AT1G05930

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000766
Physical Location & Seq
Forward (+)
1799418 .. 1800933
1516 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05930.1

Sequence Viewer

Length: 957 bp
ATGACGACAAACGACGATGATCACGCGGCTGAGAGAAACACGATGTCCATGAACTATGAATTGGCGGCTACGTTGAGTGATGAAGAACAACGCGCAAGAAAAGGAAAAGCCAAGATTGTCTGTAAAGAAGAAGACCACGTCTTTATCAAAAAGAAAGAAAAATACGAGGAAGAGAGCGAGAAGAGGGAATTCTTCAGTCATGTCCCCAGAAAGATAAGGCCTGCGCTTAGATACCCACAACCTAACTTTGAAAACCCTAATGGGGCTTCTTCTTCTTTAAACTTACCCTTCGAAGAAGACTACTACATGGCTGAGTACTACAAGAAGACGGAGACCATAAACCCACCTAACCCTTATCACCAATGGTCTCCTTCTTCGTTCTTAACGGAGTACACACACCCTAGGATGCTTGAGGTGTTACATCGCTGTGGATTCAACAGACCAGTGGTAACTTGTTATTCAAGGACGGCCAGGGAGATGCGGTGGTGGCTTCGCCAGGTGATGAAAGACATGAGAGCAGAAGACCTAACACTTATCTTAGAGAAGACTCTGTCCACAACTGATGTGATCACAACGACACATGGCCGTTTCTCAATGCATTTCAACAGATTAATCAGTAACGACTTCTTGAAGCCTGAGGAGCGGAGTATCCTAGAAGAAGATACCTACAATGATGAGACGATGGGAGTTGGAGCCATTCTCGTGGATCAAAGGTCTCAAAAGTGGAGTGTGATTTTGAAGAGATGGGGCCAGAATTACTTTCTGAGTTGTGGCTGGAACGACGTTGTTAAGGCTAATAAATTGAAAGCTGGCGACGACATCTGTCTTTGGGCGTTTAGGTGCGATGGAGTCCTCTGTTTTGCCATGAGGCAGTGGCGTGGAATCCTCTGTTTTGCTCTTGTTCCTCCTCTTACATTGAGACAGAGTAGCTCTTCCAATGCTCGCCGTCTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

318

Amino Acids

37.36

Weight (kDa)

8.01

Isoelectric Point (pI)

