Rh1AG445200

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000766
Physical Location & Seq
Forward (+)
66545602 .. 66546720
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG445200.1

Sequence Viewer

Length: 1119 bp
ATGGCGCCGCTAGTGAGTATGGAAGACTTCATAGAAAAACAACTTCGACCTGGTTCAACCTTGAATATTTTGGGTCAGGTTGCTTGTGTTGCACGGGAGAAACTCGAGCAAAGGAGGAAGGAGAAAAGTATTAAAGTCAAAACTCAGATGGGTAGTAGTACTAGTAGTACCCCCAAGGTTAAAGAGCCCAATTATGTAGCTGAAACATATTCCAAAGAGACGGATGCTGATATGAACAAAAGAAAGAGTCGAGAGGACGATGTTGACCATGATGACAAGGGTTTTCAATTGAAACAACGGAAGACAATTTTTTTGCACGAGGAATTTGGTACTACCTCCAAGACTAAAGAACCAAATTCATCAATGGGTAAAGAATTTGAGGCGGATGCTTTATACTCGGTTGGCACAATAAGGAGAGACAGGTCTTTCCTTCGGACAAATTATATAACGAGCTCTGATTCAGGGTTTCAGGAAAAAAATGGGTTGACAAAGAAGAAGAGTATCGATGGTGGTGAGGATGGTAAAGCTTGGAGTTTGAAGCCCAAGCCGAAGAATATGAAGATTAAGAAGGAAAAGGGGTTGGGATTCAAGAGGCCTAAGGAGCATATGAGAGCTCCAAGATATAGTACTGATCCTAATGACTTGCCTGAAGACTTGAAAAGGAAGATTGGAACTTTGGAAGGTATTGAAAGTTTTGAAAAGGCTAAGATGACTATGGTGATAGAGAAGCGTCTGTTCGAGACTGATCTGACTGATAGCGCAAGCCGATTGTCAATGCCTCTGAAACAAATCAAACCGGAGAGTTTCTTGGACGAGGATGAGATAAAGTGTCTTGCATCTCAACAAGACTGGCCGGTTTCGGTTATAAGTCCCGAACTTAAGGACGAAAATCTAACTTTCAGGCAATGGAATATGAATAAGAATAACGGCAACGTTAGCTCAAGTTATGTGTTGAAAACAGGTTGGAACGAACTTCACAAGAAAAACGGTCTCCAAGTAAATGATGTAATCCAAGTTTGGTCATTTCGAGATGTGGGAAACAACCTTCACTTTGCTCTTGTTCTGGTTGAGAAGGCAACAGAGGGCGGTTTAAGTAGTGGTGGTGATGGGTGCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

372

Amino Acids

42.17

Weight (kDa)

