Rroxscaffold_4G00279400

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000766
Physical Location & Seq
Reverse (-)
2204412 .. 2205461
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00279400.1

Sequence Viewer

Length: 942 bp
ATGGCGCCGCTAGTGAGTATGGAAGACTTCATAGAAAAACAACTTCGACCTGGTTCAACCTTGAATATTTTGGGTCAGGTTGCTTGTGTTGCACGGGAGAAACTCAAGCAAAGGAGGAAGGAGAAAAGTATTAAAGTCAAAACTCAGATGGGTAGTAGTCCCAAGGTCAAAGAGCCCAATTATGTAGCTGAAACATATTCCAAAGAGACGGATGCTGATGTGAACAAAAGAAAGAGTCGAGAGGACGATACTGATCATCATGACAAGGGTTTTCAATTGAAACAACGGAAGACCATTTTTTTGCACGGGGAATTTGGTACTACCTCCAAGACTAAAGAATCAAATTCATCAATGGGAAAAAATGGGTTGACAAAGAAGAAGAGTATTGATGGTGGTGAGGATGGTAGAGCTTGGAGTTTGAAGCCGAAGCCGAAGAATATGAAGATTAAGAAGGAAAAGGGGTTGGGATTCAAGAGGCCTAAGGAGCATATGAGAGCCCCAAGATATAGTACTGATCCTAATGACTTGCCTGAAGACTTGAAAAGGAAGATTGAAACTTTGGAAGGTATTGAAAGTTTCGAAAAGGCTAAGATGACTATGGTGATAGAGAAGCATCTGTTCGAGACTGATCTGACTGAAAGCGCAAGCCGATTGTCAATGCCTCTGAAACAAATCAAACCGGAGAGTTTCTTGGACGAGGATGAGATAAAGTTTCTTGCATCTCAACAAGACTGGCCGGTTTCGGTTATAAGTCCCAAACTTAAGGACGAAAATCTAACTTTCAGGCAATGGAACATGAATAAGAATAACGGCAACGTTAGCTCAAGTTATGTGTTGAAAACAGGTTGGAACGAACTTCACAAGAAAAATGGTCTCCAAGTAAATGATGTAATCATTTCGAGATGTGGGAAACAACCTTCACTTTGCTCTTGTTCTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

313

Amino Acids

35.63

Weight (kDa)

