MD01G1220100.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000766
Physical Location & Seq
Forward (+)
31230542 .. 31231306
765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1220100.v1.1.491

Sequence Viewer

Length: 765 bp
ATGATAGCCTTGCTTGAGGTTGCTAGGCTCGCTACCGAGGACCTGGTGCAGGAGGAGCTTATGAAGCAGAAGAGGAAGATCAAGAAGCCTATGCTGATCGATTGGGCGGCCAAGAAAAATAGGTCGTCGAATATTTCTTTCAAAAGAAACTGGGAGATGGTAGATGATCCTGGTGATGTTGGTTGGACGCCTGTGGAACTCAAGATGCAGAAGATGTCGTTGAAGAACAATAACAAGAAAGGCGCAGACACGATGAATATCGCTTTGAAGAAGAAAGAAACGATTAGTCCTTCTGCGCCACTTGGGTATCATGACTTGCCTGAAAAATTCAAGAGAGAAATTGAGAGGTTGGGCGGGACTAAAGTGGAGTTGGTGATAGAGAAACAATTGACCAAGACTGATCTACGTTCGGGTAGGATGTCAATGCCTCTAAACCAAATCGTTTCAATTAGTTTCTTGGAAGACGAAGATATGGAGATGCTTGAGAACGGTGAGACAATGACGGTTCGGTTGATAGACCCTGGTCTTGTACAAGGAGATATCAACTTGCGGCGGTTGGAAAAGAGAGAGGGGAAAAACCCGATATATGTTTTGAGGACTCAATGGGGGGATGTTGCTCGGAAAAACAAACTCAAAGCTGCCGATCTCGTTCAAGTTTGGTCGTTTCGGGTTAATCGAGCGCTTCACTTAGCCCTAGTTTTGGTTAAGAGCAGCAGTGTTGACGGCGATCGTCAGGAGATCATGGAAGAATGGAAGAGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

255

Amino Acids

29.35

Weight (kDa)

