Rroxscaffold_4G00304070

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000766
Physical Location & Seq
Forward (+)
24707462 .. 24708637
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00304070.1

Sequence Viewer

Length: 1176 bp
ATGGAGCTTATAAGTAAGCGAGATTTCGAGGGCAAGGATTTTGGTCCCAACTTGTCAAGTTTGGAGATGTTAGCAATGGTTACTGCTGTTGCTGCCAAGAAATTGAATCGAGAAAAGCAACAGAGGATGATGGGTACCTTCAACTCCAAGAAAGAAGAAAGTGAGGCATACCAATCAGTGTCGACTAAGCAAGAATTTCTGGTGGCAGAGAAGAGTGCTTTAAATAAGCCTCTGGTAATAACCTCGAAGAAAGGGAAGCCTACGGAAAAAGAGCCTGAAAATACCCTAGGAAGTTATAGTGGGTACCTTCAACTCCAAATGGGTACCTTAAACTCCAAGAAAGAACCAGAATATGTTGTAGAGAAAGAATCTGGTTCATTCATTCCGAATAAGCCAAGGGGATGGCAGCCCAAGAAAGAAAACGGTGTAAGAGAACAAAAAGTTATGAGCATAAAAAAGAATGGTTTGAAAAGAAAATTCCAAGATGAGCATGATTGGGAGAACAAGAAATTAGTACCCAATAGTACCGGCGACATGCCTGAAGAGTTCAAGAACGAGATTGAAACTTCCGGAAGGATTAAGGTGGAGTTGATGATTGAGAATAAATTGTTCAAGTCTGATGGTGTTGACAATGCTGCCCACGATCATGATTGGGATTTGGATTATGCATTTAAGAACAATTACATGAAGCACAAGGGCTTGATGAAAAGGCAGAAGAAAAAATTCGTACCAAATAGAAGTAGTACTCCTACTCATGACTTGCCTGAAGTGTTCAGGAAGAAGATTGAAACTTTGGGAGGGACTAAGGTGAACTTGGTGATTGAGAAGACATTGTACAAGTCTGACTTGGTACAGGGCGAAAACCGGTTGTCAATGCCTTTCAAACAAATCCGCTCCAAATTCTTGGAAGATGATGAAGTTAGAAGGCTTGCGAACTATGAAACCTTGACGGTCCCATTGATAGACACCATGCTACAAGAAAGAGAGATATGCTTGAGTCGGTGGAGCTTGCACACAAGCAAATGGTATGTGCTGAAAACTCAATGGTACAAGAAAATTGTTTTGAACAACGGACTTAGAAAAGGTGACTTGATCCAAGTTTGGTCATTCCGAGATGAGGTGAACAACCTTCGCTTGGCACTTGTTTTGGTTGAGAGAAAAGGCGAGGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

391

Amino Acids

45.43

Weight (kDa)

