AT2G31420

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000766
Physical Location & Seq
Reverse (-)
13393364 .. 13394136
773 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31420.1

Sequence Viewer

Length: 630 bp
ATGGAGGCTTATGATATTTGTAAGTTTTGGAAGCTGGATATGTTGGAAGAATTTTCTCAAATAGTTCTCAAAGGAAAAAAGAAAATCATCCTCGACATCCCGAGCCCCTCTCAGCAGAACAAAAGCGACATCGTTTCGTTATGTCCTGCTGATAGTCCCGAAACATGCAATTCTTCGATACCAGACTGGCTCGTGAAAGTGATGAGAAAAGAGAATGGATACGATCCAAAGCTGATCATAAAGAGGAGGGTGCTGTGTACGACTGATCTCCGTAAAAACCAGGGGTGTCTCTCAATGCATCTCAGCAAATTAGAGAAGTCAGATTTTTTGACAGAGGACGAAACCAGATTCTTAGATGAAGACTTTTTAAAGGCTAAAAGGGATGGTTTGAAAGTGTTTTTGGTGGATCCTGAATCGGATAAGCATGTCGTTTATTTAAAGAAGTGGAATGGTAATACAGTTTGGAAATATGTGTTAAGTCATGGTTGGAACAATGTGATTGATAAAAAAATCTTCAAAGTCAATGACGTGATTGAAATCTGGTCCTTTCGAGATGGAAGTGGAAAACTTTGCTTTGCTCTCTCTTCTCCAACCCGTTGTGGTAGAAGCAGCTCTGGTCATTCCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

209

Amino Acids

24.04

Weight (kDa)