9.48

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 120 - 206 1.9e-09 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 107, 354, 550, 553
AclWI GGATC 1 cut(s) 161
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 1 cut(s) 326
AcyI GRCGYC 1 cut(s) 188
AfaI GTAC 1 cut(s) 531
AfeI AGCGCT 1 cut(s) 681
AfiI CCNNNNNNNGG 2 cut(s) 49, 700
AgsI TTSAA 6 cut(s) 142, 223, 268, 331, 447, 653
AjnI CCWGG 3 cut(s) 42, 169, 520
AluBI AGCT 2 cut(s) 58, 638
AluI AGCT 2 cut(s) 58, 638
Alw26I GTCTC 1 cut(s) 488
AlwI GGATC 1 cut(s) 161
Aor51HI AGCGCT 1 cut(s) 681
AoxI GGCC 1 cut(s) 108
ApeKI GCWGC 2 cut(s) 638, 711
ApoI RAATTY 1 cut(s) 326
AspLEI GCGC 3 cut(s) 245, 298, 682
AspS9I GGNCC 1 cut(s) 40
AsuHPI GGTGA 3 cut(s) 185, 385, 503
AvaII GGWCC 1 cut(s) 40
BaeI ACNNNNGTAYC 2 cut(s) 290, 323
BbsI GAAGAC 1 cut(s) 468
BbvI GCAGC 2 cut(s) 625, 723
BccI CCATC 1 cut(s) 151
BceAI ACGGC 1 cut(s) 739
BciT130I CCWGG 3 cut(s) 44, 171, 522
BcoDI GTCTC 1 cut(s) 488
BfaI CTAG 2 cut(s) 24, 695
BfoI RGCGCY 1 cut(s) 683
BisI GCNGC 4 cut(s) 108, 551, 639, 712
BlsI GCNGC 4 cut(s) 109, 552, 640, 713
Bme1390I CCNGG 3 cut(s) 44, 171, 522
Bme18I GGWCC 1 cut(s) 40
BmgT120I GGNCC 1 cut(s) 40
BmrFI CCNGG 3 cut(s) 44, 171, 522
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 2 cut(s) 195, 468
BmuI ACTGGG 1 cut(s) 160
BoxI GACNNNNGTC 1 cut(s) 522
BpiI GAAGAC 1 cut(s) 468
BpuEI CTTGAG 3 cut(s) 35, 185, 503
Bsa29I ATCGAT 1 cut(s) 99
BsaBI GATNNNNATC 1 cut(s) 257
BsaHI GRCGYC 1 cut(s) 188
BsaJI CCNNGG 2 cut(s) 36, 520
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 700
Bse1I ACTGG 1 cut(s) 155
Bse8I GATNNNNATC 1 cut(s) 257
BseBI CCWGG 3 cut(s) 44, 171, 522
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 2 cut(s) 36, 520
BseGI GGATG 2 cut(s) 423, 616
BseJI GATNNNNATC 1 cut(s) 257
BseLI CCNNNNNNNGG 2 cut(s) 49, 700
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 2 cut(s) 625, 723
BsgI GTGCAG 1 cut(s) 68
Bsh1285I CGRYCG 1 cut(s) 730
BshFI GGCC 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 99
BsiEI CGRYCG 1 cut(s) 730
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 2 cut(s) 49, 700
BsmAI GTCTC 1 cut(s) 488
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 1 cut(s) 110
Bsp1407I TGTACA 1 cut(s) 529
Bsp143I GATC 7 cut(s) 78, 96, 166, 400, 643, 727, 738
BspACI CCGC 4 cut(s) 107, 354, 550, 553
BspANI GGCC 1 cut(s) 110
BspDI ATCGAT 1 cut(s) 99
BspHI TCATGA 1 cut(s) 310
BspPI GGATC 1 cut(s) 161
BsrGI TGTACA 1 cut(s) 529
BsrI ACTGG 1 cut(s) 155
BssECI CCNNGG 2 cut(s) 36, 520
BssMI GATC 7 cut(s) 78, 96, 166, 400, 643, 727, 738
BssNI GRCGYC 1 cut(s) 188
Bst2UI CCWGG 3 cut(s) 44, 171, 522
Bst4CI ACNGT 2 cut(s) 491, 505
Bst6I CTCTTC 2 cut(s) 65, 749
BstACI GRCGYC 1 cut(s) 188
BstAUI TGTACA 1 cut(s) 529
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 1 cut(s) 688
BstENI CCTNNNNNAGG 1 cut(s) 47
BstF5I GGATG 2 cut(s) 423, 616
BstH2I RGCGCY 1 cut(s) 683
BstHHI GCGC 3 cut(s) 245, 298, 682
BstKTI GATC 7 cut(s) 81, 99, 169, 403, 646, 730, 741
BstMAI GTCTC 1 cut(s) 488
BstMBI GATC 7 cut(s) 78, 96, 166, 400, 643, 727, 738
BstMCI CGRYCG 1 cut(s) 730
BstMWI GCNNNNNNNGC 3 cut(s) 29, 55, 64
BstNI CCWGG 3 cut(s) 44, 171, 522
BstPAI GACNNNNGTC 1 cut(s) 522
BstSCI CCNGG 3 cut(s) 42, 169, 520
BstV1I GCAGC 2 cut(s) 625, 723
BstV2I GAAGAC 1 cut(s) 468
Bsu15I ATCGAT 1 cut(s) 99
BsuRI GGCC 1 cut(s) 110
BsuTUI ATCGAT 1 cut(s) 99
BtsCI GGATG 2 cut(s) 423, 616
BtsI GCAGTG 1 cut(s) 721
BtsIMutI CAGTG 1 cut(s) 721
Cac8I GCNNGC 1 cut(s) 30
CciI TCATGA 1 cut(s) 310
CfoI GCGC 3 cut(s) 245, 298, 682
Cfr13I GGNCC 1 cut(s) 40
ClaI ATCGAT 1 cut(s) 99
CseI GACGC 1 cut(s) 196
CsiI ACCWGGT 1 cut(s) 42
Csp6I GTAC 1 cut(s) 530
CviAII CATG 2 cut(s) 311, 742
CviJI RGCY 7 cut(s) 8, 28, 58, 88, 110, 638, 692
CviKI_1 RGCY 7 cut(s) 8, 28, 58, 88, 110, 638, 692
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 1 cut(s) 688
DpnI GATC 7 cut(s) 80, 98, 168, 402, 645, 729, 740