8.99

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 236 - 325 2.7e-15 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 866
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 3 cut(s) 8, 383, 1086
AclI AACGTT 1 cut(s) 933
AclWI GGATC 1 cut(s) 626
AcoI YGGCCR 1 cut(s) 851
AcsI RAATTY 3 cut(s) 323, 355, 374
AcuI CTGAAG 1 cut(s) 669
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 160, 169, 331, 628
AflII CTTAAG 1 cut(s) 878
AhlI ACTAGT 1 cut(s) 161
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 5 cut(s) 200, 453, 527, 614, 939
AluI AGCT 5 cut(s) 200, 453, 527, 614, 939
Alw21I GWGCWC 2 cut(s) 455, 616
Alw26I GTCTC 4 cut(s) 212, 411, 734, 995
AlwI GGATC 1 cut(s) 626
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 2 cut(s) 593, 851
ApoI RAATTY 3 cut(s) 323, 355, 374
ArsI GACNNNNNNTTYG 2 cut(s) 295, 327
AspLEI GCGC 2 cut(s) 7, 761
AsuHPI GGTGA 3 cut(s) 524, 730, 1115
AvaI CYCGRG 1 cut(s) 104
AxyI CCTNAGG 1 cut(s) 597
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 3 cut(s) 189, 455, 616
BauI CACGAG 1 cut(s) 317
BbsI GAAGAC 3 cut(s) 30, 308, 657
Bbv12I GWGCWC 2 cut(s) 455, 616
BccI CCATC 4 cut(s) 142, 500, 512, 1100
BceAI ACGGC 1 cut(s) 943
BciT130I CCWGG 1 cut(s) 51
BcoDI GTCTC 4 cut(s) 212, 411, 734, 995
BcuI ACTAGT 1 cut(s) 161
BfaI CTAG 2 cut(s) 11, 162
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 878
BisI GCNGC 1 cut(s) 8
BlsI GCNGC 1 cut(s) 9
BmcAI AGTACT 2 cut(s) 160, 628
Bme1390I CCNGG 1 cut(s) 51
BmeT110I CYCGRG 1 cut(s) 104
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 3 cut(s) 214, 376, 845
BpiI GAAGAC 3 cut(s) 30, 308, 657
BpuEI CTTGAG 1 cut(s) 925
Bsa29I ATCGAT 1 cut(s) 504
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 995
BsaJI CCNNGG 1 cut(s) 174
BsaWI WCCGGW 1 cut(s) 796
Bse118I RCCGGY 1 cut(s) 853
Bse1I ACTGG 1 cut(s) 854
Bse21I CCTNAGG 1 cut(s) 597
Bse3DI GCAATG 1 cut(s) 911
BseBI CCWGG 1 cut(s) 51
BseCI ATCGAT 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 174
BseGI GGATG 4 cut(s) 229, 391, 523, 823
BseMI GCAATG 1 cut(s) 911
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 854
BshFI GGCC 2 cut(s) 595, 853
BshNI GGYRCC 1 cut(s) 4
BshVI ATCGAT 1 cut(s) 504
BsiHKAI GWGCWC 2 cut(s) 455, 616
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 2 cut(s) 797, 854
BslFI GGGAC 1 cut(s) 855
BsmAI GTCTC 4 cut(s) 212, 411, 734, 995
BsmBI CGTCTC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 855
BsnI GGCC 2 cut(s) 595, 853
Bso31I GGTCTC 1 cut(s) 995
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 3 cut(s) 189, 455, 616
Bsp143I GATC 2 cut(s) 631, 745
BspACI CCGC 3 cut(s) 8, 383, 1086
BspANI GGCC 2 cut(s) 595, 853
BspCNI CTCAG 1 cut(s) 157
BspDI ATCGAT 1 cut(s) 504
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 626
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 878
BspTNI GGTCTC 1 cut(s) 995
BsrDI GCAATG 1 cut(s) 911
BsrFI RCCGGY 1 cut(s) 853
BsrI ACTGG 1 cut(s) 854
BssAI RCCGGY 1 cut(s) 853
