1 cut(s) 979
BstSLI GKGCMC 2 cut(s) 591, 1187
BstV1I GCAGC 4 cut(s) 646, 993, 1096, 1378
BstX2I RGATCY 4 cut(s) 178, 958, 1448, 1667
BstYI RGATCY 4 cut(s) 178, 958, 1448, 1667
BtgI CCRYGG 1 cut(s) 561
BtrI CACGTC 1 cut(s) 489
BtsCI GGATG 2 cut(s) 174, 1225
BtsIMutI CAGTG 2 cut(s) 294, 1358
BveI ACCTGC 1 cut(s) 886
Cac8I GCNNGC 6 cut(s) 54, 404, 618, 627, 710, 1352
CaiI CAGNNNCTG 1 cut(s) 610
CciI TCATGA 1 cut(s) 1516
CfoI GCGC 2 cut(s) 444, 795
Cfr10I RCCGGY 2 cut(s) 438, 629
CseI GACGC 1 cut(s) 690
Csp6I GTAC 2 cut(s) 421, 1589
CviAII CATG 6 cut(s) 270, 298, 302, 593, 617, 1517
CviQI GTAC 2 cut(s) 421, 1589
DdeI CTNAG 7 cut(s) 191, 247, 642, 740, 1078, 1179, 1235
EciI GGCGGA 2 cut(s) 499, 1542
Eco24I GRGCYC 2 cut(s) 907, 1187
Eco47I GGWCC 4 cut(s) 501, 860, 915, 1292
Eco57I CTGAAG 4 cut(s) 195, 924, 1034, 1658
Eco88I CYCGRG 1 cut(s) 992
Eco91I GGTNACC 1 cut(s) 131
EcoO109I RGGNCCY 4 cut(s) 473, 543, 1183, 1292
EcoO65I GGTNACC 1 cut(s) 131
EcoRII CCWGG 7 cut(s) 31, 397, 503, 603, 862, 1276, 1377
EcoT22I ATGCAT 1 cut(s) 303
EcoT38I GRGCYC 2 cut(s) 907, 1187
FaeI CATG 6 cut(s) 273, 301, 305, 596, 620, 1520
FalI AAGNNNNNCTT 4 cut(s) 86, 118, 998, 1030
FaqI GGGAC 1 cut(s) 1422
FatI CATG 6 cut(s) 269, 297, 301, 592, 616, 1516
FbaI TGATCA 1 cut(s) 1087
Fnu4HI GCNGC 6 cut(s) 660, 696, 699, 982, 1110, 1367
FokI GGATG 2 cut(s) 181, 1212
FriOI GRGCYC 2 cut(s) 907, 1187
Fsp4HI GCNGC 6 cut(s) 660, 696, 699, 982, 1110, 1367
FspBI CTAG 2 cut(s) 705, 1154
FspI TGCGCA 1 cut(s) 794
GlaI GCGC 2 cut(s) 443, 794
GluI GCNGC 6 cut(s) 660, 696, 699, 982, 1110, 1367
GsaI CCCAGC 1 cut(s) 417
GsuI CTGGAG 5 cut(s) 632, 885, 1006, 1382, 1400
HapII CCGG 4 cut(s) 439, 630, 963, 1571
HgaI GACGC 1 cut(s) 690
HhaI GCGC 2 cut(s) 444, 795
Hin1I GRCGYC 1 cut(s) 701
Hin1II CATG 6 cut(s) 273, 301, 305, 596, 620, 1520
Hin6I GCGC 2 cut(s) 442, 793
HinP1I GCGC 2 cut(s) 442, 793
HincII GTYRAC 1 cut(s) 1507
HindII GTYRAC 1 cut(s) 1507
HindIII AAGCTT 3 cut(s) 1012, 1385, 1610
HinfI GANTC 3 cut(s) 1037, 1530, 1555
HpaII CCGG 4 cut(s) 439, 630, 963, 1571
HphI GGTGA 4 cut(s) 47, 286, 377, 1277
Hpy166II GTNNAC 7 cut(s) 141, 294, 423, 589, 915, 1507, 1655