DpnII GATC 7 cut(s) 78, 96, 166, 400, 643, 727, 738
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 2 cut(s) 65, 749
EarI CTCTTC 2 cut(s) 65, 749
Eco32I GATATC 1 cut(s) 541
Eco47I GGWCC 1 cut(s) 40
Eco47III AGCGCT 1 cut(s) 681
EcoNI CCTNNNNNAGG 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 40
EcoRII CCWGG 3 cut(s) 42, 169, 520
EcoRV GATATC 1 cut(s) 541
FaeI CATG 2 cut(s) 314, 745
FaiI YATR 7 cut(s) 62, 92, 312, 473, 586, 588, 743
FaqI GGGAC 1 cut(s) 370
FatI CATG 2 cut(s) 310, 741
FauI CCCGC 1 cut(s) 347
Fnu4HI GCNGC 4 cut(s) 108, 551, 639, 712
FokI GGATG 2 cut(s) 430, 623
Fsp4HI GCNGC 4 cut(s) 108, 551, 639, 712
FspBI CTAG 2 cut(s) 24, 695
GlaI GCGC 3 cut(s) 244, 297, 681
GluI GCNGC 4 cut(s) 108, 551, 639, 712
HaeII RGCGCY 1 cut(s) 683
HaeIII GGCC 1 cut(s) 110
HgaI GACGC 1 cut(s) 196
HhaI GCGC 3 cut(s) 245, 298, 682
Hin1I GRCGYC 1 cut(s) 188
Hin1II CATG 2 cut(s) 314, 745
Hin6I GCGC 3 cut(s) 243, 296, 680
HinP1I GCGC 3 cut(s) 243, 296, 680
HincII GTYRAC 1 cut(s) 721
HindII GTYRAC 1 cut(s) 721
HinfI GANTC 1 cut(s) 598
HphI GGTGA 3 cut(s) 185, 385, 503
Hpy166II GTNNAC 1 cut(s) 721
Hpy188I TCNGA 1 cut(s) 621
Hpy188III TCNNGA 5 cut(s) 82, 202, 311, 331, 734
Hpy8I GTNNAC 1 cut(s) 721
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 1 cut(s) 300
HpyCH4III ACNGT 2 cut(s) 491, 505
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 2 cut(s) 49, 208
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 55, 64
HpyF3I CTNAG 1 cut(s) 688
HpySE526I ACGT 1 cut(s) 406
Hsp92I GRCGYC 1 cut(s) 188
Hsp92II CATG 2 cut(s) 314, 745
HspAI GCGC 3 cut(s) 243, 296, 680
Kzo9I GATC 7 cut(s) 78, 96, 166, 400, 643, 727, 738
LmnI GCTCC 1 cut(s) 55
Lsp1109I GCAGC 2 cut(s) 625, 723
LweI GCATC 2 cut(s) 195, 468
MabI ACCWGGT 1 cut(s) 42
MaeI CTAG 2 cut(s) 24, 695
MaeII ACGT 1 cut(s) 406
MalI GATC 7 cut(s) 80, 98, 168, 402, 645, 729, 740
MboI GATC 7 cut(s) 78, 96, 166, 400, 643, 727, 738
MboII GAAGA 9 cut(s) 82, 88, 223, 235, 280, 283, 473, 479, 758
MfeI CAATTG 1 cut(s) 386
MluCI AATT 4 cut(s) 326, 339, 386, 447
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 2 cut(s) 164, 537
MnlI CCTC 9 cut(s) 10, 31, 46, 66, 339, 438, 562, 588, 750
MseI TTAA 2 cut(s) 672, 705
MspR9I CCNGG 3 cut(s) 44, 171, 522
MunI CAATTG 1 cut(s) 386
MvaI CCWGG 3 cut(s) 44, 171, 522
MwoI GCNNNNNNNGC 3 cut(s) 29, 55, 64
NdeII GATC 7 cut(s) 78, 96, 166, 400, 643, 727, 738
NlaIII CATG 2 cut(s) 314, 745
PagI TCATGA 1 cut(s) 310
PcsI WCGNNNNNNNCGW 1 cut(s) 673
PkrI GCNGC 4 cut(s) 109, 552, 640, 713
Ple19I CGATCG 1 cut(s) 730
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
PpuMI RGGWCCY 1 cut(s) 40
PshAI GACNNNNGTC 1 cut(s) 522
Psp5II RGGWCCY 1 cut(s) 40
Psp6I CCWGG 3 cut(s) 42, 169, 520
PspGI CCWGG 3 cut(s) 42, 169, 520
PspPI GGNCC 1 cut(s) 40
PspPPI RGGWCCY 1 cut(s) 40
PvuI CGATCG 1 cut(s) 730
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
SaqAI TTAA 2 cut(s) 672, 705
SatI GCNGC 4 cut(s) 108, 551, 639, 712
Sau3AI GATC 7 cut(s) 78, 96, 166, 400, 643, 727, 738
Sau96I GGNCC 1 cut(s) 40
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 3 cut(s) 44, 171, 522
SetI ASST 8 cut(s) 21, 45, 60, 125, 350, 409, 640, 761
SexAI ACCWGGT 1 cut(s) 42
SfaNI GCATC 2 cut(s) 195, 468
SinI GGWCC 1 cut(s) 40
SmlI CTYRAG 3 cut(s) 14, 200, 482
SmoI CTYRAG 3 cut(s) 14, 200, 482
Sse9I AATT 4 cut(s) 326, 339, 386, 447
SsiI CCGC 4 cut(s) 107, 354, 550, 553
SspI AATATT 1 cut(s) 133
SspMI CTAG 2 cut(s) 24, 695
StyD4I CCNGG 3 cut(s) 42, 169, 520
TaaI ACNGT 2 cut(s) 491, 505
TaiI ACGT 1 cut(s) 409
TaqI TCGA 3 cut(s) 99, 128, 676
TasI AATT 4 cut(s) 326, 339, 386, 447
TatI WGTACW 1 cut(s) 529
TauI GCSGC 2 cut(s) 110, 553
Tru1I TTAA 2 cut(s) 672, 705
Tru9I TTAA 2 cut(s) 672, 705
TscAI CASTG 1 cut(s) 721
TseI GCWGC 2 cut(s) 638, 711
TspDTI ATGAA 2 cut(s) 77, 269
TspRI CASTG 1 cut(s) 721
VpaK11BI GGWCC 1 cut(s) 40
XagI CCTNNNNNAGG 1 cut(s) 47
XapI RAATTY 1 cut(s) 326
XspI CTAG 2 cut(s) 24, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.