9.51

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 197 - 286 2.1e-15 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 749
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 1 cut(s) 8
AclI AACGTT 1 cut(s) 816
AclWI GGATC 1 cut(s) 509
AcoI YGGCCR 1 cut(s) 734
AcsI RAATTY 2 cut(s) 311, 343
AcuI CTGAAG 1 cut(s) 552
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 319, 511
AflII CTTAAG 1 cut(s) 761
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 3 cut(s) 188, 410, 822
AluI AGCT 3 cut(s) 188, 410, 822
Alw26I GTCTC 3 cut(s) 200, 617, 878
AlwI GGATC 1 cut(s) 509
AoxI GGCC 2 cut(s) 476, 734
ApoI RAATTY 2 cut(s) 311, 343
ArsI GACNNNNNNTTYG 2 cut(s) 283, 315
AspLEI GCGC 2 cut(s) 7, 644
AsuHPI GGTGA 2 cut(s) 407, 613
AsuII TTCGAA 1 cut(s) 579
AxyI CCTNAGG 1 cut(s) 480
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 177, 499
BbsI GAAGAC 3 cut(s) 30, 296, 540
BccI CCATC 3 cut(s) 142, 383, 395
BceAI ACGGC 1 cut(s) 826
BciT130I CCWGG 1 cut(s) 51
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 3 cut(s) 200, 617, 878
BfaI CTAG 1 cut(s) 11
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 761
BisI GCNGC 1 cut(s) 8
BlsI GCNGC 1 cut(s) 9
BmcAI AGTACT 1 cut(s) 511
Bme1390I CCNGG 1 cut(s) 51
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 3 cut(s) 202, 622, 728
BpiI GAAGAC 3 cut(s) 30, 296, 540
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 2 cut(s) 89, 808
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 878
BsaJI CCNNGG 1 cut(s) 162
BsaWI WCCGGW 1 cut(s) 679
Bse118I RCCGGY 1 cut(s) 736
Bse1I ACTGG 1 cut(s) 737
Bse21I CCTNAGG 1 cut(s) 480
Bse3DI GCAATG 1 cut(s) 794
Bse8I GATNNNNATC 1 cut(s) 252
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 3 cut(s) 217, 406, 706
BseJI GATNNNNATC 1 cut(s) 252
BseMI GCAATG 1 cut(s) 794
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 737
BshFI GGCC 2 cut(s) 478, 736
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 2 cut(s) 680, 737
BslFI GGGAC 2 cut(s) 144, 738
BsmAI GTCTC 3 cut(s) 200, 617, 878
BsmBI CGTCTC 1 cut(s) 200
BsmFI GGGAC 2 cut(s) 144, 738
BsnI GGCC 2 cut(s) 478, 736
Bso31I GGTCTC 1 cut(s) 878
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 2 cut(s) 177, 499
Bsp143I GATC 3 cut(s) 253, 514, 628
BspACI CCGC 1 cut(s) 8
BspANI GGCC 2 cut(s) 478, 736
BspCNI CTCAG 1 cut(s) 157
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 509
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 761
BspTNI GGTCTC 1 cut(s) 878
BsrDI GCAATG 1 cut(s) 794
BsrFI RCCGGY 1 cut(s) 736
BsrI ACTGG 1 cut(s) 737
BssAI RCCGGY 1 cut(s) 736
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 3 cut(s) 253, 514, 628
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 51
Bst6I CTCTTC 1 cut(s) 374
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 761
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 1 cut(s) 646
BstDEI CTNAG 3 cut(s) 144, 480, 588
BstF5I GGATG 3 cut(s) 217, 406, 706
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 644
BstKTI GATC 3 cut(s) 256, 517, 631
BstMAI GTCTC 3 cut(s) 200, 617, 878
BstMBI GATC 3 cut(s) 253, 514, 628
BstMWI GCNNNNNNNGC 3 cut(s) 89, 484, 819
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstV2I GAAGAC 3 cut(s) 30, 296, 540
Bsu36I CCTNAGG 1 cut(s) 480
BsuRI GGCC 2 cut(s) 478, 736
BtsCI GGATG 3 cut(s) 217, 406, 706
Cac8I GCNNGC 1 cut(s) 646
CciI TCATGA 1 cut(s) 259
CfoI GCGC 2 cut(s) 7, 644
Cfr10I RCCGGY 1 cut(s) 736
CsiI ACCWGGT 1 cut(s) 49
Csp6I GTAC 2 cut(s) 318, 510
CviAII CATG 2 cut(s) 260, 796
CviQI GTAC 2 cut(s) 318, 510
DdeI CTNAG 3 cut(s) 144, 480, 588
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 255, 516, 630
DpnII GATC 3 cut(s) 253, 514, 628
EaeI YGGCCR 1 cut(s) 734
Eam1104I CTCTTC 1 cut(s) 374
EarI CTCTTC 1 cut(s) 374
Eco130I CCWWGG 1 cut(s) 162
Eco147I AGGCCT 1 cut(s) 478