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 2 cut(s) 631, 745
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 174
Bst2BI CACGAG 1 cut(s) 317
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 989
Bst6I CTCTTC 1 cut(s) 491
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 878
BstC8I GCNNGC 1 cut(s) 763
BstDEI CTNAG 3 cut(s) 144, 597, 705
BstF5I GGATG 4 cut(s) 229, 391, 523, 823
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 761
BstKTI GATC 2 cut(s) 634, 748
BstMAI GTCTC 4 cut(s) 212, 411, 734, 995
BstMBI GATC 2 cut(s) 631, 745
BstMWI GCNNNNNNNGC 3 cut(s) 89, 601, 936
BstNI CCWGG 1 cut(s) 51
BstNSI RCATGY 1 cut(s) 1117
BstSCI CCNGG 1 cut(s) 49
BstV2I GAAGAC 3 cut(s) 30, 308, 657
Bsu15I ATCGAT 1 cut(s) 504
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 2 cut(s) 595, 853
BsuTUI ATCGAT 1 cut(s) 504
BtsCI GGATG 4 cut(s) 229, 391, 523, 823
Cac8I GCNNGC 1 cut(s) 763
CfoI GCGC 2 cut(s) 7, 761
Cfr10I RCCGGY 1 cut(s) 853
ClaI ATCGAT 1 cut(s) 504
CseI GACGC 1 cut(s) 719
CsiI ACCWGGT 1 cut(s) 49
Csp6I GTAC 4 cut(s) 159, 168, 330, 627
CviAII CATG 2 cut(s) 269, 1114
CviQI GTAC 4 cut(s) 159, 168, 330, 627
DdeI CTNAG 3 cut(s) 144, 597, 705
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 633, 747
DpnII GATC 2 cut(s) 631, 745
EaeI YGGCCR 1 cut(s) 851
Eam1104I CTCTTC 1 cut(s) 491
EarI CTCTTC 1 cut(s) 491
EciI GGCGGA 1 cut(s) 398
Ecl136II GAGCTC 2 cut(s) 453, 614
Eco130I CCWWGG 1 cut(s) 174
Eco147I AGGCCT 1 cut(s) 595
Eco24I GRGCYC 3 cut(s) 189, 455, 616
Eco31I GGTCTC 1 cut(s) 995
Eco53kI GAGCTC 2 cut(s) 453, 614
Eco57I CTGAAG 1 cut(s) 669
Eco81I CCTNAGG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 2 cut(s) 453, 614
EcoRII CCWGG 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 174
EcoT38I GRGCYC 3 cut(s) 189, 455, 616
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 174
Esp3I CGTCTC 1 cut(s) 212
FaeI CATG 2 cut(s) 272, 1117
FaqI GGGAC 1 cut(s) 855
FatI CATG 2 cut(s) 268, 1113
FauNDI CATATG 1 cut(s) 606
Fnu4HI GCNGC 1 cut(s) 8
FokI GGATG 4 cut(s) 236, 398, 530, 830
FriOI GRGCYC 3 cut(s) 189, 455, 616
Fsp4HI GCNGC 1 cut(s) 8
FspBI CTAG 2 cut(s) 11, 162
GlaI GCGC 2 cut(s) 6, 760
GluI GCNGC 1 cut(s) 8
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 595, 853
HapII CCGG 2 cut(s) 797, 854
HgaI GACGC 1 cut(s) 719
HhaI GCGC 2 cut(s) 7, 761
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 272, 1117
Hin6I GCGC 2 cut(s) 5, 759
HinP1I GCGC 2 cut(s) 5, 759
HincII GTYRAC 2 cut(s) 265, 486
HindII GTYRAC 2 cut(s) 265, 486
HindIII AAGCTT 1 cut(s) 525
HinfI GANTC 3 cut(s) 247, 458, 585
HpaII CCGG 2 cut(s) 797, 854
HphI GGTGA 3 cut(s) 524, 730, 1115
Hpy166II GTNNAC 2 cut(s) 265, 486
Hpy188I TCNGA 5 cut(s) 147, 435, 457, 750, 783
Hpy188III TCNNGA 6 cut(s) 251, 470, 589, 739, 872, 1028
Hpy8I GTNNAC 2 cut(s) 265, 486
HpyAV CCTTC 6 cut(s) 112, 440, 562, 674, 1055, 1066
HpyCH4III ACNGT 1 cut(s) 989
HpyCH4IV ACGT 1 cut(s) 933
HpyCH4V TGCA 4 cut(s) 92, 316, 836, 1113
