AT1G32030

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000766
Physical Location & Seq
Forward (+)
11514594 .. 11515595
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32030.1

Sequence Viewer

Length: 1002 bp
ATGTCTACGTATTATAATGATCATCTCGCGGCTGCGGAGAAGGACATAAGCTCGAGTAATTTCTGTAATTTAGTGGATGCTTCCGTTATGTTATACGACGAAGAACACCGTGATCTTCTTCCAAAGAAAGTAAACTCCAGATGCCAAGCGAAGACAAAGAAACAAGAAAAAGAGAGTGACAAGAGATTCTTTGATCTCTTCCCTAAAAAAAGAAGAACTTCGGTTGTTACTGTAAGAAACCCTGAACAAAACCAACAAAATCTTCATAGGGTTTCTACTTCTTCATCGCTCCTCGACCTTAACACGATCCCCGGTGATTCTTCTGACCCACGTAACGAACTTCAAGGGCTTTTATCTTCTTCTTCATCATCGTTTCTTGGAGATTACGAAAGGAAAACCCCACAAAACGCTTCTTCTTCTTCAACTTTACTTGCTGAGTACAACACCGAGATGGCAAACCCACCAAACCCTAATTCTGAGACAAGCCTCAAGAGAAAGCATAGTGATAGTGAACCCAAGAAAGCAAAGACTCCTTATTCATGGAAAGGGAGAGAGGCGCCGGAGTGGCTGGTCAAGATGATGGGAACAATGCAGGGAAGCGAAGGCCCGAGGCTAATCTATGAGAAGACTCTGACCGAAACAGATGTCAAACCAGTCCAGAGCCGTCTCTTAATGCCTTTCAACACCCTAATTCGTAACGACTTTTTGACGCCTGTTGAGTTGAGAATCCTACTAGAATTAGAAGATGACACGGATGGAGTTGGAGCGACTCTAGTGGATCCATGGGAGGTAAAGTGGGGTGTGATTTTGAAGAAACGGGAGATGAAGAAATATTCAGGGAAAGGAAGCTTGAACTACGCTATAATATGCGGGTGGAACGATATCGTTGAGGCTAACGTATTGGAAAAAGACGATGACATCAGTATTTGGTCTTTCAGGCGAGGTAGAAATGGAATCCTTTCCTTTGCCCTTGTTCTTCCTCCTCCTCCTGACATGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

333

Amino Acids

37.7

Weight (kDa)