9.4

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 258 - 351 9e-13 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 11
Acc65I GGTACC 3 cut(s) 134, 303, 323
AccB1I GGYRCC 3 cut(s) 134, 303, 323
AccBSI CCGCTC 1 cut(s) 894
AccI GTMKAC 1 cut(s) 182
AccIII TCCGGA 1 cut(s) 569
AciI CCGC 1 cut(s) 892
AclWI GGATC 1 cut(s) 1087
AcsI RAATTY 4 cut(s) 194, 476, 722, 899
AcuI CTGAAG 2 cut(s) 561, 786
AfiI CCNNNNNNNGG 2 cut(s) 1117, 1135
AgeI ACCGGT 1 cut(s) 864
AluBI AGCT 2 cut(s) 7, 1008
AluI AGCT 2 cut(s) 7, 1008
AlwI GGATC 1 cut(s) 1087
Aor13HI TCCGGA 1 cut(s) 569
ApeKI GCWGC 3 cut(s) 92, 406, 635
ApoI RAATTY 4 cut(s) 194, 476, 722, 899
AsiGI ACCGGT 1 cut(s) 864
Asp700I GAANNNNTTC 1 cut(s) 722
Asp718I GGTACC 3 cut(s) 134, 303, 323
AspA2I CCTAGG 1 cut(s) 286
AspS9I GGNCC 2 cut(s) 44, 952
AsuHPI GGTGA 4 cut(s) 820, 829, 1097, 1132
AvaII GGWCC 2 cut(s) 44, 952
AvrII CCTAGG 1 cut(s) 286
BanI GGYRCC 3 cut(s) 134, 303, 323
BarI GAAGNNNNNNTAC 2 cut(s) 818, 850
BbsI GAAGAC 1 cut(s) 833
BbvI GCAGC 3 cut(s) 79, 418, 622
BccI CCATC 3 cut(s) 124, 396, 614
BfaI CTAG 1 cut(s) 287
BisI GCNGC 3 cut(s) 93, 407, 636
BlnI CCTAGG 1 cut(s) 286
BlsI GCNGC 3 cut(s) 94, 408, 637
BmcAI AGTACT 1 cut(s) 745
Bme18I GGWCC 2 cut(s) 44, 952
BmgT120I GGNCC 2 cut(s) 44, 952
BmiI GGNNCC 5 cut(s) 46, 136, 305, 325, 954
BpiI GAAGAC 1 cut(s) 833
BpuEI CTTGAG 1 cut(s) 1015
BsaJI CCNNGG 2 cut(s) 286, 395
BsaWI WCCGGW 2 cut(s) 569, 864
Bsc4I CCNNNNNNNGG 2 cut(s) 1117, 1135
Bse118I RCCGGY 2 cut(s) 527, 864
Bse3DI GCAATG 1 cut(s) 81
BseAI TCCGGA 1 cut(s) 569
BseDI CCNNGG 2 cut(s) 286, 395
BseGI GGATG 2 cut(s) 132, 407
BseLI CCNNNNNNNGG 2 cut(s) 1117, 1135
BseMI GCAATG 1 cut(s) 81
BseXI GCAGC 3 cut(s) 79, 418, 622
BshNI GGYRCC 3 cut(s) 134, 303, 323
BshTI ACCGGT 1 cut(s) 864
BsiSI CCGG 3 cut(s) 528, 570, 865
BslFI GGGAC 3 cut(s) 30, 814, 938
BslI CCNNNNNNNGG 2 cut(s) 1117, 1135
BsmFI GGGAC 3 cut(s) 30, 814, 938
Bsp13I TCCGGA 1 cut(s) 569
Bsp1407I TGTACA 1 cut(s) 834
Bsp143I GATC 2 cut(s) 643, 1092
BspACI CCGC 1 cut(s) 892
BspEI TCCGGA 1 cut(s) 569
BspHI TCATGA 2 cut(s) 646, 754
BspLI GGNNCC 5 cut(s) 46, 136, 305, 325, 954
BspPI GGATC 1 cut(s) 1087
BspT107I GGYRCC 3 cut(s) 134, 303, 323
BsrBI CCGCTC 1 cut(s) 894
BsrDI GCAATG 1 cut(s) 81
BsrFI RCCGGY 2 cut(s) 527, 864
BsrGI TGTACA 1 cut(s) 834
BssAI RCCGGY 2 cut(s) 527, 864
BssECI CCNNGG 2 cut(s) 286, 395
BssMI GATC 2 cut(s) 643, 1092