Eco24I GRGCYC 2 cut(s) 177, 499
Eco31I GGTCTC 1 cut(s) 878
Eco57I CTGAAG 1 cut(s) 552
Eco81I CCTNAGG 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 2 cut(s) 177, 499
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 200
FaeI CATG 2 cut(s) 263, 799
FaqI GGGAC 2 cut(s) 144, 738
FatI CATG 2 cut(s) 259, 795
FauNDI CATATG 1 cut(s) 489
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 1 cut(s) 8
FokI GGATG 3 cut(s) 224, 413, 713
FriOI GRGCYC 2 cut(s) 177, 499
Fsp4HI GCNGC 1 cut(s) 8
FspBI CTAG 1 cut(s) 11
GlaI GCGC 2 cut(s) 6, 643
GluI GCNGC 1 cut(s) 8
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 478, 736
HapII CCGG 2 cut(s) 680, 737
HhaI GCGC 2 cut(s) 7, 644
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 263, 799
Hin6I GCGC 2 cut(s) 5, 642
HinP1I GCGC 2 cut(s) 5, 642
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HinfI GANTC 3 cut(s) 235, 338, 468
HpaII CCGG 2 cut(s) 680, 737
HphI GGTGA 2 cut(s) 407, 613
Hpy166II GTNNAC 2 cut(s) 223, 369
Hpy188I TCNGA 3 cut(s) 147, 633, 666
Hpy188III TCNNGA 5 cut(s) 239, 260, 472, 622, 900
Hpy8I GTNNAC 2 cut(s) 223, 369
HpyAV CCTTC 4 cut(s) 112, 445, 557, 927
HpyCH4IV ACGT 1 cut(s) 816
HpyCH4V TGCA 3 cut(s) 92, 304, 719
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 484, 819
HpyF3I CTNAG 3 cut(s) 144, 480, 588
HpySE526I ACGT 1 cut(s) 816
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 263, 799
HspAI GCGC 2 cut(s) 5, 642
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 3 cut(s) 253, 514, 628
LmnI GCTCC 1 cut(s) 484
LweI GCATC 3 cut(s) 202, 622, 728
MabI ACCWGGT 1 cut(s) 49
MaeI CTAG 1 cut(s) 11
MaeII ACGT 1 cut(s) 816
MalI GATC 3 cut(s) 255, 516, 630
MboI GATC 3 cut(s) 253, 514, 628
MboII GAAGA 8 cut(s) 35, 301, 388, 391, 445, 454, 545, 559
MfeI CAATTG 1 cut(s) 275
MhlI GDGCHC 2 cut(s) 177, 499
MluCI AATT 4 cut(s) 178, 275, 311, 343
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 244
MmeI TCCRAC 1 cut(s) 827
MnlI CCTC 7 cut(s) 108, 235, 334, 391, 468, 672, 691
MseI TTAA 3 cut(s) 132, 447, 762
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 2 cut(s) 680, 737
MspR9I CCNGG 1 cut(s) 51
MunI CAATTG 1 cut(s) 275
MvaI CCWGG 1 cut(s) 51
MwoI GCNNNNNNNGC 3 cut(s) 89, 484, 819
NarI GGCGCC 1 cut(s) 5
NdeI CATATG 1 cut(s) 489
NdeII GATC 3 cut(s) 253, 514, 628
NlaIII CATG 2 cut(s) 263, 799
NlaIV GGNNCC 1 cut(s) 6
NspV TTCGAA 1 cut(s) 579
PagI TCATGA 1 cut(s) 259
PceI AGGCCT 1 cut(s) 478
PfeI GAWTC 2 cut(s) 338, 468
PkrI GCNGC 1 cut(s) 9
PleI GAGTC 1 cut(s) 243
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 243
PsiI TTATAA 1 cut(s) 749
Psp1406I AACGTT 1 cut(s) 816
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 1 cut(s) 6
RsaI GTAC 2 cut(s) 319, 511
RsaNI GTAC 2 cut(s) 318, 510
SaqAI TTAA 3 cut(s) 132, 447, 762
SatI GCNGC 1 cut(s) 8
Sau3AI GATC 3 cut(s) 253, 514, 628
ScaI AGTACT 1 cut(s) 511
SchI GAGTC 1 cut(s) 244
ScrFI CCNGG 1 cut(s) 51
SduI GDGCHC 2 cut(s) 177, 499
SexAI ACCWGGT 1 cut(s) 49
SfaNI GCATC 3 cut(s) 202, 622, 728
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 579
SmlI CTYRAG 3 cut(s) 104, 761, 823
SmoI CTYRAG 3 cut(s) 104, 761, 823
Sse9I AATT 4 cut(s) 178, 275, 311, 343
SseBI AGGCCT 1 cut(s) 478
SsiI CCGC 1 cut(s) 8
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 67
SspMI CTAG 1 cut(s) 11
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 1 cut(s) 162
TaiI ACGT 1 cut(s) 819
TaqI TCGA 5 cut(s) 46, 238, 579, 621, 899
TasI AATT 4 cut(s) 178, 275, 311, 343
TatI WGTACW 1 cut(s) 509
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 2 cut(s) 338, 468
Tru1I TTAA 3 cut(s) 132, 447, 762
Tru9I TTAA 3 cut(s) 132, 447, 762
TspDTI ATGAA 4 cut(s) 19, 336, 455, 812
TspGWI ACGGA 2 cut(s) 224, 301
Vha464I CTTAAG 1 cut(s) 761
XapI RAATTY 2 cut(s) 311, 343
XspI CTAG 1 cut(s) 11
ZrmI AGTACT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.