6.49

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 184 - 298 8.2e-40 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 15
AccB1I GGYRCC 1 cut(s) 556
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 29
AciI CCGC 3 cut(s) 29, 35, 870
AclWI GGATC 3 cut(s) 301, 773, 786
AcyI GRCGYC 2 cut(s) 557, 710
AfaI GTAC 1 cut(s) 440
AgsI TTSAA 5 cut(s) 344, 423, 682, 811, 853
AjuI GAANNNNNNNTTGG 4 cut(s) 246, 278, 457, 489
AluBI AGCT 2 cut(s) 51, 849
AluI AGCT 2 cut(s) 51, 849
Alw26I GTCTC 2 cut(s) 473, 671
AlwI GGATC 3 cut(s) 301, 773, 786
Ama87I CYCGRG 2 cut(s) 52, 607
AoxI GGCC 1 cut(s) 604
ApeKI GCWGC 1 cut(s) 32
ArsI GACNNNNNNTTYG 2 cut(s) 630, 662
Asp700I GAANNNNTTC 2 cut(s) 217, 958
AspLEI GCGC 1 cut(s) 559
AspS9I GGNCC 1 cut(s) 605
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 326
AvaI CYCGRG 2 cut(s) 52, 607
BamHI GGATCC 1 cut(s) 778
BanI GGYRCC 1 cut(s) 556
BbsI GAAGAC 2 cut(s) 158, 632
BbvI GCAGC 1 cut(s) 19
BccI CCATC 3 cut(s) 445, 574, 749
BceAI ACGGC 1 cut(s) 648
BclI TGATCA 1 cut(s) 19
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 2 cut(s) 473, 671
BfaI CTAG 2 cut(s) 734, 773
BfoI RGCGCY 1 cut(s) 560
BglI GCCNNNNNGGC 1 cut(s) 565
BisI GCNGC 2 cut(s) 30, 33
BlsI GCNGC 2 cut(s) 31, 34
Bme1390I CCNGG 1 cut(s) 312
BmeT110I CYCGRG 2 cut(s) 52, 607
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 2 cut(s) 558, 780
BmrFI CCNGG 1 cut(s) 312
BmsI GCATC 2 cut(s) 67, 131
BpiI GAAGAC 2 cut(s) 158, 632
BpmI CTGGAG 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 1 cut(s) 312
BsaAI YACGTR 2 cut(s) 9, 332
BsaHI GRCGYC 2 cut(s) 557, 710
BsaJI CCNNGG 3 cut(s) 310, 608, 782
BsaXI ACNNNNNCTCC 2 cut(s) 779, 809
Bse1I ACTGG 1 cut(s) 653
BseDI CCNNGG 3 cut(s) 310, 608, 782
BseGI GGATG 2 cut(s) 82, 760
BseMII CTCAG 2 cut(s) 426, 468
BseNI ACTGG 1 cut(s) 653
BseRI GAGGAG 3 cut(s) 281, 972, 975
BseXI GCAGC 1 cut(s) 19
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 606
BshNI GGYRCC 1 cut(s) 556
BsiHKCI CYCGRG 2 cut(s) 52, 607
BsiSI CCGG 2 cut(s) 312, 560
BsmAI GTCTC 2 cut(s) 473, 671
BsmBI CGTCTC 1 cut(s) 671
BsnI GGCC 1 cut(s) 606
BsoBI CYCGRG 2 cut(s) 52, 607
Bsp143I GATC 5 cut(s) 19, 112, 193, 306, 778
Bsp19I CCATGG 1 cut(s) 782
BspACI CCGC 3 cut(s) 29, 35, 870
BspANI GGCC 1 cut(s) 606
BspCNI CTCAG 2 cut(s) 427, 469
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 2 cut(s) 558, 780
BspPI GGATC 3 cut(s) 301, 773, 786
BspT107I GGYRCC 1 cut(s) 556
BsrI ACTGG 1 cut(s) 653
BssECI CCNNGG 3 cut(s) 310, 608, 782
BssMI GATC 5 cut(s) 19, 112, 193, 306, 778
BssNI GRCGYC 2 cut(s) 557, 710
BssT1I CCWWGG 1 cut(s) 782
Bst4CI ACNGT 2 cut(s) 110, 232
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 2 cut(s) 557, 710
BstBAI YACGTR 2 cut(s) 9, 332
BstDEI CTNAG 2 cut(s) 435, 477
BstDSI CCRYGG 1 cut(s) 782
BstF5I GGATG 2 cut(s) 82, 760
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 5 cut(s) 22, 115, 196, 309, 781
BstMAI GTCTC 2 cut(s) 473, 671
BstMBI GATC 5 cut(s) 19, 112, 193, 306, 778
BstMWI GCNNNNNNNGC 1 cut(s) 565
BstSCI CCNGG 1 cut(s) 310
BstSNI TACGTA 1 cut(s) 9
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 19
BstV2I GAAGAC 2 cut(s) 158, 632
BstX2I RGATCY 1 cut(s) 778
BstYI RGATCY 1 cut(s) 778
BsuRI GGCC 1 cut(s) 606
BtgI CCRYGG 1 cut(s) 782
BtgZI GCGATG 1 cut(s) 270
BtsCI GGATG 2 cut(s) 82, 760
CfoI GCGC 1 cut(s) 559
Cfr13I GGNCC 1 cut(s) 605
CseI GACGC 1 cut(s) 718
Csp6I GTAC 1 cut(s) 439
CviAII CATG 3 cut(s) 540, 783, 994
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 2 cut(s) 435, 477
DinI GGCGCC 1 cut(s) 558
DpnI GATC 5 cut(s) 21, 114, 195, 308, 780
DpnII GATC 5 cut(s) 19, 112, 193, 306, 778
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
Eco105I TACGTA 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 782
Eco32I GATATC 1 cut(s) 883
Eco88I CYCGRG 2 cut(s) 52, 607
EcoRV GATATC 1 cut(s) 883
EcoT14I CCWWGG 1 cut(s) 782
EgeI GGCGCC 1 cut(s) 558
EheI GGCGCC 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 782
Esp3I CGTCTC 1 cut(s) 671
FaeI CATG 3 cut(s) 543, 786, 997