8.74

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 59 - 169 1.9e-42 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 218, 401, 414
AcsI RAATTY 1 cut(s) 50
AfaI GTAC 1 cut(s) 259
AgsI TTSAA 3 cut(s) 391, 517, 536
AjiI CACGTC 1 cut(s) 529
AjnI CCWGG 1 cut(s) 279
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 3 cut(s) 34, 232, 612
AluI AGCT 3 cut(s) 34, 232, 612
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 3 cut(s) 218, 401, 414
Ama87I CYCGRG 1 cut(s) 100
ApeKI GCWGC 1 cut(s) 609
ApoI RAATTY 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 543
AvaI CYCGRG 1 cut(s) 100
AvaII GGWCC 1 cut(s) 543
BamHI GGATCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 107
BauI CACGAG 1 cut(s) 191
BbsI GAAGAC 1 cut(s) 366
BbvI GCAGC 1 cut(s) 621
BccI CCATC 2 cut(s) 377, 548
BciT130I CCWGG 1 cut(s) 281
BciVI GTATCC 1 cut(s) 212
BclI TGATCA 1 cut(s) 234
BcoDI GTCTC 1 cut(s) 293
BfuI GTATCC 1 cut(s) 212
BisI GCNGC 1 cut(s) 610
BlsI GCNGC 1 cut(s) 611
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 1 cut(s) 543
BmeT110I CYCGRG 1 cut(s) 100
BmgBI CACGTC 1 cut(s) 529
BmgT120I GGNCC 1 cut(s) 543
BmiI GGNNCC 1 cut(s) 408
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 1 cut(s) 307
BpiI GAAGAC 1 cut(s) 366
BplI GAGNNNNNCTC 2 cut(s) 94, 126
BsaBI GATNNNNATC 1 cut(s) 536
BsaJI CCNNGG 1 cut(s) 280
Bse1I ACTGG 1 cut(s) 191
Bse8I GATNNNNATC 1 cut(s) 536
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 3 cut(s) 87, 96, 388
BseJI GATNNNNATC 1 cut(s) 536
BseMII CTCAG 2 cut(s) 125, 316
BseNI ACTGG 1 cut(s) 191
BseRI GAGGAG 1 cut(s) 259
BseXI GCAGC 1 cut(s) 621
BsiHKCI CYCGRG 1 cut(s) 100
BslFI GGGAC 1 cut(s) 141
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 141
BsoBI CYCGRG 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 107
Bsp143I GATC 4 cut(s) 223, 234, 265, 406
BspCNI CTCAG 2 cut(s) 124, 315
BspLI GGNNCC 1 cut(s) 408
BspPI GGATC 3 cut(s) 218, 401, 414
BsrI ACTGG 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 4 cut(s) 223, 234, 265, 406
BssSI CACGAG 1 cut(s) 191
Bst2BI CACGAG 1 cut(s) 191
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 1 cut(s) 589
BstDEI CTNAG 3 cut(s) 111, 302, 352
BstF5I GGATG 3 cut(s) 87, 96, 388
BstKTI GATC 4 cut(s) 226, 237, 268, 409
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 4 cut(s) 223, 234, 265, 406
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 2 cut(s) 168, 428
BstSCI CCNGG 1 cut(s) 279
BstV1I GCAGC 1 cut(s) 621
BstV2I GAAGAC 1 cut(s) 366
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BsuI GTATCC 1 cut(s) 212
BtrI CACGTC 1 cut(s) 529
BtsCI GGATG 3 cut(s) 87, 96, 388
Cfr13I GGNCC 1 cut(s) 543
Csp6I GTAC 1 cut(s) 258
CviAII CATG 3 cut(s) 165, 425, 482
CviJI RGCY 7 cut(s) 8, 34, 105, 190, 232, 374, 612
CviKI_1 RGCY 7 cut(s) 8, 34, 105, 190, 232, 374, 612
CviQI GTAC 1 cut(s) 258
DdeI CTNAG 3 cut(s) 111, 302, 352
DpnI GATC 4 cut(s) 225, 236, 267, 408
DpnII GATC 4 cut(s) 223, 234, 265, 406
DraI TTTAAA 2 cut(s) 369, 438
Eam1104I CTCTTC 1 cut(s) 589
EarI CTCTTC 1 cut(s) 589
Eco24I GRGCYC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 543
Eco88I CYCGRG 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 279
EcoT22I ATGCAT 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 107
FaeI CATG 3 cut(s) 168, 428, 485
FaiI YATR 9 cut(s) 12, 41, 142, 166, 239, 426, 471, 483, 628
FaqI GGGAC 1 cut(s) 141
FatI CATG 3 cut(s) 164, 424, 481
FbaI TGATCA 1 cut(s) 234
Fnu4HI GCNGC 1 cut(s) 610
FokI GGATG 3 cut(s) 74, 83, 395
FriOI GRGCYC 1 cut(s) 107
Fsp4HI GCNGC 1 cut(s) 610
GluI GCNGC 1 cut(s) 610
Hin1II CATG 3 cut(s) 168, 428, 485
HinfI GANTC 2 cut(s) 348, 413
Hpy166II GTNNAC 1 cut(s) 258
Hpy188I TCNGA 2 cut(s) 322, 418
Hpy188III TCNNGA 5 cut(s) 100, 158, 193, 410, 551
Hpy8I GTNNAC 1 cut(s) 258
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 2 cut(s) 168, 298
HpyF3I CTNAG 3 cut(s) 111, 302, 352
HpySE526I ACGT 1 cut(s) 528
Hsp92II CATG 3 cut(s) 168, 428, 485
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 4 cut(s) 223, 234, 265, 406
Lsp1109I GCAGC 1 cut(s) 621
LweI GCATC 1 cut(s) 307
MaeII ACGT 1 cut(s) 528
MalI GATC 4 cut(s) 225, 236, 267, 408
MboI GATC 4 cut(s) 223, 234, 265, 406
MboII GAAGA 5 cut(s) 59, 165, 371, 505, 576
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 3 cut(s) 50, 169, 308
MmeI TCCRAC 3 cut(s) 24, 467, 614
MnlI CCTC 5 cut(s) 101, 118, 237, 240, 328
Mph1103I ATGCAT 1 cut(s) 300
MseI TTAA 3 cut(s) 368, 437, 476
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
NdeII GATC 4 cut(s) 223, 234, 265, 406
NlaIII CATG 3 cut(s) 168, 428, 485
NlaIV GGNNCC 1 cut(s) 408
NsiI ATGCAT 1 cut(s) 300
NspI RCATGY 2 cut(s) 168, 428
PfeI GAWTC 2 cut(s) 348, 413
PkrI GCNGC 1 cut(s) 611
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 1 cut(s) 408
PspPI GGNCC 1 cut(s) 543
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 1 cut(s) 259
RsaNI GTAC 1 cut(s) 258
SaqAI TTAA 3 cut(s) 368, 437, 476
SatI GCNGC 1 cut(s) 610
Sau3AI GATC 4 cut(s) 223, 234, 265, 406
Sau96I GGNCC 1 cut(s) 543
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 107
SetI ASST 4 cut(s) 36, 234, 531, 614
SfaNI GCATC 1 cut(s) 307
SinI GGWCC 1 cut(s) 543
Sse9I AATT 3 cut(s) 50, 169, 308
StyD4I CCNGG 1 cut(s) 279
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 1 cut(s) 531
TaqI TCGA 3 cut(s) 93, 176, 550
TasI AATT 3 cut(s) 50, 169, 308
TfiI GAWTC 2 cut(s) 348, 413
Tru1I TTAA 3 cut(s) 368, 437, 476
Tru9I TTAA 3 cut(s) 368, 437, 476
TseI GCWGC 1 cut(s) 609
TspDTI ATGAA 1 cut(s) 372
TspGWI ACGGA 1 cut(s) 260
VpaK11BI GGWCC 1 cut(s) 543
XapI RAATTY 1 cut(s) 50
XceI RCATGY 2 cut(s) 168, 428
Zsp2I ATGCAT 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.