55.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 158 - 264 1.7e-38 B3 domain-containing protein At2g31720-like
B3 PF02362 225 - 274 1.7e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 643
AccII CGCG 2 cut(s) 26, 93
AciI CCGC 4 cut(s) 26, 65, 481, 643
AclWI GGATC 1 cut(s) 714
AcoI YGGCCR 2 cut(s) 468, 583
AcsI RAATTY 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 178
AfaI GTAC 2 cut(s) 317, 392
AfiI CCNNNNNNNGG 1 cut(s) 262
AgsI TTSAA 7 cut(s) 251, 436, 462, 604, 631, 739, 805
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 2 cut(s) 470, 495
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AluBI AGCT 2 cut(s) 809, 930
AluI AGCT 2 cut(s) 809, 930
Alw26I GTCTC 6 cut(s) 326, 372, 671, 720, 913, 953
AlwI GGATC 1 cut(s) 714
AoxI GGCC 4 cut(s) 218, 468, 583, 748
ApoI RAATTY 1 cut(s) 188
AseI ATTAAT 1 cut(s) 611
AspA2I CCTAGG 1 cut(s) 401
AspLEI GCGC 2 cut(s) 95, 226
AspS9I GGNCC 1 cut(s) 748
AsuHPI GGTGA 2 cut(s) 350, 511
AsuII TTCGAA 1 cut(s) 291
AvrII CCTAGG 1 cut(s) 401
AxyI CCTNAGG 1 cut(s) 636
BauI CACGAG 1 cut(s) 701
BbsI GAAGAC 5 cut(s) 138, 303, 332, 528, 551
BccI CCATC 3 cut(s) 676, 738, 839
BceAI ACGGC 3 cut(s) 483, 570, 930
BciT130I CCWGG 2 cut(s) 472, 497
BciVI GTATCC 1 cut(s) 659
BclI TGATCA 2 cut(s) 19, 567
BcoDI GTCTC 6 cut(s) 326, 372, 671, 720, 913, 953
BfaI CTAG 2 cut(s) 402, 653
BfuI GTATCC 1 cut(s) 659
BisI GCNGC 2 cut(s) 27, 66
BlnI CCTAGG 1 cut(s) 401
BlsI GCNGC 2 cut(s) 28, 67
BmcAI AGTACT 1 cut(s) 317
Bme1390I CCNGG 2 cut(s) 472, 497
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 748
BmiI GGNNCC 2 cut(s) 694, 749
BmrFI CCNGG 2 cut(s) 472, 497
BmsI GCATC 2 cut(s) 396, 468
BoxI GACNNNNGTC 1 cut(s) 822
BpiI GAAGAC 5 cut(s) 138, 303, 332, 528, 551
BplI GAGNNNNNCTC 2 cut(s) 684, 716
Bpu14I TTCGAA 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 431
BsaI GGTCTC 3 cut(s) 326, 372, 720
BsaJI CCNNGG 2 cut(s) 401, 471
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 443
Bse21I CCTNAGG 1 cut(s) 636
BseBI CCWGG 2 cut(s) 472, 497
BseDI CCNNGG 2 cut(s) 401, 471
BseGI GGATG 1 cut(s) 411
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMII CTCAG 4 cut(s) 21, 303, 627, 755
BseNI ACTGG 1 cut(s) 443
BseRI GAGGAG 2 cut(s) 653, 897
Bsh1236I CGCG 2 cut(s) 26, 93
BshFI GGCC 4 cut(s) 220, 470, 585, 750
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 6 cut(s) 326, 372, 671, 720, 913, 953
BsmBI CGTCTC 2 cut(s) 671, 953
BsmFI GGGAC 1 cut(s) 188
BsnI GGCC 4 cut(s) 220, 470, 585, 750
Bso31I GGTCTC 3 cut(s) 326, 372, 720
Bsp119I TTCGAA 1 cut(s) 291
Bsp143I GATC 3 cut(s) 19, 567, 706
BspACI CCGC 4 cut(s) 26, 65, 481, 643
BspANI GGCC 4 cut(s) 220, 470, 585, 750
BspCNI CTCAG 4 cut(s) 22, 304, 628, 756
BspFNI CGCG 2 cut(s) 26, 93
BspLI GGNNCC 2 cut(s) 694, 749
BspPI GGATC 1 cut(s) 714