Hpy188I TCNGA 6 cut(s) 151, 527, 643, 667, 743, 1638
Hpy188III TCNNGA 9 cut(s) 336, 430, 499, 611, 994, 1080, 1517, 1559, 1571
Hpy8I GTNNAC 7 cut(s) 141, 294, 423, 589, 915, 1507, 1655
Hpy99I CGWCG 2 cut(s) 541, 587
HpyAV CCTTC 4 cut(s) 93, 248, 1111, 1517
HpyCH4III ACNGT 6 cut(s) 427, 655, 919, 1413, 1511, 1568
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 8 cut(s) 52, 301, 589, 620, 662, 981, 1034, 1060
HpyF10VI GCNNNNNNNGC 8 cut(s) 403, 542, 605, 622, 626, 668, 714, 1118
HpyF3I CTNAG 7 cut(s) 191, 247, 642, 740, 1078, 1179, 1235
HpySE526I ACGT 1 cut(s) 488
Hsp92I GRCGYC 1 cut(s) 701
Hsp92II CATG 6 cut(s) 273, 301, 305, 596, 620, 1520
HspAI GCGC 2 cut(s) 442, 793
Kpn2I TCCGGA 1 cut(s) 1570
Ksp22I TGATCA 1 cut(s) 1087
LmnI GCTCC 4 cut(s) 613, 722, 1363, 1573
Lsp1109I GCAGC 4 cut(s) 646, 993, 1096, 1378
LweI GCATC 2 cut(s) 658, 671
MaeI CTAG 2 cut(s) 705, 1154
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 3 cut(s) 131, 349, 1174
MbiI CCGCTC 1 cut(s) 725
MboII GAAGA 6 cut(s) 50, 188, 930, 1305, 1532, 1628
MflI RGATCY 4 cut(s) 178, 958, 1448, 1667
MhlI GDGCHC 4 cut(s) 591, 741, 907, 1187
MluCI AATT 8 cut(s) 152, 521, 675, 779, 898, 1123, 1339, 1460
MlyI GAGTC 3 cut(s) 1046, 1539, 1549
MmeI TCCRAC 3 cut(s) 199, 433, 580
Mph1103I ATGCAT 1 cut(s) 303
MroI TCCGGA 1 cut(s) 1570
MroXI GAANNNNTTC 1 cut(s) 1272
MseI TTAA 1 cut(s) 1395
MslI CAYNNNNRTG 2 cut(s) 270, 1475
MspA1I CMGCKG 1 cut(s) 984
MspI CCGG 4 cut(s) 439, 630, 963, 1571
MspR9I CCNGG 8 cut(s) 33, 399, 505, 605, 864, 963, 1278, 1379
MvaI CCWGG 7 cut(s) 33, 399, 505, 605, 864, 1278, 1379
MwoI GCNNNNNNNGC 8 cut(s) 403, 542, 605, 622, 626, 668, 714, 1118
NciI CCSGG 1 cut(s) 963
NlaIII CATG 6 cut(s) 273, 301, 305, 596, 620, 1520
NlaIV GGNNCC 2 cut(s) 960, 1185
NmeAIII GCCGAG 1 cut(s) 417
NsbI TGCGCA 1 cut(s) 794
NsiI ATGCAT 1 cut(s) 303
NspI RCATGY 1 cut(s) 620
PaeI GCATGC 1 cut(s) 620
PaeR7I CTCGAG 1 cut(s) 992
PagI TCATGA 1 cut(s) 1516
PdmI GAANNNNTTC 1 cut(s) 1272
PflMI CCANNNNNTGG 2 cut(s) 562, 610
PfoI TCCNGGA 1 cut(s) 961
PkrI GCNGC 6 cut(s) 661, 697, 700, 983, 1111, 1368
PleI GAGTC 3 cut(s) 1045, 1538, 1549