BssT1I CCWWGG 2 cut(s) 286, 395
Bst4CI ACNGT 2 cut(s) 425, 952
Bst6I CTCTTC 2 cut(s) 206, 537
BstAUI TGTACA 1 cut(s) 834
BstC8I GCNNGC 2 cut(s) 930, 1010
BstDEI CTNAG 3 cut(s) 186, 804, 1076
BstF5I GGATG 2 cut(s) 132, 407
BstKTI GATC 2 cut(s) 646, 1095
BstMBI GATC 2 cut(s) 643, 1092
BstMWI GCNNNNNNNGC 1 cut(s) 92
BstNSI RCATGY 1 cut(s) 538
BstV1I GCAGC 3 cut(s) 79, 418, 622
BstV2I GAAGAC 1 cut(s) 833
BstXI CCANNNNNNTGG 2 cut(s) 402, 904
BtsCI GGATG 2 cut(s) 132, 407
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 2 cut(s) 930, 1010
CciI TCATGA 2 cut(s) 646, 754
Cfr10I RCCGGY 2 cut(s) 527, 864
Cfr13I GGNCC 2 cut(s) 44, 952
CspAI ACCGGT 1 cut(s) 864
CviAII CATG 6 cut(s) 491, 535, 647, 685, 755, 970
CviJI RGCY 9 cut(s) 7, 229, 259, 274, 394, 409, 699, 928, 1008
CviKI_1 RGCY 9 cut(s) 7, 229, 259, 274, 394, 409, 699, 928, 1008
DdeI CTNAG 3 cut(s) 186, 804, 1076
DpnI GATC 2 cut(s) 645, 1094
DpnII GATC 2 cut(s) 643, 1092
DraI TTTAAA 1 cut(s) 222
Eam1104I CTCTTC 2 cut(s) 206, 537
EarI CTCTTC 2 cut(s) 206, 537
Eco130I CCWWGG 2 cut(s) 286, 395
Eco47I GGWCC 2 cut(s) 44, 952
Eco57I CTGAAG 2 cut(s) 561, 786
EcoT14I CCWWGG 2 cut(s) 286, 395
EcoT22I ATGCAT 1 cut(s) 670
ErhI CCWWGG 2 cut(s) 286, 395
FaeI CATG 6 cut(s) 494, 538, 650, 688, 758, 973
FalI AAGNNNNNCTT 4 cut(s) 797, 829, 830, 862
FaqI GGGAC 3 cut(s) 30, 814, 938
FatI CATG 6 cut(s) 490, 534, 646, 684, 754, 969
FblI GTMKAC 1 cut(s) 182
Fnu4HI GCNGC 3 cut(s) 93, 407, 636
FokI GGATG 2 cut(s) 139, 414
Fsp4HI GCNGC 3 cut(s) 93, 407, 636
FspBI CTAG 1 cut(s) 287
GluI GCNGC 3 cut(s) 93, 407, 636
HapII CCGG 3 cut(s) 528, 570, 865
Hin1II CATG 6 cut(s) 494, 538, 650, 688, 758, 973
HincII GTYRAC 2 cut(s) 183, 628
HindII GTYRAC 2 cut(s) 183, 628
HinfI GANTC 3 cut(s) 106, 368, 997
HpaII CCGG 3 cut(s) 528, 570, 865
HphI GGTGA 4 cut(s) 820, 829, 1097, 1132
Hpy166II GTNNAC 4 cut(s) 183, 628, 811, 1123
Hpy188I TCNGA 4 cut(s) 387, 619, 844, 1112
Hpy188III TCNNGA 6 cut(s) 110, 550, 570, 647, 755, 775
Hpy8I GTNNAC 4 cut(s) 183, 628, 811, 1123
HpyAV CCTTC 5 cut(s) 148, 317, 567, 918, 1139
HpyCH4III ACNGT 2 cut(s) 425, 952
HpyCH4V TGCA 2 cut(s) 668, 1012
HpyF10VI GCNNNNNNNGC 1 cut(s) 92
HpyF3I CTNAG 3 cut(s) 186, 804, 1076
Hsp92II CATG 6 cut(s) 494, 538, 650, 688, 758, 973
Kpn2I TCCGGA 1 cut(s) 569
KpnI GGTACC 3 cut(s) 138, 307, 327
Kzo9I GATC 2 cut(s) 643, 1092
LmnI GCTCC 3 cut(s) 4, 899, 1005