BspQI GCTCTTC 1 cut(s) 937
BspT104I TTCGAA 1 cut(s) 291
BspTNI GGTCTC 3 cut(s) 326, 372, 720
BsrBI CCGCTC 1 cut(s) 643
BsrI ACTGG 1 cut(s) 443
BssECI CCNNGG 2 cut(s) 401, 471
BssMI GATC 3 cut(s) 19, 567, 706
BssSI CACGAG 1 cut(s) 701
BssT1I CCWWGG 1 cut(s) 401
Bst2BI CACGAG 1 cut(s) 701
Bst2UI CCWGG 2 cut(s) 472, 497
Bst6I CTCTTC 4 cut(s) 165, 176, 734, 937
BstBI TTCGAA 1 cut(s) 291
BstC8I GCNNGC 3 cut(s) 222, 811, 943
BstDEI CTNAG 6 cut(s) 30, 227, 312, 538, 636, 764
BstF5I GGATG 1 cut(s) 411
BstFNI CGCG 2 cut(s) 26, 93
BstHHI GCGC 2 cut(s) 95, 226
BstKTI GATC 3 cut(s) 22, 570, 709
BstMAI GTCTC 6 cut(s) 326, 372, 671, 720, 913, 953
BstMBI GATC 3 cut(s) 19, 567, 706
BstMWI GCNNNNNNNGC 3 cut(s) 487, 640, 951
BstNI CCWGG 2 cut(s) 472, 497
BstPAI GACNNNNGTC 1 cut(s) 822
BstSCI CCNGG 2 cut(s) 470, 495
BstUI CGCG 2 cut(s) 26, 93
BstV2I GAAGAC 5 cut(s) 138, 303, 332, 528, 551
BstXI CCANNNNNNTGG 1 cut(s) 703
Bsu36I CCTNAGG 1 cut(s) 636
BsuI GTATCC 1 cut(s) 659
BsuRI GGCC 4 cut(s) 220, 470, 585, 750
BtgZI GCGATG 2 cut(s) 407, 858
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 1 cut(s) 411
BtsI GCAGTG 1 cut(s) 878
BtsIMutI CAGTG 2 cut(s) 450, 878
Cac8I GCNNGC 3 cut(s) 222, 811, 943
CfoI GCGC 2 cut(s) 95, 226
Cfr13I GGNCC 1 cut(s) 748
Csp6I GTAC 2 cut(s) 316, 391
CviAII CATG 6 cut(s) 49, 200, 307, 511, 581, 865
CviQI GTAC 2 cut(s) 316, 391
DdeI CTNAG 6 cut(s) 30, 227, 312, 538, 636, 764
DpnI GATC 3 cut(s) 21, 569, 708
DpnII GATC 3 cut(s) 19, 567, 706
DraI TTTAAA 1 cut(s) 279
EaeI YGGCCR 2 cut(s) 468, 583
Eam1104I CTCTTC 4 cut(s) 165, 176, 734, 937
EarI CTCTTC 4 cut(s) 165, 176, 734, 937
Eco130I CCWWGG 1 cut(s) 401
Eco147I AGGCCT 1 cut(s) 220
Eco31I GGTCTC 3 cut(s) 326, 372, 720
Eco57I CTGAAG 1 cut(s) 178
Eco81I CCTNAGG 1 cut(s) 636
EcoRI GAATTC 1 cut(s) 188
EcoRII CCWGG 2 cut(s) 470, 495
EcoT14I CCWWGG 1 cut(s) 401
EcoT22I ATGCAT 1 cut(s) 600
ErhI CCWWGG 1 cut(s) 401
Esp3I CGTCTC 2 cut(s) 671, 953
FaeI CATG 6 cut(s) 52, 203, 310, 514, 584, 868
FaiI YATR 8 cut(s) 50, 57, 201, 308, 338, 512, 582, 866
FaqI GGGAC 1 cut(s) 188
FatI CATG 6 cut(s) 48, 199, 306, 510, 580, 864
FbaI TGATCA 2 cut(s) 19, 567
Fnu4HI GCNGC 2 cut(s) 27, 66
FokI GGATG 1 cut(s) 418
Fsp4HI GCNGC 2 cut(s) 27, 66
FspBI CTAG 2 cut(s) 402, 653
GlaI GCGC 2 cut(s) 94, 225
GluI GCNGC 2 cut(s) 27, 66
HaeIII GGCC 4 cut(s) 220, 470, 585, 750
HhaI GCGC 2 cut(s) 95, 226
Hin1II CATG 6 cut(s) 52, 203, 310, 514, 584, 868
Hin6I GCGC 2 cut(s) 93, 224
HinP1I GCGC 2 cut(s) 93, 224
HinfI GANTC 4 cut(s) 432, 547, 849, 882
HphI GGTGA 2 cut(s) 350, 511
Hpy166II GTNNAC 2 cut(s) 393, 555
Hpy188I TCNGA 1 cut(s) 765
Hpy188III TCNNGA 1 cut(s) 628
Hpy8I GTNNAC 2 cut(s) 393, 555
Hpy99I CGWCG 3 cut(s) 17, 785, 818
HpyAV CCTTC 2 cut(s) 298, 381
HpyCH4IV ACGT 3 cut(s) 71, 138, 783
HpyCH4V TGCA 1 cut(s) 598