FalI AAGNNNNNCTT 4 cut(s) 173, 205, 202, 234
FatI CATG 3 cut(s) 539, 782, 993
FauI CCCGC 1 cut(s) 863
FbaI TGATCA 1 cut(s) 19
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 30, 33
FokI GGATG 2 cut(s) 89, 767
Fsp4HI GCNGC 2 cut(s) 30, 33
FspBI CTAG 2 cut(s) 734, 773
GlaI GCGC 1 cut(s) 558
GluI GCNGC 2 cut(s) 30, 33
GsuI CTGGAG 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 1 cut(s) 606
HapII CCGG 2 cut(s) 312, 560
HgaI GACGC 1 cut(s) 718
HhaI GCGC 1 cut(s) 559
Hin1I GRCGYC 2 cut(s) 557, 710
Hin1II CATG 3 cut(s) 543, 786, 997
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HindIII AAGCTT 1 cut(s) 847
HinfI GANTC 7 cut(s) 186, 317, 529, 628, 726, 769, 954
HpaII CCGG 2 cut(s) 312, 560
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 3 cut(s) 6, 133, 512
Hpy188I TCNGA 3 cut(s) 325, 478, 633
Hpy188III TCNNGA 5 cut(s) 138, 490, 574, 658, 989
Hpy8I GTNNAC 3 cut(s) 6, 133, 512
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 34, 596
HpyCH4III ACNGT 2 cut(s) 110, 232
HpyCH4IV ACGT 3 cut(s) 8, 331, 897
HpyCH4V TGCA 1 cut(s) 592
HpyF10VI GCNNNNNNNGC 1 cut(s) 565
HpyF3I CTNAG 2 cut(s) 435, 477
HpySE526I ACGT 3 cut(s) 8, 331, 897
Hsp92I GRCGYC 2 cut(s) 557, 710
Hsp92II CATG 3 cut(s) 543, 786, 997
HspAI GCGC 1 cut(s) 557
KasI GGCGCC 1 cut(s) 556
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 5 cut(s) 19, 112, 193, 306, 778
LmnI GCTCC 2 cut(s) 294, 764
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 2 cut(s) 67, 131
MaeI CTAG 2 cut(s) 734, 773
MaeII ACGT 3 cut(s) 8, 331, 897
MaeIII GTNAC 4 cut(s) 176, 226, 332, 695
MalI GATC 5 cut(s) 21, 114, 195, 308, 780
MboI GATC 5 cut(s) 19, 112, 193, 306, 778
MflI RGATCY 1 cut(s) 778
MluCI AATT 5 cut(s) 58, 67, 472, 690, 737
Mly113I GGCGCC 1 cut(s) 557
MlyI GAGTC 3 cut(s) 523, 622, 763
MmeI TCCRAC 1 cut(s) 742
MroXI GAANNNNTTC 2 cut(s) 217, 958
MseI TTAA 2 cut(s) 300, 671
MslI CAYNNNNRTG 1 cut(s) 449
MspI CCGG 2 cut(s) 312, 560
MspR9I CCNGG 1 cut(s) 312
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 1 cut(s) 565
NarI GGCGCC 1 cut(s) 557
NciI CCSGG 1 cut(s) 312
NcoI CCATGG 1 cut(s) 782
NdeII GATC 5 cut(s) 19, 112, 193, 306, 778
NlaIII CATG 3 cut(s) 543, 786, 997
NlaIV GGNNCC 2 cut(s) 558, 780
NmuCI GTSAC 1 cut(s) 176
PaeR7I CTCGAG 1 cut(s) 52
PdmI GAANNNNTTC 2 cut(s) 217, 958
PfeI GAWTC 4 cut(s) 186, 317, 726, 954
PkrI GCNGC 2 cut(s) 31, 34
PleI GAGTC 3 cut(s) 523, 622, 763
PluTI GGCGCC 1 cut(s) 560
PpsI GAGTC 3 cut(s) 523, 622, 763
Ppu21I YACGTR 2 cut(s) 9, 332
PsiI TTATAA 1 cut(s) 15
PspN4I GGNNCC 2 cut(s) 558, 780
PspPI GGNCC 1 cut(s) 605
PspXI VCTCGAGB 1 cut(s) 52
PsuI RGATCY 1 cut(s) 778
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 2 cut(s) 300, 671
SatI GCNGC 2 cut(s) 30, 33
Sau3AI GATC 5 cut(s) 19, 112, 193, 306, 778
Sau96I GGNCC 1 cut(s) 605
SchI GAGTC 3 cut(s) 523, 622, 763
ScrFI CCNGG 1 cut(s) 312
SetI ASST 8 cut(s) 11, 53, 300, 334, 792, 851, 900, 946
SfaNI GCATC 2 cut(s) 67, 131
SfoI GGCGCC 1 cut(s) 558
Sfr274I CTCGAG 1 cut(s) 52
SlaI CTCGAG 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 2 cut(s) 52, 488
SmoI CTYRAG 2 cut(s) 52, 488
SnaBI TACGTA 1 cut(s) 9
Sse9I AATT 5 cut(s) 58, 67, 472, 690, 737
SsiI CCGC 3 cut(s) 29, 35, 870
SspDI GGCGCC 1 cut(s) 556
SspI AATATT 1 cut(s) 833
SspMI CTAG 2 cut(s) 734, 773
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 1 cut(s) 782
TaaI ACNGT 2 cut(s) 110, 232
TaiI ACGT 3 cut(s) 11, 334, 900
TaqI TCGA 2 cut(s) 53, 294
TaqII GACCGA 1 cut(s) 650
TasI AATT 5 cut(s) 58, 67, 472, 690, 737
TatI WGTACW 1 cut(s) 438
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 4 cut(s) 186, 317, 726, 954
Tru1I TTAA 2 cut(s) 300, 671
Tru9I TTAA 2 cut(s) 300, 671
TseFI GTSAC 1 cut(s) 176
TseI GCWGC 1 cut(s) 32
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 5 cut(s) 254, 273, 354, 528, 839
TspGWI ACGGA 2 cut(s) 73, 767
XhoI CTCGAG 1 cut(s) 52
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 2 cut(s) 217, 958
XspI CTAG 2 cut(s) 734, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.