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 601, 936
HpyF3I CTNAG 3 cut(s) 144, 597, 705
HpySE526I ACGT 1 cut(s) 933
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 272, 1117
HspAI GCGC 2 cut(s) 5, 759
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 2 cut(s) 631, 745
LmnI GCTCC 2 cut(s) 601, 619
LweI GCATC 3 cut(s) 214, 376, 845
MabI ACCWGGT 1 cut(s) 49
MaeI CTAG 2 cut(s) 11, 162
MaeII ACGT 1 cut(s) 933
MalI GATC 2 cut(s) 633, 747
MboI GATC 2 cut(s) 631, 745
MboII GAAGA 8 cut(s) 35, 313, 505, 508, 562, 571, 662, 676
MfeI CAATTG 1 cut(s) 287
MhlI GDGCHC 3 cut(s) 189, 455, 616
MluCI AATT 7 cut(s) 190, 287, 306, 323, 355, 374, 439
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 1 cut(s) 944
MseI TTAA 5 cut(s) 132, 180, 564, 879, 1091
MspCI CTTAAG 1 cut(s) 878
MspI CCGG 2 cut(s) 797, 854
MspR9I CCNGG 1 cut(s) 51
MunI CAATTG 1 cut(s) 287
MvaI CCWGG 1 cut(s) 51
MwoI GCNNNNNNNGC 3 cut(s) 89, 601, 936
NarI GGCGCC 1 cut(s) 5
NdeI CATATG 1 cut(s) 606
NdeII GATC 2 cut(s) 631, 745
NlaIII CATG 2 cut(s) 272, 1117
NlaIV GGNNCC 1 cut(s) 6
NspI RCATGY 1 cut(s) 1117
PaeR7I CTCGAG 1 cut(s) 104
PceI AGGCCT 1 cut(s) 595
PfeI GAWTC 2 cut(s) 458, 585
PkrI GCNGC 1 cut(s) 9
PleI GAGTC 1 cut(s) 255
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 255
PsiI TTATAA 1 cut(s) 866
Psp124BI GAGCTC 2 cut(s) 455, 616
Psp1406I AACGTT 1 cut(s) 933
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 1 cut(s) 6
PspXI VCTCGAGB 1 cut(s) 104
RsaI GTAC 4 cut(s) 160, 169, 331, 628
RsaNI GTAC 4 cut(s) 159, 168, 330, 627
SacI GAGCTC 2 cut(s) 455, 616
SaqAI TTAA 5 cut(s) 132, 180, 564, 879, 1091
SatI GCNGC 1 cut(s) 8
Sau3AI GATC 2 cut(s) 631, 745
ScaI AGTACT 2 cut(s) 160, 628
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 1 cut(s) 51
SduI GDGCHC 3 cut(s) 189, 455, 616
SexAI ACCWGGT 1 cut(s) 49
SfaNI GCATC 3 cut(s) 214, 376, 845
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 104
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 3 cut(s) 104, 878, 940
SmoI CTYRAG 3 cut(s) 104, 878, 940
SpeI ACTAGT 1 cut(s) 161
Sse9I AATT 7 cut(s) 190, 287, 306, 323, 355, 374, 439
SseBI AGGCCT 1 cut(s) 595
SsiI CCGC 3 cut(s) 8, 383, 1086
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 67
SspMI CTAG 2 cut(s) 11, 162
SstI GAGCTC 2 cut(s) 455, 616
StuI AGGCCT 1 cut(s) 595
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 1 cut(s) 989
TaiI ACGT 1 cut(s) 936
TaqI TCGA 6 cut(s) 46, 105, 250, 504, 738, 1027
TasI AATT 7 cut(s) 190, 287, 306, 323, 355, 374, 439
TatI WGTACW 2 cut(s) 158, 626
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 2 cut(s) 458, 585
Tru1I TTAA 5 cut(s) 132, 180, 564, 879, 1091
Tru9I TTAA 5 cut(s) 132, 180, 564, 879, 1091
TspDTI ATGAA 5 cut(s) 19, 248, 348, 572, 929
TspGWI ACGGA 2 cut(s) 236, 313
Vha464I CTTAAG 1 cut(s) 878
XapI RAATTY 3 cut(s) 323, 355, 374
XceI RCATGY 1 cut(s) 1117
XhoI CTCGAG 1 cut(s) 104
XspI CTAG 2 cut(s) 11, 162
ZrmI AGTACT 2 cut(s) 160, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.