HpyF10VI GCNNNNNNNGC 3 cut(s) 487, 640, 951
HpyF3I CTNAG 6 cut(s) 30, 227, 312, 538, 636, 764
HpySE526I ACGT 3 cut(s) 71, 138, 783
Hsp92II CATG 6 cut(s) 52, 203, 310, 514, 584, 868
HspAI GCGC 2 cut(s) 93, 224
Ksp22I TGATCA 2 cut(s) 19, 567
Kzo9I GATC 3 cut(s) 19, 567, 706
LguI GCTCTTC 1 cut(s) 937
LmnI GCTCC 2 cut(s) 640, 692
LweI GCATC 2 cut(s) 396, 468
MaeI CTAG 2 cut(s) 402, 653
MaeII ACGT 3 cut(s) 71, 138, 783
MaeIII GTNAC 3 cut(s) 417, 448, 617
MalI GATC 3 cut(s) 21, 569, 708
MbiI CCGCTC 1 cut(s) 643
MboI GATC 3 cut(s) 19, 567, 706
MluCI AATT 4 cut(s) 59, 188, 754, 800
MlyI GAGTC 2 cut(s) 541, 858
MmeI TCCRAC 1 cut(s) 670
MnlI CCTC 9 cut(s) 160, 177, 406, 631, 861, 863, 896, 915, 918
Mph1103I ATGCAT 1 cut(s) 600
MseI TTAA 4 cut(s) 278, 383, 611, 789
MslI CAYNNNNRTG 2 cut(s) 426, 701
MspR9I CCNGG 2 cut(s) 472, 497
MvaI CCWGG 2 cut(s) 472, 497
MvnI CGCG 2 cut(s) 26, 93
MwoI GCNNNNNNNGC 3 cut(s) 487, 640, 951
NdeII GATC 3 cut(s) 19, 567, 706
NlaIII CATG 6 cut(s) 52, 203, 310, 514, 584, 868
NlaIV GGNNCC 2 cut(s) 694, 749
NsiI ATGCAT 1 cut(s) 600
NspV TTCGAA 1 cut(s) 291
PceI AGGCCT 1 cut(s) 220
PciSI GCTCTTC 1 cut(s) 937
PfeI GAWTC 2 cut(s) 432, 882
PflFI GACNNNGTC 2 cut(s) 137, 550
PkrI GCNGC 2 cut(s) 28, 67
PleI GAGTC 2 cut(s) 541, 857
PpsI GAGTC 2 cut(s) 541, 857
PshAI GACNNNNGTC 1 cut(s) 822
PshBI ATTAAT 1 cut(s) 611
Psp6I CCWGG 2 cut(s) 470, 495
PspGI CCWGG 2 cut(s) 470, 495
PspN4I GGNNCC 2 cut(s) 694, 749
PspPI GGNCC 1 cut(s) 748
PsyI GACNNNGTC 2 cut(s) 137, 550
RsaI GTAC 2 cut(s) 317, 392
RsaNI GTAC 2 cut(s) 316, 391
RseI CAYNNNNRTG 2 cut(s) 426, 701
SapI GCTCTTC 1 cut(s) 937
SaqAI TTAA 4 cut(s) 278, 383, 611, 789
SatI GCNGC 2 cut(s) 27, 66
Sau3AI GATC 3 cut(s) 19, 567, 706
Sau96I GGNCC 1 cut(s) 748
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 2 cut(s) 541, 858
ScrFI CCNGG 2 cut(s) 472, 497
SfaNI GCATC 2 cut(s) 396, 468
SfuI TTCGAA 1 cut(s) 291
SmiMI CAYNNNNRTG 2 cut(s) 426, 701
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 4 cut(s) 59, 188, 754, 800
SseBI AGGCCT 1 cut(s) 220
SsiI CCGC 4 cut(s) 26, 65, 481, 643
SspMI CTAG 2 cut(s) 402, 653
StuI AGGCCT 1 cut(s) 220
StyD4I CCNGG 2 cut(s) 470, 495
StyI CCWWGG 1 cut(s) 401
TaiI ACGT 3 cut(s) 74, 141, 786
TaqI TCGA 1 cut(s) 291
TasI AATT 4 cut(s) 59, 188, 754, 800
TatI WGTACW 2 cut(s) 315, 390
TauI GCSGC 2 cut(s) 29, 68
TfiI GAWTC 2 cut(s) 432, 882
Tru1I TTAA 4 cut(s) 278, 383, 611, 789
Tru9I TTAA 4 cut(s) 278, 383, 611, 789
TscAI CASTG 2 cut(s) 450, 878
TspDTI ATGAA 4 cut(s) 65, 72, 96, 518
TspGWI ACGGA 2 cut(s) 344, 401
TspRI CASTG 2 cut(s) 450, 878
Tth111I GACNNNGTC 2 cut(s) 137, 550
VspI ATTAAT 1 cut(s) 611
XapI RAATTY 1 cut(s) 188
XmaJI CCTAGG 1 cut(s) 401
XspI CTAG 2 cut(s) 402, 653
ZrmI AGTACT 1 cut(s) 317
Zsp2I ATGCAT 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.