PpsI GAGTC 3 cut(s) 1045, 1538, 1549
PpuMI RGGWCCY 1 cut(s) 1292
PshAI GACNNNNGTC 1 cut(s) 1566
Psp5II RGGWCCY 1 cut(s) 1292
Psp6I CCWGG 7 cut(s) 31, 397, 503, 603, 862, 1276, 1377
PspEI GGTNACC 1 cut(s) 131
PspFI CCCAGC 1 cut(s) 413
PspGI CCWGG 7 cut(s) 31, 397, 503, 603, 862, 1276, 1377
PspN4I GGNNCC 2 cut(s) 960, 1185
PspOMI GGGCCC 1 cut(s) 1183
PspPPI RGGWCCY 1 cut(s) 1292
PstI CTGCAG 1 cut(s) 983
PstNI CAGNNNCTG 1 cut(s) 610
PsuI RGATCY 4 cut(s) 178, 958, 1448, 1667
PvuII CAGCTG 1 cut(s) 984
RsaI GTAC 2 cut(s) 422, 1590
RsaNI GTAC 2 cut(s) 421, 1589
RseI CAYNNNNRTG 2 cut(s) 270, 1475
SaqAI TTAA 1 cut(s) 1395
SatI GCNGC 6 cut(s) 660, 696, 699, 982, 1110, 1367
SchI GAGTC 3 cut(s) 1046, 1539, 1549
ScrFI CCNGG 8 cut(s) 33, 399, 505, 605, 864, 963, 1278, 1379
SduI GDGCHC 4 cut(s) 591, 741, 907, 1187
SfaNI GCATC 2 cut(s) 658, 671
SfcI CTRYAG 1 cut(s) 979
Sfr274I CTCGAG 1 cut(s) 992
SinI GGWCC 4 cut(s) 501, 860, 915, 1292
SlaI CTCGAG 1 cut(s) 992
SmiMI CAYNNNNRTG 2 cut(s) 270, 1475
SmlI CTYRAG 4 cut(s) 92, 356, 407, 992
SmoI CTYRAG 4 cut(s) 92, 356, 407, 992
SphI GCATGC 1 cut(s) 620
Sse9I AATT 8 cut(s) 152, 521, 675, 779, 898, 1123, 1339, 1460
SsiI CCGC 6 cut(s) 484, 695, 698, 725, 1348, 1527
SspMI CTAG 2 cut(s) 705, 1154
StyD4I CCNGG 8 cut(s) 31, 397, 503, 603, 862, 961, 1276, 1377
TaaI ACNGT 6 cut(s) 427, 655, 919, 1413, 1511, 1568
TaiI ACGT 1 cut(s) 491
TaqI TCGA 2 cut(s) 993, 1428
TasI AATT 8 cut(s) 152, 521, 675, 779, 898, 1123, 1339, 1460
TatI WGTACW 1 cut(s) 420
TauI GCSGC 2 cut(s) 698, 701
Tru1I TTAA 1 cut(s) 1395
Tru9I TTAA 1 cut(s) 1395
TscAI CASTG 2 cut(s) 294, 1365
TseI GCWGC 4 cut(s) 659, 981, 1109, 1366
TspDTI ATGAA 5 cut(s) 258, 609, 1164, 1459, 1533
TspGWI ACGGA 1 cut(s) 1077
TspRI CASTG 2 cut(s) 294, 1365
Van91I CCANNNNNTGG 2 cut(s) 562, 610
VneI GTGCAC 1 cut(s) 587
VpaK11BI GGWCC 4 cut(s) 501, 860, 915, 1292
XapI RAATTY 1 cut(s) 521
XceI RCATGY 1 cut(s) 620
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XhoI CTCGAG 1 cut(s) 992
XmnI GAANNNNTTC 1 cut(s) 1272
XspI CTAG 2 cut(s) 705, 1154
Zsp2I ATGCAT 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.