Lsp1109I GCAGC 3 cut(s) 79, 418, 622
MaeI CTAG 1 cut(s) 287
MaeIII GTNAC 2 cut(s) 79, 1085
MalI GATC 2 cut(s) 645, 1094
MbiI CCGCTC 1 cut(s) 894
MboI GATC 2 cut(s) 643, 1092
MboII GAAGA 9 cut(s) 167, 223, 259, 554, 727, 790, 793, 838, 920
MluCI AATT 9 cut(s) 101, 194, 476, 509, 605, 679, 722, 899, 1056
MlyI GAGTC 1 cut(s) 1006
MnlI CCTC 8 cut(s) 22, 117, 157, 240, 253, 791, 1111, 1159
Mph1103I ATGCAT 1 cut(s) 670
MroI TCCGGA 1 cut(s) 569
MroXI GAANNNNTTC 1 cut(s) 722
MseI TTAA 4 cut(s) 221, 329, 579, 672
MslI CAYNNNNRTG 1 cut(s) 645
MspI CCGG 3 cut(s) 528, 570, 865
MwoI GCNNNNNNNGC 1 cut(s) 92
NdeII GATC 2 cut(s) 643, 1092
NlaIII CATG 6 cut(s) 494, 538, 650, 688, 758, 973
NlaIV GGNNCC 5 cut(s) 46, 136, 305, 325, 954
NmuCI GTSAC 1 cut(s) 1085
NsiI ATGCAT 1 cut(s) 670
NspI RCATGY 1 cut(s) 538
PagI TCATGA 2 cut(s) 646, 754
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 2 cut(s) 106, 368
PinAI ACCGGT 1 cut(s) 864
PkrI GCNGC 3 cut(s) 94, 408, 637
PleI GAGTC 1 cut(s) 1005
PpsI GAGTC 1 cut(s) 1005
PsiI TTATAA 1 cut(s) 11
PspN4I GGNNCC 5 cut(s) 46, 136, 305, 325, 954
PspPI GGNCC 2 cut(s) 44, 952
RseI CAYNNNNRTG 1 cut(s) 645
SalI GTCGAC 1 cut(s) 181
SaqAI TTAA 4 cut(s) 221, 329, 579, 672
SatI GCNGC 3 cut(s) 93, 407, 636
Sau3AI GATC 2 cut(s) 643, 1092
Sau96I GGNCC 2 cut(s) 44, 952
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 1 cut(s) 1006
SinI GGWCC 2 cut(s) 44, 952
SmiMI CAYNNNNRTG 1 cut(s) 645
SmlI CTYRAG 1 cut(s) 994
SmoI CTYRAG 1 cut(s) 994
Sse9I AATT 9 cut(s) 101, 194, 476, 509, 605, 679, 722, 899, 1056
SsiI CCGC 1 cut(s) 892
SspMI CTAG 1 cut(s) 287
StyI CCWWGG 2 cut(s) 286, 395
TaaI ACNGT 2 cut(s) 425, 952
TaqI TCGA 4 cut(s) 27, 109, 182, 245
TasI AATT 9 cut(s) 101, 194, 476, 509, 605, 679, 722, 899, 1056
TatI WGTACW 2 cut(s) 743, 834
TfiI GAWTC 2 cut(s) 106, 368
Tru1I TTAA 4 cut(s) 221, 329, 579, 672
Tru9I TTAA 4 cut(s) 221, 329, 579, 672
TscAI CASTG 1 cut(s) 183
TseFI GTSAC 1 cut(s) 1085
TseI GCWGC 3 cut(s) 92, 406, 635
Tsp45I GTSAC 1 cut(s) 1085
TspDTI ATGAA 6 cut(s) 366, 370, 701, 719, 930, 954
TspGWI ACGGA 2 cut(s) 278, 1086
TspRI CASTG 1 cut(s) 183
VpaK11BI GGWCC 2 cut(s) 44, 952
XapI RAATTY 4 cut(s) 194, 476, 722, 899
XceI RCATGY 1 cut(s) 538
XmaJI CCTAGG 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 722
XspI CTAG 1 cut(s) 287
ZrmI AGTACT 1 cut(s) 745
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.